AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20749

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20749.t1 MTR_1g088020 96.403 556 20 0 1 556 1 556 0.0 1123
MS.gene20749.t1 MTR_1g088225 95.504 556 23 1 1 556 1 554 0.0 1081
MS.gene20749.t1 MTR_1g054880 87.701 561 62 2 1 556 1 559 0.0 1004
MS.gene20749.t1 MTR_0458s0010 82.194 556 62 4 1 556 1 519 0.0 891
MS.gene20749.t1 MTR_7g108890 75.752 532 126 2 26 556 34 563 0.0 852
MS.gene20749.t1 MTR_7g108875 66.604 533 173 3 26 556 36 565 0.0 747
MS.gene20749.t1 MTR_7g108900 66.163 529 172 5 26 551 32 556 0.0 728
MS.gene20749.t1 MTR_0458s0030 98.868 265 3 0 292 556 51 315 0.0 554
MS.gene20749.t1 MTR_0458s0030 82.353 51 9 0 1 51 1 51 3.27e-22 97.8
MS.gene20749.t1 MTR_1g088040 98.868 265 3 0 292 556 51 315 0.0 554
MS.gene20749.t1 MTR_1g088040 82.353 51 9 0 1 51 1 51 3.27e-22 97.8
MS.gene20749.t1 MTR_1g083520 50.730 548 236 9 12 555 11 528 0.0 525
MS.gene20749.t1 MTR_3g010770 47.610 544 260 9 32 556 39 576 2.73e-172 500
MS.gene20749.t1 MTR_8g042910 48.052 539 261 7 32 555 78 612 1.96e-171 499
MS.gene20749.t1 MTR_7g108905 51.546 485 194 10 77 551 99 552 1.15e-156 459
MS.gene20749.t1 MTR_4g025690 44.760 563 245 16 8 555 2 513 4.71e-141 418
MS.gene20749.t1 MTR_4g025730 45.763 531 227 15 32 555 33 509 2.97e-138 410
MS.gene20749.t1 MTR_4g124520 47.100 431 194 6 127 555 123 521 4.11e-134 400
MS.gene20749.t1 MTR_2g044810 41.021 568 279 11 5 555 6 534 4.23e-134 400
MS.gene20749.t1 MTR_8g023310 45.136 514 233 9 51 555 116 589 1.89e-133 401
MS.gene20749.t1 MTR_2g044880 45.030 493 220 11 73 555 74 525 6.58e-133 397
MS.gene20749.t1 MTR_1g008140 42.596 547 266 12 32 555 86 607 2.09e-132 399
MS.gene20749.t1 MTR_8g104655 42.780 554 281 12 8 555 18 541 2.50e-132 396
MS.gene20749.t1 MTR_4g087980 46.300 473 210 10 88 555 83 516 2.62e-132 395
MS.gene20749.t1 MTR_8g104645 56.024 332 142 3 225 555 44 372 1.61e-131 388
MS.gene20749.t1 MTR_7g112580 44.515 474 217 9 91 555 106 542 3.25e-131 394
MS.gene20749.t1 MTR_4g087970 44.658 468 223 9 91 555 83 517 2.70e-129 388
MS.gene20749.t1 MTR_8g042900 45.781 474 223 11 84 555 140 581 3.38e-129 390
MS.gene20749.t1 MTR_4g025670 44.654 477 216 8 83 555 57 489 1.47e-128 385
MS.gene20749.t1 MTR_3g010780 45.085 468 227 11 88 555 137 574 1.62e-128 388
MS.gene20749.t1 MTR_7g050870 44.609 473 215 10 91 555 121 554 3.74e-127 384
MS.gene20749.t1 MTR_7g050980 44.842 475 211 11 91 555 122 555 4.79e-127 383
MS.gene20749.t1 MTR_3g091380 42.115 539 274 11 32 555 82 597 8.49e-127 384
MS.gene20749.t1 MTR_7g050950 41.750 503 245 11 60 555 92 553 6.61e-123 372
MS.gene20749.t1 MTR_4g035980 40.260 539 268 13 32 555 40 539 1.95e-121 368
MS.gene20749.t1 MTR_3g033690 40.485 536 262 12 32 555 36 526 4.00e-120 365
MS.gene20749.t1 MTR_3g008720 39.479 537 267 13 32 555 36 527 2.93e-118 360
MS.gene20749.t1 MTR_3g008640 39.683 567 280 14 8 555 4 527 5.66e-118 359
MS.gene20749.t1 MTR_3g008700 39.474 532 274 10 32 555 36 527 8.90e-118 358
MS.gene20749.t1 MTR_7g053360 38.938 565 287 14 8 555 4 527 9.30e-117 356
MS.gene20749.t1 MTR_4g124550 41.277 470 249 7 91 555 146 593 8.07e-116 356
MS.gene20749.t1 MTR_1g086210 38.462 481 261 7 91 555 108 569 3.09e-108 335
MS.gene20749.t1 MTR_8g023150 38.290 538 288 15 29 555 78 582 1.61e-106 331
MS.gene20749.t1 MTR_2g044810 57.937 252 106 0 304 555 214 465 1.73e-106 327
MS.gene20749.t1 MTR_1g086190 36.174 528 319 8 31 555 51 563 4.08e-104 325
MS.gene20749.t1 MTR_7g112590 34.838 554 319 11 21 554 51 582 2.15e-102 321
MS.gene20749.t1 MTR_8g104580 37.726 554 276 15 8 555 10 500 4.97e-102 317
MS.gene20749.t1 MTR_1g083510 39.040 479 261 12 91 554 120 582 9.43e-102 319
MS.gene20749.t1 MTR_0618s0020 60.526 228 89 1 329 555 1 228 5.89e-100 302
MS.gene20749.t1 MTR_2g044660 50.160 313 149 3 243 555 327 632 3.37e-98 311
MS.gene20749.t1 MTR_1g083470 37.179 468 262 8 91 551 124 566 7.12e-97 306
MS.gene20749.t1 MTR_1g083500 37.179 468 262 8 91 551 124 566 7.12e-97 306
MS.gene20749.t1 MTR_1g083490 34.559 544 316 13 21 551 55 571 1.17e-95 303
MS.gene20749.t1 MTR_7g110560 33.818 550 326 13 17 556 29 550 1.28e-95 302
MS.gene20749.t1 MTR_4g005170 34.393 535 312 12 29 551 64 571 1.28e-94 302
MS.gene20749.t1 MTR_4g005180 34.249 546 315 13 21 551 37 553 2.91e-94 299
MS.gene20749.t1 MTR_8g093460 47.799 318 150 6 238 544 228 540 3.43e-94 298
MS.gene20749.t1 MTR_7g110490 37.317 477 273 8 88 550 105 569 3.29e-92 294
