AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33667

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33667.t1 MTR_4g013255 93.093 666 13 1 2 634 4 669 0.0 1232
MS.gene33667.t1 MTR_7g112570 37.519 645 354 12 8 611 42 678 8.94e-114 356
MS.gene33667.t1 MTR_7g112570 38.095 567 304 10 8 535 42 600 3.27e-101 321
MS.gene33667.t1 MTR_7g112570 26.471 272 166 8 193 435 337 603 1.02e-12 71.6
MS.gene33667.t1 MTR_7g112570 37.937 572 307 11 8 539 42 605 1.18e-100 320
MS.gene33667.t1 MTR_7g112570 30.857 175 116 1 402 571 104 278 6.60e-13 72.0
MS.gene33667.t1 MTR_2g021030 28.976 459 310 9 139 583 127 583 4.02e-40 155
MS.gene33667.t1 MTR_2g021030 26.496 468 276 14 58 512 176 588 4.51e-28 119
MS.gene33667.t1 MTR_2g021030 27.419 310 190 9 311 589 127 432 1.36e-20 96.7
MS.gene33667.t1 MTR_2g021030 28.221 326 179 7 220 516 138 437 3.02e-29 121
MS.gene33667.t1 MTR_2g021030 27.976 336 208 5 139 465 127 437 9.65e-24 105
MS.gene33667.t1 MTR_2g021030 27.742 310 190 7 285 564 127 432 5.86e-23 102
MS.gene33667.t1 MTR_1g114610 28.536 403 243 14 13 394 18 396 1.04e-28 119
MS.gene33667.t1 MTR_1g114610 28.947 266 161 5 285 546 95 336 2.18e-22 100
MS.gene33667.t1 MTR_1g114610 28.148 270 161 4 285 521 121 390 5.47e-20 93.2
MS.gene33667.t1 MTR_1g012520 27.764 407 239 11 261 632 154 540 9.01e-27 115
MS.gene33667.t1 MTR_1g012520 27.642 492 301 20 85 549 143 606 2.34e-26 114
MS.gene33667.t1 MTR_1g012520 23.636 440 265 12 75 470 217 629 3.06e-14 76.6
MS.gene33667.t1 MTR_4g009030 24.000 550 345 14 34 534 22 547 1.80e-24 108
MS.gene33667.t1 MTR_8g017160 24.771 545 354 13 31 536 48 575 3.07e-23 104
MS.gene33667.t1 MTR_3g107280 25.168 596 357 21 6 564 62 605 3.04e-22 101
MS.gene33667.t1 MTR_3g107280 25.153 326 209 8 285 575 174 499 5.15e-19 91.7
MS.gene33667.t1 MTR_3g107280 28.188 298 185 12 77 366 349 625 4.62e-11 66.2
MS.gene33667.t1 MTR_8g037780 24.188 554 343 14 32 536 44 569 3.10e-22 101
MS.gene33667.t1 MTR_3g088895 30.769 208 117 3 271 475 99 282 2.33e-20 93.6
MS.gene33667.t1 MTR_3g088895 32.500 160 104 4 371 526 123 282 6.00e-14 74.3
MS.gene33667.t1 MTR_3g088895 25.806 217 135 4 285 483 141 349 1.36e-11 67.0
MS.gene33667.t1 MTR_1g054625 26.299 308 182 8 189 481 74 351 2.76e-20 93.2
MS.gene33667.t1 MTR_1g054625 28.458 253 172 6 320 565 75 325 6.42e-17 83.2
MS.gene33667.t1 MTR_1g029500 30.328 244 142 4 236 475 50 269 5.76e-20 92.4
MS.gene33667.t1 MTR_1g029500 30.515 272 172 8 314 580 58 317 2.83e-18 87.4
MS.gene33667.t1 MTR_1g029500 26.923 364 173 11 102 462 64 337 3.37e-11 65.9
MS.gene33667.t1 MTR_1g054625 27.381 252 150 5 223 469 13 236 1.81e-19 89.0
MS.gene33667.t1 MTR_1g054625 31.193 218 145 5 351 565 13 228 1.00e-14 75.1
MS.gene33667.t1 MTR_4g019320 24.231 553 360 16 12 534 13 536 3.53e-19 92.0
MS.gene33667.t1 MTR_4g019320 27.230 426 261 12 75 477 159 558 2.07e-18 89.4
MS.gene33667.t1 MTR_6g016905 24.408 549 302 20 33 510 31 537 1.58e-18 89.7
MS.gene33667.t1 MTR_4g006450 25.442 566 331 18 18 534 14 537 5.31e-18 88.2
MS.gene33667.t1 MTR_8g028140 26.557 305 169 10 192 470 13 288 1.27e-17 84.7
MS.gene33667.t1 MTR_8g028140 25.000 252 159 7 300 524 17 265 1.62e-15 78.6
MS.gene33667.t1 MTR_8g028140 30.800 250 140 13 367 588 7 251 2.50e-14 74.7
MS.gene33667.t1 MTR_3g110740 24.395 537 317 17 28 509 62 564 1.87e-17 86.7
MS.gene33667.t1 MTR_5g095830 24.905 526 308 20 73 534 81 583 3.18e-17 85.9
MS.gene33667.t1 MTR_5g095830 27.090 299 204 6 170 460 294 586 3.79e-11 66.6
MS.gene33667.t1 MTR_5g088250 32.990 194 120 4 287 477 359 545 4.17e-17 85.1
MS.gene33667.t1 MTR_4g114640 29.499 339 202 11 284 586 166 503 4.54e-17 85.5
MS.gene33667.t1 MTR_4g114640 24.731 465 268 15 110 550 164 570 2.45e-16 83.2
MS.gene33667.t1 MTR_8g028140 26.557 305 169 10 192 470 93 368 4.79e-17 84.0
MS.gene33667.t1 MTR_8g028140 30.738 244 137 12 373 588 92 331 4.28e-14 75.1
MS.gene33667.t1 MTR_8g028140 24.324 296 165 8 300 567 97 361 4.48e-14 75.1
MS.gene33667.t1 MTR_3g408430 28.516 256 172 6 286 534 198 449 1.52e-16 83.2
MS.gene33667.t1 MTR_5g075860 25.197 381 256 10 100 460 174 545 1.63e-16 83.6
MS.gene33667.t1 MTR_5g075860 21.277 564 356 16 23 534 15 542 6.05e-16 81.6
MS.gene33667.t1 MTR_3g465770 21.703 364 246 7 208 534 198 559 1.79e-16 83.6
MS.gene33667.t1 MTR_4g133640 24.014 583 378 19 18 558 40 599 2.29e-16 83.2
MS.gene33667.t1 MTR_3g006050 25.494 557 330 20 32 534 51 576 1.76e-15 80.5
MS.gene33667.t1 MTR_3g006050 26.994 326 207 8 172 477 288 602 6.41e-12 68.9
MS.gene33667.t1 MTR_1g015450 26.538 569 309 22 102 607 342 864 2.11e-15 80.5
MS.gene33667.t1 MTR_3g074550 26.644 289 168 6 208 460 91 371 2.27e-15 78.6
MS.gene33667.t1 MTR_4g094932 28.235 255 168 6 307 550 116 366 3.12e-15 79.3
MS.gene33667.t1 MTR_4g094932 28.406 345 222 14 221 546 132 470 3.72e-15 79.0
MS.gene33667.t1 MTR_4g094932 25.367 477 268 20 6 469 37 438 6.05e-12 68.9
MS.gene33667.t1 MTR_4g006240 24.505 555 340 19 32 534 25 552 3.54e-15 79.3
MS.gene33667.t1 MTR_4g006240 27.303 304 198 8 170 460 262 555 1.26e-14 77.4
MS.gene33667.t1 MTR_4g036815 27.386 241 165 4 289 523 216 452 5.55e-15 78.6
MS.gene33667.t1 MTR_2g007590 24.275 552 367 18 18 535 40 574 3.09e-14 76.3
MS.gene33667.t1 MTR_4g125410 29.225 284 176 10 193 457 161 438 3.38e-14 75.9
