AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene040704

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040704.t1 MTR_5g022730 98.052 154 3 0 3 156 2 155 2.71e-110 310
MS.gene040704.t1 MTR_5g022710 96.104 154 6 0 3 156 2 155 1.92e-108 305
MS.gene040704.t1 MTR_7g021400 87.179 156 20 0 1 156 1 156 5.63e-93 266
MS.gene040704.t1 MTR_7g094150 86.538 156 21 0 1 156 1 156 1.58e-91 263
MS.gene040704.t1 MTR_7g094170 85.256 156 23 0 1 156 1 156 5.22e-90 259
MS.gene040704.t1 MTR_7g021380 83.333 156 26 0 1 156 1 156 4.29e-86 249
MS.gene040704.t1 MTR_3g110048 76.582 158 35 1 1 156 1 158 1.20e-85 248
MS.gene040704.t1 MTR_4g032400 78.846 156 32 1 2 156 8 163 1.40e-85 248
MS.gene040704.t1 MTR_7g094120 75.159 157 37 1 2 156 3 159 5.24e-84 244
MS.gene040704.t1 MTR_4g032450 73.885 157 40 1 1 156 19 175 6.38e-84 244
MS.gene040704.t1 MTR_8g056060 73.885 157 40 1 1 156 1 157 1.82e-79 232
MS.gene040704.t1 MTR_7g094280 72.152 158 42 2 1 156 1 158 6.79e-79 231
MS.gene040704.t1 MTR_5g085030 72.078 154 42 1 3 155 2 155 2.49e-77 227
MS.gene040704.t1 MTR_8g468480 69.677 155 46 1 3 156 2 156 5.67e-76 223
MS.gene040704.t1 MTR_4g032510 68.790 157 41 3 2 156 3 153 3.14e-71 211
MS.gene040704.t1 MTR_7g094310 68.987 158 37 3 1 156 1 148 4.14e-71 211
MS.gene040704.t1 MTR_4g032460 72.258 155 36 2 3 156 2 150 1.90e-70 209
MS.gene040704.t1 MTR_4g032500 66.667 156 45 2 2 156 3 152 1.07e-69 207
MS.gene040704.t1 MTR_4g033455 60.390 154 61 0 3 156 7 160 5.82e-65 196
MS.gene040704.t1 MTR_5g082340 66.452 155 40 2 3 156 2 145 3.85e-64 193
MS.gene040704.t1 MTR_4g017860 56.410 195 42 4 5 156 26 220 1.71e-63 194
MS.gene040704.t1 MTR_1g099870 84.314 102 14 1 57 156 2 103 6.75e-59 178
MS.gene040704.t1 MTR_4g021390 58.710 155 61 1 2 156 4 155 1.11e-58 179
MS.gene040704.t1 MTR_4g033460 60.000 140 56 0 3 142 7 146 2.42e-58 182
MS.gene040704.t1 MTR_4g033460 60.215 93 29 1 41 133 161 245 1.79e-30 110
MS.gene040704.t1 MTR_8g089730 69.919 123 20 1 34 156 1 106 2.73e-50 157
MS.gene040704.t1 MTR_7g094320 56.051 157 28 2 1 156 1 117 1.84e-48 152
MS.gene040704.t1 MTR_4g032420 61.417 127 34 3 31 156 38 150 6.05e-47 150
MS.gene040704.t1 MTR_7g006370 54.610 141 55 2 2 142 4 135 8.42e-44 141
MS.gene040704.t1 MTR_4g032470 51.370 146 27 2 12 156 32 134 1.13e-43 141
MS.gene040704.t1 MTR_7g094410 53.846 156 30 4 1 114 1 156 1.17e-41 137
MS.gene040704.t1 MTR_4g127240 66.990 103 28 4 40 140 26 124 1.90e-40 132
MS.gene040704.t1 MTR_4g131980 63.866 119 37 3 3 119 2 116 2.87e-40 132
MS.gene040704.t1 MTR_7g094360 47.093 172 38 4 1 119 1 172 7.87e-40 133
MS.gene040704.t1 MTR_8g056090 49.359 156 46 6 1 154 1 125 6.18e-37 124
