AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39347

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39347.t1 MTR_8g009510 98.430 637 10 0 1 637 1 637 0.0 1297
MS.gene39347.t1 MTR_8g009510 98.302 530 9 0 108 637 1 530 0.0 1078
MS.gene39347.t1 MTR_7g451010 84.458 637 79 1 1 637 1 617 0.0 1078
MS.gene39347.t1 MTR_8g105580 76.063 635 132 3 1 635 1 615 0.0 1004
MS.gene39347.t1 MTR_1g032550 72.913 635 151 2 1 635 1 614 0.0 972
MS.gene39347.t1 MTR_4g098920 72.152 632 149 2 4 635 1 605 0.0 970
MS.gene39347.t1 MTR_8g105580 76.975 595 117 3 1 595 1 575 0.0 949
MS.gene39347.t1 MTR_8g099550 65.823 632 192 2 4 635 1 608 0.0 897
MS.gene39347.t1 MTR_5g009150 65.142 634 198 2 4 637 1 611 0.0 888
MS.gene39347.t1 MTR_8g027675 65.665 632 192 3 4 635 1 607 0.0 879
MS.gene39347.t1 MTR_2g009690 38.306 496 282 5 7 493 24 504 4.05e-97 316
MS.gene39347.t1 MTR_4g093750 38.431 497 282 5 6 493 14 495 4.80e-96 310
MS.gene39347.t1 MTR_4g093750 38.353 498 283 5 6 493 14 497 3.31e-95 309
MS.gene39347.t1 MTR_4g134680 30.695 518 322 11 1 501 1 498 4.13e-68 236
MS.gene39347.t1 MTR_4g134680 30.695 518 322 11 1 501 1 498 1.44e-67 236
MS.gene39347.t1 MTR_4g030140 30.948 517 325 9 1 501 1 501 4.28e-67 236
MS.gene39347.t1 MTR_4g134680 30.695 518 322 11 1 501 1 498 5.15e-67 236
MS.gene39347.t1 MTR_4g134680 38.806 201 123 0 301 501 1 201 2.16e-40 156
MS.gene39347.t1 MTR_8g087750 34.494 316 183 9 40 346 71 371 2.59e-31 130
MS.gene39347.t1 MTR_8g087770 34.906 318 179 10 40 346 68 368 5.30e-31 129
MS.gene39347.t1 MTR_8g463270 33.161 193 110 4 40 220 46 231 9.81e-19 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39347.t1 AT3G60190 78.336 637 125 2 1 637 1 624 0.0 1057
MS.gene39347.t1 AT1G14830 75.276 635 135 2 1 635 1 613 0.0 999
MS.gene39347.t1 AT2G44590 71.924 634 156 3 4 637 1 612 0.0 958
MS.gene39347.t1 AT2G44590 69.243 634 156 4 4 637 1 595 0.0 915
MS.gene39347.t1 AT2G44590 69.134 635 156 5 4 637 1 596 0.0 910
MS.gene39347.t1 AT5G42080 65.665 632 193 2 4 635 1 608 0.0 889
MS.gene39347.t1 AT5G42080 65.665 632 193 2 4 635 1 608 0.0 889
MS.gene39347.t1 AT3G61760 64.929 633 196 4 4 635 1 608 0.0 878
MS.gene39347.t1 AT5G42080 65.032 632 191 3 4 635 1 602 0.0 873
MS.gene39347.t1 AT3G61760 65.936 593 176 4 4 595 1 568 0.0 833
MS.gene39347.t1 AT3G61760 71.652 448 122 3 4 450 1 444 0.0 690
MS.gene39347.t1 AT5G42080 72.917 432 114 1 4 435 1 429 0.0 681
MS.gene39347.t1 AT2G14120 38.569 503 279 8 6 493 20 507 6.80e-98 316
MS.gene39347.t1 AT2G14120 38.569 503 279 8 6 493 20 507 2.70e-97 315
MS.gene39347.t1 AT2G14120 38.569 503 279 8 6 493 20 507 3.03e-97 315
MS.gene39347.t1 AT2G14120 36.654 532 278 9 6 493 20 536 3.06e-93 305
MS.gene39347.t1 AT4G33650 37.052 502 287 7 6 493 36 522 3.00e-89 295
MS.gene39347.t1 AT4G33650 37.052 502 287 7 6 493 36 522 3.04e-89 295
MS.gene39347.t1 AT1G10290 32.625 518 312 12 1 501 1 498 1.42e-67 238
MS.gene39347.t1 AT1G59610 32.563 519 311 14 1 501 1 498 5.04e-67 236
MS.gene39347.t1 AT1G60530 42.857 203 108 4 40 237 62 261 5.72e-37 140
MS.gene39347.t1 AT1G60500 34.528 307 188 7 40 344 65 360 4.81e-36 144
MS.gene39347.t1 AT1G53140 33.161 193 110 4 40 220 62 247 2.24e-19 93.2

Find 177 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAGGAAGATTACCGATTT+TGG 0.210097 8.2:-81587421 MS.gene39347:intron
