AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071182

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071182.t1 MTR_4g063975 98.450 258 4 0 1 258 1 258 0.0 524
MS.gene071182.t1 MTR_2g097030 86.822 258 34 0 1 258 3 260 1.38e-172 476
MS.gene071182.t1 MTR_5g079950 80.934 257 46 1 2 258 6 259 2.63e-162 450
MS.gene071182.t1 MTR_7g061720 82.171 258 42 1 5 258 1 258 2.56e-159 442
MS.gene071182.t1 MTR_5g041700 71.179 229 64 1 29 257 22 248 3.48e-123 350
MS.gene071182.t1 MTR_3g094060 65.323 248 82 2 11 256 8 253 1.17e-122 350
MS.gene071182.t1 MTR_3g094040 64.173 254 86 3 5 256 3 253 1.52e-121 347
MS.gene071182.t1 MTR_4g081950 64.822 253 82 3 2 253 34 280 3.06e-120 345
MS.gene071182.t1 MTR_5g075320 63.386 254 88 3 5 256 1 251 8.66e-119 340
MS.gene071182.t1 MTR_3g435430 69.778 225 66 1 32 256 25 247 1.87e-118 338
MS.gene071182.t1 MTR_3g466760 62.891 256 93 1 1 256 7 260 4.19e-117 336
MS.gene071182.t1 MTR_3g466790 63.035 257 91 3 1 256 1 254 2.72e-116 333
MS.gene071182.t1 MTR_7g011060 63.265 245 80 2 12 256 6 240 2.47e-114 328
MS.gene071182.t1 MTR_1g085460 60.744 242 87 4 16 254 18 254 2.35e-105 306
MS.gene071182.t1 MTR_6g012520 62.698 252 84 2 5 256 1 242 2.44e-105 305
MS.gene071182.t1 MTR_7g111010 56.917 253 101 4 2 251 1 248 3.75e-105 305
MS.gene071182.t1 MTR_1g026020 58.498 253 101 3 5 254 1 252 2.34e-103 301
MS.gene071182.t1 MTR_4g124340 59.471 227 89 2 30 255 59 283 3.68e-103 301
MS.gene071182.t1 MTR_7g110990 62.332 223 80 3 32 251 12 233 3.61e-102 297
MS.gene071182.t1 MTR_8g464350 54.656 247 107 2 5 251 7 248 1.59e-98 288
MS.gene071182.t1 MTR_7g013150 59.375 224 89 1 31 254 29 250 7.64e-98 286
MS.gene071182.t1 MTR_7g013140 57.658 222 92 1 32 253 28 247 3.04e-94 277
MS.gene071182.t1 MTR_8g058990 56.757 222 94 1 32 253 30 249 1.26e-93 276
MS.gene071182.t1 MTR_7g013120 53.333 240 107 3 16 254 15 250 2.35e-92 273
MS.gene071182.t1 MTR_4g102450 51.779 253 116 4 10 257 11 262 1.83e-91 271
MS.gene071182.t1 MTR_5g011210 50.397 252 122 3 8 257 13 263 1.05e-90 269
MS.gene071182.t1 MTR_0040s0140 46.875 256 135 1 1 256 2 256 6.35e-88 261
MS.gene071182.t1 MTR_5g076950 55.310 226 100 1 31 256 40 264 7.82e-88 262
MS.gene071182.t1 MTR_2g090255 48.261 230 119 0 27 256 23 252 6.59e-83 249
MS.gene071182.t1 MTR_3g064510 52.766 235 102 5 31 257 52 285 2.31e-80 243
MS.gene071182.t1 MTR_0008s0120 59.091 110 45 0 144 253 86 195 4.22e-42 142
MS.gene071182.t1 MTR_0008s0120 58.228 79 30 1 10 88 13 88 2.08e-23 94.4
MS.gene071182.t1 MTR_6g012500 42.857 175 84 4 98 256 106 280 1.57e-40 141
MS.gene071182.t1 MTR_2g090270 29.218 243 146 8 24 254 27 255 1.36e-24 99.0
MS.gene071182.t1 MTR_2g090265 28.195 266 162 10 4 254 2 253 9.87e-23 94.0
MS.gene071182.t1 MTR_0122s0020 31.356 236 136 10 31 254 40 261 1.65e-22 93.6
MS.gene071182.t1 MTR_4g028640 29.237 236 132 11 32 251 42 258 4.18e-18 81.6
MS.gene071182.t1 MTR_0118s0070 28.571 231 135 12 37 251 53 269 2.05e-16 77.0
MS.gene071182.t1 MTR_0809s0010 28.926 242 136 13 27 251 42 264 9.23e-16 75.1
MS.gene071182.t1 MTR_0122s0020 33.333 198 98 11 21 198 21 204 1.53e-14 70.9
MS.gene071182.t1 MTR_5g013440 25.991 227 151 9 35 254 32 248 1.15e-13 68.9
MS.gene071182.t1 MTR_4g099370 25.751 233 152 10 28 251 24 244 3.25e-13 67.8
MS.gene071182.t1 MTR_4g075790 28.000 225 127 9 43 251 32 237 3.67e-13 67.8
