AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003916

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003916.t1 MTR_4g066210 97.419 465 12 0 21 485 63 527 0.0 951
MS.gene003916.t1 MTR_4g066240 87.773 458 56 0 21 478 56 513 0.0 845
MS.gene003916.t1 MTR_4g066240 87.582 459 56 1 21 478 56 514 0.0 840
MS.gene003916.t1 MTR_4g066340 79.080 435 91 0 44 478 6 440 0.0 732
MS.gene003916.t1 MTR_4g066580 68.696 460 144 0 21 480 59 518 0.0 694
MS.gene003916.t1 MTR_4g066630 68.791 455 142 0 21 475 59 513 0.0 685
MS.gene003916.t1 MTR_3g028720 67.174 460 151 0 21 480 58 517 0.0 684
MS.gene003916.t1 MTR_2g098790 68.709 457 143 0 21 477 58 514 0.0 684
MS.gene003916.t1 MTR_2g098520 67.177 457 150 0 21 477 54 510 0.0 681
MS.gene003916.t1 MTR_4g066590 67.028 461 152 0 21 481 53 513 0.0 679
MS.gene003916.t1 MTR_4g066240 87.193 367 47 0 21 387 56 422 0.0 677
MS.gene003916.t1 MTR_6g078450 66.449 459 154 0 21 479 56 514 0.0 676
MS.gene003916.t1 MTR_2g098540 66.740 457 152 0 21 477 51 507 0.0 673
MS.gene003916.t1 MTR_2g098540 66.234 462 156 0 16 477 2 463 0.0 673
MS.gene003916.t1 MTR_6g078330 67.396 457 149 0 21 477 57 513 0.0 667
MS.gene003916.t1 MTR_6g078380 68.928 457 141 1 21 476 56 512 0.0 659
MS.gene003916.t1 MTR_4g066640 64.270 459 160 2 21 478 59 514 0.0 644
MS.gene003916.t1 MTR_5g071520 63.948 466 159 1 21 477 59 524 0.0 616
MS.gene003916.t1 MTR_4g066330 62.745 459 143 2 21 477 61 493 0.0 608
MS.gene003916.t1 MTR_4g066630 68.828 401 125 0 21 421 59 459 0.0 605
MS.gene003916.t1 MTR_3g028720 67.662 402 130 0 21 422 58 459 0.0 603
MS.gene003916.t1 MTR_4g066280 61.656 459 148 2 21 477 61 493 0.0 602
MS.gene003916.t1 MTR_6g078330 67.482 409 133 0 69 477 1 409 0.0 599
MS.gene003916.t1 MTR_6g078450 67.413 402 131 0 21 422 56 457 0.0 595
MS.gene003916.t1 MTR_3g026280 59.300 457 181 2 21 477 42 493 0.0 580
MS.gene003916.t1 MTR_6g078330 65.995 397 133 1 81 477 15 409 0.0 563
MS.gene003916.t1 MTR_4g066580 68.649 370 116 0 21 390 59 428 0.0 561
MS.gene003916.t1 MTR_4g066630 68.937 367 114 0 21 387 59 425 0.0 558
MS.gene003916.t1 MTR_3g028720 67.935 368 118 0 21 388 58 425 0.0 554
MS.gene003916.t1 MTR_4g023000 57.330 457 189 3 21 477 44 494 0.0 547
MS.gene003916.t1 MTR_4g067040 67.797 354 114 0 21 374 54 407 0.0 529
MS.gene003916.t1 MTR_2g043200 56.803 463 166 5 21 477 85 519 0.0 525
MS.gene003916.t1 MTR_4g015420 53.945 469 176 6 21 477 85 525 0.0 523
MS.gene003916.t1 MTR_3g026400 53.611 457 206 4 22 477 27 478 0.0 515
MS.gene003916.t1 MTR_3g026400 53.611 457 206 4 22 477 58 509 1.21e-180 516
MS.gene003916.t1 MTR_4g067040 69.605 329 100 0 21 349 54 382 5.22e-179 507
MS.gene003916.t1 MTR_3g028720 68.389 329 104 0 21 349 58 386 8.83e-178 504
MS.gene003916.t1 MTR_4g015460 53.961 467 176 9 21 476 62 500 1.15e-174 500
MS.gene003916.t1 MTR_4g066630 70.126 318 95 0 21 338 59 376 7.80e-174 494
MS.gene003916.t1 MTR_3g026620 53.509 456 208 3 22 477 59 510 2.65e-173 497
MS.gene003916.t1 MTR_4g083000 51.983 479 216 6 4 476 32 502 7.44e-172 493
MS.gene003916.t1 MTR_5g044000 52.838 458 210 4 21 475 66 520 1.21e-169 488
MS.gene003916.t1 MTR_6g078330 67.812 320 103 0 21 340 57 376 6.46e-165 471
MS.gene003916.t1 MTR_4g066640 62.840 331 119 2 21 350 59 386 9.42e-159 456
MS.gene003916.t1 MTR_0110s0040 49.672 457 221 6 22 477 64 512 4.24e-155 451
MS.gene003916.t1 MTR_0110s0030 48.718 468 229 7 13 477 53 512 5.82e-155 451
MS.gene003916.t1 MTR_4g081530 48.643 479 237 6 10 484 34 507 1.02e-154 450
MS.gene003916.t1 MTR_4g131810 49.127 458 228 5 21 475 48 503 1.67e-154 450
MS.gene003916.t1 MTR_2g022760 49.028 463 231 5 21 481 56 515 3.92e-151 441
MS.gene003916.t1 MTR_4g083000 51.691 414 188 4 69 476 1 408 2.65e-149 432
MS.gene003916.t1 MTR_3g033790 45.570 474 238 7 13 482 29 486 2.17e-144 423
MS.gene003916.t1 MTR_3g033810 45.895 475 236 7 13 482 31 489 9.39e-144 421
MS.gene003916.t1 MTR_4g131800 48.164 463 234 6 21 481 536 994 1.20e-143 437
MS.gene003916.t1 MTR_2g079550 45.043 464 244 6 22 481 51 507 1.38e-139 412
MS.gene003916.t1 MTR_2g079580 44.612 464 246 6 22 481 53 509 5.88e-139 410
MS.gene003916.t1 MTR_2g022710 43.791 459 252 5 21 477 55 509 1.12e-137 407
MS.gene003916.t1 MTR_7g068910 44.211 475 235 8 13 477 41 495 7.15e-137 405
MS.gene003916.t1 MTR_7g033860 45.435 460 236 5 22 479 69 515 1.05e-136 404
MS.gene003916.t1 MTR_3g026400 61.953 297 110 2 22 318 58 351 5.65e-134 392
MS.gene003916.t1 MTR_7g068910 43.299 485 235 9 13 477 41 505 1.29e-133 397
MS.gene003916.t1 MTR_5g068740 43.818 461 238 8 22 478 48 491 7.79e-130 387
MS.gene003916.t1 MTR_4g016920 43.441 465 245 7 22 483 48 497 8.36e-130 387
MS.gene003916.t1 MTR_2g022760 47.470 415 213 5 69 481 1 412 3.38e-128 379
MS.gene003916.t1 MTR_0108s0030 42.609 460 235 4 22 481 24 454 1.42e-127 379
MS.gene003916.t1 MTR_1g088610 43.573 459 237 7 22 476 48 488 3.05e-124 376
MS.gene003916.t1 MTR_1g088610 41.176 119 63 2 361 478 503 615 4.88e-18 87.4
MS.gene003916.t1 MTR_7g068900 45.305 426 197 9 64 476 17 419 1.29e-119 359
MS.gene003916.t1 MTR_1g088630 42.072 473 228 10 10 478 4 434 1.40e-119 358
MS.gene003916.t1 MTR_2g022710 42.857 406 227 4 74 477 6 408 5.90e-119 355
MS.gene003916.t1 MTR_7g051510 43.319 464 237 8 22 481 96 537 7.30e-117 354
MS.gene003916.t1 MTR_7g051610 43.384 461 239 6 17 475 91 531 4.31e-115 349
MS.gene003916.t1 MTR_2g079580 46.341 369 188 5 22 386 53 415 4.49e-115 345
MS.gene003916.t1 MTR_2g022710 42.327 404 228 4 21 422 55 455 1.22e-114 346
MS.gene003916.t1 MTR_2g022710 42.327 404 228 4 21 422 55 455 2.18e-114 345
MS.gene003916.t1 MTR_7g051650 41.667 456 245 5 22 475 96 532 3.17e-113 345
MS.gene003916.t1 MTR_4g016920 42.344 418 224 6 69 483 1 404 1.48e-112 339
