AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003905

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003905.t1 MTR_4g066580 95.030 503 15 1 1 493 1 503 0.0 987
MS.gene003905.t1 MTR_3g028720 86.879 503 55 2 1 493 1 502 0.0 922
MS.gene003905.t1 MTR_3g028720 86.739 460 50 2 1 450 1 459 0.0 838
MS.gene003905.t1 MTR_4g066580 93.925 428 16 1 1 418 1 428 0.0 828
MS.gene003905.t1 MTR_4g066590 73.253 501 117 3 3 493 4 497 0.0 780
MS.gene003905.t1 MTR_3g028720 86.854 426 45 2 1 416 1 425 0.0 776
MS.gene003905.t1 MTR_4g066630 74.741 483 111 2 21 493 22 503 0.0 773
MS.gene003905.t1 MTR_2g098540 68.191 503 142 2 1 493 1 495 0.0 724
MS.gene003905.t1 MTR_2g098520 68.560 493 145 1 11 493 6 498 0.0 720
MS.gene003905.t1 MTR_2g098790 66.798 506 151 4 1 493 1 502 0.0 717
MS.gene003905.t1 MTR_3g028720 86.154 390 43 2 1 380 1 389 0.0 711
MS.gene003905.t1 MTR_4g066630 74.715 439 100 2 21 449 22 459 0.0 701
MS.gene003905.t1 MTR_6g078450 66.799 503 154 3 1 493 1 500 0.0 700
MS.gene003905.t1 MTR_4g066210 63.762 505 170 2 2 493 3 507 0.0 689
MS.gene003905.t1 MTR_4g066640 65.923 493 154 3 12 493 12 501 0.0 688
MS.gene003905.t1 MTR_4g066240 64.646 495 165 1 9 493 6 500 0.0 686
MS.gene003905.t1 MTR_6g078330 65.675 504 159 4 1 493 1 501 0.0 683
MS.gene003905.t1 MTR_4g066240 64.516 496 165 2 9 493 6 501 0.0 682
MS.gene003905.t1 MTR_2g098540 70.404 446 122 1 58 493 6 451 0.0 676
MS.gene003905.t1 MTR_6g078380 64.881 504 163 4 1 493 1 501 0.0 662
MS.gene003905.t1 MTR_5g071520 63.548 513 166 4 1 493 1 512 0.0 659
MS.gene003905.t1 MTR_4g066630 73.762 404 95 2 21 414 22 424 0.0 639
MS.gene003905.t1 MTR_6g078450 66.957 460 139 3 1 450 1 457 0.0 635
MS.gene003905.t1 MTR_4g067040 72.263 411 96 3 3 402 4 407 0.0 625
MS.gene003905.t1 MTR_4g066330 60.433 508 159 5 1 493 1 481 0.0 619
MS.gene003905.t1 MTR_4g066340 67.536 422 127 1 82 493 6 427 0.0 608
MS.gene003905.t1 MTR_3g026280 60.814 467 168 3 37 493 20 481 0.0 599
MS.gene003905.t1 MTR_4g067040 71.684 392 93 3 3 383 4 388 0.0 592
MS.gene003905.t1 MTR_4g066280 59.831 473 152 3 33 493 35 481 0.0 590
MS.gene003905.t1 MTR_6g078330 68.766 397 114 1 107 493 1 397 0.0 578
MS.gene003905.t1 MTR_4g066240 64.183 416 139 1 9 414 6 421 0.0 572
MS.gene003905.t1 MTR_4g023000 58.030 467 180 4 37 493 22 482 0.0 568
MS.gene003905.t1 MTR_4g066630 73.315 356 84 2 21 366 22 376 0.0 560
MS.gene003905.t1 MTR_6g078330 66.753 385 116 2 119 493 15 397 0.0 540
MS.gene003905.t1 MTR_4g015420 56.341 481 160 8 35 493 61 513 0.0 538
MS.gene003905.t1 MTR_3g026620 53.000 500 218 5 4 493 6 498 0.0 534
MS.gene003905.t1 MTR_3g026400 53.307 499 213 7 6 493 8 497 0.0 526
MS.gene003905.t1 MTR_4g015460 54.800 500 175 10 16 493 19 489 0.0 523
MS.gene003905.t1 MTR_4g066640 63.265 392 130 3 1 381 1 389 0.0 516
MS.gene003905.t1 MTR_2g043200 56.223 466 160 7 44 493 70 507 3.40e-180 515
MS.gene003905.t1 MTR_6g078330 65.147 373 117 2 3 365 4 373 4.64e-179 507
MS.gene003905.t1 MTR_3g026400 54.566 449 188 5 56 493 23 466 1.66e-172 494
MS.gene003905.t1 MTR_4g083000 50.417 480 218 7 28 493 18 491 1.61e-171 493
MS.gene003905.t1 MTR_5g044000 48.031 508 223 7 24 493 6 510 2.68e-166 480
MS.gene003905.t1 MTR_4g081530 49.893 469 219 5 38 493 23 488 1.56e-160 465
MS.gene003905.t1 MTR_0110s0040 47.094 499 243 7 5 493 13 500 3.71e-157 456
MS.gene003905.t1 MTR_0110s0030 45.691 499 250 7 5 493 13 500 1.97e-153 447
MS.gene003905.t1 MTR_3g033810 46.695 469 225 6 36 493 18 472 9.54e-148 432
MS.gene003905.t1 MTR_3g033790 46.809 470 216 8 39 493 19 469 1.03e-147 432
MS.gene003905.t1 MTR_2g079550 45.582 498 243 8 10 493 8 491 1.05e-145 428
MS.gene003905.t1 MTR_2g079580 44.181 507 255 8 1 493 1 493 3.81e-145 426
MS.gene003905.t1 MTR_4g131810 46.638 461 229 6 44 490 33 490 7.73e-144 423
MS.gene003905.t1 MTR_2g022760 47.588 456 222 6 48 490 45 496 1.23e-141 417
MS.gene003905.t1 MTR_4g083000 50.374 401 181 5 107 493 1 397 2.34e-138 405
MS.gene003905.t1 MTR_7g068910 45.203 469 229 8 38 493 30 483 4.80e-137 405
MS.gene003905.t1 MTR_2g022710 43.320 494 252 7 5 484 10 489 3.49e-136 403
MS.gene003905.t1 MTR_3g026400 56.125 351 137 5 6 346 8 351 6.56e-135 395
MS.gene003905.t1 MTR_4g131800 46.799 453 226 5 44 484 521 970 6.20e-134 412
MS.gene003905.t1 MTR_7g068910 44.259 479 229 9 38 493 30 493 1.08e-133 397
MS.gene003905.t1 MTR_5g068740 44.167 480 227 11 33 493 21 478 1.16e-132 394
MS.gene003905.t1 MTR_4g016920 44.111 467 234 7 38 492 26 477 1.10e-130 389
MS.gene003905.t1 MTR_7g033860 44.043 470 228 8 41 493 50 501 5.43e-128 382
MS.gene003905.t1 MTR_1g088610 44.770 478 226 12 33 493 21 477 1.27e-127 385
MS.gene003905.t1 MTR_0108s0030 42.765 463 226 4 41 493 5 438 2.50e-124 370
MS.gene003905.t1 MTR_2g079580 44.159 428 212 7 1 414 1 415 2.23e-121 362
MS.gene003905.t1 MTR_0110s0040 48.370 368 172 5 5 362 13 372 2.05e-117 351
