AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41164

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41164.t1 MTR_4g063975 98.450 258 4 0 1 258 1 258 0.0 525
MS.gene41164.t1 MTR_2g097030 87.209 258 33 0 1 258 3 260 7.05e-175 482
MS.gene41164.t1 MTR_5g079950 82.661 248 43 0 11 258 12 259 2.63e-161 447
MS.gene41164.t1 MTR_7g061720 82.171 258 45 1 2 258 1 258 5.96e-161 447
MS.gene41164.t1 MTR_5g041700 66.800 250 81 1 8 257 1 248 1.62e-124 354
MS.gene41164.t1 MTR_4g081950 65.217 253 81 3 2 253 34 280 1.34e-122 350
MS.gene41164.t1 MTR_3g094060 64.314 255 86 3 5 256 1 253 5.56e-122 348
MS.gene41164.t1 MTR_3g094040 64.173 254 86 3 5 256 3 253 7.22e-122 347
MS.gene41164.t1 MTR_5g075320 63.780 254 87 3 5 256 1 251 4.22e-120 343
MS.gene41164.t1 MTR_3g435430 70.222 225 65 1 32 256 25 247 9.52e-120 342
MS.gene41164.t1 MTR_3g466760 63.281 256 92 1 1 256 7 260 3.80e-117 336
MS.gene41164.t1 MTR_3g466790 63.424 257 90 3 1 256 1 254 2.03e-116 334
MS.gene41164.t1 MTR_7g011060 63.265 245 80 2 12 256 6 240 9.97e-115 329
MS.gene41164.t1 MTR_7g111010 57.312 253 100 4 2 251 1 248 7.82e-107 310
MS.gene41164.t1 MTR_1g085460 61.157 242 86 4 16 254 18 254 1.30e-106 309
MS.gene41164.t1 MTR_6g012520 62.698 252 84 2 5 256 1 242 3.74e-105 305
MS.gene41164.t1 MTR_1g026020 57.422 256 103 3 1 254 1 252 7.24e-105 305
MS.gene41164.t1 MTR_7g110990 62.780 223 79 3 32 251 12 233 2.22e-103 300
MS.gene41164.t1 MTR_4g124340 59.471 227 89 2 30 255 59 283 7.91e-103 300
MS.gene41164.t1 MTR_8g464350 55.061 247 106 2 5 251 7 248 1.70e-100 293
MS.gene41164.t1 MTR_7g013150 55.738 244 105 2 11 254 10 250 5.15e-98 287
MS.gene41164.t1 MTR_8g058990 57.207 222 93 1 32 253 30 249 7.77e-95 279
MS.gene41164.t1 MTR_7g013140 57.399 223 93 1 32 254 28 248 4.86e-94 277
MS.gene41164.t1 MTR_7g013120 52.653 245 111 3 11 254 10 250 1.36e-93 276
MS.gene41164.t1 MTR_5g011210 50.394 254 123 3 6 257 11 263 1.59e-92 273
MS.gene41164.t1 MTR_4g102450 50.394 254 123 3 6 257 10 262 1.16e-91 271
MS.gene41164.t1 MTR_0040s0140 47.287 258 131 2 1 256 2 256 1.17e-88 263
MS.gene41164.t1 MTR_5g076950 55.310 226 100 1 31 256 40 264 7.48e-88 262
MS.gene41164.t1 MTR_2g090255 46.341 246 131 1 12 256 7 252 5.53e-83 249
MS.gene41164.t1 MTR_3g064510 52.340 235 103 5 31 257 52 285 1.41e-79 241
MS.gene41164.t1 MTR_0008s0120 59.091 110 45 0 144 253 86 195 3.96e-42 143
MS.gene41164.t1 MTR_0008s0120 56.471 85 33 2 5 88 7 88 2.92e-24 96.7
MS.gene41164.t1 MTR_6g012500 42.857 175 84 4 98 256 106 280 1.93e-40 141
MS.gene41164.t1 MTR_2g090270 30.041 243 144 9 24 254 27 255 5.82e-25 100
MS.gene41164.t1 MTR_2g090265 28.947 266 160 11 4 254 2 253 1.93e-23 95.9
MS.gene41164.t1 MTR_0122s0020 31.780 236 135 11 31 254 40 261 9.38e-23 94.4
MS.gene41164.t1 MTR_4g028640 29.237 236 132 11 32 251 42 258 4.14e-18 81.6
MS.gene41164.t1 MTR_0118s0070 28.571 231 135 12 37 251 53 269 1.86e-16 77.4
MS.gene41164.t1 MTR_0809s0010 28.512 242 137 13 27 251 42 264 2.42e-15 73.9
MS.gene41164.t1 MTR_0122s0020 33.838 198 97 12 21 198 21 204 7.79e-15 71.6
MS.gene41164.t1 MTR_5g013440 25.991 227 151 9 35 254 32 248 1.84e-13 68.6
MS.gene41164.t1 MTR_4g099370 25.751 233 152 10 28 251 24 244 3.81e-13 67.8
MS.gene41164.t1 MTR_1g085365 53.571 56 26 0 201 256 4 59 4.19e-13 63.2
MS.gene41164.t1 MTR_4g075790 28.378 222 124 9 43 248 32 234 4.79e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41164.t1 AT2G39700 82.879 257 44 0 2 258 1 257 3.97e-164 454
MS.gene41164.t1 AT2G28950 82.490 257 45 0 2 258 1 257 4.14e-156 434
MS.gene41164.t1 AT2G37640 79.447 253 52 0 6 258 10 262 3.81e-154 429
MS.gene41164.t1 AT3G55500 78.346 254 55 0 5 258 7 260 5.09e-154 429
