AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020289

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020289.t1 MTR_7g095710 98.207 948 17 0 1 948 115 1062 0.0 1919
MS.gene020289.t1 MTR_6g016470 68.617 940 280 6 1 933 83 1014 0.0 1284
MS.gene020289.t1 MTR_4g008170 57.524 937 380 9 1 929 159 1085 0.0 1022
MS.gene020289.t1 MTR_4g008170 57.082 946 377 11 1 929 159 1092 0.0 1007
MS.gene020289.t1 MTR_2g038310 55.662 936 400 6 1 929 146 1073 0.0 999
MS.gene020289.t1 MTR_2g038310 56.140 855 360 6 1 848 146 992 0.0 902
MS.gene020289.t1 MTR_4g008650 47.436 936 447 15 3 925 108 1011 0.0 823
MS.gene020289.t1 MTR_8g090125 46.878 945 453 15 2 930 63 974 0.0 812
MS.gene020289.t1 MTR_4g096990 48.443 931 444 11 3 925 110 1012 0.0 811
MS.gene020289.t1 MTR_3g103070 47.863 936 447 13 2 925 110 1016 0.0 804
MS.gene020289.t1 MTR_5g015590 47.803 933 449 12 3 925 110 1014 0.0 801
MS.gene020289.t1 MTR_8g013780 67.414 580 176 7 363 938 1 571 0.0 792
MS.gene020289.t1 MTR_0005s0140 45.484 941 464 14 5 928 28 936 0.0 781
MS.gene020289.t1 MTR_4g043690 45.387 943 466 14 3 928 111 1021 0.0 781
MS.gene020289.t1 MTR_3g103070 48.792 869 404 13 69 925 1 840 0.0 761
MS.gene020289.t1 MTR_2g105830 54.762 714 310 9 1 711 148 851 0.0 731
MS.gene020289.t1 MTR_5g015590 47.194 873 417 13 3 859 110 954 0.0 724
MS.gene020289.t1 MTR_8g446010 41.738 932 494 15 9 925 70 967 0.0 671
MS.gene020289.t1 MTR_8g045070 40.880 932 479 15 9 925 70 944 0.0 651
MS.gene020289.t1 MTR_4g008650 46.438 758 365 14 3 751 108 833 0.0 640
MS.gene020289.t1 MTR_5g015590 46.358 755 371 11 3 751 110 836 0.0 605
MS.gene020289.t1 MTR_5g015590 44.921 699 351 11 3 695 110 780 1.64e-172 522
MS.gene020289.t1 MTR_8g013810 71.585 366 101 1 1 363 82 447 4.13e-172 511
MS.gene020289.t1 MTR_5g097270 32.337 937 559 20 2 916 114 997 1.39e-141 449
MS.gene020289.t1 MTR_4g014480 29.989 947 575 20 1 925 69 949 1.19e-116 381
MS.gene020289.t1 MTR_7g100110 31.770 853 455 24 31 789 47 866 7.91e-100 337
MS.gene020289.t1 MTR_8g027160 30.222 857 496 21 31 807 35 869 9.83e-95 320
MS.gene020289.t1 MTR_8g027160 28.902 993 584 29 31 924 35 1004 1.04e-94 323
MS.gene020289.t1 MTR_3g103270 28.455 984 568 26 17 903 41 985 1.21e-94 322
MS.gene020289.t1 MTR_2g105690 63.348 221 76 1 714 929 22 242 4.42e-92 293
MS.gene020289.t1 MTR_8g027160 29.475 743 423 20 31 694 35 755 1.18e-69 247
MS.gene020289.t1 MTR_8g027160 29.341 743 424 20 31 694 35 755 5.91e-69 245
MS.gene020289.t1 MTR_4g116950 25.350 714 419 21 38 737 44 657 1.76e-49 190
MS.gene020289.t1 MTR_7g117500 24.381 767 461 24 31 781 43 706 4.25e-49 189
MS.gene020289.t1 MTR_4g127710 23.810 714 444 19 31 737 39 659 9.89e-47 181
MS.gene020289.t1 MTR_4g127710 23.810 714 444 19 31 737 39 659 1.31e-46 181
MS.gene020289.t1 MTR_3g108800 24.051 711 446 18 31 737 35 655 1.39e-46 181
MS.gene020289.t1 MTR_3g108800 24.051 711 446 18 31 737 36 656 1.39e-46 181
MS.gene020289.t1 MTR_2g449840 23.973 730 438 22 31 745 37 664 1.58e-46 181
MS.gene020289.t1 MTR_7g117500 23.797 769 452 25 31 781 43 695 9.41e-46 178
MS.gene020289.t1 MTR_2g104860 24.205 723 441 22 33 745 36 661 2.51e-45 177
MS.gene020289.t1 MTR_8g089870 25.275 819 501 26 15 802 15 753 3.28e-45 176
MS.gene020289.t1 MTR_2g036650 23.001 713 452 16 31 737 41 662 5.30e-45 176
MS.gene020289.t1 MTR_4g107500 25.352 639 365 21 131 754 137 678 9.34e-45 175
MS.gene020289.t1 MTR_8g063880 23.422 713 448 20 31 737 36 656 9.39e-45 175
MS.gene020289.t1 MTR_1g009760 22.880 743 466 21 10 737 9 659 1.01e-44 174
MS.gene020289.t1 MTR_1g009760 23.001 713 451 20 31 737 36 656 2.93e-44 174
MS.gene020289.t1 MTR_6g011310 25.585 641 361 22 131 754 135 676 4.20e-44 173
MS.gene020289.t1 MTR_6g011310 25.117 641 359 21 131 754 135 671 5.91e-43 169
MS.gene020289.t1 MTR_1g009720 22.937 715 449 21 31 737 36 656 9.48e-43 169
MS.gene020289.t1 MTR_8g006790 22.659 737 444 21 31 745 38 670 3.18e-41 164
MS.gene020289.t1 MTR_4g094695 23.983 688 405 19 59 740 362 937 6.03e-26 115
MS.gene020289.t1 MTR_3g097560 24.468 752 413 31 30 700 166 843 9.81e-25 112
MS.gene020289.t1 MTR_2g090610 21.387 865 516 29 52 783 34 867 2.78e-19 94.0
MS.gene020289.t1 MTR_2g090610 21.387 865 516 29 52 783 34 867 2.90e-19 94.4
MS.gene020289.t1 MTR_2g035840 30.811 185 94 2 558 742 788 938 5.41e-18 90.1
MS.gene020289.t1 MTR_8g079250 27.568 185 100 2 558 742 788 938 2.41e-16 84.7
MS.gene020289.t1 MTR_5g010420 31.250 160 76 2 560 719 781 906 9.29e-16 82.8
MS.gene020289.t1 MTR_8g012390 29.730 185 96 2 558 742 759 909 1.82e-14 78.6
MS.gene020289.t1 MTR_4g008640 40.000 105 52 2 495 599 77 170 6.71e-14 71.2
MS.gene020289.t1 MTR_5g052190 50.000 66 32 1 437 502 282 346 2.44e-12 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020289.t1 AT3G63380 73.340 934 232 5 2 935 100 1016 0.0 1321
MS.gene020289.t1 AT3G22910 68.936 940 272 6 1 938 94 1015 0.0 1238
MS.gene020289.t1 AT4G29900 56.794 942 391 8 1 935 127 1059 0.0 1033
MS.gene020289.t1 AT4G29900 56.794 942 391 8 1 935 127 1059 0.0 1033
MS.gene020289.t1 AT5G57110 56.303 936 391 6 1 929 127 1051 0.0 1027
MS.gene020289.t1 AT5G57110 56.303 936 391 6 1 929 127 1051 0.0 1027
MS.gene020289.t1 AT5G57110 56.303 936 391 6 1 929 127 1051 0.0 1027
MS.gene020289.t1 AT3G21180 55.932 944 397 9 1 935 141 1074 0.0 995
