Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028493.t1 | RHN62652.1 | 99.2 | 765 | 6 | 0 | 1 | 765 | 1 | 765 | 0.00E+00 | 1467.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028493.t1 | Q8S944 | 71.7 | 759 | 196 | 5 | 9 | 757 | 31 | 780 | 1.6e-297 | 1023.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028493.t1 | A0A396IAM3 | 99.2 | 765 | 6 | 0 | 1 | 765 | 1 | 765 | 0.0e+00 | 1467.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene028493 | MS.gene39347 | PPI |
| MS.gene059335 | MS.gene028493 | PPI |
| MS.gene028493 | MS.gene047680 | PPI |
| MS.gene028493 | MS.gene002248 | PPI |
| MS.gene028493 | MS.gene010879 | PPI |
| MS.gene028493 | MS.gene049382 | PPI |
| MS.gene028493 | MS.gene059331 | PPI |
| MS.gene06506 | MS.gene028493 | PPI |
| MS.gene06504 | MS.gene028493 | PPI |
| MS.gene010407 | MS.gene028493 | PPI |
| MS.gene01221 | MS.gene028493 | PPI |
| MS.gene050237 | MS.gene028493 | PPI |
| MS.gene028493 | MS.gene01221 | PPI |
| MS.gene028493 | MS.gene02205 | PPI |
| MS.gene056130 | MS.gene028493 | PPI |
| MS.gene028493 | MS.gene041137 | PPI |
| MS.gene028493 | MS.gene068015 | PPI |
| MS.gene90230 | MS.gene028493 | PPI |
| MS.gene028493 | MS.gene71381 | PPI |
| MS.gene028493 | MS.gene013407 | PPI |
| MS.gene028493 | MS.gene053588 | PPI |
| MS.gene022695 | MS.gene028493 | PPI |
| MS.gene028493 | MS.gene26060 | PPI |
| MS.gene028493 | MS.gene013409 | PPI |
| MS.gene059333 | MS.gene028493 | PPI |
| MS.gene39665 | MS.gene028493 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028493.t1 | MTR_4g093750 | 98.936 | 752 | 6 | 1 | 1 | 750 | 1 | 752 | 0.0 | 1521 |
| MS.gene028493.t1 | MTR_4g093750 | 98.955 | 670 | 5 | 1 | 1 | 670 | 1 | 668 | 0.0 | 1347 |
| MS.gene028493.t1 | MTR_2g009690 | 83.026 | 760 | 120 | 3 | 11 | 763 | 20 | 777 | 0.0 | 1281 |
| MS.gene028493.t1 | MTR_5g009150 | 38.632 | 497 | 285 | 4 | 15 | 499 | 4 | 492 | 2.57e-104 | 333 |
| MS.gene028493.t1 | MTR_4g098920 | 40.323 | 496 | 275 | 6 | 14 | 497 | 3 | 489 | 3.92e-100 | 322 |
| MS.gene028493.t1 | MTR_8g099550 | 38.540 | 493 | 282 | 5 | 15 | 495 | 4 | 487 | 1.32e-99 | 320 |
| MS.gene028493.t1 | MTR_8g027675 | 37.652 | 494 | 288 | 5 | 15 | 497 | 4 | 488 | 8.07e-97 | 313 |
| MS.gene028493.t1 | MTR_8g009510 | 38.431 | 497 | 282 | 5 | 14 | 495 | 6 | 493 | 2.42e-94 | 307 |
| MS.gene028493.t1 | MTR_8g105580 | 38.134 | 493 | 285 | 6 | 13 | 495 | 5 | 487 | 5.46e-94 | 305 |
| MS.gene028493.t1 | MTR_8g105580 | 38.134 | 493 | 285 | 6 | 13 | 495 | 5 | 487 | 9.95e-94 | 305 |
| MS.gene028493.t1 | MTR_7g451010 | 39.525 | 463 | 267 | 4 | 37 | 495 | 40 | 493 | 3.13e-92 | 301 |
| MS.gene028493.t1 | MTR_1g032550 | 36.714 | 493 | 293 | 5 | 13 | 495 | 5 | 488 | 4.29e-90 | 295 |
| MS.gene028493.t1 | MTR_8g009510 | 34.518 | 394 | 245 | 4 | 106 | 495 | 2 | 386 | 8.53e-59 | 209 |
| MS.gene028493.t1 | MTR_8g087750 | 37.654 | 324 | 182 | 7 | 35 | 352 | 69 | 378 | 1.19e-45 | 174 |
| MS.gene028493.t1 | MTR_8g087770 | 34.739 | 403 | 234 | 14 | 35 | 425 | 66 | 451 | 1.87e-43 | 167 |
| MS.gene028493.t1 | MTR_4g030140 | 22.495 | 489 | 317 | 16 | 40 | 499 | 42 | 497 | 1.10e-16 | 85.1 |