MS.gene41164.t1 AT5G02260 74.419 258 66 0 1 258 1 258 8.36e-152 424
MS.gene41164.t1 AT2G37640 82.381 210 37 0 49 258 28 237 1.97e-132 374
MS.gene41164.t1 AT1G69530 66.798 253 81 2 5 257 1 250 6.81e-125 355
MS.gene41164.t1 AT1G69530 66.798 253 81 2 5 257 1 250 6.81e-125 355
MS.gene41164.t1 AT1G69530 66.403 253 82 2 5 257 1 250 2.60e-123 352
MS.gene41164.t1 AT2G03090 66.935 248 79 2 11 257 8 253 4.62e-122 348
MS.gene41164.t1 AT5G56320 64.844 256 83 3 5 257 1 252 1.07e-120 345
MS.gene41164.t1 AT5G56320 64.844 256 83 3 5 257 1 252 1.07e-120 345
MS.gene41164.t1 AT5G56320 64.844 256 83 3 5 257 1 252 6.84e-120 344
MS.gene41164.t1 AT1G26770 66.403 253 81 2 5 257 1 249 9.40e-118 337
MS.gene41164.t1 AT1G26770 66.142 254 82 2 4 257 10 259 1.29e-117 337
MS.gene41164.t1 AT5G05290 62.109 256 93 3 2 256 3 255 4.97e-115 330
MS.gene41164.t1 AT2G40610 62.698 252 88 3 6 256 7 253 3.22e-114 328
MS.gene41164.t1 AT3G29030 65.929 226 68 2 32 257 38 254 3.93e-110 318
MS.gene41164.t1 AT1G69530 61.660 253 64 3 5 257 1 220 4.74e-109 314
MS.gene41164.t1 AT1G69530 61.265 253 65 3 5 257 1 220 1.26e-107 311
MS.gene41164.t1 AT4G01630 61.066 244 87 5 11 251 11 249 5.74e-106 307
MS.gene41164.t1 AT1G20190 61.538 221 82 2 34 252 28 247 1.56e-97 286
MS.gene41164.t1 AT5G39290 50.000 278 111 5 1 254 3 276 1.30e-95 282
MS.gene41164.t1 AT5G39270 50.000 278 111 4 1 254 3 276 4.23e-95 281
MS.gene41164.t1 AT1G62980 52.800 250 116 2 6 254 5 253 9.64e-93 274
MS.gene41164.t1 AT5G39280 55.157 223 96 2 32 254 54 272 1.31e-89 266
MS.gene41164.t1 AT1G12560 51.807 249 116 4 11 257 16 262 1.86e-89 266
MS.gene41164.t1 AT5G39300 47.636 275 119 5 1 254 3 273 7.35e-89 265
MS.gene41164.t1 AT5G39310 54.911 224 96 3 32 254 90 309 2.30e-87 262
MS.gene41164.t1 AT3G03220 53.509 228 105 1 31 258 39 265 3.89e-86 257
MS.gene41164.t1 AT4G38210 45.565 248 133 1 12 257 8 255 1.14e-80 243
MS.gene41164.t1 AT5G39260 47.191 267 120 6 1 255 3 260 1.81e-76 233
MS.gene41164.t1 AT1G20190 57.979 188 76 2 67 252 2 188 2.90e-76 229
MS.gene41164.t1 AT3G15370 46.512 258 123 7 1 254 1 247 9.12e-76 231
MS.gene41164.t1 AT3G15370 41.667 288 123 8 1 254 1 277 3.30e-69 215
MS.gene41164.t1 AT4G28250 30.705 241 138 11 28 254 32 257 9.76e-24 97.1
MS.gene41164.t1 AT2G20750 29.114 237 144 9 31 254 39 264 7.88e-22 92.0
MS.gene41164.t1 AT1G65680 30.126 239 138 11 28 251 43 267 4.08e-20 87.4
MS.gene41164.t1 AT4G28250 29.583 240 136 11 28 254 32 251 5.68e-20 86.7
MS.gene41164.t1 AT2G20750 32.386 176 97 7 31 195 39 203 3.50e-16 75.5
MS.gene41164.t1 AT4G28250 32.240 183 95 8 28 200 32 195 1.16e-15 73.9
MS.gene41164.t1 AT4G28250 32.584 178 91 8 28 195 32 190 7.20e-15 71.6
MS.gene41164.t1 AT3G60570 23.774 265 172 9 9 258 10 259 3.02e-13 68.2
MS.gene41164.t1 AT4G17030 25.000 212 138 7 3 209 4 199 5.62e-13 67.4
MS.gene41164.t1 AT3G45970 25.000 212 137 9 51 251 15 215 5.68e-12 64.3

Find 68 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCAATGTAGAGCATTT+TGG 0.215132 4.1:-36448232 None:intergenic
GACAAAGAGTGGTGTCATTC+TGG 0.235057 4.1:+36448756 MS.gene41164:CDS
TCACTTGGTTGAGCAAAATT+AGG 0.253485 4.1:-36448819 None:intergenic
GCTCCTCCTAGTTGGCAATT+TGG 0.267981 4.1:+36450742 MS.gene41164:CDS
GATGCTCATGCTACTTTCTA+TGG 0.312345 4.1:+36448324 MS.gene41164:CDS
TTTGCTCAACCAAGTGATAA+TGG 0.336925 4.1:+36448825 MS.gene41164:CDS
TATGGTGGATCTGATGCTTC+TGG 0.339901 4.1:+36448342 MS.gene41164:CDS
GCCAGTTCTGACCCCAGTTT+CGG 0.341334 4.1:-36450636 None:intergenic
GGATGCCACTGAGCCGAAAC+TGG 0.348922 4.1:+36450623 MS.gene41164:CDS
ATTGTTGAAGCTAGAATTCC+TGG 0.372131 4.1:+36448276 MS.gene41164:CDS
CAATTTGGTCAAACTTTCAC+TGG 0.389947 4.1:+36450757 MS.gene41164:CDS