MS.gene003905.t1 MTR_2g022710 41.739 460 240 7 5 450 10 455 3.41e-116 350
MS.gene003905.t1 MTR_2g022710 41.739 460 240 7 5 450 10 455 5.37e-116 350
MS.gene003905.t1 MTR_7g051610 43.939 462 229 7 40 490 76 518 6.79e-115 349
MS.gene003905.t1 MTR_2g022760 46.599 397 195 6 107 490 1 393 2.54e-114 343
MS.gene003905.t1 MTR_7g051510 43.011 465 229 9 40 490 76 518 8.74e-114 346
MS.gene003905.t1 MTR_7g051650 42.796 465 231 8 40 490 76 519 3.78e-112 342
MS.gene003905.t1 MTR_2g022710 42.689 424 215 7 5 414 10 419 5.14e-112 338
MS.gene003905.t1 MTR_7g051650 42.366 465 229 8 40 490 76 515 1.21e-110 338
MS.gene003905.t1 MTR_2g022710 44.103 390 204 4 107 484 1 388 5.37e-109 330
MS.gene003905.t1 MTR_1g088630 43.077 455 193 14 58 493 14 421 6.45e-108 328
MS.gene003905.t1 MTR_7g068900 43.171 410 197 8 102 493 17 408 1.61e-106 326
MS.gene003905.t1 MTR_2g022710 41.990 412 211 7 5 402 10 407 9.88e-106 322
MS.gene003905.t1 MTR_4g016920 42.462 398 203 6 107 492 1 384 1.32e-102 314
MS.gene003905.t1 MTR_1g088630 49.379 322 117 7 41 348 29 318 5.87e-100 305
MS.gene003905.t1 MTR_2g022710 42.636 387 194 7 5 377 10 382 8.68e-98 301
MS.gene003905.t1 MTR_7g056747 41.397 401 199 5 101 490 9 384 7.57e-93 288
MS.gene003905.t1 MTR_1g088630 52.788 269 105 5 41 295 29 289 5.83e-91 280
MS.gene003905.t1 MTR_4g016920 48.889 270 125 3 107 365 1 268 9.13e-86 266
MS.gene003905.t1 MTR_0110s0020 48.638 257 124 4 5 261 13 261 1.16e-82 259
MS.gene003905.t1 MTR_4g122980 27.456 397 219 12 103 490 183 519 9.01e-30 123
MS.gene003905.t1 MTR_5g069350 42.236 161 52 6 343 493 48 177 2.67e-23 97.4
MS.gene003905.t1 MTR_8g038650 40.708 113 59 2 238 350 48 152 5.82e-22 92.8
MS.gene003905.t1 MTR_8g038650 47.143 70 36 1 48 117 7 75 6.94e-13 67.0
MS.gene003905.t1 MTR_4g061170 66.667 42 12 1 451 490 23 64 7.26e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003905.t1 AT2G44480 57.388 467 188 2 37 493 33 498 0.0 566
MS.gene003905.t1 AT2G44480 57.388 467 188 2 37 493 64 529 0.0 566
MS.gene003905.t1 AT5G42260 53.614 498 208 5 7 493 6 491 0.0 545
MS.gene003905.t1 AT5G44640 52.823 496 211 5 9 493 8 491 0.0 542
MS.gene003905.t1 AT2G44450 55.484 465 192 3 39 493 31 490 0.0 540
MS.gene003905.t1 AT3G60130 54.077 466 198 4 39 493 30 490 0.0 523
MS.gene003905.t1 AT3G60140 49.699 499 225 6 8 493 7 492 5.14e-179 514
MS.gene003905.t1 AT5G24550 52.643 473 201 7 39 493 32 499 3.33e-178 511
MS.gene003905.t1 AT2G25630 50.704 497 205 6 8 493 6 473 6.90e-176 503
MS.gene003905.t1 AT1G51470 52.350 468 199 8 38 493 44 499 3.61e-172 494
MS.gene003905.t1 AT5G24540 51.173 469 214 6 39 493 32 499 8.45e-172 494
MS.gene003905.t1 AT2G44470 49.573 468 222 5 39 493 29 495 1.78e-170 493
MS.gene003905.t1 AT1G47600 51.923 468 201 8 38 493 44 499 3.65e-170 489
MS.gene003905.t1 AT1G47600 51.923 468 200 8 38 493 44 498 2.25e-169 488
MS.gene003905.t1 AT2G44460 49.469 471 225 5 35 493 25 494 4.13e-168 487
MS.gene003905.t1 AT3G60120 50.427 468 219 5 38 493 13 479 1.56e-166 481
MS.gene003905.t1 AT2G44490 50.213 470 220 5 38 493 13 482 3.68e-166 481
MS.gene003905.t1 AT1G26560 49.260 473 220 6 36 493 29 496 1.18e-165 478
MS.gene003905.t1 AT2G44480 55.668 397 165 2 107 493 1 396 7.21e-165 473
MS.gene003905.t1 AT5G54570 50.318 471 215 5 38 493 29 495 2.80e-164 476
MS.gene003905.t1 AT3G18080 49.257 471 221 7 34 493 34 497 1.63e-156 455
MS.gene003905.t1 AT2G32860 46.895 467 234 5 38 491 93 558 4.71e-153 449
MS.gene003905.t1 AT5G25980 45.968 496 250 8 3 486 15 504 5.91e-153 447
MS.gene003905.t1 AT3G09260 47.009 468 233 6 39 493 35 500 2.99e-152 444
MS.gene003905.t1 AT3G60130 52.723 404 175 4 101 493 40 438 4.55e-151 439
MS.gene003905.t1 AT2G32860 46.039 467 239 4 38 491 93 559 5.43e-151 444
MS.gene003905.t1 AT3G03640 43.444 511 262 6 1 493 1 502 2.14e-150 440
MS.gene003905.t1 AT5G24540 49.764 424 199 5 39 449 32 454 3.25e-149 434
MS.gene003905.t1 AT2G44470 47.754 423 208 4 39 449 29 450 3.36e-148 431
MS.gene003905.t1 AT3G18070 45.860 471 229 8 34 493 25 480 1.38e-147 431
MS.gene003905.t1 AT5G36890 48.172 465 218 5 40 493 17 469 1.43e-147 431
MS.gene003905.t1 AT5G36890 48.172 465 218 5 40 493 17 469 2.46e-147 431
MS.gene003905.t1 AT5G28510 46.835 474 232 7 38 493 38 509 5.54e-147 431
MS.gene003905.t1 AT3G18070 45.860 471 235 8 34 493 25 486 1.22e-146 429
MS.gene003905.t1 AT1G75940 46.203 474 233 8 39 493 37 507 2.22e-146 430
MS.gene003905.t1 AT3G21370 45.588 476 233 9 38 493 32 501 3.15e-146 429
MS.gene003905.t1 AT1G66280 46.016 502 245 9 6 493 11 500 1.16e-145 427
MS.gene003905.t1 AT3G60140 48.210 419 192 5 8 414 7 412 2.13e-145 424
MS.gene003905.t1 AT5G54570 49.415 427 198 4 38 450 29 451 6.16e-145 423
MS.gene003905.t1 AT3G60130 51.980 404 167 5 101 493 40 427 6.11e-144 421
MS.gene003905.t1 AT1G66270 45.455 484 250 7 22 493 19 500 2.61e-143 422