| MS.gene028493.t1 | MTR_8g463270 | 30.288 | 208 | 128 | 4 | 20 | 217 | 31 | 231 | 5.41e-15 | 79.7 |
| MS.gene028493.t1 | MTR_4g094300 | 30.052 | 193 | 127 | 5 | 32 | 217 | 35 | 226 | 1.57e-13 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene028493.t1 | AT4G33650 | 72.605 | 741 | 192 | 4 | 7 | 737 | 29 | 768 | 0.0 | 1085 |
| MS.gene028493.t1 | AT4G33650 | 72.237 | 742 | 194 | 4 | 7 | 737 | 29 | 769 | 0.0 | 1081 |
| MS.gene028493.t1 | AT2G14120 | 71.583 | 739 | 195 | 6 | 10 | 736 | 16 | 751 | 0.0 | 1059 |
| MS.gene028493.t1 | AT2G14120 | 71.448 | 739 | 196 | 6 | 10 | 736 | 16 | 751 | 0.0 | 1057 |
| MS.gene028493.t1 | AT2G14120 | 68.880 | 768 | 195 | 7 | 10 | 736 | 16 | 780 | 0.0 | 1043 |
| MS.gene028493.t1 | AT2G14120 | 71.508 | 723 | 191 | 6 | 10 | 720 | 16 | 735 | 0.0 | 1036 |
| MS.gene028493.t1 | AT5G42080 | 38.742 | 493 | 281 | 5 | 15 | 495 | 4 | 487 | 3.49e-100 | 322 |
| MS.gene028493.t1 | AT5G42080 | 38.742 | 493 | 281 | 5 | 15 | 495 | 4 | 487 | 3.49e-100 | 322 |
| MS.gene028493.t1 | AT3G61760 | 38.755 | 498 | 278 | 10 | 14 | 496 | 3 | 488 | 2.84e-98 | 317 |
| MS.gene028493.t1 | AT3G61760 | 38.755 | 498 | 278 | 10 | 14 | 496 | 3 | 488 | 3.04e-98 | 316 |
| MS.gene028493.t1 | AT5G42080 | 38.540 | 493 | 276 | 6 | 15 | 495 | 4 | 481 | 1.37e-97 | 315 |
| MS.gene028493.t1 | AT5G42080 | 41.589 | 428 | 231 | 4 | 15 | 432 | 4 | 422 | 4.34e-97 | 308 |
| MS.gene028493.t1 | AT3G61760 | 40.260 | 462 | 251 | 9 | 14 | 462 | 3 | 452 | 1.53e-96 | 308 |
| MS.gene028493.t1 | AT3G60190 | 37.405 | 524 | 302 | 7 | 14 | 522 | 6 | 518 | 1.40e-94 | 308 |
| MS.gene028493.t1 | AT2G44590 | 37.449 | 494 | 289 | 4 | 14 | 495 | 3 | 488 | 3.95e-93 | 303 |
| MS.gene028493.t1 | AT1G14830 | 37.602 | 492 | 288 | 5 | 14 | 495 | 6 | 488 | 2.36e-92 | 301 |
| MS.gene028493.t1 | AT2G44590 | 35.223 | 494 | 283 | 5 | 14 | 495 | 3 | 471 | 3.33e-80 | 268 |
| MS.gene028493.t1 | AT2G44590 | 35.223 | 494 | 283 | 5 | 14 | 495 | 3 | 471 | 3.59e-80 | 268 |
| MS.gene028493.t1 | AT1G60500 | 31.311 | 511 | 316 | 16 | 9 | 503 | 33 | 524 | 8.08e-49 | 183 |
| MS.gene028493.t1 | AT1G60530 | 39.493 | 276 | 144 | 7 | 9 | 275 | 30 | 291 | 2.01e-43 | 159 |
| MS.gene028493.t1 | AT1G10290 | 22.822 | 482 | 322 | 15 | 40 | 499 | 41 | 494 | 4.54e-18 | 89.7 |
| MS.gene028493.t1 | AT1G59610 | 22.614 | 482 | 323 | 14 | 40 | 499 | 41 | 494 | 9.55e-18 | 88.6 |
| MS.gene028493.t1 | AT1G53140 | 28.311 | 219 | 145 | 4 | 20 | 228 | 47 | 263 | 4.90e-12 | 70.1 |
| MS.gene028493.t1 | AT3G19720 | 29.082 | 196 | 125 | 7 | 32 | 217 | 43 | 234 | 8.15e-11 | 66.2 |
| MS.gene028493.t1 | AT3G19720 | 29.082 | 196 | 125 | 7 | 32 | 217 | 43 | 234 | 8.88e-11 | 65.9 |
Find 191 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACTTCCACCAATAAAATT+TGG | 0.140968 | 4.1:-20173392 | None:intergenic |
| CCTGAATTTCAGCACGGATT+TGG | 0.258507 | 4.1:-20168483 | None:intergenic |
| CTCTAAACTCCTCACAAATA+TGG | 0.260098 | 4.1:-20170445 | None:intergenic |
| CTGAATTTCAGCACGGATTT+GGG | 0.261460 | 4.1:-20168482 | None:intergenic |
