AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene005575

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005575.t1 MTR_1g088020 95.504 556 25 0 1 556 1 556 0.0 1083
MS.gene005575.t1 MTR_1g088225 94.784 556 27 1 1 556 1 554 0.0 1071
MS.gene005575.t1 MTR_1g054880 87.344 561 64 2 1 556 1 559 0.0 996
MS.gene005575.t1 MTR_0458s0010 81.475 556 66 4 1 556 1 519 0.0 882
MS.gene005575.t1 MTR_7g108890 75.376 532 128 2 26 556 34 563 0.0 848
MS.gene005575.t1 MTR_7g108875 66.041 533 176 3 26 556 36 565 0.0 740
MS.gene005575.t1 MTR_7g108900 65.406 529 176 5 26 551 32 556 0.0 716
MS.gene005575.t1 MTR_0458s0030 97.736 265 6 0 292 556 51 315 0.0 545
MS.gene005575.t1 MTR_0458s0030 80.392 51 10 0 1 51 1 51 1.98e-21 95.5
MS.gene005575.t1 MTR_1g088040 97.736 265 6 0 292 556 51 315 0.0 545
MS.gene005575.t1 MTR_1g088040 80.392 51 10 0 1 51 1 51 1.98e-21 95.5
MS.gene005575.t1 MTR_1g083520 50.730 548 236 9 12 555 11 528 0.0 526
MS.gene005575.t1 MTR_3g010770 47.232 542 265 7 32 556 39 576 6.46e-170 494
MS.gene005575.t1 MTR_8g042910 47.310 539 265 7 32 555 78 612 9.31e-169 492
MS.gene005575.t1 MTR_7g108905 50.833 480 205 9 77 551 99 552 8.77e-153 449
MS.gene005575.t1 MTR_4g025690 44.405 563 247 15 8 555 2 513 3.96e-141 418
MS.gene005575.t1 MTR_4g025730 45.981 535 226 16 29 555 30 509 9.52e-139 412
MS.gene005575.t1 MTR_2g044880 45.455 495 215 11 73 555 74 525 1.94e-133 399
MS.gene005575.t1 MTR_4g087980 46.398 472 211 8 88 555 83 516 3.15e-133 398
MS.gene005575.t1 MTR_2g044810 39.789 568 286 9 5 555 6 534 4.42e-133 398
MS.gene005575.t1 MTR_4g124520 46.868 431 195 7 127 555 123 521 1.32e-132 396
MS.gene005575.t1 MTR_8g023310 44.359 523 224 10 51 555 116 589 2.24e-132 398
MS.gene005575.t1 MTR_1g008140 42.251 542 275 10 32 555 86 607 3.49e-131 396
MS.gene005575.t1 MTR_8g104655 42.599 554 282 12 8 555 18 541 4.28e-131 393
MS.gene005575.t1 MTR_4g087970 45.745 470 215 10 91 555 83 517 8.13e-130 389
MS.gene005575.t1 MTR_8g042900 46.008 476 219 12 84 555 140 581 2.11e-129 390
MS.gene005575.t1 MTR_8g104645 55.422 332 144 3 225 555 44 372 7.54e-129 381
MS.gene005575.t1 MTR_3g010780 44.872 468 228 11 88 555 137 574 9.02e-128 386
MS.gene005575.t1 MTR_4g025670 44.444 477 217 8 83 555 57 489 1.17e-127 382
MS.gene005575.t1 MTR_7g112580 44.111 467 229 9 91 555 106 542 1.18e-127 384
MS.gene005575.t1 MTR_7g050980 44.421 475 213 11 91 555 122 555 9.39e-126 380
MS.gene005575.t1 MTR_3g091380 42.301 539 273 11 32 555 82 597 1.97e-125 380
MS.gene005575.t1 MTR_7g050870 43.254 504 237 12 60 555 92 554 3.74e-125 379
MS.gene005575.t1 MTR_7g050950 40.111 541 267 14 31 555 54 553 1.39e-121 369
MS.gene005575.t1 MTR_4g035980 39.777 538 281 11 23 555 40 539 3.76e-120 365
MS.gene005575.t1 MTR_3g033690 39.703 539 262 12 32 555 36 526 8.63e-118 358
MS.gene005575.t1 MTR_3g008720 39.479 537 267 12 32 555 36 527 7.83e-117 356
MS.gene005575.t1 MTR_3g008640 39.153 567 283 14 8 555 4 527 2.64e-116 355
MS.gene005575.t1 MTR_3g008700 39.065 535 272 11 32 555 36 527 1.53e-115 353
MS.gene005575.t1 MTR_7g053360 38.556 568 285 15 8 555 4 527 1.17e-114 350
MS.gene005575.t1 MTR_4g124550 40.851 470 251 7 91 555 146 593 1.59e-114 352
MS.gene005575.t1 MTR_8g023150 40.803 473 244 10 90 555 139 582 3.06e-108 336
MS.gene005575.t1 MTR_1g086210 38.046 481 263 7 91 555 108 569 3.44e-107 333
MS.gene005575.t1 MTR_2g044810 58.333 252 105 0 304 555 214 465 4.87e-107 329
MS.gene005575.t1 MTR_7g112590 34.838 554 319 11 21 554 51 582 1.06e-102 322
MS.gene005575.t1 MTR_1g086190 35.140 535 315 8 31 555 51 563 3.00e-102 320
MS.gene005575.t1 MTR_8g104580 36.957 552 283 13 8 555 10 500 5.09e-101 314
MS.gene005575.t1 MTR_1g083510 38.155 477 268 10 91 554 120 582 9.77e-100 314
MS.gene005575.t1 MTR_0618s0020 59.649 228 91 1 329 555 1 228 4.13e-97 295
MS.gene005575.t1 MTR_1g083470 36.831 467 265 8 91 551 124 566 1.80e-96 305
MS.gene005575.t1 MTR_1g083500 36.831 467 265 8 91 551 124 566 1.80e-96 305
MS.gene005575.t1 MTR_2g044660 49.521 313 151 3 243 555 327 632 2.85e-96 306
MS.gene005575.t1 MTR_7g110560 34.000 550 325 13 17 556 29 550 1.88e-95 301
MS.gene005575.t1 MTR_8g093460 47.785 316 153 5 238 544 228 540 1.07e-94 300
MS.gene005575.t1 MTR_1g083490 33.948 542 322 12 21 551 55 571 1.43e-94 300
MS.gene005575.t1 MTR_4g005170 36.207 464 272 7 91 551 129 571 9.51e-94 300
