Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022324.t1 | XP_003601227.1 | 97.3 | 329 | 9 | 0 | 1 | 329 | 1 | 329 | 1.50E-174 | 622.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022324.t1 | Q6RVV4 | 44.2 | 312 | 170 | 2 | 13 | 324 | 9 | 316 | 1.8e-67 | 257.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022324.t1 | G7J4W0 | 97.3 | 329 | 9 | 0 | 1 | 329 | 1 | 329 | 1.1e-174 | 622.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene006265 | MS.gene022324 | PPI |
| MS.gene34138 | MS.gene022324 | PPI |
| MS.gene34140 | MS.gene022324 | PPI |
| MS.gene006263 | MS.gene022324 | PPI |
| MS.gene061155 | MS.gene022324 | PPI |
| MS.gene49118 | MS.gene022324 | PPI |
| MS.gene022324 | MS.gene49117 | PPI |
| MS.gene022324 | MS.gene061158 | PPI |
| MS.gene34141 | MS.gene022324 | PPI |
| MS.gene022324 | MS.gene061157 | PPI |
| MS.gene006264 | MS.gene022324 | PPI |
| MS.gene49120 | MS.gene022324 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022324.t1 | MTR_3g077410 | 97.264 | 329 | 9 | 0 | 1 | 329 | 1 | 329 | 0.0 | 663 |
| MS.gene022324.t1 | MTR_4g105310 | 83.030 | 330 | 56 | 0 | 1 | 330 | 12 | 341 | 0.0 | 577 |
| MS.gene022324.t1 | MTR_4g105310 | 82.283 | 254 | 45 | 0 | 1 | 254 | 12 | 265 | 2.08e-157 | 441 |
| MS.gene022324.t1 | MTR_8g099995 | 52.923 | 325 | 149 | 2 | 1 | 323 | 1 | 323 | 9.84e-123 | 356 |
| MS.gene022324.t1 | MTR_7g099850 | 49.536 | 323 | 158 | 3 | 1 | 322 | 1 | 319 | 1.12e-104 | 310 |
| MS.gene022324.t1 | MTR_1g068970 | 47.826 | 322 | 164 | 2 | 1 | 322 | 1 | 318 | 9.50e-103 | 305 |
| MS.gene022324.t1 | MTR_8g098415 | 44.805 | 308 | 166 | 2 | 7 | 314 | 2 | 305 | 6.41e-93 | 279 |
| MS.gene022324.t1 | MTR_8g098415 | 44.805 | 308 | 166 | 2 | 7 | 314 | 2 | 305 | 7.80e-93 | 279 |
| MS.gene022324.t1 | MTR_1g100395 | 47.115 | 312 | 161 | 2 | 13 | 324 | 8 | 315 | 3.65e-92 | 277 |
| MS.gene022324.t1 | MTR_6g053550 | 46.774 | 310 | 160 | 2 | 14 | 321 | 17 | 323 | 4.72e-90 | 272 |
| MS.gene022324.t1 | MTR_1g100305 | 43.910 | 312 | 171 | 2 | 13 | 324 | 8 | 315 | 4.26e-89 | 270 |
| MS.gene022324.t1 | MTR_1g100335 | 44.231 | 312 | 170 | 2 | 13 | 324 | 8 | 315 | 1.06e-85 | 261 |
| MS.gene022324.t1 | MTR_1g100315 | 43.269 | 312 | 173 | 2 | 13 | 324 | 8 | 315 | 3.30e-84 | 257 |
| MS.gene022324.t1 | MTR_1g068970 | 44.841 | 252 | 138 | 1 | 71 | 322 | 1 | 251 | 1.18e-73 | 228 |
| MS.gene022324.t1 | MTR_6g053600 | 46.415 | 265 | 137 | 3 | 15 | 277 | 4 | 265 | 4.31e-72 | 225 |
| MS.gene022324.t1 | MTR_8g098415 | 43.373 | 249 | 140 | 1 | 66 | 314 | 1 | 248 | 1.92e-71 | 222 |
| MS.gene022324.t1 | MTR_1g100335 | 45.968 | 248 | 131 | 1 | 13 | 260 | 8 | 252 | 1.63e-67 | 213 |
| MS.gene022324.t1 | MTR_1g100335 | 45.161 | 248 | 132 | 2 | 13 | 260 | 8 | 251 | 4.26e-67 | 211 |
| MS.gene022324.t1 | MTR_8g098415 | 46.575 | 219 | 114 | 1 | 7 | 225 | 2 | 217 | 1.53e-63 | 201 |
| MS.gene022324.t1 | MTR_8g022500 | 63.964 | 111 | 15 | 1 | 42 | 152 | 431 | 516 | 2.49e-33 | 129 |
| MS.gene022324.t1 | MTR_3g091070 | 31.579 | 304 | 167 | 11 | 35 | 321 | 38 | 317 | 3.71e-29 | 114 |
| MS.gene022324.t1 | MTR_6g041980 | 29.924 | 264 | 168 | 7 | 37 | 298 | 66 | 314 | 5.80e-29 | 114 |
| MS.gene022324.t1 | MTR_3g009190 | 29.642 | 307 | 193 | 8 | 32 | 321 | 57 | 357 | 3.83e-27 | 109 |
| MS.gene022324.t1 | MTR_3g009190 | 29.642 | 307 | 193 | 8 | 32 | 321 | 75 | 375 | 4.33e-27 | 110 |
| MS.gene022324.t1 | MTR_6g453040 | 29.316 | 307 | 194 | 8 | 32 | 321 | 35 | 335 | 9.01e-27 | 108 |