MS.gene003905.t1 AT1G52400 45.223 471 240 8 38 493 38 505 2.18e-142 419
MS.gene003905.t1 AT1G52400 45.223 471 240 8 38 493 38 505 2.18e-142 419
MS.gene003905.t1 AT5G26000 45.040 504 240 11 7 486 3 493 2.86e-141 417
MS.gene003905.t1 AT1G66270 45.041 484 250 8 22 493 19 498 1.20e-139 412
MS.gene003905.t1 AT1G02850 45.708 466 224 8 40 493 27 475 1.46e-136 403
MS.gene003905.t1 AT5G25980 44.880 459 236 7 3 450 15 467 2.53e-136 402
MS.gene003905.t1 AT5G25980 44.880 459 236 7 3 450 15 467 2.53e-136 402
MS.gene003905.t1 AT2G44480 62.252 302 103 2 37 328 64 364 1.57e-135 397
MS.gene003905.t1 AT5G54570 50.000 402 182 5 107 493 1 398 1.81e-134 396
MS.gene003905.t1 AT1G02850 43.763 489 223 8 40 493 27 498 3.23e-133 395
MS.gene003905.t1 AT4G27830 44.186 473 233 8 35 493 19 474 3.23e-133 395
MS.gene003905.t1 AT1G02850 44.082 490 221 9 40 493 27 499 5.21e-132 392
MS.gene003905.t1 AT1G61820 43.763 473 250 7 34 493 25 494 2.58e-131 390
MS.gene003905.t1 AT4G27820 44.468 470 228 8 38 493 22 472 1.89e-130 388
MS.gene003905.t1 AT4G21760 41.667 492 263 8 5 484 24 503 1.84e-129 387
MS.gene003905.t1 AT1G02850 45.395 456 213 9 40 493 27 448 8.16e-129 383
MS.gene003905.t1 AT1G60090 41.667 492 243 9 12 490 8 468 1.77e-128 383
MS.gene003905.t1 AT2G44450 52.326 344 149 3 160 493 1 339 8.71e-126 371
MS.gene003905.t1 AT5G26000 44.325 467 224 10 7 450 3 456 1.42e-125 374
MS.gene003905.t1 AT2G44470 49.552 335 157 3 39 362 29 362 2.96e-125 371
MS.gene003905.t1 AT3G62750 42.161 472 231 9 36 493 20 463 1.38e-124 373
MS.gene003905.t1 AT1G60090 40.200 500 246 10 12 490 8 475 3.35e-124 372
MS.gene003905.t1 AT3G62750 42.161 472 231 9 36 493 46 489 3.95e-124 372
MS.gene003905.t1 AT1G61810 42.553 470 250 7 39 493 33 497 1.05e-123 371
MS.gene003905.t1 AT4G21760 41.429 490 257 10 5 484 24 493 1.72e-123 371
MS.gene003905.t1 AT1G61810 42.612 467 248 7 39 490 33 494 1.94e-123 371
MS.gene003905.t1 AT1G52400 44.471 425 219 7 39 449 39 460 1.67e-122 366
MS.gene003905.t1 AT4G22100 41.612 459 239 7 40 486 23 464 3.64e-122 367
MS.gene003905.t1 AT1G45191 41.239 468 230 9 40 490 30 469 2.86e-117 354
MS.gene003905.t1 AT1G02850 44.988 409 180 6 40 414 27 424 8.31e-117 351
MS.gene003905.t1 AT1G45191 40.302 464 246 9 40 490 30 475 4.92e-115 349
MS.gene003905.t1 AT1G45191 40.476 462 245 8 40 486 30 476 1.92e-114 347
MS.gene003905.t1 AT1G45191 39.706 476 249 9 40 490 30 492 1.83e-112 343
MS.gene003905.t1 AT3G62740 39.293 481 253 11 28 493 12 468 5.07e-112 341
MS.gene003905.t1 AT3G62750 41.860 430 209 8 36 452 46 447 3.84e-111 337
MS.gene003905.t1 AT2G44460 48.851 348 166 4 158 493 1 348 2.01e-110 334
MS.gene003905.t1 AT5G48375 40.685 467 197 10 17 471 17 415 9.15e-109 330
MS.gene003905.t1 AT1G51490 48.997 349 151 8 46 381 27 361 5.18e-106 322
MS.gene003905.t1 AT3G18070 46.246 333 163 5 34 356 25 351 1.05e-103 315
MS.gene003905.t1 AT4G27830 42.394 401 202 7 107 493 1 386 1.58e-102 313
MS.gene003905.t1 AT3G62750 46.462 325 148 5 36 350 20 328 1.92e-102 311
MS.gene003905.t1 AT3G62750 46.462 325 148 5 36 350 20 328 1.92e-102 311
MS.gene003905.t1 AT1G60270 45.087 346 171 5 39 372 24 362 3.86e-102 311
MS.gene003905.t1 AT3G62740 39.679 436 225 10 28 449 12 423 2.32e-101 311
MS.gene003905.t1 AT3G62750 46.154 325 149 4 36 350 46 354 2.45e-101 311
MS.gene003905.t1 AT2G44480 50.347 288 132 2 216 493 6 292 1.51e-100 305
MS.gene003905.t1 AT4G27820 42.145 401 204 7 107 493 1 387 1.80e-99 306
MS.gene003905.t1 AT4G27820 42.145 401 204 7 107 493 1 387 1.80e-99 306
MS.gene003905.t1 AT1G61810 41.176 391 211 6 39 414 33 419 1.93e-99 306
MS.gene003905.t1 AT1G61810 42.537 402 211 7 107 493 1 397 2.00e-99 305
MS.gene003905.t1 AT1G45191 46.395 319 153 4 40 348 30 340 2.10e-99 305
MS.gene003905.t1 AT1G45191 46.395 319 153 4 40 348 30 340 3.01e-99 305
MS.gene003905.t1 AT3G62750 38.903 401 208 8 107 493 1 378 1.20e-90 283
MS.gene003905.t1 AT3G62750 38.903 401 208 8 107 493 1 378 1.20e-90 283
MS.gene003905.t1 AT3G18070 44.762 315 159 5 190 493 99 409 2.92e-89 280
MS.gene003905.t1 AT3G18070 47.059 68 35 1 34 101 25 91 6.58e-14 73.9
MS.gene003905.t1 AT1G61820 41.808 354 191 6 153 493 4 355 8.04e-87 271
MS.gene003905.t1 AT3G18070 44.186 301 153 5 204 493 1 297 1.71e-83 261
MS.gene003905.t1 AT1G61810 45.185 270 136 3 39 298 33 300 1.16e-78 248
MS.gene003905.t1 AT1G60270 43.885 278 141 4 107 372 1 275 6.17e-74 235
MS.gene003905.t1 AT3G18070 44.304 237 120 3 191 416 100 335 3.07e-63 209
MS.gene003905.t1 AT3G18070 47.059 68 35 1 34 101 25 91 4.39e-14 73.9
MS.gene003905.t1 AT3G18070 43.304 224 115 3 204 416 1 223 8.88e-58 191
MS.gene003905.t1 AT5G16580 35.430 302 152 8 179 460 21 299 5.78e-45 160
MS.gene003905.t1 AT3G06510 27.885 416 229 16 102 490 159 530 2.56e-28 119
MS.gene003905.t1 AT3G06510 27.020 396 224 12 102 490 159 496 8.15e-28 117

