Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068015.t1 | RHN62652.1 | 99.3 | 765 | 5 | 0 | 1 | 765 | 1 | 765 | 0.00E+00 | 1468.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068015.t1 | Q8S944 | 71.7 | 759 | 196 | 5 | 9 | 757 | 31 | 780 | 1.6e-297 | 1023.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068015.t1 | A0A396IAM3 | 99.3 | 765 | 5 | 0 | 1 | 765 | 1 | 765 | 0.0e+00 | 1468.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene059333 | MS.gene068015 | PPI |
| MS.gene39665 | MS.gene068015 | PPI |
| MS.gene010407 | MS.gene068015 | PPI |
| MS.gene010879 | MS.gene068015 | PPI |
| MS.gene013409 | MS.gene068015 | PPI |
| MS.gene059335 | MS.gene068015 | PPI |
| MS.gene06506 | MS.gene068015 | PPI |
| MS.gene06504 | MS.gene068015 | PPI |
| MS.gene02205 | MS.gene068015 | PPI |
| MS.gene049382 | MS.gene068015 | PPI |
| MS.gene01221 | MS.gene068015 | PPI |
| MS.gene39347 | MS.gene068015 | PPI |
| MS.gene002248 | MS.gene068015 | PPI |
| MS.gene041137 | MS.gene068015 | PPI |
| MS.gene028493 | MS.gene068015 | PPI |
| MS.gene050237 | MS.gene068015 | PPI |
| MS.gene068015 | MS.gene047680 | PPI |
| MS.gene056130 | MS.gene068015 | PPI |
| MS.gene053588 | MS.gene068015 | PPI |
| MS.gene013407 | MS.gene068015 | PPI |
| MS.gene90230 | MS.gene068015 | PPI |
| MS.gene71381 | MS.gene068015 | PPI |
| MS.gene26060 | MS.gene068015 | PPI |
| MS.gene022695 | MS.gene068015 | PPI |
| MS.gene068015 | MS.gene002248 | PPI |
| MS.gene059331 | MS.gene068015 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068015.t1 | MTR_4g093750 | 99.069 | 752 | 5 | 1 | 1 | 750 | 1 | 752 | 0.0 | 1521 |
| MS.gene068015.t1 | MTR_4g093750 | 99.104 | 670 | 4 | 1 | 1 | 670 | 1 | 668 | 0.0 | 1347 |
| MS.gene068015.t1 | MTR_2g009690 | 83.026 | 760 | 120 | 3 | 11 | 763 | 20 | 777 | 0.0 | 1281 |
| MS.gene068015.t1 | MTR_5g009150 | 38.632 | 497 | 285 | 4 | 15 | 499 | 4 | 492 | 2.34e-104 | 333 |
| MS.gene068015.t1 | MTR_4g098920 | 40.323 | 496 | 275 | 6 | 14 | 497 | 3 | 489 | 3.84e-100 | 322 |
| MS.gene068015.t1 | MTR_8g099550 | 38.540 | 493 | 282 | 5 | 15 | 495 | 4 | 487 | 1.30e-99 | 320 |
| MS.gene068015.t1 | MTR_8g027675 | 37.805 | 492 | 286 | 5 | 15 | 495 | 4 | 486 | 6.41e-97 | 313 |
| MS.gene068015.t1 | MTR_8g009510 | 38.431 | 497 | 282 | 5 | 14 | 495 | 6 | 493 | 2.29e-94 | 307 |
| MS.gene068015.t1 | MTR_8g105580 | 38.134 | 493 | 285 | 6 | 13 | 495 | 5 | 487 | 5.07e-94 | 305 |
| MS.gene068015.t1 | MTR_8g105580 | 38.134 | 493 | 285 | 6 | 13 | 495 | 5 | 487 | 9.55e-94 | 305 |
| MS.gene068015.t1 | MTR_7g451010 | 39.525 | 463 | 267 | 4 | 37 | 495 | 40 | 493 | 3.16e-92 | 301 |
| MS.gene068015.t1 | MTR_1g032550 | 36.714 | 493 | 293 | 5 | 13 | 495 | 5 | 488 | 4.48e-90 | 295 |
| MS.gene068015.t1 | MTR_8g009510 | 34.518 | 394 | 245 | 4 | 106 | 495 | 2 | 386 | 8.70e-59 | 209 |
| MS.gene068015.t1 | MTR_8g087750 | 37.654 | 324 | 182 | 7 | 35 | 352 | 69 | 378 | 1.11e-45 | 174 |
| MS.gene068015.t1 | MTR_8g087770 | 34.739 | 403 | 234 | 14 | 35 | 425 | 66 | 451 | 1.82e-43 | 167 |