| MS.gene022324.t1 | MTR_2g079710 | 27.937 | 315 | 189 | 11 | 39 | 321 | 88 | 396 | 4.60e-25 | 104 |
| MS.gene022324.t1 | MTR_6g081020 | 29.773 | 309 | 179 | 10 | 38 | 321 | 62 | 357 | 2.42e-24 | 102 |
| MS.gene022324.t1 | MTR_3g009190 | 29.252 | 294 | 185 | 8 | 45 | 321 | 69 | 356 | 4.40e-24 | 101 |
| MS.gene022324.t1 | MTR_6g041980 | 29.082 | 196 | 129 | 4 | 37 | 230 | 66 | 253 | 2.10e-20 | 89.4 |
| MS.gene022324.t1 | MTR_4g087460 | 28.667 | 300 | 166 | 8 | 40 | 305 | 24 | 309 | 3.95e-16 | 78.2 |
| MS.gene022324.t1 | MTR_6g453040 | 30.198 | 202 | 126 | 4 | 34 | 224 | 59 | 256 | 8.87e-16 | 77.0 |
| MS.gene022324.t1 | MTR_1g051165 | 28.102 | 274 | 157 | 10 | 39 | 295 | 18 | 268 | 1.43e-14 | 73.2 |
| MS.gene022324.t1 | MTR_4g058840 | 27.189 | 217 | 135 | 5 | 36 | 248 | 12 | 209 | 2.90e-14 | 72.4 |
| MS.gene022324.t1 | MTR_4g058820 | 27.189 | 217 | 135 | 5 | 36 | 248 | 12 | 209 | 2.90e-14 | 72.4 |
| MS.gene022324.t1 | MTR_4g058810 | 27.650 | 217 | 134 | 7 | 36 | 248 | 12 | 209 | 8.32e-14 | 70.9 |
| MS.gene022324.t1 | MTR_7g076660 | 27.055 | 292 | 152 | 10 | 40 | 292 | 9 | 278 | 1.54e-13 | 70.5 |
| MS.gene022324.t1 | MTR_7g076660 | 27.055 | 292 | 152 | 10 | 40 | 292 | 10 | 279 | 1.78e-13 | 70.1 |
| MS.gene022324.t1 | MTR_6g453040 | 29.474 | 190 | 119 | 4 | 32 | 210 | 57 | 242 | 8.23e-13 | 67.8 |
| MS.gene022324.t1 | MTR_8g031610 | 24.839 | 310 | 163 | 10 | 38 | 299 | 5 | 292 | 7.64e-12 | 65.5 |
| MS.gene022324.t1 | MTR_7g076655 | 25.597 | 293 | 155 | 9 | 40 | 292 | 9 | 278 | 1.95e-11 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene022324.t1 | AT5G50130 | 70.181 | 332 | 95 | 3 | 1 | 330 | 1 | 330 | 7.95e-167 | 468 |
| MS.gene022324.t1 | AT5G50130 | 68.966 | 290 | 90 | 0 | 41 | 330 | 7 | 296 | 1.41e-146 | 415 |
| MS.gene022324.t1 | AT5G50130 | 68.729 | 291 | 90 | 1 | 41 | 330 | 7 | 297 | 2.48e-145 | 412 |
| MS.gene022324.t1 | AT5G50130 | 70.968 | 279 | 77 | 3 | 1 | 277 | 1 | 277 | 1.90e-139 | 398 |
| MS.gene022324.t1 | AT5G50130 | 70.714 | 280 | 77 | 4 | 1 | 277 | 1 | 278 | 5.95e-138 | 394 |
| MS.gene022324.t1 | AT5G50130 | 70.543 | 258 | 73 | 2 | 1 | 257 | 1 | 256 | 1.33e-129 | 370 |
| MS.gene022324.t1 | AT5G50130 | 70.270 | 259 | 73 | 3 | 1 | 257 | 1 | 257 | 4.14e-128 | 367 |
| MS.gene022324.t1 | AT5G50130 | 69.328 | 238 | 72 | 1 | 41 | 277 | 7 | 244 | 5.82e-118 | 342 |
| MS.gene022324.t1 | AT4G24050 | 52.012 | 323 | 150 | 2 | 1 | 321 | 1 | 320 | 1.67e-117 | 342 |
| MS.gene022324.t1 | AT5G50130 | 69.038 | 239 | 72 | 2 | 41 | 277 | 7 | 245 | 2.19e-116 | 338 |
| MS.gene022324.t1 | AT1G64590 | 54.799 | 323 | 141 | 4 | 1 | 321 | 1 | 320 | 1.24e-105 | 312 |
| MS.gene022324.t1 | AT4G24050 | 51.254 | 279 | 129 | 3 | 1 | 277 | 1 | 274 | 7.37e-96 | 285 |
| MS.gene022324.t1 | AT5G02540 | 50.476 | 315 | 152 | 2 | 8 | 322 | 7 | 317 | 1.74e-95 | 286 |
| MS.gene022324.t1 | AT2G37540 | 47.134 | 314 | 162 | 2 | 8 | 321 | 7 | 316 | 3.29e-95 | 285 |
| MS.gene022324.t1 | AT4G23420 | 44.984 | 309 | 167 | 1 | 7 | 315 | 2 | 307 | 2.50e-92 | 278 |
| MS.gene022324.t1 | AT4G23420 | 44.984 | 309 | 167 | 1 | 7 | 315 | 2 | 307 | 2.50e-92 | 278 |
| MS.gene022324.t1 | AT4G23420 | 45.515 | 301 | 161 | 1 | 15 | 315 | 27 | 324 | 6.15e-91 | 275 |
| MS.gene022324.t1 | AT4G23430 | 44.337 | 309 | 169 | 1 | 7 | 315 | 2 | 307 | 2.29e-90 | 273 |
| MS.gene022324.t1 | AT4G23430 | 44.660 | 309 | 166 | 3 | 7 | 315 | 2 | 305 | 4.21e-88 | 267 |
| MS.gene022324.t1 | AT4G11410 | 45.364 | 302 | 161 | 2 | 13 | 314 | 8 | 305 | 1.79e-87 | 265 |