MS.gene040704.t1 MTR_4g119422 48.980 147 68 2 2 146 227 368 2.71e-35 125
MS.gene040704.t1 MTR_1g112590 39.355 155 78 2 8 146 1 155 1.60e-32 113
MS.gene040704.t1 MTR_7g071200 71.014 69 19 1 89 156 4 72 1.16e-28 100
MS.gene040704.t1 MTR_2g066010 41.722 151 76 5 8 155 20 161 3.02e-27 101
MS.gene040704.t1 MTR_4g032520 44.444 144 38 4 29 154 1 120 3.41e-27 99.4
MS.gene040704.t1 MTR_2g065720 39.873 158 83 5 1 155 30 178 2.82e-26 99.0
MS.gene040704.t1 MTR_7g094350 50.459 109 32 2 1 109 1 87 2.93e-25 95.9
MS.gene040704.t1 MTR_7g094350 51.685 89 39 3 48 135 102 187 7.35e-20 82.0
MS.gene040704.t1 MTR_5g096950 37.736 159 78 4 1 155 1 142 1.71e-23 90.1
MS.gene040704.t1 MTR_4g032540 65.079 63 21 1 90 151 70 132 3.89e-23 88.6
MS.gene040704.t1 MTR_2g018980 34.667 150 85 3 8 156 3 140 2.67e-22 87.0
MS.gene040704.t1 MTR_1g042510 35.256 156 63 4 8 146 1 135 3.93e-22 89.7
MS.gene040704.t1 MTR_1g112570 36.691 139 63 4 8 128 1 132 1.16e-20 85.9
MS.gene040704.t1 MTR_5g078680 32.051 156 68 3 8 146 1 135 2.21e-19 82.0
MS.gene040704.t1 MTR_6g053620 36.806 144 81 5 8 146 17 155 3.46e-19 82.8
MS.gene040704.t1 MTR_6g053620 36.806 144 81 5 8 146 17 155 3.84e-19 82.8
MS.gene040704.t1 MTR_8g007195 34.286 140 80 4 10 143 13 146 2.24e-18 80.1
MS.gene040704.t1 MTR_7g077810 34.722 144 84 4 8 146 18 156 7.78e-18 79.0
MS.gene040704.t1 MTR_2g100825 32.667 150 92 4 1 146 4 148 5.04e-17 76.3
MS.gene040704.t1 MTR_2g100830 33.793 145 80 5 10 146 13 149 7.42e-17 75.9
MS.gene040704.t1 MTR_8g007185 32.877 146 87 4 8 147 11 151 8.12e-17 75.5
MS.gene040704.t1 MTR_8g007205 32.877 146 87 4 8 147 11 151 1.26e-16 75.1
MS.gene040704.t1 MTR_7g036780 34.043 141 82 6 1 135 1 136 1.98e-15 71.2
MS.gene040704.t1 MTR_4g131910 39.286 112 58 2 5 112 133 238 1.15e-14 69.3
MS.gene040704.t1 MTR_8g030670 30.345 145 91 3 10 149 20 159 1.98e-14 69.7
MS.gene040704.t1 MTR_3g061700 33.684 95 56 2 53 146 1 89 1.98e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene040704.t1 AT5G42190 77.059 170 23 1 3 156 2 171 2.43e-85 248
MS.gene040704.t1 AT1G20140 72.393 163 38 2 1 156 1 163 3.63e-83 242
MS.gene040704.t1 AT1G75950 77.987 159 29 1 4 156 2 160 3.74e-83 242
MS.gene040704.t1 AT2G25700 68.712 163 44 2 1 156 1 163 1.85e-78 230
MS.gene040704.t1 AT4G34210 70.130 154 42 2 4 156 2 152 1.78e-76 224
MS.gene040704.t1 AT4G34470 69.481 154 43 2 4 156 2 152 1.11e-75 223
MS.gene040704.t1 AT3G21860 67.097 155 45 3 4 156 2 152 4.85e-68 203
MS.gene040704.t1 AT3G21850 64.516 155 50 3 4 156 2 153 7.02e-67 200
MS.gene040704.t1 AT3G60010 64.331 157 47 3 5 156 2 154 2.10e-66 199