MS.gene005575.t1 MTR_4g005180 33.579 542 324 12 21 551 37 553 1.12e-92 295
MS.gene005575.t1 MTR_3g095920 37.155 471 247 10 98 548 116 557 2.33e-91 291
MS.gene005575.t1 MTR_7g110490 37.107 477 274 8 88 550 105 569 3.48e-91 291
MS.gene005575.t1 MTR_7g110510 37.107 477 274 8 88 550 105 569 3.48e-91 291
MS.gene005575.t1 MTR_7g110440 35.908 479 281 8 91 555 108 574 1.31e-89 287
MS.gene005575.t1 MTR_4g120280 37.162 444 238 11 125 554 155 571 3.92e-88 283
MS.gene005575.t1 MTR_4g120290 36.343 443 243 10 125 554 155 571 9.31e-86 277
MS.gene005575.t1 MTR_2g436040 35.880 432 240 8 127 552 143 543 1.59e-83 272
MS.gene005575.t1 MTR_5g083620 41.325 317 174 5 244 555 47 356 6.34e-80 255
MS.gene005575.t1 MTR_8g088610 40.181 331 184 6 225 552 54 373 1.22e-78 252
MS.gene005575.t1 MTR_2g436050 32.453 530 314 10 35 554 68 563 7.97e-77 253
MS.gene005575.t1 MTR_7g110420 32.406 503 278 11 91 555 108 586 1.05e-74 248
MS.gene005575.t1 MTR_7g107180 41.587 315 168 6 243 555 7 307 4.39e-72 233
MS.gene005575.t1 MTR_7g110430 33.056 481 258 11 91 555 108 540 7.12e-71 237
MS.gene005575.t1 MTR_2g105560 39.365 315 173 6 243 555 41 339 1.70e-70 230
MS.gene005575.t1 MTR_7g111560 28.938 546 315 14 30 554 62 555 6.08e-68 229
MS.gene005575.t1 MTR_6g012800 35.258 329 179 10 242 553 5 316 6.07e-53 183
MS.gene005575.t1 MTR_1g086540 33.333 357 203 9 199 544 12 344 7.04e-52 181
MS.gene005575.t1 MTR_6g088810 36.066 305 167 7 251 544 90 377 6.39e-51 179
MS.gene005575.t1 MTR_6g024165 35.873 315 170 11 242 544 40 334 9.53e-51 177
MS.gene005575.t1 MTR_7g011530 33.841 328 185 9 242 553 5 316 2.02e-49 173
MS.gene005575.t1 MTR_8g102380 34.395 314 176 7 244 544 81 377 3.68e-49 174
MS.gene005575.t1 MTR_6g042660 32.727 330 183 10 221 544 25 321 1.42e-47 169
MS.gene005575.t1 MTR_7g092780 33.654 312 183 6 243 544 90 387 1.85e-47 170
MS.gene005575.t1 MTR_2g039530 39.032 310 148 11 225 523 40 319 1.83e-46 166
MS.gene005575.t1 MTR_2g033970 32.372 312 182 7 243 545 56 347 7.47e-43 157
MS.gene005575.t1 MTR_4g124740 32.268 313 176 9 242 544 53 339 1.04e-42 156
MS.gene005575.t1 MTR_5g095490 33.442 308 176 7 249 544 67 357 3.13e-42 155
MS.gene005575.t1 MTR_7g102600 30.029 343 210 7 209 544 74 393 5.99e-42 155
MS.gene005575.t1 MTR_1g033050 33.016 315 178 7 242 548 17 306 1.52e-41 152
MS.gene005575.t1 MTR_8g102380 35.878 262 139 7 244 492 81 326 4.52e-41 151
MS.gene005575.t1 MTR_5g012600 31.447 318 189 7 242 547 62 362 6.98e-41 152
MS.gene005575.t1 MTR_5g083590 29.747 316 167 6 243 556 7 269 1.25e-40 148
MS.gene005575.t1 MTR_5g093970 33.548 310 183 7 239 544 39 329 1.10e-39 147
MS.gene005575.t1 MTR_6g042690 32.166 314 177 10 243 544 39 328 3.34e-39 146
MS.gene005575.t1 MTR_5g088300 32.353 306 185 7 239 544 33 316 1.72e-36 139
MS.gene005575.t1 MTR_2g436600 30.671 313 177 10 243 545 55 337 1.86e-36 139
MS.gene005575.t1 MTR_4g051810 30.945 307 191 6 238 544 32 317 2.68e-34 132
MS.gene005575.t1 MTR_5g088290 30.293 307 191 7 239 544 44 328 2.03e-33 130
MS.gene005575.t1 MTR_5g088280 30.293 307 190 7 239 544 35 318 8.14e-31 123
MS.gene005575.t1 MTR_8g036225 30.721 319 177 10 242 544 32 322 1.29e-30 122
MS.gene005575.t1 MTR_4g074290 29.355 310 198 7 240 544 68 361 2.62e-29 119
MS.gene005575.t1 MTR_2g073390 28.846 312 193 10 242 544 70 361 1.75e-27 114
MS.gene005575.t1 MTR_0009s0350 30.634 284 176 7 240 517 68 336 8.14e-27 112
MS.gene005575.t1 MTR_0009s0340 30.634 284 176 7 240 517 68 336 8.14e-27 112
MS.gene005575.t1 MTR_6g025590 36.170 188 101 7 223 400 33 211 9.87e-24 100
MS.gene005575.t1 MTR_0408s0030 35.115 131 71 4 415 544 31 148 1.62e-17 80.1
MS.gene005575.t1 MTR_6g024200 29.651 172 100 4 374 544 125 276 5.57e-15 75.9
MS.gene005575.t1 MTR_4g130790 26.613 248 156 5 246 486 25 253 7.00e-15 75.5
MS.gene005575.t1 MTR_7g102580 43.023 86 39 2 320 400 73 153 1.19e-13 68.9
MS.gene005575.t1 MTR_2g436070 25.839 298 179 12 29 317 57 321 5.30e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene005575.t1 AT1G02810 60.069 576 201 9 8 556 5 578 0.0 681
MS.gene005575.t1 AT4G02330 58.988 573 216 9 1 556 2 572 0.0 672
MS.gene005575.t1 AT2G47550 60.926 540 197 8 22 556 29 559 0.0 654
MS.gene005575.t1 AT3G10720 48.148 540 259 10 32 556 83 616 4.62e-170 496
MS.gene005575.t1 AT5G04970 44.751 543 273 9 32 556 88 621 5.03e-157 462