Find 101 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTATTTATTTCAGGTTTA+TGG 0.091209 4.4:+39623124 MS.gene003905:intron
GTTTGCTTTGTATTGAATTT+TGG 0.170509 4.4:-39623206 None:intergenic
GAACTTTGTTTCAAGGAATT+TGG 0.174740 4.4:+39622692 MS.gene003905:CDS
TTCCCTCAAGGCTTTATCTT+TGG 0.178608 4.4:+39621593 MS.gene003905:CDS
TCCCTCAAGGCTTTATCTTT+GGG 0.200749 4.4:+39621594 MS.gene003905:CDS
TCTATTTACATCACAGAAAA+TGG 0.202373 4.4:+39623573 MS.gene003905:CDS
GCCCATTTGCATAGCCATTT+TGG 0.208775 4.4:-39622763 None:intergenic
GAATCACCTCCGGTACAATT+TGG 0.240733 4.4:-39622821 None:intergenic
AAATCTCAAAAGGGCGTAAT+AGG 0.261458 4.4:+39622926 MS.gene003905:CDS
TAGTTACTTAACAGATTCTT+TGG 0.293237 4.4:+39623322 MS.gene003905:CDS
ACCATAATGCAGACAACTTC+TGG 0.323700 4.4:-39624039 None:intergenic
TTCTGCGATCGAGCCCTATT+TGG 0.357111 4.4:+39622841 MS.gene003905:CDS
CCATGGAGCTACGGCCAAAA+TGG 0.358635 4.4:+39622749 MS.gene003905:CDS
GTGGTAGAACACCAAGTATA+TGG 0.359362 4.4:+39621785 MS.gene003905:CDS
TTGGTAATGCGTAACCAAAT+AGG 0.369016 4.4:-39622855 None:intergenic
TCCGGTTATAGTGTCAGATT+TGG 0.370211 4.4:+39623977 MS.gene003905:CDS
TTTATCTTCGATCTGCCATT+AGG 0.377497 4.4:+39623799 MS.gene003905:CDS
CACCGGGTCGTTGCTCTAGT+TGG 0.384109 4.4:+39622792 MS.gene003905:CDS
GGCCAAAATGGCTATGCAAA+TGG 0.384332 4.4:+39622761 MS.gene003905:CDS
CAATATTCTAGACCAGGATA+TGG 0.394308 4.4:-39622167 None:intergenic
TGCAAATGGGCGAATGGCAC+CGG 0.395385 4.4:+39622775 MS.gene003905:CDS
TTGGATACTCACACTATAAG+AGG 0.395389 4.4:-39622563 None:intergenic
GAATTGCAGTTGAGCGCAAC+GGG 0.402711 4.4:+39623451 MS.gene003905:intron
GGATAATCTCCTGATGTTAG+TGG 0.412747 4.4:-39623149 None:intergenic
GATAAAATAGATGACGATAA+TGG 0.413825 4.4:-39623782 None:intergenic
TGGTAATGCGTAACCAAATA+GGG 0.414370 4.4:-39622854 None:intergenic
CTATGCGGAACTTTGTTTCA+AGG 0.415130 4.4:+39622685 MS.gene003905:CDS
GAAGCAATCTTAATACCAAT+AGG 0.417398 4.4:-39623477 None:intergenic
TACTCTTCTTCTAAAGTTTG+AGG 0.419592 4.4:-39622524 None:intergenic
TTGACATTGCTTCATTGAAT+CGG 0.421400 4.4:+39621564 MS.gene003905:CDS
TGGTGATAGAGTCAAGTATT+GGG 0.427511 4.4:+39622712 MS.gene003905:CDS
CAAGGCTTTATCTTTGGGGC+TGG 0.428623 4.4:+39621599 MS.gene003905:CDS
AAAGTACAAGAAATCTCAAA+AGG 0.432795 4.4:+39622916 MS.gene003905:intron
GCATAACCTTAGTAGCGAAT+TGG 0.439687 4.4:+39622948 MS.gene003905:CDS
CCCCAAAGATAAAGCCTTGA+GGG 0.442020 4.4:-39621595 None:intergenic
TTGGTGATAGAGTCAAGTAT+TGG 0.444972 4.4:+39622711 MS.gene003905:CDS
CAAACTTTAGAAGAAGAGTA+TGG 0.448230 4.4:+39622527 MS.gene003905:CDS
TATTGGTATTAAGATTGCTT+CGG 0.448616 4.4:+39623479 MS.gene003905:CDS
GCCAAAATGGCTATGCAAAT+GGG 0.450781 4.4:+39622762 MS.gene003905:CDS
ATCAGTTGAGGAATCACTCT+TGG 0.455004 4.4:+39623740 MS.gene003905:CDS
AATGAAGCAATGTCAAGAAT+AGG 0.456359 4.4:-39621557 None:intergenic
TCCAAATCTGACACTATAAC+CGG 0.458035 4.4:-39623978 None:intergenic
TGTTTGTATGATTAGTTTGA+AGG 0.458490 4.4:+39621748 MS.gene003905:intron
ACTCTTCTTCTAAAGTTTGA+GGG 0.459763 4.4:-39622523 None:intergenic
CCCTCAAGGCTTTATCTTTG+GGG 0.461443 4.4:+39621595 MS.gene003905:CDS
AAGCAAACTAAACTACTCAT+TGG 0.461970 4.4:+39623221 MS.gene003905:CDS
ACCTTTGTCGACTATAAGAA+TGG 0.464824 4.4:+39624004 MS.gene003905:CDS
ATGTCAAGGGTTATTATGTA+TGG 0.467423 4.4:+39623927 MS.gene003905:CDS
CAAACAGATGGAAAGCTTAG+TGG 0.481170 4.4:+39622306 MS.gene003905:intron
ATCAACGAGCTACTAGCTAA+TGG 0.482370 4.4:+39622369 MS.gene003905:CDS
AAAAGATCACGAATTCCTCT+TGG 0.485304 4.4:-39623519 None:intergenic
GGCTGGTTCATCTTCCTACC+AGG 0.487180 4.4:+39621616 MS.gene003905:CDS
GAGCGCAACGGGAAACCTAT+TGG 0.489460 4.4:+39623462 MS.gene003905:CDS
AAGATACCAATTCGCTACTA+AGG 0.501344 4.4:-39622954 None:intergenic
CAAAAGTAATAAAGAAGAGA+AGG 0.503252 4.4:-39621478 None:intergenic
CAATGGAAAAGAGTCACTAA+TGG 0.516481 4.4:-39622494 None:intergenic
CAGCGGCATGTGCAAGAAGT+TGG 0.521028 4.4:-39622874 None:intergenic
TTTATGGATCCACTAACATC+AGG 0.523193 4.4:+39623140 MS.gene003905:CDS
AAGATCAAGGATGGAAGCAA+TGG 0.523398 4.4:+39621937 MS.gene003905:CDS
ACATTCACTCATAGATATCC+TGG 0.528617 4.4:+39621811 MS.gene003905:CDS
GTATTAAGATTGCTTCGGAA+TGG 0.531040 4.4:+39623484 MS.gene003905:CDS
TGGTAGAACACCAAGTATAT+GGG 0.535693 4.4:+39621786 MS.gene003905:CDS
AAAGAATCTGTTAAGTAACT+AGG 0.540529 4.4:-39623320 None:intergenic
TATTTAATTAATTACCTGGT+AGG 0.543420 4.4:-39621630 None:intergenic
GGGTTACTTTGAATGAGCCA+TGG 0.549157 4.4:+39622732 MS.gene003905:CDS
TTGACATGCAGAAAAGATCA+AGG 0.553913 4.4:+39621924 MS.gene003905:intron
TGGTTGAATCCAAATTGTAC+CGG 0.562770 4.4:+39622812 MS.gene003905:CDS
TTGAATGAGCCATGGAGCTA+CGG 0.566969 4.4:+39622740 MS.gene003905:CDS
CAGGGAAGGTGCGAATGTCA+AGG 0.568897 4.4:+39623913 MS.gene003905:intron
CATGCAGAAAAGATCAAGGA+TGG 0.572632 4.4:+39621928 MS.gene003905:intron
AATCGGAATAGTTTCCCTCA+AGG 0.572952 4.4:+39621581 MS.gene003905:CDS