| TTAGGCAAGCCAGTTGTTTC+AGG | 0.269599 | 4.1:-20177595 | None:intergenic |
| CCAAATCCGTGCTGAAATTC+AGG | 0.289470 | 4.1:+20168483 | MS.gene028493:CDS |
| TCATGTCTTGCAGGCTGGAC+AGG | 0.292960 | 4.1:+20170127 | MS.gene028493:intron |
| ATGAAGAAACTCAGCGGATT+CGG | 0.317533 | 4.1:-20168431 | None:intergenic |
| TAAGGCGATCATGCACTTCT+TGG | 0.319960 | 4.1:+20174496 | MS.gene028493:CDS |
| AAACGCATGGATGAAGTTAT+TGG | 0.325769 | 4.1:+20171677 | MS.gene028493:CDS |
| CGATGCTCGGAATCTGTTAC+TGG | 0.328569 | 4.1:+20169140 | MS.gene028493:CDS |
| CAAGTCTTCTACAGTTTCTA+AGG | 0.329354 | 4.1:+20173442 | MS.gene028493:CDS |
| CTCATTCTTTCCCTCTACTA+TGG | 0.332851 | 4.1:-20170367 | None:intergenic |
| TACTTCCACCAATAAAATTT+GGG | 0.334413 | 4.1:-20173391 | None:intergenic |
| CATATTTGTGAGGAGTTTAG+AGG | 0.340009 | 4.1:+20170446 | MS.gene028493:CDS |
| ACCATCGGGATCAGCATTTC+CGG | 0.368627 | 4.1:-20168864 | None:intergenic |
| ATGTATTCTACTAGCAGTTC+AGG | 0.370412 | 4.1:+20177643 | MS.gene028493:CDS |
| GTTGCAGGTCATAGAACTAT+AGG | 0.371796 | 4.1:+20168977 | MS.gene028493:intron |
| TGAAGAAACTCAGCGGATTC+GGG | 0.376300 | 4.1:-20168430 | None:intergenic |
| TCTTCATCGCAAATCTATAT+AGG | 0.379851 | 4.1:+20175110 | MS.gene028493:CDS |
| AAACTCAGCGGATTCGGGTT+TGG | 0.389522 | 4.1:-20168425 | None:intergenic |
| TACACCGGCTAATTCAGATT+TGG | 0.392589 | 4.1:+20168817 | MS.gene028493:CDS |
| AACCACTGGTGGATCTAGTT+GGG | 0.393292 | 4.1:+20174120 | MS.gene028493:CDS |
| TTCAACTTCTTTGCCAATTG+AGG | 0.398038 | 4.1:-20169643 | None:intergenic |
| TCTGATGGCCTCAAGATAGT+AGG | 0.401234 | 4.1:-20174374 | None:intergenic |
| TCTGACAAACCGGAGTATAA+AGG | 0.407487 | 4.1:+20169464 | MS.gene028493:CDS |
| TCATTGTCTCTGAGGGTTCA+AGG | 0.410651 | 4.1:-20171718 | None:intergenic |
| GAGGCAGATATCTCGGCTAT+TGG | 0.417224 | 4.1:+20171467 | MS.gene028493:CDS |
| ATGTCTACATCTGAGCTATC+AGG | 0.419688 | 4.1:+20170393 | MS.gene028493:CDS |
| CACATCAAGGCTGTGCTACC+AGG | 0.427513 | 4.1:+20169899 | MS.gene028493:CDS |
| TGGATTGTAGTTGGATATCA+TGG | 0.427886 | 4.1:+20169107 | MS.gene028493:intron |
| GATGCTGTGGAGTCTGATAA+AGG | 0.428618 | 4.1:+20173688 | MS.gene028493:CDS |
| CACAGTTCGCCTGAAACAAC+TGG | 0.430116 | 4.1:+20177586 | MS.gene028493:CDS |
| CGCTAGACTTGCCGCTACTC+TGG | 0.431697 | 4.1:-20168306 | None:intergenic |
| ATGGATAAAGTATTACATTC+AGG | 0.432828 | 4.1:+20173923 | MS.gene028493:CDS |
| TGTGCGTGCTTCATCAGATA+TGG | 0.435906 | 4.1:+20173904 | MS.gene028493:CDS |
| TCAGATCAGCCATCGTTGTA+TGG | 0.438010 | 4.1:+20171622 | MS.gene028493:intron |
| TTTGTTGACTAGTGAGATTA+CGG | 0.438850 | 4.1:-20168212 | None:intergenic |
| ATCCAAGTCTTCAATGTGCC+AGG | 0.439754 | 4.1:+20171490 | MS.gene028493:CDS |
| GATCACCCACATCATAGAAA+TGG | 0.442767 | 4.1:+20171739 | MS.gene028493:CDS |
| TCCTAGCGGGGCGCCGTCGT+TGG | 0.445295 | 4.1:-20168185 | None:intergenic |
| GTGCTATTCTAGATATATAG+TGG | 0.449866 | 4.1:+20169598 | MS.gene028493:intron |
| TAGTTGTGGCGTTCCTCAAT+TGG | 0.451776 | 4.1:+20169630 | MS.gene028493:CDS |
| CTGATGGCCTCAAGATAGTA+GGG | 0.454854 | 4.1:-20174373 | None:intergenic |