MS.gene33667.t1 MTR_3g036270 24.304 539 307 17 34 505 92 596 5.39e-14 75.5
MS.gene33667.t1 MTR_7g029535 24.819 552 348 23 2 505 62 594 7.16e-14 75.1
MS.gene33667.t1 MTR_6g016725 25.151 497 285 18 68 505 128 596 8.40e-14 75.1
MS.gene33667.t1 MTR_1g018790 29.675 246 165 5 311 550 138 381 9.47e-14 74.7
MS.gene33667.t1 MTR_1g018790 26.087 253 182 4 283 531 187 438 2.87e-13 73.2
MS.gene33667.t1 MTR_4g059340 23.783 534 360 12 28 543 26 530 4.99e-13 72.4
MS.gene33667.t1 MTR_4g021765 24.916 297 208 7 188 477 45 333 6.04e-13 70.9
MS.gene33667.t1 MTR_1g050478 22.719 537 321 18 30 505 38 541 1.14e-12 71.2
MS.gene33667.t1 MTR_8g045150 24.056 503 307 18 23 476 17 493 1.15e-12 71.2
MS.gene33667.t1 MTR_4g024200 24.405 336 201 9 232 534 136 451 1.88e-12 70.5
MS.gene33667.t1 MTR_4g021835 26.538 260 165 8 289 534 193 440 3.41e-12 69.3
MS.gene33667.t1 MTR_3g083280 24.375 480 304 13 32 461 23 493 5.88e-12 68.9
MS.gene33667.t1 MTR_6g016705 24.186 430 267 14 31 441 13 402 8.38e-12 68.2
MS.gene33667.t1 MTR_4g114650 25.000 352 189 8 206 524 202 511 1.70e-11 67.8
MS.gene33667.t1 MTR_4g114650 23.864 440 289 14 130 537 179 604 2.24e-11 67.4
MS.gene33667.t1 MTR_4g114650 26.667 375 246 14 196 549 224 590 6.76e-11 65.9
MS.gene33667.t1 MTR_4g114650 23.864 440 289 14 130 537 124 549 1.88e-11 67.8
MS.gene33667.t1 MTR_4g114650 26.667 375 246 14 196 549 169 535 8.24e-11 65.5
MS.gene33667.t1 MTR_4g116170 26.829 451 293 21 121 560 125 549 2.02e-11 67.4
MS.gene33667.t1 MTR_4g114650 23.864 440 289 14 130 537 128 553 2.24e-11 67.4
MS.gene33667.t1 MTR_4g114650 26.667 375 246 14 196 549 173 539 9.57e-11 65.5
MS.gene33667.t1 MTR_3g081260 25.877 228 148 5 250 460 11 234 3.05e-11 64.7
MS.gene33667.t1 MTR_8g076010 23.894 452 291 12 32 477 37 441 3.13e-11 66.2
MS.gene33667.t1 MTR_4g008330 31.169 154 98 4 402 551 9 158 4.00e-11 62.4
MS.gene33667.t1 MTR_6g025290 22.581 310 202 6 233 513 112 412 4.25e-11 65.9
MS.gene33667.t1 MTR_4g435338 29.221 154 103 4 383 534 150 299 5.16e-11 64.7
MS.gene33667.t1 MTR_1g023530 32.414 145 95 2 336 477 310 454 5.27e-11 65.9
MS.gene33667.t1 MTR_4g006290 29.008 262 145 12 296 538 209 448 7.62e-11 65.1
MS.gene33667.t1 MTR_6g054950 26.316 228 117 7 300 526 192 369 9.33e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33667.t1 AT5G01720 58.321 667 239 5 1 632 1 663 0.0 659
MS.gene33667.t1 AT5G27920 37.383 642 352 14 12 611 8 641 1.21e-96 310
MS.gene33667.t1 AT4G15475 26.562 448 317 8 146 583 138 583 3.10e-34 138
MS.gene33667.t1 AT4G15475 26.609 466 315 11 65 512 132 588 8.32e-33 134
MS.gene33667.t1 AT4G15475 22.078 385 220 7 284 588 126 510 4.53e-20 95.1
MS.gene33667.t1 AT4G15475 25.733 307 173 8 337 589 127 432 8.91e-17 84.3
MS.gene33667.t1 AT2G25490 28.982 452 280 13 117 552 168 594 2.92e-28 120
MS.gene33667.t1 AT2G25490 29.446 343 194 13 269 571 151 485 3.52e-23 104
MS.gene33667.t1 AT2G25490 24.545 440 278 12 102 519 205 612 4.11e-18 89.0
MS.gene33667.t1 AT1G21410 32.558 215 121 3 261 475 81 271 3.30e-22 99.4
MS.gene33667.t1 AT1G21410 30.804 224 143 7 308 526 55 271 6.05e-18 86.3
MS.gene33667.t1 AT1G21410 36.170 141 87 3 413 550 104 244 3.96e-16 80.9
MS.gene33667.t1 AT1G21410 29.181 281 155 10 137 403 56 306 3.07e-13 72.0
MS.gene33667.t1 AT1G21410 32.558 215 121 3 261 475 81 271 3.30e-22 99.4
MS.gene33667.t1 AT1G21410 30.804 224 143 7 308 526 55 271 6.05e-18 86.3
MS.gene33667.t1 AT1G21410 36.170 141 87 3 413 550 104 244 3.96e-16 80.9
MS.gene33667.t1 AT1G21410 29.181 281 155 10 137 403 56 306 3.07e-13 72.0
MS.gene33667.t1 AT1G77000 32.850 207 114 4 271 475 88 271 1.27e-21 97.4
MS.gene33667.t1 AT1G77000 31.696 224 141 7 308 526 55 271 1.46e-18 88.2
MS.gene33667.t1 AT1G77000 35.461 141 88 3 413 550 104 244 1.14e-14 76.6
MS.gene33667.t1 AT1G77000 29.044 272 143 9 194 460 117 343 3.80e-14 74.7
MS.gene33667.t1 AT1G77000 32.850 207 114 4 271 475 88 271 1.27e-21 97.4
MS.gene33667.t1 AT1G77000 31.696 224 141 7 308 526 55 271 1.46e-18 88.2
MS.gene33667.t1 AT1G77000 35.461 141 88 3 413 550 104 244 1.14e-14 76.6
MS.gene33667.t1 AT1G77000 29.044 272 143 9 194 460 117 343 3.80e-14 74.7
MS.gene33667.t1 AT3G58530 27.241 290 172 7 189 469 76 335 2.87e-21 96.3
MS.gene33667.t1 AT3G58530 25.899 278 191 8 296 565 57 327 9.04e-14 73.6
MS.gene33667.t1 AT5G23340 30.147 272 157 7 284 547 110 356 5.08e-21 96.3
MS.gene33667.t1 AT5G23340 25.702 463 250 15 13 458 8 393 8.41e-21 95.5
MS.gene33667.t1 AT5G23340 40.000 125 74 1 402 525 100 224 8.40e-19 89.7
MS.gene33667.t1 AT5G23340 27.586 174 122 3 412 581 84 257 1.13e-13 73.9
MS.gene33667.t1 AT5G25350 27.160 324 198 7 285 571 154 476 7.57e-21 97.4
MS.gene33667.t1 AT5G25350 24.508 457 289 15 75 519 195 607 4.88e-19 91.7
MS.gene33667.t1 AT5G25350 23.206 418 227 12 219 543 164 580 6.45e-17 85.1
MS.gene33667.t1 AT5G25350 29.108 213 132 6 413 609 154 363 8.58e-15 78.2
MS.gene33667.t1 AT5G51370 28.629 248 163 6 284 523 160 401 2.52e-17 85.5
MS.gene33667.t1 AT1G80630 30.233 258 147 8 306 534 275 528 1.19e-16 84.0
MS.gene33667.t1 AT5G51380 28.689 244 160 6 284 519 196 433 3.10e-16 82.4
MS.gene33667.t1 AT1G47056 24.925 333 218 7 207 536 117 420 4.21e-16 82.0
MS.gene33667.t1 AT1G47056 30.612 245 162 6 311 550 117 358 2.54e-15 79.7