MS.gene20749.t1 MTR_7g110510 37.317 477 273 8 88 550 105 569 3.29e-92 294
MS.gene20749.t1 MTR_7g110440 36.326 479 279 8 91 555 108 574 7.47e-91 290
MS.gene20749.t1 MTR_4g120280 37.387 444 237 11 125 554 155 571 4.07e-90 289
MS.gene20749.t1 MTR_3g095920 35.340 515 271 13 62 554 89 563 4.51e-89 285
MS.gene20749.t1 MTR_4g120290 36.569 443 242 10 125 554 155 571 1.28e-87 282
MS.gene20749.t1 MTR_2g436040 32.458 533 318 10 29 552 44 543 3.30e-87 282
MS.gene20749.t1 MTR_5g083620 41.325 317 174 5 244 555 47 356 2.82e-80 256
MS.gene20749.t1 MTR_8g088610 40.483 331 183 6 225 552 54 373 3.35e-79 254
MS.gene20749.t1 MTR_2g436050 32.037 540 320 11 29 554 57 563 4.92e-78 256
MS.gene20749.t1 MTR_7g110420 33.809 491 287 10 91 555 108 586 1.48e-75 250
MS.gene20749.t1 MTR_7g107180 42.540 315 165 6 243 555 7 307 5.38e-75 240
MS.gene20749.t1 MTR_2g105560 40.000 315 171 6 243 555 41 339 4.70e-73 236
MS.gene20749.t1 MTR_7g110430 33.472 481 256 11 91 555 108 540 3.60e-72 240
MS.gene20749.t1 MTR_7g111560 29.520 542 317 13 30 554 62 555 5.10e-69 233
MS.gene20749.t1 MTR_1g086540 33.894 357 201 9 199 544 12 344 2.77e-53 185
MS.gene20749.t1 MTR_6g012800 35.258 329 179 10 242 553 5 316 7.17e-53 182
MS.gene20749.t1 MTR_6g024165 36.190 315 169 11 242 544 40 334 1.95e-51 179
MS.gene20749.t1 MTR_6g088810 35.738 305 168 7 251 544 90 377 1.60e-50 178
MS.gene20749.t1 MTR_7g011530 33.537 328 186 9 242 553 5 316 2.27e-49 173
MS.gene20749.t1 MTR_6g042660 34.653 303 162 8 221 518 25 296 3.78e-49 173
MS.gene20749.t1 MTR_8g102380 34.076 314 177 7 244 544 81 377 6.08e-49 174
MS.gene20749.t1 MTR_2g039530 39.677 310 146 11 225 523 40 319 2.88e-48 171
MS.gene20749.t1 MTR_7g092780 33.974 312 182 6 243 544 90 387 1.46e-47 171
MS.gene20749.t1 MTR_4g124740 32.588 313 175 9 242 544 53 339 1.14e-43 159
MS.gene20749.t1 MTR_1g033050 33.651 315 176 7 242 548 17 306 1.27e-43 158
MS.gene20749.t1 MTR_2g033970 32.372 312 182 7 243 545 56 347 5.18e-43 157
MS.gene20749.t1 MTR_7g102600 30.321 343 209 7 209 544 74 393 7.42e-43 158
MS.gene20749.t1 MTR_8g102380 35.878 262 139 7 244 492 81 326 1.05e-41 153
MS.gene20749.t1 MTR_5g012600 31.447 318 189 7 242 547 62 362 2.47e-41 153
MS.gene20749.t1 MTR_5g095490 32.792 308 178 7 249 544 67 357 3.70e-41 152
MS.gene20749.t1 MTR_5g083590 29.747 316 167 6 243 556 7 269 6.72e-41 149
MS.gene20749.t1 MTR_6g042690 32.803 314 175 10 243 544 39 328 2.16e-40 149
MS.gene20749.t1 MTR_5g093970 33.548 310 183 7 239 544 39 329 5.54e-40 148
MS.gene20749.t1 MTR_5g088300 33.007 306 183 7 239 544 33 316 5.08e-38 142
MS.gene20749.t1 MTR_2g436600 30.990 313 176 10 243 545 55 337 9.92e-38 142
MS.gene20749.t1 MTR_4g051810 31.392 309 191 6 238 546 32 319 1.28e-35 136
MS.gene20749.t1 MTR_5g088290 30.945 307 189 7 239 544 44 328 6.56e-35 134
MS.gene20749.t1 MTR_5g088280 30.945 307 188 7 239 544 35 318 2.95e-32 127
MS.gene20749.t1 MTR_8g036225 31.034 319 176 10 242 544 32 322 2.28e-31 124
MS.gene20749.t1 MTR_4g074290 29.355 310 198 7 240 544 68 361 1.10e-29 120
MS.gene20749.t1 MTR_2g073390 29.167 312 192 10 242 544 70 361 6.45e-28 115
MS.gene20749.t1 MTR_0009s0350 30.986 284 175 7 240 517 68 336 1.38e-27 114
MS.gene20749.t1 MTR_0009s0340 30.986 284 175 7 240 517 68 336 1.38e-27 114
MS.gene20749.t1 MTR_6g025590 36.702 188 100 7 223 400 33 211 8.14e-25 103
MS.gene20749.t1 MTR_0408s0030 35.878 131 70 4 415 544 31 148 9.78e-18 80.9
MS.gene20749.t1 MTR_4g130790 29.353 201 131 4 246 439 25 221 3.10e-15 76.3
MS.gene20749.t1 MTR_7g102580 44.186 86 38 2 320 400 73 153 7.17e-15 72.8
MS.gene20749.t1 MTR_6g024200 29.651 172 100 4 374 544 125 276 1.35e-14 74.7
MS.gene20749.t1 MTR_2g436070 25.664 339 195 13 29 343 57 362 2.98e-13 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20749.t1 AT1G02810 60.243 576 200 9 8 556 5 578 0.0 688
MS.gene20749.t1 AT4G02330 59.511 573 213 9 1 556 2 572 0.0 681
MS.gene20749.t1 AT2G47550 61.481 540 194 8 22 556 29 559 0.0 663
MS.gene20749.t1 AT3G10720 48.897 544 249 11 32 556 83 616 1.27e-173 505
MS.gene20749.t1 AT5G04970 45.287 541 273 10 32 556 88 621 3.14e-158 466
MS.gene20749.t1 AT2G26440 42.056 535 275 11 30 555 39 547 1.06e-137 410
MS.gene20749.t1 AT2G45220 42.806 563 261 13 1 555 2 511 1.64e-137 409
MS.gene20749.t1 AT3G43270 47.346 471 219 9 91 556 80 526 5.09e-132 395
MS.gene20749.t1 AT3G60730 47.696 434 213 9 126 555 96 519 1.02e-131 394