| CTCCCTCAGGTTGCCGTCGT+CGG | 0.457022 | 4.1:+20168280 | MS.gene028493:CDS |
| GAACCACTGGTGGATCTAGT+TGG | 0.459664 | 4.1:+20174119 | MS.gene028493:CDS |
| ACCGCCATACAGAATGCAAC+TGG | 0.461119 | 4.1:+20171275 | MS.gene028493:CDS |
| ATCAGGATATTCTGACAAAC+CGG | 0.462757 | 4.1:+20169454 | MS.gene028493:intron |
| CATGTCTTGCAGGCTGGACA+GGG | 0.466689 | 4.1:+20170128 | MS.gene028493:intron |
| AAGAAGTGCATGATCGCCTT+AGG | 0.473310 | 4.1:-20174494 | None:intergenic |
| GATGGAAGCACACTTTGAAC+TGG | 0.475219 | 4.1:-20174220 | None:intergenic |
| TTGAAGCTGAAACGGTTGAG+TGG | 0.476160 | 4.1:+20177560 | MS.gene028493:CDS |
| CCGGAGTATAAAGGATGCTC+TGG | 0.477256 | 4.1:+20169473 | MS.gene028493:CDS |
| GAATTCTGATGCTCTTCAAA+TGG | 0.479089 | 4.1:+20168841 | MS.gene028493:CDS |
| TCTTATTCTTGCTGTTACAC+CGG | 0.479476 | 4.1:+20168802 | MS.gene028493:CDS |
| ATGATCAGCCATTCCTCCAT+TGG | 0.480288 | 4.1:-20173761 | None:intergenic |
| CCCCTCCGACTTGGTTATGT+CGG | 0.488295 | 4.1:+20169174 | MS.gene028493:CDS |
| GCTGAGACTGATAGGGAAGC+TGG | 0.491118 | 4.1:+20168602 | MS.gene028493:CDS |
| AGCGTAATGTCAAGAACATT+CGG | 0.491446 | 4.1:-20168674 | None:intergenic |
| GTCTCTGAACTTCTCTCTGA+TGG | 0.501227 | 4.1:-20174389 | None:intergenic |
| GCTGAGTTTCTTCATTTACC+TGG | 0.502907 | 4.1:+20168439 | MS.gene028493:CDS |
| AGGTCCAGTTGCATTCTGTA+TGG | 0.505132 | 4.1:-20171279 | None:intergenic |
| GAGGGAGGTCGATGGTGGAT+TGG | 0.505265 | 4.1:-20168264 | None:intergenic |
| GAGATATCTGCCTCCGTACA+AGG | 0.505354 | 4.1:-20171458 | None:intergenic |
| TGTTGAGGATCTTATTCCTA+AGG | 0.508752 | 4.1:+20174478 | MS.gene028493:CDS |
| CCCTTTCTTGAGAAAACGCA+TGG | 0.509114 | 4.1:+20171664 | MS.gene028493:CDS |
| CTCCGTACAAGGACTTCAAA+CGG | 0.509243 | 4.1:-20171447 | None:intergenic |
| TATATATGACGAGTTAATGA+AGG | 0.509634 | 4.1:+20171515 | MS.gene028493:CDS |
| AACCTGGCACATTGAAGACT+TGG | 0.509646 | 4.1:-20171492 | None:intergenic |
| TTCCGTTTGAAGTCCTTGTA+CGG | 0.510952 | 4.1:+20171445 | MS.gene028493:CDS |
| AGATTACGGTGGATCCTAGC+GGG | 0.512805 | 4.1:-20168198 | None:intergenic |
| CTTAGAAACTGTAGAAGACT+TGG | 0.514125 | 4.1:-20173441 | None:intergenic |
| CTCAAATGAATTGTAGAGGA+TGG | 0.514624 | 4.1:-20174238 | None:intergenic |
| TTGTACGGAGGCAGATATCT+CGG | 0.515018 | 4.1:+20171460 | MS.gene028493:CDS |
| TGTTCTTGACATTACGCTTG+TGG | 0.517699 | 4.1:+20168679 | MS.gene028493:CDS |
| TGAAGCTGAAACGGTTGAGT+GGG | 0.519060 | 4.1:+20177561 | MS.gene028493:CDS |
| TCCAACGACGGCGCCCCGCT+AGG | 0.520684 | 4.1:+20168184 | MS.gene028493:CDS |
| CTTATGCGTGCCTTCAGCCC+TGG | 0.521367 | 4.1:-20169917 | None:intergenic |
| TCTGATGCTCTTCAAATGGC+CGG | 0.522566 | 4.1:+20168845 | MS.gene028493:CDS |
| AAAGTTATTCCCCTCCGACT+TGG | 0.523941 | 4.1:+20169165 | MS.gene028493:CDS |
| TTCTACAGTTTCTAAGGTGA+AGG | 0.527882 | 4.1:+20173448 | MS.gene028493:CDS |
| GCGGATTCGGGTTTGGAAGG+TGG | 0.529941 | 4.1:-20168418 | None:intergenic |
| ATACCTCCATTTCTATGATG+TGG | 0.530780 | 4.1:-20171745 | None:intergenic |
| ATCCACCATCGACCTCCCTC+AGG | 0.531578 | 4.1:+20168267 | MS.gene028493:CDS |
| GCTACTCTGGCTGCCGACGA+CGG | 0.532120 | 4.1:-20168293 | None:intergenic |