MS.gene040704.t1 AT2G03170 62.338 154 51 2 4 156 2 149 2.27e-63 191
MS.gene040704.t1 AT2G03190 55.422 166 61 3 4 156 2 167 7.23e-59 181
MS.gene040704.t1 AT3G60020 57.692 156 54 3 7 156 4 153 5.59e-57 175
MS.gene040704.t1 AT3G21830 56.494 154 63 2 4 156 2 152 1.04e-55 172
MS.gene040704.t1 AT2G03160 48.677 189 61 3 4 156 2 190 1.05e-55 174
MS.gene040704.t1 AT2G20160 54.902 153 63 2 4 155 2 149 2.07e-53 166
MS.gene040704.t1 AT3G25650 57.229 166 58 5 4 156 2 167 3.07e-53 167
MS.gene040704.t1 AT1G10230 56.774 155 64 2 4 155 2 156 4.76e-52 163
MS.gene040704.t1 AT3G21840 58.333 120 44 3 4 121 2 117 1.72e-37 125
MS.gene040704.t1 AT3G53060 66.279 86 22 3 29 113 1 80 8.25e-32 109
MS.gene040704.t1 AT3G61415 33.333 144 86 4 8 146 18 156 2.46e-16 74.7
MS.gene040704.t1 AT3G61415 33.333 144 86 4 8 146 18 156 2.57e-16 74.7
MS.gene040704.t1 AT2G45950 31.944 144 88 4 8 146 18 156 3.69e-16 74.3
MS.gene040704.t1 AT2G45950 31.944 144 88 4 8 146 18 156 3.69e-16 74.3
MS.gene040704.t1 AT2G45950 31.944 144 88 4 8 146 18 156 3.78e-16 74.3
MS.gene040704.t1 AT2G45950 31.944 144 88 4 8 146 18 156 3.78e-16 74.3
MS.gene040704.t1 AT2G45950 31.944 144 88 4 8 146 18 156 3.78e-16 74.3

Find 38 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAATATCAAGAGCTTGC+TGG 0.358298 1.2:+34301995 MS.gene040704:CDS
GGTCGATGAAGCGGTTGCTT+TGG 0.364221 1.2:+34300287 MS.gene040704:CDS
CAACGTTACCAGCAAGATCT+TGG 0.386921 1.2:+34300371 MS.gene040704:CDS
CGAGGATGAGCTTAAGTCTT+GGG 0.409245 1.2:+34300455 MS.gene040704:CDS
CGAGTTCGTCAAGGTTGATC+AGG 0.435035 1.2:+34300482 MS.gene040704:CDS
CACCTTCGCCAAGATCTTGC+TGG 0.436666 1.2:-34300379 None:intergenic
AATGTCTTGCGGATCTCCTC+TGG 0.448832 1.2:-34302062 None:intergenic
GACTTAAGCTCATCCTCGTT+AGG 0.455978 1.2:-34300450 None:intergenic
TGATCATGTCTGCAACAGTC+TGG 0.461006 1.2:-34302028 None:intergenic
AAGATCTTGCTGGTAACGTT+GGG 0.477857 1.2:-34300369 None:intergenic
CATGTCTGCAACAGTCTGGC+AGG 0.496105 1.2:-34302024 None:intergenic
TGATTTCTCACCTGAGGAAG+AGG 0.505723 1.2:+34302097 MS.gene040704:CDS
TGCTGGTAACGTTGGGAAGA+GGG 0.508109 1.2:-34300362 None:intergenic
TTGCTGGTAACGTTGGGAAG+AGG 0.523250 1.2:-34300363 None:intergenic
CAAGATCTTGCTGGTAACGT+TGG 0.530853 1.2:-34300370 None:intergenic
CGAACTTCCTCCTCTTCCTC+AGG 0.533819 1.2:-34302107 None:intergenic
GACTGTTGCAGACATGATCA+AGG 0.534990 1.2:+34302031 MS.gene040704:CDS
CTGATCAACCTTGACGAACT+CGG 0.543997 1.2:-34300481 None:intergenic
ACGAGGATGAGCTTAAGTCT+TGG 0.544399 1.2:+34300454 MS.gene040704:CDS
AGCATCATTCAAATGCCCAT+TGG 0.546088 1.2:-34302139 None:intergenic
AGGAAGAGGAGGAAGTTCGT+AGG 0.548370 1.2:+34302111 MS.gene040704:CDS
