AlfalfaGEDB | MS.gene006777

M. sativa cultivar XinJiangDaYe / MS.gene006777

KEGG: K01188
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00232.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006777.t1	XP_013456735.1	97.4	470	12	0	1	470	1	470	1.30E-275	958.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006777.t1	Q9FIU7	72.1	462	128	1	10	470	16	477	4.0e-209	728.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006777.t1	A0A072UN16	97.4	470	12	0	1	470	1	470	9.3e-276	958.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene002910	MS.gene006777	PPI
MS.gene003900	MS.gene006777	PPI
MS.gene006777	MS.gene57809	PPI
MS.gene006777	MS.gene75053	PPI
MS.gene006777	MS.gene37750	PPI
MS.gene006777	MS.gene05244	PPI
MS.gene006777	MS.gene20732	PPI
MS.gene006777	MS.gene54587	PPI
MS.gene006777	MS.gene05240	PPI
MS.gene006777	MS.gene54576	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006777.t1	MTR_4g081530	97.447	470	12	0	1	470	1	470	0.0	960
MS.gene006777.t1	MTR_4g083000	59.315	467	188	2	3	469	8	472	0.0	601
MS.gene006777.t1	MTR_5g044000	57.819	486	184	1	5	469	6	491	0.0	589
MS.gene006777.t1	MTR_4g083000	60.686	379	148	1	91	469	1	378	2.90e-178	506
MS.gene006777.t1	MTR_6g078450	54.324	451	200	5	23	470	35	482	2.01e-172	495
MS.gene006777.t1	MTR_6g078380	55.111	450	197	4	23	469	35	482	5.51e-172	494
MS.gene006777.t1	MTR_2g098540	48.936	470	234	4	14	480	21	487	5.93e-163	471
MS.gene006777.t1	MTR_2g098520	48.712	466	233	4	18	480	28	490	1.99e-162	469
MS.gene006777.t1	MTR_6g078330	48.954	478	229	6	5	470	9	483	1.08e-161	468
MS.gene006777.t1	MTR_4g066580	50.554	451	217	4	23	470	38	485	4.93e-161	466

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene006777.t1	AT5G54570	73.068	453	121	1	19	470	25	477	0.0	722
MS.gene006777.t1	AT5G54570	72.600	427	116	1	19	444	25	451	0.0	672
MS.gene006777.t1	AT5G54570	73.421	380	101	0	91	470	1	380	0.0	611
MS.gene006777.t1	AT1G26560	60.538	446	176	0	24	469	32	477	0.0	599
MS.gene006777.t1	AT3G18080	50.780	449	210	6	24	469	39	479	5.24e-165	476
MS.gene006777.t1	AT3G18070	48.775	449	211	7	24	469	30	462	5.50e-159	460
MS.gene006777.t1	AT3G18070	48.998	449	216	7	24	469	30	468	1.40e-157	457
MS.gene006777.t1	AT5G36890	48.430	446	215	5	26	469	18	450	6.26e-149	434
MS.gene006777.t1	AT5G36890	48.430	446	215	5	26	469	18	450	7.06e-149	434
MS.gene006777.t1	AT5G42260	47.689	476	233	7	3	470	6	473	1.25e-148	434