MS.gene071182.t1 MTR_1g085365 53.571 56 26 0 201 256 4 59 3.86e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071182.t1 AT2G39700 83.399 253 42 0 6 258 5 257 3.22e-162 450
MS.gene071182.t1 AT2G28950 83.529 255 41 1 5 258 3 257 8.27e-156 434
MS.gene071182.t1 AT3G55500 77.734 256 57 0 3 258 5 260 9.19e-153 426
MS.gene071182.t1 AT2G37640 78.968 252 50 1 10 258 11 262 5.00e-152 424
MS.gene071182.t1 AT5G02260 74.419 258 66 0 1 258 1 258 1.82e-151 422
MS.gene071182.t1 AT2G37640 81.905 210 38 0 49 258 28 237 6.22e-131 370
MS.gene071182.t1 AT1G69530 66.403 253 82 2 5 257 1 250 3.21e-123 351
MS.gene071182.t1 AT1G69530 66.403 253 82 2 5 257 1 250 3.21e-123 351
MS.gene071182.t1 AT1G69530 66.008 253 83 2 5 257 1 250 1.31e-121 348
MS.gene071182.t1 AT5G56320 67.769 242 74 2 16 257 15 252 6.35e-121 345
MS.gene071182.t1 AT5G56320 67.769 242 74 2 16 257 15 252 6.35e-121 345
MS.gene071182.t1 AT2G03090 66.403 253 78 3 5 257 8 253 1.64e-120 344
MS.gene071182.t1 AT5G56320 67.769 242 74 2 16 257 15 252 3.05e-120 345
MS.gene071182.t1 AT1G26770 66.403 253 81 2 5 257 1 249 8.21e-117 335
MS.gene071182.t1 AT1G26770 66.142 254 82 2 4 257 10 259 1.06e-116 335
MS.gene071182.t1 AT5G05290 64.000 250 86 3 8 256 9 255 2.97e-115 331
MS.gene071182.t1 AT2G40610 61.719 256 92 3 2 256 3 253 5.06e-115 330
MS.gene071182.t1 AT3G29030 65.929 226 68 2 32 257 38 254 3.48e-110 318
MS.gene071182.t1 AT1G69530 61.265 253 65 3 5 257 1 220 1.46e-107 310
MS.gene071182.t1 AT4G01630 61.475 244 86 5 11 251 11 249 3.57e-107 310
MS.gene071182.t1 AT1G69530 60.870 253 66 3 5 257 1 220 3.46e-106 308
MS.gene071182.t1 AT1G20190 61.086 221 83 2 34 252 28 247 3.24e-96 283
MS.gene071182.t1 AT5G39290 49.640 278 112 5 1 254 3 276 5.44e-94 278
MS.gene071182.t1 AT5G39270 57.848 223 90 2 32 254 58 276 8.60e-94 277
MS.gene071182.t1 AT1G62980 53.469 245 112 2 11 254 10 253 4.21e-92 272
MS.gene071182.t1 AT1G12560 51.172 256 118 5 4 257 12 262 7.52e-90 267
MS.gene071182.t1 AT5G39280 55.157 223 96 2 32 254 54 272 9.25e-90 267
MS.gene071182.t1 AT5G39300 54.709 223 97 2 32 254 55 273 6.17e-89 265
MS.gene071182.t1 AT5G39310 54.464 224 97 3 32 254 90 309 3.54e-86 259
MS.gene071182.t1 AT3G03220 53.509 228 105 1 31 258 39 265 4.68e-86 257
MS.gene071182.t1 AT4G38210 46.311 244 129 1 16 257 12 255 1.68e-80 243
MS.gene071182.t1 AT3G15370 47.368 247 119 6 12 254 8 247 3.28e-76 232
MS.gene071182.t1 AT5G39260 52.000 225 98 3 32 255 45 260 6.46e-76 231
MS.gene071182.t1 AT1G20190 57.447 188 77 2 67 252 2 188 5.29e-75 226
MS.gene071182.t1 AT3G15370 42.238 277 119 7 12 254 8 277 1.22e-69 216
MS.gene071182.t1 AT4G28250 30.705 241 138 11 28 254 32 257 1.07e-23 96.7
MS.gene071182.t1 AT2G20750 29.167 240 145 9 31 256 39 267 6.90e-22 92.0
MS.gene071182.t1 AT1G65680 30.126 239 138 11 28 251 43 267 4.86e-20 87.0
MS.gene071182.t1 AT4G28250 29.583 240 136 11 28 254 32 251 6.75e-20 86.3
MS.gene071182.t1 AT2G20750 32.386 176 97 7 31 195 39 203 3.95e-16 75.5
MS.gene071182.t1 AT4G28250 32.240 183 95 8 28 200 32 195 1.19e-15 73.9
MS.gene071182.t1 AT4G28250 32.584 178 91 8 28 195 32 190 8.22e-15 71.6
MS.gene071182.t1 AT3G60570 23.194 263 173 8 10 258 12 259 2.15e-13 68.6
MS.gene071182.t1 AT4G17030 24.757 206 140 6 9 209 4 199 1.95e-12 65.9
MS.gene071182.t1 AT3G45970 25.000 212 137 9 51 251 15 215 6.08e-12 63.9