TTTGAAGGTGCAGCAACAGA+AGG 0.574188 4.4:+39621763 MS.gene003905:CDS
TGGATACTCACACTATAAGA+GGG 0.574493 4.4:-39622562 None:intergenic
GCAAATGGGCGAATGGCACC+GGG 0.577373 4.4:+39622776 MS.gene003905:CDS
GTATTGATGATATGTGTCAA+TGG 0.577465 4.4:-39621968 None:intergenic
TGGCTATGCAAATGGGCGAA+TGG 0.578351 4.4:+39622769 MS.gene003905:CDS
CTCGATCGCAGAATCACCTC+CGG 0.582972 4.4:-39622831 None:intergenic
GGTTAACAATGGTGCATCAG+AGG 0.590321 4.4:-39623288 None:intergenic
TGATTCTACATTATCAGTTG+AGG 0.592151 4.4:+39623728 MS.gene003905:CDS
AACCAACTAGAGCAACGACC+CGG 0.592189 4.4:-39622794 None:intergenic
AGGGAAGGTGCGAATGTCAA+GGG 0.596236 4.4:+39623914 MS.gene003905:intron
AAGTACAAGAAATCTCAAAA+GGG 0.596464 4.4:+39622917 MS.gene003905:intron
TCGGATTTGGAATTTGAGAG+TGG 0.600864 4.4:-39622977 None:intergenic
AGTGGTGGTGTCAATCAAGA+AGG 0.603295 4.4:+39622324 MS.gene003905:CDS
GTGAATGTGTCCCATATACT+TGG 0.603407 4.4:-39621796 None:intergenic
TGAATTGCAGTTGAGCGCAA+CGG 0.604196 4.4:+39623450 MS.gene003905:intron
TAAGTAACTAGGTCTAGCAT+TGG 0.612411 4.4:-39623309 None:intergenic
ACTTGGCAATATTCTAGACC+AGG 0.618905 4.4:-39622173 None:intergenic
GAAGGTGCAGCAACAGAAGG+TGG 0.627337 4.4:+39621766 MS.gene003905:CDS
TTGAATCCAAATTGTACCGG+AGG 0.632737 4.4:+39622815 MS.gene003905:CDS
AAGTTTGAGGGAGATCCCAA+TGG 0.634673 4.4:-39622511 None:intergenic
TGGTTGTACGTTTATCCAAG+AGG 0.645166 4.4:+39623504 MS.gene003905:CDS
AAAAGCATGAGAGCCCTCGT+TGG 0.645860 4.4:+39623173 MS.gene003905:CDS
GCCATTCTTATAGTCGACAA+AGG 0.646051 4.4:-39624005 None:intergenic
GGTCTAGCATTGGTTAACAA+TGG 0.647381 4.4:-39623299 None:intergenic
GCCCCAAAGATAAAGCCTTG+AGG 0.657999 4.4:-39621596 None:intergenic
ACAGATGGAAAGCTTAGTGG+TGG 0.663853 4.4:+39622309 MS.gene003905:intron
TGACACATATCATCAATACA+AGG 0.667772 4.4:+39621972 MS.gene003905:CDS
ACTTTAGAAGAAGAGTATGG+TGG 0.678058 4.4:+39622530 MS.gene003905:CDS
TGGTAATCTAGCACCAACGA+GGG 0.684546 4.4:-39623186 None:intergenic
TTGGTAATCTAGCACCAACG+AGG 0.689118 4.4:-39623187 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAAATTTATTTATTTC+AGG + chr4.4:39623117-39623136 MS.gene003905:intron 10.0%
!! AATTGATTATCAAATATTAT+AGG + chr4.4:39622464-39622483 MS.gene003905:intron 10.0%
!! AATTTATTTAATTAATTACC+TGG - chr4.4:39621637-39621656 None:intergenic 10.0%
!!! TTTTTTAAGATACATAAAAA+TGG - chr4.4:39623069-39623088 None:intergenic 10.0%
!! AAAAAAAAATGACAAATTTG+TGG + chr4.4:39623083-39623102 MS.gene003905:intron 15.0%
!! TAAATTATAGAGTTGTAAAA+TGG - chr4.4:39623373-39623392 None:intergenic 15.0%
!!! AAAACTATGATTTTATATAC+AGG - chr4.4:39623611-39623630 None:intergenic 15.0%
!!! ATTTATTTATTTCAGGTTTA+TGG + chr4.4:39623124-39623143 MS.gene003905:intron 15.0%
!! AATTAATTTATCCAAACAGA+TGG + chr4.4:39622294-39622313 MS.gene003905:intron 20.0%
!! AATTCATCACGAAAAAAAAA+AGG - chr4.4:39623436-39623455 None:intergenic 20.0%
!! AATTTACAATTTGTACAACT+TGG + chr4.4:39621900-39621919 MS.gene003905:intron 20.0%
!! ACTTCAATAATTATGTGTAT+AGG + chr4.4:39622017-39622036 MS.gene003905:intron 20.0%
!! ATTCATCACGAAAAAAAAAA+GGG - chr4.4:39623435-39623454 None:intergenic 20.0%
!! CCATTTGATTATATATTACA+GGG + chr4.4:39623895-39623914 MS.gene003905:intron 20.0%
!! TATTTAATTAATTACCTGGT+AGG - chr4.4:39621633-39621652 None:intergenic 20.0%
!! TCCATTTGATTATATATTAC+AGG + chr4.4:39623894-39623913 MS.gene003905:intron 20.0%
!!! AATTCAATGTATTTTGACAA+TGG + chr4.4:39623406-39623425 MS.gene003905:intron 20.0%
!!! ATTCTGCATATTTTAATTGT+AGG + chr4.4:39623690-39623709 MS.gene003905:intron 20.0%
!!! ATTGGTTCATTTGATTTTAT+TGG + chr4.4:39623239-39623258 MS.gene003905:CDS 20.0%
!!! TTGTTGTTCATTTGTATTAT+AGG + chr4.4:39622096-39622115 MS.gene003905:intron 20.0%
! AAAGAATCTGTTAAGTAACT+AGG - chr4.4:39623323-39623342 None:intergenic 25.0%
! AAAGTACAAGAAATCTCAAA+AGG + chr4.4:39622916-39622935 MS.gene003905:intron 25.0%
! AAGATTTATTTGTTCATACC+AGG - chr4.4:39621832-39621851 None:intergenic 25.0%
! AAGTACAAGAAATCTCAAAA+GGG + chr4.4:39622917-39622936 MS.gene003905:intron 25.0%
! AGTCAAGTCAATATATATGA+TGG + chr4.4:39622197-39622216 MS.gene003905:intron 25.0%
! CAAAAGTAATAAAGAAGAGA+AGG - chr4.4:39621481-39621500 None:intergenic 25.0%
! CCCTGTAATATATAATCAAA+TGG - chr4.4:39623898-39623917 None:intergenic 25.0%
! GATAAAATAGATGACGATAA+TGG - chr4.4:39623785-39623804 None:intergenic 25.0%
! TAGTTACTTAACAGATTCTT+TGG + chr4.4:39623322-39623341 MS.gene003905:CDS 25.0%
! TATAGAGTTGTAAAATGGAT+TGG - chr4.4:39623368-39623387 None:intergenic 25.0%
! TATTGGTATTAAGATTGCTT+CGG + chr4.4:39623479-39623498 MS.gene003905:CDS 25.0%
! TCTATTTACATCACAGAAAA+TGG + chr4.4:39623573-39623592 MS.gene003905:CDS 25.0%
! TTGATTATATATTACAGGGA+AGG + chr4.4:39623899-39623918 MS.gene003905:intron 25.0%
! TTGTTCATCATACATGTTAA+TGG + chr4.4:39623857-39623876 MS.gene003905:intron 25.0%
!! AGGATTGTTGTATTTTTCTT+TGG - chr4.4:39623553-39623572 None:intergenic 25.0%
