AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016938


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016938.t1 MTR_5g088980 72.067 179 50 0 4 182 76 254 1.93e-85 258
MS.gene016938.t1 MTR_7g089080 50.279 179 85 2 5 182 113 288 9.41e-50 172
MS.gene016938.t1 MTR_5g023790 46.018 113 60 1 71 182 150 262 1.18e-29 112
MS.gene016938.t1 MTR_5g023790 46.018 113 60 1 71 182 150 262 1.93e-29 112
MS.gene016938.t1 MTR_5g023790 46.018 113 60 1 71 182 150 262 2.84e-29 112
MS.gene016938.t1 MTR_8g074000 44.545 110 60 1 74 182 157 266 1.16e-27 107
MS.gene016938.t1 MTR_1g011470 40.945 127 68 4 58 182 131 252 9.31e-22 91.3
MS.gene016938.t1 MTR_3g088415 39.130 138 72 4 56 182 195 331 1.18e-21 90.9
MS.gene016938.t1 MTR_3g088415 39.130 138 72 4 56 182 195 331 1.64e-21 90.9
MS.gene016938.t1 MTR_3g088415 39.130 138 72 4 56 182 195 331 1.68e-21 91.3
MS.gene016938.t1 MTR_3g107970 42.593 108 59 3 77 182 198 304 1.10e-20 88.6
MS.gene016938.t1 MTR_2g102520 40.000 130 70 4 61 182 177 306 1.90e-19 85.1
MS.gene016938.t1 MTR_3g102520 40.566 106 59 2 81 182 79 184 3.28e-18 81.3
MS.gene016938.t1 MTR_3g102530 40.566 106 59 1 81 182 72 177 9.79e-18 79.7
MS.gene016938.t1 MTR_2g102550 38.519 135 77 5 53 182 125 258 1.61e-17 79.7
MS.gene016938.t1 MTR_1g018680 41.748 103 55 3 84 182 83 184 3.14e-17 78.6
MS.gene016938.t1 MTR_3g102510 44.565 92 49 2 93 182 74 165 6.67e-17 77.4
MS.gene016938.t1 MTR_3g060770 37.719 114 63 3 76 184 13 123 8.99e-17 76.3
MS.gene016938.t1 MTR_3g088405 37.963 108 64 3 77 182 228 334 1.24e-16 77.0
MS.gene016938.t1 MTR_3g061680 40.196 102 55 2 86 184 15 113 2.65e-16 75.1
MS.gene016938.t1 MTR_3g060400 40.206 97 52 2 91 184 26 119 1.45e-15 73.2
MS.gene016938.t1 MTR_3g460990 40.206 97 52 2 91 184 26 119 1.45e-15 73.2
MS.gene016938.t1 MTR_5g011900 37.500 112 69 1 73 183 50 161 5.64e-15 70.9
MS.gene016938.t1 MTR_3g060820 39.175 97 53 2 91 184 20 113 6.60e-15 71.2
MS.gene016938.t1 MTR_5g088940 55.738 61 8 1 114 174 1 42 3.90e-14 64.3
MS.gene016938.t1 MTR_2g005960 32.800 125 69 4 73 182 102 226 3.43e-13 65.9
MS.gene016938.t1 MTR_6g045270 38.542 96 53 2 92 184 45 137 1.05e-12 65.5
MS.gene016938.t1 MTR_3g102560 47.222 72 37 1 112 182 86 157 6.07e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016938.t1 AT2G26760 50.296 169 74 3 14 182 61 219 1.39e-47 159
MS.gene016938.t1 AT4G37490 52.000 175 72 3 12 182 88 254 2.28e-47 160
MS.gene016938.t1 AT5G06150 47.191 178 94 0 5 182 94 271 4.68e-46 154