Find 66 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCAATGTCGAGCATTT+TGG 0.206356 4.2:-40959996 None:intergenic
TCACTTGCTTGAGCAAAATT+AGG 0.238221 4.2:-40960583 None:intergenic
GCTCCTCCTAGTTGGCAATT+TGG 0.267981 4.2:+40962261 MS.gene071182:CDS
GACAAAGAGTGGTGTCATTC+TGG 0.278401 4.2:+40960520 MS.gene071182:CDS
GATGCTCATGCTACTTTCTA+TGG 0.312345 4.2:+40960088 MS.gene071182:CDS
TATGGTGGATCTGATGCTTC+TGG 0.339901 4.2:+40960106 MS.gene071182:CDS
GCCAGTTCTGACCCCAGTTT+CGG 0.341334 4.2:-40962155 None:intergenic
GGATGCCACTGAGCCGAAAC+TGG 0.348922 4.2:+40962142 MS.gene071182:CDS
TTTGCTCAAGCAAGTGATAA+TGG 0.368817 4.2:+40960589 MS.gene071182:CDS
CAATTTGGTCAAACTTTCAC+TGG 0.389947 4.2:+40962276 MS.gene071182:CDS
ACTAACCTGCGGTAAGCAAC+AGG 0.390868 4.2:-40960697 None:intergenic
ATTGTTGAAGCTAGAATTCC+AGG 0.394127 4.2:+40960040 MS.gene071182:CDS
CAGTGGCATCCACTCGGTTC+GGG 0.402414 4.2:-40962130 None:intergenic
TCAGTGGCATCCACTCGGTT+CGG 0.423453 4.2:-40962131 None:intergenic
ACCGTAGATCTTCAACATCT+TGG 0.445386 4.2:+40962232 MS.gene071182:CDS
GCCGAAACTGGGGTCAGAAC+TGG 0.448238 4.2:+40962154 MS.gene071182:CDS
CGGATTCCGTTACTTTAACC+TGG 0.456362 4.2:+40962050 MS.gene071182:CDS
GTTTGACCAAATTGCCAACT+AGG 0.460335 4.2:-40962267 None:intergenic
GGAACATTGCTCCTCCTAGT+TGG 0.491170 4.2:+40962253 MS.gene071182:CDS
GTAGCAGTAATGAAAATTGA+AGG 0.495390 4.2:-40960547 None:intergenic
TCTGATGCTTCTGGCACCAT+GGG 0.503667 4.2:+40960115 MS.gene071182:CDS
GGAATAAGATTCACCGTGAA+CGG 0.509029 4.2:+40962030 MS.gene071182:CDS
TTAGGAGGAGCATGTGGGTA+CGG 0.517583 4.2:+40960391 MS.gene071182:intron
ATTCCACCGCGCTTTCTGCA+TGG 0.520175 4.2:-40962012 None:intergenic
GTGTTTACTCTGGTGGTCAA+TGG 0.521019 4.2:+40960062 MS.gene071182:CDS
TTGTCCCTGTTGCTTACCGC+AGG 0.522475 4.2:+40960692 MS.gene071182:CDS
TCAGCAATCTTAAGAAACAT+AGG 0.524078 4.2:-40960655 None:intergenic
AATTTGTACAGTCAAGGTTA+CGG 0.526954 4.2:+40960415 MS.gene071182:CDS
ATTAGCAACGTAGCAGGTGC+TGG 0.528629 4.2:+40962078 MS.gene071182:CDS
ATCTGATGCTTCTGGCACCA+TGG 0.529188 4.2:+40960114 MS.gene071182:CDS
GTGCTAATTAGCAACGTAGC+AGG 0.532373 4.2:+40962072 MS.gene071182:CDS
CTACGTTGCTAATTAGCACC+AGG 0.535801 4.2:-40962068 None:intergenic
AGAATTCCAGGTGTTTACTC+TGG 0.536978 4.2:+40960052 MS.gene071182:CDS
AAAGTAACGGAATCCGTTCA+CGG 0.539938 4.2:-40962043 None:intergenic
TCCAAGATGTTGAAGATCTA+CGG 0.544252 4.2:-40962233 None:intergenic
GATGCCACTGAGCCGAAACT+GGG 0.544292 4.2:+40962143 MS.gene071182:CDS
ATCGTGCGCGCCTACGTGAA+AGG 0.554876 4.2:+40962105 MS.gene071182:CDS
TACGGAAATTTGTACAGTCA+AGG 0.556701 4.2:+40960409 MS.gene071182:CDS
AAACACGAAAGGACAAAGCC+TGG 0.564825 4.2:-40962200 None:intergenic
ATTCCAGGTGTTTACTCTGG+TGG 0.567430 4.2:+40960055 MS.gene071182:CDS
AAGATTGCTGAGTATCGTGC+TGG 0.570660 4.2:+40960667 MS.gene071182:CDS
TGACCACCAGAGTAAACACC+TGG 0.570964 4.2:-40960058 None:intergenic
AGCACTGCTTTGTTCAACAA+TGG 0.578791 4.2:+40960457 MS.gene071182:CDS
GGTTCGGGTTCCTTTCACGT+AGG 0.581009 4.2:-40962115 None:intergenic
TCGGCTCAGTGGCATCCACT+CGG 0.585195 4.2:-40962136 None:intergenic
CTTGCTTGAGCAAAATTAGG+AGG 0.594414 4.2:-40960580 None:intergenic
TTCAACAATGGTTTAAGCTG+TGG 0.599006 4.2:+40960469 MS.gene071182:CDS
TTAGCACCAGGTTAAAGTAA+CGG 0.608652 4.2:-40962056 None:intergenic
GGCATAGCAAGATCAAAATG+AGG 0.611060 4.2:-40960634 None:intergenic