TGTTCAAGATATTGAAAATA+TGG 0.234209 8.2:-81584994 MS.gene39347:CDS
AACTATTCGGCTTGTGGATA+TGG 0.268455 8.2:-81582171 MS.gene39347:CDS
ATGAAATTGATTCGGTTTCT+TGG 0.284411 8.2:-81581025 MS.gene39347:CDS
CAGTTTCGTCTTGAATTTCC+TGG 0.285024 8.2:+81586801 None:intergenic
TGGCCACTTAGCCAGTAAAA+TGG 0.287222 8.2:-81583627 MS.gene39347:CDS
CCTAACTTTGATTGATTTGC+CGG 0.293875 8.2:-81585167 MS.gene39347:CDS
TTCAGGGAAATCTTCGGTTT+TGG 0.304850 8.2:-81588236 MS.gene39347:CDS
AACTATTTCTACACACAAAT+AGG 0.315141 8.2:-81581861 MS.gene39347:CDS
GACTACTACTTTACCAAAAT+CGG 0.316139 8.2:+81587408 None:intergenic
CTGATGCTATCAAAATTTCC+AGG 0.329530 8.2:-81584036 MS.gene39347:CDS
TGGAGATGAGAACAGAGTTC+AGG 0.339919 8.2:-81588253 MS.gene39347:intron
TCTGGATTACCCCGATTAGA+TGG 0.350431 8.2:+81582065 None:intergenic
GAATAAATGCTAAGATGAAT+TGG 0.360365 8.2:+81586740 None:intergenic
AACCTACTAATCCATCTAAT+CGG 0.361526 8.2:-81582076 MS.gene39347:CDS
AGCACTTGGAGTCGGTAATT+CGG 0.363577 8.2:-81583502 MS.gene39347:CDS
GCACTTGGAGTCGGTAATTC+GGG 0.377924 8.2:-81583501 MS.gene39347:CDS
AGTTGTAGCTGCAGCTAATT+CGG 0.378066 8.2:+81582232 None:intergenic
CGAGTTTAGTACAAGCTTGT+TGG 0.379634 8.2:+81588433 None:intergenic
CTTCTTCGTGATGACGACTA+TGG 0.390068 8.2:-81588484 None:intergenic
AGTCTCCGGTAACCTTGCTC+TGG 0.390633 8.2:+81582568 None:intergenic
TGAAATTGATTCGGTTTCTT+GGG 0.392405 8.2:-81581024 MS.gene39347:CDS
GATGGATTAGTAGGTTGATC+TGG 0.393618 8.2:+81582047 None:intergenic
GGGACTCTCAGTTACTTTAG+AGG 0.394944 8.2:-81582538 MS.gene39347:CDS
GACGAAACTGATAGAATTAC+GGG 0.396420 8.2:-81586788 MS.gene39347:CDS
ATAGCCAGTATGATACAATT+AGG 0.396892 8.2:+81584088 None:intergenic
TTTGAAACCAGTCCTGACTA+TGG 0.400319 8.2:-81583647 MS.gene39347:CDS
TGACAATAAACCACAGCCTT+TGG 0.401522 8.2:+81581912 None:intergenic
GCAGCCTAATTGTATCATAC+TGG 0.404081 8.2:-81584092 MS.gene39347:intron
CCATTTCTTGAGGAAGTCTT+CGG 0.405046 8.2:+81582120 None:intergenic
AAAGGCTGTGGTTTATTGTC+AGG 0.408788 8.2:-81581910 MS.gene39347:CDS
CCGAAGACTTCCTCAAGAAA+TGG 0.408850 8.2:-81582120 MS.gene39347:CDS
ATGCTAAGATGAATTGGAAT+AGG 0.409960 8.2:+81586746 None:intergenic
AGACGAAACTGATAGAATTA+CGG 0.412288 8.2:-81586789 MS.gene39347:CDS
TGTACTAAACTCGGTGACTA+TGG 0.413767 8.2:-81588423 MS.gene39347:CDS
CAAATTTGCGGCAAAGTTCA+AGG 0.417106 8.2:+81583270 None:intergenic
ACCTACTAATCCATCTAATC+GGG 0.417903 8.2:-81582075 MS.gene39347:CDS
GACGATAAGACCTCCCTTCA+AGG 0.419033 8.2:+81583765 None:intergenic
AAATCGGTAATCTTCCTTCC+TGG 0.419121 8.2:+81587424 None:intergenic
GCTGACAAAGCTGGATTTGA+TGG 0.421276 8.2:-81583891 MS.gene39347:CDS
TTGCCCCAGAGCAAGGTTAC+CGG 0.423029 8.2:-81582573 MS.gene39347:CDS
GTATTCAAAGAGCATTTAGA+TGG 0.423316 8.2:-81583242 MS.gene39347:CDS
ACGACTATGGAAAGCTTAAT+CGG 0.423389 8.2:-81588471 MS.gene39347:CDS
CTTAGCATTTATTCTCCAAA+TGG 0.424920 8.2:-81586731 MS.gene39347:intron
CTGCAGCTAATTCGGCTTGA+AGG 0.425382 8.2:+81582240 None:intergenic
CTTATCGTCTGCAACATCCT+TGG 0.426910 8.2:-81583751 MS.gene39347:CDS
TTTATGCCAGCACGGTTAGA+TGG 0.427096 8.2:+81582029 None:intergenic
TTATCGTCTGCAACATCCTT+GGG 0.428575 8.2:-81583750 MS.gene39347:CDS
ACCTGAGGGGTCCACTTCCC+TGG 0.428644 8.2:+81584018 None:intergenic