MS.gene003916.t1 MTR_2g022710 43.902 369 202 4 21 387 55 420 3.38e-112 338
MS.gene003916.t1 MTR_0110s0040 52.396 313 145 3 22 334 64 372 3.96e-112 337
MS.gene003916.t1 MTR_7g051650 41.228 456 243 5 22 475 96 528 6.99e-112 341
MS.gene003916.t1 MTR_2g022710 43.258 356 197 4 21 374 55 407 2.05e-105 320
MS.gene003916.t1 MTR_7g056747 42.548 416 209 5 63 475 9 397 3.39e-104 317
MS.gene003916.t1 MTR_2g022710 48.173 301 153 3 21 321 55 352 9.87e-99 303
MS.gene003916.t1 MTR_1g088630 47.333 300 128 5 22 320 48 318 2.47e-92 285
MS.gene003916.t1 MTR_4g016920 51.190 252 121 1 69 320 1 250 1.46e-90 278
MS.gene003916.t1 MTR_1g088630 50.607 247 116 3 22 267 48 289 9.22e-82 256
MS.gene003916.t1 MTR_0110s0020 50.216 231 109 4 13 241 53 279 7.08e-75 238
MS.gene003916.t1 MTR_5g069350 41.341 179 52 5 315 477 48 189 1.02e-29 115
MS.gene003916.t1 MTR_4g122980 27.751 418 227 13 65 475 183 532 2.16e-29 122
MS.gene003916.t1 MTR_8g038650 46.000 100 54 0 227 326 57 156 1.18e-23 97.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003916.t1 AT5G44640 54.267 457 203 3 22 477 52 503 0.0 533
MS.gene003916.t1 AT2G44450 54.048 457 203 3 22 477 52 502 0.0 533
MS.gene003916.t1 AT5G42260 54.989 451 197 3 22 471 52 497 0.0 531
MS.gene003916.t1 AT2G44480 53.173 457 213 1 22 478 87 542 0.0 530
MS.gene003916.t1 AT2G44480 53.173 457 213 1 22 478 56 511 0.0 530
MS.gene003916.t1 AT3G60130 54.386 456 202 3 22 476 51 501 1.90e-180 516
MS.gene003916.t1 AT2G25630 53.187 455 190 4 22 475 51 483 2.89e-175 501
MS.gene003916.t1 AT5G24550 51.948 462 217 5 21 478 52 512 1.37e-173 499
MS.gene003916.t1 AT5G24540 52.165 462 216 5 21 478 52 512 1.92e-165 478
MS.gene003916.t1 AT3G60140 48.837 473 237 5 10 479 36 506 3.60e-164 476
MS.gene003916.t1 AT1G26560 48.918 462 228 6 21 478 52 509 4.01e-162 469
MS.gene003916.t1 AT2G44480 51.707 410 197 1 69 478 1 409 8.06e-162 464
MS.gene003916.t1 AT2G44460 51.398 465 219 6 21 481 49 510 6.28e-161 468
MS.gene003916.t1 AT1G51470 49.782 458 216 6 22 477 66 511 5.73e-160 463
MS.gene003916.t1 AT2G44470 47.789 475 235 6 10 477 39 507 7.05e-160 466
MS.gene003916.t1 AT5G54570 50.545 459 220 4 21 475 50 505 1.24e-159 463
MS.gene003916.t1 AT1G47600 49.351 462 212 7 22 477 66 511 2.70e-158 459
MS.gene003916.t1 AT3G60130 53.012 415 189 3 63 476 40 449 3.08e-158 457
MS.gene003916.t1 AT3G60120 50.108 463 220 7 21 477 34 491 1.18e-157 458
MS.gene003916.t1 AT1G47600 49.134 462 212 7 22 477 66 510 2.76e-157 456
MS.gene003916.t1 AT3G18080 49.237 459 223 7 22 478 60 510 4.03e-155 451
MS.gene003916.t1 AT2G44490 48.156 461 233 5 21 476 34 493 4.84e-153 447
MS.gene003916.t1 AT5G25980 48.164 463 223 10 29 482 75 529 4.22e-150 439
MS.gene003916.t1 AT3G60130 51.566 415 184 4 63 476 40 438 2.91e-149 434
MS.gene003916.t1 AT5G36890 48.203 473 230 5 13 482 26 486 7.53e-149 434
MS.gene003916.t1 AT5G36890 48.203 473 230 5 13 482 26 486 8.04e-149 434
MS.gene003916.t1 AT2G32860 45.188 478 257 5 10 483 104 580 1.42e-147 435
MS.gene003916.t1 AT3G18070 46.187 459 227 9 22 477 51 492 4.12e-147 430
MS.gene003916.t1 AT2G32860 45.188 478 256 6 10 483 104 579 2.85e-146 432
MS.gene003916.t1 AT5G26000 48.261 460 214 9 29 475 63 511 3.43e-146 429
MS.gene003916.t1 AT1G61820 47.835 462 235 5 21 479 50 508 3.99e-146 428
MS.gene003916.t1 AT3G18070 46.187 459 233 9 22 477 51 498 6.44e-146 427
MS.gene003916.t1 AT3G03640 45.238 462 247 4 21 477 54 514 6.44e-144 423
MS.gene003916.t1 AT5G54570 50.122 411 198 4 69 475 1 408 4.14e-141 412
MS.gene003916.t1 AT1G02850 46.667 465 221 8 22 480 47 490 2.78e-140 412
MS.gene003916.t1 AT3G09260 45.806 465 241 6 22 480 56 515 1.18e-139 412
MS.gene003916.t1 AT5G24540 50.743 404 195 4 21 421 52 454 2.41e-138 406
MS.gene003916.t1 AT1G75940 45.671 462 241 7 22 475 58 517 8.81e-138 407
MS.gene003916.t1 AT2G44470 47.368 418 208 5 10 421 39 450 6.00e-137 402
MS.gene003916.t1 AT5G54570 50.370 405 195 4 21 422 50 451 1.07e-136 402
MS.gene003916.t1 AT1G02850 44.856 486 218 9 22 478 47 511 1.24e-135 402
MS.gene003916.t1 AT1G66280 45.474 464 243 7 22 480 57 515 5.95e-135 400
MS.gene003916.t1 AT5G28510 43.617 470 249 7 22 480 60 524 1.49e-134 399
MS.gene003916.t1 AT3G21370 44.206 466 242 9 22 476 54 512 4.31e-134 398
MS.gene003916.t1 AT1G02850 44.764 487 218 10 22 478 47 512 8.13e-134 397
MS.gene003916.t1 AT2G44450 52.381 357 163 3 122 477 1 351 1.01e-133 390
MS.gene003916.t1 AT1G61810 43.656 465 256 5 21 482 53 514 2.13e-133 396
MS.gene003916.t1 AT4G21760 43.326 457 248 6 21 475 74 521 2.23e-133 397
MS.gene003916.t1 AT1G52400 45.238 462 241 9 22 476 60 516 6.25e-133 395
MS.gene003916.t1 AT1G52400 45.238 462 241 9 22 476 60 516 6.25e-133 395
MS.gene003916.t1 AT3G60140 48.564 383 193 4 10 390 36 416 1.85e-132 391
MS.gene003916.t1 AT1G66270 44.060 463 251 6 22 480 57 515 3.05e-132 393
MS.gene003916.t1 AT4G27830 42.333 463 246 7 21 479 43 488 5.17e-132 392
MS.gene003916.t1 AT5G25980 48.628 401 191 8 29 422 75 467 3.73e-131 388
MS.gene003916.t1 AT5G25980 48.628 401 191 8 29 422 75 467 3.73e-131 388
MS.gene003916.t1 AT1G02850 45.376 465 200 9 22 480 47 463 6.21e-131 388
MS.gene003916.t1 AT1G66270 43.844 463 250 7 22 480 57 513 2.81e-129 385
MS.gene003916.t1 AT5G26000 47.277 404 193 6 29 422 63 456 5.11e-127 377
MS.gene003916.t1 AT4G21760 42.670 457 241 7 21 475 74 511 6.03e-127 380
MS.gene003916.t1 AT1G61810 44.045 445 243 5 21 462 53 494 1.46e-126 379
MS.gene003916.t1 AT4G22100 43.290 462 236 9 21 476 42 483 1.54e-126 378
MS.gene003916.t1 AT1G60090 42.516 461 239 9 21 476 44 483 1.65e-126 378
MS.gene003916.t1 AT2G44480 57.241 290 122 2 22 311 87 374 1.65e-125 371
MS.gene003916.t1 AT4G27820 42.117 463 245 7 21 479 43 486 8.97e-124 371
MS.gene003916.t1 AT1G60090 41.810 464 245 10 21 476 44 490 8.08e-123 369