MS.gene020289.t1 AT3G57330 48.453 937 434 14 1 925 106 1005 0.0 854
MS.gene020289.t1 AT3G57330 48.453 937 434 14 1 925 51 950 0.0 853
MS.gene020289.t1 AT2G41560 47.447 940 444 15 3 928 108 1011 0.0 825
MS.gene020289.t1 AT2G41560 47.553 940 443 16 3 928 6 909 0.0 825
MS.gene020289.t1 AT2G41560 47.553 940 443 16 3 928 6 909 0.0 825
MS.gene020289.t1 AT2G41560 47.553 940 443 16 3 928 6 909 0.0 825
MS.gene020289.t1 AT1G27770 47.701 935 449 11 3 925 48 954 0.0 805
MS.gene020289.t1 AT1G27770 47.701 935 449 11 3 925 111 1017 0.0 805
MS.gene020289.t1 AT1G27770 47.701 935 449 11 3 925 111 1017 0.0 805
MS.gene020289.t1 AT4G37640 48.081 938 440 11 3 925 109 1014 0.0 794
MS.gene020289.t1 AT2G22950 47.868 938 443 15 3 925 109 1015 0.0 792
MS.gene020289.t1 AT5G53010 35.398 904 493 14 1 872 153 997 5.27e-164 508
MS.gene020289.t1 AT1G27770 40.759 606 327 11 3 600 111 692 2.82e-117 382
MS.gene020289.t1 AT1G27770 56.958 309 128 2 622 925 635 943 8.36e-112 367
MS.gene020289.t1 AT5G53010 35.093 644 395 7 1 642 153 775 6.24e-112 363
MS.gene020289.t1 AT1G07810 31.523 847 484 22 31 806 47 868 5.21e-106 354
MS.gene020289.t1 AT1G07670 31.604 848 482 22 31 806 47 868 4.49e-105 351
MS.gene020289.t1 AT1G07670 31.604 848 482 22 31 806 47 868 4.49e-105 351
MS.gene020289.t1 AT4G00900 30.160 998 569 28 31 924 30 1003 1.57e-101 342
MS.gene020289.t1 AT4G00900 30.160 998 569 28 31 924 30 1003 1.57e-101 342
MS.gene020289.t1 AT1G10130 28.786 997 575 27 20 923 16 970 3.41e-94 320
MS.gene020289.t1 AT3G60330 24.288 737 424 24 31 745 37 661 1.30e-49 191
MS.gene020289.t1 AT3G60330 24.288 737 424 24 31 745 37 661 1.30e-49 191
MS.gene020289.t1 AT3G60330 24.152 737 425 24 31 745 37 661 2.92e-49 189
MS.gene020289.t1 AT3G60330 24.152 737 425 24 31 745 37 661 2.92e-49 189
MS.gene020289.t1 AT2G24520 24.473 711 443 19 31 737 16 636 1.26e-48 187
MS.gene020289.t1 AT2G24520 24.473 711 443 19 31 737 16 636 1.26e-48 187
MS.gene020289.t1 AT2G24520 24.473 711 443 19 31 737 79 699 1.52e-48 187
MS.gene020289.t1 AT1G17260 23.776 858 523 25 13 848 27 775 3.57e-48 186
MS.gene020289.t1 AT3G42640 24.561 741 420 25 31 745 37 664 3.74e-47 183
MS.gene020289.t1 AT2G18960 26.010 619 351 16 131 737 67 590 3.31e-44 173
MS.gene020289.t1 AT2G18960 26.010 619 351 16 131 737 67 590 3.31e-44 173
MS.gene020289.t1 AT2G18960 26.010 619 351 16 131 737 131 654 5.65e-44 173
MS.gene020289.t1 AT5G57350 24.167 720 420 21 38 737 42 655 3.60e-43 170
MS.gene020289.t1 AT5G57350 24.167 720 420 21 38 737 42 655 3.60e-43 170
MS.gene020289.t1 AT5G57350 24.167 720 420 21 38 737 42 655 3.60e-43 170
MS.gene020289.t1 AT2G07560 23.481 724 448 22 31 745 37 663 6.96e-43 169
MS.gene020289.t1 AT4G30190 23.977 709 435 18 38 737 41 654 8.65e-43 169
MS.gene020289.t1 AT1G80660 23.731 729 441 23 31 745 39 666 1.48e-42 168
MS.gene020289.t1 AT1G80660 23.731 729 441 23 31 745 39 666 1.60e-42 168
MS.gene020289.t1 AT5G62670 24.214 636 376 20 131 754 135 676 1.65e-42 168
MS.gene020289.t1 AT1G80660 23.731 729 441 23 31 745 39 666 2.14e-42 168
MS.gene020289.t1 AT1G80660 25.080 626 363 20 131 745 7 537 1.09e-41 165
MS.gene020289.t1 AT3G47950 24.111 647 363 21 131 754 139 680 1.17e-41 166
MS.gene020289.t1 AT3G47950 24.111 647 363 21 131 754 139 680 1.17e-41 166
MS.gene020289.t1 AT4G30190 23.004 739 438 19 38 737 41 687 2.56e-38 155
MS.gene020289.t1 AT2G24520 25.173 433 268 9 305 737 17 393 3.01e-33 137
MS.gene020289.t1 AT5G57350 25.282 443 255 12 305 737 17 393 2.00e-30 129
MS.gene020289.t1 AT4G11730 23.251 486 303 13 94 575 92 511 8.45e-22 102
MS.gene020289.t1 AT1G63440 31.892 185 92 3 558 742 787 937 6.46e-19 93.2
MS.gene020289.t1 AT5G23630 22.117 737 422 24 30 687 166 829 3.19e-18 90.9
MS.gene020289.t1 AT5G44790 31.892 185 92 2 558 742 798 948 2.40e-17 87.8
MS.gene020289.t1 AT4G30110 27.895 190 106 3 555 744 508 666 5.26e-16 83.6
MS.gene020289.t1 AT4G30110 27.895 190 106 3 555 744 508 666 5.26e-16 83.6
MS.gene020289.t1 AT2G19110 25.316 237 133 5 555 791 518 710 4.60e-13 74.3
MS.gene020289.t1 AT2G19110 25.316 237 133 5 555 791 518 710 4.60e-13 74.3
MS.gene020289.t1 AT2G19110 25.316 237 133 5 555 791 518 710 4.60e-13 74.3

Find 176 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTGTTTCTTGGAATTAT+TGG 0.097609 7.4:+13652789 MS.gene020289:CDS
GTTGAAACTGAATAAATTTC+TGG 0.116163 7.4:-13652359 None:intergenic
TAACAACAGTTCAACTTCTT+TGG 0.173261 7.4:+13652448 MS.gene020289:CDS
GTTGGGCTCCCTAAAATTTC+AGG 0.196891 7.4:-13651427 None:intergenic
AGAAGTTGAACTGTTGTTAA+AGG 0.219340 7.4:-13652444 None:intergenic
GACGATATCGGAACTTTCTT+TGG 0.231369 7.4:-13652268 None:intergenic
TTGAAGTCCTTAACTGAGTT+TGG 0.249267 7.4:+13650194 MS.gene020289:CDS
TCTTTGGGTGAATCTCATTA+TGG 0.261465 7.4:+13652464 MS.gene020289:CDS
AGCTGATATAGGACTTTCTA+TGG 0.273764 7.4:+13652224 MS.gene020289:CDS
ATGTGGGCAGTATTAGATTT+AGG 0.276374 7.4:+13651466 MS.gene020289:CDS
ACTTTCAGCTTGTGAAACTA+TGG 0.298547 7.4:+13651204 MS.gene020289:CDS
ACTTGTTTCTTGGAATTATT+GGG 0.300596 7.4:+13652790 MS.gene020289:CDS
AGAGGCGCGGTTCGACCTAT+TGG 0.309301 7.4:-13652549 None:intergenic
GCTGGTCTTTCCCTTGGTTT+TGG 0.316343 7.4:+13650407 MS.gene020289:CDS
TTCTTCTAGTGTTGTTAATT+CGG 0.316776 7.4:+13650966 MS.gene020289:CDS
TTTATTGCAGCAGTTTCTTC+TGG 0.323673 7.4:+13652414 MS.gene020289:CDS
