Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39665.t1 | XP_003626828.1 | 98.4 | 637 | 10 | 0 | 1 | 637 | 1 | 637 | 0.00E+00 | 1195.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39665.t1 | Q9FNX5 | 77.7 | 637 | 129 | 1 | 1 | 637 | 1 | 624 | 1.3e-292 | 1006.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39665.t1 | G7LDH0 | 98.4 | 637 | 10 | 0 | 1 | 637 | 1 | 637 | 0.0e+00 | 1195.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene39665 | 0.849871 | 2.45E-60 | -1.69E-46 |
| MS.gene049983 | MS.gene39665 | 0.816515 | 5.32E-52 | -1.69E-46 |
| MS.gene050105 | MS.gene39665 | 0.804535 | 2.06E-49 | -1.69E-46 |
| MS.gene050571 | MS.gene39665 | 0.800526 | 1.38E-48 | -1.69E-46 |
| MS.gene050654 | MS.gene39665 | 0.829211 | 5.84E-55 | -1.69E-46 |
| MS.gene051122 | MS.gene39665 | 0.841072 | 5.93E-58 | -1.69E-46 |
| MS.gene051541 | MS.gene39665 | 0.819553 | 1.09E-52 | -1.69E-46 |
| MS.gene052118 | MS.gene39665 | 0.810529 | 1.10E-50 | -1.69E-46 |
| MS.gene05216 | MS.gene39665 | 0.807293 | 5.43E-50 | -1.69E-46 |
| MS.gene052493 | MS.gene39665 | 0.805933 | 1.05E-49 | -1.69E-46 |
| MS.gene052674 | MS.gene39665 | 0.816773 | 4.65E-52 | -1.69E-46 |
| MS.gene052675 | MS.gene39665 | 0.816396 | 5.65E-52 | -1.69E-46 |
| MS.gene052677 | MS.gene39665 | 0.817928 | 2.56E-52 | -1.69E-46 |
| MS.gene053726 | MS.gene39665 | 0.821956 | 3.06E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene39665 | 0.821701 | 3.51E-53 | -1.69E-46 |
| MS.gene05439 | MS.gene39665 | 0.839572 | 1.46E-57 | -1.69E-46 |
| MS.gene054479 | MS.gene39665 | 0.840409 | 8.85E-58 | -1.69E-46 |
| MS.gene054892 | MS.gene39665 | 0.851213 | 1.03E-60 | -1.69E-46 |
| MS.gene054893 | MS.gene39665 | 0.853024 | 3.14E-61 | -1.69E-46 |
| MS.gene055327 | MS.gene39665 | 0.806045 | 9.95E-50 | -1.69E-46 |
| MS.gene056849 | MS.gene39665 | 0.81541 | 9.38E-52 | -1.69E-46 |
| MS.gene056973 | MS.gene39665 | 0.826512 | 2.60E-54 | -1.69E-46 |
| MS.gene057155 | MS.gene39665 | 0.81382 | 2.11E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene39665 | 0.805137 | 1.54E-49 | -1.69E-46 |
| MS.gene058223 | MS.gene39665 | -0.82423 | 9.03E-54 | -1.69E-46 |
| MS.gene058289 | MS.gene39665 | 0.80883 | 2.55E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene39665 | 0.843367 | 1.46E-58 | -1.69E-46 |
| MS.gene060259 | MS.gene39665 | 0.805778 | 1.13E-49 | -1.69E-46 |
| MS.gene060412 | MS.gene39665 | 0.827245 | 1.74E-54 | -1.69E-46 |
| MS.gene060413 | MS.gene39665 | 0.822856 | 1.89E-53 | -1.69E-46 |
| MS.gene060520 | MS.gene39665 | 0.830018 | 3.72E-55 | -1.69E-46 |
| MS.gene060840 | MS.gene39665 | 0.80521 | 1.49E-49 | -1.69E-46 |
| MS.gene060850 | MS.gene39665 | 0.848258 | 6.87E-60 | -1.69E-46 |
| MS.gene060851 | MS.gene39665 | 0.870266 | 1.66E-66 | -1.69E-46 |
| MS.gene060852 | MS.gene39665 | 0.866593 | 2.55E-65 | -1.69E-46 |
| MS.gene060932 | MS.gene39665 | 0.81824 | 2.17E-52 | -1.69E-46 |
| MS.gene06121 | MS.gene39665 | 0.814267 | 1.68E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene39665 | MS.gene06079 | PPI |
| MS.gene39665 | MS.gene068015 | PPI |
| MS.gene39665 | MS.gene90230 | PPI |