MS.gene33667.t1 AT1G80570 25.049 507 283 17 12 461 1 467 9.25e-16 80.9
MS.gene33667.t1 AT1G80570 26.009 223 128 2 363 548 84 306 2.59e-11 66.6
MS.gene33667.t1 AT1G80570 25.049 507 283 17 12 461 1 467 9.25e-16 80.9
MS.gene33667.t1 AT1G80570 26.009 223 128 2 363 548 84 306 2.59e-11 66.6
MS.gene33667.t1 AT1G80570 25.049 507 283 17 12 461 14 480 1.08e-15 80.5
MS.gene33667.t1 AT1G80570 26.009 223 128 2 363 548 97 319 3.33e-11 66.6
MS.gene33667.t1 AT3G07550 28.165 316 212 10 195 505 17 322 2.44e-15 78.6
MS.gene33667.t1 AT3G07550 25.979 281 196 9 182 457 52 325 8.07e-11 64.7
MS.gene33667.t1 AT5G07670 26.721 247 168 5 284 523 194 434 4.56e-15 78.6
MS.gene33667.t1 AT1G55590 26.531 245 165 6 285 516 298 540 2.85e-14 76.6
MS.gene33667.t1 AT1G55590 27.737 274 156 11 216 475 305 550 3.72e-11 66.6
MS.gene33667.t1 AT5G67250 28.315 279 190 6 280 550 86 362 2.91e-14 76.3
MS.gene33667.t1 AT4G07400 24.611 321 207 7 221 536 163 453 3.21e-14 76.3
MS.gene33667.t1 AT4G07400 27.801 241 167 5 310 546 150 387 3.72e-12 69.7
MS.gene33667.t1 AT1G77000 35.714 126 79 2 413 536 104 229 3.26e-14 73.6
MS.gene33667.t1 AT1G77000 31.217 189 118 7 308 491 55 236 2.26e-13 71.2
MS.gene33667.t1 AT1G77000 31.977 172 92 4 271 440 88 236 3.88e-13 70.5
MS.gene33667.t1 AT1G77000 35.714 126 79 2 413 536 104 229 3.26e-14 73.6
MS.gene33667.t1 AT1G77000 31.217 189 118 7 308 491 55 236 2.26e-13 71.2
MS.gene33667.t1 AT1G77000 31.977 172 92 4 271 440 88 236 3.88e-13 70.5
MS.gene33667.t1 AT3G50080 28.980 245 163 5 311 550 118 356 5.30e-14 75.5
MS.gene33667.t1 AT3G07550 26.012 346 236 10 174 505 10 349 5.72e-14 74.7
MS.gene33667.t1 AT3G07550 26.012 346 236 10 174 505 10 349 5.72e-14 74.7
MS.gene33667.t1 AT4G33210 24.202 595 339 23 63 565 246 820 7.79e-13 72.4
MS.gene33667.t1 AT2G39940 23.518 523 306 22 17 487 18 498 3.98e-11 66.6
MS.gene33667.t1 AT5G51370 28.796 191 124 4 284 467 160 345 4.83e-11 65.5

Find 118 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATACCTGTCTTAGATTT+TGG 0.092000 4.3:+84302263 None:intergenic
ATGGAGAGATAAAGAGTTTC+AGG 0.182908 4.3:-84301922 MS.gene33667:intron
TGGCAAAACACAAAGATTTA+AGG 0.193185 4.3:-84303236 MS.gene33667:CDS
TCAGGCAGATTCCCAGTTTC+AGG 0.217412 4.3:+84302983 None:intergenic
AGTCGATATCTCGTTGTTCT+AGG 0.233081 4.3:-84303014 MS.gene33667:CDS
ACGCTCCAGCGATTAAATTA+AGG 0.235182 4.3:+84305248 None:intergenic
TGAAATCTTCTGTTTCTTCA+TGG 0.235708 4.3:+84305519 None:intergenic
TAAAGGAACTGGATCTTTAC+AGG 0.239285 4.3:-84302912 MS.gene33667:intron
TCAAAGCTAACGAGCATTTC+TGG 0.240254 4.3:-84303564 MS.gene33667:CDS
ACAAAGTTCTTTACATTTGA+TGG 0.250302 4.3:+84304922 None:intergenic
GGAATCTCAGCAATTGCTTC+TGG 0.272590 4.3:-84302520 MS.gene33667:CDS
TGCTGTGAGATGTGTTAATT+TGG 0.288386 4.3:-84305155 MS.gene33667:CDS
TGTTGCACGCGCTGTGAATT+TGG 0.288731 4.3:-84305080 MS.gene33667:CDS
AGGACTTGCAGCGGCCTTAT+TGG 0.299023 4.3:-84302045 MS.gene33667:CDS
TAGGCTCTCCAGCCTGAAAC+TGG 0.312832 4.3:-84302995 MS.gene33667:CDS
ACAAGGGTGCAAGACATTAA+AGG 0.329237 4.3:-84303843 MS.gene33667:intron
ATTGCAACATCTCGAAGATT+TGG 0.329725 4.3:-84303921 MS.gene33667:CDS
TGGAGAGGCTTTGGTTGAAT+AGG 0.334167 4.3:-84305060 MS.gene33667:CDS
CATGTGTTGCTCAAAGTTAA+AGG 0.334579 4.3:-84302929 MS.gene33667:CDS
GTTAGAAGATCTGATGCATT+AGG 0.341678 4.3:-84301746 None:intergenic
TTTGGATCTCTCTTATTTGC+CGG 0.350069 4.3:-84304897 MS.gene33667:intron
GTCCCTTCGACAGGCTGAAT+TGG 0.355973 4.3:-84301791 MS.gene33667:intron
TGTCGAACGCGACGGAGTTA+AGG 0.360260 4.3:-84305120 MS.gene33667:CDS
ATTGCACTAGCATTACTGAT+AGG 0.363613 4.3:-84302465 MS.gene33667:CDS
TGCCTGAACATAACTGATAA+AGG 0.366531 4.3:-84302967 MS.gene33667:CDS
CTCGAAGATTTGGTCCTTGA+AGG 0.368569 4.3:-84303911 MS.gene33667:CDS
TCGGAAGCGTATATATTGAT+TGG 0.373142 4.3:-84303102 MS.gene33667:CDS
GTCGAACGCGACGGAGTTAA+GGG 0.373469 4.3:-84305119 MS.gene33667:CDS
CCACGCCCTGCAGGTGAAGC+AGG 0.375732 4.3:+84302075 None:intergenic
AAGGTTTGTTCGTTGTGTTT+TGG 0.376482 4.3:+84305433 None:intergenic
GTAGGGCTTCTGTCGCTTGC+CGG 0.382619 4.3:-84302122 MS.gene33667:CDS
CGCTCCAGCGATTAAATTAA+GGG 0.383197 4.3:+84305249 None:intergenic
CTCCTTTATCAGTTATGTTC+AGG 0.387683 4.3:+84302965 None:intergenic
TTGGTCCTTGAAGGATGCTT+TGG 0.388122 4.3:-84303902 MS.gene33667:CDS
TCTTGTTACATCGTTTGGTT+TGG 0.396585 4.3:-84302383 MS.gene33667:CDS
TTGCTCAAAGTTAAAGGAAC+TGG 0.397366 4.3:-84302923 MS.gene33667:CDS
TGCACAGGAGTAACAGATTT+GGG 0.398167 4.3:-84302541 MS.gene33667:CDS
TAGCCCTCCGCATGCCAATA+AGG 0.398609 4.3:+84302031 None:intergenic
GATCAAGCTCTTCAAGATAA+TGG 0.398688 4.3:+84303070 None:intergenic
GTGCACAGGAGTAACAGATT+TGG 0.401289 4.3:-84302542 MS.gene33667:CDS
CTGTGAATTTGGAGAGGCTT+TGG 0.407431 4.3:-84305069 MS.gene33667:CDS
ATGTAAAGAACTTTGTACTT+TGG 0.413175 4.3:-84304915 MS.gene33667:CDS
CTTGTGGAGCCACGCCCTGC+AGG 0.414350 4.3:+84302066 None:intergenic
TGTCAGAACATAAGTCATAT+TGG 0.416282 4.3:-84303591 MS.gene33667:CDS
TTGTATGTGTTCGGCGCGTA+AGG 0.417047 4.3:+84305342 None:intergenic
CTTTATCTCTCCATTCAAAC+AGG 0.418740 4.3:+84301931 None:intergenic
GTGAAAATATTTGCACGTTA+AGG 0.435010 4.3:+84305399 None:intergenic
CTTGAAGGATGCTTTGGCAT+TGG 0.446649 4.3:-84303896 MS.gene33667:CDS