| MS.gene068015.t1 | MTR_4g030140 | 22.495 | 489 | 317 | 16 | 40 | 499 | 42 | 497 | 9.70e-17 | 85.5 |
| MS.gene068015.t1 | MTR_8g463270 | 30.288 | 208 | 128 | 4 | 20 | 217 | 31 | 231 | 5.41e-15 | 79.7 |
| MS.gene068015.t1 | MTR_4g094300 | 30.052 | 193 | 127 | 5 | 32 | 217 | 35 | 226 | 1.57e-13 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068015.t1 | AT4G33650 | 72.605 | 741 | 192 | 4 | 7 | 737 | 29 | 768 | 0.0 | 1085 |
| MS.gene068015.t1 | AT4G33650 | 72.237 | 742 | 194 | 4 | 7 | 737 | 29 | 769 | 0.0 | 1081 |
| MS.gene068015.t1 | AT2G14120 | 71.583 | 739 | 195 | 6 | 10 | 736 | 16 | 751 | 0.0 | 1059 |
| MS.gene068015.t1 | AT2G14120 | 71.448 | 739 | 196 | 6 | 10 | 736 | 16 | 751 | 0.0 | 1057 |
| MS.gene068015.t1 | AT2G14120 | 68.880 | 768 | 195 | 7 | 10 | 736 | 16 | 780 | 0.0 | 1042 |
| MS.gene068015.t1 | AT2G14120 | 71.508 | 723 | 191 | 6 | 10 | 720 | 16 | 735 | 0.0 | 1036 |
| MS.gene068015.t1 | AT5G42080 | 38.742 | 493 | 281 | 5 | 15 | 495 | 4 | 487 | 3.53e-100 | 322 |
| MS.gene068015.t1 | AT5G42080 | 38.742 | 493 | 281 | 5 | 15 | 495 | 4 | 487 | 3.53e-100 | 322 |
| MS.gene068015.t1 | AT3G61760 | 38.755 | 498 | 278 | 10 | 14 | 496 | 3 | 488 | 2.33e-98 | 316 |
| MS.gene068015.t1 | AT3G61760 | 38.755 | 498 | 278 | 10 | 14 | 496 | 3 | 488 | 2.90e-98 | 317 |
| MS.gene068015.t1 | AT5G42080 | 38.540 | 493 | 276 | 6 | 15 | 495 | 4 | 481 | 1.56e-97 | 315 |
| MS.gene068015.t1 | AT5G42080 | 41.589 | 428 | 231 | 4 | 15 | 432 | 4 | 422 | 3.86e-97 | 308 |
| MS.gene068015.t1 | AT3G61760 | 40.260 | 462 | 251 | 9 | 14 | 462 | 3 | 452 | 1.86e-96 | 308 |
| MS.gene068015.t1 | AT3G60190 | 37.405 | 524 | 302 | 7 | 14 | 522 | 6 | 518 | 1.28e-94 | 308 |
| MS.gene068015.t1 | AT2G44590 | 37.449 | 494 | 289 | 4 | 14 | 495 | 3 | 488 | 4.12e-93 | 303 |
| MS.gene068015.t1 | AT1G14830 | 37.602 | 492 | 288 | 5 | 14 | 495 | 6 | 488 | 2.29e-92 | 301 |
| MS.gene068015.t1 | AT2G44590 | 35.223 | 494 | 283 | 5 | 14 | 495 | 3 | 471 | 5.10e-80 | 268 |
| MS.gene068015.t1 | AT2G44590 | 35.223 | 494 | 283 | 5 | 14 | 495 | 3 | 471 | 5.24e-80 | 268 |
| MS.gene068015.t1 | AT1G60500 | 31.041 | 509 | 320 | 14 | 9 | 503 | 33 | 524 | 7.48e-49 | 183 |
| MS.gene068015.t1 | AT1G60530 | 39.493 | 276 | 144 | 7 | 9 | 275 | 30 | 291 | 1.91e-43 | 159 |
| MS.gene068015.t1 | AT1G10290 | 22.822 | 482 | 322 | 15 | 40 | 499 | 41 | 494 | 3.99e-18 | 89.7 |
| MS.gene068015.t1 | AT1G59610 | 22.614 | 482 | 323 | 14 | 40 | 499 | 41 | 494 | 1.03e-17 | 88.6 |
| MS.gene068015.t1 | AT1G53140 | 28.311 | 219 | 145 | 4 | 20 | 228 | 47 | 263 | 4.78e-12 | 70.1 |
| MS.gene068015.t1 | AT3G19720 | 29.082 | 196 | 125 | 7 | 32 | 217 | 43 | 234 | 8.51e-11 | 66.2 |
| MS.gene068015.t1 | AT3G19720 | 29.082 | 196 | 125 | 7 | 32 | 217 | 43 | 234 | 8.88e-11 | 65.9 |
Find 191 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACTTCCACCAATAAAATT+TGG | 0.140968 | 4.3:-20959427 | None:intergenic |
| ATACCTCCATTTCTATGATA+TGG | 0.216407 | 4.3:-20957732 | None:intergenic |
| CTCTAAACTCCTCACAAATA+TGG | 0.260098 | 4.3:-20956425 | None:intergenic |
| TTAGGCAAGCCAGTTGTTTC+AGG | 0.269599 | 4.3:-20962826 | None:intergenic |