MS.gene003916.t1 AT1G45191 41.126 462 250 8 16 476 44 484 1.39e-119 360
MS.gene003916.t1 AT1G45191 40.645 465 254 8 16 476 44 490 1.38e-117 355
MS.gene003916.t1 AT3G62750 41.126 462 240 9 21 478 43 476 1.88e-117 354
MS.gene003916.t1 AT2G44470 48.830 342 167 4 10 345 39 378 2.47e-117 350
MS.gene003916.t1 AT1G45191 40.127 471 253 8 16 476 44 495 3.35e-117 354
MS.gene003916.t1 AT3G62750 41.037 463 241 9 21 479 69 503 4.28e-117 354
MS.gene003916.t1 AT5G48375 41.403 442 201 6 29 468 54 439 8.03e-116 348
MS.gene003916.t1 AT1G61810 42.995 414 230 5 72 482 4 414 1.10e-115 347
MS.gene003916.t1 AT1G45191 39.540 478 258 9 16 476 44 507 1.76e-114 348
MS.gene003916.t1 AT2G44460 49.454 366 179 5 120 481 1 364 2.26e-114 344
MS.gene003916.t1 AT4G27830 41.928 415 222 7 69 479 1 400 1.34e-112 339
MS.gene003916.t1 AT1G02850 45.038 393 173 6 22 386 47 424 7.44e-110 333
MS.gene003916.t1 AT3G62740 37.917 480 257 10 21 484 43 497 8.24e-110 335
MS.gene003916.t1 AT1G52400 44.581 406 214 8 22 421 60 460 2.00e-109 332
MS.gene003916.t1 AT1G61820 45.283 371 198 4 112 479 1 369 3.85e-106 321
MS.gene003916.t1 AT4G27820 40.964 415 227 6 69 479 1 401 3.30e-104 318
MS.gene003916.t1 AT4G27820 40.964 415 227 6 69 479 1 401 3.30e-104 318
MS.gene003916.t1 AT1G61810 42.818 369 206 4 21 386 53 419 4.60e-102 312
MS.gene003916.t1 AT2G44480 48.173 301 155 1 178 478 6 305 7.47e-101 305
MS.gene003916.t1 AT3G62750 41.032 407 209 8 21 424 69 447 1.08e-99 307
MS.gene003916.t1 AT3G18070 46.037 328 168 6 153 477 100 421 2.39e-99 305
MS.gene003916.t1 AT3G18070 48.161 299 149 4 22 320 51 343 2.61e-99 304
MS.gene003916.t1 AT3G62750 39.759 415 223 8 69 479 1 392 1.12e-98 303
MS.gene003916.t1 AT3G62750 39.759 415 223 8 69 479 1 392 1.12e-98 303
MS.gene003916.t1 AT1G60270 48.805 293 143 3 21 313 44 329 2.11e-98 301
MS.gene003916.t1 AT1G51490 46.356 343 163 8 13 349 30 357 1.91e-96 297
MS.gene003916.t1 AT3G62740 38.670 406 219 8 21 421 43 423 7.00e-94 291
MS.gene003916.t1 AT3G18070 45.714 315 162 6 166 477 1 309 9.40e-94 287
MS.gene003916.t1 AT3G62750 44.554 303 150 5 21 322 43 328 5.99e-91 282
MS.gene003916.t1 AT3G62750 44.554 303 150 5 21 322 43 328 5.99e-91 282
MS.gene003916.t1 AT1G45191 44.262 305 162 3 16 320 44 340 8.35e-91 283
MS.gene003916.t1 AT1G45191 44.262 305 162 3 16 320 44 340 1.56e-90 282
MS.gene003916.t1 AT3G62750 44.554 303 150 5 21 322 69 354 3.23e-90 282
MS.gene003916.t1 AT1G61810 48.400 250 127 2 21 270 53 300 2.58e-80 252
MS.gene003916.t1 AT1G60270 48.971 243 121 2 71 313 3 242 9.92e-80 250
MS.gene003916.t1 AT3G18070 44.068 236 128 3 153 386 100 333 2.85e-66 217
MS.gene003916.t1 AT3G18070 43.498 223 122 3 166 386 1 221 8.21e-61 199
MS.gene003916.t1 AT5G16580 37.631 287 164 7 147 432 27 299 8.06e-51 175
MS.gene003916.t1 AT3G06510 26.014 419 237 13 64 476 159 510 6.08e-31 126
MS.gene003916.t1 AT3G06510 25.799 438 248 13 64 476 159 544 1.82e-30 125

Find 115 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCTCAATATGGTATAAT+AGG 0.209400 4.2:+39443263 MS.gene003916:CDS
ATGGAAAAGGGCCAAGTATT+TGG 0.219721 4.2:+39441494 MS.gene003916:CDS
GACCTTTGCTACAAGGAATT+TGG 0.235205 4.2:+39442808 MS.gene003916:CDS
AAAACAGAAGACCTGATATT+TGG 0.235314 4.2:-39443031 None:intergenic
GTAATTTACATTACCGAAAA+TGG 0.241625 4.2:+39444196 MS.gene003916:CDS
CGATTCTGTAAGTATCTAAA+AGG 0.249841 4.2:-39444357 None:intergenic
TTCGGGAACAGAACCATATT+TGG 0.289048 4.2:+39442957 MS.gene003916:CDS
CAACCTCATTGACGAAATAT+TGG 0.306431 4.2:+39442459 MS.gene003916:CDS
AATCATAAAGGATTTGAATA+TGG 0.307459 4.2:+39442260 MS.gene003916:CDS
AAGATCCTCGGATTCTTCTT+TGG 0.311879 4.2:-39443728 None:intergenic
AACCAGTGTGCCGAGAGTTT+TGG 0.323939 4.2:-39444685 None:intergenic
TGGTAGTGTGCAGCCAAATA+TGG 0.343520 4.2:-39442970 None:intergenic
CGAGGCCTCGACTTCATGTT+TGG 0.348528 4.2:+39443353 MS.gene003916:CDS
GATTCTTCAAGCGACAGTTT+TGG 0.358175 4.2:-39444334 None:intergenic
AGAAGAATCCGAGGATCTTA+AGG 0.358352 4.2:+39443732 MS.gene003916:CDS
GGACGTTCCTCAAGCCTTAG+AGG 0.360927 4.2:+39442598 MS.gene003916:CDS
TGCATTTATCCGCGAGGATT+AGG 0.361716 4.2:+39444133 MS.gene003916:CDS
TGGAAAAGGGCCAAGTATTT+GGG 0.364918 4.2:+39441495 MS.gene003916:CDS
TATGTATTTCAGGAAGATAT+TGG 0.369128 4.2:+39442237 MS.gene003916:intron
GCTACAAGGAATTTGGTGAT+AGG 0.371977 4.2:+39442815 MS.gene003916:CDS
TAAAGCCAGATTCCCATTCA+TGG 0.377760 4.2:-39444609 None:intergenic
AAAATAAGAGATGGAGGTAA+TGG 0.386283 4.2:+39442112 MS.gene003916:CDS
GGAAGATATTGGAATCATAA+AGG 0.386661 4.2:+39442248 MS.gene003916:intron
AATTGCACAGGAGGTGATTC+GGG 0.389673 4.2:+39442940 MS.gene003916:CDS
ATCAGTTTGTATGGCGGGTC+TGG 0.391534 4.2:-39443827 None:intergenic
GATAAGAGAATCAGTTTGTA+TGG 0.395420 4.2:-39443836 None:intergenic
TCTGGCTTTAGCTTGCGATT+TGG 0.404401 4.2:+39444622 MS.gene003916:CDS
GATAACCATGAATGGGAATC+TGG 0.405714 4.2:+39444604 MS.gene003916:CDS
GAATTGCACAGGAGGTGATT+CGG 0.408321 4.2:+39442939 MS.gene003916:CDS
TATATGCAGGCATCTCAATA+TGG 0.410448 4.2:+39443254 MS.gene003916:intron
CATCTCAATATGGTATAATA+GGG 0.411825 4.2:+39443264 MS.gene003916:CDS
AGGTGCATCAGCAGCATAAG+AGG 0.412498 4.2:-39443791 None:intergenic
TATAGTAGTTTATTCCTTCA+CGG 0.415321 4.2:-39442435 None:intergenic
AGGGAAGGTGTGAATGTAAA+GGG 0.435489 4.2:+39444559 MS.gene003916:intron
ATTATGTTCAAGCTGCAATC+AGG 0.440754 4.2:+39444401 MS.gene003916:CDS
GATCGGAAAAGTCGCGAAGA+TGG 0.446253 4.2:-39441258 None:intergenic
TTTGCAGAAGGAAAGCTTAG+TGG 0.446993 4.2:+39442403 MS.gene003916:intron
ACTATACAAGACCAAATATC+AGG 0.449280 4.2:+39443020 MS.gene003916:CDS
GGATTACCTTACTCTCTCAT+TGG 0.457583 4.2:+39443285 MS.gene003916:CDS
CTTTGGAGAACTTTGGTAGC+CGG 0.461441 4.2:-39443711 None:intergenic
GAAGAATCCGAGGATCTTAA+GGG 0.463005 4.2:+39443733 MS.gene003916:CDS