| MS.gene022324.t1 | AT4G23430 | 41.748 | 309 | 167 | 2 | 7 | 315 | 2 | 297 | 2.24e-80 | 247 |
| MS.gene022324.t1 | AT4G23430 | 42.071 | 309 | 164 | 4 | 7 | 315 | 2 | 295 | 4.36e-78 | 241 |
| MS.gene022324.t1 | AT2G37540 | 48.750 | 240 | 120 | 1 | 8 | 247 | 7 | 243 | 7.20e-76 | 234 |
| MS.gene022324.t1 | AT4G23420 | 43.939 | 264 | 145 | 1 | 7 | 270 | 2 | 262 | 5.91e-73 | 226 |
| MS.gene022324.t1 | AT4G11410 | 45.935 | 246 | 130 | 1 | 13 | 258 | 8 | 250 | 1.31e-71 | 223 |
| MS.gene022324.t1 | AT4G23420 | 46.862 | 239 | 124 | 1 | 7 | 245 | 2 | 237 | 1.92e-71 | 223 |
| MS.gene022324.t1 | AT4G09750 | 29.368 | 269 | 163 | 7 | 37 | 298 | 43 | 291 | 6.99e-29 | 114 |
| MS.gene022324.t1 | AT4G27760 | 32.154 | 311 | 177 | 9 | 34 | 321 | 52 | 351 | 1.85e-28 | 113 |
| MS.gene022324.t1 | AT5G04070 | 30.945 | 307 | 180 | 11 | 41 | 327 | 62 | 356 | 1.78e-27 | 110 |
| MS.gene022324.t1 | AT5G53090 | 32.154 | 311 | 177 | 10 | 34 | 321 | 51 | 350 | 2.56e-27 | 110 |
| MS.gene022324.t1 | AT5G54190 | 26.837 | 313 | 191 | 11 | 41 | 321 | 96 | 402 | 1.20e-26 | 109 |
| MS.gene022324.t1 | AT4G27440 | 28.115 | 313 | 187 | 11 | 41 | 321 | 92 | 398 | 5.96e-26 | 107 |
| MS.gene022324.t1 | AT4G27440 | 28.115 | 313 | 187 | 11 | 41 | 321 | 92 | 398 | 5.96e-26 | 107 |
| MS.gene022324.t1 | AT5G15940 | 29.801 | 302 | 183 | 10 | 41 | 322 | 63 | 355 | 9.59e-26 | 105 |
| MS.gene022324.t1 | AT5G15940 | 29.801 | 302 | 183 | 10 | 41 | 322 | 64 | 356 | 1.01e-25 | 105 |
| MS.gene022324.t1 | AT5G15940 | 29.801 | 302 | 183 | 10 | 41 | 322 | 64 | 356 | 1.01e-25 | 105 |
| MS.gene022324.t1 | AT1G03630 | 29.412 | 323 | 173 | 13 | 39 | 321 | 89 | 396 | 4.58e-25 | 104 |
| MS.gene022324.t1 | AT1G03630 | 29.412 | 323 | 173 | 13 | 39 | 321 | 91 | 398 | 4.73e-25 | 104 |
| MS.gene022324.t1 | AT5G53100 | 30.519 | 308 | 179 | 9 | 38 | 321 | 45 | 341 | 1.44e-24 | 102 |
| MS.gene022324.t1 | AT5G15940 | 30.502 | 259 | 155 | 7 | 41 | 281 | 64 | 315 | 5.73e-23 | 97.4 |
| MS.gene022324.t1 | AT2G24190 | 25.325 | 308 | 157 | 13 | 36 | 292 | 8 | 293 | 9.56e-14 | 71.2 |
| MS.gene022324.t1 | AT2G24190 | 24.834 | 302 | 156 | 12 | 40 | 292 | 9 | 288 | 1.06e-13 | 70.9 |
| MS.gene022324.t1 | AT5G61830 | 23.549 | 293 | 171 | 7 | 40 | 297 | 39 | 313 | 3.59e-13 | 69.7 |
| MS.gene022324.t1 | AT5G61830 | 23.549 | 293 | 171 | 7 | 40 | 297 | 39 | 313 | 3.59e-13 | 69.7 |
| MS.gene022324.t1 | AT2G17845 | 29.577 | 213 | 126 | 7 | 41 | 247 | 53 | 247 | 1.17e-12 | 68.2 |
| MS.gene022324.t1 | AT1G63380 | 29.358 | 218 | 118 | 8 | 39 | 245 | 26 | 218 | 7.20e-12 | 65.5 |
| MS.gene022324.t1 | AT1G63380 | 29.091 | 220 | 120 | 8 | 39 | 247 | 21 | 215 | 8.02e-12 | 65.5 |
| MS.gene022324.t1 | AT1G63380 | 29.091 | 220 | 120 | 8 | 39 | 247 | 23 | 217 | 8.80e-12 | 65.1 |
| MS.gene022324.t1 | AT1G63380 | 29.358 | 218 | 118 | 8 | 39 | 245 | 19 | 211 | 8.82e-12 | 65.1 |
| MS.gene022324.t1 | AT1G62610 | 28.505 | 214 | 125 | 7 | 39 | 245 | 23 | 215 | 2.27e-11 | 63.9 |
| MS.gene022324.t1 | AT1G62610 | 28.505 | 214 | 125 | 7 | 39 | 245 | 18 | 210 | 2.32e-11 | 63.9 |
| MS.gene022324.t1 | AT3G61220 | 26.159 | 302 | 154 | 12 | 40 | 293 | 9 | 289 | 2.40e-11 | 63.9 |
| MS.gene022324.t1 | AT1G62610 | 28.505 | 214 | 125 | 7 | 39 | 245 | 17 | 209 | 2.44e-11 | 63.9 |
| MS.gene022324.t1 | AT3G61220 | 26.403 | 303 | 152 | 14 | 40 | 293 | 16 | 296 | 2.53e-11 | 63.9 |
| MS.gene022324.t1 | AT1G62610 | 28.505 | 214 | 125 | 7 | 39 | 245 | 19 | 211 | 2.60e-11 | 63.9 |
