AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025378

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025378.t1 MTR_2g044810 96.255 534 20 0 1 534 1 534 0.0 1058
MS.gene025378.t1 MTR_4g124520 67.286 538 155 5 1 534 1 521 0.0 694
MS.gene025378.t1 MTR_2g044810 98.814 253 3 0 282 534 213 465 0.0 538
MS.gene025378.t1 MTR_2g044810 93.427 213 14 0 1 213 1 213 2.77e-132 393
MS.gene025378.t1 MTR_7g108905 43.519 540 279 9 12 530 18 552 4.01e-148 437
MS.gene025378.t1 MTR_8g042910 43.123 538 255 7 45 534 78 612 1.56e-142 424
MS.gene025378.t1 MTR_7g108900 42.315 527 261 8 42 530 35 556 2.37e-141 419
MS.gene025378.t1 MTR_7g108875 40.813 566 280 10 12 534 11 564 2.59e-138 412
MS.gene025378.t1 MTR_3g010770 42.486 539 259 8 45 534 39 575 6.19e-138 411
MS.gene025378.t1 MTR_2g044880 40.367 545 294 10 1 534 1 525 2.39e-136 405
MS.gene025378.t1 MTR_1g083520 41.250 560 271 11 1 534 1 528 2.82e-135 403
MS.gene025378.t1 MTR_1g088020 42.481 532 262 10 42 534 29 555 4.11e-135 403
MS.gene025378.t1 MTR_1g008140 40.484 578 293 14 4 534 34 607 4.36e-135 405
MS.gene025378.t1 MTR_7g108890 42.458 537 254 9 42 534 37 562 2.47e-133 399
MS.gene025378.t1 MTR_1g054880 39.964 563 284 7 9 534 13 558 5.78e-133 398
MS.gene025378.t1 MTR_4g087980 42.612 467 244 6 78 534 64 516 1.71e-132 395
MS.gene025378.t1 MTR_7g112580 40.481 499 270 8 54 534 53 542 1.55e-131 394
MS.gene025378.t1 MTR_1g088225 42.424 528 258 11 42 530 29 549 2.82e-131 393
MS.gene025378.t1 MTR_3g091380 38.366 563 304 9 8 534 42 597 8.96e-131 394
MS.gene025378.t1 MTR_4g025730 41.959 541 275 12 1 534 1 509 1.12e-130 390
MS.gene025378.t1 MTR_8g023310 42.366 524 270 9 41 534 68 589 9.65e-130 390
MS.gene025378.t1 MTR_4g087970 42.137 496 245 10 67 534 36 517 7.85e-129 386
MS.gene025378.t1 MTR_4g025690 41.065 526 288 10 10 530 1 509 6.23e-128 383
MS.gene025378.t1 MTR_8g104655 40.234 512 278 9 45 534 36 541 1.00e-127 384
MS.gene025378.t1 MTR_3g010780 39.293 509 291 7 40 534 70 574 8.66e-125 377
MS.gene025378.t1 MTR_8g042900 39.453 512 290 7 39 534 74 581 2.50e-124 376
MS.gene025378.t1 MTR_7g050870 38.477 512 295 7 38 534 48 554 2.96e-123 373
MS.gene025378.t1 MTR_7g050950 41.053 475 260 7 76 534 83 553 2.97e-123 373
MS.gene025378.t1 MTR_3g033690 39.406 505 289 10 39 534 30 526 5.59e-122 369
MS.gene025378.t1 MTR_3g008640 38.933 506 291 10 39 534 30 527 7.43e-121 365
MS.gene025378.t1 MTR_3g008700 39.400 500 285 10 45 534 36 527 3.36e-120 364
MS.gene025378.t1 MTR_8g104645 49.555 337 161 3 207 534 36 372 4.74e-120 358
MS.gene025378.t1 MTR_3g008720 38.491 530 309 10 12 534 8 527 5.41e-120 363
MS.gene025378.t1 MTR_7g050980 39.619 472 264 7 78 534 90 555 1.10e-119 363
MS.gene025378.t1 MTR_4g025670 44.444 432 229 5 107 534 65 489 1.49e-119 361
MS.gene025378.t1 MTR_7g053360 38.356 511 287 9 39 534 30 527 4.38e-119 361
MS.gene025378.t1 MTR_4g035980 40.198 505 282 10 45 534 40 539 9.06e-119 361
MS.gene025378.t1 MTR_7g112590 36.268 568 310 11 11 533 22 582 1.08e-118 362
MS.gene025378.t1 MTR_1g083470 36.852 521 292 9 38 530 55 566 2.22e-117 358
MS.gene025378.t1 MTR_1g083500 36.852 521 292 9 38 530 55 566 2.22e-117 358
MS.gene025378.t1 MTR_1g083490 37.087 515 299 7 38 530 60 571 2.76e-117 358
MS.gene025378.t1 MTR_1g083510 35.626 567 316 10 11 533 21 582 8.08e-116 355
MS.gene025378.t1 MTR_4g005170 36.699 515 301 7 38 530 60 571 1.01e-115 356
MS.gene025378.t1 MTR_4g005180 36.505 515 302 7 38 530 42 553 7.95e-115 352
MS.gene025378.t1 MTR_0458s0030 57.795 263 111 0 272 534 52 314 1.60e-112 337
MS.gene025378.t1 MTR_1g088040 57.795 263 111 0 272 534 52 314 1.60e-112 337
MS.gene025378.t1 MTR_1g086210 36.940 536 291 11 40 534 40 569 2.00e-112 345
MS.gene025378.t1 MTR_8g023150 40.307 521 273 14 39 534 75 582 5.12e-109 337
MS.gene025378.t1 MTR_7g110560 36.182 550 314 15 8 533 12 548 3.37e-107 331
MS.gene025378.t1 MTR_1g086190 35.766 548 302 15 24 533 27 562 3.82e-106 329
MS.gene025378.t1 MTR_4g124550 34.982 546 308 9 22 534 62 593 3.07e-104 325
MS.gene025378.t1 MTR_8g104580 36.133 512 266 10 45 534 28 500 1.02e-102 318
MS.gene025378.t1 MTR_7g110440 34.717 530 305 10 41 530 42 570 3.32e-99 311
MS.gene025378.t1 MTR_8g093460 35.632 522 297 12 39 533 41 550 1.88e-97 306
MS.gene025378.t1 MTR_3g095920 35.000 560 319 15 8 533 15 563 2.87e-96 303
MS.gene025378.t1 MTR_2g044660 43.787 338 184 3 199 534 299 632 9.14e-95 301
MS.gene025378.t1 MTR_7g110490 37.013 462 252 8 107 529 108 569 1.09e-94 300
MS.gene025378.t1 MTR_7g110510 37.013 462 252 8 107 529 108 569 1.09e-94 300
MS.gene025378.t1 MTR_2g436040 32.874 508 309 9 44 530 46 542 5.11e-90 288