| CCAAATCCGCGCTGAAATTC+AGG | 0.271521 | 4.3:+20954474 | MS.gene068015:CDS |
| AAACGCATGGATGAAGTTAT+TGG | 0.289990 | 4.3:+20957664 | MS.gene068015:CDS |
| TCATGTCTTGCAGGCTGGAC+AGG | 0.292960 | 4.3:+20956113 | MS.gene068015:intron |
| CTGAATTTCAGCGCGGATTT+GGG | 0.296468 | 4.3:-20954473 | None:intergenic |
| CCTGAATTTCAGCGCGGATT+TGG | 0.307809 | 4.3:-20954474 | None:intergenic |
| TAAGGCGATCATGCACTTCT+TGG | 0.319960 | 4.3:+20960428 | MS.gene068015:CDS |
| CGATGCTCGGAATCTGTTAC+TGG | 0.328569 | 4.3:+20955128 | MS.gene068015:CDS |
| CAAGTCTTCTACAGTTTCTA+AGG | 0.329354 | 4.3:+20959477 | MS.gene068015:CDS |
| ATGAAGAAACTCAGCTGATT+CGG | 0.331844 | 4.3:-20954422 | None:intergenic |
| CTCATTCTTTCCCTCTACTA+TGG | 0.332851 | 4.3:-20956347 | None:intergenic |
| TACTTCCACCAATAAAATTT+GGG | 0.334413 | 4.3:-20959426 | None:intergenic |
| CATATTTGTGAGGAGTTTAG+AGG | 0.340009 | 4.3:+20956426 | MS.gene068015:CDS |
| TCAGCTGATTCGGGTTTAGA+AGG | 0.366858 | 4.3:-20954412 | None:intergenic |
| ATGTATTCTACTAGCAGTTC+AGG | 0.370412 | 4.3:+20962874 | MS.gene068015:CDS |
| GTTGCAGGTCATAGAACTAT+AGG | 0.371796 | 4.3:+20954965 | MS.gene068015:intron |
| TACACCGGCTAATTCTGATT+TGG | 0.373560 | 4.3:+20954808 | MS.gene068015:CDS |
| TGAAGAAACTCAGCTGATTC+GGG | 0.376049 | 4.3:-20954421 | None:intergenic |
| TCTTCATCGCAAATCTATAT+AGG | 0.379851 | 4.3:+20961047 | MS.gene068015:CDS |
| AACCACTGGTGGATCTAGTT+GGG | 0.393292 | 4.3:+20960072 | MS.gene068015:CDS |
| TTCAACTTCTTTGCCAATTG+AGG | 0.398038 | 4.3:-20955629 | None:intergenic |
| TCTGATGGCCTCAAGATAGT+AGG | 0.401234 | 4.3:-20960306 | None:intergenic |
| TCTGACAAACCGGAGTATAA+AGG | 0.407487 | 4.3:+20955450 | MS.gene068015:CDS |
| GATCACCCATATCATAGAAA+TGG | 0.408482 | 4.3:+20957726 | MS.gene068015:CDS |
| TCATTGTCTCTGAGGGTTCA+AGG | 0.410651 | 4.3:-20957705 | None:intergenic |
| ATCGATAAAGTATTACATTC+AGG | 0.413000 | 4.3:+20959877 | MS.gene068015:CDS |
| TCCGATGCTCTTCAAATGGC+TGG | 0.413901 | 4.3:+20954836 | MS.gene068015:CDS |
| GAGGCAGATATCTCGGCTAT+TGG | 0.417224 | 4.3:+20957454 | MS.gene068015:CDS |
| ATGTCTACATCTGAGCTATC+AGG | 0.419688 | 4.3:+20956373 | MS.gene068015:CDS |
| CACATCAAGGCTGTGCTACC+AGG | 0.427513 | 4.3:+20955885 | MS.gene068015:CDS |
| TGGATTGTAGTTGGATATCA+TGG | 0.427886 | 4.3:+20955095 | MS.gene068015:intron |
| CACAGTTCGCCTGAAACAAC+TGG | 0.430116 | 4.3:+20962817 | MS.gene068015:CDS |
| AACGCATGGATGAAGTTATT+GGG | 0.431365 | 4.3:+20957665 | MS.gene068015:CDS |
| CGCTAGACTTGCCGCTACTC+TGG | 0.431697 | 4.3:-20954297 | None:intergenic |
| TCAGATCAGCCATCGTTGTA+TGG | 0.438010 | 4.3:+20957609 | MS.gene068015:intron |
| TTTGTTGACTAGTGAGATTA+CGG | 0.438850 | 4.3:-20954203 | None:intergenic |
| ATCCAAGTCTTCAATGTGCC+AGG | 0.439754 | 4.3:+20957477 | MS.gene068015:CDS |
| CGCGGATTTGGGAGAAATCA+TGG | 0.440410 | 4.3:-20954462 | None:intergenic |
| TCCTAGCGGGGCGCCGTCGT+TGG | 0.445295 | 4.3:-20954176 | None:intergenic |
| GTGCTATTCTAGATATATAG+TGG | 0.449866 | 4.3:+20955584 | MS.gene068015:intron |
| GATGCTGTGGAATCTGATAA+AGG | 0.451482 | 4.3:+20959620 | MS.gene068015:CDS |