MS.gene005575.t1 AT2G45220 42.226 566 260 13 1 555 2 511 3.03e-135 403
MS.gene005575.t1 AT2G26440 40.935 535 281 11 30 555 39 547 2.77e-132 397
MS.gene005575.t1 AT3G43270 47.357 473 216 9 91 556 80 526 1.55e-130 391
MS.gene005575.t1 AT3G60730 45.872 436 218 7 126 555 96 519 6.29e-130 389
MS.gene005575.t1 AT5G51490 41.728 544 252 11 32 555 38 536 1.96e-129 389
MS.gene005575.t1 AT4G33220 44.101 517 257 9 53 555 25 523 9.10e-129 387
MS.gene005575.t1 AT1G53830 42.963 540 280 7 29 555 63 587 5.64e-128 387
MS.gene005575.t1 AT1G11580 47.685 432 193 7 127 555 156 557 9.79e-126 380
MS.gene005575.t1 AT1G11580 47.685 432 193 7 127 555 177 578 1.23e-125 380
MS.gene005575.t1 AT3G14310 42.048 547 277 10 32 555 63 592 5.85e-125 379
MS.gene005575.t1 AT1G23200 40.138 578 274 15 7 555 20 554 8.42e-125 377
MS.gene005575.t1 AT3G05620 43.035 481 215 8 91 555 104 541 2.02e-124 376
MS.gene005575.t1 AT4G33220 54.867 339 143 3 218 555 73 402 3.29e-124 371
MS.gene005575.t1 AT3G10720 63.077 260 96 0 297 556 1 260 1.52e-123 363
MS.gene005575.t1 AT5G53370 46.544 434 206 9 127 555 173 585 7.53e-123 374
MS.gene005575.t1 AT5G53370 46.544 434 206 9 127 555 209 621 2.65e-122 374
MS.gene005575.t1 AT3G49220 46.119 438 210 9 123 555 180 596 5.74e-122 372
MS.gene005575.t1 AT3G49220 46.119 438 210 9 123 555 225 641 1.75e-121 372
MS.gene005575.t1 AT5G04960 40.239 502 255 11 57 553 101 562 7.38e-121 368
MS.gene005575.t1 AT4G02300 40.642 561 286 12 4 555 10 532 7.54e-121 367
MS.gene005575.t1 AT5G51500 39.850 532 279 10 32 555 42 540 9.53e-119 362
MS.gene005575.t1 AT3G10710 39.326 534 280 13 26 555 68 561 1.40e-117 359
MS.gene005575.t1 AT4G02320 41.754 479 248 9 81 555 67 518 1.36e-116 355
MS.gene005575.t1 AT2G43050 42.138 477 215 9 88 556 94 517 1.96e-115 352
MS.gene005575.t1 AT3G05610 39.496 476 253 7 88 556 119 566 2.93e-115 357
MS.gene005575.t1 AT1G53840 38.208 547 285 13 27 549 64 581 1.42e-114 352
MS.gene005575.t1 AT2G26450 37.732 538 288 12 29 555 110 611 1.47e-114 353
MS.gene005575.t1 AT4G00190 39.736 531 251 16 31 555 7 474 2.98e-113 345
MS.gene005575.t1 AT3G59010 44.773 440 194 10 126 556 130 529 9.27e-113 346
MS.gene005575.t1 AT4G33230 36.852 540 299 13 29 555 96 606 4.96e-108 336
MS.gene005575.t1 AT5G27870 38.298 470 257 6 91 555 121 562 7.57e-106 334
MS.gene005575.t1 AT5G49180 42.155 427 230 6 126 551 156 566 3.68e-104 325
MS.gene005575.t1 AT3G47400 40.812 468 247 6 91 555 154 594 4.60e-104 325
MS.gene005575.t1 AT3G14300 41.401 471 236 11 91 550 525 966 2.54e-102 330
MS.gene005575.t1 AT4G03930 42.227 431 216 9 126 555 126 524 7.06e-101 315
MS.gene005575.t1 AT5G20860 35.507 552 276 14 32 555 39 538 2.36e-100 314
MS.gene005575.t1 AT3G27980 41.259 429 211 8 130 555 107 497 2.14e-99 310
MS.gene005575.t1 AT3G06830 40.185 433 238 8 126 553 150 566 2.69e-99 312
MS.gene005575.t1 AT1G11590 41.531 431 219 10 126 555 126 524 8.21e-98 307
MS.gene005575.t1 AT5G09760 47.097 310 151 6 243 543 267 572 5.46e-86 278
MS.gene005575.t1 AT5G09760 47.097 310 151 6 243 543 241 546 5.75e-86 277
MS.gene005575.t1 AT4G15980 44.277 332 179 3 223 553 374 700 3.58e-85 279
MS.gene005575.t1 AT4G15980 44.277 332 179 3 223 553 384 710 6.05e-85 278
MS.gene005575.t1 AT1G11370 52.869 244 112 1 243 486 46 286 1.09e-84 265
MS.gene005575.t1 AT5G64640 36.786 473 247 11 98 548 160 602 2.99e-84 274
MS.gene005575.t1 AT5G20860 32.653 539 255 13 32 542 39 497 8.93e-79 257
MS.gene005575.t1 AT2G47030 43.175 315 172 4 244 555 278 588 3.14e-74 247
MS.gene005575.t1 AT2G47040 31.306 559 343 12 31 555 44 595 2.65e-72 242
MS.gene005575.t1 AT3G62170 30.713 547 352 12 29 555 49 588 7.18e-70 235
MS.gene005575.t1 AT5G19730 35.350 314 175 7 242 544 85 381 1.60e-51 181
MS.gene005575.t1 AT3G29090 35.714 294 163 6 237 519 1 279 1.23e-49 174
MS.gene005575.t1 AT3G24130 35.093 322 174 8 243 550 34 334 3.36e-48 171
MS.gene005575.t1 AT2G21610 33.003 303 183 6 242 544 50 332 3.81e-48 171
MS.gene005575.t1 AT2G21610 33.003 303 183 6 242 544 55 337 5.63e-48 171
MS.gene005575.t1 AT1G05310 35.256 312 178 6 243 544 91 388 6.96e-48 171
MS.gene005575.t1 AT2G36710 32.698 315 191 5 242 549 90 390 3.31e-47 170
MS.gene005575.t1 AT5G18990 37.457 291 147 8 243 518 29 299 1.43e-46 166
MS.gene005575.t1 AT2G36700 31.494 308 191 5 242 544 41 333 1.98e-45 163
MS.gene005575.t1 AT3G17060 31.090 312 186 7 243 545 46 337 6.65e-45 162