MS.gene025378.t1 MTR_4g120290 32.197 528 320 10 35 533 53 571 7.37e-90 287
MS.gene025378.t1 MTR_4g120280 31.731 520 321 8 41 533 59 571 1.17e-89 287
MS.gene025378.t1 MTR_0618s0020 52.863 227 106 1 309 534 2 228 3.87e-88 271
MS.gene025378.t1 MTR_2g436050 31.021 519 333 9 35 533 50 563 4.73e-83 269
MS.gene025378.t1 MTR_8g088610 40.909 330 186 6 207 530 46 372 3.54e-82 261
MS.gene025378.t1 MTR_7g110420 35.158 475 257 10 107 530 108 582 8.17e-81 264
MS.gene025378.t1 MTR_7g107180 41.987 312 168 5 225 534 7 307 3.66e-79 251
MS.gene025378.t1 MTR_5g083620 41.720 314 174 4 226 534 47 356 1.37e-78 251
MS.gene025378.t1 MTR_2g105560 39.744 312 173 5 225 534 41 339 1.59e-75 242
MS.gene025378.t1 MTR_0458s0010 33.712 528 269 15 42 530 29 514 1.28e-74 246
MS.gene025378.t1 MTR_7g111560 30.935 556 327 15 8 533 27 555 6.21e-72 239
MS.gene025378.t1 MTR_7g110430 32.112 464 240 10 107 530 108 536 3.72e-71 237
MS.gene025378.t1 MTR_6g088810 39.073 302 159 7 233 523 90 377 1.66e-58 199
MS.gene025378.t1 MTR_8g102380 37.908 306 163 7 233 525 88 379 7.95e-57 194
MS.gene025378.t1 MTR_7g092780 36.656 311 176 6 225 525 90 389 4.95e-56 192
MS.gene025378.t1 MTR_6g012800 35.197 304 174 4 224 517 5 295 2.90e-55 188
MS.gene025378.t1 MTR_1g086540 37.621 311 170 7 225 525 50 346 3.15e-55 189
MS.gene025378.t1 MTR_7g011530 35.526 304 173 5 224 517 5 295 1.29e-53 184
MS.gene025378.t1 MTR_6g024165 35.802 324 176 9 214 523 29 334 3.19e-53 184
MS.gene025378.t1 MTR_7g102600 35.045 331 195 7 200 523 76 393 4.14e-50 177
MS.gene025378.t1 MTR_5g012600 32.177 317 189 6 222 526 60 362 4.61e-50 176
MS.gene025378.t1 MTR_5g095490 35.948 306 170 6 231 524 67 358 7.53e-49 172
MS.gene025378.t1 MTR_8g102380 39.044 251 128 5 233 470 88 326 5.79e-48 169
MS.gene025378.t1 MTR_6g042660 33.333 282 165 6 221 497 33 296 9.10e-47 166
MS.gene025378.t1 MTR_2g039530 37.282 287 148 8 226 501 53 318 9.06e-46 164
MS.gene025378.t1 MTR_4g124740 32.051 312 179 10 223 524 52 340 3.80e-43 157
MS.gene025378.t1 MTR_1g033050 31.646 316 194 6 219 527 12 312 6.54e-43 155
MS.gene025378.t1 MTR_5g083590 30.868 311 165 4 225 534 7 268 8.36e-43 154
MS.gene025378.t1 MTR_6g042690 32.759 290 163 7 221 498 35 304 5.07e-40 148
MS.gene025378.t1 MTR_2g033970 32.268 313 186 8 222 525 53 348 3.11e-38 144
MS.gene025378.t1 MTR_5g093970 29.630 324 214 5 201 523 19 329 2.94e-37 140
MS.gene025378.t1 MTR_4g074290 30.719 306 194 7 223 523 69 361 2.40e-36 139
MS.gene025378.t1 MTR_2g073390 30.938 320 200 8 210 523 57 361 6.00e-36 138
MS.gene025378.t1 MTR_5g088300 30.696 316 196 7 210 523 22 316 1.25e-35 136
MS.gene025378.t1 MTR_8g036225 31.834 289 172 6 221 500 29 301 1.35e-35 136
MS.gene025378.t1 MTR_0009s0350 31.047 277 175 5 223 494 69 334 2.28e-34 133
MS.gene025378.t1 MTR_0009s0340 31.047 277 175 5 223 494 69 334 2.28e-34 133
MS.gene025378.t1 MTR_5g088280 30.696 316 196 7 210 523 24 318 1.99e-33 130
MS.gene025378.t1 MTR_5g088290 30.380 316 198 7 210 523 33 328 7.31e-33 129
MS.gene025378.t1 MTR_2g436600 29.781 319 177 13 222 525 52 338 3.53e-31 124
MS.gene025378.t1 MTR_4g051810 28.571 315 205 6 210 523 22 317 1.29e-28 116
MS.gene025378.t1 MTR_6g025590 40.113 177 93 7 214 379 37 211 3.57e-28 113
MS.gene025378.t1 MTR_2g436070 23.607 305 213 8 35 324 50 349 6.21e-17 83.2
MS.gene025378.t1 MTR_0408s0030 33.673 98 56 1 401 498 36 124 1.36e-15 74.7
MS.gene025378.t1 MTR_6g024200 31.250 160 89 5 365 523 137 276 3.74e-15 76.3
MS.gene025378.t1 MTR_7g102580 45.349 86 37 2 299 379 73 153 1.77e-14 71.2
MS.gene025378.t1 MTR_4g130790 30.058 173 106 6 255 418 55 221 7.56e-14 72.4
MS.gene025378.t1 MTR_4g035870 26.486 185 124 6 12 187 8 189 2.29e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025378.t1 AT2G26440 65.107 513 162 4 39 534 35 547 0.0 680
MS.gene025378.t1 AT4G02320 45.248 484 238 10 69 530 36 514 8.26e-140 414
MS.gene025378.t1 AT3G05620 39.273 550 289 8 15 534 7 541 3.28e-139 413
MS.gene025378.t1 AT3G43270 44.813 482 240 6 78 534 45 525 4.42e-138 410
MS.gene025378.t1 AT4G02330 41.053 570 281 10 16 534 6 571 2.63e-137 409
MS.gene025378.t1 AT1G02810 39.964 558 269 8 38 534 25 577 1.10e-134 403
MS.gene025378.t1 AT4G33220 48.210 419 195 4 138 534 105 523 2.15e-134 400
MS.gene025378.t1 AT4G02300 41.481 540 282 9 8 534 14 532 4.71e-132 395
MS.gene025378.t1 AT3G10720 38.692 535 281 7 45 534 83 615 3.83e-131 395
MS.gene025378.t1 AT3G49220 39.362 564 301 11 4 534 41 596 2.06e-129 390
MS.gene025378.t1 AT3G49220 39.362 564 301 11 4 534 86 641 1.27e-128 389
MS.gene025378.t1 AT5G53370 40.467 514 285 10 40 534 74 585 3.12e-127 384
MS.gene025378.t1 AT4G33220 47.761 402 188 4 155 534 1 402 3.76e-127 377