| MS.gene022324.t1 | AT3G61220 | 26.159 | 302 | 154 | 12 | 40 | 293 | 40 | 320 | 3.34e-11 | 63.9 |
Find 49 sgRNAs with CRISPR-Local
Find 124 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCTAACAGAAATGTTAT+TGG | 0.226965 | 3.4:+74305981 | MS.gene022324:CDS |
| TCCTTGAATATCTGTCTTCT+TGG | 0.228861 | 3.4:-74306022 | None:intergenic |
| TACTTGGAAGGTGGGACTTC+TGG | 0.239228 | 3.4:+74305429 | MS.gene022324:intron |
| ATTGCAGGGCCTAGTGGTTT+TGG | 0.239997 | 3.4:+74304595 | MS.gene022324:CDS |
| TTCTTTGGCATGCAAAATAT+TGG | 0.244050 | 3.4:-74306245 | None:intergenic |
| TGCTTATGCTAAATCAAAAT+TGG | 0.286899 | 3.4:+74306222 | MS.gene022324:CDS |
| GTATCTCAGTGTTGCCTTCA+TGG | 0.358808 | 3.4:-74304563 | None:intergenic |
| GAGTTGTCTAGCTATTTCTT+TGG | 0.372604 | 3.4:-74306260 | None:intergenic |
| AGATACTTAGCAGGAATTGC+AGG | 0.374017 | 3.4:+74304580 | MS.gene022324:CDS |
| GAAGGTGGGACTTCTGGTAT+TGG | 0.399438 | 3.4:+74305435 | MS.gene022324:intron |
| TCTCACAAGGGGTTAATAAC+AGG | 0.400619 | 3.4:+74306498 | MS.gene022324:CDS |
| AACAGAATAACCTCAGCATT+TGG | 0.410874 | 3.4:-74305564 | None:intergenic |
| TGGTATATTCTCTCAAGATT+TGG | 0.411347 | 3.4:+74305807 | MS.gene022324:CDS |
| TATCTCAGTGTTGCCTTCAT+GGG | 0.418442 | 3.4:-74304562 | None:intergenic |
| AATCAGAAGCTCAAAAGTTA+TGG | 0.420732 | 3.4:+74307008 | MS.gene022324:CDS |
| TTTCTTCTGTCATACATAGC+TGG | 0.434149 | 3.4:+74306058 | MS.gene022324:CDS |
| ACATTTGCCACAAATTACTT+AGG | 0.444013 | 3.4:+74305856 | MS.gene022324:CDS |
| AAATGTACCTAAGTAATTTG+TGG | 0.453221 | 3.4:-74305863 | None:intergenic |
| ATTCCTGTCTTTACAATACC+TGG | 0.456513 | 3.4:-74306468 | None:intergenic |
| GTGGAGTCGTTTGTGAATCA+AGG | 0.463999 | 3.4:-74307044 | None:intergenic |
| AACTTCATATTGTTGCTTGG+TGG | 0.468416 | 3.4:-74307063 | None:intergenic |
| TGTTTCAAAGACATACTCAA+TGG | 0.476615 | 3.4:+74306099 | MS.gene022324:CDS |
| ACTTACATTAGAATATTAAG+TGG | 0.495604 | 3.4:-74305654 | None:intergenic |
| TTCTTCTGTCATACATAGCT+GGG | 0.501675 | 3.4:+74306059 | MS.gene022324:CDS |
| AAATGCTGAGGTTATTCTGT+TGG | 0.509859 | 3.4:+74305566 | MS.gene022324:CDS |
| GGTGATTAGAGCGGTTAGAC+CGG | 0.516299 | 3.4:-74304671 | None:intergenic |
| CATCCAGGTATTGTAAAGAC+AGG | 0.525444 | 3.4:+74306465 | MS.gene022324:CDS |
| TCCAAGAAGACAGATATTCA+AGG | 0.529268 | 3.4:+74306021 | MS.gene022324:CDS |
| ACTATCAATGCAGTTCATCC+AGG | 0.547059 | 3.4:+74306450 | MS.gene022324:CDS |
| TGGTGCAAGGGACTTGAAGA+AGG | 0.555937 | 3.4:+74305506 | MS.gene022324:CDS |
| AGAGGAGTGAGAATTGTGAT+TGG | 0.559022 | 3.4:+74305486 | MS.gene022324:CDS |
| AGAAATAGCTAGACAACTCA+AGG | 0.563686 | 3.4:+74306264 | MS.gene022324:CDS |
| GAATTTGAGCCAAAACCACT+AGG | 0.563701 | 3.4:-74304604 | None:intergenic |
| GCAACACTGAGATACTTAGC+AGG | 0.577681 | 3.4:+74304571 | MS.gene022324:CDS |
| CATAGCTGGGTGAAGAGACA+TGG | 0.586615 | 3.4:+74306072 | MS.gene022324:CDS |
| TGCATCAACTGCATGTTATG+TGG | 0.598728 | 3.4:+74306901 | MS.gene022324:CDS |
| ATTAGAGCGGTTAGACCGGA+AGG | 0.601271 | 3.4:-74304667 | None:intergenic |
| GATACTTAGCAGGAATTGCA+GGG | 0.603209 | 3.4:+74304581 | MS.gene022324:CDS |
| GGTCTAACCGCTCTAATCAC+CGG | 0.603950 | 3.4:+74304673 | MS.gene022324:CDS |
| GCAGGAATTGCAGGGCCTAG+TGG | 0.613097 | 3.4:+74304589 | MS.gene022324:CDS |
| GTTACTGAAATCGACATCAC+AGG | 0.613835 | 3.4:+74306642 | MS.gene022324:CDS |
| TGAGAATTGTGATTGGTGCA+AGG | 0.621535 | 3.4:+74305493 | MS.gene022324:CDS |
| GAGAATTGTGATTGGTGCAA+GGG | 0.621877 | 3.4:+74305494 | MS.gene022324:CDS |