MS.gene005575.t1 AT2G19150 29.721 323 197 8 239 551 37 339 1.02e-42 156
MS.gene005575.t1 AT5G47500 31.111 315 188 7 242 544 60 357 3.67e-41 152
MS.gene005575.t1 AT5G07430 31.746 315 194 7 234 544 58 355 6.91e-41 152
MS.gene005575.t1 AT5G55590 32.390 318 186 7 239 544 78 378 4.94e-40 150
MS.gene005575.t1 AT5G26810 32.132 333 194 10 225 544 20 333 2.37e-39 147
MS.gene005575.t1 AT2G47280 31.579 323 185 10 243 550 35 336 5.06e-38 143
MS.gene005575.t1 AT2G19150 32.443 262 149 7 239 490 37 280 1.31e-37 140
MS.gene005575.t1 AT5G55590 33.712 264 148 6 239 490 78 326 2.26e-37 141
MS.gene005575.t1 AT5G07420 30.293 307 193 7 242 544 66 355 4.02e-36 138
MS.gene005575.t1 AT1G44980 42.515 167 93 1 243 409 78 241 8.32e-35 132
MS.gene005575.t1 AT5G61680 31.068 309 189 9 242 544 68 358 1.40e-33 131
MS.gene005575.t1 AT1G69940 28.571 308 197 8 242 544 66 355 2.73e-32 128
MS.gene005575.t1 AT5G07410 28.571 308 197 8 242 544 66 355 5.10e-32 127

Find 110 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACAGTTGACTTCATATT+TGG 0.172980 1.1:+65067102 MS.gene005575:CDS
TCACTAGCAACCGTGTTATT+TGG 0.218847 1.1:-65066528 None:intergenic
GTATAGCTGCAAATGGTTTC+TGG 0.244833 1.1:-65065820 None:intergenic
ATCATCACCACTCAAGAAAT+TGG 0.256704 1.1:-65067535 None:intergenic
GAAATTGGATACCGTGAAAT+TGG 0.260830 1.1:-65067520 None:intergenic
TTGTAGCTTTGAAGGGTATC+AGG 0.324620 1.1:+65067026 MS.gene005575:CDS
CTTTGTCTCAATCCACAATT+CGG 0.336867 1.1:+65065986 MS.gene005575:CDS
TATTAAAGCAGCTGATGATT+TGG 0.337337 1.1:+65067257 MS.gene005575:CDS
AATGGAAACATTTATGACTA+TGG 0.349973 1.1:+65065883 MS.gene005575:CDS
AACGTATTTGCCAATCAAAA+TGG 0.357020 1.1:+65065865 MS.gene005575:CDS
AGAACATCATGGCCACAAAA+TGG 0.357335 1.1:+65066300 MS.gene005575:CDS
TGGTCTGGTTATCATGTTAT+TGG 0.358685 1.1:+65067483 MS.gene005575:CDS
GACTAACCAACAAACATGTT+TGG 0.374749 1.1:+65066146 MS.gene005575:CDS
TTCTATAGTTGTAGCTTTGA+AGG 0.378500 1.1:+65067018 MS.gene005575:CDS
TATATCAATCCACTAGAGTA+TGG 0.378755 1.1:-65067579 None:intergenic
TTTACAATAAGTAGGGTTTA+CGG 0.380121 1.1:-65065842 None:intergenic
TATTGAATTCATGAACTTAC+GGG 0.391914 1.1:-65065938 None:intergenic
GTAGCAGTTAACATAACATT+TGG 0.392798 1.1:+65066931 MS.gene005575:CDS
ACATTCAATTCGGCTACATT+TGG 0.394202 1.1:+65066696 MS.gene005575:CDS
ACCCTGCTGGTTGGCATGAA+TGG 0.394563 1.1:+65067376 MS.gene005575:CDS
GCAACTGCTTGGTGTTTGCT+TGG 0.397641 1.1:-65066967 None:intergenic
CACTTCTCATTGCAACTGCT+TGG 0.401773 1.1:-65066978 None:intergenic
GACCTTCCAAACATGTTTGT+TGG 0.404138 1.1:-65066152 None:intergenic
TATCGAGAATGCGATATATA+CGG 0.406490 1.1:+65067081 MS.gene005575:CDS
TTTGTCTCAATCCACAATTC+GGG 0.413019 1.1:+65065987 MS.gene005575:CDS
GCTTTATTTGACATCTTCAA+TGG 0.419717 1.1:-65066354 None:intergenic
AGATTGATTTGTTGTTGTAT+AGG 0.420233 1.1:-65066064 None:intergenic
TTCAAAGCTACAACTATAGA+AGG 0.420977 1.1:-65067016 None:intergenic
CTATTGAATTCATGAACTTA+CGG 0.421755 1.1:-65065939 None:intergenic
AAGTCTTCTGCTTGAGAAGT+TGG 0.427453 1.1:-65066099 None:intergenic
ATTTCTTGAGTGGTGATGAT+TGG 0.428264 1.1:+65067538 MS.gene005575:CDS
GAACAAGATCGAAAGTAGAT+AGG 0.433031 1.1:-65065764 None:intergenic
TTCCATTCATGCCAACCAGC+AGG 0.438837 1.1:-65067378 None:intergenic
TGGTTTCACAACATTCAATT+CGG 0.439204 1.1:+65066686 MS.gene005575:CDS
GTTTGTACCGTTCCAACATT+CGG 0.443951 1.1:-65067282 None:intergenic
CATTGTATACACATTCTCTA+AGG 0.446591 1.1:+65067052 MS.gene005575:CDS
ATTGTCGTTGTTAGTCAAGA+TGG 0.461728 1.1:+65066462 MS.gene005575:CDS
GTATCATTCCATGTAGCAAA+TGG 0.462739 1.1:+65065790 MS.gene005575:CDS
GGAGACCACAATGTTGTTGA+TGG 0.463514 1.1:+65066666 MS.gene005575:CDS
ATACTCTTGATACACACCTT+TGG 0.465883 1.1:-65066574 None:intergenic
ACCCTAGCCTTGTTCAAGAA+AGG 0.466853 1.1:+65066255 MS.gene005575:CDS
GAACTTACGGGATTGCGATA+AGG 0.466990 1.1:-65065926 None:intergenic
AGCCTTGTTCAAGAAAGGTT+GGG 0.473203 1.1:+65066260 MS.gene005575:CDS
TAGCCTTGTTCAAGAAAGGT+TGG 0.480777 1.1:+65066259 MS.gene005575:CDS
TACCGATGCAGCCAATTTCA+CGG 0.485475 1.1:+65067509 MS.gene005575:CDS
TACGAACCCTTGACCAACTA+CGG 0.486181 1.1:-65066911 None:intergenic
GTGGAACACCATTTGCTACA+TGG 0.489190 1.1:-65065798 None:intergenic
GTTTACGGTATAGCTGCAAA+TGG 0.494007 1.1:-65065827 None:intergenic
ATCAATCAAACAGTTATCAC+TGG 0.497805 1.1:+65066645 MS.gene005575:CDS
AACCTTTCTTGAACAAGGCT+AGG 0.499650 1.1:-65066257 None:intergenic
