AlfalfaGEDB | MS.gene000032

M. sativa cultivar XinJiangDaYe / MS.gene000032

KEGG: K01188
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00232.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000032.t1	XP_003599468.1	95.9	492	19	1	1	491	1	492	3.40E-284	986.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000032.t1	Q9FIW4	69.7	472	141	2	22	491	18	489	5.9e-208	724.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000032.t1	A8TVR1	95.9	492	19	1	1	491	1	492	2.5e-284	986.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
MS.gene000032	MS.gene056362	PPI
MS.gene000032	MS.gene57366	PPI
MS.gene000032	MS.gene002906	PPI
MS.gene000032	MS.gene81847	PPI
MS.gene000032	MS.gene96802	PPI
MS.gene000032	MS.gene07214	PPI
MS.gene000032	MS.gene05298	PPI
MS.gene000032	MS.gene064123	PPI
MS.gene000032	MS.gene031517	PPI
MS.gene000032	MS.gene003897	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000032.t1	MTR_3g033810	95.935	492	19	1	1	491	1	492	0.0	975
MS.gene000032.t1	MTR_3g033790	86.965	491	62	2	1	491	1	489	0.0	889
MS.gene000032.t1	MTR_4g083000	52.092	478	215	4	18	482	26	502	0.0	517
MS.gene000032.t1	MTR_4g081530	49.582	478	226	5	19	482	23	499	1.65e-169	488
MS.gene000032.t1	MTR_6g078450	50.522	479	218	6	20	482	36	511	7.16e-168	484
MS.gene000032.t1	MTR_5g044000	48.096	499	223	7	18	481	23	520	4.49e-166	479
MS.gene000032.t1	MTR_0110s0040	50.311	483	204	7	20	481	43	510	1.01e-163	473
MS.gene000032.t1	MTR_0110s0030	49.896	483	206	7	20	481	43	510	3.53e-163	472
MS.gene000032.t1	MTR_2g098520	48.225	479	233	6	18	482	32	509	2.18e-161	467
MS.gene000032.t1	MTR_3g026280	48.958	480	221	8	18	481	20	491	2.50e-159	461

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000032.t1	AT5G36890	69.703	472	141	2	22	491	18	489	0.0	708
MS.gene000032.t1	AT5G36890	70.000	470	138	3	22	488	18	487	0.0	704
MS.gene000032.t1	AT1G26560	51.046	478	219	6	18	481	30	506	6.59e-175	501
MS.gene000032.t1	AT3G18080	50.210	476	218	7	20	482	39	508	5.07e-165	476
MS.gene000032.t1	AT5G54570	48.637	477	229	6	20	481	30	505	1.49e-162	471
MS.gene000032.t1	AT3G18070	48.734	474	220	7	20	482	30	491	4.12e-162	468
MS.gene000032.t1	AT3G18070	48.945	474	225	7	20	482	30	497	4.80e-161	466
MS.gene000032.t1	AT2G44480	44.960	496	257	7	5	486	20	513	5.27e-155	451
MS.gene000032.t1	AT2G44480	44.960	496	257	7	5	486	51	544	1.13e-154	451
MS.gene000032.t1	AT3G60140	46.392	485	233	8	19	482	25	503	2.46e-153	449

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Find 110 sgRNAs with CRISPR-Local