GAGAAGTGGTTGTATGTGTT+CGG 0.447579 4.3:+84305333 None:intergenic
TTTCACCCTGCTTCACCTGC+AGG 0.448974 4.3:-84302081 MS.gene33667:CDS
CTGCAGAAACTTGATATCTC+AGG 0.449246 4.3:-84303615 MS.gene33667:intron
CGTTAAGGAGAATGATTTCA+AGG 0.458815 4.3:+84305414 None:intergenic
GATCGACGAGAGACATTTCT+CGG 0.463678 4.3:+84303949 None:intergenic
CTAAGCTTAAGCAAATGTTC+AGG 0.466812 4.3:-84303288 MS.gene33667:CDS
GCGGCCTTATTGGCATGCGG+AGG 0.468002 4.3:-84302035 MS.gene33667:CDS
GTCATTCACGCGCGGACATA+AGG 0.469405 4.3:+84305276 None:intergenic
TTGCAGACACTTGAAATTCG+AGG 0.470964 4.3:-84302412 MS.gene33667:CDS
CCTGCTTCACCTGCAGGGCG+TGG 0.471045 4.3:-84302075 MS.gene33667:CDS
TATACCTCAGTTACAGATGT+AGG 0.471322 4.3:-84302140 MS.gene33667:CDS
TCTTCTGAGAGTGTTTCAAA+TGG 0.472701 4.3:+84305487 None:intergenic
CTTCTGAGAGTGTTTCAAAT+GGG 0.472728 4.3:+84305488 None:intergenic
CACGTGGATGCCTGTTTGAA+TGG 0.481933 4.3:-84301941 MS.gene33667:CDS
CAATGCCAAAGCATCCTTCA+AGG 0.482251 4.3:+84303897 None:intergenic
TACATCTGTAACTGAGGTAT+AGG 0.484311 4.3:+84302142 None:intergenic
AACTGTAATTTCCAACACCT+TGG 0.486142 4.3:+84301766 None:intergenic
ACATGTCTGATGAGAAGCTT+GGG 0.488138 4.3:+84301969 None:intergenic
GCATTAATCATCTGAAGGCT+AGG 0.491551 4.3:+84302493 None:intergenic
ACTGTAATTTCCAACACCTT+GGG 0.493152 4.3:+84301767 None:intergenic
GGAGTTAAGGGATGCTGCTA+TGG 0.495204 4.3:-84305107 MS.gene33667:CDS
GATTGAATTTATCGCGGTCG+AGG 0.497881 4.3:-84305207 MS.gene33667:CDS
ACGTCCCTTAATTTAATCGC+TGG 0.501745 4.3:-84305253 MS.gene33667:CDS
TACATGTCTGATGAGAAGCT+TGG 0.503041 4.3:+84301968 None:intergenic
CGGCCTTATTGGCATGCGGA+GGG 0.504363 4.3:-84302034 MS.gene33667:CDS
GTCGTGTACGCTAGTTTCAT+CGG 0.507526 4.3:-84303121 MS.gene33667:CDS
GTTTGAAGACTTCGTTATTG+AGG 0.507878 4.3:+84303864 None:intergenic
TATATTAGCAGATGGTTCTC+CGG 0.509322 4.3:-84303526 MS.gene33667:intron
GATAATGAACTTGATGATGA+AGG 0.510920 4.3:-84303042 MS.gene33667:CDS
TGTATGTGTTCGGCGCGTAA+GGG 0.516746 4.3:+84305343 None:intergenic
TTGCACTAGCATTACTGATA+GGG 0.518967 4.3:-84302464 MS.gene33667:CDS
TGTTATAACATCGATGATAG+TGG 0.520708 4.3:-84302310 MS.gene33667:CDS
ACCGCGATAAATTCAATCGC+TGG 0.523482 4.3:+84305212 None:intergenic
CTGAATTGGACCCAAGGTGT+TGG 0.525720 4.3:-84301777 MS.gene33667:CDS
AAGTCTCACTTCTCTCAAGA+TGG 0.526061 4.3:-84303145 MS.gene33667:CDS
CACGCGCTGTGAATTTGGAG+AGG 0.534580 4.3:-84305075 MS.gene33667:CDS
TGAGCAACACATGCCAACAT+TGG 0.538279 4.3:+84302939 None:intergenic
ATACCTCAGTTACAGATGTA+GGG 0.538570 4.3:-84302139 MS.gene33667:CDS
GCAGATTCCCAGTTTCAGGC+TGG 0.540372 4.3:+84302987 None:intergenic
TTCACCCTGCTTCACCTGCA+GGG 0.548706 4.3:-84302080 MS.gene33667:CDS
TTTCTCCCAAAATCTAAGAC+AGG 0.558951 4.3:-84302269 MS.gene33667:intron
CTGCAGGGCGTGGCTCCACA+AGG 0.559226 4.3:-84302065 MS.gene33667:CDS
TCTGACGGATTAAAAGCCAT+TGG 0.560221 4.3:-84303336 MS.gene33667:CDS
TTAAGGGATGCTGCTATGGT+TGG 0.564397 4.3:-84305103 MS.gene33667:CDS
TAACATCGATGATAGTGGTA+TGG 0.570088 4.3:-84302305 MS.gene33667:CDS
GCAGCGGCCTTATTGGCATG+CGG 0.573670 4.3:-84302038 MS.gene33667:CDS
TGAAATGCACAAATTTCCAA+TGG 0.577688 4.3:+84303320 None:intergenic
CACGCCCTGCAGGTGAAGCA+GGG 0.580439 4.3:+84302076 None:intergenic
GACAGGCTGAATTGGACCCA+AGG 0.581823 4.3:-84301783 MS.gene33667:intron
GAACAACTTATATTAGCAGA+TGG 0.583441 4.3:-84303534 MS.gene33667:CDS
GATAAAGGAGTTGCCAATGT+TGG 0.584204 4.3:-84302952 MS.gene33667:CDS
CATTAATCATCTGAAGGCTA+GGG 0.585423 4.3:+84302494 None:intergenic
AAGGCCGCTGCAAGTCCTTG+TGG 0.589562 4.3:+84302050 None:intergenic
GCTTGATTTGTCGAACGCGA+CGG 0.593362 4.3:-84305128 MS.gene33667:CDS
TGACGCTCTCTCTTTCCTTG+TGG 0.594714 4.3:-84303256 MS.gene33667:CDS
TTGGTGTAACGTTTGAGAAG+TGG 0.594772 4.3:+84305319 None:intergenic
GGCTGCAATATTACCTCTGA+CGG 0.601294 4.3:-84303351 MS.gene33667:CDS
AATAACGAAGTCTTCAAACA+AGG 0.602466 4.3:-84303860 MS.gene33667:CDS
AAGCAGCATTAATCATCTGA+AGG 0.604909 4.3:+84302488 None:intergenic
AAGCCCTACATCTGTAACTG+AGG 0.606396 4.3:+84302136 None:intergenic
AGGCTCTCCAGCCTGAAACT+GGG 0.607106 4.3:-84302994 MS.gene33667:CDS
TCGAAACATCGGTTATCTTG+CGG 0.627486 4.3:+84303193 None:intergenic
GGCTCCACAAGGACTTGCAG+CGG 0.629492 4.3:-84302054 MS.gene33667:CDS
ATAACGAAGTCTTCAAACAA+GGG 0.639862 4.3:-84303859 MS.gene33667:CDS
AACAACGAGATATCGACTCA+AGG 0.657950 4.3:+84303019 None:intergenic
AATGCTCGCAATCGAAACAT+CGG 0.683913 4.3:+84303182 None:intergenic
GTAAGGGACACAGAGCGCGT+CGG 0.696757 4.3:+84305359 None:intergenic
ATCAGACATGTAGAAGCACG+TGG 0.701944 4.3:-84301957 MS.gene33667:CDS
TCCAGCGATTGAATTTATCG+CGG 0.705918 4.3:-84305213 MS.gene33667:CDS
AGGGACGTGTCATTCACGCG+CGG 0.746770 4.3:+84305268 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAACAAAAGATAAAATA+AGG - chr4.3:84304484-84304503 MS.gene33667:intron 10.0%
!!! AAAAAAAAATTATTTTTTGG+TGG - chr4.3:84302611-84302630 MS.gene33667:intron 10.0%
!!! CTTTTTTGTTTTTTATTTTA+GGG - chr4.3:84302820-84302839 MS.gene33667:intron 10.0%
!!! TTTAGATTTTTACTAATTTA+TGG + chr4.3:84302992-84303011 None:intergenic 10.0%
!! CCTAAAATAAAAAACAAAAA+AGG + chr4.3:84302822-84302841 None:intergenic 15.0%
!!! AAAGTAGATTTTACAAAATA+TGG - chr4.3:84304603-84304622 MS.gene33667:intron 15.0%
!!! ATCATTTTTCTTTACAAAAT+TGG - chr4.3:84303033-84303052 MS.gene33667:CDS 15.0%