!! ATAAAGTACTACTTACCTAA+TGG - chr4.4:39623817-39623836 None:intergenic 25.0%
!! CTATGATTTTATATACAGGT+AGG - chr4.4:39623607-39623626 None:intergenic 25.0%
!! GAAGACAATAAACAATGTTT+TGG - chr4.4:39622585-39622604 None:intergenic 25.0%
!! GTTTGCTTTGTATTGAATTT+TGG - chr4.4:39623209-39623228 None:intergenic 25.0%
!! TTTTCTGTGATGTAAATAGA+AGG - chr4.4:39623573-39623592 None:intergenic 25.0%
!!! AAAAGATTTTCAAGACTATG+CGG + chr4.4:39622670-39622689 MS.gene003905:CDS 25.0%
!!! AGAGCAATTGATTTTATGTA+TGG + chr4.4:39623016-39623035 MS.gene003905:CDS 25.0%
!!! ATATATTGACTTGACTTACT+TGG - chr4.4:39622193-39622212 None:intergenic 25.0%
!!! CAATTGATTTTATGTATGGA+TGG + chr4.4:39623020-39623039 MS.gene003905:CDS 25.0%
!!! TGTTTGTATGATTAGTTTGA+AGG + chr4.4:39621748-39621767 MS.gene003905:intron 25.0%
AAGCAAACTAAACTACTCAT+TGG + chr4.4:39623221-39623240 MS.gene003905:CDS 30.0%
AATGAAGCAATGTCAAGAAT+AGG - chr4.4:39621560-39621579 None:intergenic 30.0%
ATGTCAAGGGTTATTATGTA+TGG + chr4.4:39623927-39623946 MS.gene003905:CDS 30.0%
GAAGCAATCTTAATACCAAT+AGG - chr4.4:39623480-39623499 None:intergenic 30.0%
TGACACATATCATCAATACA+AGG + chr4.4:39621972-39621991 MS.gene003905:CDS 30.0%
TGATTCTACATTATCAGTTG+AGG + chr4.4:39623728-39623747 MS.gene003905:CDS 30.0%
TTGACATTGCTTCATTGAAT+CGG + chr4.4:39621564-39621583 MS.gene003905:CDS 30.0%
TTTCAACAGGTCATAGAAAA+TGG - chr4.4:39623648-39623667 None:intergenic 30.0%
! ATTAGTGACTCTTTTCCATT+GGG + chr4.4:39622496-39622515 MS.gene003905:CDS 30.0%
! CAAACTTTAGAAGAAGAGTA+TGG + chr4.4:39622527-39622546 MS.gene003905:CDS 30.0%
! CATACACAATTGATTTCAAC+AGG - chr4.4:39623661-39623680 None:intergenic 30.0%
! CATTGTTCGACAATTTTGAA+TGG + chr4.4:39623951-39623970 MS.gene003905:CDS 30.0%
! GAACAATAGAGAACTCTTTT+GGG - chr4.4:39623843-39623862 None:intergenic 30.0%
! GAACTTTGTTTCAAGGAATT+TGG + chr4.4:39622692-39622711 MS.gene003905:CDS 30.0%
! GTATTGATGATATGTGTCAA+TGG - chr4.4:39621971-39621990 None:intergenic 30.0%
! TGAACAATAGAGAACTCTTT+TGG - chr4.4:39623844-39623863 None:intergenic 30.0%
!! ACTCTTCTTCTAAAGTTTGA+GGG - chr4.4:39622526-39622545 None:intergenic 30.0%
!! TACTCTTCTTCTAAAGTTTG+AGG - chr4.4:39622527-39622546 None:intergenic 30.0%
AAAAGATCACGAATTCCTCT+TGG - chr4.4:39623522-39623541 None:intergenic 35.0%
AAGATACCAATTCGCTACTA+AGG - chr4.4:39622957-39622976 None:intergenic 35.0%
ACATTCACTCATAGATATCC+TGG + chr4.4:39621811-39621830 MS.gene003905:CDS 35.0%
CAATATTCTAGACCAGGATA+TGG - chr4.4:39622170-39622189 None:intergenic 35.0%
CAATGGAAAAGAGTCACTAA+TGG - chr4.4:39622497-39622516 None:intergenic 35.0%
GTATTAAGATTGCTTCGGAA+TGG + chr4.4:39623484-39623503 MS.gene003905:CDS 35.0%
TAAGTAACTAGGTCTAGCAT+TGG - chr4.4:39623312-39623331 None:intergenic 35.0%
TCCAAATCTGACACTATAAC+CGG - chr4.4:39623981-39624000 None:intergenic 35.0%
TGGATACTCACACTATAAGA+GGG - chr4.4:39622565-39622584 None:intergenic 35.0%
TGGTAGAACACCAAGTATAT+GGG + chr4.4:39621786-39621805 MS.gene003905:CDS 35.0%
TGGTGATAGAGTCAAGTATT+GGG + chr4.4:39622712-39622731 MS.gene003905:CDS 35.0%
TGGTTGAATCCAAATTGTAC+CGG + chr4.4:39622812-39622831 MS.gene003905:CDS 35.0%
TTGACATGCAGAAAAGATCA+AGG + chr4.4:39621924-39621943 MS.gene003905:intron 35.0%
TTGGATACTCACACTATAAG+AGG - chr4.4:39622566-39622585 None:intergenic 35.0%
TTTATCTTCGATCTGCCATT+AGG + chr4.4:39623799-39623818 MS.gene003905:CDS 35.0%
TTTATGGATCCACTAACATC+AGG + chr4.4:39623140-39623159 MS.gene003905:CDS 35.0%
! AAATCTCAAAAGGGCGTAAT+AGG + chr4.4:39622926-39622945 MS.gene003905:CDS 35.0%
! ACCTTTGTCGACTATAAGAA+TGG + chr4.4:39624004-39624023 MS.gene003905:CDS 35.0%
! ACTTTAGAAGAAGAGTATGG+TGG + chr4.4:39622530-39622549 MS.gene003905:CDS 35.0%
! CATACAGATTTTCCATATCC+TGG + chr4.4:39622155-39622174 MS.gene003905:CDS 35.0%
! CATTAGTGACTCTTTTCCAT+TGG + chr4.4:39622495-39622514 MS.gene003905:CDS 35.0%
! GACAATTTTGAATGGTCATC+CGG + chr4.4:39623959-39623978 MS.gene003905:CDS 35.0%
! TGGTAATGCGTAACCAAATA+GGG - chr4.4:39622857-39622876 None:intergenic 35.0%
! TTGGTGATAGAGTCAAGTAT+TGG + chr4.4:39622711-39622730 MS.gene003905:CDS 35.0%
!! TTGGTAATGCGTAACCAAAT+AGG - chr4.4:39622858-39622877 None:intergenic 35.0%
AAGATCAAGGATGGAAGCAA+TGG + chr4.4:39621937-39621956 MS.gene003905:CDS 40.0%
AATCGGAATAGTTTCCCTCA+AGG + chr4.4:39621581-39621600 MS.gene003905:CDS 40.0%
ACTTGGCAATATTCTAGACC+AGG - chr4.4:39622176-39622195 None:intergenic 40.0%
ATCAACGAGCTACTAGCTAA+TGG + chr4.4:39622369-39622388 MS.gene003905:CDS 40.0%
ATCAGTTGAGGAATCACTCT+TGG + chr4.4:39623740-39623759 MS.gene003905:CDS 40.0%
CAAACAGATGGAAAGCTTAG+TGG + chr4.4:39622306-39622325 MS.gene003905:intron 40.0%
CACTAAGCTTTCCATCTGTT+TGG - chr4.4:39622308-39622327 None:intergenic 40.0%
CATGCAGAAAAGATCAAGGA+TGG + chr4.4:39621928-39621947 MS.gene003905:intron 40.0%
CTTGTACACATCAACAACAG+CGG - chr4.4:39622894-39622913 None:intergenic 40.0%