ACTAACCTGCGGTAAGCAAC+AGG 0.390868 4.1:-36448933 None:intergenic
CAGTGGCATCCACTCGGTTC+GGG 0.402414 4.1:-36450611 None:intergenic
TCAGTGGCATCCACTCGGTT+CGG 0.423453 4.1:-36450612 None:intergenic
ACCGTAGATCTTCAACATCT+TGG 0.445386 4.1:+36450713 MS.gene41164:CDS
GCCGAAACTGGGGTCAGAAC+TGG 0.448238 4.1:+36450635 MS.gene41164:CDS
ACAAAGAGTGGTGTCATTCT+GGG 0.452005 4.1:+36448757 MS.gene41164:CDS
CACCAACCACCATTATCACT+TGG 0.453463 4.1:-36448834 None:intergenic
CGGATTCCGTTACTTTAACC+TGG 0.456362 4.1:+36450531 MS.gene41164:CDS
GTTTGACCAAATTGCCAACT+AGG 0.460335 4.1:-36450748 None:intergenic
AGAATTCCTGGTGTTTACTC+TGG 0.487043 4.1:+36448288 MS.gene41164:CDS
GGAACATTGCTCCTCCTAGT+TGG 0.491170 4.1:+36450734 MS.gene41164:CDS
GTAGCAGTAATGAAAATTGA+AGG 0.495390 4.1:-36448783 None:intergenic
TCTGATGCTTCTGGCACCAT+GGG 0.503667 4.1:+36448351 MS.gene41164:CDS
TTAGGAGGAGCATGTGGGTA+CGG 0.517583 4.1:+36448627 MS.gene41164:intron
GTGTTTACTCTGGTGGTCAA+TGG 0.519210 4.1:+36448298 MS.gene41164:CDS
ATTCCTGGTGTTTACTCTGG+TGG 0.519949 4.1:+36448291 MS.gene41164:CDS
TTGTCCCTGTTGCTTACCGC+AGG 0.522475 4.1:+36448928 MS.gene41164:CDS
TCAGCAATCTTAAGAAACAT+AGG 0.524078 4.1:-36448891 None:intergenic
AATTTGTACAGTCAAGGTTA+CGG 0.526954 4.1:+36448651 MS.gene41164:CDS
ATTAGCAACGTAGCAGGTGC+TGG 0.528629 4.1:+36450559 MS.gene41164:CDS
ATCTGATGCTTCTGGCACCA+TGG 0.529188 4.1:+36448350 MS.gene41164:CDS
GTGCTAATTAGCAACGTAGC+AGG 0.532373 4.1:+36450553 MS.gene41164:CDS
CTACGTTGCTAATTAGCACC+AGG 0.535801 4.1:-36450549 None:intergenic
AAAGTAACGGAATCCGTTCA+CGG 0.539938 4.1:-36450524 None:intergenic
TCCAAGATGTTGAAGATCTA+CGG 0.544252 4.1:-36450714 None:intergenic
GATGCCACTGAGCCGAAACT+GGG 0.544292 4.1:+36450624 MS.gene41164:CDS
GGAATAAGATTCACCGTGAA+CGG 0.547347 4.1:+36450511 MS.gene41164:CDS
ATCGTGCGCGCCTACGTGAA+AGG 0.554876 4.1:+36450586 MS.gene41164:CDS
TACGGAAATTTGTACAGTCA+AGG 0.556701 4.1:+36448645 MS.gene41164:CDS
ATTCCACCACGCTTTCTGCA+TGG 0.558295 4.1:-36450493 None:intergenic
AAACACGAAAGGACAAAGCC+TGG 0.564825 4.1:-36450681 None:intergenic
AAGATTGCTGAGTATCGTGC+TGG 0.570660 4.1:+36448903 MS.gene41164:CDS
AGCACTGCTTTGTTCAACAA+TGG 0.578791 4.1:+36448693 MS.gene41164:CDS
GGTTCGGGTTCCTTTCACGT+AGG 0.581009 4.1:-36450596 None:intergenic
TCGGCTCAGTGGCATCCACT+CGG 0.585195 4.1:-36450617 None:intergenic
TTCAACAATGGTTTAAGCTG+TGG 0.599006 4.1:+36448705 MS.gene41164:CDS
CTTGGTTGAGCAAAATTAGG+AGG 0.603163 4.1:-36448816 None:intergenic
TTAGCACCAGGTTAAAGTAA+CGG 0.608652 4.1:-36450537 None:intergenic
GGCATAGCAAGATCAAAATG+AGG 0.611060 4.1:-36448870 None:intergenic
TGACCACCAGAGTAAACACC+AGG 0.616969 4.1:-36448294 None:intergenic
GCTCATGCTACTTTCTATGG+TGG 0.618802 4.1:+36448327 MS.gene41164:CDS
TGACCCCAGTTTCGGCTCAG+TGG 0.625730 4.1:-36450628 None:intergenic
CTGGCCCACTAAAACCGCGT+TGG 0.630033 4.1:-36450662 None:intergenic
GCAAGATCAAAATGAGGACG+AGG 0.630560 4.1:-36448864 None:intergenic
GTCACTGCTTGAAACACGAA+AGG 0.633040 4.1:-36450692 None:intergenic
TGACCAAATTGCCAACTAGG+AGG 0.638092 4.1:-36450745 None:intergenic
AGGGTGCCATGCAGAAAGCG+TGG 0.647035 4.1:+36450487 MS.gene41164:intron
AACCAAGTGATAATGGTGGT+TGG 0.649043 4.1:+36448832 MS.gene41164:CDS
AGATCAAAATGAGGACGAGG+AGG 0.649689 4.1:-36448861 None:intergenic
TGAAAGGAACCCGAACCGAG+TGG 0.653524 4.1:+36450602 MS.gene41164:CDS
GATCAAAATGAGGACGAGGA+GGG 0.663864 4.1:-36448860 None:intergenic
GTGCCATGCAGAAAGCGTGG+TGG 0.676430 4.1:+36450490 MS.gene41164:CDS
ATGCCACTGAGCCGAAACTG+GGG 0.678002 4.1:+36450625 MS.gene41164:CDS
AGTGTGCAAATGACAAAGAG+TGG 0.680419 4.1:+36448745 MS.gene41164:CDS
CTAACCTGCGGTAAGCAACA+GGG 0.680825 4.1:-36448932 None:intergenic
GCTCAACCAAGTGATAATGG+TGG 0.719451 4.1:+36448828 MS.gene41164:CDS