| GCAAAGGAACATGCAAGCTA+TGG | 0.532385 | 4.1:+20169959 | MS.gene028493:CDS |
| ACTACCTCTTGAAGCTGAAA+CGG | 0.534191 | 4.1:+20177552 | MS.gene028493:CDS |
| GTAGAATACATAGAAGAAGT+TGG | 0.534563 | 4.1:-20177631 | None:intergenic |
| CGGTGTTGTAAATCGTAGCC+AGG | 0.534919 | 4.1:+20169194 | MS.gene028493:CDS |
| GGAACAAACAATGCTGCTCT+AGG | 0.536557 | 4.1:-20171299 | None:intergenic |
| ACGGGAACCTTGGTAATACC+AGG | 0.537492 | 4.1:-20168707 | None:intergenic |
| TTCCACCAATAAAATTTGGG+TGG | 0.538529 | 4.1:-20173388 | None:intergenic |
| TTGATCACCAACGGGAACCT+TGG | 0.539530 | 4.1:-20168717 | None:intergenic |
| TCTGAAGGTTGATCACCAAC+GGG | 0.540101 | 4.1:-20168725 | None:intergenic |
| GGCTGTGCTACCAGGGCTGA+AGG | 0.540827 | 4.1:+20169907 | MS.gene028493:CDS |
| ACTGGCTTGCCTAAAATTCG+CGG | 0.540843 | 4.1:+20177604 | MS.gene028493:CDS |
| TCAGCGGATTCGGGTTTGGA+AGG | 0.543433 | 4.1:-20168421 | None:intergenic |
| TTTGTAGGCTGAGACTGATA+GGG | 0.547959 | 4.1:+20168595 | MS.gene028493:intron |
| TAAAAGCTTGTTGATAAGCT+CGG | 0.551504 | 4.1:-20175302 | None:intergenic |
| ATCTGAAGGTTGATCACCAA+CGG | 0.552721 | 4.1:-20168726 | None:intergenic |
| TGTATCCCCTCAGGAACCAC+TGG | 0.558758 | 4.1:+20174106 | MS.gene028493:intron |
| CTGCCGACGACGGCAACCTG+AGG | 0.559115 | 4.1:-20168283 | None:intergenic |
| CCCTAACCAAACTATGGCGG+CGG | 0.561738 | 4.1:+20168159 | None:intergenic |
| TCCACCAATAAAATTTGGGT+GGG | 0.563887 | 4.1:-20173387 | None:intergenic |
| TTCACTTGTCAATCTTGCAA+AGG | 0.564530 | 4.1:+20169943 | MS.gene028493:CDS |
| CCAGAGCATCCTTTATACTC+CGG | 0.564711 | 4.1:-20169473 | None:intergenic |
| GATTCTTGCACAACACATCA+AGG | 0.565933 | 4.1:+20169886 | MS.gene028493:intron |
| TGGAGAAATCACGGAGTCCA+AGG | 0.574229 | 4.1:+20169979 | MS.gene028493:CDS |
| ACAGCAAGAATAAGACAAGT+AGG | 0.575319 | 4.1:-20168794 | None:intergenic |
| TGTGCAGATATCGTTACCAC+GGG | 0.577032 | 4.1:-20168362 | None:intergenic |
| TGGACAGAGGTACCGATGCT+CGG | 0.577331 | 4.1:+20169127 | MS.gene028493:CDS |
| CACGGATTTGGGAGAAATCG+TGG | 0.577873 | 4.1:-20168471 | None:intergenic |
| CACGGCCGACGAGAGCTTCG+AGG | 0.578474 | 4.1:-20168330 | None:intergenic |
| AGATAACCGTGTGTCTGTGA+AGG | 0.581518 | 4.1:+20174168 | MS.gene028493:CDS |
| TTCGCCAAATCTGAATTAGC+CGG | 0.582042 | 4.1:-20168821 | None:intergenic |
| ATTACGCTTGTGGATCTCCC+TGG | 0.582531 | 4.1:+20168689 | MS.gene028493:CDS |
| AGCATTTACCTCCATAGTAG+AGG | 0.582545 | 4.1:+20170356 | MS.gene028493:intron |
| TGGTGGATTGGCTTCCGACA+CGG | 0.583448 | 4.1:-20168252 | None:intergenic |
| TGGGTGATCATTGTCTCTGA+GGG | 0.583520 | 4.1:-20171725 | None:intergenic |
| TACCTCCATTTCTATGATGT+GGG | 0.584511 | 4.1:-20171744 | None:intergenic |
| ATCCCCTCAGGAACCACTGG+TGG | 0.586660 | 4.1:+20174109 | MS.gene028493:intron |
| CGTTTGAAGTCCTTGTACGG+AGG | 0.587076 | 4.1:+20171448 | MS.gene028493:CDS |
| CAAGACATCTTCAGCCGTGT+CGG | 0.591438 | 4.1:+20168238 | MS.gene028493:CDS |
| TGCCGACGACGGCAACCTGA+GGG | 0.591564 | 4.1:-20168282 | None:intergenic |
| ACCACTGGTGGATCTAGTTG+GGG | 0.591737 | 4.1:+20174121 | MS.gene028493:CDS |