CCCCGATTAGATGGATTAGT+AGG 0.432018 8.2:+81582074 None:intergenic
TGAATACACGTTCAAATTTG+CGG 0.432203 8.2:+81583258 None:intergenic
CCGGCAAATCAATCAAAGTT+AGG 0.433564 8.2:+81585167 None:intergenic
GGAGATGAGAACAGAGTTCA+GGG 0.433664 8.2:-81588252 MS.gene39347:intron
TACTGCATCAACTGAAGCTT+CGG 0.433786 8.2:+81582510 None:intergenic
TGTCCAGGTGAGAGGACATT+TGG 0.439687 8.2:-81583917 MS.gene39347:intron
CGTCGCCGTCGTCGGTGGCC+AGG 0.444062 8.2:-81588349 MS.gene39347:intron
AGACCAATTGCTGTTGATGC+GGG 0.446141 8.2:-81583404 MS.gene39347:CDS
AGCAAGGGATGAAATTGATT+CGG 0.449058 8.2:-81581033 MS.gene39347:CDS
TTTATCAAAGATGTTATACT+AGG 0.449754 8.2:+81583470 None:intergenic
GTCTCCGGTAACCTTGCTCT+GGG 0.449780 8.2:+81582569 None:intergenic
GAAGACAACAATGTTTCAAA+AGG 0.451865 8.2:-81581073 MS.gene39347:CDS
GTTGCTATGTCCTGGTTGGC+TGG 0.453996 8.2:+81584061 None:intergenic
GCACGGTTAGATGGATTAGT+AGG 0.466688 8.2:+81582038 None:intergenic
ATTTGGCGTGCTGACAAAGC+TGG 0.467319 8.2:-81583900 MS.gene39347:CDS
ACAGGCGACCAGGAGGAGAT+AGG 0.469255 8.2:-81582720 MS.gene39347:intron
CTGGCTAAGTGGCCATAGTC+AGG 0.469474 8.2:+81583635 None:intergenic
AATCAGAGATGGATCATCTT+GGG 0.469804 8.2:-81583427 MS.gene39347:CDS
GACTTTCTACCTAGAGGATC+AGG 0.470180 8.2:-81588196 MS.gene39347:intron
GCATCAACTGAAGCTTCGGC+TGG 0.470576 8.2:+81582514 None:intergenic
GATGGATTAGTAGGTTGAGC+TGG 0.471722 8.2:+81582083 None:intergenic
GAATCAGAGATGGATCATCT+TGG 0.476439 8.2:-81583428 MS.gene39347:CDS
GAACTTGTGAGAAAATCAAT+AGG 0.478284 8.2:-81582379 MS.gene39347:CDS
CAACTTCATAAGATAGAAGA+AGG 0.481307 8.2:-81587478 MS.gene39347:CDS
GGCCACTTAGCCAGTAAAAT+GGG 0.483415 8.2:-81583626 MS.gene39347:CDS
ATACTCAACCTCCTTGCGCC+TGG 0.484858 8.2:+81583670 None:intergenic
TGTCTTCTCTCCATCACGGT+AGG 0.486322 8.2:+81581089 None:intergenic
TACGTAAAGCAGCAGGAAGC+TGG 0.489120 8.2:+81582677 None:intergenic
TTATGCAGCGTTGGTACGCC+AGG 0.489375 8.2:-81586819 MS.gene39347:intron
ACAGAGTTCAGGGAAATCTT+CGG 0.489500 8.2:-81588242 MS.gene39347:intron
CTTCCTTCCGTCGCCGTCGT+CGG 0.492244 8.2:-81588357 MS.gene39347:CDS
AGAGAAAGATGTTTGTCAAT+CGG 0.497168 8.2:+81582649 None:intergenic
AGAGTAAGAAGACAACTATT+CGG 0.503106 8.2:-81582184 MS.gene39347:CDS
CACTTAATTGCCCCAGAGCA+AGG 0.512092 8.2:-81582580 MS.gene39347:CDS
GGCTATATCTCCAGCCAACC+AGG 0.515519 8.2:-81584071 MS.gene39347:CDS
CTGGGGCAATTAAGTGAGGC+TGG 0.517726 8.2:+81582587 None:intergenic
TATGGAAAGCTTAATCGGCT+TGG 0.517895 8.2:-81588466 MS.gene39347:CDS
GTTTATTGTCACAGGCGACC+AGG 0.522732 8.2:-81582730 MS.gene39347:intron
TGCAGCTAATTCGGCTTGAA+GGG 0.525361 8.2:+81582241 None:intergenic
GATTGTGACGAGGCGTCCAT+TGG 0.525514 8.2:-81587506 MS.gene39347:CDS
AATGACGATCGTTACGGTGA+GGG 0.526248 8.2:-81582008 MS.gene39347:CDS
GCTAATTCGGCTTGAAGGGT+AGG 0.528310 8.2:+81582245 None:intergenic
CATCAACTGAAGCTTCGGCT+GGG 0.528711 8.2:+81582515 None:intergenic
GGCATAAATGACGATCGTTA+CGG 0.532941 8.2:-81582014 MS.gene39347:CDS
TCCAGGGAAGTGGACCCCTC+AGG 0.537660 8.2:-81584019 MS.gene39347:intron
GAATGGTGTCGGACACACTC+AGG 0.542112 8.2:-81581944 MS.gene39347:CDS
CATCAGAAGTTGCTATGTCC+TGG 0.543250 8.2:+81584053 None:intergenic
GTCTTCTCTCCATCACGGTA+GGG 0.543606 8.2:+81581090 None:intergenic
GAAGACAACTATTCGGCTTG+TGG 0.544489 8.2:-81582177 MS.gene39347:CDS