AACAACAGTTCAACTTCTTT+GGG 0.329161 7.4:+13652449 MS.gene020289:CDS
CTTTGGCGACTTCGGTTCCT+TGG 0.332634 7.4:-13652251 None:intergenic
TGGAACAACTCAAGAACTTT+TGG 0.332942 7.4:-13651346 None:intergenic
CTTCCAATGATTCCCTTTGT+TGG 0.333542 7.4:-13650254 None:intergenic
ACAAAATGTAAAAGACCTTT+TGG 0.340140 7.4:-13650338 None:intergenic
AACGAAGTGGTGTTGCTATA+AGG 0.341059 7.4:+13651557 MS.gene020289:CDS
ACATGATGAGAAAGGGAATA+AGG 0.342848 7.4:+13651006 MS.gene020289:CDS
TCCACAGCTTTCTTAGTGTT+TGG 0.343135 7.4:-13651892 None:intergenic
ATTCATAACATCGTTTATAT+CGG 0.348604 7.4:-13651047 None:intergenic
ATCATTCTTCTTGTTTGTGC+TGG 0.353362 7.4:+13650389 MS.gene020289:CDS
TTTCCAACAAAGGGAATCAT+TGG 0.361980 7.4:+13650251 MS.gene020289:CDS
TATTGAGTCCTATCCCTTGT+TGG 0.366939 7.4:-13651366 None:intergenic
GAAGGTGTTGGACATGTTCT+TGG 0.367950 7.4:+13650224 MS.gene020289:CDS
CACATAATGTTTGTAATAAG+AGG 0.371774 7.4:-13652567 None:intergenic
AAAGTTGAAGTTGTGAGAAA+TGG 0.379054 7.4:+13650575 MS.gene020289:CDS
TTTCTCATCATGTGAGTTAC+CGG 0.383047 7.4:-13650996 None:intergenic
TTCTTAGTGTTTGGTCTGCA+TGG 0.388331 7.4:-13651883 None:intergenic
GTCCACTTGCAACGAATAAC+CGG 0.388586 7.4:-13650693 None:intergenic
AAGATTAACATGGGAGCAAT+GGG 0.392316 7.4:+13652872 MS.gene020289:CDS
TGAGTATAGCTTTCTCCGTT+GGG 0.404152 7.4:-13651444 None:intergenic
GTTTGTGCTGGTCTTTCCCT+TGG 0.406959 7.4:+13650401 MS.gene020289:CDS
ACTGAGTTTGGAGGAGTTGA+AGG 0.409047 7.4:+13650206 MS.gene020289:CDS
CATCAAATGCTTAGAGAAGA+TGG 0.409715 7.4:+13651826 MS.gene020289:CDS
CATCCCTGAGGGTTTGCCAT+TGG 0.412027 7.4:+13651117 MS.gene020289:CDS
AGTTGAATTCGTTGAAAACT+TGG 0.412550 7.4:-13652719 None:intergenic
TCCTTAGCAGTTCCACCATC+AGG 0.413653 7.4:-13652833 None:intergenic
GAGCAATGGGGAATTTGTAT+TGG 0.414811 7.4:+13652885 MS.gene020289:CDS
CTTGGAACATTTCCAACAAA+GGG 0.418051 7.4:+13650242 MS.gene020289:CDS
TTGGAATTGCAGTTGTTTCA+TGG 0.420704 7.4:+13652904 MS.gene020289:CDS
TCGAGACGGCTCGAGTTGTT+CGG 0.422005 7.4:+13650290 MS.gene020289:CDS
ATCAATCTTCAATGTAAGTA+AGG 0.423054 7.4:+13652656 MS.gene020289:CDS
AAGGAAGGTTTATCTGAAAC+AGG 0.426540 7.4:-13652954 None:intergenic
TTCAAGACCAAGACAAAACT+TGG 0.430220 7.4:-13651290 None:intergenic
GATCATATAATCAATATCTT+CGG 0.433658 7.4:-13651785 None:intergenic
TATGGATACACTAGGTGCTT+TGG 0.436284 7.4:+13652482 MS.gene020289:CDS
CCATCAAAAGTTTGTCCATA+GGG 0.437671 7.4:-13652125 None:intergenic
GAAGATGGATTGACATTGCT+TGG 0.448536 7.4:+13651841 MS.gene020289:CDS
CCAGGGGAAGGTTGGTATGA+AGG 0.449354 7.4:+13650446 MS.gene020289:CDS
GAGAAACTTTATGTATTTGA+AGG 0.449643 7.4:+13652753 MS.gene020289:CDS
GGAACTTTCTTTGGCGACTT+CGG 0.449820 7.4:-13652259 None:intergenic
ACAACAGCGTTCACGTGCAT+TGG 0.450477 7.4:+13651593 MS.gene020289:CDS
ACCTCTTCGATTGGAAAGGT+TGG 0.454559 7.4:+13650923 MS.gene020289:CDS
AACTCCTCCAAACTCAGTTA+AGG 0.459202 7.4:-13650201 None:intergenic
AATAATGATCATGCAAATGC+TGG 0.461039 7.4:+13652012 MS.gene020289:CDS
GTTATCTGCACTGATAAAAC+AGG 0.462331 7.4:+13651238 MS.gene020289:CDS
ATGAGTATAGCTTTCTCCGT+TGG 0.465674 7.4:-13651445 None:intergenic
ATATTGATTATATGATCAAA+AGG 0.466009 7.4:+13651791 MS.gene020289:CDS
CTATACAGAGGAAGAGAGAA+TGG 0.471313 7.4:+13652065 MS.gene020289:CDS
GCCTTAAAAGAAGCTGATAT+AGG 0.472466 7.4:+13652213 MS.gene020289:CDS
ACAGCCAATGGCAAACCCTC+AGG 0.480432 7.4:-13651121 None:intergenic
GGGATAGGACTCAATACAAC+TGG 0.480556 7.4:+13651373 MS.gene020289:CDS
AAAGATTAACATGGGAGCAA+TGG 0.480899 7.4:+13652871 MS.gene020289:CDS
AAAATTGAGAGAACCATTCA+AGG 0.484847 7.4:+13651706 MS.gene020289:CDS
CTTCCTTCAGTTATACTAAG+TGG 0.485117 7.4:+13652970 MS.gene020289:CDS
TTTGGAGGAGTTGAAGGTGT+TGG 0.486880 7.4:+13650212 MS.gene020289:CDS
CTCTTATTACAAACATTATG+TGG 0.489817 7.4:+13652568 MS.gene020289:CDS
TACTCCGTTCTTCTTCGTCA+AGG 0.493088 7.4:-13651684 None:intergenic
AAGTCCTTAACTGAGTTTGG+AGG 0.494300 7.4:+13650197 MS.gene020289:CDS
GTGCAATGCTTAAGAAAGAA+AGG 0.495384 7.4:+13652147 MS.gene020289:CDS
GCTTGGTATCGTTGGCCTCA+AGG 0.496620 7.4:+13651858 MS.gene020289:CDS
GACAAGTTAACCTCTTCGAT+TGG 0.496811 7.4:+13650914 MS.gene020289:CDS
CAATGTAAGTAAGGAGGTAA+AGG 0.498336 7.4:+13652665 MS.gene020289:CDS
AGTTAAATGTTTCAACATGA+AGG 0.506064 7.4:-13651525 None:intergenic
ACTCCGTTCTTCTTCGTCAA+GGG 0.507433 7.4:-13651683 None:intergenic
GGTCATGTAGTTGCGGTCAC+AGG 0.513331 7.4:+13652168 MS.gene020289:CDS
ACTAGCAGCCATACCTTGAA+TGG 0.515056 7.4:-13651719 None:intergenic
GGCAGTATTAGATTTAGGTA+TGG 0.515789 7.4:+13651471 MS.gene020289:CDS
GGTATAAAAGAACATGGACC+AGG 0.517553 7.4:+13650428 MS.gene020289:CDS
CTTTCAGCTTGTGAAACTAT+GGG 0.519163 7.4:+13651205 MS.gene020289:CDS
AACATGGACCAGGGGAAGGT+TGG 0.519256 7.4:+13650438 MS.gene020289:CDS
TGAGAGAACCATTCAAGGTA+TGG 0.519915 7.4:+13651711 MS.gene020289:CDS
TTGTTAATTCGGTATTTCAC+CGG 0.522783 7.4:+13650977 MS.gene020289:CDS
GAGTTGTTCCAACAAGGGAT+AGG 0.522823 7.4:+13651358 MS.gene020289:CDS
GTGGAAACTTGCAAAGCTGC+AGG 0.523844 7.4:+13651910 MS.gene020289:CDS
TCCTGATGGTGGAACTGCTA+AGG 0.525298 7.4:+13652832 MS.gene020289:CDS
AGTGTCTATAAGCCTCCATC+AGG 0.527925 7.4:+13651397 MS.gene020289:CDS
CCTTCATACCAACCTTCCCC+TGG 0.528123 7.4:-13650446 None:intergenic
TATTGATTATATGATCAAAA+GGG 0.529809 7.4:+13651792 MS.gene020289:CDS
GATATGAAGCTTGAGCTAGA+AGG 0.531218 7.4:-13652596 None:intergenic