| MS.gene39665 | MS.gene053588 | PPI |
| MS.gene39665 | MS.gene069595 | PPI |
| MS.gene39665 | MS.gene048935 | PPI |
| MS.gene39665 | MS.gene55806 | PPI |
| MS.gene39665 | MS.gene073953 | PPI |
| MS.gene39665 | MS.gene22300 | PPI |
| MS.gene39665 | MS.gene53578 | PPI |
| MS.gene39665 | MS.gene064936 | PPI |
| MS.gene39665 | MS.gene21671 | PPI |
| MS.gene39665 | MS.gene002248 | PPI |
| MS.gene39665 | MS.gene64242 | PPI |
| MS.gene39665 | MS.gene028493 | PPI |
| MS.gene39665 | MS.gene01221 | PPI |
| MS.gene39665 | MS.gene02205 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39665.t1 | MTR_8g009510 | 98.430 | 637 | 10 | 0 | 1 | 637 | 1 | 637 | 0.0 | 1295 |
| MS.gene39665.t1 | MTR_7g451010 | 84.301 | 637 | 80 | 1 | 1 | 637 | 1 | 617 | 0.0 | 1077 |
| MS.gene39665.t1 | MTR_8g009510 | 98.302 | 530 | 9 | 0 | 108 | 637 | 1 | 530 | 0.0 | 1077 |
| MS.gene39665.t1 | MTR_8g105580 | 76.063 | 635 | 132 | 3 | 1 | 635 | 1 | 615 | 0.0 | 1004 |
| MS.gene39665.t1 | MTR_4g098920 | 72.310 | 632 | 148 | 2 | 4 | 635 | 1 | 605 | 0.0 | 971 |
| MS.gene39665.t1 | MTR_1g032550 | 72.913 | 635 | 151 | 2 | 1 | 635 | 1 | 614 | 0.0 | 971 |
| MS.gene39665.t1 | MTR_8g105580 | 76.975 | 595 | 117 | 3 | 1 | 595 | 1 | 575 | 0.0 | 949 |
| MS.gene39665.t1 | MTR_8g099550 | 65.823 | 632 | 192 | 2 | 4 | 635 | 1 | 608 | 0.0 | 897 |
| MS.gene39665.t1 | MTR_5g009150 | 65.142 | 634 | 198 | 2 | 4 | 637 | 1 | 611 | 0.0 | 889 |
| MS.gene39665.t1 | MTR_8g027675 | 65.665 | 632 | 192 | 3 | 4 | 635 | 1 | 607 | 0.0 | 880 |
| MS.gene39665.t1 | MTR_2g009690 | 38.306 | 496 | 282 | 5 | 7 | 493 | 24 | 504 | 3.12e-97 | 316 |
| MS.gene39665.t1 | MTR_4g093750 | 38.431 | 497 | 282 | 5 | 6 | 493 | 14 | 495 | 2.18e-96 | 311 |
| MS.gene39665.t1 | MTR_4g093750 | 38.353 | 498 | 283 | 5 | 6 | 493 | 14 | 497 | 1.82e-95 | 310 |
| MS.gene39665.t1 | MTR_4g134680 | 30.502 | 518 | 323 | 11 | 1 | 501 | 1 | 498 | 8.05e-68 | 236 |
| MS.gene39665.t1 | MTR_4g134680 | 30.502 | 518 | 323 | 11 | 1 | 501 | 1 | 498 | 2.02e-67 | 236 |
| MS.gene39665.t1 | MTR_4g030140 | 30.754 | 517 | 326 | 9 | 1 | 501 | 1 | 501 | 6.68e-67 | 236 |
| MS.gene39665.t1 | MTR_4g134680 | 30.502 | 518 | 323 | 11 | 1 | 501 | 1 | 498 | 7.14e-67 | 235 |
| MS.gene39665.t1 | MTR_4g134680 | 38.806 | 201 | 123 | 0 | 301 | 501 | 1 | 201 | 2.54e-40 | 156 |
| MS.gene39665.t1 | MTR_8g087750 | 34.494 | 316 | 183 | 9 | 40 | 346 | 71 | 371 | 2.13e-31 | 130 |
| MS.gene39665.t1 | MTR_8g087770 | 34.906 | 318 | 179 | 10 | 40 | 346 | 68 | 368 | 3.47e-31 | 129 |
| MS.gene39665.t1 | MTR_8g463270 | 33.161 | 193 | 110 | 4 | 40 | 220 | 46 | 231 | 9.40e-19 | 91.3 |
| MS.gene39665.t1 | MTR_4g094300 | 27.807 | 187 | 127 | 4 | 41 | 220 | 41 | 226 | 7.34e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39665.t1 | AT3G60190 | 78.179 | 637 | 126 | 2 | 1 | 637 | 1 | 624 | 0.0 | 1057 |
| MS.gene39665.t1 | AT1G14830 | 75.276 | 635 | 135 | 2 | 1 | 635 | 1 | 613 | 0.0 | 999 |
| MS.gene39665.t1 | AT2G44590 | 71.767 | 634 | 157 | 3 | 4 | 637 | 1 | 612 | 0.0 | 957 |
| MS.gene39665.t1 | AT2G44590 | 69.085 | 634 | 157 | 4 | 4 | 637 | 1 | 595 | 0.0 | 914 |