MS.gene016938.t1 AT5G06150 47.191 178 94 0 5 182 94 271 5.21e-45 154
MS.gene016938.t1 AT3G11520 47.059 170 70 4 14 182 98 248 3.00e-39 137
MS.gene016938.t1 AT3G11520 47.059 170 70 4 14 182 98 248 3.43e-39 138
MS.gene016938.t1 AT1G34460 46.667 120 64 0 50 169 226 345 5.70e-31 117
MS.gene016938.t1 AT1G34460 46.667 120 64 0 50 169 226 345 5.70e-31 117
MS.gene016938.t1 AT1G76310 49.541 109 53 2 76 182 160 268 1.15e-30 114
MS.gene016938.t1 AT1G34460 47.934 121 54 1 49 169 237 348 1.16e-30 116
MS.gene016938.t1 AT1G34460 42.537 134 77 0 36 169 201 334 1.28e-30 116
MS.gene016938.t1 AT1G34460 47.934 121 54 1 49 169 237 348 1.37e-30 116
MS.gene016938.t1 AT1G34460 47.934 121 54 1 49 169 237 348 1.37e-30 116
MS.gene016938.t1 AT1G34460 47.934 121 54 1 49 169 237 348 1.37e-30 116
MS.gene016938.t1 AT1G34460 47.934 121 54 1 49 169 237 348 1.37e-30 116
MS.gene016938.t1 AT1G34460 42.537 134 77 0 36 169 215 348 1.55e-30 116
MS.gene016938.t1 AT1G76310 49.541 109 53 2 76 182 160 268 3.86e-30 114
MS.gene016938.t1 AT1G76310 49.541 109 53 2 76 182 160 268 5.24e-30 114
MS.gene016938.t1 AT1G76310 49.541 109 53 2 76 182 160 268 5.98e-30 114
MS.gene016938.t1 AT1G20610 44.444 108 59 1 76 182 160 267 9.73e-29 110
MS.gene016938.t1 AT1G16330 42.574 101 57 1 83 182 385 485 6.38e-24 98.2
MS.gene016938.t1 AT1G44110 42.857 112 61 3 73 182 175 285 4.70e-23 95.5
MS.gene016938.t1 AT4G35620 50.926 108 52 1 76 182 155 262 7.43e-23 94.0
MS.gene016938.t1 AT4G35620 50.926 108 52 1 76 182 155 262 9.22e-23 94.4
MS.gene016938.t1 AT1G77390 43.119 109 59 3 76 182 162 269 1.46e-21 90.9
MS.gene016938.t1 AT2G17620 46.491 114 60 1 70 182 148 261 3.61e-21 89.7
MS.gene016938.t1 AT1G47220 45.045 111 56 3 78 184 42 151 7.33e-21 88.2
MS.gene016938.t1 AT5G43080 37.500 152 91 4 34 182 28 178 7.54e-21 88.2
MS.gene016938.t1 AT5G43080 37.500 152 91 4 34 182 28 178 7.75e-21 88.2
MS.gene016938.t1 AT1G80370 43.220 118 64 3 67 182 172 288 8.74e-21 89.0
MS.gene016938.t1 AT1G47230 37.226 137 81 3 50 182 53 188 1.49e-20 87.8
MS.gene016938.t1 AT5G11300 40.000 125 72 3 60 182 140 263 2.74e-20 87.4
MS.gene016938.t1 AT5G11300 40.000 125 72 3 60 182 140 263 3.37e-20 87.0
MS.gene016938.t1 AT1G47210 44.037 109 56 3 78 182 88 195 6.24e-20 85.9
MS.gene016938.t1 AT1G47210 42.453 106 56 3 78 179 88 192 2.66e-19 81.3
MS.gene016938.t1 AT1G47230 36.957 138 81 4 50 182 53 189 7.19e-19 83.2
MS.gene016938.t1 AT5G25380 39.450 109 63 3 76 182 157 264 8.15e-18 80.5
MS.gene016938.t1 AT5G25380 39.450 109 63 3 76 182 163 270 9.10e-18 80.5