TCAGAACTGGCAATCCAACG+CGG 0.772854 4.1:+36450648 MS.gene41164:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG + chr4.1:36449678-36449697 MS.gene41164:intron 10.0%
!! TAAGAAAAATGTATAAATAA+AGG + chr4.1:36449677-36449696 MS.gene41164:intron 10.0%
!! TATACTACTAAAAAAAATTA+AGG - chr4.1:36449428-36449447 None:intergenic 10.0%
!!! AAGTTTATTTTATTTTACTT+TGG + chr4.1:36449169-36449188 MS.gene41164:intron 10.0%
!!! ACATATATAATTTTTTTTCA+AGG - chr4.1:36450094-36450113 None:intergenic 10.0%
!!! ATTTTATCTTTAAGTTATTT+AGG + chr4.1:36449141-36449160 MS.gene41164:intron 10.0%
!!! TTAATTATCCAATTTTTATA+TGG - chr4.1:36450315-36450334 None:intergenic 10.0%
!! AAAGTAGAATATATTGTATT+GGG + chr4.1:36449702-36449721 MS.gene41164:intron 15.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr4.1:36449679-36449698 MS.gene41164:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr4.1:36449743-36449762 MS.gene41164:intron 15.0%
!! CTATCTATATTAAATAGAAA+TGG - chr4.1:36450280-36450299 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr4.1:36449742-36449761 MS.gene41164:intron 15.0%
!!! ATATATTATTGACTTTTCTT+TGG + chr4.1:36449483-36449502 MS.gene41164:intron 15.0%
!!! ATGTTTTTTTAGGTTTAATT+AGG - chr4.1:36448565-36448584 None:intergenic 15.0%
!!! TAAAAATAATATAAAGTACC+AGG + chr4.1:36449390-36449409 MS.gene41164:intron 15.0%
!!! TTTATGTTAATTTTTTGTGT+TGG + chr4.1:36448599-36448618 MS.gene41164:intron 15.0%
!!! TTTTCTTTTTCATTAATTGA+GGG - chr4.1:36449456-36449475 None:intergenic 15.0%
!! AAAAGAAGAAGAAAAATTAG+AGG + chr4.1:36449353-36449372 MS.gene41164:intron 20.0%
!! AAATTGGAATTAATTATGGT+AGG + chr4.1:36450368-36450387 MS.gene41164:intron 20.0%
!! AATTGGAATTAATTATGGTA+GGG + chr4.1:36450369-36450388 MS.gene41164:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + chr4.1:36449775-36449794 MS.gene41164:intron 20.0%
!! GAAAGTAGAATATATTGTAT+TGG + chr4.1:36449701-36449720 MS.gene41164:intron 20.0%
!! GTAGAAATTGGAATTAATTA+TGG + chr4.1:36450364-36450383 MS.gene41164:intron 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG + chr4.1:36449776-36449795 MS.gene41164:intron 20.0%
!! TATAGTTAAGAAATTAGTTG+TGG + chr4.1:36449517-36449536 MS.gene41164:intron 20.0%
!! TTAAGGTTAAATTATATTCC+TGG - chr4.1:36449411-36449430 None:intergenic 20.0%
!!! ATTTTGTTGATATTTACACA+GGG + chr4.1:36450468-36450487 MS.gene41164:intron 20.0%
!!! CTTTTCTTTTTCATTAATTG+AGG - chr4.1:36449457-36449476 None:intergenic 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr4.1:36449812-36449831 MS.gene41164:intron 20.0%
!!! GTAACATTTCATGTTTTTTT+AGG - chr4.1:36448575-36448594 None:intergenic 20.0%
!!! TATTTTGTTGATATTTACAC+AGG + chr4.1:36450467-36450486 MS.gene41164:intron 20.0%
!!! TTTTTGTTCAAGTAATGTAT+GGG + chr4.1:36448968-36448987 MS.gene41164:intron 20.0%
!!! TTTTTTGTTCAAGTAATGTA+TGG + chr4.1:36448967-36448986 MS.gene41164:intron 20.0%
! AAAATGAAGTAGTAAAAGAG+CGG - chr4.1:36449066-36449085 None:intergenic 25.0%
! AATATTTAGGTCTCTTAACA+AGG + chr4.1:36449219-36449238 MS.gene41164:intron 25.0%
! AATCACTTTGACATATAGTT+AGG + chr4.1:36448440-36448459 MS.gene41164:intron 25.0%
! AGATATGAATGAAGTAACTA+GGG - chr4.1:36449599-36449618 None:intergenic 25.0%
! ATCACTTTGACATATAGTTA+GGG + chr4.1:36448441-36448460 MS.gene41164:intron 25.0%
! ATGGAAAGAATGAAAAAGAA+AGG - chr4.1:36450118-36450137 None:intergenic 25.0%
! GATACAAGTGGTAAATATTT+AGG + chr4.1:36449206-36449225 MS.gene41164:intron 25.0%
! TAAAATATGTGACACTAACA+TGG - chr4.1:36450137-36450156 None:intergenic 25.0%
! TATCCACATGTTTGTATTTA+CGG - chr4.1:36448516-36448535 None:intergenic 25.0%
!! AGTGTCACATATTTTACTTT+TGG + chr4.1:36450141-36450160 MS.gene41164:intron 25.0%
