AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042247

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042247.t1 MTR_4g131800 93.866 538 32 1 1 538 493 1029 0.0 1068
MS.gene042247.t1 MTR_2g022760 72.421 504 137 2 1 502 12 515 0.0 771
MS.gene042247.t1 MTR_4g131810 70.798 476 136 2 28 500 32 507 0.0 718
MS.gene042247.t1 MTR_2g022710 63.655 498 179 1 5 500 14 511 0.0 672
MS.gene042247.t1 MTR_2g022760 74.515 412 105 0 91 502 1 412 0.0 657
MS.gene042247.t1 MTR_2g022710 63.348 442 160 1 5 444 14 455 0.0 588
MS.gene042247.t1 MTR_2g022710 63.348 442 160 1 5 444 14 455 0.0 587
MS.gene042247.t1 MTR_2g022710 64.390 410 146 0 91 500 1 410 0.0 567
MS.gene042247.t1 MTR_2g022710 65.517 406 138 1 5 408 14 419 0.0 559
MS.gene042247.t1 MTR_2g079580 49.621 528 257 3 1 527 8 527 0.0 558
MS.gene042247.t1 MTR_2g079550 48.851 522 258 3 1 521 6 519 0.0 546
MS.gene042247.t1 MTR_2g022710 64.721 394 137 1 5 396 14 407 0.0 535
MS.gene042247.t1 MTR_2g022710 65.405 370 126 1 5 372 14 383 1.01e-177 507
MS.gene042247.t1 MTR_4g083000 48.560 486 241 5 20 500 24 505 5.67e-164 475
MS.gene042247.t1 MTR_2g079580 52.567 409 192 2 1 408 8 415 7.20e-163 469
MS.gene042247.t1 MTR_2g098520 47.789 475 242 5 29 499 39 511 4.29e-160 466
MS.gene042247.t1 MTR_4g066630 49.260 473 234 5 29 497 44 514 9.56e-159 462
MS.gene042247.t1 MTR_2g098540 48.211 475 240 5 29 499 36 508 1.09e-158 462
MS.gene042247.t1 MTR_4g066210 48.954 478 238 5 29 502 48 523 1.70e-158 462
MS.gene042247.t1 MTR_0110s0040 47.890 474 238 5 29 500 48 514 4.85e-158 461
MS.gene042247.t1 MTR_6g078330 47.119 486 251 6 20 501 33 516 6.53e-158 461
MS.gene042247.t1 MTR_6g078450 48.421 475 237 7 29 498 41 512 1.73e-157 459
MS.gene042247.t1 MTR_0110s0030 47.789 475 237 5 29 500 48 514 1.17e-156 457
MS.gene042247.t1 MTR_4g066240 48.125 480 243 5 23 498 35 512 3.36e-156 456
MS.gene042247.t1 MTR_2g098790 45.833 504 263 6 3 498 13 514 7.11e-156 455
MS.gene042247.t1 MTR_4g066580 47.789 475 242 5 29 499 44 516 1.24e-155 455
MS.gene042247.t1 MTR_3g026280 46.450 493 253 6 8 496 6 491 1.42e-155 453
MS.gene042247.t1 MTR_6g078380 49.057 477 235 8 29 500 41 514 1.46e-155 454
MS.gene042247.t1 MTR_4g081530 47.581 496 250 6 5 496 9 498 7.92e-155 452
MS.gene042247.t1 MTR_4g066240 48.025 481 243 6 23 498 35 513 2.37e-154 451
MS.gene042247.t1 MTR_4g066590 47.780 473 241 5 29 497 38 508 1.54e-153 449
MS.gene042247.t1 MTR_5g044000 46.200 500 240 8 23 496 24 520 3.15e-153 449
MS.gene042247.t1 MTR_4g023000 44.112 501 270 5 1 498 1 494 8.84e-152 444
MS.gene042247.t1 MTR_3g028720 46.316 475 249 5 29 499 43 515 8.71e-151 442
MS.gene042247.t1 MTR_2g098540 47.505 461 236 5 43 499 6 464 1.64e-150 439
MS.gene042247.t1 MTR_4g066640 47.170 477 240 7 29 499 44 514 3.88e-148 435
MS.gene042247.t1 MTR_3g026400 46.458 480 251 4 23 500 36 511 4.92e-148 435
MS.gene042247.t1 MTR_5g071520 47.826 483 237 6 29 498 44 524 9.74e-148 434
MS.gene042247.t1 MTR_3g026620 46.791 483 251 4 20 500 34 512 6.79e-147 432
MS.gene042247.t1 MTR_3g033790 45.228 482 248 5 17 496 12 479 9.44e-145 426
MS.gene042247.t1 MTR_4g066340 48.380 432 218 4 70 498 10 439 9.88e-145 424
MS.gene042247.t1 MTR_4g066330 46.512 473 225 5 29 498 46 493 2.68e-144 425
MS.gene042247.t1 MTR_3g033810 46.170 483 233 6 22 496 19 482 5.13e-144 424
MS.gene042247.t1 MTR_4g066280 45.570 474 230 5 29 499 46 494 3.17e-143 422
MS.gene042247.t1 MTR_3g026400 45.745 470 247 5 33 500 17 480 6.17e-141 416
MS.gene042247.t1 MTR_2g043200 46.234 478 221 7 29 498 70 519 1.88e-137 408
MS.gene042247.t1 MTR_4g066630 48.804 418 209 4 29 443 44 459 1.04e-136 404
MS.gene042247.t1 MTR_4g083000 46.988 415 211 5 91 500 1 411 1.84e-133 394
MS.gene042247.t1 MTR_6g078450 47.857 420 212 6 29 444 41 457 3.17e-133 395
MS.gene042247.t1 MTR_6g078330 46.135 414 218 5 91 501 1 412 1.52e-132 392
MS.gene042247.t1 MTR_4g066630 49.608 383 188 4 29 408 44 424 3.40e-130 387
MS.gene042247.t1 MTR_3g028720 45.694 418 222 4 29 443 43 458 2.64e-128 382
MS.gene042247.t1 MTR_4g015420 42.245 490 245 8 21 498 62 525 6.32e-128 384
MS.gene042247.t1 MTR_4g015460 42.052 497 251 7 15 500 33 503 8.79e-128 383
MS.gene042247.t1 MTR_7g068910 39.468 489 280 5 18 503 25 500 2.55e-127 382
MS.gene042247.t1 MTR_4g016920 40.619 485 258 6 29 503 32 496 6.39e-127 381
MS.gene042247.t1 MTR_4g066580 48.042 383 194 4 29 408 44 424 9.02e-127 377
MS.gene042247.t1 MTR_7g068910 38.677 499 280 6 18 503 25 510 3.20e-124 374
MS.gene042247.t1 MTR_4g066240 47.301 389 200 4 23 408 35 421 6.68e-123 368
MS.gene042247.t1 MTR_3g028720 46.736 383 199 4 29 408 43 423 1.38e-122 367
MS.gene042247.t1 MTR_6g078330 44.776 402 215 6 103 501 15 412 7.07e-122 365
MS.gene042247.t1 MTR_5g068740 39.357 498 282 9 3 496 7 488 1.19e-121 367
MS.gene042247.t1 MTR_1g088610 38.966 503 276 9 3 496 7 487 5.74e-121 370
MS.gene042247.t1 MTR_1g088610 35.537 121 70 2 383 501 503 617 7.98e-16 80.9
MS.gene042247.t1 MTR_4g067040 47.978 371 188 4 29 396 39 407 4.19e-119 357
MS.gene042247.t1 MTR_4g066630 52.848 316 146 3 29 341 44 359 3.45e-115 346
MS.gene042247.t1 MTR_7g033860 39.535 473 269 8 29 497 53 512 2.96e-114 348
MS.gene042247.t1 MTR_4g067040 49.422 346 170 4 29 371 39 382 4.78e-114 343
MS.gene042247.t1 MTR_0110s0040 50.641 312 153 1 29 340 48 358 4.83e-114 344
MS.gene042247.t1 MTR_0108s0030 38.445 476 252 9 29 497 8 449 4.26e-112 341
MS.gene042247.t1 MTR_3g026400 50.629 318 155 2 23 339 36 352 2.21e-110 333
MS.gene042247.t1 MTR_6g078330 49.846 325 160 3 20 341 33 357 6.10e-109 330
MS.gene042247.t1 MTR_3g028720 46.821 346 179 4 29 371 43 386 1.16e-108 330
MS.gene042247.t1 MTR_4g066640 48.563 348 168 6 29 371 44 385 2.98e-108 328