GGAAGAGGAGGAAGTTCGTA+GGG 0.553401 1.2:+34302112 MS.gene040704:CDS
AGTTCGTAGGGAAAACCAAT+GGG 0.562143 1.2:+34302124 MS.gene040704:CDS
AAGTTCGTAGGGAAAACCAA+TGG 0.564773 1.2:+34302123 MS.gene040704:CDS
TCTTGATGTTGAATGTCTTG+CGG 0.592623 1.2:-34302073 None:intergenic
TTGGGATTCCGAGTTCGTCA+AGG 0.598524 1.2:+34300473 MS.gene040704:CDS
GAAGATGATTGCGCTGACAG+TGG 0.607194 1.2:+34300336 MS.gene040704:CDS
GACCTTCGAGGTCGATGAAG+CGG 0.610768 1.2:+34300278 MS.gene040704:CDS
TTCCGACGGTGAGACCTTCG+AGG 0.613590 1.2:+34300266 MS.gene040704:CDS
ATCACTCTGAAGAGTTCCGA+CGG 0.622850 1.2:+34300252 MS.gene040704:CDS
AACCGCTTCATCGACCTCGA+AGG 0.623664 1.2:-34300280 None:intergenic
TACCAGCAAGATCTTGGCGA+AGG 0.627734 1.2:+34300377 MS.gene040704:CDS
TTTCTCACCTGAGGAAGAGG+AGG 0.645844 1.2:+34302100 MS.gene040704:CDS
CAAGAATGATTTCTCACCTG+AGG 0.653595 1.2:+34302091 MS.gene040704:CDS
ACTGTTGCAGACATGATCAA+GGG 0.659883 1.2:+34302032 MS.gene040704:CDS
GACCTCGAAGGTCTCACCGT+CGG 0.662584 1.2:-34300268 None:intergenic
TGCTGAAGACAAACCTAACG+AGG 0.677963 1.2:+34300437 MS.gene040704:CDS
GATCAAGGGAAAGACACCAG+AGG 0.742039 1.2:+34302046 MS.gene040704:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTATTAATAAAAAAT+AGG - chr1.2:34300645-34300664 None:intergenic 0.0%
!!! TTTTTTATTAATAATAATAA+AGG + chr1.2:34300646-34300665 MS.gene040704:intron 0.0%
!! ATTAATAATAATAAAGGATT+AGG + chr1.2:34300652-34300671 MS.gene040704:intron 10.0%
!! TTAATAATAATAAAGGATTA+GGG + chr1.2:34300653-34300672 MS.gene040704:intron 10.0%
!!! ATTTTATTTATGTTTTCATT+TGG + chr1.2:34300983-34301002 MS.gene040704:intron 10.0%
!!! TTATTTAATTTAAATGGTTA+AGG + chr1.2:34301920-34301939 MS.gene040704:intron 10.0%
!! TTTAATTCGTTTATTTGATT+TGG + chr1.2:34300546-34300565 MS.gene040704:intron 15.0%
!!! GGTTTATTCTTTTAAATTTT+AGG + chr1.2:34301681-34301700 MS.gene040704:intron 15.0%
!!! TACTTAGGTTTTTTTAATAA+TGG - chr1.2:34301760-34301779 None:intergenic 15.0%
!!! TTAATTCGTTTATTTGATTT+GGG + chr1.2:34300547-34300566 MS.gene040704:intron 15.0%
!!! TTGACATTTTTTTTCTTTTT+AGG + chr1.2:34301962-34301981 MS.gene040704:intron 15.0%
!! AAAGTTCTATAAAACATTGA+AGG - chr1.2:34301372-34301391 None:intergenic 20.0%
!! AATAAAGAACATATAAAGCA+TGG + chr1.2:34301519-34301538 MS.gene040704:intron 20.0%
!! TTCATAAATCAATTGATGTT+CGG - chr1.2:34301463-34301482 None:intergenic 20.0%
!! TTCTAATGTATTTGATTAAG+TGG + chr1.2:34301211-34301230 MS.gene040704:intron 20.0%
!! TTGAAATTGAAAAAACTTCA+AGG - chr1.2:34300724-34300743 None:intergenic 20.0%
!! TTGTTATCTGAATAGTTTAT+TGG + chr1.2:34300615-34300634 MS.gene040704:intron 20.0%