TTCCTTCAGTTATACTAAGT+GGG 0.531273 7.4:+13652971 MS.gene020289:CDS
GTGGTCGTTGCCATCCCTGA+GGG 0.533218 7.4:+13651106 MS.gene020289:CDS
AGAAACTTTATGTATTTGAA+GGG 0.534132 7.4:+13652754 MS.gene020289:CDS
TTGACATTGCTTGGTATCGT+TGG 0.535773 7.4:+13651850 MS.gene020289:CDS
TATTGTTCTTCAAATCCTGA+TGG 0.536690 7.4:+13652818 MS.gene020289:CDS
GTTAACCTCTTCGATTGGAA+AGG 0.543977 7.4:+13650919 MS.gene020289:CDS
TTACTCCATGAAAAGAATGA+TGG 0.544747 7.4:+13651159 MS.gene020289:CDS
AAAGGGAATAAGGAATTTCG+AGG 0.549749 7.4:+13651016 MS.gene020289:CDS
CGATAACATATTCACTGCTA+AGG 0.549853 7.4:+13651957 MS.gene020289:CDS
TTGTCGAGGCGTGCTTGTAA+CGG 0.550021 7.4:-13650896 None:intergenic
GATGTTGTGTCTCTCAAGAT+TGG 0.550678 7.4:+13650638 MS.gene020289:CDS
TCTTGGAACATTTCCAACAA+AGG 0.551511 7.4:+13650241 MS.gene020289:CDS
GTTCTTGAGTTGTTCCAACA+AGG 0.551602 7.4:+13651352 MS.gene020289:CDS
GTGGACGAATCTAGCATGAC+AGG 0.554098 7.4:+13650710 MS.gene020289:CDS
GGTAACTCACATGATGAGAA+AGG 0.554188 7.4:+13650998 MS.gene020289:CDS
GGTGATCAAATTCCAGCTGA+TGG 0.555778 7.4:+13650659 MS.gene020289:CDS
ATCCGGTTATTCGTTGCAAG+TGG 0.559045 7.4:+13650691 MS.gene020289:CDS
TTCTTGAGTTGTTCCAACAA+GGG 0.564125 7.4:+13651353 MS.gene020289:CDS
CTGATACAGAAAGATTAACA+TGG 0.570100 7.4:+13652862 MS.gene020289:CDS
GTCGTCTAAGATGACGATAT+CGG 0.571011 7.4:-13652280 None:intergenic
CAGATGCTTGTAACATCTGT+GGG 0.571667 7.4:+13650812 MS.gene020289:CDS
GTAACTCACATGATGAGAAA+GGG 0.572303 7.4:+13650999 MS.gene020289:CDS
ACAAACTATATTGATAGTAA+TGG 0.572318 7.4:+13651649 MS.gene020289:CDS
AGAGTGAGTGTCACAGCCAA+TGG 0.572339 7.4:-13651133 None:intergenic
CGAATTCAACTCAAGAAGCA+TGG 0.573309 7.4:+13652731 MS.gene020289:CDS
TGTTCTTCAAATCCTGATGG+TGG 0.574137 7.4:+13652821 MS.gene020289:CDS
GGGATGATCTTGCCATCACT+CGG 0.576371 7.4:-13652104 None:intergenic
AAAGAACATGGACCAGGGGA+AGG 0.576921 7.4:+13650434 MS.gene020289:CDS
GATGGCAAGATCATCCCCTA+TGG 0.578067 7.4:+13652110 MS.gene020289:CDS
GGACTTTCTATGGGAATCCA+AGG 0.578565 7.4:+13652234 MS.gene020289:CDS
ACCATCAAAAGTTTGTCCAT+AGG 0.578755 7.4:-13652126 None:intergenic
GTTGAAATTAAGATGATCAC+CGG 0.583984 7.4:+13651934 MS.gene020289:CDS
AGCGTTCACGTGCATTGGAA+AGG 0.585112 7.4:+13651598 MS.gene020289:CDS
AGCAGTGAATATGTTATCGC+CGG 0.592625 7.4:-13651953 None:intergenic
CAATGGCAAACCCTCAGGGA+TGG 0.594427 7.4:-13651116 None:intergenic
CAATAATTCCAAGAAACAAG+TGG 0.594925 7.4:-13652788 None:intergenic
TGCGGCTGCAGTCACTATTG+TGG 0.595274 7.4:+13651087 MS.gene020289:CDS
AGCTTTACTAAGCAAGCAAG+TGG 0.597015 7.4:-13652927 None:intergenic
CTTTGGCACTAGCAACAGAA+AGG 0.597226 7.4:+13652499 MS.gene020289:CDS
AAAGAAAGGTCATGTAGTTG+CGG 0.598089 7.4:+13652161 MS.gene020289:CDS
CGGAGAAAGCTATACTCATG+TGG 0.599631 7.4:+13651449 MS.gene020289:CDS
TCCGGCGCAAAAGTTGTCGA+TGG 0.604642 7.4:+13650782 MS.gene020289:CDS
AACTCAGAAAAGAAACGAAG+TGG 0.604819 7.4:+13651544 MS.gene020289:CDS
AGTCCCTTGACGAAGAAGAA+CGG 0.605537 7.4:+13651680 MS.gene020289:CDS
GCTGATATAGGACTTTCTAT+GGG 0.607580 7.4:+13652225 MS.gene020289:CDS
AACCCACTTAGTATAACTGA+AGG 0.608822 7.4:-13652973 None:intergenic
AGTCGATAAGATCCGAGTGA+TGG 0.608832 7.4:+13652092 MS.gene020289:CDS
TCGAAGAGGTTAACTTGTCG+AGG 0.612007 7.4:-13650910 None:intergenic
TCTCATTTGATTCAATGTCA+AGG 0.612008 7.4:-13651263 None:intergenic
TTAAAGGCTCAATCTCTACA+TGG 0.614114 7.4:-13650742 None:intergenic
TCAGGTTCAGATCCTGATGG+AGG 0.616155 7.4:-13651409 None:intergenic
GTAGTTGCGGTCACAGGCGA+CGG 0.616359 7.4:+13652174 MS.gene020289:CDS
AATCTTCAATGTAAGTAAGG+AGG 0.620069 7.4:+13652659 MS.gene020289:CDS
CAGCCAATGGCAAACCCTCA+GGG 0.622410 7.4:-13651120 None:intergenic
ATTTCAGGTTCAGATCCTGA+TGG 0.624957 7.4:-13651412 None:intergenic
ATTAGATTTAGGTATGGACA+TGG 0.627125 7.4:+13651477 MS.gene020289:CDS
TGTGGTCGTTGCCATCCCTG+AGG 0.627513 7.4:+13651105 MS.gene020289:CDS
TCCAACCTTTCCAATCGAAG+AGG 0.628030 7.4:-13650924 None:intergenic
GGAGAAAGCTATACTCATGT+GGG 0.631351 7.4:+13651450 MS.gene020289:CDS
AAGGCAATAGCAATAGAATG+TGG 0.631732 7.4:+13651976 MS.gene020289:CDS
AATGCTGGAGAAGTGGTTGA+AGG 0.633324 7.4:+13652027 MS.gene020289:CDS
TTGAAGTTGTGAGAAATGGA+AGG 0.633334 7.4:+13650579 MS.gene020289:CDS
GTATAAAAGAACATGGACCA+GGG 0.633871 7.4:+13650429 MS.gene020289:CDS
TCAGATGCTTGTAACATCTG+TGG 0.634658 7.4:+13650811 MS.gene020289:CDS
TTCTGTTGTGAGTATCGTTG+CGG 0.635865 7.4:+13651069 MS.gene020289:CDS
AATGTTTGTAATAAGAGGCG+CGG 0.641151 7.4:-13652562 None:intergenic
CATCAAAAGTTTGTCCATAG+GGG 0.646674 7.4:-13652124 None:intergenic
AATCTCATTATGGATACACT+AGG 0.650066 7.4:+13652474 MS.gene020289:CDS
GGGGAAGGTTGGTATGAAGG+TGG 0.653350 7.4:+13650449 MS.gene020289:CDS
ACCAAACACTAAGAAAGCTG+TGG 0.654204 7.4:+13651891 MS.gene020289:CDS
TCATGCAAATGCTGGAGAAG+TGG 0.656322 7.4:+13652020 MS.gene020289:CDS
CACTTAGTATAACTGAAGGA+AGG 0.659297 7.4:-13652969 None:intergenic
AATGATGGCAGATCATGCTA+TGG 0.667862 7.4:+13651174 MS.gene020289:CDS
TGATACAGAAAGATTAACAT+GGG 0.677705 7.4:+13652863 MS.gene020289:CDS
TATAAAAGAACATGGACCAG+GGG 0.683444 7.4:+13650430 MS.gene020289:CDS
GTGCAGATGATATTTCGAGA+CGG 0.692212 7.4:+13650276 MS.gene020289:CDS
ATATTGATAGTAATGGCGTG+AGG 0.714007 7.4:+13651656 MS.gene020289:CDS
TGAATTCAGAAACTATACAG+AGG 0.727231 7.4:+13652053 MS.gene020289:CDS
TTGGTCTGCATGGATCCTTG+AGG 0.732996 7.4:-13651873 None:intergenic