MS.gene20749.t1 AT1G53830 43.333 540 278 7 29 555 63 587 5.90e-130 392
MS.gene20749.t1 AT5G51490 41.912 544 251 11 32 555 38 536 7.16e-130 390
MS.gene20749.t1 AT4G33220 46.105 475 227 9 91 555 68 523 1.29e-129 389
MS.gene20749.t1 AT3G05620 43.659 481 212 8 91 555 104 541 1.47e-128 387
MS.gene20749.t1 AT1G23200 40.830 578 270 15 7 555 20 554 2.32e-128 387
MS.gene20749.t1 AT3G14310 42.413 547 275 10 32 555 63 592 4.31e-127 385
MS.gene20749.t1 AT1G11580 39.415 581 288 13 2 555 14 557 8.47e-127 383
MS.gene20749.t1 AT1G11580 39.415 581 288 13 2 555 35 578 2.45e-126 382
MS.gene20749.t1 AT3G10720 63.846 260 94 0 297 556 1 260 8.88e-126 369
MS.gene20749.t1 AT4G33220 55.162 339 142 3 218 555 73 402 1.26e-125 374
MS.gene20749.t1 AT4G02300 40.820 561 285 12 4 555 10 532 7.44e-124 374
MS.gene20749.t1 AT5G53370 46.882 433 206 10 127 555 173 585 2.77e-123 375
MS.gene20749.t1 AT5G53370 41.682 535 273 14 32 555 115 621 4.36e-123 375
MS.gene20749.t1 AT3G49220 46.119 438 210 9 123 555 180 596 1.17e-122 374
MS.gene20749.t1 AT3G49220 46.119 438 210 9 123 555 225 641 2.85e-122 374
MS.gene20749.t1 AT5G04960 40.719 501 254 11 57 553 101 562 5.45e-122 370
MS.gene20749.t1 AT2G26450 38.662 538 283 12 29 555 110 611 2.33e-119 365
MS.gene20749.t1 AT5G51500 39.962 533 277 11 32 555 42 540 3.39e-119 363
MS.gene20749.t1 AT3G10710 40.262 534 275 14 26 555 68 561 4.54e-119 363
MS.gene20749.t1 AT4G02320 41.754 479 248 9 81 555 67 518 2.88e-117 357
MS.gene20749.t1 AT3G05610 36.449 535 302 9 31 556 61 566 3.79e-115 356
MS.gene20749.t1 AT4G00190 39.179 536 247 14 31 555 7 474 4.86e-115 350
MS.gene20749.t1 AT1G53840 38.574 547 283 14 27 549 64 581 8.73e-114 350
MS.gene20749.t1 AT2G43050 42.128 470 225 8 88 556 94 517 1.14e-112 345
MS.gene20749.t1 AT3G59010 44.444 432 207 7 126 556 130 529 1.94e-111 342
MS.gene20749.t1 AT4G33230 36.330 545 295 12 29 555 96 606 8.97e-110 340
MS.gene20749.t1 AT5G49180 37.618 529 306 7 29 551 56 566 1.31e-106 331
MS.gene20749.t1 AT5G27870 38.004 471 257 6 91 555 121 562 1.40e-106 336
MS.gene20749.t1 AT3G47400 49.852 337 161 4 222 555 263 594 6.90e-104 325
MS.gene20749.t1 AT3G14300 41.064 470 239 10 91 550 525 966 1.85e-100 325
MS.gene20749.t1 AT5G20860 35.326 552 277 14 32 555 39 538 3.22e-100 313
MS.gene20749.t1 AT3G06830 40.000 430 243 6 126 553 150 566 5.30e-100 314
MS.gene20749.t1 AT4G03930 42.227 431 216 9 126 555 126 524 1.28e-99 311
MS.gene20749.t1 AT3G27980 41.259 429 211 8 130 555 107 497 1.54e-98 308
MS.gene20749.t1 AT1G11590 41.531 431 219 10 126 555 126 524 7.04e-97 305
MS.gene20749.t1 AT5G09760 47.152 316 142 8 243 543 241 546 1.61e-86 278
MS.gene20749.t1 AT5G09760 47.152 316 142 8 243 543 267 572 2.11e-86 279
MS.gene20749.t1 AT1G11370 53.279 244 111 1 243 486 46 286 7.70e-86 268
MS.gene20749.t1 AT4G15980 44.277 332 179 3 223 553 374 700 2.48e-85 279
MS.gene20749.t1 AT4G15980 44.277 332 179 3 223 553 384 710 4.37e-85 279
MS.gene20749.t1 AT5G64640 35.983 478 244 12 98 548 160 602 1.66e-82 269
MS.gene20749.t1 AT5G20860 32.468 539 256 13 32 542 39 497 9.51e-79 257
MS.gene20749.t1 AT2G47040 32.086 561 336 12 31 555 44 595 8.39e-75 249
MS.gene20749.t1 AT2G47030 43.175 315 172 4 244 555 278 588 9.32e-75 248
MS.gene20749.t1 AT3G62170 31.387 548 347 12 29 555 49 588 1.59e-71 240
MS.gene20749.t1 AT5G19730 35.987 314 173 7 242 544 85 381 6.11e-53 185
MS.gene20749.t1 AT3G29090 35.495 293 163 6 237 518 1 278 5.06e-50 175
MS.gene20749.t1 AT1G05310 35.897 312 176 6 243 544 91 388 4.65e-49 175
MS.gene20749.t1 AT2G21610 33.003 303 183 6 242 544 50 332 1.03e-48 172
MS.gene20749.t1 AT2G21610 33.003 303 183 6 242 544 55 337 1.23e-48 172
MS.gene20749.t1 AT2G36710 33.016 315 190 5 242 549 90 390 2.08e-48 173
MS.gene20749.t1 AT3G24130 35.093 322 174 8 243 550 34 334 5.80e-48 170
MS.gene20749.t1 AT5G18990 38.144 291 145 8 243 518 29 299 6.28e-48 170
MS.gene20749.t1 AT3G17060 31.731 312 184 7 243 545 46 337 9.78e-47 167
MS.gene20749.t1 AT2G36700 31.818 308 190 5 242 544 41 333 1.32e-46 166
MS.gene20749.t1 AT2G19150 30.341 323 195 8 239 551 37 339 1.36e-44 161
MS.gene20749.t1 AT5G07430 32.381 315 192 7 234 544 58 355 1.73e-41 153
MS.gene20749.t1 AT5G47500 31.111 315 188 7 242 544 60 357 6.26e-41 152
MS.gene20749.t1 AT5G55590 32.075 318 187 7 239 544 78 378 1.05e-39 149
MS.gene20749.t1 AT5G26810 32.229 332 195 8 225 544 20 333 1.62e-39 147
MS.gene20749.t1 AT2G47280 31.889 323 184 10 243 550 35 336 2.97e-39 146