ATGATTCTGCTAGACGCAAT+GGG 0.504675 1.1:+65066388 MS.gene005575:CDS
TGTTTGTTGGTTAGTCAAAA+CGG 0.505795 1.1:-65066139 None:intergenic
TATCCTCGCCTTCCGCTAAG+TGG 0.509384 1.1:+65067159 MS.gene005575:CDS
AGCTACCGCGTCATTGATAG+TGG 0.510094 1.1:-65066499 None:intergenic
ATAACATTTGGCAACACCGC+TGG 0.512526 1.1:+65066943 MS.gene005575:CDS
TTCTTCATATTTATCACCAA+AGG 0.513617 1.1:+65066558 MS.gene005575:CDS
TATGATTCTGCTAGACGCAA+TGG 0.514899 1.1:+65066387 MS.gene005575:CDS
GACCGTGGAAAGAATATTCA+AGG 0.515066 1.1:+65067316 MS.gene005575:CDS
TCAATAGACTCACAATTTCA+AGG 0.516078 1.1:+65065955 MS.gene005575:CDS
AGTTCGACCTTGAGCTGTGA+TGG 0.517049 1.1:-65067193 None:intergenic
GTTGACTTCATATTTGGAAA+CGG 0.519141 1.1:+65067108 MS.gene005575:CDS
GTCCTTGAATATTCTTTCCA+CGG 0.519966 1.1:-65067318 None:intergenic
GACAGTTTCATAAACCCTGC+TGG 0.520411 1.1:+65067363 MS.gene005575:CDS
TCTATAGTTGTAGCTTTGAA+GGG 0.521345 1.1:+65067019 MS.gene005575:CDS
ATTCTTTCCACGGTCTCCCT+AGG 0.521753 1.1:-65067308 None:intergenic
TACCGTGAAATTGGCTGCAT+CGG 0.524322 1.1:-65067511 None:intergenic
GATGATTTGGCTCCGAATGT+TGG 0.529941 1.1:+65067270 MS.gene005575:CDS
GTGAATCGAGTGACATGGTC+TGG 0.530018 1.1:+65067468 MS.gene005575:CDS
ACTTTGTACTATGCAGAATA+CGG 0.531445 1.1:+65067420 MS.gene005575:CDS
AATAACACGGTTGCTAGTGA+TGG 0.541511 1.1:+65066531 MS.gene005575:CDS
GAATACGGCAACAGAGGAGC+GGG 0.544190 1.1:+65067435 MS.gene005575:CDS
CAAGCAGTTGCAATGAGAAG+TGG 0.548536 1.1:+65066979 MS.gene005575:CDS
TACTTGATGATGGTTGGAGA+GGG 0.549006 1.1:+65066621 MS.gene005575:CDS
ACGGTATCCAATTTCTTGAG+TGG 0.550073 1.1:+65067528 MS.gene005575:CDS
AACCAACAAACATGTTTGGA+AGG 0.550080 1.1:+65066150 MS.gene005575:CDS
GAGTATGGAACACCAGTTTG+AGG 0.551293 1.1:-65067564 None:intergenic
GATGATTGGATTCCTCAAAC+TGG 0.552199 1.1:+65067552 MS.gene005575:CDS
ACTGGTGTTCCATACTCTAG+TGG 0.555255 1.1:+65067570 MS.gene005575:CDS
ACCTTTCTTGAACAAGGCTA+GGG 0.557941 1.1:-65066256 None:intergenic
GGAACGGTACAAACATACCT+AGG 0.559615 1.1:+65067291 MS.gene005575:CDS
GAATTGAATTGTCCACTTAG+CGG 0.559760 1.1:-65067171 None:intergenic
TTCAATTCCATCACAGCTCA+AGG 0.560247 1.1:+65067186 MS.gene005575:CDS
GTTGTTAGTCAAGATGGAAG+TGG 0.562167 1.1:+65066468 MS.gene005575:CDS
ATCTTCAAGAGCCCGAATTG+TGG 0.568143 1.1:-65065998 None:intergenic
AAAGTAAAATAAGAACATCA+TGG 0.577883 1.1:+65066289 MS.gene005575:CDS
GTTTCATAAACCCTGCTGGT+TGG 0.584669 1.1:+65067367 MS.gene005575:CDS
TCCATTCATGCCAACCAGCA+GGG 0.585336 1.1:-65067377 None:intergenic
TTTCAGCCGTAGTTGGTCAA+GGG 0.587591 1.1:+65066905 MS.gene005575:intron
TTGGTGTTTGCTTGGTCCAG+CGG 0.591249 1.1:-65066959 None:intergenic
AACCCAACCTTTCTTGAACA+AGG 0.595250 1.1:-65066262 None:intergenic
GTACTTGATGATGGTTGGAG+AGG 0.601393 1.1:+65066620 MS.gene005575:CDS
ACTGACCCAAATCAAAACAC+TGG 0.605088 1.1:+65067213 MS.gene005575:CDS
TGAATTGTCCACTTAGCGGA+AGG 0.605583 1.1:-65067167 None:intergenic
TAGCTGCAAATGGTTTCTGG+TGG 0.617891 1.1:-65065817 None:intergenic
TGTCCACTTAGCGGAAGGCG+AGG 0.623788 1.1:-65067162 None:intergenic
AAAGCTACAACTATAGAAGG+TGG 0.633511 1.1:-65067013 None:intergenic
ATTCAAAACTCAATTCAGCA+AGG 0.638769 1.1:-65066029 None:intergenic
GCTGGTTGGCATGAATGGAA+TGG 0.651490 1.1:+65067381 MS.gene005575:CDS
GTTCTTCACTCTCTGATCAG+AGG 0.651544 1.1:-65066187 None:intergenic
GCATCGTGAATCGAGTGACA+TGG 0.653933 1.1:+65067463 MS.gene005575:CDS
TTTGGCTCCGAATGTTGGAA+CGG 0.654436 1.1:+65067275 MS.gene005575:CDS
TATGCAGAATACGGCAACAG+AGG 0.658760 1.1:+65067429 MS.gene005575:CDS
AGAATACGGCAACAGAGGAG+CGG 0.660300 1.1:+65067434 MS.gene005575:CDS
AAACATACCTAGGGAGACCG+TGG 0.663984 1.1:+65067301 MS.gene005575:CDS
GAACGGTACAAACATACCTA+GGG 0.671516 1.1:+65067292 MS.gene005575:CDS
AGCTGCTGCACCAAATAACA+CGG 0.671662 1.1:+65066518 MS.gene005575:CDS
CTTTACCACTATCAATGACG+CGG 0.681296 1.1:+65066494 MS.gene005575:CDS
TGAAACCATCAACAACATTG+TGG 0.688631 1.1:-65066671 None:intergenic
TACTAATGAAGATAGTGTTG+TGG 0.688812 1.1:+65066431 MS.gene005575:CDS
ATAGACTCACAATTTCAAGG+AGG 0.738552 1.1:+65065958 MS.gene005575:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTGTTTAGCTATATA+CGG + chr1.1:65066785-65066804 MS.gene005575:intron 15.0%
!! AAAGTAAAATAAGAACATCA+TGG + chr1.1:65066289-65066308 MS.gene005575:CDS 20.0%
!! AAGCATTTGAATTTCAAAAA+TGG + chr1.1:65066324-65066343 MS.gene005575:CDS 20.0%