GCTCATGCTACTTTCTATGG+TGG 0.618802 4.2:+40960091 MS.gene071182:CDS
TGACCCCAGTTTCGGCTCAG+TGG 0.625730 4.2:-40962147 None:intergenic
CTGGCCCACTAAAACCGCGT+TGG 0.630033 4.2:-40962181 None:intergenic
GCAAGATCAAAATGAGGACG+AGG 0.630560 4.2:-40960628 None:intergenic
GTCACTGCTTGAAACACGAA+AGG 0.633040 4.2:-40962211 None:intergenic
TGACCAAATTGCCAACTAGG+AGG 0.638092 4.2:-40962264 None:intergenic
AGATCAAAATGAGGACGAGG+AGG 0.649689 4.2:-40960625 None:intergenic
TGAAAGGAACCCGAACCGAG+TGG 0.653524 4.2:+40962121 MS.gene071182:CDS
AAGCAAGTGATAATGGTGGT+TGG 0.657192 4.2:+40960596 MS.gene071182:CDS
GTGCCATGCAGAAAGCGCGG+TGG 0.659131 4.2:+40962009 MS.gene071182:CDS
GATCAAAATGAGGACGAGGA+GGG 0.663864 4.2:-40960624 None:intergenic
ATGCCACTGAGCCGAAACTG+GGG 0.678002 4.2:+40962144 MS.gene071182:CDS
AGGGTGCCATGCAGAAAGCG+CGG 0.679047 4.2:+40962006 MS.gene071182:intron
AGTGTGCAAATGACAAAGAG+TGG 0.680419 4.2:+40960509 MS.gene071182:CDS
CTAACCTGCGGTAAGCAACA+GGG 0.690792 4.2:-40960696 None:intergenic
GCTCAAGCAAGTGATAATGG+TGG 0.723102 4.2:+40960592 MS.gene071182:CDS
TCAGAACTGGCAATCCAACG+CGG 0.772854 4.2:+40962167 MS.gene071182:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACTACTAAAAAAAATTA+AGG - chr4.2:40961193-40961212 None:intergenic 10.0%
!!! AAGTTTATTTTATTTTACTT+TGG + chr4.2:40960932-40960951 MS.gene071182:intron 10.0%
!!! ATTTTATCTTTAAGTTATTT+AGG + chr4.2:40960904-40960923 MS.gene071182:intron 10.0%
!!! TTAATTATCCAATTTTTATA+TGG - chr4.2:40961834-40961853 None:intergenic 10.0%
!!! ATATATTATTGACTTTTCTT+TGG + chr4.2:40961248-40961267 MS.gene071182:intron 15.0%
!!! ATGTTTTTTTAGGTTTAATT+AGG - chr4.2:40960329-40960348 None:intergenic 15.0%
!!! TTTATGTTAATTTTTTGTGT+TGG + chr4.2:40960363-40960382 MS.gene071182:intron 15.0%
!!! TTTTCTTTTTCATTAATTGA+GGG - chr4.2:40961221-40961240 None:intergenic 15.0%
!! AAAAGAAAGAAAGAAATTAG+AGG + chr4.2:40961115-40961134 MS.gene071182:intron 20.0%
!! AAATTGGAATTAATTATGGT+AGG + chr4.2:40961887-40961906 MS.gene071182:intron 20.0%
!! AATTGGAATTAATTATGGTA+GGG + chr4.2:40961888-40961907 MS.gene071182:intron 20.0%
!! GTAGAAATTGGAATTAATTA+TGG + chr4.2:40961883-40961902 MS.gene071182:intron 20.0%
!! TACATTACTTGAACAAAAAA+AGG - chr4.2:40960729-40960748 None:intergenic 20.0%
!! TATAGTTAAGAAATTAGTTG+TGG + chr4.2:40961282-40961301 MS.gene071182:intron 20.0%
!! TTAAGGTTAAATTATATTCC+TGG - chr4.2:40961176-40961195 None:intergenic 20.0%
!!! AAATAATAGTATAAAGTACC+AGG + chr4.2:40961155-40961174 MS.gene071182:intron 20.0%
!!! ATTTTGTTGATATTTACACA+GGG + chr4.2:40961987-40962006 MS.gene071182:intron 20.0%
!!! CTTTTCTTTTTCATTAATTG+AGG - chr4.2:40961222-40961241 None:intergenic 20.0%
!!! GTAACATTTCATGTTTTTTT+AGG - chr4.2:40960339-40960358 None:intergenic 20.0%
!!! TATTTTGTTGATATTTACAC+AGG + chr4.2:40961986-40962005 MS.gene071182:intron 20.0%
!!! TTTTTGTTCAAGTAATGTAT+GGG + chr4.2:40960730-40960749 MS.gene071182:intron 20.0%
!!! TTTTTTGTTCAAGTAATGTA+TGG + chr4.2:40960729-40960748 MS.gene071182:intron 20.0%
! AAAATGAAGTAGTAAAAGAG+TGG - chr4.2:40960828-40960847 None:intergenic 25.0%
! AATCACTTTGACATATAGTT+AGG + chr4.2:40960204-40960223 MS.gene071182:intron 25.0%
! AGAAATGAATGAAGTAACTA+GGG - chr4.2:40961363-40961382 None:intergenic 25.0%
! ATCACTTTGACATATAGTTA+GGG + chr4.2:40960205-40960224 MS.gene071182:intron 25.0%