!!! ATGTTTATGTTATGTTTTGT+TGG + chr1.2:34301069-34301088 MS.gene040704:intron 20.0%
!!! ATTTCAATTTGTCTTTTGTT+TGG + chr1.2:34300737-34300756 MS.gene040704:intron 20.0%
!!! TAAGGCTGATTTTATATTAT+TGG + chr1.2:34301938-34301957 MS.gene040704:intron 20.0%
!!! TTTCAATTTGTCTTTTGTTT+GGG + chr1.2:34300738-34300757 MS.gene040704:intron 20.0%
! GTATGATATCATTGTTTGTA+TGG + chr1.2:34301400-34301419 MS.gene040704:intron 25.0%
! TAACAAGAAAACCCTAATTT+AGG - chr1.2:34300601-34300620 None:intergenic 25.0%
! TTAAAAAAACCTAAGTAGTC+TGG + chr1.2:34301763-34301782 MS.gene040704:intron 25.0%
!! ACATTGTCTCAATATTTTGT+TGG - chr1.2:34301571-34301590 None:intergenic 25.0%
!! TAATAAAGGATTAGGGTTTT+TGG + chr1.2:34300660-34300679 MS.gene040704:intron 25.0%
!!! ATCTTGGTAAGTTTGTTTTA+GGG + chr1.2:34300522-34300541 MS.gene040704:intron 25.0%
!!! ATTAAGTGGTTCTAATTTTC+AGG + chr1.2:34301225-34301244 MS.gene040704:intron 25.0%
!!! ATTTGATTTGGGTTTTGTTT+TGG + chr1.2:34300558-34300577 MS.gene040704:intron 25.0%
!!! TTGTTTGGGTTGAATTTAAT+GGG + chr1.2:34300752-34300771 MS.gene040704:intron 25.0%
!!! TTTGTTTGGGTTGAATTTAA+TGG + chr1.2:34300751-34300770 MS.gene040704:intron 25.0%
AACAAACAAACAGCCATATT+TGG - chr1.2:34301710-34301729 None:intergenic 30.0%
AACAATAATCCAGACTACTT+AGG - chr1.2:34301775-34301794 None:intergenic 30.0%
ACAAACAAACAGCCATATTT+GGG - chr1.2:34301709-34301728 None:intergenic 30.0%
ACAACTTCTAGTAAATGTTG+TGG + chr1.2:34301659-34301678 MS.gene040704:intron 30.0%
ACGCGTTTACTGAAAATTAA+GGG + chr1.2:34301250-34301269 MS.gene040704:intron 30.0%
CAACTTCTAGTAAATGTTGT+GGG + chr1.2:34301660-34301679 MS.gene040704:intron 30.0%
TACGCGTTTACTGAAAATTA+AGG + chr1.2:34301249-34301268 MS.gene040704:intron 30.0%
TTGTTGAGGAAATTGAAGTT+TGG + chr1.2:34301790-34301809 MS.gene040704:intron 30.0%
!! AAATTTTAGGAGCCCAAATA+TGG + chr1.2:34301694-34301713 MS.gene040704:intron 30.0%
!! CATCTTGGTAAGTTTGTTTT+AGG + chr1.2:34300521-34300540 MS.gene040704:intron 30.0%
!! GTGTTGCTGAAAATTGTTTT+TGG + chr1.2:34301731-34301750 MS.gene040704:intron 30.0%
!! TCTTTTTGTTCTTGAGTTGT+AGG + chr1.2:34300897-34300916 MS.gene040704:intron 30.0%
!!! TTGTTTTGGAATGTTTGGTT+GGG + chr1.2:34300572-34300591 MS.gene040704:intron 30.0%
!!! TTTGTTTTGGAATGTTTGGT+TGG + chr1.2:34300571-34300590 MS.gene040704:intron 30.0%
AAGCATGGACTTACTGTTAA+CGG + chr1.2:34301534-34301553 MS.gene040704:intron 35.0%
AATAGCATATAGCCCAATAG+AGG - chr1.2:34300839-34300858 None:intergenic 35.0%
AGAGAATTTGACATAGTGAG+TGG - chr1.2:34301042-34301061 None:intergenic 35.0%
AGTAGTCTGGATTATTGTTG+AGG + chr1.2:34301776-34301795 MS.gene040704:intron 35.0%
ATAAAAACAACACACTGCAG+TGG - chr1.2:34301904-34301923 None:intergenic 35.0%