MS.gene042247.t1 MTR_7g068900 38.824 425 239 7 85 503 16 425 5.95e-105 323
MS.gene042247.t1 MTR_4g016920 39.243 423 227 6 91 503 1 403 2.21e-104 320
MS.gene042247.t1 MTR_7g051510 36.907 485 268 10 29 502 80 537 7.07e-101 315
MS.gene042247.t1 MTR_1g088630 37.581 463 245 10 43 501 14 436 3.93e-99 308
MS.gene042247.t1 MTR_7g051610 35.892 482 267 11 29 497 80 532 2.78e-95 300
MS.gene042247.t1 MTR_7g051650 36.688 477 271 9 29 497 80 533 3.40e-94 297
MS.gene042247.t1 MTR_7g051650 36.268 477 269 9 29 497 80 529 7.49e-93 294
MS.gene042247.t1 MTR_7g056747 37.260 416 230 8 86 497 10 398 2.02e-84 268
MS.gene042247.t1 MTR_1g088630 41.765 340 168 6 3 340 7 318 4.96e-83 263
MS.gene042247.t1 MTR_0110s0020 53.363 223 103 1 29 251 48 269 1.13e-81 258
MS.gene042247.t1 MTR_1g088630 45.455 286 150 4 3 286 7 288 4.23e-78 248
MS.gene042247.t1 MTR_4g016920 45.312 256 138 2 91 345 1 255 3.48e-76 243
MS.gene042247.t1 MTR_4g122980 25.785 446 244 15 87 521 183 552 1.45e-30 126
MS.gene042247.t1 MTR_2g022730 45.679 81 42 1 374 452 24 104 1.11e-16 79.3
MS.gene042247.t1 MTR_8g038650 36.792 106 66 1 240 345 51 155 3.25e-16 76.6
MS.gene042247.t1 MTR_5g069350 33.125 160 55 6 355 498 66 189 5.72e-15 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042247.t1 AT1G61820 61.426 477 182 2 25 499 31 507 0.0 637
MS.gene042247.t1 AT1G61810 61.845 477 180 2 25 499 34 510 0.0 636
MS.gene042247.t1 AT4G21760 60.752 479 183 2 27 505 57 530 0.0 633
MS.gene042247.t1 AT1G61810 61.688 462 175 2 25 484 34 495 0.0 614
MS.gene042247.t1 AT4G21760 59.499 479 179 3 27 505 57 520 0.0 613
MS.gene042247.t1 AT1G61810 60.732 410 160 1 91 499 1 410 0.0 541
MS.gene042247.t1 AT1G61810 61.917 386 145 2 25 408 34 419 5.22e-179 511
MS.gene042247.t1 AT1G61820 59.239 368 149 1 133 499 1 368 3.87e-167 479
MS.gene042247.t1 AT1G26560 48.734 474 233 6 29 496 37 506 3.14e-160 466
MS.gene042247.t1 AT3G18080 46.862 478 236 5 29 499 44 510 1.14e-156 457
MS.gene042247.t1 AT5G54570 47.401 481 246 6 21 496 27 505 9.65e-152 445
MS.gene042247.t1 AT3G18070 45.094 479 243 6 29 500 35 500 5.45e-150 439
MS.gene042247.t1 AT2G44450 46.653 478 244 6 29 502 36 506 1.86e-149 438
MS.gene042247.t1 AT3G18070 44.885 479 238 6 29 500 35 494 6.46e-149 437
MS.gene042247.t1 AT2G44480 46.154 481 254 4 20 497 31 509 9.49e-149 437
MS.gene042247.t1 AT2G44480 46.154 481 254 4 20 497 62 540 2.88e-148 437
MS.gene042247.t1 AT5G44640 45.702 477 247 7 29 500 36 505 3.71e-147 432
MS.gene042247.t1 AT5G42260 46.695 469 238 7 29 492 36 497 4.31e-146 430
MS.gene042247.t1 AT3G60130 45.666 473 245 7 29 496 35 500 1.98e-144 426
MS.gene042247.t1 AT2G25630 45.073 477 233 8 29 500 35 487 4.46e-138 409
MS.gene042247.t1 AT5G36890 45.435 471 242 6 29 496 21 479 1.36e-137 407
MS.gene042247.t1 AT5G36890 45.435 471 242 6 29 496 21 479 1.41e-137 407
MS.gene042247.t1 AT3G60140 41.794 524 297 7 5 521 7 529 8.16e-137 409
MS.gene042247.t1 AT5G24550 43.125 480 268 5 24 498 32 511 8.05e-135 402
MS.gene042247.t1 AT4G27830 41.317 501 272 8 1 496 1 484 5.66e-134 399
MS.gene042247.t1 AT1G02850 43.100 471 249 7 29 496 31 485 9.31e-133 395
MS.gene042247.t1 AT4G27820 42.593 486 254 8 17 496 16 482 1.02e-132 395
MS.gene042247.t1 AT5G54570 46.838 427 222 4 21 444 27 451 1.79e-131 390
MS.gene042247.t1 AT1G61810 66.792 265 87 1 25 288 34 298 1.58e-130 383
MS.gene042247.t1 AT5G24540 43.333 480 267 5 24 498 32 511 4.80e-129 387
MS.gene042247.t1 AT2G44470 39.922 516 303 7 21 531 26 539 1.71e-128 387
MS.gene042247.t1 AT1G02850 41.296 494 248 8 29 496 31 508 5.39e-128 384
MS.gene042247.t1 AT2G44480 45.721 409 218 3 91 497 1 407 1.81e-127 379
MS.gene042247.t1 AT2G44460 42.659 504 279 8 21 519 26 524 2.20e-126 382
MS.gene042247.t1 AT1G02850 41.212 495 248 9 29 496 31 509 4.66e-126 379
MS.gene042247.t1 AT1G47600 42.116 482 263 8 21 498 42 511 1.13e-125 378
MS.gene042247.t1 AT1G47600 42.116 482 262 8 21 498 42 510 2.06e-125 377
MS.gene042247.t1 AT3G60130 44.819 415 218 6 86 496 41 448 1.16e-124 373
MS.gene042247.t1 AT4G22100 40.421 475 267 6 29 501 27 487 4.03e-124 374
MS.gene042247.t1 AT5G54570 45.610 410 217 5 91 496 1 408 6.47e-124 370
MS.gene042247.t1 AT1G51470 41.701 482 265 8 21 498 42 511 1.65e-123 372
MS.gene042247.t1 AT1G60090 39.400 500 278 9 2 496 3 482 7.15e-123 370
MS.gene042247.t1 AT1G60090 38.767 503 284 9 2 496 3 489 3.00e-122 369
MS.gene042247.t1 AT2G32860 41.250 480 273 8 29 501 99 576 2.03e-120 368
MS.gene042247.t1 AT1G02850 40.764 471 233 8 29 496 31 458 3.75e-120 362
MS.gene042247.t1 AT3G62750 39.419 482 266 9 17 496 16 473 3.04e-119 361
MS.gene042247.t1 AT2G32860 41.458 480 271 9 29 501 99 575 3.95e-119 364
MS.gene042247.t1 AT3G62750 39.419 482 266 9 17 496 42 499 8.63e-119 360
MS.gene042247.t1 AT3G60130 43.855 415 211 7 86 496 41 437 2.93e-118 357
MS.gene042247.t1 AT3G60120 39.918 486 285 7 18 498 8 491 8.23e-118 358
MS.gene042247.t1 AT2G44490 39.200 500 293 10 29 519 19 516 3.23e-117 358
MS.gene042247.t1 AT5G26000 41.532 496 267 10 24 507 38 522 4.65e-117 357
MS.gene042247.t1 AT3G21370 40.947 486 273 11 21 496 30 511 2.04e-116 354
MS.gene042247.t1 AT1G45191 36.926 501 291 8 4 501 10 488 1.36e-115 352
MS.gene042247.t1 AT5G25980 41.803 488 264 13 19 496 45 522 2.35e-115 352
MS.gene042247.t1 AT1G45191 36.978 503 294 7 4 501 10 494 1.86e-114 349
MS.gene042247.t1 AT1G45191 37.200 500 302 5 4 501 10 499 1.60e-113 347
MS.gene042247.t1 AT1G45191 36.811 508 305 5 4 501 10 511 3.06e-112 344
MS.gene042247.t1 AT3G62740 36.821 497 282 10 7 496 7 478 1.34e-111 341
MS.gene042247.t1 AT1G66280 39.832 477 275 8 29 498 41 512 1.09e-109 337