| CATAGTAGAGGGAAAGAATG+AGG | 0.594573 | 4.1:+20170368 | MS.gene028493:CDS |
| ATTCTTTCCCTCTACTATGG+AGG | 0.596542 | 4.1:-20170364 | None:intergenic |
| CTGAGCTATCAGGTGGAGCA+CGG | 0.598034 | 4.1:+20170403 | MS.gene028493:CDS |
| AGGGAAGCTGGAGGGAACAA+AGG | 0.599600 | 4.1:+20168614 | MS.gene028493:CDS |
| GTGGGTGATCATTGTCTCTG+AGG | 0.603242 | 4.1:-20171726 | None:intergenic |
| GGATCAGCTTCTGAAAGAAG+CGG | 0.604449 | 4.1:+20173709 | MS.gene028493:CDS |
| CTCTACAATTCATTTGAGAG+AGG | 0.605023 | 4.1:+20174243 | MS.gene028493:CDS |
| CAGTAACAGATTCCGAGCAT+CGG | 0.605265 | 4.1:-20169139 | None:intergenic |
| GGTATTACCAAGGTTCCCGT+TGG | 0.605870 | 4.1:+20168710 | MS.gene028493:CDS |
| CACCCACATCATAGAAATGG+AGG | 0.606305 | 4.1:+20171742 | MS.gene028493:CDS |
| ATTCTCGATTCGATATCTGA+AGG | 0.606620 | 4.1:-20168740 | None:intergenic |
| GCATTTACCTCCATAGTAGA+GGG | 0.607406 | 4.1:+20170357 | MS.gene028493:intron |
| GGATCTCCCTGGTATTACCA+AGG | 0.609003 | 4.1:+20168700 | MS.gene028493:CDS |
| CTCTCTCAAATGAATTGTAG+AGG | 0.611486 | 4.1:-20174242 | None:intergenic |
| TGCAAGCTATGGAGAAATCA+CGG | 0.611972 | 4.1:+20169970 | MS.gene028493:CDS |
| CTTGCAAGACATGCCAATGG+AGG | 0.614495 | 4.1:+20173748 | MS.gene028493:CDS |
| GATGCTCGGAATCTGTTACT+GGG | 0.618289 | 4.1:+20169141 | MS.gene028493:CDS |
| CACCGACATAACCAAGTCGG+AGG | 0.623102 | 4.1:-20169176 | None:intergenic |
| ACATCAAGGCTGTGCTACCA+GGG | 0.623492 | 4.1:+20169900 | MS.gene028493:CDS |
| AAGACATGCCAATGGAGGAA+TGG | 0.625106 | 4.1:+20173753 | MS.gene028493:CDS |
| GGCGGTACGAATATCATCAT+CGG | 0.625270 | 4.1:-20171258 | None:intergenic |
| AACCTGAGGGAGGTCGATGG+TGG | 0.626380 | 4.1:-20168269 | None:intergenic |
| GTCGTCGGCAGCCAGAGTAG+CGG | 0.628211 | 4.1:+20168295 | MS.gene028493:CDS |
| ATTCTTTCAAAATACTCCGA+CGG | 0.628519 | 4.1:+20170167 | MS.gene028493:CDS |
| AGCGTCCTCGAAGCTCTCGT+CGG | 0.629417 | 4.1:+20168325 | MS.gene028493:CDS |
| TCCAGTTGCATTCTGTATGG+CGG | 0.635952 | 4.1:-20171276 | None:intergenic |
| GGCAACCTGAGGGAGGTCGA+TGG | 0.638647 | 4.1:-20168272 | None:intergenic |
| ATGGCGGCGGTGTCCAACGA+CGG | 0.639282 | 4.1:+20168172 | MS.gene028493:CDS |
| TCAACCGTTTCAGCTTCAAG+AGG | 0.640027 | 4.1:-20177556 | None:intergenic |
| ATTCTTGCAAGACATGCCAA+TGG | 0.641227 | 4.1:+20173745 | MS.gene028493:CDS |
| GTGTGCAGATATCGTTACCA+CGG | 0.646207 | 4.1:-20168363 | None:intergenic |
| TGTGAACGAGTTGAAGAACA+AGG | 0.647322 | 4.1:-20168393 | None:intergenic |
| AACTATAGGTGTCATAACCA+AGG | 0.647581 | 4.1:+20168991 | MS.gene028493:CDS |
| ACCGACATAACCAAGTCGGA+GGG | 0.652428 | 4.1:-20169175 | None:intergenic |
| TAACTCGTCATATATAAACC+TGG | 0.654748 | 4.1:-20171508 | None:intergenic |
| AGTGGTCTAGCTGATAGTTG+TGG | 0.655528 | 4.1:+20169616 | MS.gene028493:CDS |
| TAATTTCCAATAGGATGCTG+TGG | 0.655752 | 4.1:+20173675 | MS.gene028493:intron |
| TGATGGCCTCAAGATAGTAG+GGG | 0.656712 | 4.1:-20174372 | None:intergenic |
| TGAACGAGTTGAAGAACAAG+GGG | 0.657953 | 4.1:-20168391 | None:intergenic |
| GTTGACTAGTGAGATTACGG+TGG | 0.659709 | 4.1:-20168209 | None:intergenic |