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Find 105 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTTTGAAGCATGTAT+AGG	0.438338	4.1:+25439887	None:intergenic
AAACTTGCTAGTGGGATCAT+TGG	0.413852	4.1:-25438941	MS.gene006777:CDS
AAAGTTGTGAGGTTCATTGA+AGG	0.577654	4.1:+25439839	None:intergenic
AAATAAAGGAGATAGCATAT+GGG	0.617078	4.1:-25441240	MS.gene006777:CDS
AACAACAAGGAGGTAAAATA+GGG	0.459784	4.1:-25439254	MS.gene006777:CDS
AACAGGGGAACCAAATAAAG+AGG	0.515074	4.1:-25440412	MS.gene006777:CDS
AACTGCTTCTTCGGCTCACC+AGG	0.478451	4.1:-25441375	MS.gene006777:intron
AACTTCTATAGGCAGCAGCA+TGG	0.595916	4.1:+25439691	None:intergenic
AAGCATGTGAAAAGTAAATA+TGG	0.303866	4.1:-25438544	MS.gene006777:CDS
AAGTTACCAACTACATTTCA+AGG	0.422523	4.1:-25439673	MS.gene006777:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACTTCCATTCAGCTTA+AGG	+	chr4.1:25440562-25440581	None:intergenic	30.0%
AACAACAAGGAGGTAAAATA+GGG	-	chr4.1:25440052-25440071	MS.gene006777:intron	30.0%
AAGTTACCAACTACATTTCA+AGG	-	chr4.1:25439633-25439652	MS.gene006777:intron	30.0%
ACAAGAGGATAAGATATCAT+AGG	-	chr4.1:25441132-25441151	MS.gene006777:intron	30.0%
ACGAAATTACTAACCATTCT+CGG	+	chr4.1:25440808-25440827	None:intergenic	30.0%
AGATAATATGAACTCAATGC+AGG	-	chr4.1:25441279-25441298	MS.gene006777:intron	30.0%
AGATATAAAGAATGCAATGC+CGG	+	chr4.1:25439706-25439725	None:intergenic	30.0%
AGGTGAATATATGATGTGTT+TGG	+	chr4.1:25439661-25439680	None:intergenic	30.0%
ATGCTGGAAGATAAATATGA+AGG	-	chr4.1:25439099-25439118	MS.gene006777:intron	30.0%
ATGTTATGAGCAACAATGTA+AGG	+	chr4.1:25439586-25439605	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	25437824	25441504	25437824	ID=MS.gene006777
chr4.1	mRNA	25437824	25441504	25437824	ID=MS.gene006777.t1;Parent=MS.gene006777
chr4.1	exon	25441376	25441504	25441376	ID=MS.gene006777.t1.exon1;Parent=MS.gene006777.t1
chr4.1	CDS	25441376	25441504	25441376	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25441219	25441285	25441219	ID=MS.gene006777.t1.exon2;Parent=MS.gene006777.t1
chr4.1	CDS	25441219	25441285	25441219	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25441066	25441124	25441066	ID=MS.gene006777.t1.exon3;Parent=MS.gene006777.t1
chr4.1	CDS	25441066	25441124	25441066	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25440522	25440597	25440522	ID=MS.gene006777.t1.exon4;Parent=MS.gene006777.t1
chr4.1	CDS	25440522	25440597	25440522	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25440367	25440438	25440367	ID=MS.gene006777.t1.exon5;Parent=MS.gene006777.t1
chr4.1	CDS	25440367	25440438	25440367	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25440186	25440273	25440186	ID=MS.gene006777.t1.exon6;Parent=MS.gene006777.t1
chr4.1	CDS	25440186	25440273	25440186	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25439674	25439929	25439674	ID=MS.gene006777.t1.exon7;Parent=MS.gene006777.t1
chr4.1	CDS	25439674	25439929	25439674	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25439162	25439277	25439162	ID=MS.gene006777.t1.exon8;Parent=MS.gene006777.t1
chr4.1	CDS	25439162	25439277	25439162	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25438830	25439053	25438830	ID=MS.gene006777.t1.exon9;Parent=MS.gene006777.t1
chr4.1	CDS	25438830	25439053	25438830	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25438706	25438737	25438706	ID=MS.gene006777.t1.exon10;Parent=MS.gene006777.t1
chr4.1	CDS	25438706	25438737	25438706	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25438515	25438617	25438515	ID=MS.gene006777.t1.exon11;Parent=MS.gene006777.t1
chr4.1	CDS	25438515	25438617	25438515	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25438142	25438250	25438142	ID=MS.gene006777.t1.exon12;Parent=MS.gene006777.t1
chr4.1	CDS	25438142	25438250	25438142	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25437952	25438027	25437952	ID=MS.gene006777.t1.exon13;Parent=MS.gene006777.t1
chr4.1	CDS	25437952	25438027	25437952	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1
chr4.1	exon	25437824	25437862	25437824	ID=MS.gene006777.t1.exon14;Parent=MS.gene006777.t1
chr4.1	CDS	25437824	25437862	25437824	ID=cds.MS.gene006777.t1;Parent=MS.gene006777.t1