| MS.gene39665.t1 | AT2G44590 | 68.976 | 635 | 157 | 5 | 4 | 637 | 1 | 596 | 0.0 | 909 |
| MS.gene39665.t1 | AT5G42080 | 65.665 | 632 | 193 | 2 | 4 | 635 | 1 | 608 | 0.0 | 889 |
| MS.gene39665.t1 | AT5G42080 | 65.665 | 632 | 193 | 2 | 4 | 635 | 1 | 608 | 0.0 | 889 |
| MS.gene39665.t1 | AT3G61760 | 64.929 | 633 | 196 | 4 | 4 | 635 | 1 | 608 | 0.0 | 878 |
| MS.gene39665.t1 | AT5G42080 | 65.032 | 632 | 191 | 3 | 4 | 635 | 1 | 602 | 0.0 | 873 |
| MS.gene39665.t1 | AT3G61760 | 65.936 | 593 | 176 | 4 | 4 | 595 | 1 | 568 | 0.0 | 833 |
| MS.gene39665.t1 | AT3G61760 | 71.652 | 448 | 122 | 3 | 4 | 450 | 1 | 444 | 0.0 | 690 |
| MS.gene39665.t1 | AT5G42080 | 72.917 | 432 | 114 | 1 | 4 | 435 | 1 | 429 | 0.0 | 681 |
| MS.gene39665.t1 | AT2G14120 | 38.569 | 503 | 279 | 8 | 6 | 493 | 20 | 507 | 5.57e-98 | 316 |
| MS.gene39665.t1 | AT2G14120 | 38.569 | 503 | 279 | 8 | 6 | 493 | 20 | 507 | 1.78e-97 | 316 |
| MS.gene39665.t1 | AT2G14120 | 38.569 | 503 | 279 | 8 | 6 | 493 | 20 | 507 | 1.83e-97 | 316 |
| MS.gene39665.t1 | AT2G14120 | 36.654 | 532 | 278 | 9 | 6 | 493 | 20 | 536 | 2.84e-93 | 305 |
| MS.gene39665.t1 | AT4G33650 | 37.052 | 502 | 287 | 7 | 6 | 493 | 36 | 522 | 1.83e-89 | 295 |
| MS.gene39665.t1 | AT4G33650 | 37.052 | 502 | 287 | 7 | 6 | 493 | 36 | 522 | 2.29e-89 | 295 |
| MS.gene39665.t1 | AT1G10290 | 32.108 | 517 | 316 | 10 | 1 | 501 | 1 | 498 | 2.06e-67 | 237 |
| MS.gene39665.t1 | AT1G59610 | 32.370 | 519 | 312 | 14 | 1 | 501 | 1 | 498 | 8.95e-67 | 235 |
| MS.gene39665.t1 | AT1G60530 | 42.857 | 203 | 108 | 4 | 40 | 237 | 62 | 261 | 4.21e-37 | 140 |
| MS.gene39665.t1 | AT1G60500 | 34.528 | 307 | 188 | 7 | 40 | 344 | 65 | 360 | 3.92e-36 | 144 |
| MS.gene39665.t1 | AT1G53140 | 33.161 | 193 | 110 | 4 | 40 | 220 | 62 | 247 | 2.28e-19 | 93.2 |
Find 179 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTACTAATCCATCTAAT+TGG | 0.176287 | 8.3:-79540836 | MS.gene39665:CDS |
| GGAAGGAAGATTACCGATTT+TGG | 0.210097 | 8.3:-79546173 | MS.gene39665:intron |
| ACAATACTTTCTCTTCTCTA+TGG | 0.226867 | 8.3:-79547154 | MS.gene39665:CDS |
| TGTTCAAGATATTGAAAATA+TGG | 0.234209 | 8.3:-79543768 | MS.gene39665:CDS |
| AACTATTCGGCTTGTGGATA+TGG | 0.268455 | 8.3:-79540931 | MS.gene39665:CDS |
| AGCACTTGGAGTCGGTAATT+AGG | 0.272153 | 8.3:-79542262 | MS.gene39665:CDS |
| ATGAAATTGATTCGGTTTCT+TGG | 0.284411 | 8.3:-79539761 | MS.gene39665:CDS |
| CAGTTTCGTCTTGAATTTCC+TGG | 0.285024 | 8.3:+79545557 | None:intergenic |
| TGGCCACTTAGCCAGTAAAA+TGG | 0.287222 | 8.3:-79542387 | MS.gene39665:CDS |
| CCTAACTTTGATTGATTTGC+CGG | 0.293875 | 8.3:-79543941 | MS.gene39665:CDS |
| TTCAGGGAAATCTTCGGTTT+TGG | 0.304850 | 8.3:-79546989 | MS.gene39665:CDS |
| GACTACTACTTTACCAAAAT+CGG | 0.316139 | 8.3:+79546160 | None:intergenic |
| CTGATGCTATCAAAATTTCC+AGG | 0.329530 | 8.3:-79542796 | MS.gene39665:CDS |
| TGGAGATGAGAACAGAGTTC+AGG | 0.339919 | 8.3:-79547006 | MS.gene39665:intron |
| TCTGGATTACCCCAATTAGA+TGG | 0.345068 | 8.3:+79540825 | None:intergenic |
| ACCTACTAATCCATCTAATT+GGG | 0.355183 | 8.3:-79540835 | MS.gene39665:CDS |
| GAATAAATGCTAAGATGAAT+TGG | 0.360365 | 8.3:+79545496 | None:intergenic |