TGGCAGATCATGCTATGGTG+AGG 0.739078 7.4:+13651179 MS.gene020289:CDS
AGATTAACATGGGAGCAATG+GGG 0.752103 7.4:+13652873 MS.gene020289:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTGATTATATGATCAAA+AGG + chr7.4:13651791-13651810 MS.gene020289:CDS 15.0%
!!! TATTGATTATATGATCAAAA+GGG + chr7.4:13651792-13651811 MS.gene020289:CDS 15.0%
!! AGAAACTTTATGTATTTGAA+GGG + chr7.4:13652754-13652773 MS.gene020289:CDS 20.0%
!! ATTCATAACATCGTTTATAT+CGG - chr7.4:13651050-13651069 None:intergenic 20.0%
!! GATCATATAATCAATATCTT+CGG - chr7.4:13651788-13651807 None:intergenic 20.0%
!!! ACAAACTATATTGATAGTAA+TGG + chr7.4:13651649-13651668 MS.gene020289:CDS 20.0%
! AGTTAAATGTTTCAACATGA+AGG - chr7.4:13651528-13651547 None:intergenic 25.0%
! ATCAATCTTCAATGTAAGTA+AGG + chr7.4:13652656-13652675 MS.gene020289:CDS 25.0%
! CACATAATGTTTGTAATAAG+AGG - chr7.4:13652570-13652589 None:intergenic 25.0%
! CTCTTATTACAAACATTATG+TGG + chr7.4:13652568-13652587 MS.gene020289:CDS 25.0%
! GAGAAACTTTATGTATTTGA+AGG + chr7.4:13652753-13652772 MS.gene020289:CDS 25.0%
! GTTGAAACTGAATAAATTTC+TGG - chr7.4:13652362-13652381 None:intergenic 25.0%
! TCAAAAATTGATATCTGTTG+AGG - chr7.4:13650605-13650624 None:intergenic 25.0%
! TGATACAGAAAGATTAACAT+GGG + chr7.4:13652863-13652882 MS.gene020289:CDS 25.0%
! TTAATGAAGAAAAAGCCAAT+AGG + chr7.4:13652534-13652553 MS.gene020289:CDS 25.0%
!! ACAAAATGTAAAAGACCTTT+TGG - chr7.4:13650341-13650360 None:intergenic 25.0%
!! ACTTGTTTCTTGGAATTATT+GGG + chr7.4:13652790-13652809 MS.gene020289:CDS 25.0%
!! ATCAATTTTTGATGTTCTTG+TGG + chr7.4:13650613-13650632 MS.gene020289:CDS 25.0%
!! TCAATTTTTGATGTTCTTGT+GGG + chr7.4:13650614-13650633 MS.gene020289:CDS 25.0%
!! TTCTTCATTAACTCTTTTGT+AGG - chr7.4:13652525-13652544 None:intergenic 25.0%
!! TTCTTCTAGTGTTGTTAATT+CGG + chr7.4:13650966-13650985 MS.gene020289:CDS 25.0%
!! TTCTTTTATACCAAAACCAA+GGG - chr7.4:13650420-13650439 None:intergenic 25.0%
AAAATTGAGAGAACCATTCA+AGG + chr7.4:13651706-13651725 MS.gene020289:CDS 30.0%
AAAGTTGAAGTTGTGAGAAA+TGG + chr7.4:13650575-13650594 MS.gene020289:CDS 30.0%
AACAAAAAAGGAGCTCTTAA+AGG - chr7.4:13650761-13650780 None:intergenic 30.0%
AACAACAGTTCAACTTCTTT+GGG + chr7.4:13652449-13652468 MS.gene020289:CDS 30.0%
AATAATGATCATGCAAATGC+TGG + chr7.4:13652012-13652031 MS.gene020289:CDS 30.0%
AATCTCATTATGGATACACT+AGG + chr7.4:13652474-13652493 MS.gene020289:CDS 30.0%
AATCTTCAATGTAAGTAAGG+AGG + chr7.4:13652659-13652678 MS.gene020289:CDS 30.0%
AGAAGTTGAACTGTTGTTAA+AGG - chr7.4:13652447-13652466 None:intergenic 30.0%
AGTTGAATTCGTTGAAAACT+TGG - chr7.4:13652722-13652741 None:intergenic 30.0%
ATTAGATTTAGGTATGGACA+TGG + chr7.4:13651477-13651496 MS.gene020289:CDS 30.0%
CAATAATTCCAAGAAACAAG+TGG - chr7.4:13652791-13652810 None:intergenic 30.0%
CTGATACAGAAAGATTAACA+TGG + chr7.4:13652862-13652881 MS.gene020289:CDS 30.0%
GTTGAAATTAAGATGATCAC+CGG + chr7.4:13651934-13651953 MS.gene020289:CDS 30.0%
TAACAACAGTTCAACTTCTT+TGG + chr7.4:13652448-13652467 MS.gene020289:CDS 30.0%
TATTGTTCTTCAAATCCTGA+TGG + chr7.4:13652818-13652837 MS.gene020289:CDS 30.0%
TCTCATTTGATTCAATGTCA+AGG - chr7.4:13651266-13651285 None:intergenic 30.0%
TGAATTCAGAAACTATACAG+AGG + chr7.4:13652053-13652072 MS.gene020289:CDS 30.0%
TTACTCCATGAAAAGAATGA+TGG + chr7.4:13651159-13651178 MS.gene020289:CDS 30.0%
TTCCTTCAGTTATACTAAGT+GGG + chr7.4:13652971-13652990 MS.gene020289:CDS 30.0%
TTGTTAATTCGGTATTTCAC+CGG + chr7.4:13650977-13650996 MS.gene020289:CDS 30.0%
! AAATGTAAAAGACCTTTTGG+TGG - chr7.4:13650338-13650357 None:intergenic 30.0%
! ATTCTGTTGCAACTGTTTTA+AGG + chr7.4:13652310-13652329 MS.gene020289:CDS 30.0%
! CACTTGTTTCTTGGAATTAT+TGG + chr7.4:13652789-13652808 MS.gene020289:CDS 30.0%
! CTTAAAAACCACTTGTTTCT+TGG + chr7.4:13652780-13652799 MS.gene020289:CDS 30.0%
! CTTCTGATTATGCAGTTTTA+TGG + chr7.4:13652630-13652649 MS.gene020289:CDS 30.0%
! GATCTGAACCTGAAATTTTA+GGG + chr7.4:13651419-13651438 MS.gene020289:CDS 30.0%
! GTTCTTTTATACCAAAACCA+AGG - chr7.4:13650421-13650440 None:intergenic 30.0%
! TCCTATATCAGCTTCTTTTA+AGG - chr7.4:13652217-13652236 None:intergenic 30.0%
! TGAAAACTTTTCCAATGCAA+TGG + chr7.4:13651321-13651340 MS.gene020289:CDS 30.0%
!! GGTTTTGGTATAAAAGAACA+TGG + chr7.4:13650422-13650441 MS.gene020289:CDS 30.0%
AAAGAAAGGTCATGTAGTTG+CGG + chr7.4:13652161-13652180 MS.gene020289:CDS 35.0%
AAAGATTAACATGGGAGCAA+TGG + chr7.4:13652871-13652890 MS.gene020289:CDS 35.0%
AAAGGGAATAAGGAATTTCG+AGG + chr7.4:13651016-13651035 MS.gene020289:CDS 35.0%
AACCCACTTAGTATAACTGA+AGG - chr7.4:13652976-13652995 None:intergenic 35.0%
AACTCAGAAAAGAAACGAAG+TGG + chr7.4:13651544-13651563 MS.gene020289:CDS 35.0%
AAGATTAACATGGGAGCAAT+GGG + chr7.4:13652872-13652891 MS.gene020289:CDS 35.0%
AAGGCAATAGCAATAGAATG+TGG + chr7.4:13651976-13651995 MS.gene020289:CDS 35.0%
AATGTTTGTAATAAGAGGCG+CGG - chr7.4:13652565-13652584 None:intergenic 35.0%
ACATGATGAGAAAGGGAATA+AGG + chr7.4:13651006-13651025 MS.gene020289:CDS 35.0%
ACCATCAAAAGTTTGTCCAT+AGG - chr7.4:13652129-13652148 None:intergenic 35.0%
ACTTTCAGCTTGTGAAACTA+TGG + chr7.4:13651204-13651223 MS.gene020289:CDS 35.0%
AGCTGATATAGGACTTTCTA+TGG + chr7.4:13652224-13652243 MS.gene020289:CDS 35.0%
ATGTGGGCAGTATTAGATTT+AGG + chr7.4:13651466-13651485 MS.gene020289:CDS 35.0%