| GCTAGAGTGTTAGCAAAGAG+AGG | 0.622747 | 3.4:+74305468 | MS.gene022324:CDS |
| AGGAATTATTAAATCTCACA+AGG | 0.623928 | 3.4:+74306485 | MS.gene022324:CDS |
| AAAAGAGAGTCCAAATGCTG+AGG | 0.641600 | 3.4:+74305554 | MS.gene022324:CDS |
| GGAATTATTAAATCTCACAA+GGG | 0.654440 | 3.4:+74306486 | MS.gene022324:CDS |
| AGACTTACCGGTGATTAGAG+CGG | 0.666100 | 3.4:-74304680 | None:intergenic |
| GAATTATTAAATCTCACAAG+GGG | 0.680344 | 3.4:+74306487 | MS.gene022324:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTATAAAATGAAAAAG+TGG | - | chr3.4:74305697-74305716 | None:intergenic | 10.0% |
| !! | TCATATTCATATATAATTAT+AGG | + | chr3.4:74306410-74306429 | MS.gene022324:intron | 10.0% |
| !! | TTAAATTTAAATACTCATAA+TGG | + | chr3.4:74306175-74306194 | MS.gene022324:intron | 10.0% |
| !! | ATTACATAGTAATTTACTAT+AGG | - | chr3.4:74306840-74306859 | None:intergenic | 15.0% |
| !! | ATTGATGTAAACATAAATAT+GGG | + | chr3.4:74306744-74306763 | MS.gene022324:intron | 15.0% |
| !! | AAAATTTAATTAGCAGAGAT+AGG | - | chr3.4:74305090-74305109 | None:intergenic | 20.0% |
| !! | ACTAAACAAACAAAAGAAAA+TGG | - | chr3.4:74305056-74305075 | None:intergenic | 20.0% |
| !! | ACTTACATTAGAATATTAAG+TGG | - | chr3.4:74305657-74305676 | None:intergenic | 20.0% |
| !! | ATATATCTCACAATTATATG+TGG | + | chr3.4:74306866-74306885 | MS.gene022324:intron | 20.0% |
| !! | ATGAATATGAATACTTTAGT+GGG | - | chr3.4:74306400-74306419 | None:intergenic | 20.0% |
| !! | ATTAGCATAGTGAAATAAAA+TGG | + | chr3.4:74305715-74305734 | MS.gene022324:intron | 20.0% |
| !! | CAACTTCATTAATTAATAGT+TGG | - | chr3.4:74305391-74305410 | None:intergenic | 20.0% |
| !! | GATTGATGTAAACATAAATA+TGG | + | chr3.4:74306743-74306762 | MS.gene022324:intron | 20.0% |
| !! | TATGAATATGAATACTTTAG+TGG | - | chr3.4:74306401-74306420 | None:intergenic | 20.0% |
| !! | TCTAATTCTTAATGCATATT+TGG | + | chr3.4:74305885-74305904 | MS.gene022324:intron | 20.0% |
| !! | TTCATATATAATTATAGGCA+AGG | + | chr3.4:74306415-74306434 | MS.gene022324:intron | 20.0% |
| !! | TTGATGTAAACATAAATATG+GGG | + | chr3.4:74306745-74306764 | MS.gene022324:intron | 20.0% |
| !!! | AGTATTTTTTTCATCATTCA+TGG | + | chr3.4:74305364-74305383 | MS.gene022324:intron | 20.0% |
| !!! | ATACAATACTCAAGTTTTTT+GGG | - | chr3.4:74305335-74305354 | None:intergenic | 20.0% |
| !!! | ATTTTATTGAATTGAATTGC+AGG | + | chr3.4:74305952-74305971 | MS.gene022324:intron | 20.0% |
| !!! | GTTAAATTTTGTTAAGCATA+GGG | + | chr3.4:74306682-74306701 | MS.gene022324:intron | 20.0% |
| !!! | TATACAATACTCAAGTTTTT+TGG | - | chr3.4:74305336-74305355 | None:intergenic | 20.0% |
| !!! | TGTTAAATTTTGTTAAGCAT+AGG | + | chr3.4:74306681-74306700 | MS.gene022324:intron | 20.0% |
| !!! | TTTTATGATATGTACACTAA+TGG | + | chr3.4:74305753-74305772 | MS.gene022324:intron | 20.0% |
| ! | AAATGTACCTAAGTAATTTG+TGG | - | chr3.4:74305866-74305885 | None:intergenic | 25.0% |
| ! | AATGAGATTATGAGTGTAAA+TGG | - | chr3.4:74305247-74305266 | None:intergenic | 25.0% |
| ! | ACATAGTAATTTACTATAGG+AGG | - | chr3.4:74306837-74306856 | None:intergenic | 25.0% |
| ! | AGGAATTATTAAATCTCACA+AGG | + | chr3.4:74306485-74306504 | MS.gene022324:CDS | 25.0% |
| ! | ATAGTACAATTACATTGTTG+TGG | + | chr3.4:74306793-74306812 | MS.gene022324:intron | 25.0% |
| ! | ATGAGATTATGAGTGTAAAT+GGG | - | chr3.4:74305246-74305265 | None:intergenic | 25.0% |
| ! | CATAGTAATTTACTATAGGA+GGG | - | chr3.4:74306836-74306855 | None:intergenic | 25.0% |
| ! | CTTTGATGCTATGAAAAATA+AGG | - | chr3.4:74306623-74306642 | None:intergenic | 25.0% |