| TAGTTGTGGCGTTCCTCAAT+TGG | 0.451776 | 4.3:+20955616 | MS.gene068015:CDS |
| CTGATGGCCTCAAGATAGTA+GGG | 0.454854 | 4.3:-20960305 | None:intergenic |
| CTCCCTCAGGTTGCCGTCGT+CGG | 0.457022 | 4.3:+20954271 | MS.gene068015:CDS |
| GAACCACTGGTGGATCTAGT+TGG | 0.459664 | 4.3:+20960071 | MS.gene068015:CDS |
| ACCGCCATACAGAATGCAAC+TGG | 0.461119 | 4.3:+20957256 | MS.gene068015:CDS |
| ATCAGGATATTCTGACAAAC+CGG | 0.462757 | 4.3:+20955440 | MS.gene068015:intron |
| TACCTCCATTTCTATGATAT+GGG | 0.466124 | 4.3:-20957731 | None:intergenic |
| CATGTCTTGCAGGCTGGACA+GGG | 0.466689 | 4.3:+20956114 | MS.gene068015:intron |
| AAGAAGTGCATGATCGCCTT+AGG | 0.473310 | 4.3:-20960426 | None:intergenic |
| CTTATTCGTGCCTTCAGCCC+TGG | 0.474395 | 4.3:-20955903 | None:intergenic |
| GATGGAAGCACACTTTGAAC+TGG | 0.475219 | 4.3:-20960172 | None:intergenic |
| TTGAAGCTGAAACGGTTGAG+TGG | 0.476160 | 4.3:+20962791 | MS.gene068015:CDS |
| CCGGAGTATAAAGGATGCTC+TGG | 0.477256 | 4.3:+20955459 | MS.gene068015:CDS |
| TCTTATTCTTGCTGTTACAC+CGG | 0.479476 | 4.3:+20954793 | MS.gene068015:CDS |
| ATGATCAGCCATTCCTCCAT+TGG | 0.480288 | 4.3:-20959693 | None:intergenic |
| CCCCTCCGACTTGGTTATGT+CGG | 0.488295 | 4.3:+20955162 | MS.gene068015:CDS |
| AGCGTAATGTCAAGAACATT+CGG | 0.491446 | 4.3:-20954665 | None:intergenic |
| GAATTCCGATGCTCTTCAAA+TGG | 0.491470 | 4.3:+20954832 | MS.gene068015:CDS |
| AGAAACACCTTTGTTCCCTC+CGG | 0.492151 | 4.3:-20954612 | None:intergenic |
| TCCAGCCATTTGAAGAGCAT+CGG | 0.495489 | 4.3:-20954837 | None:intergenic |
| GTCTCTGAACTTCTCTCTGA+TGG | 0.501227 | 4.3:-20960321 | None:intergenic |
| AGGTCCAGTTGCATTCTGTA+TGG | 0.505132 | 4.3:-20957260 | None:intergenic |
| GAGGGAGGTCGATGGTGGAT+TGG | 0.505265 | 4.3:-20954255 | None:intergenic |
| GAGATATCTGCCTCCGTACA+AGG | 0.505354 | 4.3:-20957445 | None:intergenic |
| TGTTGAGGATCTTATTCCTA+AGG | 0.508752 | 4.3:+20960410 | MS.gene068015:CDS |
| CCCTTTCTTGAGAAAACGCA+TGG | 0.509114 | 4.3:+20957651 | MS.gene068015:CDS |
| CTCCGTACAAGGACTTCAAA+CGG | 0.509243 | 4.3:-20957434 | None:intergenic |
| TATATATGACGAGTTAATGA+AGG | 0.509634 | 4.3:+20957502 | MS.gene068015:CDS |
| AACCTGGCACATTGAAGACT+TGG | 0.509646 | 4.3:-20957479 | None:intergenic |
| TTCCGTTTGAAGTCCTTGTA+CGG | 0.510952 | 4.3:+20957432 | MS.gene068015:CDS |
| GCTGAGTTTCTTCATTTACC+TGG | 0.511295 | 4.3:+20954430 | MS.gene068015:CDS |
| AGATTACGGTGGATCCTAGC+GGG | 0.512805 | 4.3:-20954189 | None:intergenic |
| CTTAGAAACTGTAGAAGACT+TGG | 0.514125 | 4.3:-20959476 | None:intergenic |
| CTCAAATGAATTGTAGAGGA+TGG | 0.514624 | 4.3:-20960190 | None:intergenic |
| TTGTACGGAGGCAGATATCT+CGG | 0.515018 | 4.3:+20957447 | MS.gene068015:CDS |
| TGTTCTTGACATTACGCTTG+TGG | 0.517699 | 4.3:+20954670 | MS.gene068015:CDS |
| TGAAGCTGAAACGGTTGAGT+GGG | 0.519060 | 4.3:+20962792 | MS.gene068015:CDS |
| TCCAACGACGGCGCCCCGCT+AGG | 0.520684 | 4.3:+20954175 | MS.gene068015:CDS |
| AAAGTTATTCCCCTCCGACT+TGG | 0.523941 | 4.3:+20955153 | MS.gene068015:CDS |
| TTCTACAGTTTCTAAGGTGA+AGG | 0.527882 | 4.3:+20959483 | MS.gene068015:CDS |
| ATCCACCATCGACCTCCCTC+AGG | 0.531578 | 4.3:+20954258 | MS.gene068015:CDS |