CAATGTAAGTAAGGAGGTAA+AGG + chr7.4:13652665-13652684 MS.gene020289:CDS 35.0%
CACTTAGTATAACTGAAGGA+AGG - chr7.4:13652972-13652991 None:intergenic 35.0%
CATCAAAAGTTTGTCCATAG+GGG - chr7.4:13652127-13652146 None:intergenic 35.0%
CATCAAATGCTTAGAGAAGA+TGG + chr7.4:13651826-13651845 MS.gene020289:CDS 35.0%
CCATCAAAAGTTTGTCCATA+GGG - chr7.4:13652128-13652147 None:intergenic 35.0%
CGATAACATATTCACTGCTA+AGG + chr7.4:13651957-13651976 MS.gene020289:CDS 35.0%
CTTCCTTCAGTTATACTAAG+TGG + chr7.4:13652970-13652989 MS.gene020289:CDS 35.0%
CTTGGAACATTTCCAACAAA+GGG + chr7.4:13650242-13650261 MS.gene020289:CDS 35.0%
CTTTCAGCTTGTGAAACTAT+GGG + chr7.4:13651205-13651224 MS.gene020289:CDS 35.0%
GATAAAAATACTCCATCAGC+TGG - chr7.4:13650674-13650693 None:intergenic 35.0%
GCCTTAAAAGAAGCTGATAT+AGG + chr7.4:13652213-13652232 MS.gene020289:CDS 35.0%
GCTGATATAGGACTTTCTAT+GGG + chr7.4:13652225-13652244 MS.gene020289:CDS 35.0%
GGCAGTATTAGATTTAGGTA+TGG + chr7.4:13651471-13651490 MS.gene020289:CDS 35.0%
GTAACTCACATGATGAGAAA+GGG + chr7.4:13650999-13651018 MS.gene020289:CDS 35.0%
GTATAAAAGAACATGGACCA+GGG + chr7.4:13650429-13650448 MS.gene020289:CDS 35.0%
GTGCAATGCTTAAGAAAGAA+AGG + chr7.4:13652147-13652166 MS.gene020289:CDS 35.0%
TATAAAAGAACATGGACCAG+GGG + chr7.4:13650430-13650449 MS.gene020289:CDS 35.0%
TCTTGGAACATTTCCAACAA+AGG + chr7.4:13650241-13650260 MS.gene020289:CDS 35.0%
TCTTTGGGTGAATCTCATTA+TGG + chr7.4:13652464-13652483 MS.gene020289:CDS 35.0%
TGTGGGAAAAAACACATCAT+GGG + chr7.4:13650829-13650848 MS.gene020289:CDS 35.0%
TTAAAGGCTCAATCTCTACA+TGG - chr7.4:13650745-13650764 None:intergenic 35.0%
TTCAAGACCAAGACAAAACT+TGG - chr7.4:13651293-13651312 None:intergenic 35.0%
TTGAAGTCCTTAACTGAGTT+TGG + chr7.4:13650194-13650213 MS.gene020289:CDS 35.0%
TTGAAGTTGTGAGAAATGGA+AGG + chr7.4:13650579-13650598 MS.gene020289:CDS 35.0%
TTTATTGCAGCAGTTTCTTC+TGG + chr7.4:13652414-13652433 MS.gene020289:CDS 35.0%
TTTCCAACAAAGGGAATCAT+TGG + chr7.4:13650251-13650270 MS.gene020289:CDS 35.0%
TTTCTCATCATGTGAGTTAC+CGG - chr7.4:13650999-13651018 None:intergenic 35.0%
! AAGGAAGGTTTATCTGAAAC+AGG - chr7.4:13652957-13652976 None:intergenic 35.0%
! ATATTGATAGTAATGGCGTG+AGG + chr7.4:13651656-13651675 MS.gene020289:CDS 35.0%
! ATCATTCTTCTTGTTTGTGC+TGG + chr7.4:13650389-13650408 MS.gene020289:CDS 35.0%
! ATCTGCCATCATTCTTTTCA+TGG - chr7.4:13651167-13651186 None:intergenic 35.0%
! GGATCTGAACCTGAAATTTT+AGG + chr7.4:13651418-13651437 MS.gene020289:CDS 35.0%
! GTTATCTGCACTGATAAAAC+AGG + chr7.4:13651238-13651257 MS.gene020289:CDS 35.0%
! TCTAGCAGTTTTTCTTGTTG+TGG + chr7.4:13650481-13650500 MS.gene020289:CDS 35.0%
! TGGAACAACTCAAGAACTTT+TGG - chr7.4:13651349-13651368 None:intergenic 35.0%
! TTCTTGAGTTGTTCCAACAA+GGG + chr7.4:13651353-13651372 MS.gene020289:CDS 35.0%
! TTGGAATTGCAGTTGTTTCA+TGG + chr7.4:13652904-13652923 MS.gene020289:CDS 35.0%
!! ATGTATTGCTTTTGCTCACA+CGG + chr7.4:13651750-13651769 MS.gene020289:CDS 35.0%
!! GCTGATGGAGTATTTTTATC+CGG + chr7.4:13650674-13650693 MS.gene020289:CDS 35.0%
!!! ATATCAGCTTCTTTTAAGGC+AGG - chr7.4:13652213-13652232 None:intergenic 35.0%
!!! TGTTGCAACTGTTTTAAGGT+GGG + chr7.4:13652314-13652333 MS.gene020289:CDS 35.0%
AACTCCTCCAAACTCAGTTA+AGG - chr7.4:13650204-13650223 None:intergenic 40.0%
AAGTCCTTAACTGAGTTTGG+AGG + chr7.4:13650197-13650216 MS.gene020289:CDS 40.0%
AATGATGGCAGATCATGCTA+TGG + chr7.4:13651174-13651193 MS.gene020289:CDS 40.0%
ACCAAACACTAAGAAAGCTG+TGG + chr7.4:13651891-13651910 MS.gene020289:CDS 40.0%
AGATTAACATGGGAGCAATG+GGG + chr7.4:13652873-13652892 MS.gene020289:CDS 40.0%
AGCAGTGAATATGTTATCGC+CGG - chr7.4:13651956-13651975 None:intergenic 40.0%
AGCTTTACTAAGCAAGCAAG+TGG - chr7.4:13652930-13652949 None:intergenic 40.0%
ATGAGTATAGCTTTCTCCGT+TGG - chr7.4:13651448-13651467 None:intergenic 40.0%
CAGATGCTTGTAACATCTGT+GGG + chr7.4:13650812-13650831 MS.gene020289:CDS 40.0%
CGAATTCAACTCAAGAAGCA+TGG + chr7.4:13652731-13652750 MS.gene020289:CDS 40.0%
CTATACAGAGGAAGAGAGAA+TGG + chr7.4:13652065-13652084 MS.gene020289:CDS 40.0%
CTGTGGGAAAAAACACATCA+TGG + chr7.4:13650828-13650847 MS.gene020289:CDS 40.0%
CTTCCAATGATTCCCTTTGT+TGG - chr7.4:13650257-13650276 None:intergenic 40.0%
GACAAGTTAACCTCTTCGAT+TGG + chr7.4:13650914-13650933 MS.gene020289:CDS 40.0%
GACGATATCGGAACTTTCTT+TGG - chr7.4:13652271-13652290 None:intergenic 40.0%
GAGCAATGGGGAATTTGTAT+TGG + chr7.4:13652885-13652904 MS.gene020289:CDS 40.0%
GATATGAAGCTTGAGCTAGA+AGG - chr7.4:13652599-13652618 None:intergenic 40.0%
GATGTTGTGTCTCTCAAGAT+TGG + chr7.4:13650638-13650657 MS.gene020289:CDS 40.0%
GGAGAAAGCTATACTCATGT+GGG + chr7.4:13651450-13651469 MS.gene020289:CDS 40.0%
GGTAACTCACATGATGAGAA+AGG + chr7.4:13650998-13651017 MS.gene020289:CDS 40.0%
GGTATAAAAGAACATGGACC+AGG + chr7.4:13650428-13650447 MS.gene020289:CDS 40.0%
GTGCAGATGATATTTCGAGA+CGG + chr7.4:13650276-13650295 MS.gene020289:CDS 40.0%
GTGGGAAAAAACACATCATG+GGG + chr7.4:13650830-13650849 MS.gene020289:CDS 40.0%
GTTAACCTCTTCGATTGGAA+AGG + chr7.4:13650919-13650938 MS.gene020289:CDS 40.0%
TACAAAAAGCCACCACCAAA+AGG + chr7.4:13650323-13650342 MS.gene020289:CDS 40.0%
TATGGATACACTAGGTGCTT+TGG + chr7.4:13652482-13652501 MS.gene020289:CDS 40.0%
TATTGAGTCCTATCCCTTGT+TGG - chr7.4:13651369-13651388 None:intergenic 40.0%