!!! AATACGTTTTTACAATAAGT+AGG - chr1.1:65065853-65065872 None:intergenic 20.0%
!!! ATAAATGTTTCCATTTTGAT+TGG - chr1.1:65065878-65065897 None:intergenic 20.0%
!!! ATACGTTTTTACAATAAGTA+GGG - chr1.1:65065852-65065871 None:intergenic 20.0%
!!! ATCAAGTACTTTTTGTTTTT+GGG - chr1.1:65066609-65066628 None:intergenic 20.0%
!!! GATGTTCTTATTTTACTTTT+AGG - chr1.1:65066288-65066307 None:intergenic 20.0%
! AATGGAAACATTTATGACTA+TGG + chr1.1:65065883-65065902 MS.gene005575:CDS 25.0%
! ATAGATTATCTTCAACATTG+TGG - chr1.1:65066847-65066866 None:intergenic 25.0%
! CTATTGAATTCATGAACTTA+CGG - chr1.1:65065942-65065961 None:intergenic 25.0%
! TATTGAATTCATGAACTTAC+GGG - chr1.1:65065941-65065960 None:intergenic 25.0%
! TGGTAAGCAAATTTAATACT+TGG + chr1.1:65066716-65066735 MS.gene005575:intron 25.0%
! TTCTTCATATTTATCACCAA+AGG + chr1.1:65066558-65066577 MS.gene005575:CDS 25.0%
! TTTACAATAAGTAGGGTTTA+CGG - chr1.1:65065845-65065864 None:intergenic 25.0%
!! AGTTTTTATGCAATCTTTCA+TGG + chr1.1:65067341-65067360 MS.gene005575:CDS 25.0%
!!! AAACAAAAAGTACTTGATGA+TGG + chr1.1:65066611-65066630 MS.gene005575:CDS 25.0%
!!! AGATTGATTTGTTGTTGTAT+AGG - chr1.1:65066067-65066086 None:intergenic 25.0%
!!! CATCAAGTACTTTTTGTTTT+TGG - chr1.1:65066610-65066629 None:intergenic 25.0%
AACGTATTTGCCAATCAAAA+TGG + chr1.1:65065865-65065884 MS.gene005575:CDS 30.0%
ACTTTGTACTATGCAGAATA+CGG + chr1.1:65067420-65067439 MS.gene005575:CDS 30.0%
ATCAATCAAACAGTTATCAC+TGG + chr1.1:65066645-65066664 MS.gene005575:CDS 30.0%
ATTCAAAACTCAATTCAGCA+AGG - chr1.1:65066032-65066051 None:intergenic 30.0%
CATTGTATACACATTCTCTA+AGG + chr1.1:65067052-65067071 MS.gene005575:CDS 30.0%
GCAAATTTAATACTTGGAGT+TGG + chr1.1:65066722-65066741 MS.gene005575:intron 30.0%
GTAGCAGTTAACATAACATT+TGG + chr1.1:65066931-65066950 MS.gene005575:CDS 30.0%
TACTAATGAAGATAGTGTTG+TGG + chr1.1:65066431-65066450 MS.gene005575:CDS 30.0%
TATATCAATCCACTAGAGTA+TGG - chr1.1:65067582-65067601 None:intergenic 30.0%
TATCGAGAATGCGATATATA+CGG + chr1.1:65067081-65067100 MS.gene005575:CDS 30.0%
TATTAAAGCAGCTGATGATT+TGG + chr1.1:65067257-65067276 MS.gene005575:CDS 30.0%
TCAATAGACTCACAATTTCA+AGG + chr1.1:65065955-65065974 MS.gene005575:CDS 30.0%
TGGTTTCACAACATTCAATT+CGG + chr1.1:65066686-65066705 MS.gene005575:CDS 30.0%
TTCAAAGCTACAACTATAGA+AGG - chr1.1:65067019-65067038 None:intergenic 30.0%
! GTTGACTTCATATTTGGAAA+CGG + chr1.1:65067108-65067127 MS.gene005575:CDS 30.0%
! TGTTTGTTGGTTAGTCAAAA+CGG - chr1.1:65066142-65066161 None:intergenic 30.0%
!! AAAAAGTACTTGATGATGGT+TGG + chr1.1:65066615-65066634 MS.gene005575:CDS 30.0%
!! GCTTTATTTGACATCTTCAA+TGG - chr1.1:65066357-65066376 None:intergenic 30.0%
!! TCTATAGTTGTAGCTTTGAA+GGG + chr1.1:65067019-65067038 MS.gene005575:CDS 30.0%
!! TTCTATAGTTGTAGCTTTGA+AGG + chr1.1:65067018-65067037 MS.gene005575:CDS 30.0%
!!! AAGTTCCAGTGTTTTGATTT+GGG - chr1.1:65067221-65067240 None:intergenic 30.0%
AAAGCTACAACTATAGAAGG+TGG - chr1.1:65067016-65067035 None:intergenic 35.0%
AACCAACAAACATGTTTGGA+AGG + chr1.1:65066150-65066169 MS.gene005575:CDS 35.0%
ACATTCAATTCGGCTACATT+TGG + chr1.1:65066696-65066715 MS.gene005575:CDS 35.0%
ATACTCTTGATACACACCTT+TGG - chr1.1:65066577-65066596 None:intergenic 35.0%
ATAGACTCACAATTTCAAGG+AGG + chr1.1:65065958-65065977 MS.gene005575:CDS 35.0%
ATCATCACCACTCAAGAAAT+TGG - chr1.1:65067538-65067557 None:intergenic 35.0%
ATTGTCGTTGTTAGTCAAGA+TGG + chr1.1:65066462-65066481 MS.gene005575:CDS 35.0%
CTTTGTCTCAATCCACAATT+CGG + chr1.1:65065986-65066005 MS.gene005575:CDS 35.0%
GAAATTGGATACCGTGAAAT+TGG - chr1.1:65067523-65067542 None:intergenic 35.0%
GAACAAGATCGAAAGTAGAT+AGG - chr1.1:65065767-65065786 None:intergenic 35.0%
GAATTGAATTGTCCACTTAG+CGG - chr1.1:65067174-65067193 None:intergenic 35.0%
GACTAACCAACAAACATGTT+TGG + chr1.1:65066146-65066165 MS.gene005575:CDS 35.0%
GTATCATTCCATGTAGCAAA+TGG + chr1.1:65065790-65065809 MS.gene005575:CDS 35.0%
GTCCTTGAATATTCTTTCCA+CGG - chr1.1:65067321-65067340 None:intergenic 35.0%
GTGGTCCAAAAATCTTCAAT+TGG - chr1.1:65066828-65066847 None:intergenic 35.0%
TGAAACCATCAACAACATTG+TGG - chr1.1:65066674-65066693 None:intergenic 35.0%
TGGTCTGGTTATCATGTTAT+TGG + chr1.1:65067483-65067502 MS.gene005575:CDS 35.0%
TTTGTCTCAATCCACAATTC+GGG + chr1.1:65065987-65066006 MS.gene005575:CDS 35.0%
! AGCAGCCAATTGAAGATTTT+TGG + chr1.1:65066820-65066839 MS.gene005575:intron 35.0%