MS.gene025378.t1 AT5G53370 40.467 514 285 10 40 534 110 621 7.39e-127 384
MS.gene025378.t1 AT5G04970 40.074 539 268 12 45 534 88 620 1.58e-126 384
MS.gene025378.t1 AT2G47550 43.413 463 226 6 106 534 98 558 1.22e-125 379
MS.gene025378.t1 AT1G53830 40.490 531 266 10 41 530 62 583 5.94e-125 378
MS.gene025378.t1 AT5G51490 41.420 507 272 8 45 534 38 536 6.59e-124 374
MS.gene025378.t1 AT1G11580 42.727 440 240 6 103 532 118 555 2.70e-123 373
MS.gene025378.t1 AT3G10710 39.509 529 285 10 24 534 50 561 2.80e-123 373
MS.gene025378.t1 AT1G11580 42.727 440 240 6 103 532 139 576 4.78e-123 373
MS.gene025378.t1 AT1G23200 39.888 534 260 13 45 534 38 554 7.30e-122 369
MS.gene025378.t1 AT5G51500 41.245 514 267 12 39 530 36 536 8.76e-122 369
MS.gene025378.t1 AT3G14310 39.259 540 276 10 41 534 59 592 1.17e-121 370
MS.gene025378.t1 AT3G60730 40.202 495 255 9 75 534 31 519 3.96e-121 366
MS.gene025378.t1 AT5G27870 34.982 546 327 8 10 534 24 562 2.70e-119 368
MS.gene025378.t1 AT2G43050 39.416 548 286 13 1 534 1 516 2.48e-118 359
MS.gene025378.t1 AT4G00190 41.331 496 250 12 44 530 7 470 5.91e-117 354
MS.gene025378.t1 AT2G45220 39.042 543 284 17 5 534 3 511 1.35e-116 354
MS.gene025378.t1 AT3G05610 35.009 557 324 7 4 534 21 565 4.06e-116 358
MS.gene025378.t1 AT5G04960 37.328 509 288 9 40 531 67 561 8.87e-115 351
MS.gene025378.t1 AT3G47400 38.703 509 291 7 45 534 88 594 2.38e-112 346
MS.gene025378.t1 AT5G49180 36.313 537 301 9 21 530 44 566 1.24e-111 343
MS.gene025378.t1 AT3G59010 43.891 442 218 11 108 534 102 528 7.57e-110 337
MS.gene025378.t1 AT4G33230 36.994 519 295 10 41 534 95 606 3.13e-109 338
MS.gene025378.t1 AT4G03930 43.108 399 215 5 143 534 131 524 6.25e-109 335
MS.gene025378.t1 AT1G11590 41.570 433 238 6 112 534 97 524 1.15e-108 334
MS.gene025378.t1 AT1G53840 36.154 520 293 10 39 528 71 581 1.34e-108 336
MS.gene025378.t1 AT2G26450 36.257 513 298 10 41 534 109 611 7.66e-108 335
MS.gene025378.t1 AT5G20860 37.179 546 309 16 10 534 6 538 2.92e-107 331
MS.gene025378.t1 AT3G10720 52.510 259 123 0 276 534 1 259 1.05e-106 320
MS.gene025378.t1 AT3G27980 43.038 395 217 5 143 534 108 497 1.96e-106 328
MS.gene025378.t1 AT3G06830 36.122 526 290 12 40 530 50 564 2.50e-103 322
MS.gene025378.t1 AT3G14300 37.132 509 289 13 39 523 457 958 4.35e-102 329
MS.gene025378.t1 AT5G09760 35.490 510 297 14 39 522 43 546 1.51e-89 285
MS.gene025378.t1 AT5G09760 35.490 510 297 14 39 522 69 572 2.48e-89 286
MS.gene025378.t1 AT1G11370 53.112 241 113 0 225 465 46 286 9.35e-89 275
MS.gene025378.t1 AT5G64640 34.038 520 306 13 38 527 90 602 2.66e-87 281
MS.gene025378.t1 AT4G15980 45.130 308 168 1 225 531 392 699 6.20e-87 283
MS.gene025378.t1 AT5G20860 34.286 525 283 15 10 513 6 489 9.59e-87 277
MS.gene025378.t1 AT4G15980 45.130 308 168 1 225 531 402 709 1.34e-86 282
MS.gene025378.t1 AT3G62170 41.987 312 177 3 226 534 278 588 1.57e-77 255
MS.gene025378.t1 AT2G47040 43.175 315 171 4 225 534 284 595 7.76e-76 251
MS.gene025378.t1 AT2G47030 41.270 315 177 4 225 534 277 588 1.59e-74 247
MS.gene025378.t1 AT3G29090 37.898 314 169 6 223 523 5 305 2.49e-58 197
MS.gene025378.t1 AT1G05310 36.508 315 179 5 220 524 86 389 2.06e-56 194
MS.gene025378.t1 AT2G36710 36.280 328 189 6 208 528 76 390 1.01e-55 192
MS.gene025378.t1 AT5G19730 37.294 303 165 6 233 524 94 382 1.15e-55 192
MS.gene025378.t1 AT5G47500 32.012 328 190 7 208 523 51 357 4.08e-49 174
MS.gene025378.t1 AT2G36700 34.426 305 183 5 224 523 41 333 3.13e-48 170
MS.gene025378.t1 AT2G21610 34.868 304 175 8 224 524 50 333 5.13e-48 170
MS.gene025378.t1 AT2G21610 34.868 304 175 8 224 524 55 338 5.28e-48 170
MS.gene025378.t1 AT5G55590 34.395 314 180 6 223 524 80 379 1.83e-46 167
MS.gene025378.t1 AT3G17060 32.476 311 184 6 222 523 43 336 2.32e-46 166
MS.gene025378.t1 AT5G07430 31.532 333 206 7 195 523 41 355 4.56e-43 157
MS.gene025378.t1 AT1G44980 43.915 189 100 1 207 389 54 242 3.28e-42 151
MS.gene025378.t1 AT5G55590 34.733 262 147 5 223 472 80 329 5.90e-41 150
MS.gene025378.t1 AT3G24130 30.721 319 195 6 222 529 31 334 9.99e-41 150
MS.gene025378.t1 AT5G18990 33.559 295 169 8 216 498 21 300 7.05e-39 145
MS.gene025378.t1 AT5G07420 30.820 305 193 5 223 523 65 355 3.71e-35 135
MS.gene025378.t1 AT5G61680 31.212 330 198 11 201 523 51 358 3.74e-35 135
MS.gene025378.t1 AT5G26810 31.804 327 189 8 216 523 22 333 1.94e-34 133
MS.gene025378.t1 AT1G69940 29.814 322 203 9 208 523 51 355 4.95e-34 132
MS.gene025378.t1 AT5G07410 29.814 322 203 9 208 523 51 355 7.21e-34 132
MS.gene025378.t1 AT2G19150 29.653 317 198 10 223 530 39 339 4.46e-31 124
MS.gene025378.t1 AT2G19150 32.157 255 150 10 223 468 39 279 2.55e-28 114