! ATGGAAAGAATGAAAAAGAA+AGG - chr4.2:40961651-40961670 None:intergenic 25.0%
! CTACTCAACCATATAAAAAT+TGG + chr4.2:40961823-40961842 MS.gene071182:intron 25.0%
! GATACAAGTGGTAAATATTT+AGG + chr4.2:40960969-40960988 MS.gene071182:intron 25.0%
! TAAAATATGAGACACTAACA+TGG - chr4.2:40961670-40961689 None:intergenic 25.0%
! TATCCACATGTTTGTATTTA+CGG - chr4.2:40960280-40960299 None:intergenic 25.0%
!! AGTGTCTCATATTTTACTTT+TGG + chr4.2:40961674-40961693 MS.gene071182:intron 25.0%
!! TTTCACTGGAAAGAATTTTA+GGG + chr4.2:40962290-40962309 MS.gene071182:CDS 25.0%
!! TTTTGTTCAAGTAATGTATG+GGG + chr4.2:40960731-40960750 MS.gene071182:intron 25.0%
!!! TTTTTTGTGTTGGTGTTTTT+AGG + chr4.2:40960373-40960392 MS.gene071182:intron 25.0%
AATTTGTACAGTCAAGGTTA+CGG + chr4.2:40960415-40960434 MS.gene071182:CDS 30.0%
AGTTAAGAAATTAGTTGTGG+TGG + chr4.2:40961285-40961304 MS.gene071182:intron 30.0%
ATAGACGCATTATTATATGC+AGG - chr4.2:40960780-40960799 None:intergenic 30.0%
CAGAAATGAATGAAGTAACT+AGG - chr4.2:40961364-40961383 None:intergenic 30.0%
GTAGCAGTAATGAAAATTGA+AGG - chr4.2:40960550-40960569 None:intergenic 30.0%
TAGACGCATTATTATATGCA+GGG - chr4.2:40960779-40960798 None:intergenic 30.0%
TCAGCAATCTTAAGAAACAT+AGG - chr4.2:40960658-40960677 None:intergenic 30.0%
TCTACGGAAAAAGTAGAAAT+TGG + chr4.2:40961871-40961890 MS.gene071182:intron 30.0%
TCTTAAGTTTGTGATACAAG+TGG + chr4.2:40960957-40960976 MS.gene071182:intron 30.0%
! CAATTTTTGTACGAAGAACA+AGG - chr4.2:40961558-40961577 None:intergenic 30.0%
! CTTTCACTGGAAAGAATTTT+AGG + chr4.2:40962289-40962308 MS.gene071182:CDS 30.0%
AAAGGAAAGTAACTAACCTG+CGG - chr4.2:40960711-40960730 None:intergenic 35.0%
AAATTTGTGTACGTTGACGT+AGG - chr4.2:40961743-40961762 None:intergenic 35.0%
AGAAAATACGAGGGCAAAAA+GGG - chr4.2:40960162-40960181 None:intergenic 35.0%
ATTATTATATGCAGGGCTTG+GGG - chr4.2:40960772-40960791 None:intergenic 35.0%
ATTGTTGAAGCTAGAATTCC+AGG + chr4.2:40960040-40960059 MS.gene071182:CDS 35.0%
CAATTTGGTCAAACTTTCAC+TGG + chr4.2:40962276-40962295 MS.gene071182:CDS 35.0%
CATTATTATATGCAGGGCTT+GGG - chr4.2:40960773-40960792 None:intergenic 35.0%
GACATAATCTCGACGTATTA+GGG - chr4.2:40960250-40960269 None:intergenic 35.0%
GTGAAGTGATATAGACTCTA+CGG + chr4.2:40961855-40961874 MS.gene071182:intron 35.0%
TACGGAAATTTGTACAGTCA+AGG + chr4.2:40960409-40960428 MS.gene071182:CDS 35.0%
TCACCGTAAATACAAACATG+TGG + chr4.2:40960274-40960293 MS.gene071182:intron 35.0%
TCACTTGCTTGAGCAAAATT+AGG - chr4.2:40960586-40960605 None:intergenic 35.0%
TCCAAGATGTTGAAGATCTA+CGG - chr4.2:40962236-40962255 None:intergenic 35.0%
TGACATAATCTCGACGTATT+AGG - chr4.2:40960251-40960270 None:intergenic 35.0%
TTAGCACCAGGTTAAAGTAA+CGG - chr4.2:40962059-40962078 None:intergenic 35.0%
TTCAACAATGGTTTAAGCTG+TGG + chr4.2:40960469-40960488 MS.gene071182:CDS 35.0%
TTCATTAACTGTGATGAGAC+TGG - chr4.2:40961314-40961333 None:intergenic 35.0%
TTCCAACTGAATTAAGCTCT+TGG + chr4.2:40960799-40960818 MS.gene071182:intron 35.0%
TTCCAAGAGCTTAATTCAGT+TGG - chr4.2:40960804-40960823 None:intergenic 35.0%
TTTGCTCAAGCAAGTGATAA+TGG + chr4.2:40960589-40960608 MS.gene071182:CDS 35.0%
! AAAGCAATGTCGAGCATTTT+GGG - chr4.2:40959998-40960017 None:intergenic 35.0%
!! GGTAATTTGTTACATACCCA+TGG - chr4.2:40960134-40960153 None:intergenic 35.0%