TGTGAAGAGAGTGGTGTCAG+AGG 0.544625 8.2:-81582620 MS.gene39347:CDS
GAGACCAATTGCTGTTGATG+CGG 0.545777 8.2:-81583405 MS.gene39347:CDS
GAAAGAGAACCTGATCCTCT+AGG 0.546071 8.2:+81588187 None:intergenic
CGAAGAACTTGAATCAGAGA+TGG 0.546993 8.2:-81583438 MS.gene39347:CDS
GACCAATTGCTGTTGATGCG+GGG 0.549430 8.2:-81583403 MS.gene39347:CDS
CCACCGACGACGGCGACGGA+AGG 0.550441 8.2:+81588354 None:intergenic
ATGACGATCGTTACGGTGAG+GGG 0.551047 8.2:-81582007 MS.gene39347:CDS
TTATATATCCTATCTCCTCC+TGG 0.551940 8.2:+81582712 None:intergenic
CCTTGCAAAACTGCTCTCAC+AGG 0.554182 8.2:-81583594 MS.gene39347:intron
CCAGCTTTCTCCATTTCTTG+AGG 0.555810 8.2:+81582110 None:intergenic
CACACAAATAGGAAAGAAAG+AGG 0.557997 8.2:-81581850 MS.gene39347:intron
CAACAAGCTTGTACTAAACT+CGG 0.559054 8.2:-81588432 MS.gene39347:CDS
CTAATTCGGCTTGAAGGGTA+GGG 0.560321 8.2:+81582246 None:intergenic
CTAACTTTGATTGATTTGCC+GGG 0.561184 8.2:-81585166 MS.gene39347:CDS
TTCAAAGAGCATTTAGATGG+AGG 0.561465 8.2:-81583239 MS.gene39347:CDS
TATCAAAATTTCCAGGGAAG+TGG 0.563515 8.2:-81584029 MS.gene39347:CDS
GGTCACTATTCCAAAGGCTG+TGG 0.564280 8.2:-81581922 MS.gene39347:CDS
CCTTCCGTCGCCGTCGTCGG+TGG 0.564731 8.2:-81588354 MS.gene39347:CDS
TCAAATGTGTCATCCTACAT+AGG 0.567396 8.2:-81581966 MS.gene39347:CDS
GAGTCCCTTCAATCAGTCTC+CGG 0.567399 8.2:+81582554 None:intergenic
GCCGAGTTTCTTCATAGACC+AGG 0.569190 8.2:-81587442 MS.gene39347:CDS
TCAAAGAGCATTTAGATGGA+GGG 0.572110 8.2:-81583238 MS.gene39347:intron
TGATGCTATCAAAATTTCCA+GGG 0.575484 8.2:-81584035 MS.gene39347:CDS
GGAAGCTTACGTAAAGCAGC+AGG 0.575667 8.2:+81582670 None:intergenic
CCTCAAGAAATGGAGAAAGC+TGG 0.577336 8.2:-81582110 MS.gene39347:CDS
ATCCTACATAGGAATGGTGT+CGG 0.589525 8.2:-81581955 MS.gene39347:CDS
GGACAAAGGAACTAATGCAT+TGG 0.589827 8.2:-81583870 MS.gene39347:CDS
AGAAGTTGCTATGTCCTGGT+TGG 0.591320 8.2:+81584057 None:intergenic
CTGGCCACCGACGACGGCGA+CGG 0.594779 8.2:+81588350 None:intergenic
TTTGCGGCAAAGTTCAAGGA+TGG 0.594840 8.2:+81583274 None:intergenic
ACTAATCCATCTAACCGTGC+TGG 0.596233 8.2:-81582035 MS.gene39347:CDS
TCCTGGTCTATGAAGAAACT+CGG 0.599253 8.2:+81587441 None:intergenic
AATGGTGTCGGACACACTCA+GGG 0.601246 8.2:-81581943 MS.gene39347:CDS
CGGTACCTGGCCACCGACGA+CGG 0.601628 8.2:+81588344 None:intergenic
TTATGAAGTTGTAACACCAA+TGG 0.601894 8.2:+81587490 None:intergenic
TAAGTGGCCATAGTCAGGAC+TGG 0.601929 8.2:+81583640 None:intergenic
ATGTTAACTGTCCAGGTGAG+AGG 0.603386 8.2:-81583925 MS.gene39347:intron
TATTCGGCTTGTGGATATGG+AGG 0.603654 8.2:-81582168 MS.gene39347:CDS
ATTAGCTGCAGCTACAACTG+AGG 0.604264 8.2:-81582228 MS.gene39347:CDS
TGTGTCATCCTACATAGGAA+TGG 0.605269 8.2:-81581961 MS.gene39347:CDS
CGTCTGCAACATCCTTGGGT+CGG 0.605481 8.2:-81583746 MS.gene39347:CDS
GGTTACCGGAGACTGATTGA+AGG 0.605725 8.2:-81582559 MS.gene39347:CDS
GTCCGACACCATTCCTATGT+AGG 0.606800 8.2:+81581953 None:intergenic
TTGAACTATATAAAGCAGCA+AGG 0.610358 8.2:-81581049 MS.gene39347:CDS
ATACTGATATGATTGTTGCC+AGG 0.611071 8.2:-81583688 MS.gene39347:CDS
TATGATTGTTGCCAGGCGCA+AGG 0.611351 8.2:-81583681 MS.gene39347:CDS
GTTACCGGAGACTGATTGAA+GGG 0.613786 8.2:-81582558 MS.gene39347:CDS
ACTTCATAAGATAGAAGAAG+GGG 0.619742 8.2:-81587476 MS.gene39347:CDS
TCTTCAGAATGTGAAGAGAG+TGG 0.620832 8.2:-81582629 MS.gene39347:CDS
AGACGAAGACCCTACCGTGA+TGG 0.621898 8.2:-81581099 MS.gene39347:CDS