MS.gene025378.t1 AT3G49220 30.976 297 171 10 40 313 131 416 5.90e-28 116
MS.gene025378.t1 AT2G47280 28.065 310 195 7 225 523 35 327 1.40e-27 114
MS.gene025378.t1 AT2G47670 29.714 175 111 4 28 191 31 204 1.20e-12 67.8
MS.gene025378.t1 AT5G62360 29.747 158 102 4 41 191 44 199 8.45e-11 62.0

Find 87 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTAACCAAACTATTATT+TGG 0.162094 2.2:+42315854 None:intergenic
GGGACAATAGATTTCATATT+TGG 0.171421 2.2:-42314371 MS.gene025378:CDS
TATGGTGAATATGAAAATTA+TGG 0.192417 2.2:-42314044 MS.gene025378:CDS
GATGGTCTTGATTCTGCTTC+TGG 0.229635 2.2:-42316124 MS.gene025378:CDS
ATCGCGAATGCGACATTTAC+GGG 0.267997 2.2:-42314391 MS.gene025378:CDS
AGAAGGAAGTTTATGATGTT+TGG 0.270843 2.2:+42316388 None:intergenic
AGTGTTTCTGAGTTTATTAT+TGG 0.272877 2.2:-42313945 MS.gene025378:CDS
ATGAGTTGAAACAAAGTTTA+GGG 0.273837 2.2:+42316440 None:intergenic
AATGAGTTGAAACAAAGTTT+AGG 0.277122 2.2:+42316439 None:intergenic
TAGCGATAATCGAACAGTTT+TGG 0.287476 2.2:+42314228 None:intergenic
TTTGTTCGAGGAACGGATTC+CGG 0.293719 2.2:+42316210 None:intergenic
ATTAGAGAGCTTTGTGGTTT+CGG 0.295297 2.2:+42316342 None:intergenic
CTTTGCTCCAAATAATAGTT+TGG 0.297100 2.2:-42315861 MS.gene025378:CDS
ATCAAAAGGGTTGGATAAAA+TGG 0.305630 2.2:-42314097 MS.gene025378:CDS
ACTTTCAGATCTGCAACTTT+AGG 0.311138 2.2:-42315695 MS.gene025378:intron
TGGGTTTATGTTTATTGATA+TGG 0.321135 2.2:+42316408 None:intergenic
ATAATAGAATCCACAAGAAT+TGG 0.334084 2.2:+42316091 None:intergenic
GAAGGAAGTTTATGATGTTT+GGG 0.337339 2.2:+42316389 None:intergenic
TATCGCGAATGCGACATTTA+CGG 0.340554 2.2:-42314392 MS.gene025378:CDS
TTGACCAGGTAATGGCATTC+TGG 0.352368 2.2:+42314310 None:intergenic
GAGATTCCCGTGTCTTCGTC+AGG 0.358299 2.2:+42314254 None:intergenic
GATACAACTAGCATTTCATT+TGG 0.363269 2.2:+42315929 None:intergenic
ATGGGTTGGTTATCATTTGA+TGG 0.392958 2.2:-42313991 MS.gene025378:CDS
ACCTGAGCCTTCAAATCAAA+AGG 0.410993 2.2:-42316017 MS.gene025378:CDS
GCAACTGCTTGATGTTTCTC+TGG 0.415712 2.2:+42314506 None:intergenic
AGTTTATTATTGGTGATCAA+TGG 0.423850 2.2:-42313935 MS.gene025378:CDS
ACTTCAGTTCCTTATGATGA+TGG 0.435256 2.2:-42313903 MS.gene025378:CDS
AGATTGATAATCGTGTTGAA+TGG 0.440086 2.2:-42314010 MS.gene025378:CDS
ATAGTTATTTATGTTAAAGA+AGG 0.446972 2.2:-42315833 MS.gene025378:CDS
ATGGTCAAATGATCAAGGTT+TGG 0.447423 2.2:-42314078 MS.gene025378:CDS
GCTTTATTCAAACTCTAGTA+AGG 0.454494 2.2:-42314198 MS.gene025378:CDS
CTGTGATAACCGCGCAATCA+CGG 0.455996 2.2:-42314283 MS.gene025378:CDS
CTCCAAATAATAGTTTGGTT+AGG 0.464459 2.2:-42315856 MS.gene025378:CDS
TAGTTATTTATGTTAAAGAA+GGG 0.479130 2.2:-42315832 MS.gene025378:CDS
TGTCAAATTCCATCATCATA+AGG 0.481413 2.2:+42313894 None:intergenic
TTGAAACAAAGTTTAGGGTA+TGG 0.481564 2.2:+42316445 None:intergenic
CTAGTTGAAGTGAATGAAGA+AGG 0.483535 2.2:+42316371 None:intergenic
ATAAAATGGTCAAATGATCA+AGG 0.490510 2.2:-42314083 MS.gene025378:CDS
TGTGATAACCGCGCAATCAC+GGG 0.492798 2.2:-42314282 MS.gene025378:CDS
GTTGTATCTGCAGATGGAAG+TGG 0.497219 2.2:-42315914 MS.gene025378:CDS
GGTAATAGAAGTGTTGTTGA+TGG 0.501117 2.2:-42315725 MS.gene025378:CDS
AGAAGAGGATTGTAAAGAGA+AGG 0.502018 2.2:+42316560 None:intergenic
GGTATTGAATGATGAGGTTG+TGG 0.502841 2.2:+42316507 None:intergenic
AGTAAGGTCAAGAGTTACCT+TGG 0.505459 2.2:-42314182 MS.gene025378:CDS
GATTGATAATCGTGTTGAAT+GGG 0.506162 2.2:-42314009 MS.gene025378:CDS
ACAGTAAATTGACCAGGTAA+TGG 0.511755 2.2:+42314302 None:intergenic
TGATGAGGTTGTGGATGTGT+TGG 0.513524 2.2:+42316516 None:intergenic
GAAAAGATTAGAGAGCTTTG+TGG 0.521305 2.2:+42316336 None:intergenic
TCATTGAAAAGATCGTTATC+CGG 0.521388 2.2:-42316229 MS.gene025378:CDS
AAAACTAACATTGTGATGCT+TGG 0.522551 2.2:-42315776 MS.gene025378:CDS
GAGGTAATGTGAGTGAGAAG+AGG 0.527927 2.2:+42316545 None:intergenic
TGTTGGTTAGGGCTGCACTT+AGG 0.533033 2.2:+42316161 None:intergenic
GTAAGGTCAAGAGTTACCTT+GGG 0.536463 2.2:-42314181 MS.gene025378:CDS
TACATATAAACCAATTCTTG+TGG 0.539630 2.2:-42316101 MS.gene025378:CDS
CGATTATCAATCTTTGAACC+AGG 0.546156 2.2:+42314020 None:intergenic
AGAGTTACCTTGGGAGGCCA+TGG 0.549483 2.2:-42314172 MS.gene025378:CDS
ATGTGCAATTTATGGTTACC+AGG 0.561396 2.2:-42314447 MS.gene025378:CDS
GATAATCGTGTTGAATGGGT+TGG 0.569200 2.2:-42314005 MS.gene025378:CDS
AACATTGTGATGCTTGGTGA+TGG 0.570666 2.2:-42315770 MS.gene025378:CDS
TAAAGTTGCAGATCTGAAAG+TGG 0.573525 2.2:+42315697 None:intergenic
CCTGAGCCTTCAAATCAAAA+GGG 0.575156 2.2:-42316016 MS.gene025378:CDS
AGATTCCCGTGTCTTCGTCA+GGG 0.576802 2.2:+42314255 None:intergenic
ATAGCATTTGAGAACAGAGC+TGG 0.580889 2.2:-42314530 MS.gene025378:CDS
ATAGAAGTGTTGTTGATGGA+TGG 0.582279 2.2:-42315721 MS.gene025378:CDS