!! TGAAATGGGTCAATTATTTT+GGG + chr4.1:36449790-36449809 MS.gene41164:intron 25.0%
!! TTGAAATGGGTCAATTATTT+TGG + chr4.1:36449789-36449808 MS.gene41164:intron 25.0%
!! TTTCACTGGAAAGAATTTTA+GGG + chr4.1:36450771-36450790 MS.gene41164:CDS 25.0%
!! TTTTGTTCAAGTAATGTATG+GGG + chr4.1:36448969-36448988 MS.gene41164:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr4.1:36449811-36449830 MS.gene41164:intron 25.0%
!!! TTTTTTGTGTTGGTGTTTTT+AGG + chr4.1:36448609-36448628 MS.gene41164:intron 25.0%
AATTTGTACAGTCAAGGTTA+CGG + chr4.1:36448651-36448670 MS.gene41164:CDS 30.0%
AGAAAATATGAGGGCAAAAA+GGG - chr4.1:36448398-36448417 None:intergenic 30.0%
ATAGACAGATTATTACATGC+TGG - chr4.1:36449018-36449037 None:intergenic 30.0%
CAGATATGAATGAAGTAACT+AGG - chr4.1:36449600-36449619 None:intergenic 30.0%
CGACTCAACCATATAAAAAT+TGG + chr4.1:36450304-36450323 MS.gene41164:intron 30.0%
GACATAATCTCCATGTATTA+GGG - chr4.1:36448486-36448505 None:intergenic 30.0%
GTAGCAGTAATGAAAATTGA+AGG - chr4.1:36448786-36448805 None:intergenic 30.0%
GTTAAGAAATTAGTTGTGGT+AGG + chr4.1:36449521-36449540 MS.gene41164:intron 30.0%
TAGACAGATTATTACATGCT+GGG - chr4.1:36449017-36449036 None:intergenic 30.0%
TAGCTTAAAACCCTAATACA+TGG + chr4.1:36448473-36448492 MS.gene41164:intron 30.0%
TCAGCAATCTTAAGAAACAT+AGG - chr4.1:36448894-36448913 None:intergenic 30.0%
TCTTAAGTTTGTGATACAAG+TGG + chr4.1:36449194-36449213 MS.gene41164:intron 30.0%
TGACATAATCTCCATGTATT+AGG - chr4.1:36448487-36448506 None:intergenic 30.0%
TGTACGGAAAAAGTAGAAAT+TGG + chr4.1:36450352-36450371 MS.gene41164:intron 30.0%
! AGAAAAGTATAACTAACCTG+CGG - chr4.1:36448947-36448966 None:intergenic 30.0%
! CAATTTTTGTACGAAGAACA+AGG - chr4.1:36450024-36450043 None:intergenic 30.0%
! CTTTCACTGGAAAGAATTTT+AGG + chr4.1:36450770-36450789 MS.gene41164:CDS 30.0%
AAATTTAGGTACGTTGACGT+AGG - chr4.1:36450210-36450229 None:intergenic 35.0%
AATAAGTGTGAAATGAGCCA+AGG - chr4.1:36449658-36449677 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr4.1:36449825-36449844 MS.gene41164:intron 35.0%
ATAAGTGTGAAATGAGCCAA+GGG - chr4.1:36449657-36449676 None:intergenic 35.0%
ATTGTTGAAGCTAGAATTCC+TGG + chr4.1:36448276-36448295 MS.gene41164:CDS 35.0%
CAATTTGGTCAAACTTTCAC+TGG + chr4.1:36450757-36450776 MS.gene41164:CDS 35.0%
CAGAAAATATGAGGGCAAAA+AGG - chr4.1:36448399-36448418 None:intergenic 35.0%
CCTCAATCCAGAAAATATGA+GGG - chr4.1:36448407-36448426 None:intergenic 35.0%
GTGAAGTGATATAGACTGTA+CGG + chr4.1:36450336-36450355 MS.gene41164:intron 35.0%
TACGGAAATTTGTACAGTCA+AGG + chr4.1:36448645-36448664 MS.gene41164:CDS 35.0%
TAGTTGCTAGTCAAATGTGA+TGG + chr4.1:36449859-36449878 MS.gene41164:intron 35.0%
TCACCGTAAATACAAACATG+TGG + chr4.1:36448510-36448529 MS.gene41164:intron 35.0%
TCACTTGGTTGAGCAAAATT+AGG - chr4.1:36448822-36448841 None:intergenic 35.0%
TCCAAGATGTTGAAGATCTA+CGG - chr4.1:36450717-36450736 None:intergenic 35.0%
TTAGCACCAGGTTAAAGTAA+CGG - chr4.1:36450540-36450559 None:intergenic 35.0%
TTCAACAATGGTTTAAGCTG+TGG + chr4.1:36448705-36448724 MS.gene41164:CDS 35.0%
TTCATTAACTGTGATGAGAC+TGG - chr4.1:36449550-36449569 None:intergenic 35.0%
TTTGCTCAACCAAGTGATAA+TGG + chr4.1:36448825-36448844 MS.gene41164:CDS 35.0%
! CCTCATATTTTCTGGATTGA+GGG + chr4.1:36448405-36448424 MS.gene41164:intron 35.0%
! CTTTTTGCCCTCATATTTTC+TGG + chr4.1:36448397-36448416 MS.gene41164:intron 35.0%
!! GAAAGCAATGTAGAGCATTT+TGG - chr4.1:36448235-36448254 None:intergenic 35.0%
!! GGTAATTTGTTACATACCCA+TGG - chr4.1:36448370-36448389 None:intergenic 35.0%
!! TGATGGTGTTTTCGACTTTT+TGG + chr4.1:36449876-36449895 MS.gene41164:intron 35.0%
!!! TTTGTGTTGGTGTTTTTAGG+AGG + chr4.1:36448612-36448631 MS.gene41164:intron 35.0%