MS.gene20749.t1 AT2G19150 32.824 262 148 7 239 490 37 280 9.46e-39 144
MS.gene20749.t1 AT5G55590 33.840 263 147 6 239 489 78 325 1.41e-37 142
MS.gene20749.t1 AT5G07420 30.744 309 189 8 242 544 66 355 9.60e-37 140
MS.gene20749.t1 AT1G44980 43.114 167 92 1 243 409 78 241 1.03e-35 134
MS.gene20749.t1 AT5G61680 31.392 309 188 9 242 544 68 358 1.04e-34 134
MS.gene20749.t1 AT1G69940 29.221 308 195 8 242 544 66 355 1.12e-33 132
MS.gene20749.t1 AT5G07410 29.221 308 195 8 242 544 66 355 1.76e-33 131

Find 111 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACAGTTGACTTCATATT+TGG 0.172980 1.2:+66068886 MS.gene20749:CDS
TCACTAGCAACCGTGTTATT+TGG 0.218847 1.2:-66068037 None:intergenic
GTAGAGCTGCAAATGGTTTC+TGG 0.229742 1.2:-66067329 None:intergenic
GAAATTGGACACCGTGAAAT+TGG 0.260395 1.2:-66069304 None:intergenic
ATCATCACCACTCAAGAAAT+TGG 0.269177 1.2:-66069319 None:intergenic
TTGTAGCTTTGAAGGGTATC+AGG 0.324620 1.2:+66068810 MS.gene20749:CDS
CTTTGTCTCAATCCACAATT+CGG 0.336867 1.2:+66067495 MS.gene20749:CDS
TATTAAAGCAGCAGATGATT+TGG 0.344745 1.2:+66069041 MS.gene20749:CDS
AATGGAAACATTTATGACTA+TGG 0.349973 1.2:+66067392 MS.gene20749:CDS
AACGTATTTGCCAATCAAAA+TGG 0.357020 1.2:+66067374 MS.gene20749:CDS
AGAACATCATGGCCACAAAA+TGG 0.357335 1.2:+66067809 MS.gene20749:CDS
GACTAACCAACAAACATGTT+TGG 0.374749 1.2:+66067655 MS.gene20749:CDS
TATATCAATCCACTAGAGTA+TGG 0.378755 1.2:-66069363 None:intergenic
GTTTGAACAGTTCCAACTTT+CGG 0.386865 1.2:-66069066 None:intergenic
TTCTATAGTTGTAGCTTTGA+AGG 0.390851 1.2:+66068802 MS.gene20749:CDS
TATTGAATTCATGAACTTAC+GGG 0.391914 1.2:-66067447 None:intergenic
ACCCTGCTGGTTGGCATGAA+TGG 0.394563 1.2:+66069160 MS.gene20749:CDS
GCAACTGCTTGGTGTTTGCT+TGG 0.397641 1.2:-66068751 None:intergenic
CACTTCTCATTGCAACTGCT+TGG 0.401773 1.2:-66068762 None:intergenic
GACCTTCCAAACATGTTTGT+TGG 0.404138 1.2:-66067661 None:intergenic
TATCGAGAATGCGATATATA+CGG 0.406490 1.2:+66068865 MS.gene20749:CDS
GTTGACTTCATATTTGGAAA+TGG 0.407911 1.2:+66068892 MS.gene20749:CDS
GGAGACCACAATGTTGTTGA+TGG 0.408728 1.2:+66068175 MS.gene20749:CDS
TTTGTCTCAATCCACAATTC+GGG 0.413019 1.2:+66067496 MS.gene20749:CDS
ACATTCAATTCAGCCACATT+TGG 0.413259 1.2:+66068205 MS.gene20749:CDS
GCTTTATTTGACATCTTCAA+TGG 0.419717 1.2:-66067863 None:intergenic
AGATTGATTTGTTGTTGTAT+AGG 0.420233 1.2:-66067573 None:intergenic
CTATTGAATTCATGAACTTA+CGG 0.421755 1.2:-66067448 None:intergenic
AAGTCTTCTGCTTGAGAAGT+TGG 0.427453 1.2:-66067608 None:intergenic
ATTTCTTGAGTGGTGATGAT+TGG 0.428264 1.2:+66069322 MS.gene20749:CDS
GAACAAGATCGAAAGTAGAT+AGG 0.433031 1.2:-66067273 None:intergenic
TTCAAAGCTACAACTATAGA+AGG 0.433228 1.2:-66068800 None:intergenic
TTCCATTCATGCCAACCAGC+AGG 0.438837 1.2:-66069162 None:intergenic
ATTCTTTCCACGGTCTTCCT+AGG 0.445831 1.2:-66069092 None:intergenic
CATTGTATACACATTCTCTA+AGG 0.446591 1.2:+66068836 MS.gene20749:CDS
TTTACAATAAGTAGGGTTTA+CGG 0.448955 1.2:-66067351 None:intergenic
TGGCCTGGTTATCATGTTAT+CGG 0.452691 1.2:+66069267 MS.gene20749:CDS
ATTGTCGTTGTTAGTCAAGA+TGG 0.461728 1.2:+66067971 MS.gene20749:CDS
GTATCATTCCATGTAGCAAA+TGG 0.462739 1.2:+66067299 MS.gene20749:CDS
TTAAATTTGCTTACCAAATG+TGG 0.464595 1.2:-66068218 None:intergenic
ATACTCTTGATACACACCTT+TGG 0.465883 1.2:-66068083 None:intergenic
ACCCTAGCCTTGTTCAAGAA+AGG 0.466853 1.2:+66067764 MS.gene20749:CDS
GAACTTACGGGATTGCGATA+AGG 0.466990 1.2:-66067435 None:intergenic
GATGATTTGGCTCCGAAAGT+TGG 0.467758 1.2:+66069054 MS.gene20749:CDS
AACTCAATTCAGCAAGGAAT+CGG 0.469791 1.2:-66067532 None:intergenic
AGCCTTGTTCAAGAAAGGTT+GGG 0.473203 1.2:+66067769 MS.gene20749:CDS
TCAATAGACTCACAACTTCA+AGG 0.474105 1.2:+66067464 MS.gene20749:CDS
TAGCCTTGTTCAAGAAAGGT+TGG 0.480777 1.2:+66067768 MS.gene20749:CDS
TACCGATGCAGCCAATTTCA+CGG 0.481016 1.2:+66069293 MS.gene20749:CDS
TACGAACCCTTGACCAACTA+CGG 0.486181 1.2:-66068695 None:intergenic
GTGGAACACCATTTGCTACA+TGG 0.489190 1.2:-66067307 None:intergenic
ATCAACAACATTGTGGTCTC+CGG 0.489800 1.2:-66068173 None:intergenic
AACCTTTCTTGAACAAGGCT+AGG 0.499650 1.2:-66067766 None:intergenic
CACCGTGAAATTGGCTGCAT+CGG 0.500180 1.2:-66069295 None:intergenic
ATGATTCTGCTAGACGCAAT+GGG 0.504675 1.2:+66067897 MS.gene20749:CDS
AGCTACTGCGTCATTGATAG+TGG 0.505172 1.2:-66068008 None:intergenic
TGTTTGTTGGTTAGTCAAAA+CGG 0.505795 1.2:-66067648 None:intergenic
GACCGTGGAAAGAATATTCA+AGG 0.508246 1.2:+66069100 MS.gene20749:CDS
TATCCTCGCCTTCCGCTAAG+TGG 0.509384 1.2:+66068943 MS.gene20749:CDS