| ! | GAATTATTAAATCTCACAAG+GGG | + | chr3.4:74306487-74306506 | MS.gene022324:CDS | 25.0% |
| ! | GATGATCAATAAAATGAACA+AGG | - | chr3.4:74305288-74305307 | None:intergenic | 25.0% |
| ! | GGAATTATTAAATCTCACAA+GGG | + | chr3.4:74306486-74306505 | MS.gene022324:CDS | 25.0% |
| ! | TGCTTATGCTAAATCAAAAT+TGG | + | chr3.4:74306222-74306241 | MS.gene022324:CDS | 25.0% |
| ! | TTTGCTAACAGAAATGTTAT+TGG | + | chr3.4:74305981-74306000 | MS.gene022324:CDS | 25.0% |
| !! | AAATTTTACAGAAACAATGC+TGG | + | chr3.4:74305787-74305806 | MS.gene022324:intron | 25.0% |
| !!! | AATGAACAAGGTGTATTTTT+TGG | - | chr3.4:74305276-74305295 | None:intergenic | 25.0% |
| !!! | ATCGTTGTTGATTTTTTACT+TGG | + | chr3.4:74305413-74305432 | MS.gene022324:intron | 25.0% |
| !!! | ATGAACAAGGTGTATTTTTT+GGG | - | chr3.4:74305275-74305294 | None:intergenic | 25.0% |
| !!! | GTGTGTATGAGATTTTTTTT+TGG | - | chr3.4:74304771-74304790 | None:intergenic | 25.0% |
| !!! | TTGTTGATTTTTTACTTGGA+AGG | + | chr3.4:74305417-74305436 | MS.gene022324:intron | 25.0% |
| AATCAGAAGCTCAAAAGTTA+TGG | + | chr3.4:74307008-74307027 | MS.gene022324:CDS | 30.0% | |
| ACAAAACTTTCTATGCATGT+TGG | + | chr3.4:74306373-74306392 | MS.gene022324:intron | 30.0% | |
| ACATTTGCCACAAATTACTT+AGG | + | chr3.4:74305856-74305875 | MS.gene022324:CDS | 30.0% | |
| ATCATAGTGAGTTCATTTAG+TGG | + | chr3.4:74305909-74305928 | MS.gene022324:intron | 30.0% | |
| TAAACCATACATAACCAACT+TGG | - | chr3.4:74304816-74304835 | None:intergenic | 30.0% | |
| TATCTCACAATTATATGTGG+TGG | + | chr3.4:74306869-74306888 | MS.gene022324:intron | 30.0% | |
| TCATAGTGAGTTCATTTAGT+GGG | + | chr3.4:74305910-74305929 | MS.gene022324:intron | 30.0% | |
| TGAAAAATAAGGAATCTGCA+TGG | - | chr3.4:74306612-74306631 | None:intergenic | 30.0% | |
| TGAGTCATTCTTTCTCATAA+AGG | + | chr3.4:74304874-74304893 | MS.gene022324:intron | 30.0% | |
| TGGTATATTCTCTCAAGATT+TGG | + | chr3.4:74305807-74305826 | MS.gene022324:CDS | 30.0% | |
| TGTTTCAAAGACATACTCAA+TGG | + | chr3.4:74306099-74306118 | MS.gene022324:CDS | 30.0% | |
| TTCTTTGGCATGCAAAATAT+TGG | - | chr3.4:74306248-74306267 | None:intergenic | 30.0% | |
| ! | TTACTTGTTAAGCTTTCACT+TGG | + | chr3.4:74305124-74305143 | MS.gene022324:intron | 30.0% |
| !!! | TGATTTTTTACTTGGAAGGT+GGG | + | chr3.4:74305421-74305440 | MS.gene022324:intron | 30.0% |
| !!! | TTGATTTTTTACTTGGAAGG+TGG | + | chr3.4:74305420-74305439 | MS.gene022324:intron | 30.0% |
| AAAGTCATTGCAACTTTGCA+GGG | - | chr3.4:74306715-74306734 | None:intergenic | 35.0% | |
| AAATGCTGAGGTTATTCTGT+TGG | + | chr3.4:74305566-74305585 | MS.gene022324:CDS | 35.0% | |
| AACAGAATAACCTCAGCATT+TGG | - | chr3.4:74305567-74305586 | None:intergenic | 35.0% | |
| AGAAATAGCTAGACAACTCA+AGG | + | chr3.4:74306264-74306283 | MS.gene022324:CDS | 35.0% | |
| ATCACATTGCTACAAGAAAG+TGG | + | chr3.4:74306348-74306367 | MS.gene022324:intron | 35.0% | |
| ATTCCTGTCTTTACAATACC+TGG | - | chr3.4:74306471-74306490 | None:intergenic | 35.0% | |
| CTGTGCTAAATTGTAGTACT+TGG | + | chr3.4:74304919-74304938 | MS.gene022324:intron | 35.0% | |
| CTTTGAGTCCAAAAACAGAA+GGG | + | chr3.4:74306924-74306943 | MS.gene022324:CDS | 35.0% | |
| GAGACCAAAATCCAACAAAA+AGG | - | chr3.4:74305213-74305232 | None:intergenic | 35.0% | |
| GTTGCAATGACTTTGATTGA+TGG | + | chr3.4:74306721-74306740 | MS.gene022324:intron | 35.0% | |
| TATGCGTATATAGTTGCTTG+AGG | - | chr3.4:74306776-74306795 | None:intergenic | 35.0% | |