!!! ATTCAATTATAACTATTTCT+TGG - chr4.3:84303811-84303830 MS.gene33667:intron 15.0%
!!! ATTGTGAAAATATTAAGTTT+TGG - chr4.3:84303446-84303465 MS.gene33667:intron 15.0%
!!! CCTTTTTTGTTTTTTATTTT+AGG - chr4.3:84302819-84302838 MS.gene33667:intron 15.0%
!!! TACAACAAATTTAACTTTTT+TGG + chr4.3:84303488-84303507 None:intergenic 15.0%
!!! TGGTTTTAATTTTCTAAATT+TGG - chr4.3:84302696-84302715 MS.gene33667:intron 15.0%
!!! TTTTAATTTTCTAAATTTGG+AGG - chr4.3:84302699-84302718 MS.gene33667:intron 15.0%
!! AATAAAATGCGAAAAAAATG+AGG + chr4.3:84305433-84305452 None:intergenic 20.0%
!! AATTTAGATTATGAAACTGT+AGG - chr4.3:84302950-84302969 MS.gene33667:CDS 20.0%
!! ATTTAGATTATGAAACTGTA+GGG - chr4.3:84302951-84302970 MS.gene33667:CDS 20.0%
!! ATTTATGTCAACATCTAATA+AGG + chr4.3:84304656-84304675 None:intergenic 20.0%
!! TAATCTTAATTAGTTGACAT+TGG + chr4.3:84304687-84304706 None:intergenic 20.0%
!! TGTAAAATCTACTTTAGATA+AGG + chr4.3:84304598-84304617 None:intergenic 20.0%
!!! AACAAATTTAACTTTTTTGG+CGG + chr4.3:84303485-84303504 None:intergenic 20.0%
!!! AAGATTATTTTTGTCTTTTC+AGG - chr4.3:84304700-84304719 MS.gene33667:intron 20.0%
!!! ATGGTATTTAATGTTTTTGT+AGG - chr4.3:84302768-84302787 MS.gene33667:intron 20.0%
!!! ATTATTTTTTGGTGGATTAT+TGG - chr4.3:84302619-84302638 MS.gene33667:intron 20.0%
!!! CATTTTTTTCGCATTTTATT+TGG - chr4.3:84305433-84305452 MS.gene33667:CDS 20.0%
!!! GATAAATAAAGTTGATAATC+TGG - chr4.3:84302421-84302440 MS.gene33667:CDS 20.0%
!!! TAAATTTGGAGGTTTTTTAA+GGG - chr4.3:84302710-84302729 MS.gene33667:intron 20.0%
!!! TAATTAAATTTTTGCACTTG+AGG - chr4.3:84303781-84303800 MS.gene33667:intron 20.0%
!!! TATCAACTTTATTTATCCAT+TGG + chr4.3:84302418-84302437 None:intergenic 20.0%
!!! TCAATTTTAAGGTGTTTTTT+TGG - chr4.3:84304409-84304428 MS.gene33667:intron 20.0%
!!! TTAAGTGGATTATTATGAAA+TGG - chr4.3:84302896-84302915 MS.gene33667:intron 20.0%
!!! TTAATTGTTTTGAAATGACA+TGG - chr4.3:84303249-84303268 MS.gene33667:CDS 20.0%
! AAAAAATTGTGCAAATGTGA+AGG + chr4.3:84303590-84303609 None:intergenic 25.0%
! AAAATTGACAAGAAAAGCAA+AGG + chr4.3:84304397-84304416 None:intergenic 25.0%
! ACAAAGTTCTTTACATTTGA+TGG + chr4.3:84302345-84302364 None:intergenic 25.0%
! AGGAAACAAATTTCTCAATT+TGG + chr4.3:84302802-84302821 None:intergenic 25.0%
! ATGTAAAGAACTTTGTACTT+TGG - chr4.3:84302349-84302368 MS.gene33667:CDS 25.0%
! TATAAAGACTGAAGAATTAC+CGG + chr4.3:84303760-84303779 None:intergenic 25.0%
! TGACTAATGATTGTTTACAT+CGG - chr4.3:84302470-84302489 MS.gene33667:CDS 25.0%
! TGATATGTAATAAGTCATTG+TGG - chr4.3:84302676-84302695 MS.gene33667:intron 25.0%
! TTGATTATGTTCAGTAGATT+TGG - chr4.3:84303179-84303198 MS.gene33667:CDS 25.0%
! TTTAGATTATGAAACTGTAG+GGG - chr4.3:84302952-84302971 MS.gene33667:CDS 25.0%
!! AAAATACCTGTCTTAGATTT+TGG + chr4.3:84305004-84305023 None:intergenic 25.0%
!! AAATACCTGTCTTAGATTTT+GGG + chr4.3:84305003-84305022 None:intergenic 25.0%
!! GTCAGACTTTATTTATCTTT+CGG + chr4.3:84302454-84302473 None:intergenic 25.0%
!! GTTATAAAGTTCAGTAGTTT+TGG - chr4.3:84303201-84303220 MS.gene33667:CDS 25.0%
!! TCATAAAATGTGTTGCTAAA+TGG + chr4.3:84305394-84305413 None:intergenic 25.0%
!! TGCTTTTCTTGTCAATTTTA+AGG - chr4.3:84304398-84304417 MS.gene33667:intron 25.0%
!!! CGATGTTATAACACTTTTTT+AGG + chr4.3:84304947-84304966 None:intergenic 25.0%
!!! CTAAATTTGGAGGTTTTTTA+AGG - chr4.3:84302709-84302728 MS.gene33667:intron 25.0%
!!! GTTTTTTAAGGGAATGAAAT+TGG - chr4.3:84302721-84302740 MS.gene33667:intron 25.0%
!!! TATAACACTTTTTTAGGTCT+AGG + chr4.3:84304941-84304960 None:intergenic 25.0%
AATAACGAAGTCTTCAAACA+AGG - chr4.3:84303404-84303423 MS.gene33667:CDS 30.0%
AATGAAATTGGAGGTTAATG+TGG - chr4.3:84302733-84302752 MS.gene33667:intron 30.0%
ATAACGAAGTCTTCAAACAA+GGG - chr4.3:84303405-84303424 MS.gene33667:CDS 30.0%
ATATCAACTGATGCAATCAA+AGG + chr4.3:84303550-84303569 None:intergenic 30.0%
ATTCTACGGATCAGATAATT+TGG - chr4.3:84303156-84303175 MS.gene33667:CDS 30.0%
GAACAACTTATATTAGCAGA+TGG - chr4.3:84303730-84303749 MS.gene33667:intron 30.0%
GATTATTATGAAATGGTGGT+TGG - chr4.3:84302903-84302922 MS.gene33667:intron 30.0%
GTAATTAGATGGGAGAAATA+TGG - chr4.3:84302873-84302892 MS.gene33667:intron 30.0%
GTGAAAATATTTGCACGTTA+AGG + chr4.3:84301868-84301887 None:intergenic 30.0%
GTTTAGACCCTAAAATTCTA+CGG - chr4.3:84303142-84303161 MS.gene33667:CDS 30.0%
TGAAATGCACAAATTTCCAA+TGG + chr4.3:84303947-84303966 None:intergenic 30.0%
TGGCAAAACACAAAGATTTA+AGG - chr4.3:84304028-84304047 MS.gene33667:intron 30.0%
TGTCAGAACATAAGTCATAT+TGG - chr4.3:84303673-84303692 MS.gene33667:intron 30.0%
TGTTATAACATCGATGATAG+TGG - chr4.3:84304954-84304973 MS.gene33667:intron 30.0%
! AATTTTTGCACTTGAGGATA+AGG - chr4.3:84303787-84303806 MS.gene33667:intron 30.0%
! ATCTGATCCGTAGAATTTTA+GGG + chr4.3:84303152-84303171 None:intergenic 30.0%
! CTGCAATCAATCATTTTAGA+CGG - chr4.3:84303892-84303911 MS.gene33667:CDS 30.0%
! TATCTGATCCGTAGAATTTT+AGG + chr4.3:84303153-84303172 None:intergenic 30.0%
! TGTTTTCTTGTTACATCGTT+TGG - chr4.3:84304876-84304895 MS.gene33667:intron 30.0%
!! AGTGGATTATTATGAAATGG+TGG - chr4.3:84302899-84302918 MS.gene33667:intron 30.0%
!! ATTTTGAAAGCAACTGATAC+CGG + chr4.3:84305164-84305183 None:intergenic 30.0%
!! GATAATGAACTTGATGATGA+AGG - chr4.3:84304222-84304241 MS.gene33667:intron 30.0%