MS.gene016938.t1 AT5G25380 39.450 109 63 3 76 182 157 264 9.40e-18 80.1
MS.gene016938.t1 AT5G25380 39.450 109 63 3 76 182 157 264 9.40e-18 80.1
MS.gene016938.t1 AT1G15570 42.593 108 59 3 77 182 173 279 3.67e-17 78.6

Find 27 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTCAGATAGAAAGTTTC+TGG 0.200703 5.1:+70436992 None:intergenic
CTCCTCTTAGCCGTTATATC+TGG 0.320074 5.1:+70437067 None:intergenic
TTAGCACCTTGTGATGCATT+TGG 0.341745 5.1:+70437733 None:intergenic
AGAGAGGAATTTCAACTTGT+TGG 0.397326 5.1:-70436929 MS.gene016938:CDS
AATTACTGAACTACAAGCTT+TGG 0.414807 5.1:+70437486 None:intergenic
TGCTGATAAGGATAATGAAT+TGG 0.420062 5.1:-70437293 MS.gene016938:CDS
AGTAATTTCAGCTAGAAGAA+AGG 0.442539 5.1:-70437470 MS.gene016938:intron
ACAAGCTTTGGCATTTCTCC+TGG 0.456328 5.1:+70437498 None:intergenic
ACAAGTTGAAATTCCTCTCT+AGG 0.464310 5.1:+70436932 None:intergenic
TTCTTGTGGAATTGTGCTAA+AGG 0.481895 5.1:-70437338 MS.gene016938:CDS
GAACATTGATACTGCTGATA+AGG 0.486849 5.1:-70437305 MS.gene016938:CDS
TGTCAAATTCAGGCTTCTTG+TGG 0.503060 5.1:-70437352 MS.gene016938:intron
GGCTTCACAGCCAGATATAA+CGG 0.509060 5.1:-70437077 MS.gene016938:CDS
GGAGATCAATTCTTGTTGAC+TGG 0.513087 5.1:-70437048 MS.gene016938:CDS
TGTACGAGTACATGATTACA+TGG 0.521386 5.1:-70437098 MS.gene016938:CDS
GAGCTGATGACAATAACTTC+AGG 0.522150 5.1:+70437589 None:intergenic
AGCTTTGGCATTTCTCCTGG+TGG 0.529215 5.1:+70437501 None:intergenic
ACAATAACTTCAGGTACTGT+AGG 0.532942 5.1:+70437598 None:intergenic
TGACTCTTAATATTGTTGAC+CGG 0.533961 5.1:-70436976 MS.gene016938:CDS
GATCAATTCTTGTTGACTGG+TGG 0.548572 5.1:-70437045 MS.gene016938:CDS
CAAGTTGAAATTCCTCTCTA+GGG 0.572482 5.1:+70436933 None:intergenic
TGAAGAATATGCGGAAGTGA+AGG 0.574870 5.1:-70437563 MS.gene016938:CDS
AGCCAGATATAACGGCTAAG+AGG 0.588033 5.1:-70437069 MS.gene016938:CDS
CACAAAAGCTGTCCCTAGAG+AGG 0.594294 5.1:-70436945 MS.gene016938:CDS
CAGCTCAGATGAAGAATATG+CGG 0.656465 5.1:-70437572 MS.gene016938:CDS
AGTTAAGAGAAAGATCCACC+AGG 0.667171 5.1:-70437516 MS.gene016938:CDS
ATGTTTCCAAATGCATCACA+AGG 0.671375 5.1:-70437739 MS.gene016938:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAATTAGACA+TGG + chr5.1:70436972-70436991 None:intergenic 10.0%
!! ATATGAGAAATAGAAAATAA+TGG + chr5.1:70437193-70437212 None:intergenic 15.0%
!!! TTTTTTTTTTGTTTTGTTGC+AGG - chr5.1:70436983-70437002 MS.gene016938:CDS 20.0%
! TAGTTTCTTTCTTTCTTTGT+TGG - chr5.1:70437469-70437488 MS.gene016938:intron 25.0%