AAGTAATGTATGGGGTTTCC+GGG + chr4.1:36448977-36448996 MS.gene41164:intron 40.0%
ACCGTAGATCTTCAACATCT+TGG + chr4.1:36450713-36450732 MS.gene41164:CDS 40.0%
AGAATTCCTGGTGTTTACTC+TGG + chr4.1:36448288-36448307 MS.gene41164:CDS 40.0%
AGATTATTACATGCTGGGCT+TGG - chr4.1:36449012-36449031 None:intergenic 40.0%
AGCCAAGGGGTCAATTAATT+CGG - chr4.1:36449643-36449662 None:intergenic 40.0%
AGTGTGCAAATGACAAAGAG+TGG + chr4.1:36448745-36448764 MS.gene41164:CDS 40.0%
ATGAGGGCAAAAAGGGAAAT+GGG - chr4.1:36448391-36448410 None:intergenic 40.0%
ATTATTACATGCTGGGCTTG+GGG - chr4.1:36449010-36449029 None:intergenic 40.0%
CAAGTAATGTATGGGGTTTC+CGG + chr4.1:36448976-36448995 MS.gene41164:intron 40.0%
CCCTCAATCCAGAAAATATG+AGG - chr4.1:36448408-36448427 None:intergenic 40.0%
CTTGGTTGAGCAAAATTAGG+AGG - chr4.1:36448819-36448838 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr4.1:36449826-36449845 MS.gene41164:intron 40.0%
GATTATTACATGCTGGGCTT+GGG - chr4.1:36449011-36449030 None:intergenic 40.0%
GGAATAAGATTCACCGTGAA+CGG + chr4.1:36450511-36450530 MS.gene41164:CDS 40.0%
GGCATAGCAAGATCAAAATG+AGG - chr4.1:36448873-36448892 None:intergenic 40.0%
GTGTGTCTGATTCATGAAGT+GGG + chr4.1:36449931-36449950 MS.gene41164:intron 40.0%
GTTTGACCAAATTGCCAACT+AGG - chr4.1:36450751-36450770 None:intergenic 40.0%
TAAGTGTGAAATGAGCCAAG+GGG - chr4.1:36449656-36449675 None:intergenic 40.0%
TATGAGGGCAAAAAGGGAAA+TGG - chr4.1:36448392-36448411 None:intergenic 40.0%
TGTGTCTGATTCATGAAGTG+GGG + chr4.1:36449932-36449951 MS.gene41164:intron 40.0%
TGTGTGTCTGATTCATGAAG+TGG + chr4.1:36449930-36449949 MS.gene41164:intron 40.0%
TTCCAACTGAGTTAAGCTCT+TGG + chr4.1:36449037-36449056 MS.gene41164:intron 40.0%
TTCCAAGAGCTTAACTCAGT+TGG - chr4.1:36449042-36449061 None:intergenic 40.0%
! AAAGTAACGGAATCCGTTCA+CGG - chr4.1:36450527-36450546 None:intergenic 40.0%
! AGCACTGCTTTGTTCAACAA+TGG + chr4.1:36448693-36448712 MS.gene41164:CDS 40.0%
! CCCTCATATTTTCTGGATTG+AGG + chr4.1:36448404-36448423 MS.gene41164:intron 40.0%
!! AACCAAGTGATAATGGTGGT+TGG + chr4.1:36448832-36448851 MS.gene41164:CDS 40.0%
!! ACAAAGAGTGGTGTCATTCT+GGG + chr4.1:36448757-36448776 MS.gene41164:CDS 40.0%
!! GATGCTCATGCTACTTTCTA+TGG + chr4.1:36448324-36448343 MS.gene41164:CDS 40.0%
!!! TGTTTTTAGGAGGAGCATGT+GGG + chr4.1:36448622-36448641 MS.gene41164:intron 40.0%
AAACACGAAAGGACAAAGCC+TGG - chr4.1:36450684-36450703 None:intergenic 45.0%
AGATCAAAATGAGGACGAGG+AGG - chr4.1:36448864-36448883 None:intergenic 45.0%
AGGGGTCAATTAATTCGGGA+CGG - chr4.1:36449638-36449657 None:intergenic 45.0%
AGTAATGTATGGGGTTTCCG+GGG + chr4.1:36448978-36448997 MS.gene41164:intron 45.0%
ATTCCTGGTGTTTACTCTGG+TGG + chr4.1:36448291-36448310 MS.gene41164:CDS 45.0%
CACCAACCACCATTATCACT+TGG - chr4.1:36448837-36448856 None:intergenic 45.0%
CTACGTTGCTAATTAGCACC+AGG - chr4.1:36450552-36450571 None:intergenic 45.0%
GATCAAAATGAGGACGAGGA+GGG - chr4.1:36448863-36448882 None:intergenic 45.0%
GCAAGATCAAAATGAGGACG+AGG - chr4.1:36448867-36448886 None:intergenic 45.0%
GCCAAGGGGTCAATTAATTC+GGG - chr4.1:36449642-36449661 None:intergenic 45.0%
GTCAATTAATTCGGGACGGA+GGG - chr4.1:36449634-36449653 None:intergenic 45.0%
GTCACTGCTTGAAACACGAA+AGG - chr4.1:36450695-36450714 None:intergenic 45.0%
GTGCTAATTAGCAACGTAGC+AGG + chr4.1:36450553-36450572 MS.gene41164:CDS 45.0%
GTGTTTACTCTGGTGGTCAA+TGG + chr4.1:36448298-36448317 MS.gene41164:CDS 45.0%
TCCCGAATTAATTGACCCCT+TGG + chr4.1:36449638-36449657 MS.gene41164:intron 45.0%
TGACCAAATTGCCAACTAGG+AGG - chr4.1:36450748-36450767 None:intergenic 45.0%
! AAGATTGCTGAGTATCGTGC+TGG + chr4.1:36448903-36448922 MS.gene41164:CDS 45.0%
! CGGATTCCGTTACTTTAACC+TGG + chr4.1:36450531-36450550 MS.gene41164:CDS 45.0%