TTCTTCATATTTATCACCAA+AGG 0.513617 1.2:+66068067 MS.gene20749:CDS
TATGATTCTGCTAGACGCAA+TGG 0.514899 1.2:+66067896 MS.gene20749:CDS
GTCCTTGAATATTCTTTCCA+CGG 0.519966 1.2:-66069102 None:intergenic
GACAGTTTCATAAACCCTGC+TGG 0.520411 1.2:+66069147 MS.gene20749:CDS
TCTATAGTTGTAGCTTTGAA+GGG 0.523876 1.2:+66068803 MS.gene20749:CDS
ATAACATTTCGCAACACCGC+TGG 0.525873 1.2:+66068727 MS.gene20749:CDS
ACTTTGTACTATGCAGAATA+CGG 0.531445 1.2:+66069204 MS.gene20749:CDS
AGGCCATGTCACTCGATTCA+CGG 0.531644 1.2:-66069250 None:intergenic
AGTTCGACCTTGAGCAGTGA+TGG 0.533091 1.2:-66068977 None:intergenic
AATAACACGGTTGCTAGTGA+TGG 0.541511 1.2:+66068040 MS.gene20749:CDS
GAATACGGCAACAGAGGAGC+GGG 0.544190 1.2:+66069219 MS.gene20749:CDS
GTTTACGGTAGAGCTGCAAA+TGG 0.544890 1.2:-66067336 None:intergenic
CAAGCAGTTGCAATGAGAAG+TGG 0.548536 1.2:+66068763 MS.gene20749:CDS
TACTTGATGATGGTTGGAGA+GGG 0.549006 1.2:+66068130 MS.gene20749:CDS
AACCAACAAACATGTTTGGA+AGG 0.550080 1.2:+66067659 MS.gene20749:CDS
GAGTATGGAACACCAGTTTG+AGG 0.551293 1.2:-66069348 None:intergenic
GATGATTGGATTCCTCAAAC+TGG 0.552199 1.2:+66069336 MS.gene20749:CDS
TTCAATTCCATCACTGCTCA+AGG 0.554113 1.2:+66068970 MS.gene20749:CDS
ACTGGTGTTCCATACTCTAG+TGG 0.555255 1.2:+66069354 MS.gene20749:CDS
ACGGTGTCCAATTTCTTGAG+TGG 0.555293 1.2:+66069312 MS.gene20749:CDS
ACCTTTCTTGAACAAGGCTA+GGG 0.557941 1.2:-66067765 None:intergenic
GAATTGAATTGTCCACTTAG+CGG 0.559760 1.2:-66068955 None:intergenic
GTTGTTAGTCAAGATGGAAG+TGG 0.562167 1.2:+66067977 MS.gene20749:CDS
ATCTTCAAGAGCCCGAATTG+TGG 0.568143 1.2:-66067507 None:intergenic
GTGAATCGAGTGACATGGCC+TGG 0.573051 1.2:+66069252 MS.gene20749:CDS
AAAGTAAAATAAGAACATCA+TGG 0.577883 1.2:+66067798 MS.gene20749:CDS
ATCAATCAAACAGTTATCAC+CGG 0.578637 1.2:+66068154 MS.gene20749:CDS
ACTGATCCAAATCAAAACAC+TGG 0.582550 1.2:+66068997 MS.gene20749:CDS
GTTTCATAAACCCTGCTGGT+TGG 0.584669 1.2:+66069151 MS.gene20749:CDS
TCCATTCATGCCAACCAGCA+GGG 0.585336 1.2:-66069161 None:intergenic
TTTCAGCCGTAGTTGGTCAA+GGG 0.587591 1.2:+66068689 MS.gene20749:intron
TTGGTGTTTGCTTGGTCCAG+CGG 0.591249 1.2:-66068743 None:intergenic
AACCCAACCTTTCTTGAACA+AGG 0.595250 1.2:-66067771 None:intergenic
GTACTTGATGATGGTTGGAG+AGG 0.601393 1.2:+66068129 MS.gene20749:CDS
TGAATTGTCCACTTAGCGGA+AGG 0.605583 1.2:-66068951 None:intergenic
GGAACTGTTCAAACATACCT+AGG 0.606114 1.2:+66069075 MS.gene20749:CDS
GAGCTGCAAATGGTTTCTGG+TGG 0.615495 1.2:-66067326 None:intergenic
CTATAGCTTCTGATCAGAGA+GGG 0.616495 1.2:+66067690 MS.gene20749:CDS
TGTCCACTTAGCGGAAGGCG+AGG 0.623788 1.2:-66068946 None:intergenic
ATTCAAAACTCAATTCAGCA+AGG 0.638769 1.2:-66067538 None:intergenic
GCTGGTTGGCATGAATGGAA+TGG 0.651490 1.2:+66069165 MS.gene20749:CDS
GCACCGTGAATCGAGTGACA+TGG 0.652496 1.2:+66069247 MS.gene20749:CDS
GCACCGATAACATGATAACC+AGG 0.655111 1.2:-66069270 None:intergenic
TATGCAGAATACGGCAACAG+AGG 0.658760 1.2:+66069213 MS.gene20749:CDS
AGAATACGGCAACAGAGGAG+CGG 0.660300 1.2:+66069218 MS.gene20749:CDS
AGCTGCTGCACCAAATAACA+CGG 0.675314 1.2:+66068027 MS.gene20749:CDS
TGAAACCATCAACAACATTG+TGG 0.688631 1.2:-66068180 None:intergenic
TACTAATGAAGATAGTGTTG+TGG 0.688812 1.2:+66067940 MS.gene20749:CDS
ACTATAGCTTCTGATCAGAG+AGG 0.691912 1.2:+66067689 MS.gene20749:CDS
AAACATACCTAGGAAGACCG+TGG 0.695141 1.2:+66069085 MS.gene20749:CDS
AAAGCTACAACTATAGAAGG+CGG 0.706210 1.2:-66068797 None:intergenic
ATAGACTCACAACTTCAAGG+AGG 0.766722 1.2:+66067467 MS.gene20749:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTATATTGTGATTTA+TGG + chr1.2:66068608-66068627 MS.gene20749:intron 10.0%
!! TCAAATATACGTTTAAAAAT+CGG - chr1.2:66068467-66068486 None:intergenic 15.0%
!! AAAGTAAAATAAGAACATCA+TGG + chr1.2:66067798-66067817 MS.gene20749:CDS 20.0%
!! AAGCATTTGAATTTCAAAAA+TGG + chr1.2:66067833-66067852 MS.gene20749:CDS 20.0%
!! ATCATTTCTCTAACAAAAAA+TGG + chr1.2:66068435-66068454 MS.gene20749:intron 20.0%
!! TTAAACGTATATTTGATGAA+TGG + chr1.2:66068471-66068490 MS.gene20749:intron 20.0%
!!! AATACGTTTTTACAATAAGT+AGG - chr1.2:66067362-66067381 None:intergenic 20.0%
!!! AATGATATTTTGACAACTTT+TGG - chr1.2:66068421-66068440 None:intergenic 20.0%
!!! ATAAATGTTTCCATTTTGAT+TGG - chr1.2:66067387-66067406 None:intergenic 20.0%
!!! ATACGTTTTTACAATAAGTA+GGG - chr1.2:66067361-66067380 None:intergenic 20.0%
!!! ATCAAGTACTTTTTGTTTTT+GGG - chr1.2:66068118-66068137 None:intergenic 20.0%