TCAGATGCTTGTAACATCTG+TGG + chr7.4:13650811-13650830 MS.gene020289:CDS 40.0%
TCCACAGCTTTCTTAGTGTT+TGG - chr7.4:13651895-13651914 None:intergenic 40.0%
TGAGAGAACCATTCAAGGTA+TGG + chr7.4:13651711-13651730 MS.gene020289:CDS 40.0%
TGAGTATAGCTTTCTCCGTT+GGG - chr7.4:13651447-13651466 None:intergenic 40.0%
TGTTCTTCAAATCCTGATGG+TGG + chr7.4:13652821-13652840 MS.gene020289:CDS 40.0%
TTCTTAGTGTTTGGTCTGCA+TGG - chr7.4:13651886-13651905 None:intergenic 40.0%
TTGACATTGCTTGGTATCGT+TGG + chr7.4:13651850-13651869 MS.gene020289:CDS 40.0%
! CCCTATGGACAAACTTTTGA+TGG + chr7.4:13652125-13652144 MS.gene020289:CDS 40.0%
! GTCGTCTAAGATGACGATAT+CGG - chr7.4:13652283-13652302 None:intergenic 40.0%
! GTTCTTGAGTTGTTCCAACA+AGG + chr7.4:13651352-13651371 MS.gene020289:CDS 40.0%
! TGTAAAAGACCTTTTGGTGG+TGG - chr7.4:13650335-13650354 None:intergenic 40.0%
! TTCTGTTGTGAGTATCGTTG+CGG + chr7.4:13651069-13651088 MS.gene020289:CDS 40.0%
!! AACGAAGTGGTGTTGCTATA+AGG + chr7.4:13651557-13651576 MS.gene020289:CDS 40.0%
!! AGAGTGACCAAGTTTTGTCT+TGG + chr7.4:13651283-13651302 MS.gene020289:CDS 40.0%
!! ATTTCAGGTTCAGATCCTGA+TGG - chr7.4:13651415-13651434 None:intergenic 40.0%
!! GAAGATGGATTGACATTGCT+TGG + chr7.4:13651841-13651860 MS.gene020289:CDS 40.0%
!! TGAAATTTTAGGGAGCCCAA+CGG + chr7.4:13651429-13651448 MS.gene020289:CDS 40.0%
!!! AACTTTTGGTGCCATTGCAT+TGG - chr7.4:13651335-13651354 None:intergenic 40.0%
!!! AGAGCTCCTTTTTTGTTGTC+CGG + chr7.4:13650764-13650783 MS.gene020289:CDS 40.0%
!!! CTGTTGCAACTGTTTTAAGG+TGG + chr7.4:13652313-13652332 MS.gene020289:CDS 40.0%
!!! GTTGCAACTGTTTTAAGGTG+GGG + chr7.4:13652315-13652334 MS.gene020289:CDS 40.0%
AATGCTGGAGAAGTGGTTGA+AGG + chr7.4:13652027-13652046 MS.gene020289:CDS 45.0%
ACCTCTTCGATTGGAAAGGT+TGG + chr7.4:13650923-13650942 MS.gene020289:CDS 45.0%
ACTAGCAGCCATACCTTGAA+TGG - chr7.4:13651722-13651741 None:intergenic 45.0%
ACTCCGTTCTTCTTCGTCAA+GGG - chr7.4:13651686-13651705 None:intergenic 45.0%
ACTGAGTTTGGAGGAGTTGA+AGG + chr7.4:13650206-13650225 MS.gene020289:CDS 45.0%
AGTCCCTTGACGAAGAAGAA+CGG + chr7.4:13651680-13651699 MS.gene020289:CDS 45.0%
AGTCGATAAGATCCGAGTGA+TGG + chr7.4:13652092-13652111 MS.gene020289:CDS 45.0%
AGTGTCTATAAGCCTCCATC+AGG + chr7.4:13651397-13651416 MS.gene020289:CDS 45.0%
ATCCGGTTATTCGTTGCAAG+TGG + chr7.4:13650691-13650710 MS.gene020289:CDS 45.0%
CGGAGAAAGCTATACTCATG+TGG + chr7.4:13651449-13651468 MS.gene020289:CDS 45.0%
CTTTGGCACTAGCAACAGAA+AGG + chr7.4:13652499-13652518 MS.gene020289:CDS 45.0%
GAGTTGTTCCAACAAGGGAT+AGG + chr7.4:13651358-13651377 MS.gene020289:CDS 45.0%
GGAACTTTCTTTGGCGACTT+CGG - chr7.4:13652262-13652281 None:intergenic 45.0%
GGACTTTCTATGGGAATCCA+AGG + chr7.4:13652234-13652253 MS.gene020289:CDS 45.0%
GGGATAGGACTCAATACAAC+TGG + chr7.4:13651373-13651392 MS.gene020289:CDS 45.0%
GGTGATCAAATTCCAGCTGA+TGG + chr7.4:13650659-13650678 MS.gene020289:CDS 45.0%
GTCCACTTGCAACGAATAAC+CGG - chr7.4:13650696-13650715 None:intergenic 45.0%
GTTGGGCTCCCTAAAATTTC+AGG - chr7.4:13651430-13651449 None:intergenic 45.0%
TACTCCGTTCTTCTTCGTCA+AGG - chr7.4:13651687-13651706 None:intergenic 45.0%
TCATGCAAATGCTGGAGAAG+TGG + chr7.4:13652020-13652039 MS.gene020289:CDS 45.0%
TCCAACCTTTCCAATCGAAG+AGG - chr7.4:13650927-13650946 None:intergenic 45.0%
TTTGCGCCGGACAACAAAAA+AGG - chr7.4:13650773-13650792 None:intergenic 45.0%
! TTTGGAGGAGTTGAAGGTGT+TGG + chr7.4:13650212-13650231 MS.gene020289:CDS 45.0%
!! GAAGGTGTTGGACATGTTCT+TGG + chr7.4:13650224-13650243 MS.gene020289:CDS 45.0%
!! TCGAAGAGGTTAACTTGTCG+AGG - chr7.4:13650913-13650932 None:intergenic 45.0%
AAAGAACATGGACCAGGGGA+AGG + chr7.4:13650434-13650453 MS.gene020289:CDS 50.0%
ACAACAGCGTTCACGTGCAT+TGG + chr7.4:13651593-13651612 MS.gene020289:CDS 50.0%
AGAGTGAGTGTCACAGCCAA+TGG - chr7.4:13651136-13651155 None:intergenic 50.0%
AGCGTTCACGTGCATTGGAA+AGG + chr7.4:13651598-13651617 MS.gene020289:CDS 50.0%
GATGGCAAGATCATCCCCTA+TGG + chr7.4:13652110-13652129 MS.gene020289:CDS 50.0%
GGGATGATCTTGCCATCACT+CGG - chr7.4:13652107-13652126 None:intergenic 50.0%
GTGGAAACTTGCAAAGCTGC+AGG + chr7.4:13651910-13651929 MS.gene020289:CDS 50.0%
GTGGACGAATCTAGCATGAC+AGG + chr7.4:13650710-13650729 MS.gene020289:CDS 50.0%
TCAGGTTCAGATCCTGATGG+AGG - chr7.4:13651412-13651431 None:intergenic 50.0%
TCCTTAGCAGTTCCACCATC+AGG - chr7.4:13652836-13652855 None:intergenic 50.0%
TGGCAGATCATGCTATGGTG+AGG + chr7.4:13651179-13651198 MS.gene020289:CDS 50.0%
TTGGTCTGCATGGATCCTTG+AGG - chr7.4:13651876-13651895 None:intergenic 50.0%
TTGTCGAGGCGTGCTTGTAA+CGG - chr7.4:13650899-13650918 None:intergenic 50.0%
! TCCATCGACAACTTTTGCGC+CGG - chr7.4:13650786-13650805 None:intergenic 50.0%
!! GTTTGTGCTGGTCTTTCCCT+TGG + chr7.4:13650401-13650420 MS.gene020289:CDS 50.0%
!! TCCTGATGGTGGAACTGCTA+AGG + chr7.4:13652832-13652851 MS.gene020289:CDS 50.0%
!!! GCTGGTCTTTCCCTTGGTTT+TGG + chr7.4:13650407-13650426 MS.gene020289:CDS 50.0%
AACATGGACCAGGGGAAGGT+TGG + chr7.4:13650438-13650457 MS.gene020289:CDS 55.0%
ACAGCCAATGGCAAACCCTC+AGG - chr7.4:13651124-13651143 None:intergenic 55.0%
CAATGGCAAACCCTCAGGGA+TGG - chr7.4:13651119-13651138 None:intergenic 55.0%
CAGCCAATGGCAAACCCTCA+GGG - chr7.4:13651123-13651142 None:intergenic 55.0%
CATCCCTGAGGGTTTGCCAT+TGG + chr7.4:13651117-13651136 MS.gene020289:CDS 55.0%
