AlfalfaGEDB | MS.gene23576

M. sativa cultivar XinJiangDaYe / MS.gene23576

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08263.15 PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23576.t1	XP_003627718.1	97.9	140	2	1	1	140	1	139	1.30E-70	276.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23576.t1	Q00874	61.0	141	54	1	1	140	5	145	9.9e-43	175.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23576.t1	G7LEF9	97.9	140	2	1	1	140	1	139	9.4e-71	276.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052169	MS.gene23576	0.822946	1.80E-53	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene001050	MS.gene23576	PPI
MS.gene001051	MS.gene23576	PPI
MS.gene001053	MS.gene23576	PPI
MS.gene001054	MS.gene23576	PPI
MS.gene001056	MS.gene23576	PPI
MS.gene001968	MS.gene23576	PPI
MS.gene024831	MS.gene23576	PPI
MS.gene024836	MS.gene23576	PPI
MS.gene024840	MS.gene23576	PPI
MS.gene051275	MS.gene23576	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23576.t1	MTR_8g036920	98.634	366	4	1	1	366	1	365	0.0	720
MS.gene23576.t1	MTR_3g027940	69.648	369	109	2	1	366	1	369	0.0	523
MS.gene23576.t1	MTR_2g098250	62.953	359	128	3	12	366	15	372	1.65e-154	439
MS.gene23576.t1	MTR_4g065850	55.114	352	155	2	15	366	16	364	4.86e-135	390
MS.gene23576.t1	MTR_4g124460	48.378	370	170	3	2	366	4	357	6.07e-117	343
MS.gene23576.t1	MTR_4g065830	49.180	366	161	4	2	366	7	348	5.50e-112	331
MS.gene23576.t1	MTR_7g023730	33.609	363	221	6	5	366	15	358	4.87e-59	195
MS.gene23576.t1	MTR_8g076360	35.694	353	222	3	15	366	22	370	1.58e-55	186
MS.gene23576.t1	MTR_8g076410	35.977	353	221	3	15	366	22	370	2.24e-55	186
MS.gene23576.t1	MTR_1g115225	34.341	364	202	8	23	358	34	388	6.89e-55	190

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23576.t1	AT3G12610	62.568	366	136	1	1	365	5	370	4.88e-158	448
MS.gene23576.t1	AT3G20820	61.429	350	134	1	17	366	14	362	1.04e-156	445
MS.gene23576.t1	AT5G12940	59.722	360	144	1	7	366	11	369	1.70e-153	437
MS.gene23576.t1	AT5G23400	34.783	345	188	7	23	339	31	366	4.24e-56	193
MS.gene23576.t1	AT5G23400	29.640	361	173	6	84	366	227	584	2.10e-32	128
MS.gene23576.t1	AT5G23400	33.553	152	100	1	190	340	95	246	1.65e-15	78.2
MS.gene23576.t1	AT2G26380	34.971	346	185	9	18	336	24	356	4.48e-51	177
MS.gene23576.t1	AT2G26380	33.047	233	126	3	136	340	85	315	5.39e-27	112
MS.gene23576.t1	AT2G26380	32.057	209	115	6	174	355	75	283	4.79e-17	82.8
MS.gene23576.t1	AT1G33600	33.235	340	211	8	5	339	8	336	1.98e-49	173

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Find 119 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTCCGGCGACATTCC+CGG	0.352868	8.4:-61107712	MS.gene23576:CDS
AACTGACGCCGTGCCAGCCA+CGG	0.541349	8.4:+61107968	None:intergenic
AAGAGTGCTGCCCGGTCTGA+TGG	0.448061	8.4:+61108060	None:intergenic
AAGGTAACTCACCGGAGATT+TGG	0.348552	8.4:+61107569	None:intergenic
AAGGTCAAGATGCATTAAAC+CGG	0.523533	8.4:+61107600	None:intergenic
AAGTGTGAGTAATGAAAGTG+AGG	0.526263	8.4:+61108125	None:intergenic
AATATCGGAAGGTAACTCAC+CGG	0.666314	8.4:+61107561	None:intergenic
AATATTACCGGGAATGTCGC+CGG	0.523894	8.4:+61107705	None:intergenic
AATCCTGCCCGCCATCAGAC+CGG	0.493642	8.4:-61108071	MS.gene23576:CDS
AATCGACATCGGAATCTCGC+CGG	0.605139	8.4:+61107633	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAAGCAATAACCATCTTTG+TGG	-	chr8.4:61108038-61108057	MS.gene23576:CDS	30.0%
GTTATCATCAGCTAAGTTTA+TGG	-	chr8.4:61108004-61108023	MS.gene23576:CDS	30.0%
TCATCAAGTTTACAAATCTC+CGG	+	chr8.4:61107351-61107370	None:intergenic	30.0%
TTCGACTCTGTTAAGTAATA+CGG	-	chr8.4:61107854-61107873	MS.gene23576:CDS	30.0%
AAGGTCAAGATGCATTAAAC+CGG	+	chr8.4:61107589-61107608	None:intergenic	35.0%
AAGTGTGAGTAATGAAAGTG+AGG	+	chr8.4:61107064-61107083	None:intergenic	35.0%
AGCTAAGTTTATGGGTCATT+TGG	-	chr8.4:61108013-61108032	MS.gene23576:CDS	35.0%
ATTTCCAATCAGCAACAACA+AGG	+	chr8.4:61107383-61107402	None:intergenic	35.0%
CTGAAGCTTTCCAATATTAC+CGG	+	chr8.4:61107496-61107515	None:intergenic	35.0%
CTGTTAAGTAATACGGGTAT+GGG	-	chr8.4:61107861-61107880	MS.gene23576:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	61107054	61108154	61107054	ID=MS.gene23576
chr8.4	mRNA	61107054	61108154	61107054	ID=MS.gene23576.t1;Parent=MS.gene23576
chr8.4	exon	61107054	61108154	61107054	ID=MS.gene23576.t1.exon1;Parent=MS.gene23576.t1
chr8.4	CDS	61107054	61108154	61107054	ID=cds.MS.gene23576.t1;Parent=MS.gene23576.t1