!! AAGTGGGGTAGTGTTACTTC+GGG + chr4.1:36449947-36449966 MS.gene41164:intron 45.0%
!! CAATCCAACGCGGTTTTAGT+GGG + chr4.1:36450658-36450677 MS.gene41164:CDS 45.0%
!! GAAGTGGGGTAGTGTTACTT+CGG + chr4.1:36449946-36449965 MS.gene41164:intron 45.0%
!! GACAAAGAGTGGTGTCATTC+TGG + chr4.1:36448756-36448775 MS.gene41164:CDS 45.0%
!! GCTCAACCAAGTGATAATGG+TGG + chr4.1:36448828-36448847 MS.gene41164:CDS 45.0%
!! GCTCATGCTACTTTCTATGG+TGG + chr4.1:36448327-36448346 MS.gene41164:CDS 45.0%
!! GGTAGTGTTACTTCGGGTAA+AGG + chr4.1:36449953-36449972 MS.gene41164:intron 45.0%
!! TATGGTGGATCTGATGCTTC+TGG + chr4.1:36448342-36448361 MS.gene41164:CDS 45.0%
!!! GTGTTTTTAGGAGGAGCATG+TGG + chr4.1:36448621-36448640 MS.gene41164:intron 45.0%
!! TAAAAAAAAAATGAAAATTT+AGG - chr4.1:36450224-36450243 None:intergenic 5.0%
!!! ATTATTTTTAGATTTTATTT+TGG - chr4.1:36449380-36449399 None:intergenic 5.0%
!!! TTCATTTTTTTTTATTTTTT+TGG + chr4.1:36449078-36449097 MS.gene41164:intron 5.0%
ACTAACCTGCGGTAAGCAAC+AGG - chr4.1:36448936-36448955 None:intergenic 50.0%
ATCTGATGCTTCTGGCACCA+TGG + chr4.1:36448350-36448369 MS.gene41164:CDS 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr4.1:36449830-36449849 MS.gene41164:intron 50.0%
ATTAGCAACGTAGCAGGTGC+TGG + chr4.1:36450559-36450578 MS.gene41164:CDS 50.0%
ATTCCACCACGCTTTCTGCA+TGG - chr4.1:36450496-36450515 None:intergenic 50.0%
CTAACCTGCGGTAAGCAACA+GGG - chr4.1:36448935-36448954 None:intergenic 50.0%
GCTCCTCCTAGTTGGCAATT+TGG + chr4.1:36450742-36450761 MS.gene41164:CDS 50.0%
GGAACATTGCTCCTCCTAGT+TGG + chr4.1:36450734-36450753 MS.gene41164:CDS 50.0%
GGTCAATTAATTCGGGACGG+AGG - chr4.1:36449635-36449654 None:intergenic 50.0%
TCAGAACTGGCAATCCAACG+CGG + chr4.1:36450648-36450667 MS.gene41164:CDS 50.0%
TCTGATGCTTCTGGCACCAT+GGG + chr4.1:36448351-36448370 MS.gene41164:CDS 50.0%
TGACCACCAGAGTAAACACC+AGG - chr4.1:36448297-36448316 None:intergenic 50.0%
! TTAGGAGGAGCATGTGGGTA+CGG + chr4.1:36448627-36448646 MS.gene41164:intron 50.0%
!! GCAATCCAACGCGGTTTTAG+TGG + chr4.1:36450657-36450676 MS.gene41164:CDS 50.0%
ATGCCACTGAGCCGAAACTG+GGG + chr4.1:36450625-36450644 MS.gene41164:CDS 55.0%
GATGCCACTGAGCCGAAACT+GGG + chr4.1:36450624-36450643 MS.gene41164:CDS 55.0%
GTCAGTTATTCCGGGACGGA+GGG + chr4.1:36449834-36449853 MS.gene41164:intron 55.0%
TCAGTGGCATCCACTCGGTT+CGG - chr4.1:36450615-36450634 None:intergenic 55.0%
TGAAAGGAACCCGAACCGAG+TGG + chr4.1:36450602-36450621 MS.gene41164:CDS 55.0%
! GCCAGTTCTGACCCCAGTTT+CGG - chr4.1:36450639-36450658 None:intergenic 55.0%
! GGTTCGGGTTCCTTTCACGT+AGG - chr4.1:36450599-36450618 None:intergenic 55.0%
! TTGTCCCTGTTGCTTACCGC+AGG + chr4.1:36448928-36448947 MS.gene41164:CDS 55.0%
AGCAACTACTCCCTCCGTCC+CGG - chr4.1:36449847-36449866 None:intergenic 60.0%
ATCGTGCGCGCCTACGTGAA+AGG + chr4.1:36450586-36450605 MS.gene41164:CDS 60.0%
CAGTGGCATCCACTCGGTTC+GGG - chr4.1:36450614-36450633 None:intergenic 60.0%
CTGGCCCACTAAAACCGCGT+TGG - chr4.1:36450665-36450684 None:intergenic 60.0%
GCCGAAACTGGGGTCAGAAC+TGG + chr4.1:36450635-36450654 MS.gene41164:CDS 60.0%
GGATGCCACTGAGCCGAAAC+TGG + chr4.1:36450623-36450642 MS.gene41164:CDS 60.0%
GGTCAGTTATTCCGGGACGG+AGG + chr4.1:36449833-36449852 MS.gene41164:intron 60.0%
TCGGCTCAGTGGCATCCACT+CGG - chr4.1:36450620-36450639 None:intergenic 60.0%
TGACCCCAGTTTCGGCTCAG+TGG - chr4.1:36450631-36450650 None:intergenic 60.0%
! AGGGTGCCATGCAGAAAGCG+TGG + chr4.1:36450487-36450506 MS.gene41164:intron 60.0%
! GTGCCATGCAGAAAGCGTGG+TGG + chr4.1:36450490-36450509 MS.gene41164:CDS 60.0%
!! CAACGCGGTTTTAGTGGGCC+AGG + chr4.1:36450663-36450682 MS.gene41164:CDS 60.0%