!!! GATGTTCTTATTTTACTTTT+AGG - chr1.2:66067797-66067816 None:intergenic 20.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr1.2:66068324-66068343 None:intergenic 25.0%
! AATGGAAACATTTATGACTA+TGG + chr1.2:66067392-66067411 MS.gene20749:CDS 25.0%
! CTATTGAATTCATGAACTTA+CGG - chr1.2:66067451-66067470 None:intergenic 25.0%
! TATTGAATTCATGAACTTAC+GGG - chr1.2:66067450-66067469 None:intergenic 25.0%
! TGGTAAGCAAATTTAATACT+TGG + chr1.2:66068225-66068244 MS.gene20749:intron 25.0%
! TTAGCAATCATTGTAAAAGT+CGG - chr1.2:66068532-66068551 None:intergenic 25.0%
! TTCTTATTGGTCAAAAACAA+TGG + chr1.2:66068336-66068355 MS.gene20749:intron 25.0%
! TTCTTCATATTTATCACCAA+AGG + chr1.2:66068067-66068086 MS.gene20749:CDS 25.0%
! TTTACAATAAGTAGGGTTTA+CGG - chr1.2:66067354-66067373 None:intergenic 25.0%
!! AGTTTTTATGCAATCTTTCA+TGG + chr1.2:66069125-66069144 MS.gene20749:CDS 25.0%
!! CTTTTACAATGATTGCTAAA+AGG + chr1.2:66068533-66068552 MS.gene20749:intron 25.0%
!! GTTACAATTTTGCAAAACAA+CGG - chr1.2:66068920-66068939 None:intergenic 25.0%
!! TTAAATTTGCTTACCAAATG+TGG - chr1.2:66068221-66068240 None:intergenic 25.0%
!!! AAACAAAAAGTACTTGATGA+TGG + chr1.2:66068120-66068139 MS.gene20749:CDS 25.0%
!!! AGATTGATTTGTTGTTGTAT+AGG - chr1.2:66067576-66067595 None:intergenic 25.0%
!!! CATCAAGTACTTTTTGTTTT+TGG - chr1.2:66068119-66068138 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr1.2:66068323-66068342 MS.gene20749:intron 25.0%
AAAACAATGGAGAGAGAAAA+AGG + chr1.2:66068349-66068368 MS.gene20749:intron 30.0%
AACGTATTTGCCAATCAAAA+TGG + chr1.2:66067374-66067393 MS.gene20749:CDS 30.0%
ACTTTGTACTATGCAGAATA+CGG + chr1.2:66069204-66069223 MS.gene20749:CDS 30.0%
ATCAATCAAACAGTTATCAC+CGG + chr1.2:66068154-66068173 MS.gene20749:CDS 30.0%
ATTCAAAACTCAATTCAGCA+AGG - chr1.2:66067541-66067560 None:intergenic 30.0%
CATTGTATACACATTCTCTA+AGG + chr1.2:66068836-66068855 MS.gene20749:CDS 30.0%
TACTAATGAAGATAGTGTTG+TGG + chr1.2:66067940-66067959 MS.gene20749:CDS 30.0%
TATATCAATCCACTAGAGTA+TGG - chr1.2:66069366-66069385 None:intergenic 30.0%
TATCGAGAATGCGATATATA+CGG + chr1.2:66068865-66068884 MS.gene20749:CDS 30.0%
TATTAAAGCAGCAGATGATT+TGG + chr1.2:66069041-66069060 MS.gene20749:CDS 30.0%
TCAACAAAGTTGTCATCAAA+TGG - chr1.2:66068298-66068317 None:intergenic 30.0%
TTCAAAGCTACAACTATAGA+AGG - chr1.2:66068803-66068822 None:intergenic 30.0%
! GTTGACTTCATATTTGGAAA+TGG + chr1.2:66068892-66068911 MS.gene20749:CDS 30.0%
! TGTTTGTTGGTTAGTCAAAA+CGG - chr1.2:66067651-66067670 None:intergenic 30.0%
!! AAAAAGTACTTGATGATGGT+TGG + chr1.2:66068124-66068143 MS.gene20749:CDS 30.0%
!! GCTTTATTTGACATCTTCAA+TGG - chr1.2:66067866-66067885 None:intergenic 30.0%
!! TCTATAGTTGTAGCTTTGAA+GGG + chr1.2:66068803-66068822 MS.gene20749:CDS 30.0%
!! TTCTATAGTTGTAGCTTTGA+AGG + chr1.2:66068802-66068821 MS.gene20749:CDS 30.0%
AAAGCTACAACTATAGAAGG+CGG - chr1.2:66068800-66068819 None:intergenic 35.0%
AACCAACAAACATGTTTGGA+AGG + chr1.2:66067659-66067678 MS.gene20749:CDS 35.0%
AACTCAATTCAGCAAGGAAT+CGG - chr1.2:66067535-66067554 None:intergenic 35.0%
ACATTCAATTCAGCCACATT+TGG + chr1.2:66068205-66068224 MS.gene20749:CDS 35.0%
ACTGATCCAAATCAAAACAC+TGG + chr1.2:66068997-66069016 MS.gene20749:CDS 35.0%
ATACTCTTGATACACACCTT+TGG - chr1.2:66068086-66068105 None:intergenic 35.0%
ATCATCACCACTCAAGAAAT+TGG - chr1.2:66069322-66069341 None:intergenic 35.0%
ATTGTCGTTGTTAGTCAAGA+TGG + chr1.2:66067971-66067990 MS.gene20749:CDS 35.0%
CTTTGTCTCAATCCACAATT+CGG + chr1.2:66067495-66067514 MS.gene20749:CDS 35.0%
GAACAAGATCGAAAGTAGAT+AGG - chr1.2:66067276-66067295 None:intergenic 35.0%
GAATTGAATTGTCCACTTAG+CGG - chr1.2:66068958-66068977 None:intergenic 35.0%
GACTAACCAACAAACATGTT+TGG + chr1.2:66067655-66067674 MS.gene20749:CDS 35.0%
GTATCATTCCATGTAGCAAA+TGG + chr1.2:66067299-66067318 MS.gene20749:CDS 35.0%
GTCCTTGAATATTCTTTCCA+CGG - chr1.2:66069105-66069124 None:intergenic 35.0%
GTTTGAACAGTTCCAACTTT+CGG - chr1.2:66069069-66069088 None:intergenic 35.0%
TCAATAGACTCACAACTTCA+AGG + chr1.2:66067464-66067483 MS.gene20749:CDS 35.0%
TGAAACCATCAACAACATTG+TGG - chr1.2:66068183-66068202 None:intergenic 35.0%
TTTGTCTCAATCCACAATTC+GGG + chr1.2:66067496-66067515 MS.gene20749:CDS 35.0%
! ATTTCTTGAGTGGTGATGAT+TGG + chr1.2:66069322-66069341 MS.gene20749:CDS 35.0%
! GGAACAGTTGACTTCATATT+TGG + chr1.2:66068886-66068905 MS.gene20749:CDS 35.0%
!!! GAAGTTCCAGTGTTTTGATT+TGG - chr1.2:66069006-66069025 None:intergenic 35.0%