Find 361 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACTCGACAAAAGCAA+TGG	0.611643	3.1:+24548802	MS.gene000032:CDS
AAAGATGGGGCAAACAAGAA+GGG	0.597402	3.1:+24554036	MS.gene000032:CDS
AAATAGAAACAGCTTCTGCA+TGG	0.554274	3.1:-24551151	None:intergenic
AACCAATATGCAGAAGACTT+TGG	0.453110	3.1:-24554000	None:intergenic
AAGTACCTGATAAGCAGAAG+TGG	0.596695	3.1:-24547880	None:intergenic
AATAGAAACAGCTTCTGCAT+GGG	0.599937	3.1:-24551150	None:intergenic
ACCAATATGCAGAAGACTTT+GGG	0.355117	3.1:-24553999	None:intergenic
ACCAGCCAAGACGAAAATCT+AGG	0.501744	3.1:-24551493	None:intergenic
ACCCAAAGTCTTCTGCATAT+TGG	0.333496	3.1:+24553998	MS.gene000032:CDS
ACCCACCAACTGCGGTTTGA+AGG	0.428874	3.1:-24551046	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATACTCGACAAAAGCAA+TGG	+	chr3.1:24548802-24548821	MS.gene000032:CDS	30.0%
AACGTTAAACATAAAGACTC+CGG	-	chr3.1:24548068-24548087	None:intergenic	30.0%
AAGTATTAAAGGTTATGACG+TGG	+	chr3.1:24550731-24550750	MS.gene000032:intron	30.0%
AATCCTGATTAGATAGCATA+AGG	+	chr3.1:24552892-24552911	MS.gene000032:intron	30.0%
ACATTACATATCCTTTCCTA+TGG	-	chr3.1:24549774-24549793	None:intergenic	30.0%
AGAACGGAAATATGTGTAAA+TGG	+	chr3.1:24552775-24552794	MS.gene000032:intron	30.0%
ATCAATGCTCTTCTTGAAAA+AGG	+	chr3.1:24550450-24550469	MS.gene000032:CDS	30.0%
ATTACTTGACAACTTCGAAT+GGG	+	chr3.1:24553912-24553931	MS.gene000032:CDS	30.0%
ATTAGAAACATAGAAAACCG+TGG	-	chr3.1:24548462-24548481	None:intergenic	30.0%
ATTGATCTTATAGCCAAGTT+GGG	+	chr3.1:24549248-24549267	MS.gene000032:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	24547780	24554068	24547780	ID=MS.gene000032
chr3.1	mRNA	24547780	24554068	24547780	ID=MS.gene000032.t1;Parent=MS.gene000032
chr3.1	exon	24547780	24547896	24547780	ID=MS.gene000032.t1.exon1;Parent=MS.gene000032.t1
chr3.1	CDS	24547780	24547896	24547780	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24548652	24548718	24548652	ID=MS.gene000032.t1.exon2;Parent=MS.gene000032.t1
chr3.1	CDS	24548652	24548718	24548652	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24548800	24548858	24548800	ID=MS.gene000032.t1.exon3;Parent=MS.gene000032.t1
chr3.1	CDS	24548800	24548858	24548800	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24549242	24549317	24549242	ID=MS.gene000032.t1.exon4;Parent=MS.gene000032.t1
chr3.1	CDS	24549242	24549317	24549242	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24550397	24550471	24550397	ID=MS.gene000032.t1.exon5;Parent=MS.gene000032.t1
chr3.1	CDS	24550397	24550471	24550397	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24550550	24550637	24550550	ID=MS.gene000032.t1.exon6;Parent=MS.gene000032.t1
chr3.1	CDS	24550550	24550637	24550550	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24550965	24551190	24550965	ID=MS.gene000032.t1.exon7;Parent=MS.gene000032.t1
chr3.1	CDS	24550965	24551190	24550965	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24551398	24551513	24551398	ID=MS.gene000032.t1.exon8;Parent=MS.gene000032.t1
chr3.1	CDS	24551398	24551513	24551398	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24551811	24552025	24551811	ID=MS.gene000032.t1.exon9;Parent=MS.gene000032.t1
chr3.1	CDS	24551811	24552025	24551811	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24552108	24552142	24552108	ID=MS.gene000032.t1.exon10;Parent=MS.gene000032.t1
chr3.1	CDS	24552108	24552142	24552108	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24552250	24552349	24552250	ID=MS.gene000032.t1.exon11;Parent=MS.gene000032.t1
chr3.1	CDS	24552250	24552349	24552250	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24553041	24553149	24553041	ID=MS.gene000032.t1.exon12;Parent=MS.gene000032.t1
chr3.1	CDS	24553041	24553149	24553041	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1
chr3.1	exon	24553876	24554068	24553876	ID=MS.gene000032.t1.exon13;Parent=MS.gene000032.t1
chr3.1	CDS	24553876	24554068	24553876	ID=cds.MS.gene000032.t1;Parent=MS.gene000032.t1