TGGGCTTGGGGTTCGAGCCC+CGG - chr4.1:36448998-36449017 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 36448225 36450798 36448225 ID=MS.gene41164
chr4.1 mRNA 36448225 36450798 36448225 ID=MS.gene41164.t1;Parent=MS.gene41164
chr4.1 exon 36448225 36448372 36448225 ID=MS.gene41164.t1.exon1;Parent=MS.gene41164.t1
chr4.1 CDS 36448225 36448372 36448225 ID=cds.MS.gene41164.t1;Parent=MS.gene41164.t1
chr4.1 exon 36448631 36448949 36448631 ID=MS.gene41164.t1.exon2;Parent=MS.gene41164.t1
chr4.1 CDS 36448631 36448949 36448631 ID=cds.MS.gene41164.t1;Parent=MS.gene41164.t1
chr4.1 exon 36450489 36450798 36450489 ID=MS.gene41164.t1.exon3;Parent=MS.gene41164.t1
chr4.1 CDS 36450489 36450798 36450489 ID=cds.MS.gene41164.t1;Parent=MS.gene41164.t1
Gene Sequence

>MS.gene41164

ATGGTGGCCAAAATGCTCTACATTGCTTTCATAATTATCATTTCAATGTACATTGTTGAAGCTAGAATTCCTGGTGTTTACTCTGGTGGTCAATGGCAAGATGCTCATGCTACTTTCTATGGTGGATCTGATGCTTCTGGCACCATGGGTATGTAACAAATTACCCATTTCCCTTTTTGCCCTCATATTTTCTGGATTGAGGGTGAGTTTGACAAAATCACTTTGACATATAGTTAGGGTTACATGTATAGCTTAAAACCCTAATACATGGAGATTATGTCAAATTCACCGTAAATACAAACATGTGGATAAAATACATACAAATAATTACAATGTACCTAATTAAACCTAAAAAAACATGAAATGTTACTATGTTTATGTTAATTTTTTGTGTTGGTGTTTTTAGGAGGAGCATGTGGGTACGGAAATTTGTACAGTCAAGGTTACGGAGTGAACACAGCAGCATTAAGCACTGCTTTGTTCAACAATGGTTTAAGCTGTGGTGCTTGTTTTGAGATAAAGTGTGCAAATGACAAAGAGTGGTGTCATTCTGGGAGTCCTTCAATTTTCATTACTGCTACTAACTTTTGTCCTCCTAATTTTGCTCAACCAAGTGATAATGGTGGTTGGTGTAACCCTCCTCGTCCTCATTTTGATCTTGCTATGCCTATGTTTCTTAAGATTGCTGAGTATCGTGCTGGTATTGTCCCTGTTGCTTACCGCAGGTTAGTTATACTTTTCTTTTTTTGTTCAAGTAATGTATGGGGTTTCCGGGGCTCGAACCCCAAGCCCAGCATGTAATAATCTGTCTATTCCAACTGAGTTAAGCTCTTGGAAACCGCTCTTTTACTACTTCATTTTTTTTTATTTTTTTGGTATAAACTTCATTTTTAATTTTAGATTAATTAAGTTATACATTTTATCTTTAAGTTATTTAGGTCTCTAAGTTTATTTTATTTTACTTTGGTGTCTTAAGTTTGTGATACAAGTGGTAAATATTTAGGTCTCTTAACAAGGAAAAACATTTTGTTTCAATTTTTAAATAAAGTCTATAATGCTGTTAGAACAAAAAACAATTTATAGAGACAAAGTGTATAGTACTTGTCTGTCACTTAAATAACTAAAATGAAAAGAAGAAGAAAAATTAGAGGACCAAAATAAAATCTAAAAATAATATAAAGTACCAGGAATATAATTTAACCTTAATTTTTTTTAGTAGTATAGTACTCCCTCAATTAATGAAAAAGAAAAGTAGTTTATATATTATTGACTTTTCTTTGGAAAATGATGTTTATAGTTAAGAAATTAGTTGTGGTAGGAGTCCAGTCTCATCACAGTTAATGAAAAAAGAAAAGTAGCTTATATTATTGACCCTAGTTACTTCATTCATATCTGTATATAGTACTCCCTCCGTCCCGAATTAATTGACCCCTTGGCTCATTTCACACTTATTAAGAAAAATGTATAAATAAAGGGGAAAGTAGAATATATTGTATTGGGAATGATAAAAGTAGTATTATTTAGTGTTATATTGTATTGGGAATGATGAAAGTTGTATTGAAAATTGAAATGGGTCAATTATTTTGGGACACTTTTTTTTAGAAAATGGGTCAGTTATTCCGGGACGGAGGGAGTAGTTGCTAGTCAAATGTGATGGTGTTTTCGACTTTTTGGCTATGTAAATTTCTGCACTTCTCACATGATCTGTGTGTCTGATTCATGAAGTGGGGTAGTGTTACTTCGGGTAAAGGATTCTCTTACACACGTATCTTTTTTCTACTGATCACTCACTCTTTCCTTGTTCTTCGTACAAAAATTGTTCGTACAAATATAGCATTGAGCTGTTTAGACGAAATTAAGTTATATCCTTGAAAAAAAATTATATATGTTCCTTTCTTTTTCATTCTTTCCATGTTAGTGTCACATATTTTACTTTTGGAATCATTTTTCTTTTACTTTTTTAGTCACTTTCAGTGAAATTACCTACGTCAACGTACCTAAATTTTCATTTTTTTTTTATAATTTATTTATTTTTCTTAACAATATATATGTCCATTTCTATTTAATATAGATAGATATCGACTCAACCATATAAAAATTGGATAATTAAAGTGAAGTGATATAGACTGTACGGAAAAAGTAGAAATTGGAATTAATTATGGTAGGGTATAGCTAGCATGTGTAAAAATAAAAAAGATAAGTGAAAAAGTTAATAAAATGTGAAAAGTCAAAAACAGTGACGTATTTTGTTGATATTTACACAGGGTGCCATGCAGAAAGCGTGGTGGAATAAGATTCACCGTGAACGGATTCCGTTACTTTAACCTGGTGCTAATTAGCAACGTAGCAGGTGCTGGTGATATCGTGCGCGCCTACGTGAAAGGAACCCGAACCGAGTGGATGCCACTGAGCCGAAACTGGGGTCAGAACTGGCAATCCAACGCGGTTTTAGTGGGCCAGGCTTTGTCCTTTCGTGTTTCAAGCAGTGACCGTAGATCTTCAACATCTTGGAACATTGCTCCTCCTAGTTGGCAATTTGGTCAAACTTTCACTGGAAAGAATTTTAGGGTTTAA
Protein sequence

>MS.gene41164.t1

MVAKMLYIAFIIIISMYIVEARIPGVYSGGQWQDAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFEIKCANDKEWCHSGSPSIFITATNFCPPNFAQPSDNGGWCNPPRPHFDLAMPMFLKIAEYRAGIVPVAYRRVPCRKRGGIRFTVNGFRYFNLVLISNVAGAGDIVRAYVKGTRTEWMPLSRNWGQNWQSNAVLVGQALSFRVSSSDRRSSTSWNIAPPSWQFGQTFTGKNFRV