!! TCTTAGATTTTGGGAGAAAT+GGG + chr4.3:84304994-84305013 None:intergenic 30.0%
!! TGAAGTTGATTAGTTTGAAG+TGG - chr4.3:84302282-84302301 MS.gene33667:CDS 30.0%
!! TGTAAGTTGGTTACAGATAT+GGG - chr4.3:84302227-84302246 MS.gene33667:intron 30.0%
!! TTTTAAGGGAATGAAATTGG+AGG - chr4.3:84302724-84302743 MS.gene33667:intron 30.0%
!!! ATTTTTTGGTGGATTATTGG+TGG - chr4.3:84302622-84302641 MS.gene33667:intron 30.0%
!!! TAGAATTTTAGGGTCTAAAC+TGG + chr4.3:84303142-84303161 None:intergenic 30.0%
!!! TCTTGGAATTTTGATTTTCC+AGG - chr4.3:84303828-84303847 MS.gene33667:intron 30.0%
AACTGTAATTTCCAACACCT+TGG + chr4.3:84305501-84305520 None:intergenic 35.0%
AAGCAGCATTAATCATCTGA+AGG + chr4.3:84304779-84304798 None:intergenic 35.0%
ACAGGTTTGTGAATCAAATG+CGG - chr4.3:84304370-84304389 MS.gene33667:intron 35.0%
ACTGTAATTTCCAACACCTT+GGG + chr4.3:84305500-84305519 None:intergenic 35.0%
AGGTTAATGTGGAAAAAGTG+GGG - chr4.3:84302744-84302763 MS.gene33667:intron 35.0%
ATACCTCAGTTACAGATGTA+GGG - chr4.3:84305125-84305144 MS.gene33667:CDS 35.0%
ATGGAGAGATAAAGAGTTTC+AGG - chr4.3:84305342-84305361 MS.gene33667:CDS 35.0%
ATGGGAGAAATATGGTTAAG+TGG - chr4.3:84302881-84302900 MS.gene33667:intron 35.0%
ATTGCAACATCTCGAAGATT+TGG - chr4.3:84303343-84303362 MS.gene33667:CDS 35.0%
ATTGCACTAGCATTACTGAT+AGG - chr4.3:84304799-84304818 MS.gene33667:intron 35.0%
CATTAATCATCTGAAGGCTA+GGG + chr4.3:84304773-84304792 None:intergenic 35.0%
CGTTAAGGAGAATGATTTCA+AGG + chr4.3:84301853-84301872 None:intergenic 35.0%
CTAAGCTTAAGCAAATGTTC+AGG - chr4.3:84303976-84303995 MS.gene33667:intron 35.0%
CTCCTTTATCAGTTATGTTC+AGG + chr4.3:84304302-84304321 None:intergenic 35.0%
CTTTATCTCTCCATTCAAAC+AGG + chr4.3:84305336-84305355 None:intergenic 35.0%
GAGGTTAATGTGGAAAAAGT+GGG - chr4.3:84302743-84302762 MS.gene33667:intron 35.0%
GATCAAGCTCTTCAAGATAA+TGG + chr4.3:84304197-84304216 None:intergenic 35.0%
GTTTGAAGACTTCGTTATTG+AGG + chr4.3:84303403-84303422 None:intergenic 35.0%
TAAAGGAACTGGATCTTTAC+AGG - chr4.3:84304352-84304371 MS.gene33667:intron 35.0%
TACATCTGTAACTGAGGTAT+AGG + chr4.3:84305125-84305144 None:intergenic 35.0%
TATACCTCAGTTACAGATGT+AGG - chr4.3:84305124-84305143 MS.gene33667:CDS 35.0%
TATATTAGCAGATGGTTCTC+CGG - chr4.3:84303738-84303757 MS.gene33667:intron 35.0%
TCGAGATTATCAGTACTTAC+CGG + chr4.3:84302389-84302408 None:intergenic 35.0%
TCTTGTTACATCGTTTGGTT+TGG - chr4.3:84304881-84304900 MS.gene33667:intron 35.0%
TGCCTGAACATAACTGATAA+AGG - chr4.3:84304297-84304316 MS.gene33667:intron 35.0%
TGCTGTGAGATGTGTTAATT+TGG - chr4.3:84302109-84302128 MS.gene33667:CDS 35.0%
TTGCACTAGCATTACTGATA+GGG - chr4.3:84304800-84304819 MS.gene33667:intron 35.0%
TTGCTCAAAGTTAAAGGAAC+TGG - chr4.3:84304341-84304360 MS.gene33667:intron 35.0%
TTGGTTGAATAGGTGTAAGT+TGG - chr4.3:84302214-84302233 MS.gene33667:intron 35.0%
! AATCTTTGTGTTTTGCCACA+AGG + chr4.3:84304026-84304045 None:intergenic 35.0%
! ATTAGTTTGAAGTGGTGTGT+TGG - chr4.3:84302290-84302309 MS.gene33667:CDS 35.0%
! CATGTGTTGCTCAAAGTTAA+AGG - chr4.3:84304335-84304354 MS.gene33667:intron 35.0%
! TCGGAAGCGTATATATTGAT+TGG - chr4.3:84304162-84304181 MS.gene33667:intron 35.0%
! TGTGTTGCTAAATGGTAAGA+TGG + chr4.3:84305386-84305405 None:intergenic 35.0%
! TTTCTCCCAAAATCTAAGAC+AGG - chr4.3:84304995-84305014 MS.gene33667:intron 35.0%
! TTTGGATCTCTCTTATTTGC+CGG - chr4.3:84302367-84302386 MS.gene33667:CDS 35.0%
!! AAGGTTTGTTCGTTGTGTTT+TGG + chr4.3:84301834-84301853 None:intergenic 35.0%
!! CTTCTGAGAGTGTTTCAAAT+GGG + chr4.3:84301779-84301798 None:intergenic 35.0%
!! GTAAGTTGGTTACAGATATG+GGG - chr4.3:84302228-84302247 MS.gene33667:intron 35.0%
!! GTCTTAGATTTTGGGAGAAA+TGG + chr4.3:84304995-84305014 None:intergenic 35.0%
!! GTGTAAGTTGGTTACAGATA+TGG - chr4.3:84302226-84302245 MS.gene33667:intron 35.0%
!! TAACATCGATGATAGTGGTA+TGG - chr4.3:84304959-84304978 MS.gene33667:intron 35.0%
!! TCTTCTGAGAGTGTTTCAAA+TGG + chr4.3:84301780-84301799 None:intergenic 35.0%
!! TGTGTTGGTATTGCTGATTT+AGG - chr4.3:84302305-84302324 MS.gene33667:CDS 35.0%
!! TTTAGGGTGTTGATAATTGC+TGG - chr4.3:84302836-84302855 MS.gene33667:intron 35.0%
!!! AACACTTTTTTAGGTCTAGG+CGG + chr4.3:84304938-84304957 None:intergenic 35.0%
!!! TTAAGTTTTGGTTGCCGATA+AGG - chr4.3:84303458-84303477 MS.gene33667:intron 35.0%
AAGTCTCACTTCTCTCAAGA+TGG - chr4.3:84304119-84304138 MS.gene33667:intron 40.0%
AATGCTCGCAATCGAAACAT+CGG + chr4.3:84304085-84304104 None:intergenic 40.0%
AATGTGGAAAAAGTGGGGTA+TGG - chr4.3:84302749-84302768 MS.gene33667:intron 40.0%
ACATGTCTGATGAGAAGCTT+GGG + chr4.3:84305298-84305317 None:intergenic 40.0%
ACGCTCCAGCGATTAAATTA+AGG + chr4.3:84302019-84302038 None:intergenic 40.0%
ACGTCCCTTAATTTAATCGC+TGG - chr4.3:84302011-84302030 MS.gene33667:CDS 40.0%
AGTCGATATCTCGTTGTTCT+AGG - chr4.3:84304250-84304269 MS.gene33667:intron 40.0%
ATAATCTCGAGTTCGACCAA+TGG - chr4.3:84302399-84302418 MS.gene33667:CDS 40.0%
CAGGTTTGTGAATCAAATGC+GGG - chr4.3:84304371-84304390 MS.gene33667:intron 40.0%
CGCTCCAGCGATTAAATTAA+GGG + chr4.3:84302018-84302037 None:intergenic 40.0%
CTGCAGAAACTTGATATCTC+AGG - chr4.3:84303649-84303668 MS.gene33667:intron 40.0%
GAGAAGTGGTTGTATGTGTT+CGG + chr4.3:84301934-84301953 None:intergenic 40.0%