TTACCTTGGGAGGCCATGGA+GGG 0.584141 2.2:-42314168 MS.gene025378:CDS
GTATCCAGAATGCCATTACC+TGG 0.590515 2.2:-42314314 MS.gene025378:CDS
GTGGATGTGTTGGTGTGAAG+AGG 0.593697 2.2:+42316526 None:intergenic
GATGATGAGTTTGTGAGATG+AGG 0.596471 2.2:+42316582 None:intergenic
AGTTATTTATGTTAAAGAAG+GGG 0.596580 2.2:-42315831 MS.gene025378:CDS
GGATAACCCTGACGAAGACA+CGG 0.597200 2.2:-42314261 MS.gene025378:CDS
AGTGGACATAAAGTGTGTCC+TGG 0.599538 2.2:+42314429 None:intergenic
ATGCTAGTTGTATCTGCAGA+TGG 0.599555 2.2:-42315920 MS.gene025378:CDS
AAACCCTCCATGGCCTCCCA+AGG 0.605902 2.2:+42314165 None:intergenic
AAACTGTTCGATTATCGCTA+CGG 0.609196 2.2:-42314225 MS.gene025378:CDS
AGTGATTCTACTGTGATCAC+TGG 0.609593 2.2:-42315746 MS.gene025378:CDS
GTTACCTTGGGAGGCCATGG+AGG 0.620460 2.2:-42314169 MS.gene025378:CDS
GATAGAATTGTCTAAATGAG+TGG 0.620592 2.2:+42314411 None:intergenic
TATTCGATGTTCCAACGTCG+TGG 0.623832 2.2:+42316314 None:intergenic
GATAACCCTGACGAAGACAC+GGG 0.624320 2.2:-42314260 MS.gene025378:CDS
AGGTCAAGAGTTACCTTGGG+AGG 0.624790 2.2:-42314178 MS.gene025378:CDS
TTTGTTGGTATTGAATGATG+AGG 0.627640 2.2:+42316501 None:intergenic
AGGGTTATCCCGTGATTGCG+CGG 0.640136 2.2:+42314274 None:intergenic
AGGATCAATTCAAGACTGCA+AGG 0.641298 2.2:-42316275 MS.gene025378:CDS
GAATATGAAAATTATGGACC+TGG 0.647894 2.2:-42314038 MS.gene025378:CDS
GTTATCACAGTAAATTGACC+AGG 0.684037 2.2:+42314296 None:intergenic
GCATTCTTGTAATACTACTG+CGG 0.701105 2.2:+42314343 None:intergenic
AATATCGTAGAGAAACAACG+AGG 0.837126 2.2:-42316295 MS.gene025378:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTATAATATGAAAAGA+GGG + chr2.2:42315325-42315344 None:intergenic 10.0%
!! TAAAATTATAATATGAAAAG+AGG + chr2.2:42315326-42315345 None:intergenic 10.0%
!!! CTTTTCATATTATAATTTTA+TGG - chr2.2:42315326-42315345 MS.gene025378:intron 10.0%
!!! TTAAACTAATTCTTAAATTT+TGG - chr2.2:42315240-42315259 MS.gene025378:intron 10.0%
!!! TTTTAAAATTGATTTTGTTT+TGG - chr2.2:42314978-42314997 MS.gene025378:intron 10.0%
!! ATAGTTATTTATGTTAAAGA+AGG - chr2.2:42314652-42314671 MS.gene025378:intron 15.0%
!! TAATTTCATTGAAATTGATT+TGG + chr2.2:42315208-42315227 None:intergenic 15.0%
!! TAGTTAAAAAAAATTGTCTT+TGG - chr2.2:42315105-42315124 MS.gene025378:intron 15.0%
!! TAGTTATTTATGTTAAAGAA+GGG - chr2.2:42314653-42314672 MS.gene025378:intron 15.0%
!!! AACATTTTCCTAATTTTATT+TGG - chr2.2:42315521-42315540 MS.gene025378:intron 15.0%
!!! ACATTTTCCTAATTTTATTT+GGG - chr2.2:42315522-42315541 MS.gene025378:intron 15.0%
!!! TAATTCTTAAATTTTGGTTT+AGG - chr2.2:42315246-42315265 MS.gene025378:intron 15.0%
!! ACAAATACAATCTTAGAATT+TGG - chr2.2:42315571-42315590 MS.gene025378:intron 20.0%
!! AGTTATTTATGTTAAAGAAG+GGG - chr2.2:42314654-42314673 MS.gene025378:intron 20.0%
!! CAAATACAATCTTAGAATTT+GGG - chr2.2:42315572-42315591 MS.gene025378:intron 20.0%
!! CATATAAATTTCTTCATTTC+AGG - chr2.2:42315141-42315160 MS.gene025378:intron 20.0%
!! TATGGTGAATATGAAAATTA+TGG - chr2.2:42316441-42316460 MS.gene025378:CDS 20.0%
!!! AATATAGATGTTTTATGCTT+AGG - chr2.2:42315021-42315040 MS.gene025378:intron 20.0%
!!! ACAATGTTAGTTTTATAACT+TGG + chr2.2:42314703-42314722 None:intergenic 20.0%
!!! CAAAAGTAAAAGTTTTTTCT+TGG + chr2.2:42315469-42315488 None:intergenic 20.0%
!!! CAAGAAAAAACTTTTACTTT+TGG - chr2.2:42315467-42315486 MS.gene025378:intron 20.0%
!!! CATTTTCCTAATTTTATTTG+GGG - chr2.2:42315523-42315542 MS.gene025378:intron 20.0%
!!! GATGATTTTATTGATCAAAA+GGG - chr2.2:42316375-42316394 MS.gene025378:CDS 20.0%
!!! TGATGATTTTATTGATCAAA+AGG - chr2.2:42316374-42316393 MS.gene025378:CDS 20.0%
!!! TGTTTTGCATAAAGATTTTA+AGG + chr2.2:42314017-42314036 None:intergenic 20.0%
!!! TTTTACAAATGTGCAATTTA+TGG - chr2.2:42316030-42316049 MS.gene025378:CDS 20.0%
! AAAAATCTACTTCTCCAAAA+TGG - chr2.2:42314500-42314519 MS.gene025378:CDS 25.0%
! AAAGACAAAACCAAGAAAAA+AGG + chr2.2:42314832-42314851 None:intergenic 25.0%
! AACAAAAACACATGTTTAGA+TGG - chr2.2:42314343-42314362 MS.gene025378:CDS 25.0%
! AAGACAAAACCAAGAAAAAA+GGG + chr2.2:42314831-42314850 None:intergenic 25.0%
! ACATGGTACACTAATTTATT+TGG + chr2.2:42315855-42315874 None:intergenic 25.0%
! ACTAATTTCATACTATGGAT+TGG - chr2.2:42315755-42315774 MS.gene025378:CDS 25.0%
! ACTTACCATCAAATAAAGAT+TGG + chr2.2:42315066-42315085 None:intergenic 25.0%
! ATAAAATGGTCAAATGATCA+AGG - chr2.2:42316402-42316421 MS.gene025378:CDS 25.0%
! ATAATAGAATCCACAAGAAT+TGG + chr2.2:42314397-42314416 None:intergenic 25.0%
! ATCCTAACCAAACTATTATT+TGG + chr2.2:42314634-42314653 None:intergenic 25.0%
! CTTACCATCAAATAAAGATT+GGG + chr2.2:42315065-42315084 None:intergenic 25.0%
! GAGTAACTAATTTCATACTA+TGG - chr2.2:42315750-42315769 MS.gene025378:CDS 25.0%