GCATAACCTTAGTAGCGAAT+TGG + chr4.4:39622948-39622967 MS.gene003905:CDS 40.0%
GCCAAAATGGCTATGCAAAT+GGG + chr4.4:39622762-39622781 MS.gene003905:CDS 40.0%
GCCATTCTTATAGTCGACAA+AGG - chr4.4:39624008-39624027 None:intergenic 40.0%
GGATAATCTCCTGATGTTAG+TGG - chr4.4:39623152-39623171 None:intergenic 40.0%
GTGAATGTGTCCCATATACT+TGG - chr4.4:39621799-39621818 None:intergenic 40.0%
GTGGTAGAACACCAAGTATA+TGG + chr4.4:39621785-39621804 MS.gene003905:CDS 40.0%
TCCGGTTATAGTGTCAGATT+TGG + chr4.4:39623977-39623996 MS.gene003905:CDS 40.0%
TGGTTGTACGTTTATCCAAG+AGG + chr4.4:39623504-39623523 MS.gene003905:CDS 40.0%
TTGAATCCAAATTGTACCGG+AGG + chr4.4:39622815-39622834 MS.gene003905:CDS 40.0%
! CTATGCGGAACTTTGTTTCA+AGG + chr4.4:39622685-39622704 MS.gene003905:CDS 40.0%
! TCCCTCAAGGCTTTATCTTT+GGG + chr4.4:39621594-39621613 MS.gene003905:CDS 40.0%
! TCGGATTTGGAATTTGAGAG+TGG - chr4.4:39622980-39622999 None:intergenic 40.0%
! TTCCCTCAAGGCTTTATCTT+TGG + chr4.4:39621593-39621612 MS.gene003905:CDS 40.0%
!! GGTCTAGCATTGGTTAACAA+TGG - chr4.4:39623302-39623321 None:intergenic 40.0%
!!! TCTTTCTGCTGCTTTTTGGT+CGG - chr4.4:39622999-39623018 None:intergenic 40.0%
!!! TTGCTCTTTCTGCTGCTTTT+TGG - chr4.4:39623003-39623022 None:intergenic 40.0%
AAGTTTGAGGGAGATCCCAA+TGG - chr4.4:39622514-39622533 None:intergenic 45.0%
ACAGATGGAAAGCTTAGTGG+TGG + chr4.4:39622309-39622328 MS.gene003905:intron 45.0%
CCCCAAAGATAAAGCCTTGA+GGG - chr4.4:39621598-39621617 None:intergenic 45.0%
GAATCACCTCCGGTACAATT+TGG - chr4.4:39622824-39622843 None:intergenic 45.0%
GGCCAAAATGGCTATGCAAA+TGG + chr4.4:39622761-39622780 MS.gene003905:CDS 45.0%
GGTTAACAATGGTGCATCAG+AGG - chr4.4:39623291-39623310 None:intergenic 45.0%
TGAATTGCAGTTGAGCGCAA+CGG + chr4.4:39623450-39623469 MS.gene003905:intron 45.0%
TGGTAATCTAGCACCAACGA+GGG - chr4.4:39623189-39623208 None:intergenic 45.0%
TTGAATGAGCCATGGAGCTA+CGG + chr4.4:39622740-39622759 MS.gene003905:CDS 45.0%
! AGTGGTGGTGTCAATCAAGA+AGG + chr4.4:39622324-39622343 MS.gene003905:CDS 45.0%
! CCCTCAAGGCTTTATCTTTG+GGG + chr4.4:39621595-39621614 MS.gene003905:CDS 45.0%
! GCCCATTTGCATAGCCATTT+TGG - chr4.4:39622766-39622785 None:intergenic 45.0%
! GGGTTACTTTGAATGAGCCA+TGG + chr4.4:39622732-39622751 MS.gene003905:CDS 45.0%
! TTGGTAATCTAGCACCAACG+AGG - chr4.4:39623190-39623209 None:intergenic 45.0%
! TTTGAAGGTGCAGCAACAGA+AGG + chr4.4:39621763-39621782 MS.gene003905:CDS 45.0%
!! TGGATTGGTTGCTTACATGC+TGG - chr4.4:39623353-39623372 None:intergenic 45.0%
!!! TGCTGCTTTTTGGTCGGATT+TGG - chr4.4:39622993-39623012 None:intergenic 45.0%
!!! ATAAATTTGAATTAAATTTT+TGG - chr4.4:39623110-39623129 None:intergenic 5.0%
AACCAACTAGAGCAACGACC+CGG - chr4.4:39622797-39622816 None:intergenic 50.0%
GAATTGCAGTTGAGCGCAAC+GGG + chr4.4:39623451-39623470 MS.gene003905:intron 50.0%
GCCCCAAAGATAAAGCCTTG+AGG - chr4.4:39621599-39621618 None:intergenic 50.0%
TGGCTATGCAAATGGGCGAA+TGG + chr4.4:39622769-39622788 MS.gene003905:CDS 50.0%
TTCTGCGATCGAGCCCTATT+TGG + chr4.4:39622841-39622860 MS.gene003905:CDS 50.0%
! AAAAGCATGAGAGCCCTCGT+TGG + chr4.4:39623173-39623192 MS.gene003905:CDS 50.0%
! ACGAGGGCTCTCATGCTTTT+AGG - chr4.4:39623173-39623192 None:intergenic 50.0%
! AGGGAAGGTGCGAATGTCAA+GGG + chr4.4:39623914-39623933 MS.gene003905:intron 50.0%
!! CAAGGCTTTATCTTTGGGGC+TGG + chr4.4:39621599-39621618 MS.gene003905:CDS 50.0%
CAGCGGCATGTGCAAGAAGT+TGG - chr4.4:39622877-39622896 None:intergenic 55.0%
CCATGGAGCTACGGCCAAAA+TGG + chr4.4:39622749-39622768 MS.gene003905:CDS 55.0%
CTCGATCGCAGAATCACCTC+CGG - chr4.4:39622834-39622853 None:intergenic 55.0%
GAGCGCAACGGGAAACCTAT+TGG + chr4.4:39623462-39623481 MS.gene003905:CDS 55.0%
TGCAAATGGGCGAATGGCAC+CGG + chr4.4:39622775-39622794 MS.gene003905:CDS 55.0%
! CAGGGAAGGTGCGAATGTCA+AGG + chr4.4:39623913-39623932 MS.gene003905:intron 55.0%
! GAAGGTGCAGCAACAGAAGG+TGG + chr4.4:39621766-39621785 MS.gene003905:CDS 55.0%
! GGCTGGTTCATCTTCCTACC+AGG + chr4.4:39621616-39621635 MS.gene003905:CDS 55.0%
!! CCATTTTGGCCGTAGCTCCA+TGG - chr4.4:39622752-39622771 None:intergenic 55.0%
CACCGGGTCGTTGCTCTAGT+TGG + chr4.4:39622792-39622811 MS.gene003905:CDS 60.0%
GCAAATGGGCGAATGGCACC+GGG + chr4.4:39622776-39622795 MS.gene003905:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 39621464 39624047 39621464 ID=MS.gene003905
chr4.4 mRNA 39621464 39624047 39621464 ID=MS.gene003905.t1;Parent=MS.gene003905
chr4.4 exon 39621464 39621637 39621464 ID=MS.gene003905.t1.exon1;Parent=MS.gene003905.t1
chr4.4 CDS 39621464 39621637 39621464 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39621763 39621832 39621763 ID=MS.gene003905.t1.exon2;Parent=MS.gene003905.t1
chr4.4 CDS 39621763 39621832 39621763 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39621935 39621993 39621935 ID=MS.gene003905.t1.exon3;Parent=MS.gene003905.t1