| GCTACTCTGGCTGCCGACGA+CGG | 0.532120 | 4.3:-20954284 | None:intergenic |
| GCAAAGGAACATGCAAGCTA+TGG | 0.532385 | 4.3:+20955945 | MS.gene068015:CDS |
| ACTACCTCTTGAAGCTGAAA+CGG | 0.534191 | 4.3:+20962783 | MS.gene068015:CDS |
| GTAGAATACATAGAAGAAGT+TGG | 0.534563 | 4.3:-20962862 | None:intergenic |
| CGGTGTTGTAAATCGTAGCC+AGG | 0.534919 | 4.3:+20955182 | MS.gene068015:CDS |
| GGAACAAACAATGCTGCTCT+AGG | 0.536557 | 4.3:-20957280 | None:intergenic |
| TGGGTGATCATTGTCTCTGA+GGG | 0.537368 | 4.3:-20957712 | None:intergenic |
| ACGGGAACCTTGGTAATACC+AGG | 0.537492 | 4.3:-20954698 | None:intergenic |
| TTCCACCAATAAAATTTGGG+TGG | 0.538529 | 4.3:-20959423 | None:intergenic |
| TTGATCACCAACGGGAACCT+TGG | 0.539530 | 4.3:-20954708 | None:intergenic |
| TCTGAAGGTTGATCACCAAC+GGG | 0.540101 | 4.3:-20954716 | None:intergenic |
| GGCTGTGCTACCAGGGCTGA+AGG | 0.540827 | 4.3:+20955893 | MS.gene068015:CDS |
| ACTGGCTTGCCTAAAATTCG+CGG | 0.540843 | 4.3:+20962835 | MS.gene068015:CDS |
| GCTGAGACTGATAGGGAAGC+CGG | 0.544041 | 4.3:+20954593 | MS.gene068015:CDS |
| TTTGTAGGCTGAGACTGATA+GGG | 0.547959 | 4.3:+20954586 | MS.gene068015:intron |
| TAAAAGCTTGTTGATAAGCT+CGG | 0.551504 | 4.3:-20961239 | None:intergenic |
| ATCTGAAGGTTGATCACCAA+CGG | 0.552721 | 4.3:-20954717 | None:intergenic |
| TGTATCCCCTCAGGAACCAC+TGG | 0.558758 | 4.3:+20960058 | MS.gene068015:intron |
| CTGCCGACGACGGCAACCTG+AGG | 0.559115 | 4.3:-20954274 | None:intergenic |
| AAATCATGGAAAGTGCGGCC+AGG | 0.560881 | 4.3:-20954448 | None:intergenic |
| CCCTAACCAAACTATGGCGG+CGG | 0.561738 | 4.3:+20954150 | None:intergenic |
| TCCACCAATAAAATTTGGGT+GGG | 0.563887 | 4.3:-20959422 | None:intergenic |
| TTCACTTGTCAATCTTGCAA+AGG | 0.564530 | 4.3:+20955929 | MS.gene068015:CDS |
| CCAGAGCATCCTTTATACTC+CGG | 0.564711 | 4.3:-20955459 | None:intergenic |
| GATTCTTGCACAACACATCA+AGG | 0.565933 | 4.3:+20955872 | MS.gene068015:intron |
| ATTCTTTCCCTCTACTATGG+AGG | 0.566704 | 4.3:-20956344 | None:intergenic |
| GGATCAGCTTCTGAAAGAAG+TGG | 0.571099 | 4.3:+20959641 | MS.gene068015:CDS |
| TGGAGAAATCACGGAGTCCA+AGG | 0.574229 | 4.3:+20955965 | MS.gene068015:CDS |
| ACAGCAAGAATAAGACAAGT+AGG | 0.575319 | 4.3:-20954785 | None:intergenic |
| TGTGCAGATATCGTTACCAC+GGG | 0.577032 | 4.3:-20954353 | None:intergenic |
| TGGACAGAGGTACCGATGCT+CGG | 0.577331 | 4.3:+20955115 | MS.gene068015:CDS |
| CACGGCCGACGAGAGCTTCG+AGG | 0.578474 | 4.3:-20954321 | None:intergenic |
| ATTCTTTCAAAATACTCTGA+CGG | 0.579502 | 4.3:+20956153 | MS.gene068015:CDS |
| AGATAACCGTGTGTCTGTGA+AGG | 0.581518 | 4.3:+20960120 | MS.gene068015:CDS |
| ATTACGCTTGTGGATCTCCC+TGG | 0.582531 | 4.3:+20954680 | MS.gene068015:CDS |
| AGGGAAGCCGGAGGGAACAA+AGG | 0.583252 | 4.3:+20954605 | MS.gene068015:CDS |
| TGGTGGATTGGCTTCCGACA+CGG | 0.583448 | 4.3:-20954243 | None:intergenic |
| GCTGATTCGGGTTTAGAAGG+TGG | 0.586580 | 4.3:-20954409 | None:intergenic |
| ATCCCCTCAGGAACCACTGG+TGG | 0.586660 | 4.3:+20960061 | MS.gene068015:intron |
| CGTTTGAAGTCCTTGTACGG+AGG | 0.587076 | 4.3:+20957435 | MS.gene068015:CDS |