MS.gene042247.t1 AT1G66270 39.413 477 277 8 29 498 41 512 1.09e-109 337
MS.gene042247.t1 AT3G09260 38.716 483 289 7 21 498 32 512 3.09e-109 336
MS.gene042247.t1 AT3G18070 48.397 312 158 2 29 340 35 343 6.67e-109 330
MS.gene042247.t1 AT4G27830 40.146 411 226 6 91 496 1 396 7.88e-109 331
MS.gene042247.t1 AT1G75940 38.900 491 262 11 29 496 42 517 4.57e-108 333
MS.gene042247.t1 AT5G28510 38.650 489 286 8 21 498 36 521 2.28e-107 331
MS.gene042247.t1 AT4G27820 41.019 412 222 7 91 496 1 397 2.98e-107 327
MS.gene042247.t1 AT4G27820 41.019 412 222 7 91 496 1 397 2.98e-107 327
MS.gene042247.t1 AT1G52400 39.220 487 280 12 20 496 35 515 8.67e-107 330
MS.gene042247.t1 AT1G52400 39.220 487 280 12 20 496 35 515 8.67e-107 330
MS.gene042247.t1 AT1G66270 39.203 477 276 10 29 498 41 510 2.50e-106 328
MS.gene042247.t1 AT2G44470 39.437 426 254 4 21 443 26 450 2.78e-106 326
MS.gene042247.t1 AT5G24540 41.608 423 244 3 24 443 32 454 7.61e-106 325
MS.gene042247.t1 AT3G60140 41.032 407 236 4 5 408 7 412 1.35e-105 324
MS.gene042247.t1 AT1G02850 41.584 404 202 5 29 408 31 424 1.78e-104 320
MS.gene042247.t1 AT3G03640 36.210 533 315 11 1 513 1 528 2.88e-104 323
MS.gene042247.t1 AT2G44480 49.841 315 155 3 20 332 62 375 3.25e-102 313
MS.gene042247.t1 AT2G44450 43.094 362 197 4 143 502 1 355 2.70e-101 310
MS.gene042247.t1 AT5G25980 40.878 433 239 11 19 444 45 467 2.34e-98 306
MS.gene042247.t1 AT5G25980 40.878 433 239 11 19 444 45 467 2.34e-98 306
MS.gene042247.t1 AT5G26000 40.706 425 232 8 29 444 43 456 3.23e-97 303
MS.gene042247.t1 AT3G62750 37.674 430 244 8 17 446 42 447 3.91e-97 303
MS.gene042247.t1 AT3G62750 37.990 408 231 8 91 496 1 388 3.24e-96 298
MS.gene042247.t1 AT3G62750 37.990 408 231 8 91 496 1 388 3.24e-96 298
MS.gene042247.t1 AT1G60270 43.544 333 182 3 3 333 1 329 4.33e-95 295
MS.gene042247.t1 AT2G44470 45.171 321 173 3 21 338 26 346 2.20e-93 291
MS.gene042247.t1 AT3G18070 42.059 340 180 4 168 500 94 423 8.07e-93 290
MS.gene042247.t1 AT3G18070 55.357 56 25 0 29 84 35 90 2.05e-12 69.7
MS.gene042247.t1 AT3G62740 37.838 407 229 8 7 408 7 394 3.66e-92 288
MS.gene042247.t1 AT3G62750 42.901 324 172 4 17 340 16 326 1.03e-90 283
MS.gene042247.t1 AT3G62750 42.901 324 172 4 17 340 16 326 1.03e-90 283
MS.gene042247.t1 AT3G62750 42.901 324 172 4 17 340 42 352 1.79e-89 282
MS.gene042247.t1 AT1G45191 40.909 330 189 3 4 333 10 333 3.09e-89 280
MS.gene042247.t1 AT1G45191 40.909 330 189 3 4 333 10 333 3.78e-89 280
MS.gene042247.t1 AT1G52400 38.426 432 252 10 20 443 35 460 1.73e-88 280
MS.gene042247.t1 AT3G18070 41.875 320 169 4 188 500 2 311 8.67e-87 271
MS.gene042247.t1 AT2G44460 40.992 383 217 7 141 519 1 378 1.33e-83 266
MS.gene042247.t1 AT5G48375 36.364 418 199 9 52 462 54 411 5.84e-79 254
MS.gene042247.t1 AT2G44480 40.864 301 175 2 198 497 5 303 8.94e-78 247
MS.gene042247.t1 AT1G51490 43.408 311 160 9 32 338 28 326 4.43e-72 236
MS.gene042247.t1 AT1G60270 43.210 243 137 1 91 333 1 242 1.84e-70 228
MS.gene042247.t1 AT3G18070 41.296 247 132 2 168 408 94 333 3.32e-63 211
MS.gene042247.t1 AT3G18070 55.357 56 25 0 29 84 35 90 1.00e-12 70.1
MS.gene042247.t1 AT3G18070 40.969 227 121 2 188 408 2 221 4.69e-57 191
MS.gene042247.t1 AT5G16580 33.446 296 177 7 162 454 21 299 9.83e-47 165
MS.gene042247.t1 AT3G06510 25.935 401 218 14 86 477 159 489 1.98e-27 117
MS.gene042247.t1 AT3G06510 25.243 412 241 12 86 477 159 523 5.91e-27 115

Find 123 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTTCCTTGGTGTTATT+TGG 0.081941 8.2:+3300656 None:intergenic
TTTGTCGTTACTGGAAAATT+TGG 0.210997 8.2:+3301161 None:intergenic
TGTCGCTGCTCCCGCAAATT+TGG 0.214872 8.2:-3301931 MS.gene042247:CDS
TACCAACTAGCTGACAATTT+TGG 0.228855 8.2:+3300343 None:intergenic
AAAATCACCTCCATCTCTTT+TGG 0.232607 8.2:+3301198 None:intergenic
CTCGTATCCGTGTCATGTTC+TGG 0.251671 8.2:+3300280 None:intergenic
GACAACTTTGAGTGGCTATT+TGG 0.252310 8.2:-3300421 MS.gene042247:CDS
TTTCCATCCAACTTTCTCTT+TGG 0.260893 8.2:-3303169 MS.gene042247:CDS
ACAGGAGGATGTAGATTTAA+TGG 0.268522 8.2:-3302591 MS.gene042247:intron
CCAAGTGAAACTTGGAGATT+TGG 0.292516 8.2:+3301979 None:intergenic
CACTGCTTCTTCTGCCTACC+AGG 0.320999 8.2:-3303146 MS.gene042247:intron
GACTGGTCACTTAGATAATC+TGG 0.322076 8.2:-3300603 MS.gene042247:CDS
CTGGTATTGACTACTATAAT+AGG 0.334064 8.2:-3302374 MS.gene042247:CDS
GCTGAGCAGGGAGGACAAAT+TGG 0.348657 8.2:-3301624 MS.gene042247:CDS
ATGTTCTGGATTACTGCTTT+CGG 0.369526 8.2:+3300294 None:intergenic
CTGTGAGCAGAAGGTAGATT+TGG 0.371053 8.2:-3302411 MS.gene042247:intron
GTTTCACTTGGTTACCGAAA+TGG 0.373316 8.2:-3301967 MS.gene042247:CDS
TTATTCACTACCTATGCTCT+TGG 0.374400 8.2:-3303229 MS.gene042247:CDS
TTGTCGTTACTGGAAAATTT+GGG 0.378693 8.2:+3301162 None:intergenic
CGCTACAATTTCCAAATTTG+CGG 0.390612 8.2:+3301920 None:intergenic
ACCAGTTGCTCACTGTGATT+GGG 0.394203 8.2:+3302158 None:intergenic
AACCAGTTGCTCACTGTGAT+TGG 0.401532 8.2:+3302157 None:intergenic
GCTACAATTTCCAAATTTGC+GGG 0.406017 8.2:+3301921 None:intergenic
TGATCAATATCATCGGTATC+TGG 0.408911 8.2:-3302822 MS.gene042247:intron
CATGTGATAGGATAATATTA+TGG 0.409137 8.2:+3301863 None:intergenic
CAAGAACTTGACGACAGATA+TGG 0.410450 8.2:-3302195 MS.gene042247:CDS
TCTATAATAATGCAGGATCT+TGG 0.411513 8.2:-3301244 MS.gene042247:intron
CAACTGCAGCTGCATGTGAT+AGG 0.416110 8.2:+3301851 None:intergenic
ATGACACGGATACGAGAAAC+TGG 0.424883 8.2:-3300273 MS.gene042247:CDS
ATGGTGGGTGTTTACCATTT+CGG 0.426254 8.2:+3301953 None:intergenic
AATCGAGGACTCGATTTCAT+TGG 0.429047 8.2:-3301129 MS.gene042247:CDS
GGGTACTTGCCGAAGAAGAT+TGG 0.431182 8.2:+3301219 None:intergenic
AATATTATGGGCTGCTACAA+AGG 0.431696 8.2:+3301876 None:intergenic