| TATTCTCCTTCACAGACACA+CGG | 0.662699 | 4.1:-20174174 | None:intergenic |
| GAGACTGATAGGGAAGCTGG+AGG | 0.664102 | 4.1:+20168605 | MS.gene028493:CDS |
| AAGGAACCTGAATTTCAGCA+CGG | 0.664519 | 4.1:-20168489 | None:intergenic |
| AAATCGTGGAAAGTACGACC+AGG | 0.665742 | 4.1:-20168457 | None:intergenic |
| GATGGCCTCAAGATAGTAGG+GGG | 0.667216 | 4.1:-20174371 | None:intergenic |
| CGACGACGGCAACCTGAGGG+AGG | 0.670636 | 4.1:-20168279 | None:intergenic |
| GAAGCTGAAACGGTTGAGTG+GGG | 0.671223 | 4.1:+20177562 | MS.gene028493:CDS |
| TCTACATCTGAGCTATCAGG+TGG | 0.673530 | 4.1:+20170396 | MS.gene028493:CDS |
| TCATCGGTCAAGTCTTCACA+AGG | 0.675461 | 4.1:-20171242 | None:intergenic |
| GCCGGAAATGCTGATCCCGA+TGG | 0.676099 | 4.1:+20168863 | MS.gene028493:CDS |
| ATTGGCAAAGAAGTTGAACA+AGG | 0.682461 | 4.1:+20169648 | MS.gene028493:CDS |
| CAACACCGACATAACCAAGT+CGG | 0.684240 | 4.1:-20169179 | None:intergenic |
| AGACTGATAGGGAAGCTGGA+GGG | 0.684734 | 4.1:+20168606 | MS.gene028493:CDS |
| GTGAACGAGTTGAAGAACAA+GGG | 0.684986 | 4.1:-20168392 | None:intergenic |
| CGGGAACCTTGGTAATACCA+GGG | 0.698106 | 4.1:-20168706 | None:intergenic |
| TAGTTGGATATCATGGACAG+AGG | 0.713407 | 4.1:+20169114 | MS.gene028493:intron |
| CCGACATAACCAAGTCGGAG+GGG | 0.714844 | 4.1:-20169174 | None:intergenic |
| GAGATTACGGTGGATCCTAG+CGG | 0.715884 | 4.1:-20168199 | None:intergenic |
| GTTCAGTCACCATACAACGA+TGG | 0.718083 | 4.1:-20171631 | None:intergenic |
| CCACTGGTGGATCTAGTTGG+GGG | 0.719711 | 4.1:+20174122 | MS.gene028493:CDS |
| TGCAGATATCGTTACCACGG+GGG | 0.724538 | 4.1:-20168360 | None:intergenic |
| GATTACGGTGGATCCTAGCG+GGG | 0.732843 | 4.1:-20168197 | None:intergenic |
| TGTTGTAAATCGTAGCCAGG+AGG | 0.732912 | 4.1:+20169197 | MS.gene028493:CDS |
| AGGTAAATGAAGAAACTCAG+CGG | 0.753833 | 4.1:-20168437 | None:intergenic |
| ACTTCCTTACAGGATGACTG+CGG | 0.758275 | 4.1:-20169515 | None:intergenic |
| GTGCAGATATCGTTACCACG+GGG | 0.769026 | 4.1:-20168361 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 20168172 | 20177678 | 20168172 | ID=MS.gene028493 |
| chr4.1 | mRNA | 20168172 | 20177678 | 20168172 | ID=MS.gene028493.t1;Parent=MS.gene028493 |
| chr4.1 | exon | 20168172 | 20168504 | 20168172 | ID=MS.gene028493.t1.exon1;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20168172 | 20168504 | 20168172 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20168602 | 20168884 | 20168602 | ID=MS.gene028493.t1.exon2;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20168602 | 20168884 | 20168602 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20168984 | 20169012 | 20168984 | ID=MS.gene028493.t1.exon3;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20168984 | 20169012 | 20168984 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20169117 | 20169218 | 20169117 | ID=MS.gene028493.t1.exon4;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20169117 | 20169218 | 20169117 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20169459 | 20169527 | 20169459 | ID=MS.gene028493.t1.exon5;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20169459 | 20169527 | 20169459 