| AGTTGTAGCTGCAGCTAATT+CGG | 0.378066 | 8.3:+79540992 | None:intergenic |
| GCACTTGGAGTCGGTAATTA+GGG | 0.378515 | 8.3:-79542261 | MS.gene39665:CDS |
| CGAGTTTAGTACAAGCTTGT+TGG | 0.379634 | 8.3:+79547201 | None:intergenic |
| CTTCTTCGTGATGACGACTA+TGG | 0.382344 | 8.3:-79547252 | None:intergenic |
| AGTCTCCGGTAACCTTGCTC+TGG | 0.390633 | 8.3:+79541328 | None:intergenic |
| TGAAATTGATTCGGTTTCTT+GGG | 0.392405 | 8.3:-79539760 | MS.gene39665:CDS |
| GATGGATTAGTAGGTTGATC+TGG | 0.393618 | 8.3:+79540807 | None:intergenic |
| GGGACTCTCAGTTACTTTAG+AGG | 0.394944 | 8.3:-79541298 | MS.gene39665:CDS |
| GACGAAACTGATAGAATTAC+GGG | 0.396420 | 8.3:-79545544 | MS.gene39665:CDS |
| ATAGCCAGTATGATACAATT+AGG | 0.396892 | 8.3:+79542848 | None:intergenic |
| CCCCAATTAGATGGATTAGT+AGG | 0.398285 | 8.3:+79540834 | None:intergenic |
| TTTGAAACCAGTCCTGACTA+TGG | 0.400319 | 8.3:-79542407 | MS.gene39665:CDS |
| TGACAATAAACCACAGCCTT+TGG | 0.401522 | 8.3:+79540672 | None:intergenic |
| GCAGCCTAATTGTATCATAC+TGG | 0.404081 | 8.3:-79542852 | MS.gene39665:intron |
| CCATTTCTTGAGGAAGTCTT+CGG | 0.405046 | 8.3:+79540880 | None:intergenic |
| GACGATAAGATCTCCCTTCA+AGG | 0.408055 | 8.3:+79542525 | None:intergenic |
| AAAGGCTGTGGTTTATTGTC+AGG | 0.408788 | 8.3:-79540670 | MS.gene39665:CDS |
| CCGAAGACTTCCTCAAGAAA+TGG | 0.408850 | 8.3:-79540880 | MS.gene39665:CDS |
| ATGCTAAGATGAATTGGAAT+AGG | 0.409960 | 8.3:+79545502 | None:intergenic |
| AGACGAAACTGATAGAATTA+CGG | 0.412288 | 8.3:-79545545 | MS.gene39665:CDS |
| TGTACTAAACTCGGTGACTA+TGG | 0.413767 | 8.3:-79547191 | MS.gene39665:CDS |
| CAAATTTGCGGCAAAGTTCA+AGG | 0.417106 | 8.3:+79542030 | None:intergenic |
| AAATCGGTAATCTTCCTTCC+TGG | 0.419121 | 8.3:+79546176 | None:intergenic |
| GCTGACAAAGCTGGATTTGA+TGG | 0.421276 | 8.3:-79542651 | MS.gene39665:CDS |
| TTGCCCCAGAGCAAGGTTAC+CGG | 0.423029 | 8.3:-79541333 | MS.gene39665:CDS |
| GTATTCAAAGAGCATTTAGA+TGG | 0.423316 | 8.3:-79542002 | MS.gene39665:CDS |
| ACGACTATGGAAAGCTTAAT+CGG | 0.423389 | 8.3:-79547239 | MS.gene39665:CDS |
| CTTAGCATTTATTCTCCAAA+TGG | 0.424920 | 8.3:-79545487 | MS.gene39665:intron |
| CTGCAGCTAATTCGGCTTGA+AGG | 0.425382 | 8.3:+79541000 | None:intergenic |
| TTTATGCCAGCACGGTTAGA+TGG | 0.427096 | 8.3:+79540789 | None:intergenic |
| TTATCGTCTGCAACATCCTT+GGG | 0.428575 | 8.3:-79542510 | MS.gene39665:CDS |
| ACCTGAGGGGTCCACTTCCC+TGG | 0.428644 | 8.3:+79542778 | None:intergenic |
| TGAATACACGTTCAAATTTG+CGG | 0.432203 | 8.3:+79542018 | None:intergenic |
| CCGGCAAATCAATCAAAGTT+AGG | 0.433564 | 8.3:+79543941 | None:intergenic |
| GGAGATGAGAACAGAGTTCA+GGG | 0.433664 | 8.3:-79547005 | MS.gene39665:intron |
| TACTGCATCAACTGAAGCTT+CGG | 0.433786 | 8.3:+79541270 | None:intergenic |
| TGTCCAGGTGAGAGGACATT+TGG | 0.439687 | 8.3:-79542677 | MS.gene39665:intron |
| CGTCGCTGTCGTCGGTGGCC+AGG | 0.441580 | 8.3:-79547117 | MS.gene39665:intron |
| AGACCAATTGCTGTTGATGC+GGG | 0.446141 | 8.3:-79542164 | MS.gene39665:CDS |
| AGCAAGGGATGAAATTGATT+CGG | 0.449058 | 8.3:-79539769 | MS.gene39665:CDS |
| TTTATCAAAGATGTTATACT+AGG | 0.449754 | 8.3:+79542230 | None:intergenic |