Gene Sequence

>MS.gene006777

ATGAATATTAGTGTTACTAATACTATTATTCTTTTCTTTATAACCCAACTCCTTGTAAGAGCAGAATCAATTACCAGAGGAGATTTTCCAGAGGGCTTTATCTTTGGAACTGCTTCTTCGGCTCACCAGGTACGTACTATTTATTCAATCAACTTATACTTGAAATTTGAAATAGTATAAGTTTATTAAAAAATTTAAGTAATGTCTTATGTAACATAGTTTGAAGGAGCTGCTGATGAAGGAAATAAAGGAGATAGCATATGGGATACTTTCTCAAGAATACCAGGTAGAAATGAAAATACCAAAAATACTTTAGTCAACAAAATAAGATTTTAAGTTGGCAGAATTTTTATTTATTTAGTTTTCAATTATTATGATAGGAAGAATTTTGGACTTCAGTAATGCAAATATGGCAGTGGATCAGTATCACCGATTTCAGGTGACTTCAACGTTACTCTACCTAGATAGAGATAACTTGCACCAATTTTCTTTATCTTCTTCAATTACTTTGCATGTATGATGTAGCCAATATCTGTATATAGAATAAACAAGTCAGAATGAGCACCGCCTAAGTAATTAATAATGATATATGAAAGCATATAATGATGATAACGTTACACCAACTTTGTATAATAGAAAATCCGCATAAAGGGAACACTTGGAATAAAGTCCAAACGTGTAAACTGTTGCAAAACATATCTTTTGACCATATAGTTAGTTAGTAGTACGGTAGTTTTTGTATATAGAATAGAATTCACCATAACTTTGGTACAAAAAAAATTTGTTATTTGATATTAATTATTTTTTTAAAAAAATATGGTACTTCTTTTTATCTTTAGTCCTTTCAATTCATATATTACTAAAAAAATGATAATAATCATTGAAGCATATACTAATTCCATTACAGAATGACATAAACTTGATGAAAGATTTGGGAATGGATTCTTATAGATTTTCCATATCATGGGCAAGAATTTTCCCAAGTAAGTTACAATATTTTCAAAACCTTATGTAATTTGTTTGTACCTTGGTAGATGTTAATGCTAATAACAGCATTTTGTAACAGACGGAACAGGGGAACCAAATAAAGAGGGAATAAAATATTACAACTCCCTCATTGATGCTTTATTAGAAAAAGGTTACTAGAGAATTCATTTTGAAATGTGATCTTAAAAATAGAAAAAAAAAATACTAAGTAACACTAAATTTGTATTATTTTCTATCCACTTAGGAATCCAGCCTTTTGTAACTCTATATCATTGGGATCTTCCACAGATGCTGGAAGATAAATATGAAGGATGGTTAAGTACACAAATCATGTAAGTTCTCCAATGTTATATAGCATGCATAGTATCAACATTTCGAGTATCTAGGTATCCGACTCCGACACATTGACACGACACCGATACATGTGATTATGTTCAAATTAATTCATTTTCTAAAGTTACTATCGATGTTGACGTGTCAGTGACGTCGTGTCTGGTGTCCATGTTTGTGTGTCAGCGCTTTATAGCATAAAGGTCTTTTGAGAAAGATAGTTTTCTGAACTTCATATTTATCTCTAATTATTCACAGAAAAGATTTTGAGCATTATGCCTATACATGCTTCAAAGCTTTTGGAGACAGAGTTAAGCACTGGATTACCTTCAATGAACCTCACAACTTTGCCCTCCATGGCTATGATTTAGGCATTCAAGCACCAGGAAGATGTTCCCTTCTTGGTCATCTTGTATGTAAGAAGGGAAAATCGTCCACTGAACCTTACATTGTTGCTCATAACATTCTCTTATCCCATGCTGCTGCCTATAGAAGTTACCAACTACATTTCAAGGTTCCAAACACATCATATATTCACCTTATAATAATAAAAAAAATATTACCGGCATTGCATTCTTTATATCTCAAACAACGTTTCAAAAATCAAACTGGCTGGCTCAACCGGGAGATTTCATCCCGATTACATTGTAGTCCGATGAGCCAAATTGAACCGGTTCAACGCGGTTGGTTCAATCAGTTTTCATTTTTATGTTTTTTATTTATGTATTTGCCATCTGACTCTTCCAGGAGATGAAGGTCTTACCGGTTCAATCTCTAGTCTGATTTTTAGGACATTGATCTCACATGTTGAAATGAGTGTGTCGTTAGCATTTCTTAGTGAACAAAACAAGAAGTCTGTAAAGAAGCTGAAAATATCAATAATATTTGTGTTATTCGGCGGATAATCCAGGAACAACAAGGAGGTAAAATAGGGATAGCACTAGATGCTGTTTGGTACGAACCTATAACTGAACTTGATGAAGACGAAGAAGCAGCTGCTAGAGCTATGGACTTTTCACTGGGATGGTAACCTTACTCATAGCTTAATGATTAATTGACTTTCACAATATTTGATAGCTTAATTTGTGACAACTAAGAAATTAAGAACTCATCTTCCACACTAATGTGAATCAGGTTCCTTGACCCTCTTATCTTTGGAAAATATCCTATCTCAATGCAAAAACTTGTATCTGAAAGATTGCCAGAGATTACTGATGCAACCTCAAACTTGCTAGTGGGATCATTGGATTTTATTGGCATAAACCATTACACTTCATTGTATGCTCGAAACGACAGGACTCGGATTCATAAACTGATTATGCAAGATGCTATATCTGATGCTGCTGTTATTACAACAGGTAAGCTGTTATTGATGCAAATTTTGAAACCTTTTTTTTGTTAATTTAATCAATTCATAACCTTAAGCTGAATGGAAGTTTTTTATATTTAGCATACAGAAGAGGAGCTGCAATTGGAGATAAGGTATGCTCAGAAATTAAACCACCATTGCTGAAATTTACATATACACTTCATGCTATTAAATCAACACCAATTTCATTTTTTTATATAGGCAGCTTCAAGTTGGCTACACATTGTCCCATGGGGAATCAGAAAATTAATGAAGCATGTGAAAAGTAAATATGGAAACACACCAGTGATCATAACCGAGAATGGTTAGTAATTTCGTCTGCAGAAGTCAGTCTTGATTGAGTTTAATAATTTCTATGTATTACCGACACCTCAGATTGAAGGCACGTGTCGGACACCGACACATGTGATAACATTTCATTATTCAATTTTCTCAAATTATTACACGTGTCAACATGTGTCAGCGTCAATGTCGTGGTTTGTGTCAGTGCGCTATAAATTCATAGATATTAGATCCATCTCAGTGCTCAGATTATTTTTTTTAACATTTATTATATTTTTTACTGCAGGAATGGATGAAGCAAGTAGACCCTTGATGACCTTACAAAAGGCATTAAATGATGACAAGAGGATAAGATATCATAGGGACTATCTCTCAAATCTATCAGCTGCTATAAGGTATTAATTATTATAGCAATTCATTAGTCCTTGAATTTTTGCATCTAGATTATGTAACTATATAAGATTAATTGGTAACTCCCTTGATCCTGAGATAATATGAACTCAATGCAGGGAAGATGGTTGCAATGTGAGGGGTTACTTTGTGTGGTCATTGCTTGATAATTGGGAGTGGAACATGGGATATACAGTACGCTTTGGGCTCTACTATGTGGATTTCAAAAACAATCTTACTAGGATACCAAAATCTTCTGTACAATGGTTCAAAAATATGCTCAGATTGGAATCTGGAAAAATATGCTCATACATAGAAAATAG

Protein sequence

>MS.gene006777.t1

MNISVTNTIILFFITQLLVRAESITRGDFPEGFIFGTASSAHQFEGAADEGNKGDSIWDTFSRIPGRILDFSNANMAVDQYHRFQNDINLMKDLGMDSYRFSISWARIFPNGTGEPNKEGIKYYNSLIDALLEKGIQPFVTLYHWDLPQMLEDKYEGWLSTQIIKDFEHYAYTCFKAFGDRVKHWITFNEPHNFALHGYDLGIQAPGRCSLLGHLVCKKGKSSTEPYIVAHNILLSHAAAYRSYQLHFKEQQGGKIGIALDAVWYEPITELDEDEEAAARAMDFSLGWFLDPLIFGKYPISMQKLVSERLPEITDATSNLLVGSLDFIGINHYTSLYARNDRTRIHKLIMQDAISDAAVITTAYRRGAAIGDKAASSWLHIVPWGIRKLMKHVKSKYGNTPVIITENGMDEASRPLMTLQKALNDDKRIRYHRDYLSNLSAAIREDGCNVRGYFVWSLLDNWEWNMGYTIGIWKNMLIHRK