TTCGGTAACCAAGTGAAACT+TGG 0.437487 8.2:+3301971 None:intergenic
GCATTGTCGTACACGTTGAC+TGG 0.438002 8.2:-3301602 MS.gene042247:CDS
GTTTGAACCGTTTAGCAATT+CGG 0.441412 8.2:-3301580 MS.gene042247:CDS
ATATCATCAATGTGTGAGTC+TGG 0.444978 8.2:-3301069 MS.gene042247:CDS
ATCTTGGATCCAATCTTCTT+CGG 0.447074 8.2:-3301228 MS.gene042247:CDS
ACGCTGCATCCAGGCTGAGC+AGG 0.461536 8.2:-3301637 MS.gene042247:intron
CCAATGTTCATTACTGAGAA+TGG 0.464727 8.2:-3300791 MS.gene042247:intron
ATCATTAAGACATTCTTCCT+TGG 0.465143 8.2:+3300646 None:intergenic
CTCAATCATTCAGCATGAAC+TGG 0.467873 8.2:-3301530 MS.gene042247:intron
ATGTGTAGACTCCATTTGAT+TGG 0.470062 8.2:-3300880 MS.gene042247:intron
CCAAATCTCCAAGTTTCACT+TGG 0.472557 8.2:-3301979 MS.gene042247:CDS
GTGCTTACAAGCTCTCCAAT+GGG 0.474734 8.2:+3300988 None:intergenic
ATTGGCATCAATCATTATGC+AGG 0.475815 8.2:-3301111 MS.gene042247:CDS
AAGAGAAAGTTGGATGGAAA+AGG 0.476077 8.2:+3303172 None:intergenic
ATATTATGGGCTGCTACAAA+GGG 0.484402 8.2:+3301877 None:intergenic
ATGTGATAGGATAATATTAT+GGG 0.491989 8.2:+3301864 None:intergenic
TAAACATTGAGCCAATCAAA+TGG 0.504017 8.2:+3300869 None:intergenic
TTTGGAGAAGTCAACTTGGC+TGG 0.504319 8.2:-3302393 MS.gene042247:CDS
ATCTGTCGTCAAGTTCTTGA+GGG 0.506725 8.2:+3302199 None:intergenic
AACTTAATATTATAGTATGA+AGG 0.507201 8.2:-3303055 MS.gene042247:intron
ATGTGAGAGGATACTTTGCC+TGG 0.508878 8.2:-3300453 MS.gene042247:CDS
ATGGCAAAGGATTGAGTGAC+TGG 0.510545 8.2:-3303018 MS.gene042247:CDS
TCAGCTACCGAATTGCTAAA+CGG 0.512445 8.2:+3301573 None:intergenic
ACTGGTCACTTAGATAATCT+GGG 0.513194 8.2:-3300602 MS.gene042247:CDS
CTATCAAAGTCAACAGCTAC+CGG 0.515390 8.2:-3302565 MS.gene042247:CDS
TTGGTGACAGAGTTAAGTAC+TGG 0.515563 8.2:-3302017 MS.gene042247:CDS
AAACTGGAACAAACAATTCA+AGG 0.521421 8.2:-3300257 MS.gene042247:CDS
AGCAGTGCCAAAGAGAAAGT+TGG 0.523449 8.2:+3303162 None:intergenic
TCTACTACCCTCTGAGCCTA+CGG 0.523808 8.2:+3300219 None:intergenic
CGCTGCATCCAGGCTGAGCA+GGG 0.527593 8.2:-3301636 MS.gene042247:intron
AAGTGAAACAATGTAACAAA+CGG 0.528983 8.2:+3302228 None:intergenic
CTCCAAAATTGTCAGCTAGT+TGG 0.530460 8.2:-3300345 MS.gene042247:CDS
TTGCCCACGAAATTGAAAAC+CGG 0.531948 8.2:+3302546 None:intergenic
GAGATTTGGTTCATTGAATG+TGG 0.532609 8.2:+3301993 None:intergenic
CTGTTACCTATGTGAAGGAC+AGG 0.533086 8.2:-3300826 MS.gene042247:CDS
CTTAGAACCGTAGGCTCAGA+GGG 0.534583 8.2:-3300226 MS.gene042247:CDS
CCTTGCTTGACAACTTTGAG+TGG 0.544158 8.2:-3300429 MS.gene042247:CDS
ATTGTACCTGTCCTTCACAT+AGG 0.545267 8.2:+3300820 None:intergenic
CCATTCTCAGTAATGAACAT+TGG 0.554982 8.2:+3300791 None:intergenic
AGTGCTTACAAGCTCTCCAA+TGG 0.557979 8.2:+3300987 None:intergenic
GTATAAACCCTCTGTTGTGG+AGG 0.558557 8.2:+3301038 None:intergenic
GAAACAATGTAACAAACGGT+TGG 0.558611 8.2:+3302232 None:intergenic
AAAAGAATAAACTACATGAC+TGG 0.562119 8.2:-3300620 MS.gene042247:CDS
TGAGAATGCTCCAAGAGCAT+AGG 0.565601 8.2:+3303219 None:intergenic
AAAGTTGTCAAGCAAGGACC+AGG 0.567557 8.2:+3300435 None:intergenic
GCAAATATGCTTAGAACCGT+AGG 0.568060 8.2:-3300235 MS.gene042247:CDS
AAATTTGGAAATTGTAGCGA+GGG 0.575693 8.2:-3301916 MS.gene042247:CDS
AGGGAAGGAGCAGATGTGAG+AGG 0.576362 8.2:-3300466 MS.gene042247:CDS
TTGACGACAGATATGGAGGT+TGG 0.586833 8.2:-3302188 MS.gene042247:CDS
TATCTGTCGTCAAGTTCTTG+AGG 0.589618 8.2:+3302198 None:intergenic
ATCGACGCTTTGCTACTCAA+AGG 0.594955 8.2:-3302348 MS.gene042247:intron
TGCGGGAGCAGCGACATGGT+GGG 0.596391 8.2:+3301938 None:intergenic
CAAGGAACCTCCACAACAGA+GGG 0.596892 8.2:-3301045 MS.gene042247:CDS
CGGCAAGTACCCAAAAGAGA+TGG 0.598644 8.2:-3301208 MS.gene042247:CDS
GAAAACTGTTACCTATGTGA+AGG 0.598748 8.2:-3300831 MS.gene042247:CDS
TTGCGGGAGCAGCGACATGG+TGG 0.599395 8.2:+3301937 None:intergenic
CCAGTTGCTCACTGTGATTG+GGG 0.602755 8.2:+3302159 None:intergenic
TAGATTTGGAGAAGTCAACT+TGG 0.607687 8.2:-3302397 MS.gene042247:CDS
TTGGTATAAACCCTCTGTTG+TGG 0.608835 8.2:+3301035 None:intergenic
TGGCTCAATGTTTACCCACA+AGG 0.615458 8.2:-3300860 MS.gene042247:CDS
GTGCCAAAGAGAAAGTTGGA+TGG 0.621854 8.2:+3303166 None:intergenic
TAGCGAGGGAGATTCAGAGA+AGG 0.625947 8.2:-3301902 MS.gene042247:CDS
AGTACTGATGATGGAAGTAA+CGG 0.629271 8.2:-3302857 MS.gene042247:CDS
GCTTAGAACCGTAGGCTCAG+AGG 0.630554 8.2:-3300227 MS.gene042247:CDS
CAGTAATCCAGAACATGACA+CGG 0.631088 8.2:-3300287 MS.gene042247:CDS
CCACTCAAAGTTGTCAAGCA+AGG 0.631883 8.2:+3300429 None:intergenic
TTACTGTTGATCAATATCAT+CGG 0.633059 8.2:-3302829 MS.gene042247:CDS
AATTTGCGGGAGCAGCGACA+TGG 0.635985 8.2:+3301934 None:intergenic
CTGGTCACTTAGATAATCTG+GGG 0.638340 8.2:-3300601 MS.gene042247:CDS
TCAATGTGTGAGTCTGGGCA+AGG 0.639903 8.2:-3301063 MS.gene042247:CDS
GTACTTAACTCTGTCACCAA+AGG 0.643194 8.2:+3302020 None:intergenic
TATCATCAATGTGTGAGTCT+GGG 0.645232 8.2:-3301068 MS.gene042247:CDS
CAAGTACCCAAAAGAGATGG+AGG 0.647849 8.2:-3301205 MS.gene042247:CDS
GAAGGTGCTTACTTGAGTGA+TGG 0.651168 8.2:-3303037 MS.gene042247:CDS
CAAATTTGGAAATTGTAGCG+AGG 0.653756 8.2:-3301917 MS.gene042247:CDS
GAACTTGACGACAGATATGG+AGG 0.657453 8.2:-3302192 MS.gene042247:CDS
AGAAGGTAAAGAAACCTGGT+AGG 0.658277 8.2:+3303132 None:intergenic
TGGAGCATTCTCATTCTCAG+AGG 0.661583 8.2:-3303209 MS.gene042247:CDS
GCTTACTTGAGTGATGGCAA+AGG 0.664601 8.2:-3303031 MS.gene042247:CDS
TGCATCCAGGCTGAGCAGGG+AGG 0.666984 8.2:-3301633 MS.gene042247:intron
TATTTATAGAAACAAATACC+AGG 0.672534 8.2:-3301827 MS.gene042247:intron
GATGTCTTCACTCATAAACC+AGG 0.676578 8.2:-3302995 MS.gene042247:intron
TCTGCGTAGCAAAATCAACA+TGG 0.676856 8.2:+3300374 None:intergenic
GACAAAGCAAAACTGAATCG+AGG 0.686534 8.2:-3301144 MS.gene042247:CDS
GCAAGGAACCTCCACAACAG+AGG 0.691222 8.2:-3301046 MS.gene042247:CDS
TGGCAAAGGATTGAGTGACT+GGG 0.697528 8.2:-3303017 MS.gene042247:CDS