| TCCAAGAAGACAGATATTCA+AGG | + | chr3.4:74306021-74306040 | MS.gene022324:CDS | 35.0% | |
| TCCTTGAATATCTGTCTTCT+TGG | - | chr3.4:74306025-74306044 | None:intergenic | 35.0% | |
| TTCACTTGGCAAAAATTGCA+TGG | + | chr3.4:74305138-74305157 | MS.gene022324:intron | 35.0% | |
| TTCTTCTGTCATACATAGCT+GGG | + | chr3.4:74306059-74306078 | MS.gene022324:CDS | 35.0% | |
| TTTCTTCTGTCATACATAGC+TGG | + | chr3.4:74306058-74306077 | MS.gene022324:CDS | 35.0% | |
| TTTGAGTCCAAAAACAGAAG+GGG | + | chr3.4:74306925-74306944 | MS.gene022324:CDS | 35.0% | |
| ! | AATGGTTTTTGCAGCTACAA+TGG | + | chr3.4:74306193-74306212 | MS.gene022324:intron | 35.0% |
| ! | CATGCCTTTTTGTTGGATTT+TGG | + | chr3.4:74305206-74305225 | MS.gene022324:intron | 35.0% |
| ! | CTATACACATGCCTTTTTGT+TGG | + | chr3.4:74305199-74305218 | MS.gene022324:intron | 35.0% |
| ! | TGTTCTTCTTTTCTTCCTTC+CGG | + | chr3.4:74304652-74304671 | MS.gene022324:CDS | 35.0% |
| !! | GAGTTGTCTAGCTATTTCTT+TGG | - | chr3.4:74306263-74306282 | None:intergenic | 35.0% |
| !! | TTGACCAAGTTGGTTATGTA+TGG | + | chr3.4:74304809-74304828 | MS.gene022324:intron | 35.0% |
| AAAAGAGAGTCCAAATGCTG+AGG | + | chr3.4:74305554-74305573 | MS.gene022324:CDS | 40.0% | |
| AATTATATGTGGTGGTCCTC+AGG | + | chr3.4:74306877-74306896 | MS.gene022324:intron | 40.0% | |
| ACTATCAATGCAGTTCATCC+AGG | + | chr3.4:74306450-74306469 | MS.gene022324:CDS | 40.0% | |
| ACTGAATTCTCAAGGTCAAG+AGG | - | chr3.4:74304715-74304734 | None:intergenic | 40.0% | |
| AGAGGAGTGAGAATTGTGAT+TGG | + | chr3.4:74305486-74305505 | MS.gene022324:CDS | 40.0% | |
| AGATACTTAGCAGGAATTGC+AGG | + | chr3.4:74304580-74304599 | MS.gene022324:CDS | 40.0% | |
| ATTATATGTGGTGGTCCTCA+GGG | + | chr3.4:74306878-74306897 | MS.gene022324:intron | 40.0% | |
| CAAAGTCATTGCAACTTTGC+AGG | - | chr3.4:74306716-74306735 | None:intergenic | 40.0% | |
| GAATTTGAGCCAAAACCACT+AGG | - | chr3.4:74304607-74304626 | None:intergenic | 40.0% | |
| GAGAATTGTGATTGGTGCAA+GGG | + | chr3.4:74305494-74305513 | MS.gene022324:CDS | 40.0% | |
| GATACTTAGCAGGAATTGCA+GGG | + | chr3.4:74304581-74304600 | MS.gene022324:CDS | 40.0% | |
| GTTACTGAAATCGACATCAC+AGG | + | chr3.4:74306642-74306661 | MS.gene022324:CDS | 40.0% | |
| TCTCACAAGGGGTTAATAAC+AGG | + | chr3.4:74306498-74306517 | MS.gene022324:CDS | 40.0% | |
| TGAGAATTGTGATTGGTGCA+AGG | + | chr3.4:74305493-74305512 | MS.gene022324:CDS | 40.0% | |
| TGCATCAACTGCATGTTATG+TGG | + | chr3.4:74306901-74306920 | MS.gene022324:CDS | 40.0% | |
| TGTATATCTGTGCACAACTG+CGG | - | chr3.4:74304740-74304759 | None:intergenic | 40.0% | |
| ! | CATCCAGGTATTGTAAAGAC+AGG | + | chr3.4:74306465-74306484 | MS.gene022324:CDS | 40.0% |
| ! | GCTTTGAGTCCAAAAACAGA+AGG | + | chr3.4:74306923-74306942 | MS.gene022324:CDS | 40.0% |
| ACACGCTTTCTTGACCAAGT+TGG | + | chr3.4:74304799-74304818 | MS.gene022324:intron | 45.0% | |
| AGACTTACCGGTGATTAGAG+CGG | - | chr3.4:74304683-74304702 | None:intergenic | 45.0% | |
| ATTCTCAAGGTCAAGAGGAC+TGG | - | chr3.4:74304710-74304729 | None:intergenic | 45.0% | |
| CAAAAACAGAAGGGGTGAGT+GGG | + | chr3.4:74306933-74306952 | MS.gene022324:CDS | 45.0% | |
| CATTGCAACTTTGCAGGGTT+TGG | - | chr3.4:74306710-74306729 | None:intergenic | 45.0% | |
| CTGAAATCGACATCACAGGT+TGG | + | chr3.4:74306646-74306665 | MS.gene022324:intron | 45.0% | |
| GCAACACTGAGATACTTAGC+AGG | + | chr3.4:74304571-74304590 | MS.gene022324:CDS | 45.0% | |
| GTGGAGTCGTTTGTGAATCA+AGG | - | chr3.4:74307047-74307066 | None:intergenic | 45.0% | |