CCTTCATACCAACCTTCCCC+TGG - chr7.4:13650449-13650468 None:intergenic 55.0%
CTTTGGCGACTTCGGTTCCT+TGG - chr7.4:13652254-13652273 None:intergenic 55.0%
GGTCATGTAGTTGCGGTCAC+AGG + chr7.4:13652168-13652187 MS.gene020289:CDS 55.0%
TCCGGCGCAAAAGTTGTCGA+TGG + chr7.4:13650782-13650801 MS.gene020289:CDS 55.0%
TGCGGCTGCAGTCACTATTG+TGG + chr7.4:13651087-13651106 MS.gene020289:CDS 55.0%
! GCTTGGTATCGTTGGCCTCA+AGG + chr7.4:13651858-13651877 MS.gene020289:CDS 55.0%
! TCGAGACGGCTCGAGTTGTT+CGG + chr7.4:13650290-13650309 MS.gene020289:CDS 55.0%
!! CCAGGGGAAGGTTGGTATGA+AGG + chr7.4:13650446-13650465 MS.gene020289:CDS 55.0%
!! GGGGAAGGTTGGTATGAAGG+TGG + chr7.4:13650449-13650468 MS.gene020289:CDS 55.0%
GTAGTTGCGGTCACAGGCGA+CGG + chr7.4:13652174-13652193 MS.gene020289:CDS 60.0%
GTGGTCGTTGCCATCCCTGA+GGG + chr7.4:13651106-13651125 MS.gene020289:CDS 60.0%
TGTGGTCGTTGCCATCCCTG+AGG + chr7.4:13651105-13651124 MS.gene020289:CDS 60.0%
! AGAGGCGCGGTTCGACCTAT+TGG - chr7.4:13652552-13652571 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 13650185 13653031 13650185 ID=MS.gene020289
chr7.4 mRNA 13650185 13653031 13650185 ID=MS.gene020289.t1;Parent=MS.gene020289
chr7.4 exon 13650185 13653031 13650185 ID=MS.gene020289.t1.exon1;Parent=MS.gene020289.t1
chr7.4 CDS 13650185 13653031 13650185 ID=cds.MS.gene020289.t1;Parent=MS.gene020289.t1
Gene Sequence

>MS.gene020289

GACAAAAACTTGAAGTCCTTAACTGAGTTTGGAGGAGTTGAAGGTGTTGGACATGTTCTTGGAACATTTCCAACAAAGGGAATCATTGGAAGTGCAGATGATATTTCGAGACGGCTCGAGTTGTTCGGTTCAAATACTTACAAAAAGCCACCACCAAAAGGTCTTTTACATTTTGTGCTTGAAGCTTTCAATGATACAACAATTATCATTCTTCTTGTTTGTGCTGGTCTTTCCCTTGGTTTTGGTATAAAAGAACATGGACCAGGGGAAGGTTGGTATGAAGGTGGAAGTATATTTCTAGCAGTTTTTCTTGTTGTGGTTGTAAGTGCACTAAGCAATTTTAGACAAGAAAGACAGTTTCACAAATTGTCTAAGATAAGCAACAATATCAAAGTTGAAGTTGTGAGAAATGGAAGGCCTCAACAGATATCAATTTTTGATGTTCTTGTGGGAGATGTTGTGTCTCTCAAGATTGGTGATCAAATTCCAGCTGATGGAGTATTTTTATCCGGTTATTCGTTGCAAGTGGACGAATCTAGCATGACAGGTGAGAGCGACCATGTAGAGATTGAGCCTTTAAGAGCTCCTTTTTTGTTGTCCGGCGCAAAAGTTGTCGATGGATATGCTCAGATGCTTGTAACATCTGTGGGAAAAAACACATCATGGGGACAAATGATGAGCTCGATATCGCGAGACACTAATGAAAGAACGCCGTTACAAGCACGCCTCGACAAGTTAACCTCTTCGATTGGAAAGGTTGGACTTGCAGTAGCGTTTCTTGTTCTTCTAGTGTTGTTAATTCGGTATTTCACCGGTAACTCACATGATGAGAAAGGGAATAAGGAATTTCGAGGAAGCAAAACCGATATAAACGATGTTATGAATTCTGTTGTGAGTATCGTTGCGGCTGCAGTCACTATTGTGGTCGTTGCCATCCCTGAGGGTTTGCCATTGGCTGTGACACTCACTCTTGCTTACTCCATGAAAAGAATGATGGCAGATCATGCTATGGTGAGGAAACTTTCAGCTTGTGAAACTATGGGATCAGCAACAGTTATCTGCACTGATAAAACAGGTACCTTGACATTGAATCAAATGAGAGTGACCAAGTTTTGTCTTGGTCTTGAAAATATTATTGAAAACTTTTCCAATGCAATGGCACCAAAAGTTCTTGAGTTGTTCCAACAAGGGATAGGACTCAATACAACTGGTAGTGTCTATAAGCCTCCATCAGGATCTGAACCTGAAATTTTAGGGAGCCCAACGGAGAAAGCTATACTCATGTGGGCAGTATTAGATTTAGGTATGGACATGGATGAAATGAAACAGAAGCATAAAGTCCTTCATGTTGAAACATTTAACTCAGAAAAGAAACGAAGTGGTGTTGCTATAAGGAAAGAGAACGACGACAACAGCGTTCACGTGCATTGGAAAGGAGCTGCAGAGATGATACTTGCAATGTGTACAAACTATATTGATAGTAATGGCGTGAGGAAGTCCCTTGACGAAGAAGAACGGAGTAAAATTGAGAGAACCATTCAAGGTATGGCTGCTAGTAGTTTAAGATGTATTGCTTTTGCTCACACGGAAATTTCAGATTCCGAAGATATTGATTATATGATCAAAAGGGAGAAAAAATCACATCAAATGCTTAGAGAAGATGGATTGACATTGCTTGGTATCGTTGGCCTCAAGGATCCATGCAGACCAAACACTAAGAAAGCTGTGGAAACTTGCAAAGCTGCAGGAGTTGAAATTAAGATGATCACCGGCGATAACATATTCACTGCTAAGGCAATAGCAATAGAATGTGGAATATTAGATTCTAATAATGATCATGCAAATGCTGGAGAAGTGGTTGAAGGTGTTGAATTCAGAAACTATACAGAGGAAGAGAGAATGGAAAAAGTCGATAAGATCCGAGTGATGGCAAGATCATCCCCTATGGACAAACTTTTGATGGTGCAATGCTTAAGAAAGAAAGGTCATGTAGTTGCGGTCACAGGCGACGGCACAAACGATGCACCTGCCTTAAAAGAAGCTGATATAGGACTTTCTATGGGAATCCAAGGAACCGAAGTCGCCAAAGAAAGTTCCGATATCGTCATCTTAGACGACAACTTCAATTCTGTTGCAACTGTTTTAAGGTGGGGAAGATGTGTTTACAACAACATCCAGAAATTTATTCAGTTTCAACTAACAGTGAATGTTGCAGCACTTGTGATAAACTTTATTGCAGCAGTTTCTTCTGGTGATGTTCCTTTAACAACAGTTCAACTTCTTTGGGTGAATCTCATTATGGATACACTAGGTGCTTTGGCACTAGCAACAGAAAGGCCTACAAAAGAGTTAATGAAGAAAAAGCCAATAGGTCGAACCGCGCCTCTTATTACAAACATTATGTGGAGAAACCTTCTAGCTCAAGCTTCATATCAGATTGCAGTTCTTCTGATTATGCAGTTTTATGGAAAATCAATCTTCAATGTAAGTAAGGAGGTAAAGGATACACTAATTTTTAATACTTTTGTTCTCTGCCAAGTTTTCAACGAATTCAACTCAAGAAGCATGGAGAAACTTTATGTATTTGAAGGGATTCTTAAAAACCACTTGTTTCTTGGAATTATTGGGATTACTATTGTTCTTCAAATCCTGATGGTGGAACTGCTAAGGAAGTTTGCTGATACAGAAAGATTAACATGGGAGCAATGGGGAATTTGTATTGGAATTGCAGTTGTTTCATGGCCACTTGCTTGCTTAGTAAAGCTCATACCTGTTTCAGATAAACCTTCCTTCAGTTATACTAAGTGGGTTAAATTATTAGTCTACAAGATTAAGAATGCTTTTTAA
Protein sequence

>MS.gene020289.t1

DKNLKSLTEFGGVEGVGHVLGTFPTKGIIGSADDISRRLELFGSNTYKKPPPKGLLHFVLEAFNDTTIIILLVCAGLSLGFGIKEHGPGEGWYEGGSIFLAVFLVVVVSALSNFRQERQFHKLSKISNNIKVEVVRNGRPQQISIFDVLVGDVVSLKIGDQIPADGVFLSGYSLQVDESSMTGESDHVEIEPLRAPFLLSGAKVVDGYAQMLVTSVGKNTSWGQMMSSISRDTNERTPLQARLDKLTSSIGKVGLAVAFLVLLVLLIRYFTGNSHDEKGNKEFRGSKTDINDVMNSVVSIVAAAVTIVVVAIPEGLPLAVTLTLAYSMKRMMADHAMVRKLSACETMGSATVICTDKTGTLTLNQMRVTKFCLGLENIIENFSNAMAPKVLELFQQGIGLNTTGSVYKPPSGSEPEILGSPTEKAILMWAVLDLGMDMDEMKQKHKVLHVETFNSEKKRSGVAIRKENDDNSVHVHWKGAAEMILAMCTNYIDSNGVRKSLDEEERSKIERTIQGMAASSLRCIAFAHTEISDSEDIDYMIKREKKSHQMLREDGLTLLGIVGLKDPCRPNTKKAVETCKAAGVEIKMITGDNIFTAKAIAIECGILDSNNDHANAGEVVEGVEFRNYTEEERMEKVDKIRVMARSSPMDKLLMVQCLRKKGHVVAVTGDGTNDAPALKEADIGLSMGIQGTEVAKESSDIVILDDNFNSVATVLRWGRCVYNNIQKFIQFQLTVNVAALVINFIAAVSSGDVPLTTVQLLWVNLIMDTLGALALATERPTKELMKKKPIGRTAPLITNIMWRNLLAQASYQIAVLLIMQFYGKSIFNVSKEVKDTLIFNTFVLCQVFNEFNSRSMEKLYVFEGILKNHLFLGIIGITIVLQILMVELLRKFADTERLTWEQWGICIGIAVVSWPLACLVKLIPVSDKPSFSYTKWVKLLVYKIKNAF