! GTTAATCCCCAAATAAAATT+AGG + chr2.2:42315532-42315551 None:intergenic 25.0%
! GTTATCAAAAAGCTTAGATT+TGG - chr2.2:42314522-42314541 MS.gene025378:CDS 25.0%
! TACATATAAACCAATTCTTG+TGG - chr2.2:42314384-42314403 MS.gene025378:CDS 25.0%
! TCAATAATAATGTTCACAAC+TGG - chr2.2:42315701-42315720 MS.gene025378:CDS 25.0%
! TGGGTTTATGTTTATTGATA+TGG + chr2.2:42314080-42314099 None:intergenic 25.0%
!! AAAGTTGGATTTTTGTCATT+TGG - chr2.2:42315791-42315810 MS.gene025378:CDS 25.0%
!! AATATGAAAAGAGGGTATAT+TGG + chr2.2:42315317-42315336 None:intergenic 25.0%
!! AATCTTTATTTGATGGTAAG+TGG - chr2.2:42315065-42315084 MS.gene025378:intron 25.0%
!! AATGAGTTGAAACAAAGTTT+AGG + chr2.2:42314049-42314068 None:intergenic 25.0%
!! AGTGTTTCTGAGTTTATTAT+TGG - chr2.2:42316540-42316559 MS.gene025378:CDS 25.0%
!! AGTTTATTATTGGTGATCAA+TGG - chr2.2:42316550-42316569 MS.gene025378:CDS 25.0%
!! ATGAGTTGAAACAAAGTTTA+GGG + chr2.2:42314048-42314067 None:intergenic 25.0%
!! ATTTTATTGATCAAAAGGGT+TGG - chr2.2:42316379-42316398 MS.gene025378:CDS 25.0%
!! CATCCTTTATTAACCTTTTT+CGG - chr2.2:42315643-42315662 MS.gene025378:intron 25.0%
!! GTTGTTATCTCTTTTCAATT+TGG - chr2.2:42315665-42315684 MS.gene025378:intron 25.0%
!! TTTTCTTTACCCTTTTTTCT+TGG - chr2.2:42314819-42314838 MS.gene025378:intron 25.0%
!! TTTTGGTCAAACATTATTTC+AGG - chr2.2:42315172-42315191 MS.gene025378:intron 25.0%
!!! AAGCTTTTTGATAACCATTT+TGG + chr2.2:42314517-42314536 None:intergenic 25.0%
!!! AAGTTGGATTTTTGTCATTT+GGG - chr2.2:42315792-42315811 MS.gene025378:CDS 25.0%
!!! ATCTAAACATGTGTTTTTGT+TGG + chr2.2:42314344-42314363 None:intergenic 25.0%
AAAACTAACATTGTGATGCT+TGG - chr2.2:42314709-42314728 MS.gene025378:intron 30.0%
AAAGAGATAACAACCGAAAA+AGG + chr2.2:42315659-42315678 None:intergenic 30.0%
AGAAGGAAGTTTATGATGTT+TGG + chr2.2:42314100-42314119 None:intergenic 30.0%
ATCAAAAGGGTTGGATAAAA+TGG - chr2.2:42316388-42316407 MS.gene025378:CDS 30.0%
CAACCGAAAAAGGTTAATAA+AGG + chr2.2:42315649-42315668 None:intergenic 30.0%
CTCCAAATAATAGTTTGGTT+AGG - chr2.2:42314629-42314648 MS.gene025378:intron 30.0%
CTTTGCTCCAAATAATAGTT+TGG - chr2.2:42314624-42314643 MS.gene025378:intron 30.0%
GAAGGAAGTTTATGATGTTT+GGG + chr2.2:42314099-42314118 None:intergenic 30.0%
GAATATGAAAATTATGGACC+TGG - chr2.2:42316447-42316466 MS.gene025378:CDS 30.0%
GATACAACTAGCATTTCATT+TGG + chr2.2:42314559-42314578 None:intergenic 30.0%
GATAGAATTGTCTAAATGAG+TGG + chr2.2:42316077-42316096 None:intergenic 30.0%
GGGACAATAGATTTCATATT+TGG - chr2.2:42316114-42316133 MS.gene025378:CDS 30.0%
GGTGACATTACAATAAAAGT+TGG - chr2.2:42315776-42315795 MS.gene025378:CDS 30.0%
TATTTGTGTCTATCCAAGAT+AGG + chr2.2:42315558-42315577 None:intergenic 30.0%
TCATTGAAAAGATCGTTATC+CGG - chr2.2:42314256-42314275 MS.gene025378:CDS 30.0%
! AGATTGATAATCGTGTTGAA+TGG - chr2.2:42316475-42316494 MS.gene025378:CDS 30.0%
! ATCTCTTTTCAATTTGGACT+TGG - chr2.2:42315671-42315690 MS.gene025378:intron 30.0%
! GATTGATAATCGTGTTGAAT+GGG - chr2.2:42316476-42316495 MS.gene025378:CDS 30.0%
! GCTTTATTCAAACTCTAGTA+AGG - chr2.2:42316287-42316306 MS.gene025378:CDS 30.0%
! GTTTCCCAATCTTTATTTGA+TGG - chr2.2:42315058-42315077 MS.gene025378:intron 30.0%
! TTGAAACAAAGTTTAGGGTA+TGG + chr2.2:42314043-42314062 None:intergenic 30.0%
! TTTGTTGGTATTGAATGATG+AGG + chr2.2:42313987-42314006 None:intergenic 30.0%
!! AAAATGTTAACTTGTGACCT+TGG + chr2.2:42315509-42315528 None:intergenic 30.0%
!! AAATGTTAACTTGTGACCTT+GGG + chr2.2:42315508-42315527 None:intergenic 30.0%
!! AACATGTGTTTTTGTTGGTT+AGG + chr2.2:42314339-42314358 None:intergenic 30.0%
!! ACATGTGTTTTTGTTGGTTA+GGG + chr2.2:42314338-42314357 None:intergenic 30.0%
!! GGTTTGGATACATTGTTTTA+TGG - chr2.2:42316423-42316442 MS.gene025378:CDS 30.0%
!! TTTCATTTTTCAGCTGTATC+TGG - chr2.2:42315910-42315929 MS.gene025378:CDS 30.0%
!! TTTGGTTTAGGTTTTTCACA+AGG - chr2.2:42315258-42315277 MS.gene025378:intron 30.0%
!! TTTTAAGGAACTGAGTTTGT+TGG + chr2.2:42314002-42314021 None:intergenic 30.0%
!!! CATTTCAGGCTAATTTGTTT+TGG - chr2.2:42315155-42315174 MS.gene025378:intron 30.0%
!!! TTGTAACCCTTTTGATTTGA+AGG + chr2.2:42314478-42314497 None:intergenic 30.0%
AAACTGTTCGATTATCGCTA+CGG - chr2.2:42316260-42316279 MS.gene025378:CDS 35.0%
AATATCGTAGAGAAACAACG+AGG - chr2.2:42314190-42314209 MS.gene025378:CDS 35.0%
ACAAAGCACAGAAAAATCCA+AGG + chr2.2:42314860-42314879 None:intergenic 35.0%
ACAGTAAATTGACCAGGTAA+TGG + chr2.2:42316186-42316205 None:intergenic 35.0%
ACTTCAGTTCCTTATGATGA+TGG - chr2.2:42316582-42316601 MS.gene025378:CDS 35.0%
ACTTTCAGATCTGCAACTTT+AGG - chr2.2:42314790-42314809 MS.gene025378:intron 35.0%
AGAAGAGGATTGTAAAGAGA+AGG + chr2.2:42313928-42313947 None:intergenic 35.0%