! ATTTCTTGAGTGGTGATGAT+TGG + chr1.1:65067538-65067557 MS.gene005575:CDS 35.0%
! GGAACAGTTGACTTCATATT+TGG + chr1.1:65067102-65067121 MS.gene005575:CDS 35.0%
!!! GAAGTTCCAGTGTTTTGATT+TGG - chr1.1:65067222-65067241 None:intergenic 35.0%
!!! TTCAAATGCTTGCCATTTTG+TGG - chr1.1:65066315-65066334 None:intergenic 35.0%
AACCCAACCTTTCTTGAACA+AGG - chr1.1:65066265-65066284 None:intergenic 40.0%
AACCTTTCTTGAACAAGGCT+AGG - chr1.1:65066260-65066279 None:intergenic 40.0%
AAGTCTTCTGCTTGAGAAGT+TGG - chr1.1:65066102-65066121 None:intergenic 40.0%
AATAACACGGTTGCTAGTGA+TGG + chr1.1:65066531-65066550 MS.gene005575:CDS 40.0%
ACCTTTCTTGAACAAGGCTA+GGG - chr1.1:65066259-65066278 None:intergenic 40.0%
ACGGTATCCAATTTCTTGAG+TGG + chr1.1:65067528-65067547 MS.gene005575:CDS 40.0%
ACTGACCCAAATCAAAACAC+TGG + chr1.1:65067213-65067232 MS.gene005575:CDS 40.0%
AGAACATCATGGCCACAAAA+TGG + chr1.1:65066300-65066319 MS.gene005575:CDS 40.0%
ATGATTCTGCTAGACGCAAT+GGG + chr1.1:65066388-65066407 MS.gene005575:CDS 40.0%
CTTTACCACTATCAATGACG+CGG + chr1.1:65066494-65066513 MS.gene005575:CDS 40.0%
GAACGGTACAAACATACCTA+GGG + chr1.1:65067292-65067311 MS.gene005575:CDS 40.0%
GACCGTGGAAAGAATATTCA+AGG + chr1.1:65067316-65067335 MS.gene005575:CDS 40.0%
GACCTTCCAAACATGTTTGT+TGG - chr1.1:65066155-65066174 None:intergenic 40.0%
GATGATTGGATTCCTCAAAC+TGG + chr1.1:65067552-65067571 MS.gene005575:CDS 40.0%
GTATAGCTGCAAATGGTTTC+TGG - chr1.1:65065823-65065842 None:intergenic 40.0%
GTTGTTAGTCAAGATGGAAG+TGG + chr1.1:65066468-65066487 MS.gene005575:CDS 40.0%
GTTTACGGTATAGCTGCAAA+TGG - chr1.1:65065830-65065849 None:intergenic 40.0%
GTTTGTACCGTTCCAACATT+CGG - chr1.1:65067285-65067304 None:intergenic 40.0%
TATGATTCTGCTAGACGCAA+TGG + chr1.1:65066387-65066406 MS.gene005575:CDS 40.0%
TTCAATTCCATCACAGCTCA+AGG + chr1.1:65067186-65067205 MS.gene005575:CDS 40.0%
! TCACTAGCAACCGTGTTATT+TGG - chr1.1:65066531-65066550 None:intergenic 40.0%
! TGTGAATTTTCAGCCGTAGT+TGG + chr1.1:65066898-65066917 MS.gene005575:intron 40.0%
!! AGCCTTGTTCAAGAAAGGTT+GGG + chr1.1:65066260-65066279 MS.gene005575:CDS 40.0%
!! TACTTGATGATGGTTGGAGA+GGG + chr1.1:65066621-65066640 MS.gene005575:CDS 40.0%
!! TAGCCTTGTTCAAGAAAGGT+TGG + chr1.1:65066259-65066278 MS.gene005575:CDS 40.0%
!! TTGTAGCTTTGAAGGGTATC+AGG + chr1.1:65067026-65067045 MS.gene005575:CDS 40.0%
ACTGGTGTTCCATACTCTAG+TGG + chr1.1:65067570-65067589 MS.gene005575:CDS 45.0%
AGCTGCTGCACCAAATAACA+CGG + chr1.1:65066518-65066537 MS.gene005575:CDS 45.0%
ATAACATTTGGCAACACCGC+TGG + chr1.1:65066943-65066962 MS.gene005575:CDS 45.0%
ATCTTCAAGAGCCCGAATTG+TGG - chr1.1:65066001-65066020 None:intergenic 45.0%
CAAGCAGTTGCAATGAGAAG+TGG + chr1.1:65066979-65066998 MS.gene005575:CDS 45.0%
CACTTCTCATTGCAACTGCT+TGG - chr1.1:65066981-65067000 None:intergenic 45.0%
GAACTTACGGGATTGCGATA+AGG - chr1.1:65065929-65065948 None:intergenic 45.0%
GACAGTTTCATAAACCCTGC+TGG + chr1.1:65067363-65067382 MS.gene005575:CDS 45.0%
GAGTATGGAACACCAGTTTG+AGG - chr1.1:65067567-65067586 None:intergenic 45.0%
GGAACGGTACAAACATACCT+AGG + chr1.1:65067291-65067310 MS.gene005575:CDS 45.0%
GGAGACCACAATGTTGTTGA+TGG + chr1.1:65066666-65066685 MS.gene005575:CDS 45.0%
GTTCTTCACTCTCTGATCAG+AGG - chr1.1:65066190-65066209 None:intergenic 45.0%
GTTTCATAAACCCTGCTGGT+TGG + chr1.1:65067367-65067386 MS.gene005575:CDS 45.0%
TACCGATGCAGCCAATTTCA+CGG + chr1.1:65067509-65067528 MS.gene005575:CDS 45.0%
TACCGTGAAATTGGCTGCAT+CGG - chr1.1:65067514-65067533 None:intergenic 45.0%
TACGAACCCTTGACCAACTA+CGG - chr1.1:65066914-65066933 None:intergenic 45.0%
TATGCAGAATACGGCAACAG+AGG + chr1.1:65067429-65067448 MS.gene005575:CDS 45.0%
TGAATTGTCCACTTAGCGGA+AGG - chr1.1:65067170-65067189 None:intergenic 45.0%
TTTCAGCCGTAGTTGGTCAA+GGG + chr1.1:65066905-65066924 MS.gene005575:intron 45.0%
! GATGATTTGGCTCCGAATGT+TGG + chr1.1:65067270-65067289 MS.gene005575:CDS 45.0%
! GTGGAACACCATTTGCTACA+TGG - chr1.1:65065801-65065820 None:intergenic 45.0%
! TAGCTGCAAATGGTTTCTGG+TGG - chr1.1:65065820-65065839 None:intergenic 45.0%
! TTTGGCTCCGAATGTTGGAA+CGG + chr1.1:65067275-65067294 MS.gene005575:CDS 45.0%
! TTTTCAGCCGTAGTTGGTCA+AGG + chr1.1:65066904-65066923 MS.gene005575:intron 45.0%
!! ACCCTAGCCTTGTTCAAGAA+AGG + chr1.1:65066255-65066274 MS.gene005575:CDS 45.0%
!! GTACTTGATGATGGTTGGAG+AGG + chr1.1:65066620-65066639 MS.gene005575:CDS 45.0%