TGCTTACAAGCTCTCCAATG+GGG 0.708162 8.2:+3300989 None:intergenic
TATTATGGGCTGCTACAAAG+GGG 0.721105 8.2:+3301878 None:intergenic
GTATGATCCAAATAACACCA+AGG 0.728521 8.2:-3300663 MS.gene042247:CDS
TGGTCACTTAGATAATCTGG+GGG 0.736530 8.2:-3300600 MS.gene042247:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAAAATGTAACTAA+TGG + chr8.2:3302510-3302529 None:intergenic 10.0%
!!! ATTAAAATAATGAAGATTTT+AGG + chr8.2:3300360-3300379 None:intergenic 10.0%
!!! TAATAACATATATTTTTTTC+AGG - chr8.2:3300573-3300592 MS.gene042247:intron 10.0%
!!! TTCATTATTTTAATTAATCA+TGG - chr8.2:3300366-3300385 MS.gene042247:CDS 10.0%
!! AACTTAATATTATAGTATGA+AGG - chr8.2:3300399-3300418 MS.gene042247:CDS 15.0%
!! ACATTTAGACCATATATAAT+GGG + chr8.2:3300828-3300847 None:intergenic 20.0%
!! ATGTGATAGGATAATATTAT+GGG + chr8.2:3301593-3301612 None:intergenic 20.0%
!! GTAATTAGTTAAAGTAGATA+AGG + chr8.2:3300804-3300823 None:intergenic 20.0%
!! TATTTATAGAAACAAATACC+AGG - chr8.2:3301627-3301646 MS.gene042247:CDS 20.0%
!! TGTCAATTTGTAATAAAAAC+AGG - chr8.2:3300845-3300864 MS.gene042247:CDS 20.0%
!!! AAAATCCAATTTAGTTTAAG+AGG - chr8.2:3300715-3300734 MS.gene042247:intron 20.0%
!!! CACATCATAATACTATTTTT+TGG - chr8.2:3300657-3300676 MS.gene042247:CDS 20.0%
!!! TAAATTGGATTTTGATTATG+AGG + chr8.2:3300708-3300727 None:intergenic 20.0%
! AAAAGAATAAACTACATGAC+TGG - chr8.2:3302834-3302853 MS.gene042247:CDS 25.0%
! AACAATACAACTTTCAAGAT+TGG + chr8.2:3302554-3302573 None:intergenic 25.0%
! AAGTGAAACAATGTAACAAA+CGG + chr8.2:3301229-3301248 None:intergenic 25.0%
! ACAATACAACTTTCAAGATT+GGG + chr8.2:3302553-3302572 None:intergenic 25.0%
! ACATCAAAGTTATCGATATA+TGG - chr8.2:3302912-3302931 MS.gene042247:intron 25.0%
! AGGTATTCAAGTATGAAAAA+TGG + chr8.2:3300519-3300538 None:intergenic 25.0%
! CAATTTGTAATAAAAACAGG+AGG - chr8.2:3300848-3300867 MS.gene042247:CDS 25.0%
! CATGTGATAGGATAATATTA+TGG + chr8.2:3301594-3301613 None:intergenic 25.0%
! CGATAACTTTGATGTTTATT+CGG + chr8.2:3302907-3302926 None:intergenic 25.0%
! CTAATTACACCCATTATATA+TGG - chr8.2:3300816-3300835 MS.gene042247:CDS 25.0%
! GACATTTAGACCATATATAA+TGG + chr8.2:3300829-3300848 None:intergenic 25.0%
! GTCAAAATACATTATAATGC+TGG + chr8.2:3301368-3301387 None:intergenic 25.0%
! TAACTAATGGAATCATCAAA+AGG + chr8.2:3302497-3302516 None:intergenic 25.0%
! TGGTTGAAATTCAATGAAAA+TGG + chr8.2:3302716-3302735 None:intergenic 25.0%
! TGTAACCTCTTAAACTAAAT+TGG + chr8.2:3300723-3300742 None:intergenic 25.0%
! TTACTGTTGATCAATATCAT+CGG - chr8.2:3300625-3300644 MS.gene042247:CDS 25.0%
!!! AATGTATTTTGACATGTACA+GGG - chr8.2:3301375-3301394 MS.gene042247:intron 25.0%
!!! GACATATGTTTTAAATCCTT+TGG - chr8.2:3301418-3301437 MS.gene042247:intron 25.0%
!!! TAATGTATTTTGACATGTAC+AGG - chr8.2:3301374-3301393 MS.gene042247:intron 25.0%
!!! TCACTTTTGACTTCATATTT+TGG - chr8.2:3302067-3302086 MS.gene042247:intron 25.0%
!!! TTTGGTTTTACAGTGAAATT+TGG - chr8.2:3303051-3303070 MS.gene042247:intron 25.0%
AAACTGGAACAAACAATTCA+AGG - chr8.2:3303197-3303216 MS.gene042247:CDS 30.0%
AAATAGCTGAGTATTTGTGT+TGG - chr8.2:3302953-3302972 MS.gene042247:intron 30.0%
AAATTTGGAAATTGTAGCGA+GGG - chr8.2:3301538-3301557 MS.gene042247:CDS 30.0%
AATGGTTCAAATCATGTGAT+TGG - chr8.2:3302133-3302152 MS.gene042247:intron 30.0%
AGACTGAAATCTTGATCAAA+TGG - chr8.2:3302115-3302134 MS.gene042247:intron 30.0%
ATCATTAAGACATTCTTCCT+TGG + chr8.2:3302811-3302830 None:intergenic 30.0%
ATTATGTAATTACGCAACTC+AGG + chr8.2:3300557-3300576 None:intergenic 30.0%
GGGTAGTAGAATAAAAAACT+TGG - chr8.2:3303248-3303267 MS.gene042247:CDS 30.0%
GTTTGACTCTATAATAATGC+AGG - chr8.2:3302203-3302222 MS.gene042247:CDS 30.0%
TAAACATTGAGCCAATCAAA+TGG + chr8.2:3302588-3302607 None:intergenic 30.0%
TACCATGCAAAAGTTTGATT+TGG - chr8.2:3301990-3302009 MS.gene042247:CDS 30.0%
TACTCTGACTTTAGTAAATC+AGG + chr8.2:3302093-3302112 None:intergenic 30.0%
TCTATAATAATGCAGGATCT+TGG - chr8.2:3302210-3302229 MS.gene042247:CDS 30.0%
TCTGTGTAAGAGACTAATAA+TGG + chr8.2:3302736-3302755 None:intergenic 30.0%
TTAGGAGATAAACATGAAGA+AGG + chr8.2:3300342-3300361 None:intergenic 30.0%
TTATCGATATATGGTTTACC+TGG - chr8.2:3302921-3302940 MS.gene042247:intron 30.0%
TTGTCGTTACTGGAAAATTT+GGG + chr8.2:3302295-3302314 None:intergenic 30.0%
TTTGTCGTTACTGGAAAATT+TGG + chr8.2:3302296-3302315 None:intergenic 30.0%
! ATCCAAATCAAACTTTTGCA+TGG + chr8.2:3301995-3302014 None:intergenic 30.0%
! GGTCTTTTTACTATATTACC+TGG + chr8.2:3301648-3301667 None:intergenic 30.0%
! TATTGACACAACACTTGTTA+AGG + chr8.2:3300747-3300766 None:intergenic 30.0%
! TGATGTTTATTCGGTTTTTG+TGG + chr8.2:3302898-3302917 None:intergenic 30.0%
!! CTGGTATTGACTACTATAAT+AGG - chr8.2:3301080-3301099 MS.gene042247:CDS 30.0%
!! TCTATCCGAATGTTTTTGTA+GGG - chr8.2:3302757-3302776 MS.gene042247:intron 30.0%
AAGAGAAAGTTGGATGGAAA+AGG + chr8.2:3300285-3300304 None:intergenic 35.0%
ACAGGAGGATGTAGATTTAA+TGG - chr8.2:3300863-3300882 MS.gene042247:CDS 35.0%
ACTGGTCACTTAGATAATCT+GGG - chr8.2:3302852-3302871 MS.gene042247:CDS 35.0%
ATATCATCAATGTGTGAGTC+TGG - chr8.2:3302385-3302404 MS.gene042247:CDS 35.0%
ATCTTGGATCCAATCTTCTT+CGG - chr8.2:3302226-3302245 MS.gene042247:CDS 35.0%
ATGAAGAAGGTAAAGAAACC+TGG + chr8.2:3300329-3300348 None:intergenic 35.0%
ATGCTGGTAAAGTATGACAA+AGG + chr8.2:3301352-3301371 None:intergenic 35.0%
ATGTGTAGACTCCATTTGAT+TGG - chr8.2:3302574-3302593 MS.gene042247:CDS 35.0%
ATTGGCATCAATCATTATGC+AGG - chr8.2:3302343-3302362 MS.gene042247:intron 35.0%
CAAATTTGGAAATTGTAGCG+AGG - chr8.2:3301537-3301556 MS.gene042247:CDS 35.0%
CAACAAACTGCACAAAAAGA+TGG - chr8.2:3302439-3302458 MS.gene042247:intron 35.0%
CCAATGTTCATTACTGAGAA+TGG - chr8.2:3302663-3302682 MS.gene042247:intron 35.0%