ATGAAATTCAACTAGCTGCA+AGG + chr1.2:34301478-34301497 MS.gene040704:intron 35.0%
ATGCTATTGTGAAATCCGAA+AGG + chr1.2:34300947-34300966 MS.gene040704:intron 35.0%
ATTCGTCGTGTTTGTTTGTT+TGG + chr1.2:34301116-34301135 MS.gene040704:intron 35.0%
ATTGCTGAATATTCCCCTTT+CGG - chr1.2:34300965-34300984 None:intergenic 35.0%
CACACAATCACTTAAAGAAG+TGG + chr1.2:34301188-34301207 MS.gene040704:intron 35.0%
CCAGAGATTGTAAAATGCAA+TGG - chr1.2:34301873-34301892 None:intergenic 35.0%
TCTTGATGTTGAATGTCTTG+CGG - chr1.2:34302076-34302095 None:intergenic 35.0%
TGAAATTCAACTAGCTGCAA+GGG + chr1.2:34301479-34301498 MS.gene040704:intron 35.0%
TGCTATTGTGAAATCCGAAA+GGG + chr1.2:34300948-34300967 MS.gene040704:intron 35.0%
TGGAACTATAGTGAACTGAA+TGG + chr1.2:34301420-34301439 MS.gene040704:intron 35.0%
! ACACTATCGATTAGAAGTGT+CGG + chr1.2:34301632-34301651 MS.gene040704:intron 35.0%
! CCATTGCATTTTACAATCTC+TGG + chr1.2:34301870-34301889 MS.gene040704:intron 35.0%
! TTGTACTATGTCACTTGTCA+AGG + chr1.2:34301600-34301619 MS.gene040704:intron 35.0%
!! TACGCTTTTTGATCTCATCT+TGG + chr1.2:34300506-34300525 MS.gene040704:CDS 35.0%
!!! AGGATTAGGGTTTTTGGTTT+TGG + chr1.2:34300666-34300685 MS.gene040704:intron 35.0%
!!! GGATTAGGGTTTTTGGTTTT+GGG + chr1.2:34300667-34300686 MS.gene040704:intron 35.0%
!!! GGGTTTTGTTTTGGAATGTT+TGG + chr1.2:34300567-34300586 MS.gene040704:intron 35.0%
!!! TGTTTTGGAATGTTTGGTTG+GGG + chr1.2:34300573-34300592 MS.gene040704:intron 35.0%
AAAAAGATAGCCACAAGAGC+AGG - chr1.2:34300884-34300903 None:intergenic 40.0%
AAGTTCGTAGGGAAAACCAA+TGG + chr1.2:34302123-34302142 MS.gene040704:CDS 40.0%
ACTGTTGCAGACATGATCAA+GGG + chr1.2:34302032-34302051 MS.gene040704:CDS 40.0%
AGTTCGTAGGGAAAACCAAT+GGG + chr1.2:34302124-34302143 MS.gene040704:CDS 40.0%
ATTGTGCATTGTGCCTCTAT+TGG + chr1.2:34300823-34300842 MS.gene040704:intron 40.0%
CAAGAATGATTTCTCACCTG+AGG + chr1.2:34302091-34302110 MS.gene040704:CDS 40.0%
CTTGAATATCAAGAGCTTGC+TGG + chr1.2:34301995-34302014 MS.gene040704:CDS 40.0%
GCTATTGTGAAATCCGAAAG+GGG + chr1.2:34300949-34300968 MS.gene040704:intron 40.0%
TTGTGCATTGTGCCTCTATT+GGG + chr1.2:34300824-34300843 MS.gene040704:intron 40.0%
! AAGATCTTGCTGGTAACGTT+GGG - chr1.2:34300372-34300391 None:intergenic 40.0%
! TATGTCACTTGTCAAGGTTC+TGG + chr1.2:34301606-34301625 MS.gene040704:intron 40.0%
!!! GTTTTGGAATGTTTGGTTGG+GGG + chr1.2:34300574-34300593 MS.gene040704:intron 40.0%
ACGAGGATGAGCTTAAGTCT+TGG + chr1.2:34300454-34300473 MS.gene040704:CDS 45.0%
CAACGTTACCAGCAAGATCT+TGG + chr1.2:34300371-34300390 MS.gene040704:CDS 45.0%