AAACATACCTAGGGAGACCG+TGG + chr1.1:65067301-65067320 MS.gene005575:CDS 50.0%
AGAATACGGCAACAGAGGAG+CGG + chr1.1:65067434-65067453 MS.gene005575:CDS 50.0%
AGCTACCGCGTCATTGATAG+TGG - chr1.1:65066502-65066521 None:intergenic 50.0%
AGTTCGACCTTGAGCTGTGA+TGG - chr1.1:65067196-65067215 None:intergenic 50.0%
ATTCTTTCCACGGTCTCCCT+AGG - chr1.1:65067311-65067330 None:intergenic 50.0%
GCATCGTGAATCGAGTGACA+TGG + chr1.1:65067463-65067482 MS.gene005575:CDS 50.0%
GCTGGTTGGCATGAATGGAA+TGG + chr1.1:65067381-65067400 MS.gene005575:CDS 50.0%
GTGAATCGAGTGACATGGTC+TGG + chr1.1:65067468-65067487 MS.gene005575:CDS 50.0%
TCCATTCATGCCAACCAGCA+GGG - chr1.1:65067380-65067399 None:intergenic 50.0%
TTCCATTCATGCCAACCAGC+AGG - chr1.1:65067381-65067400 None:intergenic 50.0%
! TTGGTGTTTGCTTGGTCCAG+CGG - chr1.1:65066962-65066981 None:intergenic 50.0%
!! GCAACTGCTTGGTGTTTGCT+TGG - chr1.1:65066970-65066989 None:intergenic 50.0%
GAATACGGCAACAGAGGAGC+GGG + chr1.1:65067435-65067454 MS.gene005575:CDS 55.0%
TATCCTCGCCTTCCGCTAAG+TGG + chr1.1:65067159-65067178 MS.gene005575:CDS 55.0%
! ACCCTGCTGGTTGGCATGAA+TGG + chr1.1:65067376-65067395 MS.gene005575:CDS 55.0%
TGTCCACTTAGCGGAAGGCG+AGG - chr1.1:65067165-65067184 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 65065739 65067602 65065739 ID=MS.gene005575
chr1.1 mRNA 65065739 65067602 65065739 ID=MS.gene005575.t1;Parent=MS.gene005575
chr1.1 exon 65065739 65066717 65065739 ID=MS.gene005575.t1.exon1;Parent=MS.gene005575.t1
chr1.1 CDS 65065739 65066717 65065739 ID=cds.MS.gene005575.t1;Parent=MS.gene005575.t1
chr1.1 exon 65066911 65067602 65066911 ID=MS.gene005575.t1.exon2;Parent=MS.gene005575.t1
chr1.1 CDS 65066911 65067602 65066911 ID=cds.MS.gene005575.t1;Parent=MS.gene005575.t1
Gene Sequence

>MS.gene005575

ATGGCTCTCAAACACAAAACTATTGCCTATCTACTTTCGATCTTGTTCTTTGTATCATTCCATGTAGCAAATGGTGTTCCACCAGAAACCATTTGCAGCTATACCGTAAACCCTACTTATTGTAAAAACGTATTTGCCAATCAAAATGGAAACATTTATGACTATGGTAAAATTTCAATTCGAAAATCCTTATCGCAATCCCGTAAGTTCATGAATTCAATAGACTCACAATTTCAAGGAGGTTCATCTTTGTCTCAATCCACAATTCGGGCTCTTGAAGATTGTCGATTCCTTGCTGAATTGAGTTTTGAATACTTATCAAACACCTATACAACAACAAATCAATCTAGTAATGTTCTTCCAACTTCTCAAGCAGAAGACTTTGAAACTTTTCTTAGTGCCGTTTTGACTAACCAACAAACATGTTTGGAAGGTCTAAACACTATAGCCTCTGATCAGAGAGTGAAGAACGACTTAACGTCATTGCTCTCAGATGACATGAAGCTTCATAGTGTCACCCTAGCCTTGTTCAAGAAAGGTTGGGTTCCTAAAAGTAAAATAAGAACATCATGGCCACAAAATGGCAAGCATTTGAATTTCAAAAATGGTCATTTGCCATTGAAGATGTCAAATAAAGCACGTGCTATTTATGATTCTGCTAGACGCAATGGGAGAAAACTACTTCAAACTAATACTAATGAAGATAGTGTTGTGGTGAGTGATATTGTCGTTGTTAGTCAAGATGGAAGTGGAAACTTTACCACTATCAATGACGCGGTAGCTGCTGCACCAAATAACACGGTTGCTAGTGATGGATACTTCTTCATATTTATCACCAAAGGTGTGTATCAAGAGTATGTATCTATACCCAAAAACAAAAAGTACTTGATGATGGTTGGAGAGGGAATCAATCAAACAGTTATCACTGGAGACCACAATGTTGTTGATGGTTTCACAACATTCAATTCGGCTACATTTGGTAAGCAAATTTAATACTTGGAGTTGGACATATTCAATATACTATTACAAAAAAATGATATTTCATCATAAATTGTTTAGCTATATACGGATTAAAAAATTCAGCAGCCAATTGAAGATTTTTGGACCACAATGTTGAAGATAATCTATAGTTTAAAAATTTCACTAATTTGATGTTTTTTGTGAATTTTCAGCCGTAGTTGGTCAAGGGTTCGTAGCAGTTAACATAACATTTGGCAACACCGCTGGACCAAGCAAACACCAAGCAGTTGCAATGAGAAGTGGAGCAGATATGTCCACCTTCTATAGTTGTAGCTTTGAAGGGTATCAGGACACATTGTATACACATTCTCTAAGGCAGTTTTATCGAGAATGCGATATATACGGAACAGTTGACTTCATATTTGGAAACGGTGCAGTTGTTTTGCAAAATTGTAACATTTATCCTCGCCTTCCGCTAAGTGGACAATTCAATTCCATCACAGCTCAAGGTCGAACTGACCCAAATCAAAACACTGGAACTTCTATACAAAATGCAACTATTAAAGCAGCTGATGATTTGGCTCCGAATGTTGGAACGGTACAAACATACCTAGGGAGACCGTGGAAAGAATATTCAAGGACAGTTTTTATGCAATCTTTCATGGACAGTTTCATAAACCCTGCTGGTTGGCATGAATGGAATGGTGATTTTGCGTTGAATACTTTGTACTATGCAGAATACGGCAACAGAGGAGCGGGTTCAAGCATCGTGAATCGAGTGACATGGTCTGGTTATCATGTTATTGGTGCTACCGATGCAGCCAATTTCACGGTATCCAATTTCTTGAGTGGTGATGATTGGATTCCTCAAACTGGTGTTCCATACTCTAGTGGATTGATATAG
Protein sequence

>MS.gene005575.t1

MALKHKTIAYLLSILFFVSFHVANGVPPETICSYTVNPTYCKNVFANQNGNIYDYGKISIRKSLSQSRKFMNSIDSQFQGGSSLSQSTIRALEDCRFLAELSFEYLSNTYTTTNQSSNVLPTSQAEDFETFLSAVLTNQQTCLEGLNTIASDQRVKNDLTSLLSDDMKLHSVTLALFKKGWVPKSKIRTSWPQNGKHLNFKNGHLPLKMSNKARAIYDSARRNGRKLLQTNTNEDSVVVSDIVVVSQDGSGNFTTINDAVAAAPNNTVASDGYFFIFITKGVYQEYVSIPKNKKYLMMVGEGINQTVITGDHNVVDGFTTFNSATFAVVGQGFVAVNITFGNTAGPSKHQAVAMRSGADMSTFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNGAVVLQNCNIYPRLPLSGQFNSITAQGRTDPNQNTGTSIQNATIKAADDLAPNVGTVQTYLGRPWKEYSRTVFMQSFMDSFINPAGWHEWNGDFALNTLYYAEYGNRGAGSSIVNRVTWSGYHVIGATDAANFTVSNFLSGDDWIPQTGVPYSSGLI