CCATACCCTACAAAAACATT+CGG + chr8.2:3302765-3302784 None:intergenic 35.0%
CCATTCTCAGTAATGAACAT+TGG + chr8.2:3302666-3302685 None:intergenic 35.0%
CGCTACAATTTCCAAATTTG+CGG + chr8.2:3301537-3301556 None:intergenic 35.0%
GAAAACTGTTACCTATGTGA+AGG - chr8.2:3302623-3302642 MS.gene042247:intron 35.0%
GAAACAATGTAACAAACGGT+TGG + chr8.2:3301225-3301244 None:intergenic 35.0%
GAAATGTTGTTATCATGACC+TGG + chr8.2:3300480-3300499 None:intergenic 35.0%
GCTACAATTTCCAAATTTGC+GGG + chr8.2:3301536-3301555 None:intergenic 35.0%
GTATGATCCAAATAACACCA+AGG - chr8.2:3302791-3302810 MS.gene042247:intron 35.0%
GTTAAATCAAGATGCGTACT+TGG + chr8.2:3300942-3300961 None:intergenic 35.0%
GTTTGAACCGTTTAGCAATT+CGG - chr8.2:3301874-3301893 MS.gene042247:CDS 35.0%
TACAACTTTCAAGATTGGGT+TGG + chr8.2:3302549-3302568 None:intergenic 35.0%
TAGAGTCAAACTTCATGATC+AGG + chr8.2:3302194-3302213 None:intergenic 35.0%
TAGATTTGGAGAAGTCAACT+TGG - chr8.2:3301057-3301076 MS.gene042247:CDS 35.0%
TATCATCAATGTGTGAGTCT+GGG - chr8.2:3302386-3302405 MS.gene042247:CDS 35.0%
TCAGGTGATTGAATGAATGA+AGG + chr8.2:3300539-3300558 None:intergenic 35.0%
TGATCAATATCATCGGTATC+TGG - chr8.2:3300632-3300651 MS.gene042247:CDS 35.0%
TTATTCACTACCTATGCTCT+TGG - chr8.2:3300225-3300244 MS.gene042247:CDS 35.0%
TTTCCATCCAACTTTCTCTT+TGG - chr8.2:3300285-3300304 MS.gene042247:CDS 35.0%
! AAAATCACCTCCATCTCTTT+TGG + chr8.2:3302259-3302278 None:intergenic 35.0%
! AAAGAGATGGAGGTGATTTT+AGG - chr8.2:3302259-3302278 MS.gene042247:intron 35.0%
! AAATCACCTCCATCTCTTTT+GGG + chr8.2:3302258-3302277 None:intergenic 35.0%
! AATATTATGGGCTGCTACAA+AGG + chr8.2:3301581-3301600 None:intergenic 35.0%
! ATATTATGGGCTGCTACAAA+GGG + chr8.2:3301580-3301599 None:intergenic 35.0%
! ATGTTCTGGATTACTGCTTT+CGG + chr8.2:3303163-3303182 None:intergenic 35.0%
! CATCTTTTTGTGCAGTTTGT+TGG + chr8.2:3302441-3302460 None:intergenic 35.0%
! GAGATTTGGTTCATTGAATG+TGG + chr8.2:3301464-3301483 None:intergenic 35.0%
! TACCAACTAGCTGACAATTT+TGG + chr8.2:3303114-3303133 None:intergenic 35.0%
!! AGTACTGATGATGGAAGTAA+CGG - chr8.2:3300597-3300616 MS.gene042247:CDS 35.0%
!! CATTCTTCCTTGGTGTTATT+TGG + chr8.2:3302801-3302820 None:intergenic 35.0%
!! CTCTATCCGAATGTTTTTGT+AGG - chr8.2:3302756-3302775 MS.gene042247:intron 35.0%
!! GAGTATTTGTGTTGGTTATG+TGG - chr8.2:3302961-3302980 MS.gene042247:intron 35.0%
!!! TTTTCAGGTAGTACTGATGA+TGG - chr8.2:3300588-3300607 MS.gene042247:intron 35.0%
AACTTTGTAGCAGCATGCTA+AGG - chr8.2:3301672-3301691 MS.gene042247:intron 40.0%
AATCGAGGACTCGATTTCAT+TGG - chr8.2:3302325-3302344 MS.gene042247:intron 40.0%
AGAAGGTAAAGAAACCTGGT+AGG + chr8.2:3300325-3300344 None:intergenic 40.0%
AGTCAAACTTCATGATCAGG+TGG + chr8.2:3302191-3302210 None:intergenic 40.0%
ATCATTCAGCATGAACTGGT+AGG - chr8.2:3301928-3301947 MS.gene042247:CDS 40.0%
ATCTGTCGTCAAGTTCTTGA+GGG + chr8.2:3301258-3301277 None:intergenic 40.0%
ATTGTACCTGTCCTTCACAT+AGG + chr8.2:3302637-3302656 None:intergenic 40.0%
CAAGAACTTGACGACAGATA+TGG - chr8.2:3301259-3301278 MS.gene042247:intron 40.0%
CAGTAATCCAGAACATGACA+CGG - chr8.2:3303167-3303186 MS.gene042247:CDS 40.0%
CCAAATCTCCAAGTTTCACT+TGG - chr8.2:3301475-3301494 MS.gene042247:intron 40.0%
CCAAGTGAAACTTGGAGATT+TGG + chr8.2:3301478-3301497 None:intergenic 40.0%
CTATCAAAGTCAACAGCTAC+CGG - chr8.2:3300889-3300908 MS.gene042247:intron 40.0%
CTCAATCATTCAGCATGAAC+TGG - chr8.2:3301924-3301943 MS.gene042247:CDS 40.0%
CTCCAAAATTGTCAGCTAGT+TGG - chr8.2:3303109-3303128 MS.gene042247:intron 40.0%
CTGCAAGTATACAATTCGCA+GGG - chr8.2:3301197-3301216 MS.gene042247:CDS 40.0%
CTGGTCACTTAGATAATCTG+GGG - chr8.2:3302853-3302872 MS.gene042247:CDS 40.0%
GACAAAGCAAAACTGAATCG+AGG - chr8.2:3302310-3302329 MS.gene042247:intron 40.0%
GACAACTTTGAGTGGCTATT+TGG - chr8.2:3303033-3303052 MS.gene042247:CDS 40.0%
GATGTCTTCACTCATAAACC+AGG - chr8.2:3300459-3300478 MS.gene042247:CDS 40.0%
GCAAATATGCTTAGAACCGT+AGG - chr8.2:3303219-3303238 MS.gene042247:CDS 40.0%
GTACTTAACTCTGTCACCAA+AGG + chr8.2:3301437-3301456 None:intergenic 40.0%
GTGAATTGACTAGAACACAC+AGG + chr8.2:3301005-3301024 None:intergenic 40.0%
GTTTCACTTGGTTACCGAAA+TGG - chr8.2:3301487-3301506 MS.gene042247:intron 40.0%
TATCTGTCGTCAAGTTCTTG+AGG + chr8.2:3301259-3301278 None:intergenic 40.0%
TCAGCTACCGAATTGCTAAA+CGG + chr8.2:3301884-3301903 None:intergenic 40.0%
TCCTCATTGTTCAATGTCCA+TGG - chr8.2:3301954-3301973 MS.gene042247:CDS 40.0%
TCTGCGTAGCAAAATCAACA+TGG + chr8.2:3303083-3303102 None:intergenic 40.0%
TGAGCTGTTCAATCTTGATC+TGG + chr8.2:3302050-3302069 None:intergenic 40.0%
TGGTCACTTAGATAATCTGG+GGG - chr8.2:3302854-3302873 MS.gene042247:CDS 40.0%
TTCGGTAACCAAGTGAAACT+TGG + chr8.2:3301486-3301505 None:intergenic 40.0%
TTGCCCACGAAATTGAAAAC+CGG + chr8.2:3300911-3300930 None:intergenic 40.0%
TTGGTATAAACCCTCTGTTG+TGG + chr8.2:3302422-3302441 None:intergenic 40.0%
! ACAGTTTTCTTCATGCCTTG+TGG + chr8.2:3302612-3302631 None:intergenic 40.0%
! CAGTTTTCTTCATGCCTTGT+GGG + chr8.2:3302611-3302630 None:intergenic 40.0%
! CAGTTTTGCTTTGTCGTTAC+TGG + chr8.2:3302305-3302324 None:intergenic 40.0%
! GACTGGTCACTTAGATAATC+TGG - chr8.2:3302851-3302870 MS.gene042247:CDS 40.0%
! TATTATGGGCTGCTACAAAG+GGG + chr8.2:3301579-3301598 None:intergenic 40.0%
! TTGGTGACAGAGTTAAGTAC+TGG - chr8.2:3301437-3301456 MS.gene042247:intron 40.0%
!! ATGATCAGGTGGATTGTCTA+CGG + chr8.2:3302180-3302199 None:intergenic 40.0%
!! ATGGTGGGTGTTTACCATTT+CGG + chr8.2:3301504-3301523 None:intergenic 40.0%
!! CCGAATGTTTTTGTAGGGTA+TGG - chr8.2:3302762-3302781 MS.gene042247:intron 40.0%
!! GTGTTGGTTATGTGGCTATA+GGG - chr8.2:3302969-3302988 MS.gene042247:intron 40.0%