!! TGATGGTGTTTTCGACTTTT+TGG + chr4.2:40961407-40961426 MS.gene071182:intron 35.0%
!!! TTTGTGTTGGTGTTTTTAGG+AGG + chr4.2:40960376-40960395 MS.gene071182:intron 35.0%
AAGTAATGTATGGGGTTTCC+GGG + chr4.2:40960739-40960758 MS.gene071182:intron 40.0%
ACCGTAGATCTTCAACATCT+TGG + chr4.2:40962232-40962251 MS.gene071182:CDS 40.0%
AGAATTCCAGGTGTTTACTC+TGG + chr4.2:40960052-40960071 MS.gene071182:CDS 40.0%
AGTGTGCAAATGACAAAGAG+TGG + chr4.2:40960509-40960528 MS.gene071182:CDS 40.0%
CAAGTAATGTATGGGGTTTC+CGG + chr4.2:40960738-40960757 MS.gene071182:intron 40.0%
CAGAAAATACGAGGGCAAAA+AGG - chr4.2:40960163-40960182 None:intergenic 40.0%
CCTCAATCCAGAAAATACGA+GGG - chr4.2:40960171-40960190 None:intergenic 40.0%
CTTGCTTGAGCAAAATTAGG+AGG - chr4.2:40960583-40960602 None:intergenic 40.0%
GCATTATTATATGCAGGGCT+TGG - chr4.2:40960774-40960793 None:intergenic 40.0%
GGAATAAGATTCACCGTGAA+CGG + chr4.2:40962030-40962049 MS.gene071182:CDS 40.0%
GGCATAGCAAGATCAAAATG+AGG - chr4.2:40960637-40960656 None:intergenic 40.0%
GTGTGTCTGATTCATGAAGT+GGG + chr4.2:40961462-40961481 MS.gene071182:intron 40.0%
GTTGCTCTAGTCAAATGTGA+TGG + chr4.2:40961390-40961409 MS.gene071182:intron 40.0%
GTTTGACCAAATTGCCAACT+AGG - chr4.2:40962270-40962289 None:intergenic 40.0%
TGTGTCTGATTCATGAAGTG+GGG + chr4.2:40961463-40961482 MS.gene071182:intron 40.0%
TGTGTGTCTGATTCATGAAG+TGG + chr4.2:40961461-40961480 MS.gene071182:intron 40.0%
! AAAGTAACGGAATCCGTTCA+CGG - chr4.2:40962046-40962065 None:intergenic 40.0%
! AGCACTGCTTTGTTCAACAA+TGG + chr4.2:40960457-40960476 MS.gene071182:CDS 40.0%
! CCTCGTATTTTCTGGATTGA+GGG + chr4.2:40960169-40960188 MS.gene071182:intron 40.0%
! CTTTTTGCCCTCGTATTTTC+TGG + chr4.2:40960161-40960180 MS.gene071182:intron 40.0%
! GAAAGCAATGTCGAGCATTT+TGG - chr4.2:40959999-40960018 None:intergenic 40.0%
!! AAGCAAGTGATAATGGTGGT+TGG + chr4.2:40960596-40960615 MS.gene071182:CDS 40.0%
!! GATGCTCATGCTACTTTCTA+TGG + chr4.2:40960088-40960107 MS.gene071182:CDS 40.0%
!!! TGTTTTTAGGAGGAGCATGT+GGG + chr4.2:40960386-40960405 MS.gene071182:intron 40.0%
AAACACGAAAGGACAAAGCC+TGG - chr4.2:40962203-40962222 None:intergenic 45.0%
ACGAGGGCAAAAAGGGAAAT+GGG - chr4.2:40960155-40960174 None:intergenic 45.0%
AGATCAAAATGAGGACGAGG+AGG - chr4.2:40960628-40960647 None:intergenic 45.0%
AGTAATGTATGGGGTTTCCG+GGG + chr4.2:40960740-40960759 MS.gene071182:intron 45.0%
ATTCCAGGTGTTTACTCTGG+TGG + chr4.2:40960055-40960074 MS.gene071182:CDS 45.0%
CCCTCAATCCAGAAAATACG+AGG - chr4.2:40960172-40960191 None:intergenic 45.0%
CTACGTTGCTAATTAGCACC+AGG - chr4.2:40962071-40962090 None:intergenic 45.0%
GATCAAAATGAGGACGAGGA+GGG - chr4.2:40960627-40960646 None:intergenic 45.0%
GCAAGATCAAAATGAGGACG+AGG - chr4.2:40960631-40960650 None:intergenic 45.0%
GTCACTGCTTGAAACACGAA+AGG - chr4.2:40962214-40962233 None:intergenic 45.0%
GTGCTAATTAGCAACGTAGC+AGG + chr4.2:40962072-40962091 MS.gene071182:CDS 45.0%
GTGTTTACTCTGGTGGTCAA+TGG + chr4.2:40960062-40960081 MS.gene071182:CDS 45.0%
TACGAGGGCAAAAAGGGAAA+TGG - chr4.2:40960156-40960175 None:intergenic 45.0%
TGACCAAATTGCCAACTAGG+AGG - chr4.2:40962267-40962286 None:intergenic 45.0%
! AAGATTGCTGAGTATCGTGC+TGG + chr4.2:40960667-40960686 MS.gene071182:CDS 45.0%
! CCCTCGTATTTTCTGGATTG+AGG + chr4.2:40960168-40960187 MS.gene071182:intron 45.0%
! CGGATTCCGTTACTTTAACC+TGG + chr4.2:40962050-40962069 MS.gene071182:CDS 45.0%