Gene Sequence

>MS.gene23576

ATGGCTTCCTCACTTTCATTACTCACACTTCTTCTTCTCCTCACCGTCATCTCCACCGTTAAATCCTGCCCGCCATCAGACCGGGCAGCACTCTTAGCCTTCAAATCTGCCCTAACAGAGCCCAACCTCGGCATCTTCAACACTTGGTCAGGCTACGACTGTTGCCGTGGCTGGCACGGCGTCAGTTGCAACCCCACCACCTGGCGTGTTACTGACATTAACCTCCGCGGCGACTCCGAAGACCCCATCTTCCAAAACCTCACTCATTCCGGTGACATGACCGGAGAAATCTCGCCGGAGATTTGTAAACTTGATGAACTCACCACCCTTGTTGTTGCTGATTGGAAATCCATCTCCGGTGAAATACCTTCCTGCATTACATCTCTCTCTTCGCTAAGAATTCTTGACCTCACCGGAAACAAAATCTCCGGCGACATTCCCGGTAATATTGGAAAGCTTCAGCATCTCACTGTTTTGAACCTCGCTGACAATGCTATCTCCGGCGAGATTCCGATGTCGATTGTGAGAATCTCCGGTTTAATGCATCTTGACCTTGCCGGAAACCAAATCTCCGGTGAGTTACCTTCCGATATTGGAAAACTCCGGCGACTAAGCCGCGCGTTATTTAGCCGAAACCAACTCACGGGTTCAATTCCTGATTCGGTTTTGAAAATGAACCGGCTTGCGGATTTGGATTTATCTATGAACCGGATAACCGGTTCGATTCCGGCTCGGATAGGAAAAATGCGGGTTTTGTCTACTTTGAAACTTGATGGTAACTCTATGACGGGTCAAATACCTTCGACTCTGTTAAGTAATACGGGTATGGGTATTTTGAATTTGAGTAGAAACGGGTTTGAAGGAACCATACCCGATGTATTCGGATCAAAATCGTATTTTATGGTTCTTGATTTGTCGTTTAATAATTTAACGGGTCGGATACCCGGTTCGTTATCATCAGCTAAGTTTATGGGTCATTTGGATATAAGCAATAACCATCTTTGTGGAACTATACCAATCGGGTCACCGTTTGATCATCTTGATGCGGCGTCGTTTAGTAATAATGATTGTTTATGTGGAAACCCATTGAAGACTTGTTAA

Protein sequence

>MS.gene23576.t1

MASSLSLLTLLLLLTVISTVKSCPPSDRAALLAFKSALTEPNLGIFNTWSGYDCCRGWHGVSCNPTTWRVTDINLRGDSEDPIFQNLTHSGDMTGEISPEICKLDELTTLVVADWKSISGEIPSCITSLSSLRILDLTGNKISGDIPGNIGKLQHLTVLNLADNAISGEIPMSIVRISGLMHLDLAGNQISGELPSDIGKLRRLSRALFSRNQLTGSIPDSVLKMNRLADLDLSMNRITGSIPARIGKMRVLSTLKLDGNSMTGQIPSTLLSNTGMGILNLSRNGFEGTIPDVFGSKSYFMVLDLSFNNLTGRIPGSLSSAKFMGHLDISNNHLCGTIPIGSPFDHLDAASFSNNDCLCGNPLKTC