| GTCTCCGGTAACCTTGCTCT+GGG | 0.449780 | 8.3:+79541329 | None:intergenic |
| GAAGACAACAATGTTTCAAA+AGG | 0.451865 | 8.3:-79539809 | MS.gene39665:CDS |
| CTTATCGTCTGCAACATCCT+TGG | 0.452858 | 8.3:-79542511 | MS.gene39665:CDS |
| GTTGCTATGTCCTGGTTGGC+TGG | 0.453996 | 8.3:+79542821 | None:intergenic |
| CAATACTTTCTCTTCTCTAT+GGG | 0.459693 | 8.3:-79547153 | MS.gene39665:CDS |
| GCACGGTTAGATGGATTAGT+AGG | 0.466688 | 8.3:+79540798 | None:intergenic |
| ATTTGGCGTGCTGACAAAGC+TGG | 0.467319 | 8.3:-79542660 | MS.gene39665:CDS |
| ACAGGCGACCAGGAGGAGAT+AGG | 0.469255 | 8.3:-79541480 | MS.gene39665:intron |
| CTGGCTAAGTGGCCATAGTC+AGG | 0.469474 | 8.3:+79542395 | None:intergenic |
| AATCAGAGATGGATCATCTT+GGG | 0.469804 | 8.3:-79542187 | MS.gene39665:CDS |
| GACTTTCTACCTAGAGGATC+AGG | 0.470180 | 8.3:-79546949 | MS.gene39665:intron |
| GCATCAACTGAAGCTTCGGC+TGG | 0.470576 | 8.3:+79541274 | None:intergenic |
| GATGGATTAGTAGGTTGAGC+TGG | 0.471722 | 8.3:+79540843 | None:intergenic |
| GAATCAGAGATGGATCATCT+TGG | 0.476439 | 8.3:-79542188 | MS.gene39665:CDS |
| GAACTTGTGAGAAAATCAAT+AGG | 0.478284 | 8.3:-79541139 | MS.gene39665:CDS |
| TTATGCAGCGATGGTACGCC+AGG | 0.479699 | 8.3:-79545575 | MS.gene39665:intron |
| CAACTTCATAAGATAGAAGA+AGG | 0.481307 | 8.3:-79546230 | MS.gene39665:CDS |
| GGCCACTTAGCCAGTAAAAT+GGG | 0.483415 | 8.3:-79542386 | MS.gene39665:CDS |
| ATACTCAACCTCCTTGCGCC+TGG | 0.484858 | 8.3:+79542430 | None:intergenic |
| TGTCTTCTCTCCATCACGGT+AGG | 0.486322 | 8.3:+79539825 | None:intergenic |
| TACGTAAAGCAGCAGGAAGC+TGG | 0.489120 | 8.3:+79541437 | None:intergenic |
| ACAGAGTTCAGGGAAATCTT+CGG | 0.489500 | 8.3:-79546995 | MS.gene39665:intron |
| AGAGAAAGATGTTTGTCAAT+CGG | 0.497168 | 8.3:+79541409 | None:intergenic |
| AACTATTTCTACACACAGAT+AGG | 0.498011 | 8.3:-79540621 | MS.gene39665:CDS |
| AGAGTAAGAAGACAACTATT+CGG | 0.503106 | 8.3:-79540944 | MS.gene39665:CDS |
| CACTTAATTGCCCCAGAGCA+AGG | 0.512092 | 8.3:-79541340 | MS.gene39665:CDS |
| GGCTATATCTCCAGCCAACC+AGG | 0.515519 | 8.3:-79542831 | MS.gene39665:CDS |
| CTGGGGCAATTAAGTGAGGC+TGG | 0.517726 | 8.3:+79541347 | None:intergenic |
| TATGGAAAGCTTAATCGGCT+TGG | 0.517895 | 8.3:-79547234 | MS.gene39665:CDS |
| CTTCCTTCCGTCGCTGTCGT+CGG | 0.520946 | 8.3:-79547125 | MS.gene39665:CDS |
| GTTTATTGTCACAGGCGACC+AGG | 0.522732 | 8.3:-79541490 | MS.gene39665:intron |
| TGCAGCTAATTCGGCTTGAA+GGG | 0.525361 | 8.3:+79541001 | None:intergenic |
| GATTGTGACGAGGCGTCCAT+TGG | 0.525514 | 8.3:-79546258 | MS.gene39665:CDS |
| AATGACGATCGTTACGGTGA+GGG | 0.526248 | 8.3:-79540768 | MS.gene39665:CDS |
| GCTAATTCGGCTTGAAGGGT+AGG | 0.528310 | 8.3:+79541005 | None:intergenic |
| CATCAACTGAAGCTTCGGCT+GGG | 0.528711 | 8.3:+79541275 | None:intergenic |
| GGCATAAATGACGATCGTTA+CGG | 0.532941 | 8.3:-79540774 | MS.gene39665:CDS |
| TCCAGGGAAGTGGACCCCTC+AGG | 0.537660 | 8.3:-79542779 | MS.gene39665:intron |
| GAATGGTGTCGGACACACTC+AGG | 0.542112 | 8.3:-79540704 | MS.gene39665:CDS |
| CATCAGAAGTTGCTATGTCC+TGG | 0.543250 | 8.3:+79542813 | None:intergenic |