!!! TTCAAATGCTTGCCATTTTG+TGG - chr1.2:66067824-66067843 None:intergenic 35.0%
AACCCAACCTTTCTTGAACA+AGG - chr1.2:66067774-66067793 None:intergenic 40.0%
AACCTTTCTTGAACAAGGCT+AGG - chr1.2:66067769-66067788 None:intergenic 40.0%
AAGTCTTCTGCTTGAGAAGT+TGG - chr1.2:66067611-66067630 None:intergenic 40.0%
AATAACACGGTTGCTAGTGA+TGG + chr1.2:66068040-66068059 MS.gene20749:CDS 40.0%
ACCTTTCTTGAACAAGGCTA+GGG - chr1.2:66067768-66067787 None:intergenic 40.0%
ACTATAGCTTCTGATCAGAG+AGG + chr1.2:66067689-66067708 MS.gene20749:CDS 40.0%
AGAACATCATGGCCACAAAA+TGG + chr1.2:66067809-66067828 MS.gene20749:CDS 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr1.2:66068359-66068378 MS.gene20749:intron 40.0%
ATAGACTCACAACTTCAAGG+AGG + chr1.2:66067467-66067486 MS.gene20749:CDS 40.0%
ATCAACAACATTGTGGTCTC+CGG - chr1.2:66068176-66068195 None:intergenic 40.0%
ATGATTCTGCTAGACGCAAT+GGG + chr1.2:66067897-66067916 MS.gene20749:CDS 40.0%
CTATAGCTTCTGATCAGAGA+GGG + chr1.2:66067690-66067709 MS.gene20749:CDS 40.0%
GAAATTGGACACCGTGAAAT+TGG - chr1.2:66069307-66069326 None:intergenic 40.0%
GACCGTGGAAAGAATATTCA+AGG + chr1.2:66069100-66069119 MS.gene20749:CDS 40.0%
GACCTTCCAAACATGTTTGT+TGG - chr1.2:66067664-66067683 None:intergenic 40.0%
GATGATTGGATTCCTCAAAC+TGG + chr1.2:66069336-66069355 MS.gene20749:CDS 40.0%
GGAACTGTTCAAACATACCT+AGG + chr1.2:66069075-66069094 MS.gene20749:CDS 40.0%
GTTGTTAGTCAAGATGGAAG+TGG + chr1.2:66067977-66067996 MS.gene20749:CDS 40.0%
TATGATTCTGCTAGACGCAA+TGG + chr1.2:66067896-66067915 MS.gene20749:CDS 40.0%
TGGCCTGGTTATCATGTTAT+CGG + chr1.2:66069267-66069286 MS.gene20749:CDS 40.0%
TTCAATTCCATCACTGCTCA+AGG + chr1.2:66068970-66068989 MS.gene20749:CDS 40.0%
! TCACTAGCAACCGTGTTATT+TGG - chr1.2:66068040-66068059 None:intergenic 40.0%
! TGTGAATTTTCAGCCGTAGT+TGG + chr1.2:66068682-66068701 MS.gene20749:intron 40.0%
!! AGCCTTGTTCAAGAAAGGTT+GGG + chr1.2:66067769-66067788 MS.gene20749:CDS 40.0%
!! TACTTGATGATGGTTGGAGA+GGG + chr1.2:66068130-66068149 MS.gene20749:CDS 40.0%
!! TAGCCTTGTTCAAGAAAGGT+TGG + chr1.2:66067768-66067787 MS.gene20749:CDS 40.0%
!! TTGTAGCTTTGAAGGGTATC+AGG + chr1.2:66068810-66068829 MS.gene20749:CDS 40.0%
AAACATACCTAGGAAGACCG+TGG + chr1.2:66069085-66069104 MS.gene20749:CDS 45.0%
ACGGTGTCCAATTTCTTGAG+TGG + chr1.2:66069312-66069331 MS.gene20749:CDS 45.0%
ACTGGTGTTCCATACTCTAG+TGG + chr1.2:66069354-66069373 MS.gene20749:CDS 45.0%
AGCTACTGCGTCATTGATAG+TGG - chr1.2:66068011-66068030 None:intergenic 45.0%
AGCTGCTGCACCAAATAACA+CGG + chr1.2:66068027-66068046 MS.gene20749:CDS 45.0%
ATAACATTTCGCAACACCGC+TGG + chr1.2:66068727-66068746 MS.gene20749:CDS 45.0%
ATCTTCAAGAGCCCGAATTG+TGG - chr1.2:66067510-66067529 None:intergenic 45.0%
ATTCTTTCCACGGTCTTCCT+AGG - chr1.2:66069095-66069114 None:intergenic 45.0%
CAAGCAGTTGCAATGAGAAG+TGG + chr1.2:66068763-66068782 MS.gene20749:CDS 45.0%
CACTTCTCATTGCAACTGCT+TGG - chr1.2:66068765-66068784 None:intergenic 45.0%
GAACTTACGGGATTGCGATA+AGG - chr1.2:66067438-66067457 None:intergenic 45.0%
GACAGTTTCATAAACCCTGC+TGG + chr1.2:66069147-66069166 MS.gene20749:CDS 45.0%
GAGTATGGAACACCAGTTTG+AGG - chr1.2:66069351-66069370 None:intergenic 45.0%
GGAGACCACAATGTTGTTGA+TGG + chr1.2:66068175-66068194 MS.gene20749:CDS 45.0%
GTAGAGCTGCAAATGGTTTC+TGG - chr1.2:66067332-66067351 None:intergenic 45.0%
GTTTACGGTAGAGCTGCAAA+TGG - chr1.2:66067339-66067358 None:intergenic 45.0%
GTTTCATAAACCCTGCTGGT+TGG + chr1.2:66069151-66069170 MS.gene20749:CDS 45.0%
TACCGATGCAGCCAATTTCA+CGG + chr1.2:66069293-66069312 MS.gene20749:CDS 45.0%
TACGAACCCTTGACCAACTA+CGG - chr1.2:66068698-66068717 None:intergenic 45.0%
TATGCAGAATACGGCAACAG+AGG + chr1.2:66069213-66069232 MS.gene20749:CDS 45.0%
TGAATTGTCCACTTAGCGGA+AGG - chr1.2:66068954-66068973 None:intergenic 45.0%
TTTCAGCCGTAGTTGGTCAA+GGG + chr1.2:66068689-66068708 MS.gene20749:intron 45.0%
! GATGATTTGGCTCCGAAAGT+TGG + chr1.2:66069054-66069073 MS.gene20749:CDS 45.0%
! GCACCGATAACATGATAACC+AGG - chr1.2:66069273-66069292 None:intergenic 45.0%
! GTGGAACACCATTTGCTACA+TGG - chr1.2:66067310-66067329 None:intergenic 45.0%
! TTTTCAGCCGTAGTTGGTCA+AGG + chr1.2:66068688-66068707 MS.gene20749:intron 45.0%
!! ACCCTAGCCTTGTTCAAGAA+AGG + chr1.2:66067764-66067783 MS.gene20749:CDS 45.0%
!! GTACTTGATGATGGTTGGAG+AGG + chr1.2:66068129-66068148 MS.gene20749:CDS 45.0%
AGAATACGGCAACAGAGGAG+CGG + chr1.2:66069218-66069237 MS.gene20749:CDS 50.0%
AGGCCATGTCACTCGATTCA+CGG - chr1.2:66069253-66069272 None:intergenic 50.0%