!! TGTGTTGGTTATGTGGCTAT+AGG - chr8.2:3302968-3302987 MS.gene042247:intron 40.0%
!! TTACTTTTAACGCTGCATCC+AGG - chr8.2:3301808-3301827 MS.gene042247:intron 40.0%
!!! CTACCGGTTTTCAATTTCGT+GGG - chr8.2:3300905-3300924 MS.gene042247:intron 40.0%
AAAGTTGTCAAGCAAGGACC+AGG + chr8.2:3303022-3303041 None:intergenic 45.0%
AACCAGTTGCTCACTGTGAT+TGG + chr8.2:3301300-3301319 None:intergenic 45.0%
ACCAGTTGCTCACTGTGATT+GGG + chr8.2:3301299-3301318 None:intergenic 45.0%
AGCAGTGCCAAAGAGAAAGT+TGG + chr8.2:3300295-3300314 None:intergenic 45.0%
AGTGCTTACAAGCTCTCCAA+TGG + chr8.2:3302470-3302489 None:intergenic 45.0%
ATCGACGCTTTGCTACTCAA+AGG - chr8.2:3301106-3301125 MS.gene042247:CDS 45.0%
ATGACACGGATACGAGAAAC+TGG - chr8.2:3303181-3303200 MS.gene042247:CDS 45.0%
ATGGCAAAGGATTGAGTGAC+TGG - chr8.2:3300436-3300455 MS.gene042247:CDS 45.0%
ATGTGAGAGGATACTTTGCC+TGG - chr8.2:3303001-3303020 MS.gene042247:CDS 45.0%
CAAAAAGATGGTGTCCCCAT+TGG - chr8.2:3302451-3302470 MS.gene042247:intron 45.0%
CAAGTACCCAAAAGAGATGG+AGG - chr8.2:3302249-3302268 MS.gene042247:intron 45.0%
CATGCTGCTACAAAGTTGTG+AGG + chr8.2:3301669-3301688 None:intergenic 45.0%
CCACTCAAAGTTGTCAAGCA+AGG + chr8.2:3303028-3303047 None:intergenic 45.0%
CCTGCAAGTATACAATTCGC+AGG - chr8.2:3301196-3301215 MS.gene042247:CDS 45.0%
CCTGCGAATTGTATACTTGC+AGG + chr8.2:3301199-3301218 None:intergenic 45.0%
CCTTGCTTGACAACTTTGAG+TGG - chr8.2:3303025-3303044 MS.gene042247:CDS 45.0%
CGCTTTGCTACTCAAAGGTT+TGG - chr8.2:3301111-3301130 MS.gene042247:CDS 45.0%
CTGTGAGCAGAAGGTAGATT+TGG - chr8.2:3301043-3301062 MS.gene042247:CDS 45.0%
CTGTTACCTATGTGAAGGAC+AGG - chr8.2:3302628-3302647 MS.gene042247:intron 45.0%
GAACTTGACGACAGATATGG+AGG - chr8.2:3301262-3301281 MS.gene042247:intron 45.0%
GCCATGGACATTGAACAATG+AGG + chr8.2:3301958-3301977 None:intergenic 45.0%
GCTTACTTGAGTGATGGCAA+AGG - chr8.2:3300423-3300442 MS.gene042247:CDS 45.0%
GTATAAACCCTCTGTTGTGG+AGG + chr8.2:3302419-3302438 None:intergenic 45.0%
GTGCCAAAGAGAAAGTTGGA+TGG + chr8.2:3300291-3300310 None:intergenic 45.0%
GTGCTTACAAGCTCTCCAAT+GGG + chr8.2:3302469-3302488 None:intergenic 45.0%
TGCTTACAAGCTCTCCAATG+GGG + chr8.2:3302468-3302487 None:intergenic 45.0%
TGGCAAAGGATTGAGTGACT+GGG - chr8.2:3300437-3300456 MS.gene042247:CDS 45.0%
TGGCTCAATGTTTACCCACA+AGG - chr8.2:3302594-3302613 MS.gene042247:intron 45.0%
TGGTTATGTGGCTATAGGGA+AGG - chr8.2:3302973-3302992 MS.gene042247:intron 45.0%
TTGACGACAGATATGGAGGT+TGG - chr8.2:3301266-3301285 MS.gene042247:intron 45.0%
TTGCAATGACTGTGAGCAGA+AGG - chr8.2:3301034-3301053 MS.gene042247:CDS 45.0%
TTTGGAGAAGTCAACTTGGC+TGG - chr8.2:3301061-3301080 MS.gene042247:CDS 45.0%
! TGAGAATGCTCCAAGAGCAT+AGG + chr8.2:3300238-3300257 None:intergenic 45.0%
! TGGAGCATTCTCATTCTCAG+AGG - chr8.2:3300245-3300264 MS.gene042247:CDS 45.0%
!! ATAATCTGGGGGCGTCAATA+AGG - chr8.2:3302865-3302884 MS.gene042247:intron 45.0%
!! CAGCTATTTTACGTGTGACC+AGG + chr8.2:3302942-3302961 None:intergenic 45.0%
!! GAAGGTGCTTACTTGAGTGA+TGG - chr8.2:3300417-3300436 MS.gene042247:CDS 45.0%
!!! GCTACCGGTTTTCAATTTCG+TGG - chr8.2:3300904-3300923 MS.gene042247:intron 45.0%
ACACCTCTTGTTCGTCTCAC+AGG + chr8.2:3301157-3301176 None:intergenic 50.0%
CAACTGCAGCTGCATGTGAT+AGG + chr8.2:3301606-3301625 None:intergenic 50.0%
CAAGGAACCTCCACAACAGA+GGG - chr8.2:3302409-3302428 MS.gene042247:intron 50.0%
CCAGTTGCTCACTGTGATTG+GGG + chr8.2:3301298-3301317 None:intergenic 50.0%
CGGCAAGTACCCAAAAGAGA+TGG - chr8.2:3302246-3302265 MS.gene042247:intron 50.0%
CTCGTATCCGTGTCATGTTC+TGG + chr8.2:3303177-3303196 None:intergenic 50.0%
CTTAGAACCGTAGGCTCAGA+GGG - chr8.2:3303228-3303247 MS.gene042247:CDS 50.0%
GCATTGTCGTACACGTTGAC+TGG - chr8.2:3301852-3301871 MS.gene042247:CDS 50.0%
GGGTACTTGCCGAAGAAGAT+TGG + chr8.2:3302238-3302257 None:intergenic 50.0%
TAGCGAGGGAGATTCAGAGA+AGG - chr8.2:3301552-3301571 MS.gene042247:CDS 50.0%
TCAATGTGTGAGTCTGGGCA+AGG - chr8.2:3302391-3302410 MS.gene042247:CDS 50.0%
TCTACTACCCTCTGAGCCTA+CGG + chr8.2:3303238-3303257 None:intergenic 50.0%
TTCGTCTCACAGGCAGTAGA+TGG + chr8.2:3301147-3301166 None:intergenic 50.0%
!! AAGATTGGGTTGGACGTGCA+AGG + chr8.2:3302539-3302558 None:intergenic 50.0%
AATTTGCGGGAGCAGCGACA+TGG + chr8.2:3301523-3301542 None:intergenic 55.0%
AGGGAAGGAGCAGATGTGAG+AGG - chr8.2:3302988-3303007 MS.gene042247:intron 55.0%
CACTGCTTCTTCTGCCTACC+AGG - chr8.2:3300308-3300327 MS.gene042247:CDS 55.0%
CCCCAATCACAGTGAGCAAC+TGG - chr8.2:3301295-3301314 MS.gene042247:intron 55.0%
CTGCCTGTGAGACGAACAAG+AGG - chr8.2:3301151-3301170 MS.gene042247:CDS 55.0%
GCAAGGAACCTCCACAACAG+AGG - chr8.2:3302408-3302427 MS.gene042247:intron 55.0%
GCTGAGCAGGGAGGACAAAT+TGG - chr8.2:3301830-3301849 MS.gene042247:CDS 55.0%
GCTTAGAACCGTAGGCTCAG+AGG - chr8.2:3303227-3303246 MS.gene042247:CDS 55.0%
GGTAGAAGTCGAACGAGCCA+TGG + chr8.2:3301974-3301993 None:intergenic 55.0%
TGTCGCTGCTCCCGCAAATT+TGG - chr8.2:3301523-3301542 MS.gene042247:intron 55.0%
TTTGTCCTCCCTGCTCAGCC+TGG + chr8.2:3301829-3301848 None:intergenic 60.0%
ACGCTGCATCCAGGCTGAGC+AGG - chr8.2:3301817-3301836 MS.gene042247:intron 65.0%
CGCTGCATCCAGGCTGAGCA+GGG - chr8.2:3301818-3301837 MS.gene042247:intron 65.0%
TGCATCCAGGCTGAGCAGGG+AGG - chr8.2:3301821-3301840 MS.gene042247:intron 65.0%
TGCGGGAGCAGCGACATGGT+GGG + chr8.2:3301519-3301538 None:intergenic 65.0%
TTGCGGGAGCAGCGACATGG+TGG + chr8.2:3301520-3301539 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 3300201 3303275 3300201 ID=MS.gene042247
chr8.2 mRNA 3300201 3303275 3300201 ID=MS.gene042247.t1;Parent=MS.gene042247
chr8.2 exon 3303147 3303275 3303147 ID=MS.gene042247.t1.exon1;Parent=MS.gene042247.t1