AAGAGGATGAGTAATAGTTT+AGG 0.463613 4.2:-39443774 None:intergenic
CACCGGGTCGATGTTCAGAT+TGG 0.463655 4.2:+39442908 MS.gene003916:CDS
GCGATTTGGACTCGTATTTG+TGG 0.465681 4.2:+39444636 MS.gene003916:CDS
CTACAAGGAATTTGGTGATA+GGG 0.466831 4.2:+39442816 MS.gene003916:CDS
ACCGTCATACTCATCCTCTA+AGG 0.470920 4.2:-39442612 None:intergenic
GATTCTTCTTTGGAGAACTT+TGG 0.475194 4.2:-39443718 None:intergenic
TGCAATCTTTGGTTGGAAGC+CGG 0.480313 4.2:+39443692 MS.gene003916:CDS
AGCATGAATGCTTATGCATA+TGG 0.485187 4.2:+39442877 MS.gene003916:CDS
CATCGTGATGGCAAGCCCCT+TGG 0.493086 4.2:+39443964 MS.gene003916:CDS
GCAGCATTTGAAGATGGAAA+AGG 0.495549 4.2:+39441481 MS.gene003916:CDS
CAGGGAAGGTGTGAATGTAA+AGG 0.498317 4.2:+39444558 MS.gene003916:intron
CTCATACCAATGAGAGAGTA+AGG 0.499326 4.2:-39443291 None:intergenic
TCTGCTTTCCTTTGAGAGGC+CGG 0.500049 4.2:-39443314 None:intergenic
ATTGACGAAATATTGGCAAA+TGG 0.504849 4.2:+39442466 MS.gene003916:CDS
AGAGAATCAGTTTGTATGGC+GGG 0.508377 4.2:-39443832 None:intergenic
CTATGCTGACCTTTGCTACA+AGG 0.508555 4.2:+39442801 MS.gene003916:CDS
CAGCAGCATGAGCAAGAAGT+TGG 0.509755 4.2:-39442990 None:intergenic
TATATGGATCCAATCACAAA+AGG 0.510867 4.2:+39443652 MS.gene003916:CDS
CATTGTTGGATAACCATGAA+TGG 0.514197 4.2:+39444596 MS.gene003916:CDS
CACCAAATTCCTTGTAGCAA+AGG 0.520911 4.2:-39442810 None:intergenic
CAATGGAAAAGTGTCACATA+TGG 0.522829 4.2:-39442575 None:intergenic
ATTGTTGGATAACCATGAAT+GGG 0.522990 4.2:+39444597 MS.gene003916:CDS
AATATCTGCTTTCCTTTGAG+AGG 0.527824 4.2:-39443318 None:intergenic
TGGTTAAATCTGAATTGCAC+AGG 0.528273 4.2:+39442928 MS.gene003916:CDS
TTGGTGATAGGGTGAAACAT+TGG 0.532663 4.2:+39442827 MS.gene003916:CDS
ACTCTCTCATTGGTATGAGC+CGG 0.532738 4.2:+39443295 MS.gene003916:CDS
TGGCAAGCCCCTTGGTCCAA+TGG 0.533454 4.2:+39443972 MS.gene003916:CDS
TGCATATGGAAAGTTTGCAC+CGG 0.533544 4.2:+39442891 MS.gene003916:CDS
CAGCATTTGAAGATGGAAAA+GGG 0.539155 4.2:+39441482 MS.gene003916:CDS
GATTCTGTAAGTATCTAAAA+GGG 0.541771 4.2:-39444356 None:intergenic
AAAAGCATGCAATCTTTGGT+TGG 0.542865 4.2:+39443685 MS.gene003916:CDS
AATATATACCATTGGACCAA+GGG 0.544956 4.2:-39443980 None:intergenic
TCGATGACAGTTCATCGTGA+TGG 0.548253 4.2:+39443952 MS.gene003916:intron
GCCTCGACTTCATGTTTGGA+TGG 0.549073 4.2:+39443357 MS.gene003916:CDS
GAAGGTGCAGCATTTGAAGA+TGG 0.552430 4.2:+39441475 MS.gene003916:CDS
GTGAAAGTATCCCAAATACT+TGG 0.559221 4.2:-39441505 None:intergenic
TTGCCAATATTTCGTCAATG+AGG 0.564273 4.2:-39442462 None:intergenic
ATATGCAGTCTGCTTCAAGT+TGG 0.568943 4.2:+39444107 MS.gene003916:intron
CGAAGATGGAGTAGTGAAGA+AGG 0.570282 4.2:-39441244 None:intergenic
CGACGCATATCATCGGTACA+AGG 0.572001 4.2:+39442147 MS.gene003916:CDS
GCATTCATGCTCACACTCCA+TGG 0.576199 4.2:-39442865 None:intergenic
GAAACAGGTCCGATTGAGAT+CGG 0.577440 4.2:-39441275 None:intergenic
GAAAAGACACCCAAAACTCT+CGG 0.586397 4.2:+39444675 MS.gene003916:CDS
GCATATGGAAAGTTTGCACC+GGG 0.592559 4.2:+39442892 MS.gene003916:CDS
TACTCATCCTCTAAGGCTTG+AGG 0.594134 4.2:-39442605 None:intergenic
TAGCCGACGACGCATATCAT+CGG 0.598194 4.2:+39442140 MS.gene003916:CDS
AAATATATACCATTGGACCA+AGG 0.598574 4.2:-39443981 None:intergenic
GGTATGAGCCGGCCTCTCAA+AGG 0.599811 4.2:+39443306 MS.gene003916:CDS
CTTGAACATAATAAAGATGA+CGG 0.602302 4.2:-39444390 None:intergenic
ACCCAAAACTCTCGGCACAC+TGG 0.603547 4.2:+39444683 MS.gene003916:CDS
GGATTACTTTGAATGAACCA+TGG 0.603853 4.2:+39442848 MS.gene003916:CDS
AGGCTTGAGGAACGTCCCAA+TGG 0.604842 4.2:-39442592 None:intergenic
GTTTAGGCCAAGAAAATCGA+AGG 0.606609 4.2:-39443758 None:intergenic
GAAGGACCCCTTAAGATCCT+CGG 0.611639 4.2:-39443740 None:intergenic
AACCAATCTGAACATCGACC+CGG 0.611784 4.2:-39442910 None:intergenic
GGTGTCACTCACACAATCCG+AGG 0.616673 4.2:-39442644 None:intergenic
GGTCTGGCATCGCGTAAGTG+AGG 0.627761 4.2:-39443811 None:intergenic
GCCTTAGAGGATGAGTATGA+CGG 0.628770 4.2:+39442611 MS.gene003916:CDS
GCAGAAGGAAAGCTTAGTGG+AGG 0.637778 4.2:+39442406 MS.gene003916:intron
ATATATACCATTGGACCAAG+GGG 0.639799 4.2:-39443979 None:intergenic
AGTGGAGGTGTAAACCGTGA+AGG 0.641058 4.2:+39442421 MS.gene003916:CDS
ACCATCCAAACATGAAGTCG+AGG 0.641696 4.2:-39443358 None:intergenic
GTTCTCCAAAGAAGAATCCG+AGG 0.647399 4.2:+39443723 MS.gene003916:CDS
AACAGTTGTCCTAATCCTCG+CGG 0.657090 4.2:-39444142 None:intergenic
AAGAGAATCAGTTTGTATGG+CGG 0.658985 4.2:-39443833 None:intergenic
TAGCACTCTAGACCAAGCAA+TGG 0.660595 4.2:-39442298 None:intergenic
GTGTCACTCACACAATCCGA+GGG 0.662250 4.2:-39442643 None:intergenic
TTAAATCTGAATTGCACAGG+AGG 0.663041 4.2:+39442931 MS.gene003916:CDS
GTACCGATGATATGCGTCGT+CGG 0.669538 4.2:-39442143 None:intergenic
TTTCGGTAATGTAAATTACG+GGG 0.671497 4.2:-39444192 None:intergenic
AAGAATCCGAGGATCTTAAG+GGG 0.674534 4.2:+39443734 MS.gene003916:CDS
ACTTTCACTCATGAACACCC+AGG 0.690909 4.2:+39441520 MS.gene003916:CDS
GATATTGATGCTGCACAACG+AGG 0.693108 4.2:+39443335 MS.gene003916:CDS
TGGTTATGCATTTATCCGCG+AGG 0.715115 4.2:+39444127 MS.gene003916:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATTTATGACTATTTA+GGG + chr4.2:39442684-39442703 MS.gene003916:intron 10.0%
!!! TACAAATTTTTTTTTTGAAA+AGG - chr4.2:39441873-39441892 None:intergenic 10.0%
!! AATGATAAAATATATACCAT+TGG - chr4.2:39443991-39444010 None:intergenic 15.0%
!! ACTGCATATAATTAAAAAAA+AGG - chr4.2:39444096-39444115 None:intergenic 15.0%
!! AGCAATATATTATTCAAATT+CGG + chr4.2:39443058-39443077 MS.gene003916:intron 15.0%
!! AGTTTATAAGAAATTTATGA+TGG - chr4.2:39442382-39442401 None:intergenic 15.0%
!! CTATTTATTTATGACTATTT+AGG + chr4.2:39442683-39442702 MS.gene003916:intron 15.0%
!! TCTCATATATAATAATGAAA+TGG + chr4.2:39442080-39442099 MS.gene003916:intron 15.0%
!!! AAATTTTGAAATATATATGC+AGG + chr4.2:39443241-39443260 MS.gene003916:intron 15.0%