| CACCCATATCATAGAAATGG+AGG | 0.587912 | 4.3:+20957729 | MS.gene068015:CDS |
| AGCATTCACCTCCATAGTAG+AGG | 0.588904 | 4.3:+20956336 | MS.gene068015:intron |
| CAAGACATCTTCAGCCGTGT+CGG | 0.591438 | 4.3:+20954229 | MS.gene068015:CDS |
| TGCCGACGACGGCAACCTGA+GGG | 0.591564 | 4.3:-20954273 | None:intergenic |
| ACCACTGGTGGATCTAGTTG+GGG | 0.591737 | 4.3:+20960073 | MS.gene068015:CDS |
| CATAGTAGAGGGAAAGAATG+AGG | 0.594573 | 4.3:+20956348 | MS.gene068015:CDS |
| CTGAGCTATCAGGTGGAGCA+CGG | 0.598034 | 4.3:+20956383 | MS.gene068015:CDS |
| ATGGGTGATCATTGTCTCTG+AGG | 0.604160 | 4.3:-20957713 | None:intergenic |
| CTCTACAATTCATTTGAGAG+AGG | 0.605023 | 4.3:+20960195 | MS.gene068015:CDS |
| CAGTAACAGATTCCGAGCAT+CGG | 0.605265 | 4.3:-20955127 | None:intergenic |
| GGTATTACCAAGGTTCCCGT+TGG | 0.605870 | 4.3:+20954701 | MS.gene068015:CDS |
| TTCGCCAAATCAGAATTAGC+CGG | 0.606020 | 4.3:-20954812 | None:intergenic |
| ATTCTCGATTCGATATCTGA+AGG | 0.606620 | 4.3:-20954731 | None:intergenic |
| GGATCTCCCTGGTATTACCA+AGG | 0.609003 | 4.3:+20954691 | MS.gene068015:CDS |
| GCATTCACCTCCATAGTAGA+GGG | 0.609702 | 4.3:+20956337 | MS.gene068015:intron |
| CTCTCTCAAATGAATTGTAG+AGG | 0.611486 | 4.3:-20960194 | None:intergenic |
| TGCAAGCTATGGAGAAATCA+CGG | 0.611972 | 4.3:+20955956 | MS.gene068015:CDS |
| CTTGCAAGACATGCCAATGG+AGG | 0.614495 | 4.3:+20959680 | MS.gene068015:CDS |
| GATGCTCGGAATCTGTTACT+GGG | 0.618289 | 4.3:+20955129 | MS.gene068015:CDS |
| CACCGACATAACCAAGTCGG+AGG | 0.623102 | 4.3:-20955164 | None:intergenic |
| ACATCAAGGCTGTGCTACCA+GGG | 0.623492 | 4.3:+20955886 | MS.gene068015:CDS |
| AAGACATGCCAATGGAGGAA+TGG | 0.625106 | 4.3:+20959685 | MS.gene068015:CDS |
| GGCGGTACGAATATCATCAT+CGG | 0.625270 | 4.3:-20957239 | None:intergenic |
| AACCTGAGGGAGGTCGATGG+TGG | 0.626380 | 4.3:-20954260 | None:intergenic |
| GTCGTCGGCAGCCAGAGTAG+CGG | 0.628211 | 4.3:+20954286 | MS.gene068015:CDS |
| AGCGTCCTCGAAGCTCTCGT+CGG | 0.629417 | 4.3:+20954316 | MS.gene068015:CDS |
| TCCAGTTGCATTCTGTATGG+CGG | 0.635952 | 4.3:-20957257 | None:intergenic |
| GGCAACCTGAGGGAGGTCGA+TGG | 0.638647 | 4.3:-20954263 | None:intergenic |
| ATGGCGGCGGTGTCCAACGA+CGG | 0.639282 | 4.3:+20954163 | MS.gene068015:CDS |
| TCAACCGTTTCAGCTTCAAG+AGG | 0.640027 | 4.3:-20962787 | None:intergenic |
| ATTCTTGCAAGACATGCCAA+TGG | 0.641227 | 4.3:+20959677 | MS.gene068015:CDS |
| GTGTGCAGATATCGTTACCA+CGG | 0.646207 | 4.3:-20954354 | None:intergenic |
| TGTGAACGAGTTGAAGAACA+AGG | 0.647322 | 4.3:-20954384 | None:intergenic |
| AACTATAGGTGTCATAACCA+AGG | 0.647581 | 4.3:+20954979 | MS.gene068015:CDS |
| GAGACTGATAGGGAAGCCGG+AGG | 0.649242 | 4.3:+20954596 | MS.gene068015:CDS |
| GGGAGAAATCATGGAAAGTG+CGG | 0.651182 | 4.3:-20954453 | None:intergenic |
| TAATTTCCAATAGGATGCTG+TGG | 0.652101 | 4.3:+20959607 | MS.gene068015:intron |
| ACCGACATAACCAAGTCGGA+GGG | 0.652428 | 4.3:-20955163 | None:intergenic |
| TAACTCGTCATATATAAACC+TGG | 0.654748 | 4.3:-20957495 | None:intergenic |
| AGTGGTCTAGCTGATAGTTG+TGG | 0.655528 | 4.3:+20955602 | MS.gene068015:CDS |