chr8.2 CDS 3303147 3303275 3303147 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3302996 3303062 3302996 ID=MS.gene042247.t1.exon2;Parent=MS.gene042247.t1
chr8.2 CDS 3302996 3303062 3302996 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3302823 3302881 3302823 ID=MS.gene042247.t1.exon3;Parent=MS.gene042247.t1
chr8.2 CDS 3302823 3302881 3302823 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3302534 3302609 3302534 ID=MS.gene042247.t1.exon4;Parent=MS.gene042247.t1
chr8.2 CDS 3302534 3302609 3302534 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3302349 3302423 3302349 ID=MS.gene042247.t1.exon5;Parent=MS.gene042247.t1
chr8.2 CDS 3302349 3302423 3302349 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3302171 3302258 3302171 ID=MS.gene042247.t1.exon6;Parent=MS.gene042247.t1
chr8.2 CDS 3302171 3302258 3302171 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3301828 3302080 3301828 ID=MS.gene042247.t1.exon7;Parent=MS.gene042247.t1
chr8.2 CDS 3301828 3302080 3301828 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3301531 3301646 3301531 ID=MS.gene042247.t1.exon8;Parent=MS.gene042247.t1
chr8.2 CDS 3301531 3301646 3301531 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3300996 3301251 3300996 ID=MS.gene042247.t1.exon9;Parent=MS.gene042247.t1
chr8.2 CDS 3300996 3301251 3300996 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3300792 3300894 3300792 ID=MS.gene042247.t1.exon10;Parent=MS.gene042247.t1
chr8.2 CDS 3300792 3300894 3300792 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3300590 3300698 3300590 ID=MS.gene042247.t1.exon11;Parent=MS.gene042247.t1
chr8.2 CDS 3300590 3300698 3300590 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
chr8.2 exon 3300201 3300486 3300201 ID=MS.gene042247.t1.exon12;Parent=MS.gene042247.t1
chr8.2 CDS 3300201 3300486 3300201 ID=cds.MS.gene042247.t1;Parent=MS.gene042247.t1
Gene Sequence

>MS.gene042247

ATGGAGATACAACTACTCTTTTTCTTATTCACTACCTATGCTCTTGGAGCATTCTCATTCTCAGAGGAGTTTGATCATTATCCTTTTCCATCCAACTTTCTCTTTGGCACTGCTTCTTCTGCCTACCAGGTTTCTTTACCTTCTTCATGTTTATCTCCTAAAATCTTCATTATTTTAATTAATCATGGCTGATTATTAAACTTAATATTATAGTATGAAGGTGCTTACTTGAGTGATGGCAAAGGATTGAGTGACTGGGATGTCTTCACTCATAAACCAGGTCATGATAACAACATTTCTATGTCATCTAGACATCCATTTTTCATACTTGAATACCTTCATTCATTCAATCACCTGAGTTGCGTAATTACATAATAACATATATTTTTTTCAGGTAGTACTGATGATGGAAGTAACGGTGATGTTACTGTTGATCAATATCATCGGTATCTGGTACACATCATAATACTATTTTTTGGTGAATCATCACAATACTTTTAAGTTCCTCATAATCAAAATCCAATTTAGTTTAAGAGGTTACAACCTTAACAAGTGTTGTGTCAATACTAGAATTTTAGACTTGTCGAGAAAGTATGTGTACCTTATCTACTTTAACTAATTACACCCATTATATATGGTCTAAATGTCAATTTGTAATAAAAACAGGAGGATGTAGATTTAATGGAAGCTATCAAAGTCAACAGCTACCGGTTTTCAATTTCGTGGGCAAGAATTCTACCAAGTACGCATCTTGATTTAACATAATCTCAGTGATTTCAACTAATTAGCAAATATTAAGTACCTGTGTGTTCTAGTCAATTCACAAGCAGAATTTGCAATGACTGTGAGCAGAAGGTAGATTTGGAGAAGTCAACTTGGCTGGTATTGACTACTATAATAGGCTTATCGACGCTTTGCTACTCAAAGGTTTGGTTTCAATCTTCCATCTACTGCCTGTGAGACGAACAAGAGGTGTCACTGTTCGATTAGCTTAACCTGCAAGTATACAATTCGCAGGGATCCAACCGTTTGTTACATTGTTTCACTTTGATTTCCCTCAAGAACTTGACGACAGATATGGAGGTTGGCTAAGTCCCCAATCACAGTGAGCAACTGGTTCTACAATTCAATTTGCTCATACTTCACTTCCTTTGTCATACTTTACCAGCATTATAATGTATTTTGACATGTACAGGGAAGATTTTGTATTATTCGCAGACATATGTTTTAAATCCTTTGGTGACAGAGTTAAGTACTGGACCACATTCAATGAACCAAATCTCCAAGTTTCACTTGGTTACCGAAATGGTAAACACCCACCATGTCGCTGCTCCCGCAAATTTGGAAATTGTAGCGAGGGAGATTCAGAGAAGGACCCCTTTGTAGCAGCCCATAATATTATCCTATCACATGCAGCTGCAGTTGATATTTATAGAAACAAATACCAGGTAATATAGTAAAAAGACCTCACAACTTTGTAGCAGCATGCTAAGGAATCAAATTATTTCTATTTAAGCTAATAAAAAAATAATTATGATGCTAGTTTTACAAATCAAAGTGAAAAGTAAGTATGATTGAACTCTCATTCGAAACATTGTAAACATGATTTACTTTTAACGCTGCATCCAGGCTGAGCAGGGAGGACAAATTGGCATTGTCGTACACGTTGACTGGTTTGAACCGTTTAGCAATTCGGTAGCTGATAAATTAGCTGCTGAAAGAGCTCAATCATTCAGCATGAACTGGTAGGATTTCCTCATTGTTCAATGTCCATGGCTCGTTCGACTTCTACCATGCAAAAGTTTGATTTGGATTTGTTCTACTCACATAGTCACATTTGCAACTGCCAGATCAAGATTGAACAGCTCACTTTTGACTTCATATTTTGGCCTGATTTACTAAAGTCAGAGTATGAGACTGAAATCTTGATCAAATGGTTCAAATCATGTGATTGGACTCAATGCATGATTCTCTAACCGTAGACAATCCACCTGATCATGAAGTTTGACTCTATAATAATGCAGGATCTTGGATCCAATCTTCTTCGGCAAGTACCCAAAAGAGATGGAGGTGATTTTAGGAAGTACATTACCCAAATTTTCCAGTAACGACAAAGCAAAACTGAATCGAGGACTCGATTTCATTGGCATCAATCATTATGCAGGTTACTATGCTAGAGACTGCATATCATCAATGTGTGAGTCTGGGCAAGGAACCTCCACAACAGAGGGTTTATACCAACAAACTGCACAAAAAGATGGTGTCCCCATTGGAGAGCTTGTAAGCACTTCATCCTTTTGATGATTCCATTAGTTACATTTTTATTTATCGTTAACCTTGCACGTCCAACCCAATCTTGAAAGTTGTATTGTTATGTGTAGACTCCATTTGATTGGCTCAATGTTTACCCACAAGGCATGAAGAAAACTGTTACCTATGTGAAGGACAGGTACAATAACACTCCAATGTTCATTACTGAGAATGGTAAGTAAAGAAACACATTGAAGTTGAACCATTTTCATTGAATTTCAACCATTATTAGTCTCTTACACAGACTCTATCCGAATGTTTTTGTAGGGTATGGCAATTTGTATGATCCAAATAACACCAAGGAAGAATGTCTTAATGATATTAAAAGAATAAACTACATGACTGGTCACTTAGATAATCTGGGGGCGTCAATAAGGTAAGAGTCCACAAAAACCGAATAAACATCAAAGTTATCGATATATGGTTTACCTGGTCACACGTAAAATAGCTGAGTATTTGTGTTGGTTATGTGGCTATAGGGAAGGAGCAGATGTGAGAGGATACTTTGCCTGGTCCTTGCTTGACAACTTTGAGTGGCTATTTGGTTTTACAGTGAAATTTGGACTTTACCATGTTGATTTTGCTACGCAGAAGAGAACTCCAAAATTGTCAGCTAGTTGGTACAAACATTTCATTGCAAAACATAAAACCGAAAGCAGTAATCCAGAACATGACACGGATACGAGAAACTGGAACAAACAATTCAAGGCAAATATGCTTAGAACCGTAGGCTCAGAGGGTAGTAGAATAAAAAACTTGGATTAA
Protein sequence

>MS.gene042247.t1

MEIQLLFFLFTTYALGAFSFSEEFDHYPFPSNFLFGTASSAYQYEGAYLSDGKGLSDWDVFTHKPGSTDDGSNGDVTVDQYHRYLEDVDLMEAIKVNSYRFSISWARILPKGRFGEVNLAGIDYYNRLIDALLLKGIQPFVTLFHFDFPQELDDRYGGWLSPQSQEDFVLFADICFKSFGDRVKYWTTFNEPNLQVSLGYRNGKHPPCRCSRKFGNCSEGDSEKDPFVAAHNIILSHAAAVDIYRNKYQAEQGGQIGIVVHVDWFEPFSNSVADKLAAERAQSFSMNWILDPIFFGKYPKEMEVILGSTLPKFSSNDKAKLNRGLDFIGINHYAGYYARDCISSMCESGQGTSTTEGLYQQTAQKDGVPIGELTPFDWLNVYPQGMKKTVTYVKDRYNNTPMFITENGYGNLYDPNNTKEECLNDIKRINYMTGHLDNLGASIREGADVRGYFAWSLLDNFEWLFGFTVKFGLYHVDFATQKRTPKLSASWYKHFIAKHKTESSNPEHDTDTRNWNKQFKANMLRTVGSEGSRIKNLD