!! AAGTGGGGTAGTGTTACTTC+GGG + chr4.2:40961478-40961497 MS.gene071182:intron 45.0%
!! CAATCCAACGCGGTTTTAGT+GGG + chr4.2:40962177-40962196 MS.gene071182:CDS 45.0%
!! GAAGTGGGGTAGTGTTACTT+CGG + chr4.2:40961477-40961496 MS.gene071182:intron 45.0%
!! GACAAAGAGTGGTGTCATTC+TGG + chr4.2:40960520-40960539 MS.gene071182:CDS 45.0%
!! GCTCAAGCAAGTGATAATGG+TGG + chr4.2:40960592-40960611 MS.gene071182:CDS 45.0%
!! GCTCATGCTACTTTCTATGG+TGG + chr4.2:40960091-40960110 MS.gene071182:CDS 45.0%
!! GGTAGTGTTACTTCGGGTAA+AGG + chr4.2:40961484-40961503 MS.gene071182:intron 45.0%
!! TATGGTGGATCTGATGCTTC+TGG + chr4.2:40960106-40960125 MS.gene071182:CDS 45.0%
!!! GTGTTTTTAGGAGGAGCATG+TGG + chr4.2:40960385-40960404 MS.gene071182:intron 45.0%
!!! ATTATTTTTAGATTTTATTT+TGG - chr4.2:40961142-40961161 None:intergenic 5.0%
!!! TCATTTTTTTATTATTTTTT+TGG + chr4.2:40960841-40960860 MS.gene071182:intron 5.0%
ACTAACCTGCGGTAAGCAAC+AGG - chr4.2:40960700-40960719 None:intergenic 50.0%
ATCTGATGCTTCTGGCACCA+TGG + chr4.2:40960114-40960133 MS.gene071182:CDS 50.0%
ATTAGCAACGTAGCAGGTGC+TGG + chr4.2:40962078-40962097 MS.gene071182:CDS 50.0%
CTAACCTGCGGTAAGCAACA+GGG - chr4.2:40960699-40960718 None:intergenic 50.0%
GCTCCTCCTAGTTGGCAATT+TGG + chr4.2:40962261-40962280 MS.gene071182:CDS 50.0%
GGAACATTGCTCCTCCTAGT+TGG + chr4.2:40962253-40962272 MS.gene071182:CDS 50.0%
TCAGAACTGGCAATCCAACG+CGG + chr4.2:40962167-40962186 MS.gene071182:CDS 50.0%
TCTGATGCTTCTGGCACCAT+GGG + chr4.2:40960115-40960134 MS.gene071182:CDS 50.0%
TGACCACCAGAGTAAACACC+TGG - chr4.2:40960061-40960080 None:intergenic 50.0%
! TTAGGAGGAGCATGTGGGTA+CGG + chr4.2:40960391-40960410 MS.gene071182:intron 50.0%
!! GCAATCCAACGCGGTTTTAG+TGG + chr4.2:40962176-40962195 MS.gene071182:CDS 50.0%
ATGCCACTGAGCCGAAACTG+GGG + chr4.2:40962144-40962163 MS.gene071182:CDS 55.0%
ATTCCACCGCGCTTTCTGCA+TGG - chr4.2:40962015-40962034 None:intergenic 55.0%
GATGCCACTGAGCCGAAACT+GGG + chr4.2:40962143-40962162 MS.gene071182:CDS 55.0%
TCAGTGGCATCCACTCGGTT+CGG - chr4.2:40962134-40962153 None:intergenic 55.0%
TGAAAGGAACCCGAACCGAG+TGG + chr4.2:40962121-40962140 MS.gene071182:CDS 55.0%
! GCCAGTTCTGACCCCAGTTT+CGG - chr4.2:40962158-40962177 None:intergenic 55.0%
! GGTTCGGGTTCCTTTCACGT+AGG - chr4.2:40962118-40962137 None:intergenic 55.0%
! TTGTCCCTGTTGCTTACCGC+AGG + chr4.2:40960692-40960711 MS.gene071182:CDS 55.0%
ATCGTGCGCGCCTACGTGAA+AGG + chr4.2:40962105-40962124 MS.gene071182:CDS 60.0%
CAGTGGCATCCACTCGGTTC+GGG - chr4.2:40962133-40962152 None:intergenic 60.0%
CTGGCCCACTAAAACCGCGT+TGG - chr4.2:40962184-40962203 None:intergenic 60.0%
GCCGAAACTGGGGTCAGAAC+TGG + chr4.2:40962154-40962173 MS.gene071182:CDS 60.0%
GGATGCCACTGAGCCGAAAC+TGG + chr4.2:40962142-40962161 MS.gene071182:CDS 60.0%
TCGGCTCAGTGGCATCCACT+CGG - chr4.2:40962139-40962158 None:intergenic 60.0%
TGACCCCAGTTTCGGCTCAG+TGG - chr4.2:40962150-40962169 None:intergenic 60.0%
! AGGGTGCCATGCAGAAAGCG+CGG + chr4.2:40962006-40962025 MS.gene071182:intron 60.0%
!! CAACGCGGTTTTAGTGGGCC+AGG + chr4.2:40962182-40962201 MS.gene071182:CDS 60.0%
! GTGCCATGCAGAAAGCGCGG+TGG + chr4.2:40962009-40962028 MS.gene071182:CDS 65.0%
AGGGCTTGGGGTTCGAGCCC+CGG - chr4.2:40960760-40960779 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 40959989 40962317 40959989 ID=MS.gene071182