| TGATGGCCTCAAGATAGTAG+GGG | 0.656712 | 4.3:-20960304 | None:intergenic |
| TGAACGAGTTGAAGAACAAG+GGG | 0.657953 | 4.3:-20954382 | None:intergenic |
| GTTGACTAGTGAGATTACGG+TGG | 0.659709 | 4.3:-20954200 | None:intergenic |
| TATTCTCCTTCACAGACACA+CGG | 0.662699 | 4.3:-20960126 | None:intergenic |
| GATGGCCTCAAGATAGTAGG+GGG | 0.667216 | 4.3:-20960303 | None:intergenic |
| CGACGACGGCAACCTGAGGG+AGG | 0.670636 | 4.3:-20954270 | None:intergenic |
| GAAGCTGAAACGGTTGAGTG+GGG | 0.671223 | 4.3:+20962793 | MS.gene068015:CDS |
| TCATCGGTCAAGTCTTCACA+CGG | 0.672633 | 4.3:-20957223 | None:intergenic |
| TCTACATCTGAGCTATCAGG+TGG | 0.673530 | 4.3:+20956376 | MS.gene068015:CDS |
| GCTGGAAATGCTGATCCCGA+TGG | 0.678588 | 4.3:+20954854 | MS.gene068015:CDS |
| ATTGGCAAAGAAGTTGAACA+AGG | 0.682461 | 4.3:+20955634 | MS.gene068015:CDS |
| CAACACCGACATAACCAAGT+CGG | 0.684240 | 4.3:-20955167 | None:intergenic |
| GTGAACGAGTTGAAGAACAA+GGG | 0.684986 | 4.3:-20954383 | None:intergenic |
| AGACTGATAGGGAAGCCGGA+GGG | 0.696803 | 4.3:+20954597 | MS.gene068015:CDS |
| CGGGAACCTTGGTAATACCA+GGG | 0.698106 | 4.3:-20954697 | None:intergenic |
| TAGTTGGATATCATGGACAG+AGG | 0.713407 | 4.3:+20955102 | MS.gene068015:intron |
| CCGACATAACCAAGTCGGAG+GGG | 0.714844 | 4.3:-20955162 | None:intergenic |
| GAGATTACGGTGGATCCTAG+CGG | 0.715884 | 4.3:-20954190 | None:intergenic |
| GTTCAGTCACCATACAACGA+TGG | 0.718083 | 4.3:-20957618 | None:intergenic |
| CCACTGGTGGATCTAGTTGG+GGG | 0.719711 | 4.3:+20960074 | MS.gene068015:CDS |
| TGCAGATATCGTTACCACGG+GGG | 0.724538 | 4.3:-20954351 | None:intergenic |
| GATTACGGTGGATCCTAGCG+GGG | 0.732843 | 4.3:-20954188 | None:intergenic |
| TGTTGTAAATCGTAGCCAGG+AGG | 0.732912 | 4.3:+20955185 | MS.gene068015:CDS |
| ACTTCCTTACAGGATGACTG+CGG | 0.758275 | 4.3:-20955501 | None:intergenic |
| AAGGAACCTGAATTTCAGCG+CGG | 0.764271 | 4.3:-20954480 | None:intergenic |
| GTGCAGATATCGTTACCACG+GGG | 0.769026 | 4.3:-20954352 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 20954163 | 20962909 | 20954163 | ID=MS.gene068015 |
| chr4.3 | mRNA | 20954163 | 20962909 | 20954163 | ID=MS.gene068015.t1;Parent=MS.gene068015 |
| chr4.3 | exon | 20954163 | 20954495 | 20954163 | ID=MS.gene068015.t1.exon1;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20954163 | 20954495 | 20954163 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20954593 | 20954875 | 20954593 | ID=MS.gene068015.t1.exon2;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20954593 | 20954875 | 20954593 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20954972 | 20955000 | 20954972 | ID=MS.gene068015.t1.exon3;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20954972 | 20955000 | 20954972 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20955105 | 20955206 | 20955105 | ID=MS.gene068015.t1.exon4;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20955105 | 20955206 | 20955105 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20955445 | 20955513 | 20955445 | ID=MS.gene068015.t1.exon5;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20955445 | 20955513 | 20955445 