!!! ATTTCAAAATTTTGGTTATT+TGG - chr4.2:39443233-39443252 None:intergenic 15.0%
!!! GCATATATATTTCAAAATTT+TGG - chr4.2:39443241-39443260 None:intergenic 15.0%
!!! TAATATCATGTTTCTTTTTT+TGG + chr4.2:39441587-39441606 MS.gene003916:intron 15.0%
!!! TTAAATTTTTTGTAGTATGA+AGG + chr4.2:39441457-39441476 MS.gene003916:intron 15.0%
!! AATCATAAAGGATTTGAATA+TGG + chr4.2:39442260-39442279 MS.gene003916:CDS 20.0%
!! AGAACTAATTAAAAATAACC+TGG - chr4.2:39441541-39441560 None:intergenic 20.0%
!! ATAGGTAAATGTAAAAATGA+AGG - chr4.2:39444519-39444538 None:intergenic 20.0%
!! GAACTAATTAAAAATAACCT+GGG - chr4.2:39441540-39441559 None:intergenic 20.0%
!! TAGGTAAATGTAAAAATGAA+GGG - chr4.2:39444518-39444537 None:intergenic 20.0%
!! TATTCATAAAGGTCAATAAT+AGG - chr4.2:39444537-39444556 None:intergenic 20.0%
!!! AATAGAGTTATTACCATTTT+CGG - chr4.2:39444212-39444231 None:intergenic 20.0%
!!! ATGTAAACTTCATTTGATTT+TGG + chr4.2:39443477-39443496 MS.gene003916:intron 20.0%
!!! ATTTTCGGTAATGTAAATTA+CGG - chr4.2:39444197-39444216 None:intergenic 20.0%
!!! GTTTCTTTTTTTGGTAATAT+GGG + chr4.2:39441596-39441615 MS.gene003916:intron 20.0%
!!! TGTTTCTTTTTTTGGTAATA+TGG + chr4.2:39441595-39441614 MS.gene003916:intron 20.0%
! AAATGTTGCACTAAATTGAA+TGG - chr4.2:39441849-39441868 None:intergenic 25.0%
! AAGTGACAAAATCAATTGAT+AGG + chr4.2:39444292-39444311 MS.gene003916:intron 25.0%
! AATAGCTCAAGATTGAAAAT+TGG - chr4.2:39442668-39442687 None:intergenic 25.0%
! AGTAGCTAAAACTTAATTAG+AGG - chr4.2:39444450-39444469 None:intergenic 25.0%
! ATATTATTTGGACATGTTGT+AGG - chr4.2:39441628-39441647 None:intergenic 25.0%
! ATTGACCTTTATGAATAACA+GGG + chr4.2:39444540-39444559 MS.gene003916:intron 25.0%
! CATAAATCACCAATGTATAA+GGG - chr4.2:39442355-39442374 None:intergenic 25.0%
! CATCTCAATATGGTATAATA+GGG + chr4.2:39443264-39443283 MS.gene003916:CDS 25.0%
! CTTGAACATAATAAAGATGA+CGG - chr4.2:39444393-39444412 None:intergenic 25.0%
! GAAACTCTATCTCAATATAA+CGG + chr4.2:39441712-39441731 MS.gene003916:intron 25.0%
! GATTCTGTAAGTATCTAAAA+GGG - chr4.2:39444359-39444378 None:intergenic 25.0%
! GTAATTTACATTACCGAAAA+TGG + chr4.2:39444196-39444215 MS.gene003916:CDS 25.0%
! TAAATCGTTACATTAAGAGA+GGG + chr4.2:39441690-39441709 MS.gene003916:intron 25.0%
! TAGATTACTCTATGTATTTC+AGG + chr4.2:39442227-39442246 MS.gene003916:intron 25.0%
! TATAGTAGTTTATTCCTTCA+CGG - chr4.2:39442438-39442457 None:intergenic 25.0%
! TATGTATTTCAGGAAGATAT+TGG + chr4.2:39442237-39442256 MS.gene003916:intron 25.0%
! TATTGACCTTTATGAATAAC+AGG + chr4.2:39444539-39444558 MS.gene003916:intron 25.0%
! TCTTATAAACTATTTGCAGA+AGG + chr4.2:39442391-39442410 MS.gene003916:intron 25.0%
! TTACAAAACTATGACTTACT+TGG - chr4.2:39442324-39442343 None:intergenic 25.0%
!! CTTTTCCTTAATGATGTATT+TGG + chr4.2:39441926-39441945 MS.gene003916:intron 25.0%
!! TCTCATGTTATTTGAATTAC+AGG - chr4.2:39443100-39443119 None:intergenic 25.0%
!! TTTTATGATTAACAACCACA+TGG - chr4.2:39442526-39442545 None:intergenic 25.0%
!! TTTTCGGTAATGTAAATTAC+GGG - chr4.2:39444196-39444215 None:intergenic 25.0%
!!! AGTTTTAGCTACTGATTTAT+AGG + chr4.2:39444457-39444476 MS.gene003916:intron 25.0%
!!! CATTTTGCATTTTTTTCTGT+AGG + chr4.2:39443629-39443648 MS.gene003916:intron 25.0%
!!! CATTTTTTTCTGTAGGTATA+TGG + chr4.2:39443636-39443655 MS.gene003916:intron 25.0%
AAAACAGAAGACCTGATATT+TGG - chr4.2:39443034-39443053 None:intergenic 30.0%
AAAATAAGAGATGGAGGTAA+TGG + chr4.2:39442112-39442131 MS.gene003916:CDS 30.0%
AAATATATACCATTGGACCA+AGG - chr4.2:39443984-39444003 None:intergenic 30.0%
AAGAGGATGAGTAATAGTTT+AGG - chr4.2:39443777-39443796 None:intergenic 30.0%
AATATATACCATTGGACCAA+GGG - chr4.2:39443983-39444002 None:intergenic 30.0%
AATATTCCTCCCTTATACAT+TGG + chr4.2:39442343-39442362 MS.gene003916:intron 30.0%
AATTCATGCCTGTTCATTTA+GGG + chr4.2:39441766-39441785 MS.gene003916:intron 30.0%
ACTATACAAGACCAAATATC+AGG + chr4.2:39443020-39443039 MS.gene003916:CDS 30.0%
AGCAAAAACTTATGCTATGT+TGG - chr4.2:39442012-39442031 None:intergenic 30.0%
ATAATCATTGAGAACAATGC+AGG + chr4.2:39442545-39442564 MS.gene003916:intron 30.0%
ATCTGATCTTGTGTAATAGA+AGG + chr4.2:39443119-39443138 MS.gene003916:intron 30.0%
ATTGACGAAATATTGGCAAA+TGG + chr4.2:39442466-39442485 MS.gene003916:CDS 30.0%
ATTGTTGGATAACCATGAAT+GGG + chr4.2:39444597-39444616 MS.gene003916:CDS 30.0%
CCATAAATCACCAATGTATA+AGG - chr4.2:39442356-39442375 None:intergenic 30.0%
CGATTCTGTAAGTATCTAAA+AGG - chr4.2:39444360-39444379 None:intergenic 30.0%
CTTGCAGAAAAAATAAGAGA+TGG + chr4.2:39442103-39442122 MS.gene003916:intron 30.0%
GATAAGAGAATCAGTTTGTA+TGG - chr4.2:39443839-39443858 None:intergenic 30.0%
GCATCTCAATATGGTATAAT+AGG + chr4.2:39443263-39443282 MS.gene003916:CDS 30.0%
GGAAGATATTGGAATCATAA+AGG + chr4.2:39442248-39442267 MS.gene003916:intron 30.0%
GTAAATCGTTACATTAAGAG+AGG + chr4.2:39441689-39441708 MS.gene003916:intron 30.0%
TATATGGATCCAATCACAAA+AGG + chr4.2:39443652-39443671 MS.gene003916:CDS 30.0%
TCTGATCTTGTGTAATAGAA+GGG + chr4.2:39443120-39443139 MS.gene003916:intron 30.0%
TTAAACTTAATGCAACTTGC+TGG + chr4.2:39441903-39441922 MS.gene003916:intron 30.0%
TTGCACCAAATACATCATTA+AGG - chr4.2:39441934-39441953 None:intergenic 30.0%
TTTCGGTAATGTAAATTACG+GGG - chr4.2:39444195-39444214 None:intergenic 30.0%
! ATATGTGACACTTTTCCATT+GGG + chr4.2:39442577-39442596 MS.gene003916:CDS 30.0%
! ATATTTTGCATGGTCATTGT+TGG + chr4.2:39444582-39444601 MS.gene003916:CDS 30.0%
! ATCTTGCAGAGATGATTTTA+GGG + chr4.2:39442777-39442796 MS.gene003916:intron 30.0%
! ATGTAAAGGGATATTTTGCA+TGG + chr4.2:39444572-39444591 MS.gene003916:CDS 30.0%
! CCTTATACATTGGTGATTTA+TGG + chr4.2:39442353-39442372 MS.gene003916:intron 30.0%
! TCATTTAGGGCATAAATGAA+CGG + chr4.2:39441779-39441798 MS.gene003916:intron 30.0%