! TTGAATTTCATGAATGACTT+AGG - chr5.1:70437506-70437525 MS.gene016938:CDS 25.0%
!! TCTATTTCTCATATGTGATA+TGG - chr5.1:70437199-70437218 MS.gene016938:intron 25.0%
AATTACTGAACTACAAGCTT+TGG + chr5.1:70437148-70437167 None:intergenic 30.0%
AGTAATTTCAGCTAGAAGAA+AGG - chr5.1:70437161-70437180 MS.gene016938:intron 30.0%
CATGGAAATAGATAAACATG+AGG + chr5.1:70436954-70436973 None:intergenic 30.0%
GAACAATGTTTGTCAAATTC+AGG - chr5.1:70437269-70437288 MS.gene016938:CDS 30.0%
TGACTCTTAATATTGTTGAC+CGG - chr5.1:70437655-70437674 MS.gene016938:intron 30.0%
TGCTGATAAGGATAATGAAT+TGG - chr5.1:70437338-70437357 MS.gene016938:CDS 30.0%
! GTGATATGGTTTTCGATTTA+GGG - chr5.1:70437213-70437232 MS.gene016938:intron 30.0%
! TGTGATATGGTTTTCGATTT+AGG - chr5.1:70437212-70437231 MS.gene016938:intron 30.0%
!! TAGGGTTCTATTTTTCAATG+TGG - chr5.1:70437231-70437250 MS.gene016938:intron 30.0%
AGAGAGGAATTTCAACTTGT+TGG - chr5.1:70437702-70437721 MS.gene016938:intron 35.0%
AGAGTCAGATAGAAAGTTTC+TGG + chr5.1:70437642-70437661 None:intergenic 35.0%
ATGTTTCCAAATGCATCACA+AGG - chr5.1:70436892-70436911 MS.gene016938:CDS 35.0%
GAACATTGATACTGCTGATA+AGG - chr5.1:70437326-70437345 MS.gene016938:CDS 35.0%
TGTACGAGTACATGATTACA+TGG - chr5.1:70437533-70437552 MS.gene016938:CDS 35.0%
TTCTTGTGGAATTGTGCTAA+AGG - chr5.1:70437293-70437312 MS.gene016938:CDS 35.0%
! ACAAGTTGAAATTCCTCTCT+AGG + chr5.1:70437702-70437721 None:intergenic 35.0%
! ACAATAACTTCAGGTACTGT+AGG + chr5.1:70437036-70437055 None:intergenic 35.0%
! CAAGTTGAAATTCCTCTCTA+GGG + chr5.1:70437701-70437720 None:intergenic 35.0%
AGTTAAGAGAAAGATCCACC+AGG - chr5.1:70437115-70437134 MS.gene016938:intron 40.0%
CAGCTCAGATGAAGAATATG+CGG - chr5.1:70437059-70437078 MS.gene016938:CDS 40.0%
GAGCTGATGACAATAACTTC+AGG + chr5.1:70437045-70437064 None:intergenic 40.0%
GGAGATCAATTCTTGTTGAC+TGG - chr5.1:70437583-70437602 MS.gene016938:CDS 40.0%
TGAAGAATATGCGGAAGTGA+AGG - chr5.1:70437068-70437087 MS.gene016938:CDS 40.0%
TGTCAAATTCAGGCTTCTTG+TGG - chr5.1:70437279-70437298 MS.gene016938:CDS 40.0%
TTAGCACCTTGTGATGCATT+TGG + chr5.1:70436901-70436920 None:intergenic 40.0%
! GATCAATTCTTGTTGACTGG+TGG - chr5.1:70437586-70437605 MS.gene016938:CDS 40.0%
! TTTTGTTGCAGGACTCACTT+AGG - chr5.1:70436994-70437013 MS.gene016938:CDS 40.0%
!! CGCTTTTTACAAACTCTCTG+AGG - chr5.1:70437389-70437408 MS.gene016938:intron 40.0%
AGCCAGATATAACGGCTAAG+AGG - chr5.1:70437562-70437581 MS.gene016938:CDS 45.0%
CTCCTCTTAGCCGTTATATC+TGG + chr5.1:70437567-70437586 None:intergenic 45.0%