GATAAAGGAGTTGCCAATGT+TGG - chr4.3:84304312-84304331 MS.gene33667:intron 40.0%
GATTATGAAACTGTAGGGGT+TGG - chr4.3:84302956-84302975 MS.gene33667:CDS 40.0%
GCATTAATCATCTGAAGGCT+AGG + chr4.3:84304774-84304793 None:intergenic 40.0%
GGAGGTTAATGTGGAAAAAG+TGG - chr4.3:84302742-84302761 MS.gene33667:intron 40.0%
TACATGTCTGATGAGAAGCT+TGG + chr4.3:84305299-84305318 None:intergenic 40.0%
TCAAAGCTAACGAGCATTTC+TGG - chr4.3:84303700-84303719 MS.gene33667:intron 40.0%
TCAGAAGTTCAGTGCTATAG+AGG - chr4.3:84304567-84304586 MS.gene33667:intron 40.0%
TCCAGCGATTGAATTTATCG+CGG - chr4.3:84302051-84302070 MS.gene33667:CDS 40.0%
TGCACAGGAGTAACAGATTT+GGG - chr4.3:84304723-84304742 MS.gene33667:intron 40.0%
TTGCAGACACTTGAAATTCG+AGG - chr4.3:84304852-84304871 MS.gene33667:intron 40.0%
TTGGTGTAACGTTTGAGAAG+TGG + chr4.3:84301948-84301967 None:intergenic 40.0%
TTGGTTACAGATATGGGGAT+TGG - chr4.3:84302233-84302252 MS.gene33667:intron 40.0%
! AATGGCTTTTAATCCGTCAG+AGG + chr4.3:84303929-84303948 None:intergenic 40.0%
! ACAAGGGTGCAAGACATTAA+AGG - chr4.3:84303421-84303440 MS.gene33667:intron 40.0%
! ATAATTGCTGGTGATGAGGT+GGG - chr4.3:84302848-84302867 MS.gene33667:intron 40.0%
! GAGATCGAGATTTTCTACGT+TGG + chr4.3:84301967-84301986 None:intergenic 40.0%
! TCGAAACATCGGTTATCTTG+CGG + chr4.3:84304074-84304093 None:intergenic 40.0%
!! AACAACGAGATATCGACTCA+AGG + chr4.3:84304248-84304267 None:intergenic 40.0%
!! GTTGATAATTGCTGGTGATG+AGG - chr4.3:84302844-84302863 MS.gene33667:intron 40.0%
!! TCTGACGGATTAAAAGCCAT+TGG - chr4.3:84303928-84303947 MS.gene33667:CDS 40.0%
!!! ACTTTTTTGGCGGTCCTTAT+CGG + chr4.3:84303475-84303494 None:intergenic 40.0%
!!! TTTTGTCTTTTCAGGTGCAC+AGG - chr4.3:84304708-84304727 MS.gene33667:intron 40.0%
AAGCCCTACATCTGTAACTG+AGG + chr4.3:84305131-84305150 None:intergenic 45.0%
ACCGCGATAAATTCAATCGC+TGG + chr4.3:84302055-84302074 None:intergenic 45.0%
CTGTGAATTTGGAGAGGCTT+TGG - chr4.3:84302195-84302214 MS.gene33667:intron 45.0%
CTTGAAGGATGCTTTGGCAT+TGG - chr4.3:84303368-84303387 MS.gene33667:CDS 45.0%
GAGGTGGGGAGTAATTAGAT+GGG - chr4.3:84302863-84302882 MS.gene33667:intron 45.0%
GATCGACGAGAGACATTTCT+CGG + chr4.3:84303318-84303337 None:intergenic 45.0%
GATTGAATTTATCGCGGTCG+AGG - chr4.3:84302057-84302076 MS.gene33667:CDS 45.0%
GGAATCTCAGCAATTGCTTC+TGG - chr4.3:84304744-84304763 MS.gene33667:intron 45.0%
GGCTGCAATATTACCTCTGA+CGG - chr4.3:84303913-84303932 MS.gene33667:CDS 45.0%
GTCAATATCGTGTCAGACAC+CGG - chr4.3:84304532-84304551 MS.gene33667:intron 45.0%
GTGCACAGGAGTAACAGATT+TGG - chr4.3:84304722-84304741 MS.gene33667:intron 45.0%
TGAGCAACACATGCCAACAT+TGG + chr4.3:84304328-84304347 None:intergenic 45.0%
TGAGGTGGGGAGTAATTAGA+TGG - chr4.3:84302862-84302881 MS.gene33667:intron 45.0%
TTAAGGGATGCTGCTATGGT+TGG - chr4.3:84302161-84302180 MS.gene33667:intron 45.0%
TTGGTCCTTGAAGGATGCTT+TGG - chr4.3:84303362-84303381 MS.gene33667:CDS 45.0%
! AGCACTGAACTTCTGATCGA+AGG + chr4.3:84304563-84304582 None:intergenic 45.0%
! ATCAGACATGTAGAAGCACG+TGG - chr4.3:84305307-84305326 MS.gene33667:CDS 45.0%
! CAATGCCAAAGCATCCTTCA+AGG + chr4.3:84303370-84303389 None:intergenic 45.0%
! CTCGAAGATTTGGTCCTTGA+AGG - chr4.3:84303353-84303372 MS.gene33667:CDS 45.0%
! GATAATTGCTGGTGATGAGG+TGG - chr4.3:84302847-84302866 MS.gene33667:intron 45.0%
! GTCGTGTACGCTAGTTTCAT+CGG - chr4.3:84304143-84304162 MS.gene33667:intron 45.0%
! GTTCTTTTTGTCCCTTCGAC+AGG - chr4.3:84305464-84305483 MS.gene33667:CDS 45.0%
! TAATTGCTGGTGATGAGGTG+GGG - chr4.3:84302849-84302868 MS.gene33667:intron 45.0%
! TGGAGAGGCTTTGGTTGAAT+AGG - chr4.3:84302204-84302223 MS.gene33667:intron 45.0%
!! GGGATTGGTTGTATTGCAGT+TGG - chr4.3:84302248-84302267 MS.gene33667:intron 45.0%
!!! ATCGCGGTCGAGGTTTTTTA+CGG - chr4.3:84302067-84302086 MS.gene33667:CDS 45.0%
!!! GTTCGTTGTGTTTTGGTTCG+AGG + chr4.3:84301827-84301846 None:intergenic 45.0%
!!! TCGAGGTTTTTTACGGGGAA+TGG - chr4.3:84302074-84302093 MS.gene33667:CDS 45.0%
!!! TTTTGGGAGAAATGGGCTAG+TGG + chr4.3:84304987-84305006 None:intergenic 45.0%
!!! CAAAAAATAATTTTTTTTTA+AGG + chr4.3:84302610-84302629 None:intergenic 5.0%
!!! CTTAAAAAAAAATTATTTTT+TGG - chr4.3:84302608-84302627 MS.gene33667:intron 5.0%
CAACAAGAGCAAGAGTCACC+TGG + chr4.3:84303849-84303868 None:intergenic 50.0%
CACGTGGATGCCTGTTTGAA+TGG - chr4.3:84305323-84305342 MS.gene33667:CDS 50.0%
CTGAATTGGACCCAAGGTGT+TGG - chr4.3:84305487-84305506 MS.gene33667:CDS 50.0%
GGAGTTAAGGGATGCTGCTA+TGG - chr4.3:84302157-84302176 MS.gene33667:intron 50.0%
GTCCAATTCAGCCTGTCGAA+GGG + chr4.3:84305478-84305497 None:intergenic 50.0%
TCAGGCAGATTCCCAGTTTC+AGG + chr4.3:84304284-84304303 None:intergenic 50.0%
TGACGCTCTCTCTTTCCTTG+TGG - chr4.3:84304008-84304027 MS.gene33667:intron 50.0%
TGTATGTGTTCGGCGCGTAA+GGG + chr4.3:84301924-84301943 None:intergenic 50.0%
TGTTGCACGCGCTGTGAATT+TGG - chr4.3:84302184-84302203 MS.gene33667:intron 50.0%
TTGTATGTGTTCGGCGCGTA+AGG + chr4.3:84301925-84301944 None:intergenic 50.0%
!! CTTCTGATCGAAGGAGCATC+CGG + chr4.3:84304554-84304573 None:intergenic 50.0%
!! GCTTGATTTGTCGAACGCGA+CGG - chr4.3:84302136-84302155 MS.gene33667:CDS 50.0%
!!! GTTTTGGTTCGAGGAAGTCG+AGG + chr4.3:84301818-84301837 None:intergenic 50.0%