ACCAATTGCTGTTGATGCGG+GGG 0.622770 8.2:-81583402 MS.gene39347:intron
TGCTCTGGGGCAATTAAGTG+AGG 0.623821 8.2:+81582583 None:intergenic
TGAACTATATAAAGCAGCAA+GGG 0.626349 8.2:-81581048 MS.gene39347:CDS
AAGCTGGATTTGATGGACAA+AGG 0.627483 8.2:-81583884 MS.gene39347:CDS
GGGTTGACTAAAGTTGCTGT+AGG 0.631196 8.2:-81585145 MS.gene39347:intron
TAACTTTGATTGATTTGCCG+GGG 0.635039 8.2:-81585165 MS.gene39347:CDS
AGAGTGGTGTCAGAGGCAGA+TGG 0.635211 8.2:-81582613 MS.gene39347:CDS
AACTTCATAAGATAGAAGAA+GGG 0.635239 8.2:-81587477 MS.gene39347:CDS
CGATTTACTACACCGACCCA+AGG 0.635734 8.2:+81583734 None:intergenic
AAATTGCAGGGATTGTGACG+AGG 0.638817 8.2:-81587516 MS.gene39347:intron
GCAGGCTAAACAACTATCAG+AGG 0.640315 8.2:-81581126 MS.gene39347:intron
AAAATCAATAGGTGAAACTG+AGG 0.644678 8.2:-81582368 MS.gene39347:intron
GGAGGCTTCGTATCTTACTG+TGG 0.644824 8.2:-81582150 MS.gene39347:CDS
TGGAGTCGGTAATTCGGGCA+CGG 0.645097 8.2:-81583496 MS.gene39347:CDS
ACTCAGGGTCACTATTCCAA+AGG 0.654601 8.2:-81581928 MS.gene39347:CDS
TTGTTGTCTTCTCTCCATCA+CGG 0.655926 8.2:+81581085 None:intergenic
GGACGTGACTTTCTACCTAG+AGG 0.662283 8.2:-81588202 MS.gene39347:CDS
CTACAACTGAGGCTCTAGAG+AGG 0.662304 8.2:-81582217 MS.gene39347:CDS
AGTTTCTTCATAGACCAGGA+AGG 0.662508 8.2:-81587438 MS.gene39347:CDS
AAATGACGATCGTTACGGTG+AGG 0.670553 8.2:-81582009 MS.gene39347:CDS
CCTACTAATCCATCTAATCG+GGG 0.671752 8.2:-81582074 MS.gene39347:CDS
GATCGTCATTTATGCCAGCA+CGG 0.674076 8.2:+81582021 None:intergenic
GATTGTTGCCAGGCGCAAGG+AGG 0.689806 8.2:-81583678 MS.gene39347:CDS
ACAGCAACTTTAGTCAACCC+CGG 0.696272 8.2:+81585148 None:intergenic
TATTGTCACAGGCGACCAGG+AGG 0.697345 8.2:-81582727 MS.gene39347:intron
ACTAAACTCGGTGACTATGG+CGG 0.703039 8.2:-81588420 MS.gene39347:CDS
TTGTCTTCTTACTCTCATCG+CGG 0.704669 8.2:+81582192 None:intergenic
TCTCCGGTAACCTTGCTCTG+GGG 0.721661 8.2:+81582570 None:intergenic
GGTACATTAACATACCTGAG+GGG 0.808362 8.2:+81584005 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr8.2 gene 81581016 81588496 81581016 ID=MS.gene39347
chr8.2 mRNA 81581016 81588496 81581016 ID=MS.gene39347.t1;Parent=MS.gene39347
chr8.2 exon 81588350 81588496 81588350 ID=MS.gene39347.t1.exon1;Parent=MS.gene39347.t1
chr8.2 CDS 81588350 81588496 81588350 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81588197 81588260 81588197 ID=MS.gene39347.t1.exon2;Parent=MS.gene39347.t1
chr8.2 CDS 81588197 81588260 81588197 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81587422 81587529 81587422 ID=MS.gene39347.t1.exon3;Parent=MS.gene39347.t1
chr8.2 CDS 81587422 81587529 81587422 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81586732 81586833 81586732 ID=MS.gene39347.t1.exon4;Parent=MS.gene39347.t1
chr8.2 CDS 81586732 81586833 81586732 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81585146 81585196 81585146 ID=MS.gene39347.t1.exon5;Parent=MS.gene39347.t1
chr8.2 CDS 81585146 81585196 81585146 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81584974 81585035 81584974 ID=MS.gene39347.t1.exon6;Parent=MS.gene39347.t1
chr8.2 CDS 81584974 81585035 81584974 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81584020 81584110 81584020 ID=MS.gene39347.t1.exon7;Parent=MS.gene39347.t1