GGCTTCACAGCCAGATATAA+CGG - chr5.1:70437554-70437573 MS.gene016938:CDS 45.0%
! ACAAGCTTTGGCATTTCTCC+TGG + chr5.1:70437136-70437155 None:intergenic 45.0%
! CAGCTTTTGTGGACAAGAAC+CGG + chr5.1:70437677-70437696 None:intergenic 45.0%
CACAAAAGCTGTCCCTAGAG+AGG - chr5.1:70437686-70437705 MS.gene016938:intron 50.0%
! AGCTTTGGCATTTCTCCTGG+TGG + chr5.1:70437133-70437152 None:intergenic 50.0%
!! CTCTCTAGGGACAGCTTTTG+TGG + chr5.1:70437688-70437707 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 70436886 70437767 70436886 ID=MS.gene016938
chr5.1 mRNA 70436886 70437767 70436886 ID=MS.gene016938.t1;Parent=MS.gene016938
chr5.1 exon 70437740 70437767 70437740 ID=MS.gene016938.t1.exon1;Parent=MS.gene016938.t1
chr5.1 CDS 70437740 70437767 70437740 ID=cds.MS.gene016938.t1;Parent=MS.gene016938.t1
chr5.1 exon 70437471 70437637 70437471 ID=MS.gene016938.t1.exon2;Parent=MS.gene016938.t1
chr5.1 CDS 70437471 70437637 70437471 ID=cds.MS.gene016938.t1;Parent=MS.gene016938.t1
chr5.1 exon 70437243 70437362 70437243 ID=MS.gene016938.t1.exon3;Parent=MS.gene016938.t1
chr5.1 CDS 70437243 70437362 70437243 ID=cds.MS.gene016938.t1;Parent=MS.gene016938.t1
chr5.1 exon 70436886 70437125 70436886 ID=MS.gene016938.t1.exon4;Parent=MS.gene016938.t1
chr5.1 CDS 70436886 70437125 70436886 ID=cds.MS.gene016938.t1;Parent=MS.gene016938.t1
Gene Sequence

>MS.gene016938

ATGCTAATGTTTCCAAATGCATCACAAGGTGCTAATTTTTTAATTTTATTAAAATTAAATTATTACCTCATGTTTATCTATTTCCATGTCTAATTTTTTTTTTTTTTGTTTTGTTGCAGGACTCACTTAGGTGCTATTGCTCAGAAGCCTACAGTACCTGAAGTTATTGTCATCAGCTCAGATGAAGAATATGCGGAAGTGAAGGAGAAACAAGTTGTTAAAAGAAGAAAGTTAAGAGAAAGATCCACCAGGAGAAATGCCAAAGCTTGTAGTTCAGTAATTTCAGCTAGAAGAAAGGTTTTTTCCATTATTTTCTATTTCTCATATGTGATATGGTTTTCGATTTAGGGTTCTATTTTTCAATGTGGACTAATAATAAGTATGAACAATGTTTGTCAAATTCAGGCTTCTTGTGGAATTGTGCTAAAGGATTTAGTTGTGAACATTGATACTGCTGATAAGGATAATGAATTGGCTGTAACTGAGTACGTTGATGACATCTACGCTTTTTACAAACTCTCTGAGGTAATGTTTTATGCTTTGCTTTGATTTGTTTTGTGTTCTTTCTTTATATATATGTCTCTAGTTTCTTTCTTTCTTTGTTGGCATTCTCTAATGTTTTGAATTTCATGAATGACTTAGGTTGATGTACGAGTACATGATTACATGGCTTCACAGCCAGATATAACGGCTAAGAGGAGATCAATTCTTGTTGACTGGTGGAATGACGTGCATCGAACATTTAAACTCATGCCAGAAACTTTCTATCTGACTCTTAATATTGTTGACCGGTTCTTGTCCACAAAAGCTGTCCCTAGAGAGGAATTTCAACTTGTTGGCATAAGCTCAATGCTTATAGCATCAAAATATGAAGCGAAATGA

Protein sequence

>MS.gene016938.t1

MLMFPNASQGAIAQKPTVPEVIVISSDEEYAEVKEKQVVKRRKLRERSTRRNAKACSSVISARRKASCGIVLKDLVVNIDTADKDNELAVTEYVDDIYAFYKLSEVDVRVHDYMASQPDITAKRRSILVDWWNDVHRTFKLMPETFYLTLNIVDRFLSTKAVPREEFQLVGISSMLIASKYEAK