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20169610 | 20169669 | 20169610 | ID=MS.gene028493.t1.exon6;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20169610 | 20169669 | 20169610 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20169887 | 20170000 | 20169887 | ID=MS.gene028493.t1.exon7;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20169887 | 20170000 | 20169887 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20170140 | 20170188 | 20170140 | ID=MS.gene028493.t1.exon8;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20170140 | 20170188 | 20170140 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20170358 | 20170467 | 20170358 | ID=MS.gene028493.t1.exon9;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20170358 | 20170467 | 20170358 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20171233 | 20171325 | 20171233 | ID=MS.gene028493.t1.exon10;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20171233 | 20171325 | 20171233 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20171444 | 20171536 | 20171444 | ID=MS.gene028493.t1.exon11;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20171444 | 20171536 | 20171444 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20171626 | 20171763 | 20171626 | ID=MS.gene028493.t1.exon12;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20171626 | 20171763 | 20171626 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20173368 | 20173469 | 20173368 | ID=MS.gene028493.t1.exon13;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20173368 | 20173469 | 20173368 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20173688 | 20173783 | 20173688 | ID=MS.gene028493.t1.exon14;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20173688 | 20173783 | 20173688 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20173902 | 20173944 | 20173902 | ID=MS.gene028493.t1.exon15;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20173902 | 20173944 | 20173902 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20174119 | 20174264 | 20174119 | ID=MS.gene028493.t1.exon16;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20174119 | 20174264 | 20174119 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20174371 | 20174517 | 20174371 | ID=MS.gene028493.t1.exon17;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20174371 | 20174517 | 20174371 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20175079 | 20175131 | 20175079 | ID=MS.gene028493.t1.exon18;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20175079 | 20175131 | 20175079 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20175227 | 20175326 | 20175227 | ID=MS.gene028493.t1.exon19;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20175227 | 20175326 | 20175227 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| chr4.1 | exon | 20177541 | 20177678 | 20177541 | ID=MS.gene028493.t1.exon20;Parent=MS.gene028493.t1 |
| chr4.1 | CDS | 20177541 | 20177678 | 20177541 | ID=cds.MS.gene028493.t1;Parent=MS.gene028493.t1 |
| Gene Sequence |
| Protein sequence |