| GTCTTCTCTCCATCACGGTA+GGG | 0.543606 | 8.3:+79539826 | None:intergenic |
| GAAGACAACTATTCGGCTTG+TGG | 0.544489 | 8.3:-79540937 | MS.gene39665:CDS |
| TGTGAAGAGAGTGGTGTCAG+AGG | 0.544625 | 8.3:-79541380 | MS.gene39665:CDS |
| GAGACCAATTGCTGTTGATG+CGG | 0.545777 | 8.3:-79542165 | MS.gene39665:CDS |
| GAAAGAGAACCTGATCCTCT+AGG | 0.546071 | 8.3:+79546940 | None:intergenic |
| CGAAGAACTTGAATCAGAGA+TGG | 0.546993 | 8.3:-79542198 | MS.gene39665:CDS |
| GACCAATTGCTGTTGATGCG+GGG | 0.549430 | 8.3:-79542163 | MS.gene39665:CDS |
| ATGACGATCGTTACGGTGAG+GGG | 0.551047 | 8.3:-79540767 | MS.gene39665:CDS |
| TTATATATCCTATCTCCTCC+TGG | 0.551940 | 8.3:+79541472 | None:intergenic |
| CCTTGCAAAACTGCTCTCAC+AGG | 0.554182 | 8.3:-79542354 | MS.gene39665:intron |
| CCAGCTTTCTCCATTTCTTG+AGG | 0.555810 | 8.3:+79540870 | None:intergenic |
| AGAGAAAGTATTGTTGCTAT+CGG | 0.555953 | 8.3:+79547163 | None:intergenic |
| CAACAAGCTTGTACTAAACT+CGG | 0.559054 | 8.3:-79547200 | MS.gene39665:CDS |
| CTAATTCGGCTTGAAGGGTA+GGG | 0.560321 | 8.3:+79541006 | None:intergenic |
| CTAACTTTGATTGATTTGCC+GGG | 0.561184 | 8.3:-79543940 | MS.gene39665:CDS |
| TTCAAAGAGCATTTAGATGG+AGG | 0.561465 | 8.3:-79541999 | MS.gene39665:CDS |
| TATCAAAATTTCCAGGGAAG+TGG | 0.563515 | 8.3:-79542789 | MS.gene39665:CDS |
| GGTCACTATTCCAAAGGCTG+TGG | 0.564280 | 8.3:-79540682 | MS.gene39665:CDS |
| TCAAATGTGTCATCCTACAT+AGG | 0.567396 | 8.3:-79540726 | MS.gene39665:CDS |
| GAGTCCCTTCAATCAGTCTC+CGG | 0.567399 | 8.3:+79541314 | None:intergenic |
| CCACCGACGACAGCGACGGA+AGG | 0.567420 | 8.3:+79547122 | None:intergenic |
| GCCGAGTTTCTTCATAGACC+AGG | 0.569190 | 8.3:-79546194 | MS.gene39665:CDS |
| TCAAAGAGCATTTAGATGGA+GGG | 0.572110 | 8.3:-79541998 | MS.gene39665:intron |
| CACACAGATAGGAAAGAAAG+AGG | 0.572122 | 8.3:-79540610 | MS.gene39665:intron |
| TGATGCTATCAAAATTTCCA+GGG | 0.575484 | 8.3:-79542795 | MS.gene39665:CDS |
| GGAAGCTTACGTAAAGCAGC+AGG | 0.575667 | 8.3:+79541430 | None:intergenic |
| CCTCAAGAAATGGAGAAAGC+TGG | 0.577336 | 8.3:-79540870 | MS.gene39665:CDS |
| CCTTCCGTCGCTGTCGTCGG+TGG | 0.577628 | 8.3:-79547122 | MS.gene39665:CDS |
| ATCCTACATAGGAATGGTGT+CGG | 0.589525 | 8.3:-79540715 | MS.gene39665:CDS |
| GGACAAAGGAACTAATGCAT+TGG | 0.589827 | 8.3:-79542630 | MS.gene39665:CDS |
| AGAAGTTGCTATGTCCTGGT+TGG | 0.591320 | 8.3:+79542817 | None:intergenic |
| TTTGCGGCAAAGTTCAAGGA+TGG | 0.594840 | 8.3:+79542034 | None:intergenic |
| ACTAATCCATCTAACCGTGC+TGG | 0.596233 | 8.3:-79540795 | MS.gene39665:CDS |
| TCCTGGTCTATGAAGAAACT+CGG | 0.599253 | 8.3:+79546193 | None:intergenic |
| AATGGTGTCGGACACACTCA+GGG | 0.601246 | 8.3:-79540703 | MS.gene39665:CDS |
| TTATGAAGTTGTAACACCAA+TGG | 0.601894 | 8.3:+79546242 | None:intergenic |
| TAAGTGGCCATAGTCAGGAC+TGG | 0.601929 | 8.3:+79542400 | None:intergenic |
| ATGTTAACTGTCCAGGTGAG+AGG | 0.603386 | 8.3:-79542685 | MS.gene39665:intron |
| TATTCGGCTTGTGGATATGG+AGG | 0.603654 | 8.3:-79540928 | MS.gene39665:CDS |
| ATTAGCTGCAGCTACAACTG+AGG | 0.604264 | 8.3:-79540988 | MS.gene39665:CDS |
| TGTGTCATCCTACATAGGAA+TGG | 0.605269 | 8.3:-79540721 | MS.gene39665:CDS |