ATGGTCAAATGATCAAGGTT+TGG - chr2.2:42316407-42316426 MS.gene025378:CDS 35.0%
ATGTGCAATTTATGGTTACC+AGG - chr2.2:42316038-42316057 MS.gene025378:CDS 35.0%
CGATTATCAATCTTTGAACC+AGG + chr2.2:42316468-42316487 None:intergenic 35.0%
CTAGTTGAAGTGAATGAAGA+AGG + chr2.2:42314117-42314136 None:intergenic 35.0%
GAAAAGATTAGAGAGCTTTG+TGG + chr2.2:42314152-42314171 None:intergenic 35.0%
GCATTCTTGTAATACTACTG+CGG + chr2.2:42316145-42316164 None:intergenic 35.0%
GTTATCACAGTAAATTGACC+AGG + chr2.2:42316192-42316211 None:intergenic 35.0%
TAAAGTTGCAGATCTGAAAG+TGG + chr2.2:42314791-42314810 None:intergenic 35.0%
TGACTGTCAATTTGGAAACA+TGG + chr2.2:42315872-42315891 None:intergenic 35.0%
TTATCACGTGACTGTCAATT+TGG + chr2.2:42315880-42315899 None:intergenic 35.0%
! AGCTATGTTTCTTTTGTTCG+AGG + chr2.2:42314290-42314309 None:intergenic 35.0%
! ATTAGAGAGCTTTGTGGTTT+CGG + chr2.2:42314146-42314165 None:intergenic 35.0%
! TAGCGATAATCGAACAGTTT+TGG + chr2.2:42316260-42316279 None:intergenic 35.0%
! TGTTTCTTTTGTTCGAGGAA+CGG + chr2.2:42314285-42314304 None:intergenic 35.0%
!! AATGTTAACTTGTGACCTTG+GGG + chr2.2:42315507-42315526 None:intergenic 35.0%
!! ATAGAAGTGTTGTTGATGGA+TGG - chr2.2:42314764-42314783 MS.gene025378:intron 35.0%
!! ATGGGTTGGTTATCATTTGA+TGG - chr2.2:42316494-42316513 MS.gene025378:CDS 35.0%
!! GGTAATAGAAGTGTTGTTGA+TGG - chr2.2:42314760-42314779 MS.gene025378:intron 35.0%
!! GGTTTTGTCTTTTACTTCCT+TGG - chr2.2:42314840-42314859 MS.gene025378:intron 35.0%
!! TTCCTTAACTTGTGCTCTAA+GGG + chr2.2:42315368-42315387 None:intergenic 35.0%
ACCTGAGCCTTCAAATCAAA+AGG - chr2.2:42314468-42314487 MS.gene025378:CDS 40.0%
AGGATCAATTCAAGACTGCA+AGG - chr2.2:42314210-42314229 MS.gene025378:CDS 40.0%
AGTAAGGTCAAGAGTTACCT+TGG - chr2.2:42316303-42316322 MS.gene025378:CDS 40.0%
AGTGATTCTACTGTGATCAC+TGG - chr2.2:42314739-42314758 MS.gene025378:intron 40.0%
ATAGCATTTGAGAACAGAGC+TGG - chr2.2:42315955-42315974 MS.gene025378:CDS 40.0%
ATGCTAGTTGTATCTGCAGA+TGG - chr2.2:42314565-42314584 MS.gene025378:CDS 40.0%
CCTGAGCCTTCAAATCAAAA+GGG - chr2.2:42314469-42314488 MS.gene025378:CDS 40.0%
GAATCTGACTCACTGAACAA+TGG + chr2.2:42315823-42315842 None:intergenic 40.0%
GATAATCGTGTTGAATGGGT+TGG - chr2.2:42316480-42316499 MS.gene025378:CDS 40.0%
GATGATGAGTTTGTGAGATG+AGG + chr2.2:42313906-42313925 None:intergenic 40.0%
GTAAGGTCAAGAGTTACCTT+GGG - chr2.2:42316304-42316323 MS.gene025378:CDS 40.0%
TATCGCGAATGCGACATTTA+CGG - chr2.2:42316093-42316112 MS.gene025378:CDS 40.0%
! AACATTGTGATGCTTGGTGA+TGG - chr2.2:42314715-42314734 MS.gene025378:intron 40.0%
! GGTATTGAATGATGAGGTTG+TGG + chr2.2:42313981-42314000 None:intergenic 40.0%
! TCTAATCTTTTCCACGACGT+TGG - chr2.2:42314160-42314179 MS.gene025378:CDS 40.0%
! TTTTCAGCTGTATCTGGAGA+TGG - chr2.2:42315916-42315935 MS.gene025378:CDS 40.0%
!! GTTCCTTAACTTGTGCTCTA+AGG + chr2.2:42315369-42315388 None:intergenic 40.0%
!! GTTCTAGAAAAAACCCTCCA+TGG + chr2.2:42316333-42316352 None:intergenic 40.0%
AGTGGACATAAAGTGTGTCC+TGG + chr2.2:42316059-42316078 None:intergenic 45.0%
ATCGCGAATGCGACATTTAC+GGG - chr2.2:42316094-42316113 MS.gene025378:CDS 45.0%
GAGGTAATGTGAGTGAGAAG+AGG + chr2.2:42313943-42313962 None:intergenic 45.0%
GCAACTGCTTGATGTTTCTC+TGG + chr2.2:42315982-42316001 None:intergenic 45.0%
GGGGATTAACTCACCTATCT+TGG - chr2.2:42315542-42315561 MS.gene025378:intron 45.0%
GTATCCAGAATGCCATTACC+TGG - chr2.2:42316171-42316190 MS.gene025378:CDS 45.0%
GTTCCTTAACTTATGCCCCA+AGG - chr2.2:42315489-42315508 MS.gene025378:intron 45.0%
GTTGTATCTGCAGATGGAAG+TGG - chr2.2:42314571-42314590 MS.gene025378:intron 45.0%
TATTCGATGTTCCAACGTCG+TGG + chr2.2:42314174-42314193 None:intergenic 45.0%
TGACCTTGGGGCATAAGTTA+AGG + chr2.2:42315495-42315514 None:intergenic 45.0%
TGATGAGGTTGTGGATGTGT+TGG + chr2.2:42313972-42313991 None:intergenic 45.0%
TGCCCTTAGAGCACAAGTTA+AGG - chr2.2:42315363-42315382 MS.gene025378:intron 45.0%
! GATGGTCTTGATTCTGCTTC+TGG - chr2.2:42314361-42314380 MS.gene025378:CDS 45.0%
! TTGACCAGGTAATGGCATTC+TGG + chr2.2:42316178-42316197 None:intergenic 45.0%
! TTTGTTCGAGGAACGGATTC+CGG + chr2.2:42314278-42314297 None:intergenic 45.0%
!!! CCCTTTTGATTTGAAGGCTC+AGG + chr2.2:42314472-42314491 None:intergenic 45.0%
!!! TTTTCATATTATAATTTTAT+GGG - chr2.2:42315327-42315346 MS.gene025378:intron 5.0%
AGATTCCCGTGTCTTCGTCA+GGG + chr2.2:42316233-42316252 None:intergenic 50.0%
AGGTCAAGAGTTACCTTGGG+AGG - chr2.2:42316307-42316326 MS.gene025378:CDS 50.0%
CTGTGATAACCGCGCAATCA+CGG - chr2.2:42316202-42316221 MS.gene025378:CDS 50.0%
GATAACCCTGACGAAGACAC+GGG - chr2.2:42316225-42316244 MS.gene025378:CDS 50.0%
GGATAACCCTGACGAAGACA+CGG - chr2.2:42316224-42316243 MS.gene025378:CDS 50.0%
TGTGATAACCGCGCAATCAC+GGG - chr2.2:42316203-42316222 MS.gene025378:CDS 50.0%
! GTGGATGTGTTGGTGTGAAG+AGG + chr2.2:42313962-42313981 None:intergenic 50.0%
! TGTTGGTTAGGGCTGCACTT+AGG + chr2.2:42314327-42314346 None:intergenic 50.0%
AGAGTTACCTTGGGAGGCCA+TGG - chr2.2:42316313-42316332 MS.gene025378:CDS 55.0%
AGGGTTATCCCGTGATTGCG+CGG + chr2.2:42316214-42316233 None:intergenic 55.0%
GAGATTCCCGTGTCTTCGTC+AGG + chr2.2:42316234-42316253 None:intergenic 55.0%
TTACCTTGGGAGGCCATGGA+GGG - chr2.2:42316317-42316336 MS.gene025378:CDS 55.0%
AAACCCTCCATGGCCTCCCA+AGG + chr2.2:42316323-42316342 None:intergenic 60.0%
GTTACCTTGGGAGGCCATGG+AGG - chr2.2:42316316-42316335 MS.gene025378:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 42313896 42316611 42313896 ID=MS.gene025378
chr2.2 mRNA 42313896 42316611 42313896 ID=MS.gene025378.t1;Parent=MS.gene025378
chr2.2 exon 42315696 42316611 42315696 ID=MS.gene025378.t1.exon1;Parent=MS.gene025378.t1
chr2.2 CDS 42315696 42316611 42315696 ID=cds.MS.gene025378.t1;Parent=MS.gene025378.t1
chr2.2 exon 42313896 42314584 42313896 ID=MS.gene025378.t1.exon2;Parent=MS.gene025378.t1
chr2.2 CDS 42313896 42314584 42313896 ID=cds.MS.gene025378.t1;Parent=MS.gene025378.t1
Gene Sequence

>MS.gene025378

ATGGCTTCCTCATCTCACAAACTCATCATCCTTCTCTTTACAATCCTCTTCTCACTCACATTACCTCTTCACACCAACACATCCACAACCTCATCATTCAATACCAACAAACTCAGTTCCTTAAAATCTTTATGCAAAACAACACCATACCCTAAACTTTGTTTCAACTCATTAAAACTTTCCATATCAATAAACATAAACCCAAACATCATAAACTTCCTTCTTCATTCACTTCAACTAGCAATATCCGAAACCACAAAGCTCTCTAATCTTTTCCACGACGTTGGAACATCGAATATCGTAGAGAAACAACGAGGATCAATTCAAGACTGCAAGGAACTTCATCAATCAACGTTAACTTCATTGAAAAGATCGTTATCCGGAATCCGTTCCTCGAACAAAAGAAACATAGCTGATGCTAGAATCTACCTAAGTGCAGCCCTAACCAACAAAAACACATGTTTAGATGGTCTTGATTCTGCTTCTGGTACATATAAACCAATTCTTGTGGATTCTATTATCAACACTTACAAACATGTAAGTAACTCTCTTTCTATGCTTTCTAATCATGCACCTGAGCCTTCAAATCAAAAGGGTTACAAGAAAAAATCTACTTCTCCAAAATGGTTATCAAAAAGCTTAGATTTGGATGAATATGATCCAAATGAAATGCTAGTTGTATCTGCAGATGGAAGTGGAAACTTTAGCACTATTAATGATGCTATAAACTTTGCTCCAAATAATAGTTTGGTTAGGATAGTTATTTATGTTAAAGAAGGGGTTTATGATGAAAATGTTGAAATACCAAGTTATAAAACTAACATTGTGATGCTTGGTGATGGAAGTGATTCTACTGTGATCACTGGTAATAGAAGTGTTGTTGATGGATGGACCACTTTCAGATCTGCAACTTTAGGTGAAGTTTTTCTTTACCCTTTTTTCTTGGTTTTGTCTTTTACTTCCTTGGATTTTTCTGTGCTTTGTCTTTTAAAAAAGAATAAGTGTTGCTGATGCAATAGATTCTACAATATTAAATTAAATTTGTCAATGAACTAATTATAGTGTGTTTGATTTCACAAGAGTTTTAAAATTGATTTTGTTTTGGATCTTATGTGATTTTAACAAAATATAGATGTTTTATGCTTAGGACAGAAATCATTTTGTTTCCCAATCTTTATTTGATGGTAAGTGGTAAAATTAATTTGTGTCTAGTTAAAAAAAATTGTCTTTGGAACATAAAATCAACATATAAATTTCTTCATTTCAGGCTAATTTGTTTTGGTCAAACATTATTTCAGGCTAACTTTATCCAAATCAATTTCAATGAAATTATTATTATTTTTTTTAAACTAATTCTTAAATTTTGGTTTAGGTTTTTCACAAGGTTGATCTCTTTTTAATGTGAGATTTGAGTTTTTCCAATATACCCTCTTTTCATATTATAATTTTATGGGTCATGTTAACTTGTGCCCTTAGAGCACAAGTTAAGGAACTTAAAATAGAAATAAGTAATAAATTTTATGTTGAAAATATAACTTTTGAAACTTTCAAGACATTAAATGCACAATTTCCAAGAAAAAACTTTTACTTTTGGTTCCTTAACTTATGCCCCAAGGTCACAAGTTAACATTTTCCTAATTTTATTTGGGGATTAACTCACCTATCTTGGATAGACACAAATACAATCTTAGAATTTGGGTTGTGTTCAGCGCAACGCTAAAGAATCAATTCGTAACACGAGAAATGTCATCCTTTATTAACCTTTTTCGGTTGTTATCTCTTTTCAATTTGGACTTGGATTTTTATCAATAATAATGTTCACAACTGGTTAAAAAAAAAAAAACTAAAGATTATGAGTAACTAATTTCATACTATGGATTGGTGACATTACAATAAAAGTTGGATTTTTGTCATTTGGGATAATCCATTGTTCAGTGAGTCAGATTCTTAATTTTTCCAAATAAATTAGTGTACCATGTTTCCAAATTGACAGTCACGTGATAATAAATGCTCATTTCATTTTTCAGCTGTATCTGGAGATGGATTTCTTGCAAGAAACATAGCATTTGAGAACAGAGCTGGACCAGAGAAACATCAAGCAGTTGCATTAAGAGTAAATGCAGATTTAACAGCATTTTACAAATGTGCAATTTATGGTTACCAGGACACACTTTATGTCCACTCATTTAGACAATTCTATCGCGAATGCGACATTTACGGGACAATAGATTTCATATTTGGTAACGCCGCAGTAGTATTACAAGAATGCGACATCGTATCCAGAATGCCATTACCTGGTCAATTTACTGTGATAACCGCGCAATCACGGGATAACCCTGACGAAGACACGGGAATCTCAATCCAAAACTGTTCGATTATCGCTACGGATGAGCTTTATTCAAACTCTAGTAAGGTCAAGAGTTACCTTGGGAGGCCATGGAGGGTTTTTTCTAGAACAGTTTTGATTGAGTCTTATATTGATGATTTTATTGATCAAAAGGGTTGGATAAAATGGTCAAATGATCAAGGTTTGGATACATTGTTTTATGGTGAATATGAAAATTATGGACCTGGTTCAAAGATTGATAATCGTGTTGAATGGGTTGGTTATCATTTGATGGATTATAATGATGCTTATAATTTCAGTGTTTCTGAGTTTATTATTGGTGATCAATGGCTTGAGTCTACTTCAGTTCCTTATGATGATGGAATTTGA
Protein sequence

>MS.gene025378.t1

MASSSHKLIILLFTILFSLTLPLHTNTSTTSSFNTNKLSSLKSLCKTTPYPKLCFNSLKLSISININPNIINFLLHSLQLAISETTKLSNLFHDVGTSNIVEKQRGSIQDCKELHQSTLTSLKRSLSGIRSSNKRNIADARIYLSAALTNKNTCLDGLDSASGTYKPILVDSIINTYKHVSNSLSMLSNHAPEPSNQKGYKKKSTSPKWLSKSLDLDEYDPNEMLVVSADGSGNFSTINDAINFAPNNSLVRIVIYVKEGVYDENVEIPSYKTNIVMLGDGSDSTVITGNRSVVDGWTTFRSATLAVSGDGFLARNIAFENRAGPEKHQAVALRVNADLTAFYKCAIYGYQDTLYVHSFRQFYRECDIYGTIDFIFGNAAVVLQECDIVSRMPLPGQFTVITAQSRDNPDEDTGISIQNCSIIATDELYSNSSKVKSYLGRPWRVFSRTVLIESYIDDFIDQKGWIKWSNDQGLDTLFYGEYENYGPGSKIDNRVEWVGYHLMDYNDAYNFSVSEFIIGDQWLESTSVPYDDGI