chr4.4 CDS 39621935 39621993 39621935 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39622118 39622193 39622118 ID=MS.gene003905.t1.exon4;Parent=MS.gene003905.t1
chr4.4 CDS 39622118 39622193 39622118 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39622313 39622390 39622313 ID=MS.gene003905.t1.exon5;Parent=MS.gene003905.t1
chr4.4 CDS 39622313 39622390 39622313 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39622486 39622573 39622486 ID=MS.gene003905.t1.exon6;Parent=MS.gene003905.t1
chr4.4 CDS 39622486 39622573 39622486 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39622670 39622895 39622670 ID=MS.gene003905.t1.exon7;Parent=MS.gene003905.t1
chr4.4 CDS 39622670 39622895 39622670 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39622926 39623041 39622926 ID=MS.gene003905.t1.exon8;Parent=MS.gene003905.t1
chr4.4 CDS 39622926 39623041 39622926 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39623139 39623356 39623139 ID=MS.gene003905.t1.exon9;Parent=MS.gene003905.t1
chr4.4 CDS 39623139 39623356 39623139 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39623460 39623594 39623460 ID=MS.gene003905.t1.exon10;Parent=MS.gene003905.t1
chr4.4 CDS 39623460 39623594 39623460 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39623712 39623820 39623712 ID=MS.gene003905.t1.exon11;Parent=MS.gene003905.t1
chr4.4 CDS 39623712 39623820 39623712 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
chr4.4 exon 39623916 39624047 39623916 ID=MS.gene003905.t1.exon12;Parent=MS.gene003905.t1
chr4.4 CDS 39623916 39624047 39623916 ID=cds.MS.gene003905.t1;Parent=MS.gene003905.t1
Gene Sequence

>MS.gene003905

ATGGCATTCAATTACCTTCTCTTCTTTATTACTTTTGTTATTTTTCTTAGCAAATCAAATATTGTGAATTGCACAGAAAGTGTTGAAGTTGAACCTATTCTTGACATTGCTTCATTGAATCGGAATAGTTTCCCTCAAGGCTTTATCTTTGGGGCTGGTTCATCTTCCTACCAGGTAATTAATTAAATAAATTATAATCAACTTTTTACAATATATTAATTAACATATTATACATATATATATGATATTATTGTTTTGATTGTGTTGTTTCTAACATGTGTGTATGTTTGTATGATTAGTTTGAAGGTGCAGCAACAGAAGGTGGTAGAACACCAAGTATATGGGACACATTCACTCATAGATATCCTGGTATGAACAAATAAATCTTTCTATTTTTCAATTTACATAAGAAACACAATTTTACAATTTGATATATAATTTACAATTTGTACAACTTGGATTGACATGCAGAAAAGATCAAGGATGGAAGCAATGGAGATGTAGCCATTGACACATATCATCAATACAAGGTAATTAATTATGTGATTTTCAAACTTCAATAATTATGTGTATAGGTTTTTTAGTAATTAATTTTGATTTTGTGTTTTGATTTAGTAAAATAATTTAATATCTTGTTGTTCATTTGTATTATAGGAAGATGTTAAGATCATGAAAGATATGAACTTAGATTCATACAGATTTTCCATATCCTGGTCTAGAATATTGCCAAGTAAGTCAAGTCAATATATATGATGGATATTCTCAACTAATTTTTATCGTAATAGAGATGTTTAACTTAATTTAAATATTTATCAATATCTTTTTATAATAATTAATTTATCCAAACAGATGGAAAGCTTAGTGGTGGTGTCAATCAAGAAGGAATTAACTATTACAACAATCTCATCAACGAGCTACTAGCTAATGGTTAGAAATGATTCATACGTTTATTTCACTAACTTATTTTCATATATATGTTTGTTGATGAAATAACATAATTAATTGATTATCAAATATTATAGGTATACAACCATTAGTGACTCTTTTCCATTGGGATCTCCCTCAAACTTTAGAAGAAGAGTATGGTGGCTTCTTAAGCCCTCTTATAGTGTGAGTATCCAAAACATTGTTTATTGTCTTCATAATATATATACACTATATATTTAAATTTGATTTTGATTTGTTACATGTTTCTTAAATTTTCAGAAAAGATTTTCAAGACTATGCGGAACTTTGTTTCAAGGAATTTGGTGATAGAGTCAAGTATTGGGTTACTTTGAATGAGCCATGGAGCTACGGCCAAAATGGCTATGCAAATGGGCGAATGGCACCGGGTCGTTGCTCTAGTTGGTTGAATCCAAATTGTACCGGAGGTGATTCTGCGATCGAGCCCTATTTGGTTACGCATTACCAACTTCTTGCACATGCCGCTGTTGTTGATGTGTACAAGAAAAAGTACAAGAAATCTCAAAAGGGCGTAATAGGCATAACCTTAGTAGCGAATTGGTATCTTCCACTCTCAAATTCCAAATCCGACCAAAAAGCAGCAGAAAGAGCAATTGATTTTATGTATGGATGGTAAGAGCTCATCTATAACTCTTTCCATTTTTATGTATCTTAAAAAAAAATGACAAATTTGTGGACCAAAAATTTAATTCAAATTTATTTATTTCAGGTTTATGGATCCACTAACATCAGGAGATTATCCTAAAAGCATGAGAGCCCTCGTTGGTGCTAGATTACCAAAATTCAATACAAAGCAAACTAAACTACTCATTGGTTCATTTGATTTTATTGGTCTAAACTATTACTCTTCAACTTATGCCTCTGATGCACCATTGTTAACCAATGCTAGACCTAGTTACTTAACAGATTCTTTGGTCAATCCAGCATGTAAGCAACCAATCCATTTTACAACTCTATAATTTACTGAAACACTTTAAATTCAATGTATTTTGACAATGGATACCCTTTTTTTTTTCGTGATGAATTGCAGTTGAGCGCAACGGGAAACCTATTGGTATTAAGATTGCTTCGGAATGGTTGTACGTTTATCCAAGAGGAATTCGTGATCTTTTGTTATACACCAAAGAAAAATACAACAATCCTTCTATTTACATCACAGAAAATGGTAAAAACACCTACCTGTATATAAAATCATAGTTTTCTTGTCTTGAATTACCATTTTCTATGACCTGTTGAAATCAATTGTGTATGACAATTTTTATTCTGCATATTTTAATTGTAGGTATAAATGAATACGATGATTCTACATTATCAGTTGAGGAATCACTCTTGGATACTTTTCGAATTGATTACCATTATCGTCATCTATTTTATCTTCGATCTGCCATTAGGTAAGTAGTACTTTATCTTCCCAAAAGAGTTCTCTATTGTTCATCATACATGTTAATGGTTTTCTAAATTATGTCCATTTGATTATATATTACAGGGAAGGTGCGAATGTCAAGGGTTATTATGTATGGTCATTGTTCGACAATTTTGAATGGTCATCCGGTTATAGTGTCAGATTTGGAATGACCTTTGTCGACTATAAGAATGGCTTGAAAAGATACCAGAAGTT
Protein sequence

>MS.gene003905.t1

MAFNYLLFFITFVIFLSKSNIVNCTESVEVEPILDIASLNRNSFPQGFIFGAGSSSYQFEGAATEGGRTPSIWDTFTHRYPEKIKDGSNGDVAIDTYHQYKEDVKIMKDMNLDSYRFSISWSRILPNGKLSGGVNQEGINYYNNLINELLANGIQPLVTLFHWDLPQTLEEEYGGFLSPLIVKDFQDYAELCFKEFGDRVKYWVTLNEPWSYGQNGYANGRMAPGRCSSWLNPNCTGGDSAIEPYLVTHYQLLAHAAKSQKGVIGITLVANWYLPLSNSKSDQKAAERAIDFMYGWFMDPLTSGDYPKSMRALVGARLPKFNTKQTKLLIGSFDFIGLNYYSSTYASDAPLLTNARPSYLTDSLVNPAFERNGKPIGIKIASEWLYVYPRGIRDLLLYTKEKYNNPSIYITENGINEYDDSTLSVEESLLDTFRIDYHYRHLFYLRSAIREGANVKGYYVWSLFDNFEWSSGYSVRFGMTFVDYKNGLKRYQK