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20955596 | 20955655 | 20955596 | ID=MS.gene068015.t1.exon6;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20955596 | 20955655 | 20955596 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20955873 | 20955986 | 20955873 | ID=MS.gene068015.t1.exon7;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20955873 | 20955986 | 20955873 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20956126 | 20956174 | 20956126 | ID=MS.gene068015.t1.exon8;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20956126 | 20956174 | 20956126 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20956338 | 20956447 | 20956338 | ID=MS.gene068015.t1.exon9;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20956338 | 20956447 | 20956338 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20957214 | 20957306 | 20957214 | ID=MS.gene068015.t1.exon10;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20957214 | 20957306 | 20957214 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20957431 | 20957523 | 20957431 | ID=MS.gene068015.t1.exon11;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20957431 | 20957523 | 20957431 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20957613 | 20957750 | 20957613 | ID=MS.gene068015.t1.exon12;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20957613 | 20957750 | 20957613 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20959403 | 20959504 | 20959403 | ID=MS.gene068015.t1.exon13;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20959403 | 20959504 | 20959403 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20959620 | 20959715 | 20959620 | ID=MS.gene068015.t1.exon14;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20959620 | 20959715 | 20959620 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20959856 | 20959898 | 20959856 | ID=MS.gene068015.t1.exon15;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20959856 | 20959898 | 20959856 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20960071 | 20960216 | 20960071 | ID=MS.gene068015.t1.exon16;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20960071 | 20960216 | 20960071 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20960303 | 20960449 | 20960303 | ID=MS.gene068015.t1.exon17;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20960303 | 20960449 | 20960303 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20961016 | 20961068 | 20961016 | ID=MS.gene068015.t1.exon18;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20961016 | 20961068 | 20961016 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20961164 | 20961263 | 20961164 | ID=MS.gene068015.t1.exon19;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20961164 | 20961263 | 20961164 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| chr4.3 | exon | 20962772 | 20962909 | 20962772 | ID=MS.gene068015.t1.exon20;Parent=MS.gene068015.t1 |
| chr4.3 | CDS | 20962772 | 20962909 | 20962772 | ID=cds.MS.gene068015.t1;Parent=MS.gene068015.t1 |
| Gene Sequence |
| Protein sequence |