AGTTCGACCTTGAGCAGTGA+TGG - chr1.2:66068980-66068999 None:intergenic 50.0%
CACCGTGAAATTGGCTGCAT+CGG - chr1.2:66069298-66069317 None:intergenic 50.0%
GCTGGTTGGCATGAATGGAA+TGG + chr1.2:66069165-66069184 MS.gene20749:CDS 50.0%
TCCATTCATGCCAACCAGCA+GGG - chr1.2:66069164-66069183 None:intergenic 50.0%
TTCCATTCATGCCAACCAGC+AGG - chr1.2:66069165-66069184 None:intergenic 50.0%
! GAGCTGCAAATGGTTTCTGG+TGG - chr1.2:66067329-66067348 None:intergenic 50.0%
! TTGGTGTTTGCTTGGTCCAG+CGG - chr1.2:66068746-66068765 None:intergenic 50.0%
!! GCAACTGCTTGGTGTTTGCT+TGG - chr1.2:66068754-66068773 None:intergenic 50.0%
GAATACGGCAACAGAGGAGC+GGG + chr1.2:66069219-66069238 MS.gene20749:CDS 55.0%
GCACCGTGAATCGAGTGACA+TGG + chr1.2:66069247-66069266 MS.gene20749:CDS 55.0%
GTGAATCGAGTGACATGGCC+TGG + chr1.2:66069252-66069271 MS.gene20749:CDS 55.0%
TATCCTCGCCTTCCGCTAAG+TGG + chr1.2:66068943-66068962 MS.gene20749:CDS 55.0%
! ACCCTGCTGGTTGGCATGAA+TGG + chr1.2:66069160-66069179 MS.gene20749:CDS 55.0%
TGTCCACTTAGCGGAAGGCG+AGG - chr1.2:66068949-66068968 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 66067248 66069386 66067248 ID=MS.gene20749
chr1.2 mRNA 66067248 66069386 66067248 ID=MS.gene20749.t1;Parent=MS.gene20749
chr1.2 exon 66067248 66068226 66067248 ID=MS.gene20749.t1.exon1;Parent=MS.gene20749.t1
chr1.2 CDS 66067248 66068226 66067248 ID=cds.MS.gene20749.t1;Parent=MS.gene20749.t1
chr1.2 exon 66068695 66069386 66068695 ID=MS.gene20749.t1.exon2;Parent=MS.gene20749.t1
chr1.2 CDS 66068695 66069386 66068695 ID=cds.MS.gene20749.t1;Parent=MS.gene20749.t1
Gene Sequence

>MS.gene20749

ATGGCTCTCAAACACAAAACTATTGCCTATCTACTTTCGATCTTGTTCTTTGTATCATTCCATGTAGCAAATGGTGTTCCACCAGAAACCATTTGCAGCTCTACCGTAAACCCTACTTATTGTAAAAACGTATTTGCCAATCAAAATGGAAACATTTATGACTATGGTAAAATTTCAATTCGAAAATCCTTATCGCAATCCCGTAAGTTCATGAATTCAATAGACTCACAACTTCAAGGAGGTTCATCTTTGTCTCAATCCACAATTCGGGCTCTTGAAGATTGCCGATTCCTTGCTGAATTGAGTTTTGAATACTTATCAAACACCTATACAACAACAAATCAATCTAGTAATGTTCTTCCAACTTCTCAAGCAGAAGACTTTGAAACTTTTCTAAGTGCCGTTTTGACTAACCAACAAACATGTTTGGAAGGTCTAAACACTATAGCTTCTGATCAGAGAGGGAAGAACGACTTAACGTCATCGATCTCTGATGACATGAAGCTTCATAGTGTCACCCTAGCCTTGTTCAAGAAAGGTTGGGTTCCTAAAAGTAAAATAAGAACATCATGGCCACAAAATGGCAAGCATTTGAATTTCAAAAATGGTCGTTTGCCATTGAAGATGTCAAATAAAGCACGTGCTATTTATGATTCTGCTAGACGCAATGGGAGAAAACTACTTCAAACTAATACTAATGAAGATAGTGTTGTGGTGAGTGATATTGTCGTTGTTAGTCAAGATGGAAGTGGAAACTTTACCACTATCAATGACGCAGTAGCTGCTGCACCAAATAACACGGTTGCTAGTGATGGATACTTCTTCATATTTATCACCAAAGGTGTGTATCAAGAGTATGTATCTATACCCAAAAACAAAAAGTACTTGATGATGGTTGGAGAGGGAATCAATCAAACAGTTATCACCGGAGACCACAATGTTGTTGATGGTTTCACAACATTCAATTCAGCCACATTTGGTAAGCAAATTTAATACTTGGACATATTAAATATACTATTAACAAAAAGAGTAATGATATTTTGACATCCATTTGATGACAACTTTGTTGACAACCTTGTTTTTCTCACTTCTTATTGGTCAAAAACAATGGAGAGAGAAAAAGGAAGAGAGAGGATAACAACATCATGTGAGTATGAGAGAGAAAGTTGTCCAAAAGTTGTCAAAATATCATTTCTCTAACAAAAAATGGTATATACCGATTTTTAAACGTATATTTGATGAATGGTTTGAAAATTTTGTCGCTAATGTGATTGAAAATTTCCGACTTTTACAATGATTGCTAAAAGGCAATTTTTTTATTTTTTTTTAGTTTACAATTACTCACACAAATAGTGTTAATATATTTTATATTGTGATTTATGGCATGCATGAAGATAATCTATATGTTAAAAATTTCACAAATTTGACGTTTCTTGTGAATTTTCAGCCGTAGTTGGTCAAGGGTTCGTAGCAGTTAACATAACATTTCGCAACACCGCTGGACCAAGCAAACACCAAGCAGTTGCAATGAGAAGTGGAGCAGATATGTCCGCCTTCTATAGTTGTAGCTTTGAAGGGTATCAGGACACATTGTATACACATTCTCTAAGGCAGTTTTATCGAGAATGCGATATATACGGAACAGTTGACTTCATATTTGGAAATGGTGCCGTTGTTTTGCAAAATTGTAACATTTATCCTCGCCTTCCGCTAAGTGGACAATTCAATTCCATCACTGCTCAAGGTCGAACTGATCCAAATCAAAACACTGGAACTTCTATACAAAATGCAACTATTAAAGCAGCAGATGATTTGGCTCCGAAAGTTGGAACTGTTCAAACATACCTAGGAAGACCGTGGAAAGAATATTCAAGGACAGTTTTTATGCAATCTTTCATGGACAGTTTCATAAACCCTGCTGGTTGGCATGAATGGAATGGTGATTTTGCATTGAATACTTTGTACTATGCAGAATACGGCAACAGAGGAGCGGGTTCAAGCACCGTGAATCGAGTGACATGGCCTGGTTATCATGTTATCGGTGCTACCGATGCAGCCAATTTCACGGTGTCCAATTTCTTGAGTGGTGATGATTGGATTCCTCAAACTGGTGTTCCATACTCTAGTGGATTGATATAG
Protein sequence

>MS.gene20749.t1

MALKHKTIAYLLSILFFVSFHVANGVPPETICSSTVNPTYCKNVFANQNGNIYDYGKISIRKSLSQSRKFMNSIDSQLQGGSSLSQSTIRALEDCRFLAELSFEYLSNTYTTTNQSSNVLPTSQAEDFETFLSAVLTNQQTCLEGLNTIASDQRGKNDLTSSISDDMKLHSVTLALFKKGWVPKSKIRTSWPQNGKHLNFKNGRLPLKMSNKARAIYDSARRNGRKLLQTNTNEDSVVVSDIVVVSQDGSGNFTTINDAVAAAPNNTVASDGYFFIFITKGVYQEYVSIPKNKKYLMMVGEGINQTVITGDHNVVDGFTTFNSATFAVVGQGFVAVNITFRNTAGPSKHQAVAMRSGADMSAFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNGAVVLQNCNIYPRLPLSGQFNSITAQGRTDPNQNTGTSIQNATIKAADDLAPKVGTVQTYLGRPWKEYSRTVFMQSFMDSFINPAGWHEWNGDFALNTLYYAEYGNRGAGSSTVNRVTWPGYHVIGATDAANFTVSNFLSGDDWIPQTGVPYSSGLI