| CGTCTGCAACATCCTTGGGT+CGG | 0.605481 | 8.3:-79542506 | MS.gene39665:CDS |
| GGTTACCGGAGACTGATTGA+AGG | 0.605725 | 8.3:-79541319 | MS.gene39665:CDS |
| GTCCGACACCATTCCTATGT+AGG | 0.606800 | 8.3:+79540713 | None:intergenic |
| TTGAACTATATAAAGCAGCA+AGG | 0.610358 | 8.3:-79539785 | MS.gene39665:CDS |
| ATACTGATATGATTGTTGCC+AGG | 0.611071 | 8.3:-79542448 | MS.gene39665:CDS |
| TATGATTGTTGCCAGGCGCA+AGG | 0.611351 | 8.3:-79542441 | MS.gene39665:CDS |
| GTTACCGGAGACTGATTGAA+GGG | 0.613786 | 8.3:-79541318 | MS.gene39665:CDS |
| CTGGCCACCGACGACAGCGA+CGG | 0.614825 | 8.3:+79547118 | None:intergenic |
| ACTTCATAAGATAGAAGAAG+GGG | 0.619742 | 8.3:-79546228 | MS.gene39665:CDS |
| CCTACTAATCCATCTAATTG+GGG | 0.619993 | 8.3:-79540834 | MS.gene39665:CDS |
| TCTTCAGAATGTGAAGAGAG+TGG | 0.620832 | 8.3:-79541389 | MS.gene39665:CDS |
| AGACGAAGACCCTACCGTGA+TGG | 0.621898 | 8.3:-79539835 | MS.gene39665:CDS |
| ACCAATTGCTGTTGATGCGG+GGG | 0.622770 | 8.3:-79542162 | MS.gene39665:intron |
| TGCTCTGGGGCAATTAAGTG+AGG | 0.623821 | 8.3:+79541343 | None:intergenic |
| TGAACTATATAAAGCAGCAA+GGG | 0.626349 | 8.3:-79539784 | MS.gene39665:CDS |
| AAGCTGGATTTGATGGACAA+AGG | 0.627483 | 8.3:-79542644 | MS.gene39665:CDS |
| GGGTTGACTAAAGTTGCTGT+AGG | 0.631196 | 8.3:-79543919 | MS.gene39665:intron |
| TAACTTTGATTGATTTGCCG+GGG | 0.635039 | 8.3:-79543939 | MS.gene39665:CDS |
| AGAGTGGTGTCAGAGGCAGA+TGG | 0.635211 | 8.3:-79541373 | MS.gene39665:CDS |
| AACTTCATAAGATAGAAGAA+GGG | 0.635239 | 8.3:-79546229 | MS.gene39665:CDS |
| CGATTTACTACACCGACCCA+AGG | 0.635734 | 8.3:+79542494 | None:intergenic |
| TGGAGTCGGTAATTAGGGCA+CGG | 0.636955 | 8.3:-79542256 | MS.gene39665:CDS |
| AAATTGCAGGGATTGTGACG+AGG | 0.638817 | 8.3:-79546268 | MS.gene39665:intron |
| GCAGGCTAAACAACTATCAG+AGG | 0.640315 | 8.3:-79539862 | MS.gene39665:intron |
| AAAATCAATAGGTGAAACTG+AGG | 0.644678 | 8.3:-79541128 | MS.gene39665:intron |
| GGAGGCTTCGTATCTTACTG+TGG | 0.644824 | 8.3:-79540910 | MS.gene39665:CDS |
| ACTCAGGGTCACTATTCCAA+AGG | 0.654601 | 8.3:-79540688 | MS.gene39665:CDS |
| TTGTTGTCTTCTCTCCATCA+CGG | 0.655926 | 8.3:+79539821 | None:intergenic |
| GGACGTGACTTTCTACCTAG+AGG | 0.662283 | 8.3:-79546955 | MS.gene39665:CDS |
| CTACAACTGAGGCTCTAGAG+AGG | 0.662304 | 8.3:-79540977 | MS.gene39665:CDS |
| AGTTTCTTCATAGACCAGGA+AGG | 0.662508 | 8.3:-79546190 | MS.gene39665:CDS |
| AAATGACGATCGTTACGGTG+AGG | 0.670553 | 8.3:-79540769 | MS.gene39665:CDS |
| GATCGTCATTTATGCCAGCA+CGG | 0.674076 | 8.3:+79540781 | None:intergenic |
| GATTGTTGCCAGGCGCAAGG+AGG | 0.689806 | 8.3:-79542438 | MS.gene39665:CDS |
| ACAGCAACTTTAGTCAACCC+CGG | 0.696272 | 8.3:+79543922 | None:intergenic |
| TATTGTCACAGGCGACCAGG+AGG | 0.697345 | 8.3:-79541487 | MS.gene39665:intron |
| ACTAAACTCGGTGACTATGG+CGG | 0.703039 | 8.3:-79547188 | MS.gene39665:CDS |
| TTGTCTTCTTACTCTCATCG+CGG | 0.704669 | 8.3:+79540952 | None:intergenic |
| TCTCCGGTAACCTTGCTCTG+GGG | 0.721661 | 8.3:+79541330 | None:intergenic |
| GGTACATTAACATACCTGAG+GGG | 0.808362 | 8.3:+79542765 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 79539752 | 79547264 | 79539752 | ID=MS.gene39665 |
| chr8.3 | mRNA | 79539752 | 79547264 | 79539752 | ID=MS.gene39665.t1;Parent=MS.gene39665 |
| chr8.3 | exon | 79547118 | 79547264 | 79547118 | ID=MS.gene39665.t1.exon1;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79547118 | 79547264 | 79547118 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79546950 | 79547013 | 79546950 | ID=MS.gene39665.t1.exon2;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79546950 | 79547013 | 79546950 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79546174 | 79546281 | 79546174 | ID=MS.gene39665.t1.exon3;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79546174 | 79546281 | 79546174 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79545488 | 79545589 | 79545488 | ID=MS.gene39665.t1.exon4;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79545488 | 79545589 | 79545488 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79543920 | 79543970 | 79543920 | ID=MS.gene39665.t1.exon5;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79543920 | 79543970 | 79543920 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79543748 | 79543809 | 79543748 | ID=MS.gene39665.t1.exon6;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79543748 | 79543809 | 79543748 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79542780 | 79542870 | 79542780 | ID=MS.gene39665.t1.exon7;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79542780 | 79542870 | 79542780 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79542628 | 79542692 | 79542628 | ID=MS.gene39665.t1.exon8;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79542628 | 79542692 | 79542628 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79542355 | 79542549 | 79542355 | ID=MS.gene39665.t1.exon9;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79542355 | 79542549 | 79542355 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79542163 | 79542282 | 79542163 | ID=MS.gene39665.t1.exon10;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79542163 | 79542282 | 79542163 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79541999 | 79542069 | 79541999 | ID=MS.gene39665.t1.exon11;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79541999 | 79542069 | 79541999 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79541273 | 79541498 | 79541273 | ID=MS.gene39665.t1.exon12;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79541273 | 79541498 | 79541273 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79541129 | 79541179 | 79541129 | ID=MS.gene39665.t1.exon13;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79541129 | 79541179 | 79541129 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79540611 | 79541042 | 79540611 | ID=MS.gene39665.t1.exon14;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79540611 | 79541042 | 79540611 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| chr8.3 | exon | 79539752 | 79539880 | 79539752 | ID=MS.gene39665.t1.exon15;Parent=MS.gene39665.t1 |
| chr8.3 | CDS | 79539752 | 79539880 | 79539752 | ID=cds.MS.gene39665.t1;Parent=MS.gene39665.t1 |
| Gene Sequence |
| Protein sequence |