CGAGGATGAGCTTAAGTCTT+GGG + chr1.2:34300455-34300474 MS.gene040704:CDS 45.0%
CTGATCAACCTTGACGAACT+CGG - chr1.2:34300484-34300503 None:intergenic 45.0%
GACTGTTGCAGACATGATCA+AGG + chr1.2:34302031-34302050 MS.gene040704:CDS 45.0%
GACTTAAGCTCATCCTCGTT+AGG - chr1.2:34300453-34300472 None:intergenic 45.0%
TGATCATGTCTGCAACAGTC+TGG - chr1.2:34302031-34302050 None:intergenic 45.0%
TGATTTCTCACCTGAGGAAG+AGG + chr1.2:34302097-34302116 MS.gene040704:CDS 45.0%
TGCTGAAGACAAACCTAACG+AGG + chr1.2:34300437-34300456 MS.gene040704:CDS 45.0%
TGGTTGGGGGTCCTAAATTA+GGG + chr1.2:34300587-34300606 MS.gene040704:intron 45.0%
TGTTGAATTGCCTGCTCTTG+TGG + chr1.2:34300871-34300890 MS.gene040704:intron 45.0%
TTGGTTGGGGGTCCTAAATT+AGG + chr1.2:34300586-34300605 MS.gene040704:intron 45.0%
! ATCACTCTGAAGAGTTCCGA+CGG + chr1.2:34300252-34300271 MS.gene040704:CDS 45.0%
! CAAGATCTTGCTGGTAACGT+TGG - chr1.2:34300373-34300392 None:intergenic 45.0%
!!! TTGTTTTTATTTAATTTAAA+TGG + chr1.2:34301914-34301933 MS.gene040704:intron 5.0%
AATGTCTTGCGGATCTCCTC+TGG - chr1.2:34302065-34302084 None:intergenic 50.0%
AGGAAGAGGAGGAAGTTCGT+AGG + chr1.2:34302111-34302130 MS.gene040704:CDS 50.0%
CAACTAGCTGCAAGGGATCT+TGG + chr1.2:34301486-34301505 MS.gene040704:intron 50.0%
GATCAAGGGAAAGACACCAG+AGG + chr1.2:34302046-34302065 MS.gene040704:CDS 50.0%
GGAAGAGGAGGAAGTTCGTA+GGG + chr1.2:34302112-34302131 MS.gene040704:CDS 50.0%
TACCAGCAAGATCTTGGCGA+AGG + chr1.2:34300377-34300396 MS.gene040704:CDS 50.0%
TTGGGATTCCGAGTTCGTCA+AGG + chr1.2:34300473-34300492 MS.gene040704:CDS 50.0%
TTTCTCACCTGAGGAAGAGG+AGG + chr1.2:34302100-34302119 MS.gene040704:CDS 50.0%
! CGAGTTCGTCAAGGTTGATC+AGG + chr1.2:34300482-34300501 MS.gene040704:CDS 50.0%
! GAAGATGATTGCGCTGACAG+TGG + chr1.2:34300336-34300355 MS.gene040704:CDS 50.0%
! TGCTGGTAACGTTGGGAAGA+GGG - chr1.2:34300365-34300384 None:intergenic 50.0%
! TTGCTGGTAACGTTGGGAAG+AGG - chr1.2:34300366-34300385 None:intergenic 50.0%
AACCGCTTCATCGACCTCGA+AGG - chr1.2:34300283-34300302 None:intergenic 55.0%
CACCTTCGCCAAGATCTTGC+TGG - chr1.2:34300382-34300401 None:intergenic 55.0%
CATGTCTGCAACAGTCTGGC+AGG - chr1.2:34302027-34302046 None:intergenic 55.0%
CGAACTTCCTCCTCTTCCTC+AGG - chr1.2:34302110-34302129 None:intergenic 55.0%
GACCTTCGAGGTCGATGAAG+CGG + chr1.2:34300278-34300297 MS.gene040704:CDS 55.0%
GGTCGATGAAGCGGTTGCTT+TGG + chr1.2:34300287-34300306 MS.gene040704:CDS 55.0%
GACCTCGAAGGTCTCACCGT+CGG - chr1.2:34300271-34300290 None:intergenic 60.0%
TTCCGACGGTGAGACCTTCG+AGG + chr1.2:34300266-34300285 MS.gene040704:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 34300231 34302157 34300231 ID=MS.gene040704