chr4.2 mRNA 40959989 40962317 40959989 ID=MS.gene071182.t1;Parent=MS.gene071182
chr4.2 exon 40959989 40960136 40959989 ID=MS.gene071182.t1.exon1;Parent=MS.gene071182.t1
chr4.2 CDS 40959989 40960136 40959989 ID=cds.MS.gene071182.t1;Parent=MS.gene071182.t1
chr4.2 exon 40960395 40960713 40960395 ID=MS.gene071182.t1.exon2;Parent=MS.gene071182.t1
chr4.2 CDS 40960395 40960713 40960395 ID=cds.MS.gene071182.t1;Parent=MS.gene071182.t1
chr4.2 exon 40962008 40962317 40962008 ID=MS.gene071182.t1.exon3;Parent=MS.gene071182.t1
chr4.2 CDS 40962008 40962317 40962008 ID=cds.MS.gene071182.t1;Parent=MS.gene071182.t1
Gene Sequence

>MS.gene071182

ATGGTGCCCAAAATGCTCGACATTGCTTTCATAATTATCACTTCAATGTACATTGTTGAAGCTAGAATTCCAGGTGTTTACTCTGGTGGTCAATGGCAAGATGCTCATGCTACTTTCTATGGTGGATCTGATGCTTCTGGCACCATGGGTATGTAACAAATTACCCATTTCCCTTTTTGCCCTCGTATTTTCTGGATTGAGGGTGAGTTTGTCAAAATCACTTTGACATATAGTTAGGGTTGCATGTATAGCTTAAAACCCTAATACGTCGAGATTATGTCAAATTCACCGTAAATACAAACATGTGGATAAAATACATACAAATAATTACAATGTACCTAATTAAACCTAAAAAAACATGAAATGTTACTATGTTTATGTTAATTTTTTGTGTTGGTGTTTTTAGGAGGAGCATGTGGGTACGGAAATTTGTACAGTCAAGGTTACGGAGTGAACACAGCAGCATTAAGCACTGCTTTGTTCAACAATGGTTTAAGCTGTGGTGCTTGTTTTGAGATAAAGTGTGCAAATGACAAAGAGTGGTGTCATTCTGGAAGTCCTTCAATTTTCATTACTGCTACTAACTTTTGTCCTCCTAATTTTGCTCAAGCAAGTGATAATGGTGGTTGGTGTAACCCTCCTCGTCCTCATTTTGATCTTGCTATGCCTATGTTTCTTAAGATTGCTGAGTATCGTGCTGGTATTGTCCCTGTTGCTTACCGCAGGTTAGTTACTTTCCTTTTTTTGTTCAAGTAATGTATGGGGTTTCCGGGGCTCGAACCCCAAGCCCTGCATATAATAATGCGTCTATTCCAACTGAATTAAGCTCTTGGAAACCACTCTTTTACTACTTCATTTTTTTATTATTTTTTTGGTATAAACTTCATTTTTAATTTTAGACGAATTAAACTTTACATTTTATCTTTAAGTTATTTAGGTCTCTAAGTTTATTTTATTTTACTTTGGTGTCTTAAGTTTGTGATACAAGTGGTAAATATTTAGGTCTCTTAATAAAGAAAAACATTTTGTTTCAATTTTTAAATAAAGTCTATAATGCTGTTAGAACAAAAACAATTTATAGAGACAAAGTGTATAGTACTTGTCTGTCACTTAAATAACTAAAATGAAAAGAAAGAAAGAAATTAGAGGACCAAAATAAAATCTAAAAATAATAGTATAAAGTACCAGGAATATAATTTAACCTTAATTTTTTTTAGTAGTATAGTACTCCCTCAATTAATGAAAAAGAAAAGTAGTTTATATATTATTGACTTTTCTTTGGAAAATGATGTTTATAGTTAAGAAATTAGTTGTGGTGGAGTCCAGTCTCATCACAGTTAATGAAAAAAGAAAAGTAGCTTATATTATTGACCCTAGTTACTTCATTCATTTCTGTATGTAGTTGCTCTAGTCAAATGTGATGGTGTTTTCGACTTTTTGGCTATGTAAATTTCTGCACTTCTCACATGATCTGTGTGTCTGATTCATGAAGTGGGGTAGTGTTACTTCGGGTAAAGGATTCTCTTACACACGTAGTATCTTTTTTCTACTGATCACTCACTCTTTCCTTGTTCTTCGTACAAAAATTGTTCGTACAAATATAGCATTGAGCTGTTTAGACGAAATTAAGTTATATACTTGAAAAAAATTATATATGTTCCTTTCTTTTTCATTCTTTCCATGTTAGTGTCTCATATTTTACTTTTGGAATCATTTTTATTTTACTTTTTTAGTCACTTTCAGTGAAATTACCTACGTCAACGTACACAAATTTTATTTTTTATAATTTATTTATTTTTCTTAACTACGTCATTTCTATTTAATGTAGATATATCTACTCAACCATATAAAAATTGGATAATTAAAGTGAAGTGATATAGACTCTACGGAAAAAGTAGAAATTGGAATTAATTATGGTAGGGTATAGCTAGCATGTGTAAAAATAAAAAAGATAAGTGAAAAAGTTAATAAAATGTGAAAAGTCAAAAACAGTGACGTATTTTGTTGATATTTACACAGGGTGCCATGCAGAAAGCGCGGTGGAATAAGATTCACCGTGAACGGATTCCGTTACTTTAACCTGGTGCTAATTAGCAACGTAGCAGGTGCTGGTGATATCGTGCGCGCCTACGTGAAAGGAACCCGAACCGAGTGGATGCCACTGAGCCGAAACTGGGGTCAGAACTGGCAATCCAACGCGGTTTTAGTGGGCCAGGCTTTGTCCTTTCGTGTTTCAAGCAGTGACCGTAGATCTTCAACATCTTGGAACATTGCTCCTCCTAGTTGGCAATTTGGTCAAACTTTCACTGGAAAGAATTTTAGGGTTTAA
Protein sequence

>MS.gene071182.t1

MVPKMLDIAFIIITSMYIVEARIPGVYSGGQWQDAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFEIKCANDKEWCHSGSPSIFITATNFCPPNFAQASDNGGWCNPPRPHFDLAMPMFLKIAEYRAGIVPVAYRRVPCRKRGGIRFTVNGFRYFNLVLISNVAGAGDIVRAYVKGTRTEWMPLSRNWGQNWQSNAVLVGQALSFRVSSSDRRSSTSWNIAPPSWQFGQTFTGKNFRV