! TTTGTGTTTTCATATCCATG+TGG + chr4.2:39442508-39442527 MS.gene003916:intron 30.0%
!!! TTTCTTGGCAAGATTTTTTG+AGG - chr4.2:39444720-39444739 None:intergenic 30.0%
AAATCACCAATGTATAAGGG+AGG - chr4.2:39442352-39442371 None:intergenic 35.0%
AAGAGAATCAGTTTGTATGG+CGG - chr4.2:39443836-39443855 None:intergenic 35.0%
AATATCTGCTTTCCTTTGAG+AGG - chr4.2:39443321-39443340 None:intergenic 35.0%
ACACAAAAGCTACAAATGAG+AGG - chr4.2:39444041-39444060 None:intergenic 35.0%
ACCTTCCCTGTTATTCATAA+AGG - chr4.2:39444548-39444567 None:intergenic 35.0%
ACCTTTATGAATAACAGGGA+AGG + chr4.2:39444544-39444563 MS.gene003916:intron 35.0%
AGCATGAATGCTTATGCATA+TGG + chr4.2:39442877-39442896 MS.gene003916:CDS 35.0%
ATATATACCATTGGACCAAG+GGG - chr4.2:39443982-39444001 None:intergenic 35.0%
ATTATGTTCAAGCTGCAATC+AGG + chr4.2:39444401-39444420 MS.gene003916:CDS 35.0%
CAACCTCATTGACGAAATAT+TGG + chr4.2:39442459-39442478 MS.gene003916:CDS 35.0%
CAGCATTTGAAGATGGAAAA+GGG + chr4.2:39441482-39441501 MS.gene003916:CDS 35.0%
CATTGTTGGATAACCATGAA+TGG + chr4.2:39444596-39444615 MS.gene003916:CDS 35.0%
CATTTATGCCCTAAATGAAC+AGG - chr4.2:39441777-39441796 None:intergenic 35.0%
CCATTTGTGCAAAGTAACAT+AGG + chr4.2:39443567-39443586 MS.gene003916:intron 35.0%
GAATTCATGCCTGTTCATTT+AGG + chr4.2:39441765-39441784 MS.gene003916:intron 35.0%
GCAGAAAAAATAAGAGATGG+AGG + chr4.2:39442106-39442125 MS.gene003916:intron 35.0%
GGATTACTTTGAATGAACCA+TGG + chr4.2:39442848-39442867 MS.gene003916:CDS 35.0%
TATATGCAGGCATCTCAATA+TGG + chr4.2:39443254-39443273 MS.gene003916:intron 35.0%
TCCATTCATGCTATGTAACT+AGG - chr4.2:39442058-39442077 None:intergenic 35.0%
TGGTTAAATCTGAATTGCAC+AGG + chr4.2:39442928-39442947 MS.gene003916:CDS 35.0%
TGTGTAATAGAAGGGAAAGT+CGG + chr4.2:39443128-39443147 MS.gene003916:intron 35.0%
TTAAATCTGAATTGCACAGG+AGG + chr4.2:39442931-39442950 MS.gene003916:CDS 35.0%
TTGCCAATATTTCGTCAATG+AGG - chr4.2:39442465-39442484 None:intergenic 35.0%
! AAAAGCATGCAATCTTTGGT+TGG + chr4.2:39443685-39443704 MS.gene003916:CDS 35.0%
! ACCAAAGATTGCATGCTTTT+TGG - chr4.2:39443685-39443704 None:intergenic 35.0%
! CATATGTGACACTTTTCCAT+TGG + chr4.2:39442576-39442595 MS.gene003916:CDS 35.0%
! CTACAAGGAATTTGGTGATA+GGG + chr4.2:39442816-39442835 MS.gene003916:CDS 35.0%
! CTTACAGATTTTCCATTGCT+TGG + chr4.2:39442286-39442305 MS.gene003916:CDS 35.0%
! GATCTTGCAGAGATGATTTT+AGG + chr4.2:39442776-39442795 MS.gene003916:intron 35.0%
! GATTCTTCTTTGGAGAACTT+TGG - chr4.2:39443721-39443740 None:intergenic 35.0%
! GGATAGTTTCCTTTTGTGAT+TGG - chr4.2:39443664-39443683 None:intergenic 35.0%
! TCCAAAAAGCATGCAATCTT+TGG + chr4.2:39443681-39443700 MS.gene003916:CDS 35.0%
!! CAATGGAAAAGTGTCACATA+TGG - chr4.2:39442578-39442597 None:intergenic 35.0%
!! CCTATGTTACTTTGCACAAA+TGG - chr4.2:39443570-39443589 None:intergenic 35.0%
!! GTGAAAGTATCCCAAATACT+TGG - chr4.2:39441508-39441527 None:intergenic 35.0%
AAATCATCTCTGCAAGATCG+CGG - chr4.2:39442775-39442794 None:intergenic 40.0%
AACAGCTTCTTCTGCATTTC+AGG + chr4.2:39441318-39441337 MS.gene003916:intron 40.0%
AAGAATCCGAGGATCTTAAG+GGG + chr4.2:39443734-39443753 MS.gene003916:CDS 40.0%
AAGATCCTCGGATTCTTCTT+TGG - chr4.2:39443731-39443750 None:intergenic 40.0%
AGAAGAATCCGAGGATCTTA+AGG + chr4.2:39443732-39443751 MS.gene003916:CDS 40.0%
AGAGAATCAGTTTGTATGGC+GGG - chr4.2:39443835-39443854 None:intergenic 40.0%
AGCTCCTTTCTTTATGCTTG+TGG + chr4.2:39441810-39441829 MS.gene003916:intron 40.0%
ATATGCAGTCTGCTTCAAGT+TGG + chr4.2:39444107-39444126 MS.gene003916:intron 40.0%
ATGGAAAAGGGCCAAGTATT+TGG + chr4.2:39441494-39441513 MS.gene003916:CDS 40.0%
CACCAAATTCCTTGTAGCAA+AGG - chr4.2:39442813-39442832 None:intergenic 40.0%
CTCATACCAATGAGAGAGTA+AGG - chr4.2:39443294-39443313 None:intergenic 40.0%
GAAAAGACACCCAAAACTCT+CGG + chr4.2:39444675-39444694 MS.gene003916:CDS 40.0%
GAAGAATCCGAGGATCTTAA+GGG + chr4.2:39443733-39443752 MS.gene003916:CDS 40.0%
GACCTTTGCTACAAGGAATT+TGG + chr4.2:39442808-39442827 MS.gene003916:CDS 40.0%
GATAACCATGAATGGGAATC+TGG + chr4.2:39444604-39444623 MS.gene003916:CDS 40.0%
GATCCCACAAGCATAAAGAA+AGG - chr4.2:39441817-39441836 None:intergenic 40.0%
GCAGCATTTGAAGATGGAAA+AGG + chr4.2:39441481-39441500 MS.gene003916:CDS 40.0%
GCCTAGTTACATAGCATGAA+TGG + chr4.2:39442054-39442073 MS.gene003916:intron 40.0%
GCTCCTTTCTTTATGCTTGT+GGG + chr4.2:39441811-39441830 MS.gene003916:intron 40.0%
GGATTACCTTACTCTCTCAT+TGG + chr4.2:39443285-39443304 MS.gene003916:CDS 40.0%
GTTTAGGCCAAGAAAATCGA+AGG - chr4.2:39443761-39443780 None:intergenic 40.0%
TAAAGCCAGATTCCCATTCA+TGG - chr4.2:39444612-39444631 None:intergenic 40.0%
TAAGTAGCTTCTGAATGCCT+TGG + chr4.2:39441982-39442001 MS.gene003916:intron 40.0%
TATGCTATGTTGGATCTCCA+AGG - chr4.2:39442002-39442021 None:intergenic 40.0%
TGCATATGGAAAGTTTGCAC+CGG + chr4.2:39442891-39442910 MS.gene003916:CDS 40.0%
TGGAAAAGGGCCAAGTATTT+GGG + chr4.2:39441495-39441514 MS.gene003916:CDS 40.0%
TTCGGGAACAGAACCATATT+TGG + chr4.2:39442957-39442976 MS.gene003916:CDS 40.0%
TTTGCAGAAGGAAAGCTTAG+TGG + chr4.2:39442403-39442422 MS.gene003916:intron 40.0%
! AGGGAAGGTGTGAATGTAAA+GGG + chr4.2:39444559-39444578 MS.gene003916:intron 40.0%
! GATTCTTCAAGCGACAGTTT+TGG - chr4.2:39444337-39444356 None:intergenic 40.0%
! GCTACAAGGAATTTGGTGAT+AGG + chr4.2:39442815-39442834 MS.gene003916:CDS 40.0%
! TTGGTGATAGGGTGAAACAT+TGG + chr4.2:39442827-39442846 MS.gene003916:CDS 40.0%
!! CAAGGTATGTAGTTTTGCAG+AGG + chr4.2:39442165-39442184 MS.gene003916:intron 40.0%
!!! ATGACGGTTTTTTAAGCCCT+CGG + chr4.2:39442627-39442646 MS.gene003916:CDS 40.0%
AACAGTTGTCCTAATCCTCG+CGG - chr4.2:39444145-39444164 None:intergenic 45.0%
AACCAATCTGAACATCGACC+CGG - chr4.2:39442913-39442932 None:intergenic 45.0%