chr1.2 mRNA 34300231 34302157 34300231 ID=MS.gene040704.t1;Parent=MS.gene040704
chr1.2 exon 34300231 34300527 34300231 ID=MS.gene040704.t1.exon1;Parent=MS.gene040704.t1
chr1.2 CDS 34300231 34300527 34300231 ID=cds.MS.gene040704.t1;Parent=MS.gene040704.t1
chr1.2 exon 34301984 34302157 34301984 ID=MS.gene040704.t1.exon2;Parent=MS.gene040704.t1
chr1.2 CDS 34301984 34302157 34301984 ID=cds.MS.gene040704.t1;Parent=MS.gene040704.t1
Gene Sequence

>MS.gene040704

ATGTCTTCAACAACAAGAAAGATCACTCTGAAGAGTTCCGACGGTGAGACCTTCGAGGTCGATGAAGCGGTTGCTTTGGAATCTCAGACGATCAAGCATATGATCGAAGATGATTGCGCTGACAGTGGAATCCCTCTTCCCAACGTTACCAGCAAGATCTTGGCGAAGGTGATTGAGTATTGCAAGAAGCATGTTGACGCTGCTGCTGCTGAAGACAAACCTAACGAGGATGAGCTTAAGTCTTGGGATTCCGAGTTCGTCAAGGTTGATCAGGCTACGCTTTTTGATCTCATCTTGGTAAGTTTGTTTTAGGGTTTTAATTCGTTTATTTGATTTGGGTTTTGTTTTGGAATGTTTGGTTGGGGGTCCTAAATTAGGGTTTTCTTGTTATCTGAATAGTTTATTGGTTTTCCTATTTTTTATTAATAATAATAAAGGATTAGGGTTTTTGGTTTTGGGAATTGATTGATGTTGCTTGCTGCAAATTGATCCTTGAAGTTTTTTCAATTTCAATTTGTCTTTTGTTTGGGTTGAATTTAATGGGTTATCAGTAGTTGTTTGAATTGTGATTGTTGTTGTTGATGCTGTTACAATTGTGCATTGTGCCTCTATTGGGCTATATGCTATTGTTTTGATGTTTTGTTGAATTGCCTGCTCTTGTGGCTATCTTTTTGTTCTTGAGTTGTAGGATATTGTTATTTATAGTTGATGCTGCTATGCTATTGTGAAATCCGAAAGGGGAATATTCAGCAATTTTATTTATGTTTTCATTTGGATATAATTGATATTCTTATTCTTTTTACATTAACCACTCACTATGTCAAATTCTCTGTTTTGTATGTTTATGTTATGTTTTGTTGGCTTATCTTTTCATTGTGTTCAAATATTCGTCGTGTTTGTTTGTTTGGATAACTTGCACAATGCAATGCTATTGTGTTTATTATTCAGTGTTTGTATCACACAATCACTTAAAGAAGTGGTTCTAATGTATTTGATTAAGTGGTTCTAATTTTCAGGCTACGCGTTTACTGAAAATTAAGGGCTTGTTTTTAGAAGAAGTTTCTGTTTGATATATCATATTGTTTTCGCTGTGTTTTAAGAAACAGTTGAAACAAGTTTTAAGCAAAAATATCATATTCCTTCAATGTTTTATAGAACTTTAGAAATTAGTATGATATCATTGTTTGTATGGAACTATAGTGAACTGAATGGTTCTTTTTAAGATTAAACCGAACATCAATTGATTTATGAAATTCAACTAGCTGCAAGGGATCTTGGTTTTTTTATGAATAAAGAACATATAAAGCATGGACTTACTGTTAACGGACATGTCGTCACCAACAAAATATTGAGACAATGTCATAATTAATTGTACTATGTCACTTGTCAAGGTTCTGGATCACACTATCGATTAGAAGTGTCGGTGCTACAACTTCTAGTAAATGTTGTGGGTTTATTCTTTTAAATTTTAGGAGCCCAAATATGGCTGTTTGTTTGTTTGTGTTGCTGAAAATTGTTTTTGGATTCCATTATTAAAAAAACCTAAGTAGTCTGGATTATTGTTGAGGAAATTGAAGTTTGGATTCTGAATAATTTTATAATCAATTTTGTTGTAAACTCACAAAAACGTCTCTGATTGCCATTGCATTTTACAATCTCTGGTGTTTTCTCCACTGCAGTGTGTTGTTTTTATTTAATTTAAATGGTTAAGGCTGATTTTATATTATTGGTTTGACATTTTTTTTCTTTTTAGGCTGCTAATTACTTGAATATCAAGAGCTTGCTGGATCTTACCTGCCAGACTGTTGCAGACATGATCAAGGGAAAGACACCAGAGGAGATCCGCAAGACATTCAACATCAAGAATGATTTCTCACCTGAGGAAGAGGAGGAAGTTCGTAGGGAAAACCAATGGGCATTTGAATGA
Protein sequence

>MS.gene040704.t1

MSSTTRKITLKSSDGETFEVDEAVALESQTIKHMIEDDCADSGIPLPNVTSKILAKVIEYCKKHVDAAAAEDKPNEDELKSWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFSPEEEEEVRRENQWAFE