Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034467.t1 | XP_003596800.1 | 99.4 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 1.00E-94 | 355.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034467.t1 | Q9XH43 | 76.9 | 173 | 39 | 1 | 2 | 173 | 3 | 175 | 2.2e-77 | 289.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034467.t1 | G7ITR2 | 99.4 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 7.6e-95 | 355.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene51913 | MS.gene034467 | PPI |
| MS.gene62616 | MS.gene034467 | PPI |
| MS.gene034467 | MS.gene93778 | PPI |
| MS.gene98252 | MS.gene034467 | PPI |
| MS.gene034467 | MS.gene040147 | PPI |
| MS.gene51950 | MS.gene034467 | PPI |
| MS.gene94652 | MS.gene034467 | PPI |
| MS.gene034467 | MS.gene51950 | PPI |
| MS.gene000759 | MS.gene034467 | PPI |
| MS.gene95893 | MS.gene034467 | PPI |
| MS.gene042580 | MS.gene034467 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034467.t1 | MTR_2g086270 | 99.422 | 173 | 1 | 0 | 1 | 173 | 1 | 173 | 5.04e-128 | 356 |
| MS.gene034467.t1 | MTR_7g104460 | 76.786 | 168 | 39 | 0 | 6 | 173 | 7 | 174 | 4.63e-100 | 286 |
| MS.gene034467.t1 | MTR_0020s0120 | 65.896 | 173 | 58 | 1 | 1 | 173 | 1 | 172 | 5.10e-85 | 248 |
| MS.gene034467.t1 | MTR_1g060190 | 67.485 | 163 | 52 | 1 | 2 | 163 | 3 | 165 | 2.00e-81 | 239 |
| MS.gene034467.t1 | MTR_0020s0120 | 63.006 | 173 | 56 | 2 | 1 | 173 | 1 | 165 | 3.94e-78 | 230 |
| MS.gene034467.t1 | MTR_7g084970 | 55.357 | 168 | 74 | 1 | 6 | 173 | 7 | 173 | 5.96e-67 | 202 |
| MS.gene034467.t1 | MTR_6g033040 | 52.976 | 168 | 78 | 1 | 6 | 173 | 7 | 173 | 9.54e-62 | 189 |
| MS.gene034467.t1 | MTR_7g085020 | 54.762 | 168 | 75 | 1 | 6 | 173 | 8 | 174 | 1.22e-60 | 186 |
| MS.gene034467.t1 | MTR_7g006690 | 49.412 | 170 | 84 | 2 | 5 | 173 | 6 | 174 | 5.33e-58 | 179 |
| MS.gene034467.t1 | MTR_8g106840 | 49.133 | 173 | 87 | 1 | 1 | 173 | 2 | 173 | 9.27e-57 | 176 |
| MS.gene034467.t1 | MTR_7g006630 | 49.112 | 169 | 84 | 2 | 6 | 173 | 2 | 169 | 1.48e-56 | 176 |
| MS.gene034467.t1 | MTR_7g085040 | 49.412 | 170 | 81 | 3 | 4 | 173 | 5 | 169 | 5.90e-54 | 169 |
| MS.gene034467.t1 | MTR_5g078320 | 38.356 | 73 | 45 | 0 | 100 | 172 | 16 | 88 | 2.19e-13 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034467.t1 | AT2G27550 | 76.163 | 172 | 40 | 1 | 3 | 173 | 4 | 175 | 1.18e-98 | 282 |
| MS.gene034467.t1 | AT5G03840 | 73.529 | 170 | 45 | 0 | 4 | 173 | 8 | 177 | 3.39e-94 | 271 |
| MS.gene034467.t1 | AT5G62040 | 57.471 | 174 | 73 | 1 | 1 | 173 | 1 | 174 | 4.49e-73 | 218 |
| MS.gene034467.t1 | AT1G65480 | 54.023 | 174 | 78 | 2 | 1 | 173 | 1 | 173 | 1.46e-64 | 196 |
| MS.gene034467.t1 | AT1G65480 | 54.023 | 174 | 78 | 2 | 1 | 173 | 45 | 217 | 3.29e-64 | 196 |
| MS.gene034467.t1 | AT4G20370 | 53.571 | 168 | 77 | 1 | 6 | 173 | 7 | 173 | 3.76e-60 | 185 |
| MS.gene034467.t1 | AT1G18100 | 50.000 | 174 | 85 | 2 | 1 | 173 | 1 | 173 | 2.28e-56 | 175 |
Find 39 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCATGAATCTGAACTTT+AGG | 0.259268 | 2.3:-12618799 | None:intergenic |
| TCACTAGGGCCAGGGACATC+TGG | 0.386750 | 2.3:-12618984 | None:intergenic |
| CTTGCTACTGGGAGAGTGAT+TGG | 0.388594 | 2.3:+12618670 | MS.gene034467:CDS |
| AAACCTAAAGTTCAGATTCA+TGG | 0.412345 | 2.3:+12618796 | MS.gene034467:CDS |
| GTAACAGATCCTCTTGCTAC+TGG | 0.433455 | 2.3:+12618658 | MS.gene034467:CDS |
| GGCACAACTGATGCTACATT+TGG | 0.461577 | 2.3:+12619925 | MS.gene034467:CDS |
| TCCTTCAGGTACGGATCACT+AGG | 0.461865 | 2.3:-12618999 | None:intergenic |
| GGTTTAGTAGTAACTGAGGA+AGG | 0.479372 | 2.3:-12618778 | None:intergenic |
| TGCGCTTCTGCTTGTAAAGA+AGG | 0.483125 | 2.3:-12620128 | None:intergenic |
| AACCTATGGATTCCTATGTT+AGG | 0.490109 | 2.3:-12620099 | None:intergenic |
| ACCAGTGTAAGTGTTCCTTC+AGG | 0.517412 | 2.3:-12619013 | None:intergenic |
| GATTTGTACCAGAGTGAAGA+AGG | 0.531332 | 2.3:-12618833 | None:intergenic |
| AAAGAAGGAACACAAACCTA+TGG | 0.532641 | 2.3:-12620113 | None:intergenic |
| TGATGAAAATACCAACATCA+AGG | 0.533106 | 2.3:+12620160 | MS.gene034467:CDS |
| AGGTACGGATCACTAGGGCC+AGG | 0.551194 | 2.3:-12618993 | None:intergenic |
| AATGTAGCATCAGTTGTGCC+TGG | 0.566122 | 2.3:-12619922 | None:intergenic |
| TGGATTCCTATGTTAGGTCG+TGG | 0.569225 | 2.3:-12620093 | None:intergenic |
| TAACAGATCCTCTTGCTACT+GGG | 0.573755 | 2.3:+12618659 | MS.gene034467:CDS |
| CAATGACCTCGGGCCTCCTG+TGG | 0.580778 | 2.3:+12620215 | MS.gene034467:CDS |
| CCCTAGTGATCCGTACCTGA+AGG | 0.582765 | 2.3:+12618998 | MS.gene034467:CDS |
| TGTAAGTGTTCCTTCAGGTA+CGG | 0.588171 | 2.3:-12619008 | None:intergenic |
| GAAATGCCACGACCTAACAT+AGG | 0.588896 | 2.3:+12620087 | MS.gene034467:CDS |
| ATGACAGATCCAGATGTCCC+TGG | 0.589097 | 2.3:+12618975 | MS.gene034467:CDS |
| AGGATCTGTTACAATACTCA+TGG | 0.589317 | 2.3:-12618647 | None:intergenic |
| GATGAAAATACCAACATCAA+GGG | 0.592305 | 2.3:+12620161 | MS.gene034467:CDS |
| ATCACTCTCCCAGTAGCAAG+AGG | 0.600948 | 2.3:-12618667 | None:intergenic |
| TATGAGATCCTTCTTCACTC+TGG | 0.602356 | 2.3:+12618825 | MS.gene034467:CDS |
| TTGCACAAGACAATGACCTC+GGG | 0.602430 | 2.3:+12620205 | MS.gene034467:CDS |
| AACACTAAGCAAGTTTACAA+TGG | 0.606083 | 2.3:+12618742 | MS.gene034467:CDS |
| CAGCAGCCACAGGAGGCCCG+AGG | 0.611106 | 2.3:-12620221 | None:intergenic |
| TTAAAGAGATCCCTTGATGT+TGG | 0.626156 | 2.3:-12620171 | None:intergenic |
| GGTACGGATCACTAGGGCCA+GGG | 0.633775 | 2.3:-12618992 | None:intergenic |
| ACCTGAAGGAACACTTACAC+TGG | 0.635472 | 2.3:+12619012 | MS.gene034467:CDS |
| TTTAGGTTTAGTAGTAACTG+AGG | 0.640423 | 2.3:-12618782 | None:intergenic |
| TTTGCACAAGACAATGACCT+CGG | 0.646965 | 2.3:+12620204 | MS.gene034467:CDS |
| CCTTCAGGTACGGATCACTA+GGG | 0.665821 | 2.3:-12618998 | None:intergenic |
| AGGATAGTCACAGATATACC+AGG | 0.670510 | 2.3:+12619904 | MS.gene034467:intron |
| GACCTAACATAGGAATCCAT+AGG | 0.672986 | 2.3:+12620097 | MS.gene034467:CDS |
| CCTAAAGTTCAGATTCATGG+TGG | 0.696957 | 2.3:+12618799 | MS.gene034467:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATTAGAAAAGAATTAA+AGG | + | chr2.3:12619336-12619355 | MS.gene034467:intron | 10.0% |
| !! | ATAATTTAAGTAATATAGAT+AGG | + | chr2.3:12619256-12619275 | MS.gene034467:intron | 10.0% |
| !! | TATTCAAATTTACTAAATAT+GGG | + | chr2.3:12619536-12619555 | MS.gene034467:intron | 10.0% |
| !! | TTATTCAAATTTACTAAATA+TGG | + | chr2.3:12619535-12619554 | MS.gene034467:intron | 10.0% |
| !!! | AAATTCTAATTTTTTTTCTT+CGG | - | chr2.3:12619308-12619327 | None:intergenic | 10.0% |
| !!! | AAATTTGTAATTTTTTTTTG+AGG | - | chr2.3:12619764-12619783 | None:intergenic | 10.0% |
| !!! | AATTTGTAATTTTTTTTTGA+GGG | - | chr2.3:12619763-12619782 | None:intergenic | 10.0% |
| !!! | CTCAACTTTTTATTAAAAAA+TGG | - | chr2.3:12619475-12619494 | None:intergenic | 15.0% |
| !!! | TCAATATATTCTATTTTTTC+AGG | + | chr2.3:12618950-12618969 | MS.gene034467:intron | 15.0% |
| !!! | TTTTCACTTTAATTAATACA+TGG | + | chr2.3:12620000-12620019 | MS.gene034467:intron | 15.0% |
| !! | AAATAGAATATATTGAAGCA+AGG | - | chr2.3:12618946-12618965 | None:intergenic | 20.0% |
| !! | AAATATGGATTTGTCTAATA+TGG | + | chr2.3:12619430-12619449 | MS.gene034467:intron | 20.0% |
| !! | TCATAACAAATGCTTATTAT+AGG | - | chr2.3:12619217-12619236 | None:intergenic | 20.0% |
| !!! | AAACAATGAACAAACTTTTT+TGG | + | chr2.3:12619400-12619419 | MS.gene034467:intron | 20.0% |
| !!! | AGCATTTGTTATGAAATATA+AGG | + | chr2.3:12619223-12619242 | MS.gene034467:intron | 20.0% |
| !!! | TTTTTTGGTGATCTAAAATA+TGG | + | chr2.3:12619415-12619434 | MS.gene034467:intron | 20.0% |
| ! | ACAACTCTTATATATTCTCA+CGG | + | chr2.3:12619690-12619709 | MS.gene034467:intron | 25.0% |
| ! | ATTATTAACTCAAATCTGTG+TGG | + | chr2.3:12619089-12619108 | MS.gene034467:intron | 25.0% |
| ! | ATTTGGTAAGTCCTTTAATA+TGG | + | chr2.3:12619942-12619961 | MS.gene034467:intron | 25.0% |
| ! | GAAATAAAGAAGTAGTGATT+TGG | - | chr2.3:12618864-12618883 | None:intergenic | 25.0% |
| ! | TACTTCATTGAAAATGAACA+GGG | + | chr2.3:12620046-12620065 | MS.gene034467:intron | 25.0% |
| ! | TTACTTCATTGAAAATGAAC+AGG | + | chr2.3:12620045-12620064 | MS.gene034467:intron | 25.0% |
| !! | AAAAAATGGTAGACTCTTTT+TGG | - | chr2.3:12619461-12619480 | None:intergenic | 25.0% |
| !! | AAAAATGGTAGACTCTTTTT+GGG | - | chr2.3:12619460-12619479 | None:intergenic | 25.0% |
| !! | AGCATTGAACATGATAAAAA+AGG | - | chr2.3:12619566-12619585 | None:intergenic | 25.0% |
| !! | TCATTGTTTATATTTGAGAC+TGG | - | chr2.3:12619389-12619408 | None:intergenic | 25.0% |
| AAACCTAAAGTTCAGATTCA+TGG | + | chr2.3:12618796-12618815 | MS.gene034467:CDS | 30.0% | |
| AACACTAAGCAAGTTTACAA+TGG | + | chr2.3:12618742-12618761 | MS.gene034467:CDS | 30.0% | |
| AATTGACGTGACCATATTAA+AGG | - | chr2.3:12619956-12619975 | None:intergenic | 30.0% | |
| CTGTGATGCACAATTAAAAT+AGG | - | chr2.3:12619886-12619905 | None:intergenic | 30.0% | |
| GATGAAAATACCAACATCAA+GGG | + | chr2.3:12620161-12620180 | MS.gene034467:CDS | 30.0% | |
| TGATGAAAATACCAACATCA+AGG | + | chr2.3:12620160-12620179 | MS.gene034467:CDS | 30.0% | |
| ! | TTTAGGTTTAGTAGTAACTG+AGG | - | chr2.3:12618785-12618804 | None:intergenic | 30.0% |
| !!! | CTATTTTAATTGTGCATCAC+AGG | + | chr2.3:12619884-12619903 | MS.gene034467:intron | 30.0% |
| AAAGAAGGAACACAAACCTA+TGG | - | chr2.3:12620116-12620135 | None:intergenic | 35.0% | |
| AACCTATGGATTCCTATGTT+AGG | - | chr2.3:12620102-12620121 | None:intergenic | 35.0% | |
| TATTGAAGCAAGGAAACTCA+AGG | - | chr2.3:12618936-12618955 | None:intergenic | 35.0% | |
| TTAAAGAGATCCCTTGATGT+TGG | - | chr2.3:12620174-12620193 | None:intergenic | 35.0% | |
| ! | GTTTATATTTGAGACTGGAG+TGG | - | chr2.3:12619384-12619403 | None:intergenic | 35.0% |
| AGGATAGTCACAGATATACC+AGG | + | chr2.3:12619904-12619923 | MS.gene034467:intron | 40.0% | |
| CCACCATGAATCTGAACTTT+AGG | - | chr2.3:12618802-12618821 | None:intergenic | 40.0% | |
| CCTAAAGTTCAGATTCATGG+TGG | + | chr2.3:12618799-12618818 | MS.gene034467:CDS | 40.0% | |
| GACCTAACATAGGAATCCAT+AGG | + | chr2.3:12620097-12620116 | MS.gene034467:CDS | 40.0% | |
| GATATACTTGCATGCATCAG+AGG | - | chr2.3:12619195-12619214 | None:intergenic | 40.0% | |
| GATTTGTACCAGAGTGAAGA+AGG | - | chr2.3:12618836-12618855 | None:intergenic | 40.0% | |
| GGTTTAGTAGTAACTGAGGA+AGG | - | chr2.3:12618781-12618800 | None:intergenic | 40.0% | |
| TAACAGATCCTCTTGCTACT+GGG | + | chr2.3:12618659-12618678 | MS.gene034467:CDS | 40.0% | |
| TATGAGATCCTTCTTCACTC+TGG | + | chr2.3:12618825-12618844 | MS.gene034467:CDS | 40.0% | |
| TCAAATCTGTGTGGTTTGAG+TGG | + | chr2.3:12619098-12619117 | MS.gene034467:intron | 40.0% | |
| TGTAAGTGTTCCTTCAGGTA+CGG | - | chr2.3:12619011-12619030 | None:intergenic | 40.0% | |
| TTGAAAAAAACAGCAGCCAC+AGG | - | chr2.3:12620234-12620253 | None:intergenic | 40.0% | |
| TTTGCACAAGACAATGACCT+CGG | + | chr2.3:12620204-12620223 | MS.gene034467:CDS | 40.0% | |
| AAAAAAACAGCAGCCACAGG+AGG | - | chr2.3:12620231-12620250 | None:intergenic | 45.0% | |
| AATGTAGCATCAGTTGTGCC+TGG | - | chr2.3:12619925-12619944 | None:intergenic | 45.0% | |
| ACCAGTGTAAGTGTTCCTTC+AGG | - | chr2.3:12619016-12619035 | None:intergenic | 45.0% | |
| ACCTGAAGGAACACTTACAC+TGG | + | chr2.3:12619012-12619031 | MS.gene034467:CDS | 45.0% | |
| AGCAAGGAAACTCAAGGACA+AGG | - | chr2.3:12618930-12618949 | None:intergenic | 45.0% | |
| GAAATGCCACGACCTAACAT+AGG | + | chr2.3:12620087-12620106 | MS.gene034467:CDS | 45.0% | |
| GGCACAACTGATGCTACATT+TGG | + | chr2.3:12619925-12619944 | MS.gene034467:CDS | 45.0% | |
| GTAACAGATCCTCTTGCTAC+TGG | + | chr2.3:12618658-12618677 | MS.gene034467:CDS | 45.0% | |
| TGCGCTTCTGCTTGTAAAGA+AGG | - | chr2.3:12620131-12620150 | None:intergenic | 45.0% | |
| TGGATTCCTATGTTAGGTCG+TGG | - | chr2.3:12620096-12620115 | None:intergenic | 45.0% | |
| TTGCACAAGACAATGACCTC+GGG | + | chr2.3:12620205-12620224 | MS.gene034467:CDS | 45.0% | |
| ATCACTCTCCCAGTAGCAAG+AGG | - | chr2.3:12618670-12618689 | None:intergenic | 50.0% | |
| ATGACAGATCCAGATGTCCC+TGG | + | chr2.3:12618975-12618994 | MS.gene034467:CDS | 50.0% | |
| CCTTCAGGTACGGATCACTA+GGG | - | chr2.3:12619001-12619020 | None:intergenic | 50.0% | |
| CTTGCTACTGGGAGAGTGAT+TGG | + | chr2.3:12618670-12618689 | MS.gene034467:CDS | 50.0% | |
| TCCTTCAGGTACGGATCACT+AGG | - | chr2.3:12619002-12619021 | None:intergenic | 50.0% | |
| CCCTAGTGATCCGTACCTGA+AGG | + | chr2.3:12618998-12619017 | MS.gene034467:CDS | 55.0% | |
| AGGTACGGATCACTAGGGCC+AGG | - | chr2.3:12618996-12619015 | None:intergenic | 60.0% | |
| GGTACGGATCACTAGGGCCA+GGG | - | chr2.3:12618995-12619014 | None:intergenic | 60.0% | |
| TCACTAGGGCCAGGGACATC+TGG | - | chr2.3:12618987-12619006 | None:intergenic | 60.0% | |
| CAATGACCTCGGGCCTCCTG+TGG | + | chr2.3:12620215-12620234 | MS.gene034467:CDS | 65.0% | |
| CAGCAGCCACAGGAGGCCCG+AGG | - | chr2.3:12620224-12620243 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 12618649 | 12620287 | 12618649 | ID=MS.gene034467 |
| chr2.3 | mRNA | 12618649 | 12620287 | 12618649 | ID=MS.gene034467.t1;Parent=MS.gene034467 |
| chr2.3 | exon | 12618649 | 12618846 | 12618649 | ID=MS.gene034467.t1.exon1;Parent=MS.gene034467.t1 |
| chr2.3 | CDS | 12618649 | 12618846 | 12618649 | ID=cds.MS.gene034467.t1;Parent=MS.gene034467.t1 |
| chr2.3 | exon | 12618972 | 12619033 | 12618972 | ID=MS.gene034467.t1.exon2;Parent=MS.gene034467.t1 |
| chr2.3 | CDS | 12618972 | 12619033 | 12618972 | ID=cds.MS.gene034467.t1;Parent=MS.gene034467.t1 |
| chr2.3 | exon | 12619906 | 12619946 | 12619906 | ID=MS.gene034467.t1.exon3;Parent=MS.gene034467.t1 |
| chr2.3 | CDS | 12619906 | 12619946 | 12619906 | ID=cds.MS.gene034467.t1;Parent=MS.gene034467.t1 |
| chr2.3 | exon | 12620067 | 12620287 | 12620067 | ID=MS.gene034467.t1.exon4;Parent=MS.gene034467.t1 |
| chr2.3 | CDS | 12620067 | 12620287 | 12620067 | ID=cds.MS.gene034467.t1;Parent=MS.gene034467.t1 |
| Gene Sequence |
| Protein sequence |