AATTGCACAGGAGGTGATTC+GGG + chr4.2:39442940-39442959 MS.gene003916:CDS 45.0%
ACCATCCAAACATGAAGTCG+AGG - chr4.2:39443361-39443380 None:intergenic 45.0%
ACCGTCATACTCATCCTCTA+AGG - chr4.2:39442615-39442634 None:intergenic 45.0%
ACTCTCTCATTGGTATGAGC+CGG + chr4.2:39443295-39443314 MS.gene003916:CDS 45.0%
ACTTTCACTCATGAACACCC+AGG + chr4.2:39441520-39441539 MS.gene003916:CDS 45.0%
AGGCATGAATTCGCTACAGT+TGG - chr4.2:39441757-39441776 None:intergenic 45.0%
CGAAGATGGAGTAGTGAAGA+AGG - chr4.2:39441247-39441266 None:intergenic 45.0%
CTATGCTGACCTTTGCTACA+AGG + chr4.2:39442801-39442820 MS.gene003916:CDS 45.0%
GAAACAGGTCCGATTGAGAT+CGG - chr4.2:39441278-39441297 None:intergenic 45.0%
GAATTGCACAGGAGGTGATT+CGG + chr4.2:39442939-39442958 MS.gene003916:CDS 45.0%
GATATTGATGCTGCACAACG+AGG + chr4.2:39443335-39443354 MS.gene003916:CDS 45.0%
GCATATGGAAAGTTTGCACC+GGG + chr4.2:39442892-39442911 MS.gene003916:CDS 45.0%
GCCTTAGAGGATGAGTATGA+CGG + chr4.2:39442611-39442630 MS.gene003916:CDS 45.0%
GGCATAAATGAACGGACATG+TGG + chr4.2:39441787-39441806 MS.gene003916:intron 45.0%
GTTCTCCAAAGAAGAATCCG+AGG + chr4.2:39443723-39443742 MS.gene003916:CDS 45.0%
TAGCACTCTAGACCAAGCAA+TGG - chr4.2:39442301-39442320 None:intergenic 45.0%
TCGATGACAGTTCATCGTGA+TGG + chr4.2:39443952-39443971 MS.gene003916:intron 45.0%
TGCAATCTTTGGTTGGAAGC+CGG + chr4.2:39443692-39443711 MS.gene003916:CDS 45.0%
TGCATTTATCCGCGAGGATT+AGG + chr4.2:39444133-39444152 MS.gene003916:CDS 45.0%
TGGTTATGCATTTATCCGCG+AGG + chr4.2:39444127-39444146 MS.gene003916:CDS 45.0%
! AAGGGGTCCTTCGATTTTCT+TGG + chr4.2:39443751-39443770 MS.gene003916:CDS 45.0%
! CAGGGAAGGTGTGAATGTAA+AGG + chr4.2:39444558-39444577 MS.gene003916:intron 45.0%
! CTTTGGAGAACTTTGGTAGC+CGG - chr4.2:39443714-39443733 None:intergenic 45.0%
! GAAGGTGCAGCATTTGAAGA+TGG + chr4.2:39441475-39441494 MS.gene003916:CDS 45.0%
! GCGACTTTTCCGATCTCAAT+CGG + chr4.2:39441266-39441285 MS.gene003916:CDS 45.0%
! GCGATTTGGACTCGTATTTG+TGG + chr4.2:39444636-39444655 MS.gene003916:CDS 45.0%
! TGGTAGTGTGCAGCCAAATA+TGG - chr4.2:39442973-39442992 None:intergenic 45.0%
!! GTTGGCTTTACGATGCAAGT+TGG - chr4.2:39441739-39441758 None:intergenic 45.0%
!! TACTCATCCTCTAAGGCTTG+AGG - chr4.2:39442608-39442627 None:intergenic 45.0%
!! TCTGGCTTTAGCTTGCGATT+TGG + chr4.2:39444622-39444641 MS.gene003916:CDS 45.0%
!! AATAACATATTAATATTATT+TGG - chr4.2:39441640-39441659 None:intergenic 5.0%
AGGTGCATCAGCAGCATAAG+AGG - chr4.2:39443794-39443813 None:intergenic 50.0%
CAGCAGCATGAGCAAGAAGT+TGG - chr4.2:39442993-39443012 None:intergenic 50.0%
CGACGCATATCATCGGTACA+AGG + chr4.2:39442147-39442166 MS.gene003916:CDS 50.0%
GAAGGACCCCTTAAGATCCT+CGG - chr4.2:39443743-39443762 None:intergenic 50.0%
GATCGGAAAAGTCGCGAAGA+TGG - chr4.2:39441261-39441280 None:intergenic 50.0%
GCAGAAGGAAAGCTTAGTGG+AGG + chr4.2:39442406-39442425 MS.gene003916:intron 50.0%
GCATTCATGCTCACACTCCA+TGG - chr4.2:39442868-39442887 None:intergenic 50.0%
GCCTCGACTTCATGTTTGGA+TGG + chr4.2:39443357-39443376 MS.gene003916:CDS 50.0%
GCTGTTCCGAAAACAAAGCC+TGG - chr4.2:39441304-39441323 None:intergenic 50.0%
GTTCCGAAAACAAAGCCTGG+CGG - chr4.2:39441301-39441320 None:intergenic 50.0%
TAGCCGACGACGCATATCAT+CGG + chr4.2:39442140-39442159 MS.gene003916:CDS 50.0%
TTCCGAAAACAAAGCCTGGC+GGG - chr4.2:39441300-39441319 None:intergenic 50.0%
! AACCAGTGTGCCGAGAGTTT+TGG - chr4.2:39444688-39444707 None:intergenic 50.0%
! ACCAGTGTGCCGAGAGTTTT+GGG - chr4.2:39444687-39444706 None:intergenic 50.0%
! AGTGGAGGTGTAAACCGTGA+AGG + chr4.2:39442421-39442440 MS.gene003916:CDS 50.0%
! GTACCGATGATATGCGTCGT+CGG - chr4.2:39442146-39442165 None:intergenic 50.0%
! GTGTCACTCACACAATCCGA+GGG - chr4.2:39442646-39442665 None:intergenic 50.0%
! TCTGCTTTCCTTTGAGAGGC+CGG - chr4.2:39443317-39443336 None:intergenic 50.0%
!! ATCAGTTTGTATGGCGGGTC+TGG - chr4.2:39443830-39443849 None:intergenic 50.0%
!!! TTCCCGCCAGGCTTTGTTTT+CGG + chr4.2:39441295-39441314 MS.gene003916:intron 50.0%
AACAAAGCCTGGCGGGAAAC+AGG - chr4.2:39441293-39441312 None:intergenic 55.0%
ACCCAAAACTCTCGGCACAC+TGG + chr4.2:39444683-39444702 MS.gene003916:CDS 55.0%
AGGCTTGAGGAACGTCCCAA+TGG - chr4.2:39442595-39442614 None:intergenic 55.0%
CACCGGGTCGATGTTCAGAT+TGG + chr4.2:39442908-39442927 MS.gene003916:CDS 55.0%
CGAGGCCTCGACTTCATGTT+TGG + chr4.2:39443353-39443372 MS.gene003916:CDS 55.0%
GGACGTTCCTCAAGCCTTAG+AGG + chr4.2:39442598-39442617 MS.gene003916:CDS 55.0%
!! GGTGTCACTCACACAATCCG+AGG - chr4.2:39442647-39442666 None:intergenic 55.0%
AATCGGACCTGTTTCCCGCC+AGG + chr4.2:39441283-39441302 MS.gene003916:intron 60.0%
CATCGTGATGGCAAGCCCCT+TGG + chr4.2:39443964-39443983 MS.gene003916:CDS 60.0%
GGTATGAGCCGGCCTCTCAA+AGG + chr4.2:39443306-39443325 MS.gene003916:CDS 60.0%
GGTCTGGCATCGCGTAAGTG+AGG - chr4.2:39443814-39443833 None:intergenic 60.0%
TGGCAAGCCCCTTGGTCCAA+TGG + chr4.2:39443972-39443991 MS.gene003916:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 39441233 39444753 39441233 ID=MS.gene003916
chr4.2 mRNA 39441233 39444753 39441233 ID=MS.gene003916.t1;Parent=MS.gene003916
chr4.2 exon 39441233 39441292 39441233 ID=MS.gene003916.t1.exon1;Parent=MS.gene003916.t1
chr4.2 CDS 39441233 39441292 39441233 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39441472 39441541 39441472 ID=MS.gene003916.t1.exon2;Parent=MS.gene003916.t1
chr4.2 CDS 39441472 39441541 39441472 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39442110 39442168 39442110 ID=MS.gene003916.t1.exon3;Parent=MS.gene003916.t1
chr4.2 CDS 39442110 39442168 39442110 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39442249 39442324 39442249 ID=MS.gene003916.t1.exon4;Parent=MS.gene003916.t1
chr4.2 CDS 39442249 39442324 39442249 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39442410 39442487 39442410 ID=MS.gene003916.t1.exon5;Parent=MS.gene003916.t1