Gene Sequence

>MS.gene000032

ATGGTAAAGAAAGAAGAATTCATGAGAGAAAACGGCTTTGAGAAAGGACAAGCAGAAGTCTCACGCAGTGACTTCCCATCTGACTTCGTCTTCGGAGTCGCCACTTCTGCTTATCAGGTACTTCATCAATGCAGATTCACATTCATTATTTGCTAAACTTTCAATTTGATCTCTAAACTATTGATGCTTTTTCGATGATTTGGTGCATGATGCTAACTTGATGAATCACTGAATTAGTCTTTGACTTTGTAAAAGACTTTCAATATTTTAAAAGGTCAAATACATCCGGAGTCTTTATGTTTAACGTTTGTAATAGACTGTCCTTTTAGTTTTTTTTTTTTTTTTTGCACGATTACCATCTTTTTATTTAATTAATTTTCAAAATAGCGAGCTTGAAATTTGAACTGTCGGGTATAAATGCAACGCGAGTCCATTTATCTTGATCTCGAAATTGAAGATTTACTGTTGATTATTTATTACTTTGTGGTGAAAAAAATGTTAATTTGAGCTTGCTTTAATTTCTTTGCTTTTGCTCTCTCTTTGTTATAGTTGTTTTTGTGGAACAAAATGACACTGCATTTTATATATACTTTTTGTCTTGTTTTGTTCTGCTCTTAACTTGCACCAAAGATGCATATAACAAGACTTAGACGATCTCTTCTGTGTTATTCTAACCTATCCACGGTTTTCTATGTTTCTAATTTTTATTTTTTTTGTTGATCCTATACTTTTGTAAAAGTTAATAACAAATTATTCTGTTGTTGATCCTATGTTTTTAACTGTTGCCCGAATGAGTAATGGTATTGGTAGGCGATTATATGGATCCGAGTTAATTTTTCTCTGAAACTATAACTTGTTGCTTATTTCTATAGATAGAAGGTGCTAGCAAGGAGGGTGGTAGAGGTCCTAGTATATGGGATGCCTATGCTTATACTGAAGGTATGTGAATGATTTCCTCAATTTGCTCAAATTGTGCTCCTTGATGCTTCATTACTTACTATTTTTGTGTTGTTTTGGTAGGAAAAATACTCGACAAAAGCAATGGCGATGTAGCAGTTGATCATTATCATCGTTATAAGGTCAGTGATGTCTTTCTTTTGCCTAAATATTGTGTATGCCAATTGTCTAGCATTGGTCTTTTGCTTAGTTATTATCATCGTATATGACGTCGTTTAATCTATGTAGCTGACTCAACCAAGTGAGGAAAGATTTTTGTGGTTGTACATATTGTCTTTCTGGATTGATTTTGGTTTAGAAATTAATATTTGCATTTAAATGTTGCTTTACAGGAGACTGGTTAATTTTGTCCCCATATTGAGTCTATTTTTAAGCACGAATGCGTGAAAGTGATATTCAATCTTTGTAATTTTTTCATATCTTACTATTATGTCTGTAACTGTCAAGATCATGTCGTGCACGTAAATTTCAGATTTTAACATTCTTAATGTAACTATGTGAACAGGAAGATATTGATCTTATAGCCAAGTTGGGTTTCAACGCGTACAGATTTTCAATTTCTTGGTCTCGTATTTTCCCTGGTATGTTTTATTAGTGATGAATTCTAAATACATATCTTCTTTATATGTTTCAGTGTTAATTAAATTATTTCCATGTCCATGAGAGCCTTAAGCCTAGAAGTTTATCAACTCCTCTGTCATCAATACTTGGTCAATAGTTAATATTTGCATCTTCGGCTCATAAAGACATGTTTACAACAGATGATTAAAGGCGTAAATTAAGTCTTTTCTGGGAGGGAATGACCCTGGCCCAACCGATAAGATGGTAAAGTAAAAAAGATAGGAGAGGTTTTTCAAATTTTAAAGATAGGAGAGGTCTTTCAATAAAATATAACACTTTTTCGTCTTACCGCCAGATAGGAGAGAGTGCAATTTTTTTATACAGTGGGGAGGAATATGTAATGTGCAAATTTCTCAAGAAAATAGTGTTACGTATAGCAAAATAAAAAAGAAGCAGGGAATACACAGGCTTTACCATAGGAAAGGATATGTAATGTGCAAATTTCTTGTGTCTCATTTGAAATCTGATAAAAAATGTGACACACACTACTGGTAAGAAATGATGACCCCTTAAACAAGTTGTTGGCTAATATAGGAGATGGTGCGAGAACTGTTGAGAACGCGTGATATGTACCGTAAACTCTGCACAAGAAATATGGAATACAACTTCATGAACTACGAAATTAAGCTCAGAAACTACAAGAAAAGAAAGAAGAGAAACTAACAGACTTAAACCATCCGGAACCTCCCAAGCCAAATGGCTCCAAACCTTTACCAAATTAATTATCCACTACTTACATCGTTCACATATTCCCATTCTAACTAACCTGCTACATGGCTAGTTTGTTACAATTTTCACCCCATACTCTCACACTACAGAGTTTCTTTTTTAATTTCTTATTAATCTATCAAGATTGCTAAATGTTTTGAGGATAATAACATCGTTCGCCTTGTCCCTAAAACCATTATTTTTAGGTTTTACATATACGATAACAGTTCTTACTTCTTACATTAATATGTTGATTTGGTTTGTCGTGCTATTGATAATTACAGAATCTTTAGATTTCATTTTATTATATTGCAATGTTTTATGGGTGTAGATGGCTTAGGAACCAATGTCAATGATGAAGGCATAACATTTTACAACAACATTATCAATGCTCTTCTTGAAAAAGGTATTGTCTACAAAAACCTGCTTTGCCTTAATATTTTTATCTCTTCTATCAACCATATATATTAATCATTTCCCACAGGTATTCAACCTTTTGCAACTTTGTACCATTGGGATCTTCCACTGCATCTTGATGAGTCAATGGGAGGATGGTTGAATAAAAATATCATGTAGGTATCATCTAATTAACTATTATTATTTTTTTATTTTACTATATATTTCTTTCTAGATAATGCAAAAATTGATTTGTATGATGTGCTTGAAAGTATTAAAGGTTATGACGTGGTTGAATAAAAAAATCATGTAGGTATCATCTAACTGAGTATGGTTAGAAGCTTCCTTACGAAGTATCTGATGCCAAGTATATATTTAATTTGTTATTGGACCTTACCTCTAAATTTTTTGATAAATATGTTTATTGATAAAAGTGTTCAAATTCATGTTTGATTTCCAATATTCTATTACTGAATGCAGTGTACATATGTGGCATGTTGCAGAGAATATTTTGCAGTCTATGCAGATACTTGCTTTGCAAGTTTTGGCGATAGAGTGAAAAACTGGATTACTATTAACGAGCCCCTTCAAACCGCAGTTGGTGGGTATGATGCTGGAGTTAATGCTCCTGGAAGAAGTGAAAATAGATCAGTAGAACCATACTTAGCAGCACATCATCAAATCTTGGCCCATGCAGAAGCTGTTTCTATTTACCGAAGCAAGTACAAGGTCAAACACTATACTTTTTCCTACTTGTTAGATTACATTTATTTTCTTTAGTTTGTTATATTTTTCTGCTATCTCCTACTCGAAGAATATATTTTCAATCATCCTACATGGCAACTACTCTCATTAATATCATAACTTCGCGTGCACAGTTTTATTATAAGACTTGCACAATAGTTGAGGTAAAACTTTATTCTCTTGATGTTCCAGGATAAGCAAGGGGGACAAGTGGGGCTGGTTGTAGACTCTGAATGGGCAGAGCCAAACTCTGATAAGATTGAAGATAAATCCGCTGCTGCGAGACACCTAGATTTTCGTCTTGGCTGGTAATTCCTATACATACATTTGTTTTATATTATTAGTTTTCATAAGATTTCCATAGATTTTCCACTTGATATAAATGCATGAAGATGTGTGGACACAAACTTACAAAGGTATACATACATTTTTGCGTTTAACGTTGGAAATGCTCTTCTAGTTTCTTCTATTCTCTAAATATTAAACTCAAAACAGCAAAAGGTCTTGGTTTTTCTTCGATGCTCGTGAACACTAGAAATTGAAGGTTGAGGATATTTAACCTTGGTGTAAGATGCATGCTTATGATAGAATATATTCTTAACCAGGTTCTTGCATCCACTATATTATGGAGACTATCCTGAAGTTATGCGTGAACGACTTGGAGACCGACTTCCCAAATTCTCGGAGGAGGATAAAAAATTTCTTTTGAATTCTTTGGACTTCATTGGCCTAAATCACTATACAACGAGGTTAATTTCACATGTGACAGAAAGCACCGAAGAATGTCATTACGACAAGGCACAACAGCTGGACAGAATTGGTAAATAATTCGATTTTCGTAGAATTTTCTAAACTATATATCCAAAATGAATCTTCCTTGATGATTGAGCTGTTTTTCACAGTTGAATGGGAAGGCGGGGAGCTCATTGGTGAGAAGGTATATACCATCTATGTCTCTCTGTCGGTGTATTGTAATATATACACACACACACACACACAGACAAGACCAAAGATGCAAGTTTATATTCTATGTGCATATAACAGGCAGCATCAGAGTGGCTATATGTTGTTCCTTGGGGTCTTCGGAAGATTATTAATTACATATCGCAAAAATATGCTGCACCGATATATGTCACCGAGAATGGTATGTGACTTTCTTTTCCTTCTTGTCCACTTTTAGAAAACAAATAGGGAATGAACCACTCGATAGAAAATTGAGGGAGAAATCATGTGTGTGTCTATATATCTCTTTTGAATCTTCTGTAGTGTTTGGCATGTACGTATAATGAAACAGAACCGCCTTTTATGTTTTGTTCTCATTGGTGCCAGTATTTATGTAAAGCCAAAATGTATCACTTTTTTCCTACATGTTCTGTTCTATTTTCTTTGATTCCAACCCATTTGTGTAGAACCAAAATGTATCACTTTTCTTACCTTTTGTCCCCTTCTATCGTTTATTAATATACACCAATTTATCCTGTTATGTATTGTTTCTAACTTGTCTCTCAAATGAATGGATATTGAAAGTATATACGTTATTAAGTCTGAAATAATCGTGAACTATAAAAAAGAACGGAAATATGTGTAAATGGACGAGTGTGAGTTATGTTATATGGCTCAACAAGGAAGAGTGGATGCAATGTGTATTTTGAGAGCTGCAACCTAGGAATATGACTGGCATGGCAAAATCCTGATTAGATAGCATAAGGTTGAAGATATGTCAACAATAGGATCCGCGGCTCTGATTTAATAGATGATAAACAGATTTTATATGACTGGACCAGAACTTTATAAAATGGTTCTCGTATTTATAAGGCTGTCTTTCCATTTAACAGGTATGGATGATGAAGACAATGATAGCTTGCCACTAAATGAGATGCTAGATGACAAACTGCGAGTTCGCTATTACAAGGGATACCTTGCATCACTTGTGCAGGCTATAAAGTAAGATTTCCATTTTCTTCTCACACACACCTCATCTACGAGTTAAACCAGTTTATTTTTCAAATTATCGAGTATTATGAATAGAGAGAATTGAATGGAAATAGATTTCTCACTAATGAATTAAGCTTAGTTGAAGTTGTATATATACACAAGAGTGTCTCTATTTTAGCTTATCATGTGTAACAAACAGGAAATCATTAACTCTTAACTATCTAATGATTACTTGTTGCACAAGTAATGATTCTCTAACTAATTGTCAAACTATCTAATGATTACTTGCTGCACAAGTAATCTTTGCTTAGCTGATATGGTGTGATAATAAGTAATATAAAAACGAAGTATATGGTTTAATGTTTTCTTTGAACCTTATTATGCATATGTCTGTCACTGAACAAATTTATAACAATACAACTTTGAGAACTTGAAATTGTTATAAATGATGATTGTCCTATTTGCTTAAAAACATTGAAAGTTTAGAACAGGGTCAGACACTTGGTCTTTGTCCATTGAAAATCATAAAAGCCTTTCTGATCTATACATGATTTTGAAAAGAGAATAAAAAAAAAATTAAGGGAACACTTAATATACAATTTTCACATCATTATCAAATACAGTTTTCACCAGCCTAACATAACAACTGACCGAGTAAAATCGTTTGAACTTCAAAACAGAATAAGAATCTTAATCAAACACGCCTAAAGTTTTGTAAATCATTTGAATGCACAGGGATGGAGCTGATGTGAGAGGACACTTTGCATGGTCATTACTTGACAACTTCGAATGGGCTCAAGGTTATACCAAACTCTTCGGATTGGTCTATGTAGATTACAAAAATGGACTCACTAGGCACCCAAAGTCTTCTGCATATTGGTTTTCGCGGTTTCTTAAAGATGGGGCAAACAAGAAGGGTAAAGAATAG

Protein sequence

>MS.gene000032.t1

MVKKEEFMRENGFEKGQAEVSRSDFPSDFVFGVATSAYQIEGASKEGGRGPSIWDAYAYTEGKILDKSNGDVAVDHYHRYKEDIDLIAKLGFNAYRFSISWSRIFPDGLGTNVNDEGITFYNNIINALLEKGIQPFATLYHWDLPLHLDESMGGWLNKNIIEYFAVYADTCFASFGDRVKNWITINEPLQTAVGGYDAGVNAPGRSENRSVEPYLAAHHQILAHAEAVSIYRSKYKDKQGGQVGLVVDSEWAEPNSDKIEDKSAAARHLDFRLGWFLHPLYYGDYPEVMRERLGDRLPKFSEEDKKFLLNSLDFIGLNHYTTRLISHVTESTEECHYDKAQQLDRIVEWEGGELIGEKAASEWLYVVPWGLRKIINYISQKYAAPIYVTENGMDDEDNDSLPLNEMLDDKLRVRYYKGYLASLVQAIKDGADVRGHFAWSLLDNFEWAQGYTKLFGLVYVDYKNGLTRHPKSSAYWFSRFLKDGANKKGKE