| ! | AGGACTGGTATGAGACTTAC+CGG | - | chr3.4:74304695-74304714 | None:intergenic | 45.0% |
| ! | GCTAGAGTGTTAGCAAAGAG+AGG | + | chr3.4:74305468-74305487 | MS.gene022324:CDS | 45.0% |
| !!! | TTTCTATTTTTTTTTTTTTT+TGG | + | chr3.4:74304995-74305014 | MS.gene022324:intron | 5.0% |
| ATTAGAGCGGTTAGACCGGA+AGG | - | chr3.4:74304670-74304689 | None:intergenic | 50.0% | |
| CATAGCTGGGTGAAGAGACA+TGG | + | chr3.4:74306072-74306091 | MS.gene022324:CDS | 50.0% | |
| CCAAAAACAGAAGGGGTGAG+TGG | + | chr3.4:74306932-74306951 | MS.gene022324:CDS | 50.0% | |
| CTGCGGAGACTGAATTCTCA+AGG | - | chr3.4:74304723-74304742 | None:intergenic | 50.0% | |
| GAAGGTGGGACTTCTGGTAT+TGG | + | chr3.4:74305435-74305454 | MS.gene022324:intron | 50.0% | |
| GGTCTAACCGCTCTAATCAC+CGG | + | chr3.4:74304673-74304692 | MS.gene022324:CDS | 50.0% | |
| GGTGATTAGAGCGGTTAGAC+CGG | - | chr3.4:74304674-74304693 | None:intergenic | 50.0% | |
| TACTTGGAAGGTGGGACTTC+TGG | + | chr3.4:74305429-74305448 | MS.gene022324:intron | 50.0% | |
| TGGTGCAAGGGACTTGAAGA+AGG | + | chr3.4:74305506-74305525 | MS.gene022324:CDS | 50.0% | |
| ! | ATTGCAGGGCCTAGTGGTTT+TGG | + | chr3.4:74304595-74304614 | MS.gene022324:CDS | 50.0% |
| ! | CCACTCACCCCTTCTGTTTT+TGG | - | chr3.4:74306935-74306954 | None:intergenic | 50.0% |
| GACGTGAGAGTGAGCAAGCA+TGG | + | chr3.4:74304896-74304915 | MS.gene022324:intron | 55.0% | |
| ! | CATGCAGTTGATGCACCCTG+AGG | - | chr3.4:74306896-74306915 | None:intergenic | 55.0% |
| GCAGGAATTGCAGGGCCTAG+TGG | + | chr3.4:74304589-74304608 | MS.gene022324:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 74304565 | 74307081 | 74304565 | ID=MS.gene022324 |
| chr3.4 | mRNA | 74304565 | 74307081 | 74304565 | ID=MS.gene022324.t1;Parent=MS.gene022324 |
| chr3.4 | exon | 74304565 | 74304694 | 74304565 | ID=MS.gene022324.t1.exon1;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74304565 | 74304694 | 74304565 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| chr3.4 | exon | 74305439 | 74305670 | 74305439 | ID=MS.gene022324.t1.exon2;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74305439 | 74305670 | 74305439 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| chr3.4 | exon | 74305798 | 74305877 | 74305798 | ID=MS.gene022324.t1.exon3;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74305798 | 74305877 | 74305798 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| chr3.4 | exon | 74305974 | 74306127 | 74305974 | ID=MS.gene022324.t1.exon4;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74305974 | 74306127 | 74305974 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| chr3.4 | exon | 74306207 | 74306285 | 74306207 | ID=MS.gene022324.t1.exon5;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74306207 | 74306285 | 74306207 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| chr3.4 | exon | 74306432 | 74306519 | 74306432 | ID=MS.gene022324.t1.exon6;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74306432 | 74306519 | 74306432 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| chr3.4 | exon | 74306617 | 74306663 | 74306617 | ID=MS.gene022324.t1.exon7;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74306617 | 74306663 | 74306617 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| chr3.4 | exon | 74306899 | 74307081 | 74306899 | ID=MS.gene022324.t1.exon8;Parent=MS.gene022324.t1 |
| chr3.4 | CDS | 74306899 | 74307081 | 74306899 | ID=cds.MS.gene022324.t1;Parent=MS.gene022324.t1 |
| Gene Sequence |
| Protein sequence |