chr8.2 CDS 81584020 81584110 81584020 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81583868 81583932 81583868 ID=MS.gene39347.t1.exon8;Parent=MS.gene39347.t1
chr8.2 CDS 81583868 81583932 81583868 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81583595 81583789 81583595 ID=MS.gene39347.t1.exon9;Parent=MS.gene39347.t1
chr8.2 CDS 81583595 81583789 81583595 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81583403 81583522 81583403 ID=MS.gene39347.t1.exon10;Parent=MS.gene39347.t1
chr8.2 CDS 81583403 81583522 81583403 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81583239 81583309 81583239 ID=MS.gene39347.t1.exon11;Parent=MS.gene39347.t1
chr8.2 CDS 81583239 81583309 81583239 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81582513 81582738 81582513 ID=MS.gene39347.t1.exon12;Parent=MS.gene39347.t1
chr8.2 CDS 81582513 81582738 81582513 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81582369 81582419 81582369 ID=MS.gene39347.t1.exon13;Parent=MS.gene39347.t1
chr8.2 CDS 81582369 81582419 81582369 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81581851 81582282 81581851 ID=MS.gene39347.t1.exon14;Parent=MS.gene39347.t1
chr8.2 CDS 81581851 81582282 81581851 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
chr8.2 exon 81581016 81581144 81581016 ID=MS.gene39347.t1.exon15;Parent=MS.gene39347.t1
chr8.2 CDS 81581016 81581144 81581016 ID=cds.MS.gene39347.t1;Parent=MS.gene39347.t1
Gene Sequence

>MS.gene39347

ATGACGACTATGGAAAGCTTAATCGGCTTGGTTAACAGAATCCAACAAGCTTGTACTAAACTCGGTGACTATGGCGGTTCTGATAGCAACAACACTTTTTCTTCTCTTTGGGAAGCTCTTCCTTCCGTCGCCGTCGTCGGTGGCCAGGTACCGTTGCTTCTAGAAACTTCTAAATATTGAATTTCATTTTATTTTATGCTATTTCTTATTTTATTTATTTTTGGAGATGAGAACAGAGTTCAGGGAAATCTTCGGTTTTGGAGAGTATCGTTGGACGTGACTTTCTACCTAGAGGATCAGGTTCTCTTTCTTTTTCCTTTTTTATTTTTACATTTTTTCGTTTTTATTTTTTTTATTTTTTTATTTTGGTTTGTGGAGTATGTTAGTTATGCCTTCAGATAAACAACTTATTTTGCAGCTTATAACATAAACGCTTATCATATGAACAAGTTATTTCTATAATATAAGGTAAAATAGCGAAATTGTTTTCGTATTTTAAGAGGTTTTCGTAAGCTATCCTGAAGAGTTTATGGAAATAATTTGATAATAGCTTATGCTATGAAATACTGACACATGAGGTTTTGGGTAGCTCAACTGGTTTTAGCTATAAGCGACAAGGAGGAGTTAGAGGTTCTGAGTTCAAACACGATAAAGGAGAAGAAATTTATCTAACAACTAATTACTAATATTGGCCATTAAAAGAAAGATGGAATACTGACACGCTTTTTATAAAAAAGTGCTGGTGCTACATAAAGCTTATCAACATGTCATAAGCTGTTTTCATAACCTCTACCAAACAATATCACAAGTGTTTATGCTAGTAGATAAGTTCAAATAAACCAATATAAACGGGCTCTTGGTCTATTTAGGTAATTTAGTTATATTTTTTTGTTGTTGCTTATGATTTGTAAGTTCATGTGTGTGTTCGTGAATTGAATTTGAAGCGGAATTCGGATTTAAATTGCAGGGATTGTGACGAGGCGTCCATTGGTGTTACAACTTCATAAGATAGAAGAAGGGGAAAAAGAGTATGCCGAGTTTCTTCATAGACCAGGAAGGAAGATTACCGATTTTGGTAAAGTAGTAGTCTAGTTAGTTACATCTATATTCTATTTTCTTTCTTATTTTTTGTTTCATTAATATCTGTTTAGTACTTCATAAGTCGAAGCTTTTGATCAGTTACTCGAGATTCGTAGTGTAACGCATGATAGCATATTCACTAATTATGGGAATAAGATACCCTGTTTTTTAAGACATGATACCCTTCTTTTAGTTTCTTCTATTAATCAATGGGTTGTTTGTAACTGAAGTTGAATACTCCTAACTCGTTAGTGAACCTTTTGAACTTGTTTGGTTTCATACCTGTATATCATCTTAATCTGCCTCTATTATGTTTTAATTGTTTGCATATGTGTTTCTTCCGTAATTTTGGTGGCAGGTTTTATGTTTTACTCTATTAGACTCGTTCACATTTTTACGTAATCATAGCATTAATCTTTCAAGTGATTTGTTTTTCATCATTATTCATGGACTAATAAAATAATAATCAAGTTTACCAGTGCGATAATTTGCATGCTATTGCTAAATCTTTTTTTAGTGTAGTTTATATATATGAAAATAATGTTTATGTCTTTATTTTTTAATTCTGGTTTTCCTTATGCAGCGTTGGTACGCCAGGAAATTCAAGACGAAACTGATAGAATTACGGGAAAGACAAAGCAAATATCTCCTATTCCAATTCATCTTAGCATTTATTCTCCAAATGGTACGCTTCAATTGTTGCTGTCTTTTTCTGAAAATTTTAGTAGATGTTTGCTGTAAACTCACGTTTGACACTTCCATAGCTTACTTTTGAATTAAAGTAATTTTTGGTATTGAAATATAGAAAATAGTACAAAACAGAGGTATTGGTATTTTTTTTACCTCCCAAGTAGTTTTCTAATGAATATAACAATTCCATACACTGTGTGCTTGTTGGCGAAGTAAGCTCCGTCACATAAGTCATTGGCTGAACACTATAAGGAATCAACAATCCAATTCTTTAAAATGAAAGATGCAAAATCTTCTGCTCGAAACAAATCCTTGATTATTTTCCATCCAAACCTCTGAATCTGCTTCATGAAATTCCCTCCCGCATTTCTCTGTGAAGTTGAGAATGTATAAGACAAAATTTTCCATGTTTGCTGGATAAGCCCAAGGCTATCAGAAAATTGCTGCATCTCATAAGCTAATAACATATAGCTGAACACTTATACTGGGCTAATGTTTTCCTTTCTATTCAATCAAGTCATCACTCATTATTATTAGTTAAATTCATCAATTTTGTTGCTATAATTCAAATGTAAATATGTAATTAAAACCTTAAATGGAACCCTAGGGTAGCAATATTCTTAATCAATTGTGCTATATGACTGTAAATTTTTGGGTGAGGTGCAAGGGATTGGAGTTTAGGGGGGATAGGGATCTTGAAGAATTTCGGGGGCCCTAGGGGGAAGGCTTTCCACTCCTTCCTTACATGCAGCCTGCACATAGCATCAAGGATCTGAAAGGAAACAGAACTGCAAACTCTCCCACCTGCTAGTGAAGATAAACAAAAAGAGTCAAGTAAAGGCAGAAGTGATAAAAAAAATTAAAAAACCAGAGGGATCCCTCAATCAAAAAATCCTGGATAATAAAAGTTTCTTGAAACCTTGCCAAATGCTACTACATATTCAGGTGACCAGGGAAGAGTTAGAAACGAAATATCGAATAAAAAAGGGAAAATTTCAAAGAAAATAATGTCAACAATCTATATAAAACTGGAACCATTTAACCAAGGAAAGGAAGAATAGTCTAGAGTGATAAATTTGCCAAAGACTAGAATAACACGAGTTTTTGGTTGGACATCCTAAATATTTTGATAAATAATATGGTTACTTAGTAGCTGTTTGCTTCCATTTTAATGGGGCGGGAGGATTAGCACCTCTTTTAGTAGGATCTACCTAGTCACCTGCTCTCTAGGTAGTTGCAACTACCTCTTTTGCAATTTCTTGAATGTATTTTTTAGTGTTTCTTTAGTTTCACGTGTTTAAGGTTATAGAACAGCAATTCGAGAAAGGATTTGAATATTTTAATTTTAATTTACTTGTATTTTTTTCATTTGATAAAGGTTTAAGTGTATATTTAATATTTATTGTTTAACTTTGCATTTTGTTGATGTATAACTATATATGGATCAGAAGAGACTAGTTATTCAACGGTTTTGAAGTCTACCAATCAGCATTTTGTGATTCCTAATTCAGTTTCTGTTTTTTTGAGCAGTTGTCAACCTAACTTTGATTGATTTGCCGGGGTTGACTAAAGTTGCTGTAGGTATAATTTGCATTCAACTGTTGCTATAAATTTATTTTAAATTAAAAAAATATATATGTTGTTCAAAATGATAACCTTCAGTGAAATGTTCACAACTATGTCATTTTCAGAAGGACAGTCTGAGAGTATTGTTCAAGATATTGAAAATATGGTTCGATCTTTTATTGACAAGGTAAAAGGAAGTTGTTCCATTTTATTTTAATTTATCCTCATTATTCTGGTTGAATGTTAATTTGGATGATGTCCCCATAATATTGTTTTAAGGAAAATTTCGATGCTATATGGGAATGAATGTTTGATGGTAATTTGGTAAAGAGCCAATTAGAAAGTAAGTAGTGTTGCCATCTTGGATTGATGAAAATGATAAATTTGACGAGTGGCCACACAAGACGAGACACTACTAGGAATGAAGTCTAAATGGTTTAGAGAGAATGTAGGGATAGCACCTGTTATAGAGAATATGGGAGTCTCGTTTTTTATGGTCTATGTATGTCATAGATGGAGGATAGTCAGATCACTAGAGGTAGAGGAAGACCTAGAAAAAAACATAAGAGAAACTATTAAGAATATTTAGAGACCAATGAGTTGGATCCTAATATGGTTTGTGATAGAACATTATAGTGTAATTTGATCCATGTAGCCGACCCCACTTAGTGAGATAAGGTTTGGTGGTTGTTGTTGTTGTTGTTGTGTATGTATGGAGAAGACCCATAGTTCATAGAAGCAATACCAATGAGGGTAAATTAAAGGGAGAATCATTTAATAGGTAGAGATATAAAGACCCAAAAAATTGTAAGCCAAACCATTAAGAGAGCTTTTGATTAAAAGGTCCACATTAGACATTAGACATGGTTCACAGTTGGACATTACGGTGTTGACTGTTGATGCTCTTTTCATTTATTTGTTTTGCATTGGGGCTACAATTATAAACTGCCTATTGTCAAAAAAAAATCTCTTACTATGCTTCTAATGGTGAATTAACAAGAGATAGCATCAAAATTCATATTTTCTTTAACCAGTTTTTGCTTTTGGCAGCCTAATTGTATCATACTGGCTATATCTCCAGCCAACCAGGACATAGCAACTTCTGATGCTATCAAAATTTCCAGGGAAGTGGACCCCTCAGGTATGTTAATGTACCATGGATTTATCGCTTCTTGCCTTTGGCCGAGTAATTGTCTGTCACTGAAATCCTTGTATGTTAACTGTCCAGGTGAGAGGACATTTGGCGTGCTGACAAAGCTGGATTTGATGGACAAAGGAACTAATGCATTGGATGTAATCTCTCTCACACTTTCTCTGGCATGATTCCTTGTATAATTGGTTTGTCCTTTCACCCGTTTTTTTCCCTTTTAGGTCCTTGAAGGGAGGTCTTATCGTCTGCAACATCCTTGGGTCGGTGTAGTAAATCGATCTCAAGCAGATATCAACAGAAATACTGATATGATTGTTGCCAGGCGCAAGGAGGTTGAGTATTTTGAAACCAGTCCTGACTATGGCCACTTAGCCAGTAAAATGGGTTCAGAGTACCTTGCAAAACTGCTCTCACAGGTTTGTATTATGGAAAGTTGTTTGCCTTTAATGAAATGTGGTATAATTTAACACTGTTTGATTATTTTGTAGCACTTGGAGTCGGTAATTCGGGCACGGATACCTAGTATAACATCTTTGATAAATAAAAGCATCGAAGAACTTGAATCAGAGATGGATCATCTTGGGAGACCAATTGCTGTTGATGCGGGGGTGAGTGGTTTGCATTTGTATTTATTTCAGGATGAGTATAGGCTTTCTATACATCCATTATAATTACATTTTAACTGTATCTTTTTCTTTTAGGCTCAACTATACACCATCCTTGAACTTTGCCGCAAATTTGAACGTGTATTCAAAGAGCATTTAGATGGAGGGTAAATATATTCTATCATGCTATACTTTATCTCTATTCTCTGCCTTTTCTGGGTGGACAGCATGTATATTGTATAACTTGACGTTTTGGGTCGATACTTGGGTAAAAAATGCTTTTTTTATTCCCGCCTTCATGAAATAGCTTCTGCAAAAAGAAAAAAGAAAAAATATTTTAATGAAAGAAAGTTATTGCTTAAAATGCTCTTACTTATTTGATTCTTTTTACAGTAACCATCAACCATGGATTAAATTGGACGTCCAAACTCAATAGTGTTGTATTTGTTGAGTTAGCTAAATCCAAATTTAATTCTTAGTTATGTTTTCACTGCTGGTTCTTTCTGAGAAAAACTTTTCATACTGCTATGCAAAAGATGAAAGATTTAATATACCAAATGAATATTTAAGCAATTTGGAATAATTTTGTGAAGTCAGCTTTTATGTGATAGACAAGTTGATAGGTTCTCTTTGGTTACACAATCATCTCTCTTGTTTATTGTCACAGGCGACCAGGAGGAGATAGGATATATAATGTGTTTGACAACCAGCTTCCTGCTGCTTTACGTAAGCTTCCGATTGACAAACATCTTTCTCTTCAGAATGTGAAGAGAGTGGTGTCAGAGGCAGATGGTTACCAGCCTCACTTAATTGCCCCAGAGCAAGGTTACCGGAGACTGATTGAAGGGACTCTCAGTTACTTTAGAGGCCCAGCCGAAGCTTCAGTTGATGCAGTAAGCAGCCCTAGTTGTTTTCTTTTTTTCATTTGTTTTAAAGCTAACATGAACGAAAATACTAAGCTATATATCTCTATGATCTGGATGTAGGTTCACTTTGTATTAAAAGAACTTGTGAGAAAATCAATAGGTGAAACTGAGGTAAAAATTTTAGATTCATTTTGCAGTAACCCTGTTTGAAATGTACTTAGAATAGTCAATAAAGCACAAATTGTGTCTCTCTACAGGAATTGAGACGTTTCCCTACCCTTCAAGCCGAATTAGCTGCAGCTACAACTGAGGCTCTAGAGAGGTTCCGCGATGAGAGTAAGAAGACAACTATTCGGCTTGTGGATATGGAGGCTTCGTATCTTACTGTGGATTTCTTCCGAAGACTTCCTCAAGAAATGGAGAAAGCTGGTACTCCAGCTCAACCTACTAATCCATCTAATCGGGGTAATCCAGATCAACCTACTAATCCATCTAACCGTGCTGGCATAAATGACGATCGTTACGGTGAGGGGCATTTTAGAAGGATTGGATCAAATGTGTCATCCTACATAGGAATGGTGTCGGACACACTCAGGGTCACTATTCCAAAGGCTGTGGTTTATTGTCAGGTCAGAGAAGCAAAACAGTCATTGCTGAACTATTTCTACACACAAATAGGAAAGAAAGAGGTACTCTCTCTCTCTCTCTCTCCCTCTCTCAGTGAAAAGCTCACTTCTCTTCCCTATCCTTGCAATTGCAATTTTAGATTTATCTTTCAAGTCCAACCTCTTTTGGTAACAAAGACATTAAAAACAATCGAATAAAATACAACCATTTTTTTTATAAATGATGCGGATTAAGTGTCTTGACAATGGAAGGAGAATAGTCTTGGGTTTGGATTGGATTTCTTTTCAAACTGATATTCTACTAGGAGTTTGTTGACTAAAATGAATACATAGAAATCATTTATTTGATCACATAGCCAGTGTCCCCATAAGAATTTTTCAATGCCGTCTTAGTGTATTTTTGTAAGCATGATGAGCACAAAAATTATAAGCATGGTATAAAATGTTGAACTTTCTCATTTTGTCCTTACTGTTTCATGCTAAATACAATTGCTCTCTAGTCTACATCTACATTAATACAAACATTGAACGTGCCCTATAATATCCTAGCATAAAATCTGCCATATACCACTACATGGATTTGGTTCCTCTAAAGTTAACTCACTTTAAAAGGTACACTAACATTTGAATCTATAACTGTGTAAATCCACTCTTCACAAAACGAACTAGGCGAATGGCTAATTATGAAAACATACATTTATGCTCATAATACCCGTTTTGTTGTTTTCATTATATCCCTAAGATCTTGTCTGCTATAATGTTGGTGCAGGCTAAACAACTATCAGAGGTATTAGACGAAGACCCTACCGTGATGGAGAGAAGACAACAATGTTTCAAAAGGCTTGAACTATATAAAGCAGCAAGGGATGAAATTGATTCGGTTTCTTGGGTACGATGA
Protein sequence

>MS.gene39347.t1

MTTMESLIGLVNRIQQACTKLGDYGGSDSNNTFSSLWEALPSVAVVGGQSSGKSSVLESIVGRDFLPRGSGIVTRRPLVLQLHKIEEGEKEYAEFLHRPGRKITDFALVRQEIQDETDRITGKTKQISPIPIHLSIYSPNVVNLTLIDLPGLTKVAVEGQSESIVQDIENMVRSFIDKPNCIILAISPANQDIATSDAIKISREVDPSGERTFGVLTKLDLMDKGTNALDVLEGRSYRLQHPWVGVVNRSQADINRNTDMIVARRKEVEYFETSPDYGHLASKMGSEYLAKLLSQHLESVIRARIPSITSLINKSIEELESEMDHLGRPIAVDAGAQLYTILELCRKFERVFKEHLDGGRPGGDRIYNVFDNQLPAALRKLPIDKHLSLQNVKRVVSEADGYQPHLIAPEQGYRRLIEGTLSYFRGPAEASVDAVHFVLKELVRKSIGETEELRRFPTLQAELAAATTEALERFRDESKKTTIRLVDMEASYLTVDFFRRLPQEMEKAGTPAQPTNPSNRGNPDQPTNPSNRAGINDDRYGEGHFRRIGSNVSSYIGMVSDTLRVTIPKAVVYCQVREAKQSLLNYFYTQIGKKEAKQLSEVLDEDPTVMERRQQCFKRLELYKAARDEIDSVSWVR