chr4.2 CDS 39442410 39442487 39442410 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39442567 39442654 39442567 ID=MS.gene003916.t1.exon6;Parent=MS.gene003916.t1
chr4.2 CDS 39442567 39442654 39442567 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39442786 39443041 39442786 ID=MS.gene003916.t1.exon7;Parent=MS.gene003916.t1
chr4.2 CDS 39442786 39443041 39442786 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39443263 39443378 39443263 ID=MS.gene003916.t1.exon8;Parent=MS.gene003916.t1
chr4.2 CDS 39443263 39443378 39443263 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39443651 39443868 39443651 ID=MS.gene003916.t1.exon9;Parent=MS.gene003916.t1
chr4.2 CDS 39443651 39443868 39443651 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39443962 39443993 39443962 ID=MS.gene003916.t1.exon10;Parent=MS.gene003916.t1
chr4.2 CDS 39443962 39443993 39443962 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39444115 39444217 39444115 ID=MS.gene003916.t1.exon11;Parent=MS.gene003916.t1
chr4.2 CDS 39444115 39444217 39444115 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39444314 39444422 39444314 ID=MS.gene003916.t1.exon12;Parent=MS.gene003916.t1
chr4.2 CDS 39444314 39444422 39444314 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
chr4.2 exon 39444561 39444753 39444561 ID=MS.gene003916.t1.exon13;Parent=MS.gene003916.t1
chr4.2 CDS 39444561 39444753 39444561 ID=cds.MS.gene003916.t1;Parent=MS.gene003916.t1
Gene Sequence

>MS.gene003916

ATGCTTTTGAACCTTCTTCACTACTCCATCTTCGCGACTTTTCCGATCTCAATCGGACCTGTTTCCCGCCAGGCTTTGTTTTCGGAACAGCTTCTTCTGCATTTCAGGCATGCACAAACAAACATCTTTCAATTTTATTATTATTATTATTTATAACATGCATGCAATGTAGAAATGTAGTTATTAGAGATTTGATTTTGTGTAATGATTAAGTTTCATGTTATTTAAATTTTTTGTAGTATGAAGGTGCAGCATTTGAAGATGGAAAAGGGCCAAGTATTTGGGATACTTTCACTCATGAACACCCAGGTTATTTTTAATTAGTTCTCTTTCATTTTGCAATTTTGAAAATATTAATATCATGTTTCTTTTTTTGGTAATATGGGTTCAAACCTACAACATGTCCAAATAATATTAATATGTTATTTTACATCACGCTTTATTGCACATCTCATAGTAAATCGTTACATTAAGAGAGGGAAACTCTATCTCAATATAACGGTCCAACTTGCATCGTAAAGCCAACTGTAGCGAATTCATGCCTGTTCATTTAGGGCATAAATGAACGGACATGTGGAGCTCCTTTCTTTATGCTTGTGGGATCTTGCATGTTCCATTCAATTTAGTGCAACATTTGCCTTTTCAAAAAAAAAATTTGTAATAATAATAATTAAACTTAATGCAACTTGCTGGCTTTTCCTTAATGATGTATTTGGTGCAAGAATCATGATCAACAGTCATTTTCAGCATAAGTAGCTTCTGAATGCCTTGGAGATCCAACATAGCATAAGTTTTTGCTTGCTTTACATATATATTGAATTGCCTAGTTACATAGCATGAATGGATTTCTCATATATAATAATGAAATGGCTTGCAGAAAAAATAAGAGATGGAGGTAATGGAGATGTAGCCGACGACGCATATCATCGGTACAAGGTATGTAGTTTTGCAGAGGTATATAAAATACAAATGTAACATAGATTGAGACTCATGATAGATTACTCTATGTATTTCAGGAAGATATTGGAATCATAAAGGATTTGAATATGGATGCTTACAGATTTTCCATTGCTTGGTCTAGAGTGCTACCAAGTAAGTCATAGTTTTGTAAATATTCCTCCCTTATACATTGGTGATTTATGGTAACCATCATAAATTTCTTATAAACTATTTGCAGAAGGAAAGCTTAGTGGAGGTGTAAACCGTGAAGGAATAAACTACTATAACAACCTCATTGACGAAATATTGGCAAATGGTAAAATTCATCAAAATTTATTTGTGTTTTCATATCCATGTGGTTGTTAATCATAAAATAATCATTGAGAACAATGCAGGTTTGCAACCATATGTGACACTTTTCCATTGGGACGTTCCTCAAGCCTTAGAGGATGAGTATGACGGTTTTTTAAGCCCTCGGATTGTGTGAGTGACACCAATTTTCAATCTTGAGCTATTTATTTATGACTATTTAGGGAAATATTGCAGTAAATAAAATTAATCGATTCTTCTTCAAAAAAAAAAATTAATCGATTATGTAATCCGCGATCTTGCAGAGATGATTTTAGGGACTATGCTGACCTTTGCTACAAGGAATTTGGTGATAGGGTGAAACATTGGATTACTTTGAATGAACCATGGAGTGTGAGCATGAATGCTTATGCATATGGAAAGTTTGCACCGGGTCGATGTTCAGATTGGTTAAATCTGAATTGCACAGGAGGTGATTCGGGAACAGAACCATATTTGGCTGCACACTACCAACTTCTTGCTCATGCTGCTGCTACAAAACTATACAAGACCAAATATCAGGTCTTCTGTTTTTAAAAGCAATATATTATTCAAATTCGGCACTGAATTTTTATGTCCTGTAATTCAAATAACATGAGATCTGATCTTGTGTAATAGAAGGGAAAGTCGGACAAAATTATACACAATTGCTTTCTATTAATAGATCATACTTTTTTTTTTTAGTGTGAACTTATTAGAGCTGTTTCTAACCAAATAACCAAAATTTTGAAATATATATGCAGGCATCTCAATATGGTATAATAGGGATTACCTTACTCTCTCATTGGTATGAGCCGGCCTCTCAAAGGAAAGCAGATATTGATGCTGCACAACGAGGCCTCGACTTCATGTTTGGATGGTAAAGATTAAACTTAACTATTCGTAGCTCAACACTAACAGATAAGAATATGATCATTAAATTTTTTGAAAATATTAATTAATTTTTCTAAAAATGTAATGTAAACTTCATTTGATTTTGGTTCACTCTATGTTTTGAACTCAAGAAATAAGTTATGTAGATTTGCTGAATTCAAATGCAACTATATGCCATTTGTGCAAAGTAACATAGGAAATCAAAGAAGTCTAACACATTTTTTTTATTGCATCTGCATTTTGCATTTTTTTCTGTAGGTATATGGATCCAATCACAAAAGGAAACTATCCAAAAAGCATGCAATCTTTGGTTGGAAGCCGGCTACCAAAGTTCTCCAAAGAAGAATCCGAGGATCTTAAGGGGTCCTTCGATTTTCTTGGCCTAAACTATTACTCATCCTCTTATGCTGCTGATGCACCTCACTTACGCGATGCCAGACCCGCCATACAAACTGATTCTCTTATCAATGCTACGTGTAATTATCAAATTAAATATAAGCTTGCACTAGTTAATTATACATACTCTAAACTTTATATCTAATCATGCTTTATTATTTTTTCGATGACAGTTCATCGTGATGGCAAGCCCCTTGGTCCAATGGTATATATTTTATCATTCATTAGCATCTAATTTGCATATCAATGCCTCTCATTTGTAGCTTTTGTGTTCAAAAATATTATTTCTAAAATATAACGATAACCTTTTTTTTAATTATATGCAGTCTGCTTCAAGTTGGTTATGCATTTATCCGCGAGGATTAGGACAACTGTTGCTTTACGTCAAGAATAAGTATAACAACCCCGTAATTTACATTACCGAAAATGGTAATAACTCTATTATACTTCTTATTCTCTAAGATATTATATGCATAATACTGAAACTTTAATAAAGCTAATGAAAGTGACAAAATCAATTGATAGGTCGCGATGAGTTCAACGATCCAAAACTGTCGCTTGAAGAATCCCTTTTAGATACTTACAGAATCGATTACTTTTACCGTCATCTTTATTATGTTCAAGCTGCAATCAGGTAACTAATGCTTTATCAGAAAAACCTCTAATTAAGTTTTAGCTACTGATTTATAGGTTTTATACATATAAATTCACAATGCATCTTTTCTACCCTTCATTTTTACATTTACCTATTATTGACCTTTATGAATAACAGGGAAGGTGTGAATGTAAAGGGATATTTTGCATGGTCATTGTTGGATAACCATGAATGGGAATCTGGCTTTAGCTTGCGATTTGGACTCGTATTTGTGGATTACAACGACGACTTGAAAAGACACCCAAAACTCTCGGCACACTGGTTCAAGAATTTCCTCAAAAAATCTTGCCAAGAAAAGTGTTCTCTTTAA
Protein sequence

>MS.gene003916.t1

MLLNLLHYSIFATFPISIGPYEGAAFEDGKGPSIWDTFTHEHPEKIRDGGNGDVADDAYHRYKEDIGIIKDLNMDAYRFSIAWSRVLPKGKLSGGVNREGINYYNNLIDEILANGLQPYVTLFHWDVPQALEDEYDGFLSPRIVDDFRDYADLCYKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCSDWLNLNCTGGDSGTEPYLAAHYQLLAHAAATKLYKTKYQASQYGIIGITLLSHWYEPASQRKADIDAAQRGLDFMFGWYMDPITKGNYPKSMQSLVGSRLPKFSKEESEDLKGSFDFLGLNYYSSSYAADAPHLRDARPAIQTDSLINATFHRDGKPLGPMSASSWLCIYPRGLGQLLLYVKNKYNNPVIYITENGRDEFNDPKLSLEESLLDTYRIDYFYRHLYYVQAAIREGVNVKGYFAWSLLDNHEWESGFSLRFGLVFVDYNDDLKRHPKLSAHWFKNFLKKSCQEKCSL