!!! TCGCGGTCGAGGTTTTTTAC+GGG - chr4.3:84302068-84302087 MS.gene33667:CDS 50.0%
AGGCTCTCCAGCCTGAAACT+GGG - chr4.3:84304270-84304289 MS.gene33667:intron 55.0%
CACGCGCTGTGAATTTGGAG+AGG - chr4.3:84302189-84302208 MS.gene33667:intron 55.0%
GACAGGCTGAATTGGACCCA+AGG - chr4.3:84305481-84305500 MS.gene33667:CDS 55.0%
GCAGATTCCCAGTTTCAGGC+TGG + chr4.3:84304280-84304299 None:intergenic 55.0%
GGTCCAATTCAGCCTGTCGA+AGG + chr4.3:84305479-84305498 None:intergenic 55.0%
GTCATTCACGCGCGGACATA+AGG + chr4.3:84301991-84302010 None:intergenic 55.0%
GTCCCTTCGACAGGCTGAAT+TGG - chr4.3:84305473-84305492 MS.gene33667:CDS 55.0%
GTCGAACGCGACGGAGTTAA+GGG - chr4.3:84302145-84302164 MS.gene33667:CDS 55.0%
TAGCCCTCCGCATGCCAATA+AGG + chr4.3:84305236-84305255 None:intergenic 55.0%
TAGGCTCTCCAGCCTGAAAC+TGG - chr4.3:84304269-84304288 MS.gene33667:intron 55.0%
TGTCGAACGCGACGGAGTTA+AGG - chr4.3:84302144-84302163 MS.gene33667:CDS 55.0%
TTCACCCTGCTTCACCTGCA+GGG - chr4.3:84305184-84305203 MS.gene33667:CDS 55.0%
TTTCACCCTGCTTCACCTGC+AGG - chr4.3:84305183-84305202 MS.gene33667:CDS 55.0%
! AGGACTTGCAGCGGCCTTAT+TGG - chr4.3:84305219-84305238 MS.gene33667:CDS 55.0%
!!! CGCGGTCGAGGTTTTTTACG+GGG - chr4.3:84302069-84302088 MS.gene33667:CDS 55.0%
AAGGCCGCTGCAAGTCCTTG+TGG + chr4.3:84305217-84305236 None:intergenic 60.0%
GGCTCCACAAGGACTTGCAG+CGG - chr4.3:84305210-84305229 MS.gene33667:CDS 60.0%
GTAAGGGACACAGAGCGCGT+CGG + chr4.3:84301908-84301927 None:intergenic 60.0%
! AGGGACGTGTCATTCACGCG+CGG + chr4.3:84301999-84302018 None:intergenic 60.0%
! GTAGGGCTTCTGTCGCTTGC+CGG - chr4.3:84305142-84305161 MS.gene33667:CDS 60.0%
!! CGGCCTTATTGGCATGCGGA+GGG - chr4.3:84305230-84305249 MS.gene33667:CDS 60.0%
!! GCAGCGGCCTTATTGGCATG+CGG - chr4.3:84305226-84305245 MS.gene33667:CDS 60.0%
CACGCCCTGCAGGTGAAGCA+GGG + chr4.3:84305191-84305210 None:intergenic 65.0%
!! GCGGCCTTATTGGCATGCGG+AGG - chr4.3:84305229-84305248 MS.gene33667:CDS 65.0%
CCACGCCCTGCAGGTGAAGC+AGG + chr4.3:84305192-84305211 None:intergenic 70.0%
CCTGCTTCACCTGCAGGGCG+TGG - chr4.3:84305189-84305208 MS.gene33667:CDS 70.0%
CTGCAGGGCGTGGCTCCACA+AGG - chr4.3:84305199-84305218 MS.gene33667:CDS 70.0%
CTTGTGGAGCCACGCCCTGC+AGG + chr4.3:84305201-84305220 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 84301747 84305539 84301747 ID=MS.gene33667
chr4.3 mRNA 84301747 84305539 84301747 ID=MS.gene33667.t1;Parent=MS.gene33667
chr4.3 exon 84305059 84305539 84305059 ID=MS.gene33667.t1.exon1;Parent=MS.gene33667.t1
chr4.3 CDS 84305059 84305539 84305059 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
chr4.3 exon 84304898 84304959 84304898 ID=MS.gene33667.t1.exon2;Parent=MS.gene33667.t1
chr4.3 CDS 84304898 84304959 84304898 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
chr4.3 exon 84303844 84303975 84303844 ID=MS.gene33667.t1.exon3;Parent=MS.gene33667.t1
chr4.3 CDS 84303844 84303975 84303844 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
chr4.3 exon 84303527 84303631 84303527 ID=MS.gene33667.t1.exon4;Parent=MS.gene33667.t1
chr4.3 CDS 84303527 84303631 84303527 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
chr4.3 exon 84302913 84303436 84302913 ID=MS.gene33667.t1.exon5;Parent=MS.gene33667.t1
chr4.3 CDS 84302913 84303436 84302913 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
chr4.3 exon 84302270 84302564 84302270 ID=MS.gene33667.t1.exon6;Parent=MS.gene33667.t1
chr4.3 CDS 84302270 84302564 84302270 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
chr4.3 exon 84301923 84302174 84301923 ID=MS.gene33667.t1.exon7;Parent=MS.gene33667.t1
chr4.3 CDS 84301923 84302174 84301923 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
chr4.3 exon 84301747 84301800 84301747 ID=MS.gene33667.t1.exon8;Parent=MS.gene33667.t1
chr4.3 CDS 84301747 84301800 84301747 ID=cds.MS.gene33667.t1;Parent=MS.gene33667.t1
Gene Sequence

>MS.gene33667

ATGAAGAAACAGAAGATTTCACAACAAAACCCATTTGAAACACTCTCAGAAGAACTCATATTCACAATCCTCGACTTCCTCGAACCAAAACACAACGAACAAACCTTGAAATCATTCTCCTTAACGTGCAAATATTTTCACGCGCTTGAATCAAAACACCGACGCGCTCTGTGTCCCTTACGCGCCGAACACATACAACCACTTCTCAAACGTTACACCAACGTAGAAAATCTCGATCTCACCTTATGTCCGCGCGTGAATGACACGTCCCTTAATTTAATCGCTGGAGCGTATAATTCTACGCTCCAGCGATTGAATTTATCGCGGTCGAGGTTTTTTACGGGGAATGGTGTGTTGAATGTTGCTGTGAGATGTGTTAATTTGGTTGAGCTTGATTTGTCGAACGCGACGGAGTTAAGGGATGCTGCTATGGTTGGTGTTGCACGCGCTGTGAATTTGGAGAGGCTTTGGTTGAATAGGTGTAAGTTGGTTACAGATATGGGGATTGGTTGTATTGCAGTTGGATGTAAGAAGTTGAAGTTGATTAGTTTGAAGTGGTGTGTTGGTATTGCTGATTTAGGTGTTGATTTACTTGCCATCAAATGTAAAGAACTTTGTACTTTGGATCTCTCTTATTTGCCGGTAAGTACTGATAATCTCGAGTTCGACCAATGGATAAATAAAGTTGATAATCTGGAGATAGACCGAAAGATAAATAAAGTCTGACTAATGATTGTTTACATCGGTGTTTGAACTCAACTTTAATCGAACAATTTGTAGTTATAAAACTCATTAATCATTTGAGCTCAATTTTTTTTTGTTTAATCAACATGATAATTTTGTTATATTTATTTTTTTGTCCTTAAAAAAAAATTATTTTTTGGTGGATTATTGGTGGATAGAGATTTATTGTTAATAGTTATTTATAGTGATATGTAATAAGTCATTGTGGTTTTAATTTTCTAAATTTGGAGGTTTTTTAAGGGAATGAAATTGGAGGTTAATGTGGAAAAAGTGGGGTATGGTATTTAATGTTTTTGTAGGTTTTATTTCCAAATTGAGAAATTTGTTTCCTTTTTTGTTTTTTATTTTAGGGTGTTGATAATTGCTGGTGATGAGGTGGGGAGTAATTAGATGGGAGAAATATGGTTAAGTGGATTATTATGAAATGGTGGTTGGCTAATTTGTTTTTAGTGAAACACTAATTTAGATTATGAAACTGTAGGGGTTGGTTAATTTGATCCATAAATTAGTAAAAATCTAAAATGATTTGTGAAATTGAAAAAATCATTTTTCTTTACAAAATTGGAAATTTTTGTTCTATTATTTTTTTTTGAAAATTACTGTTCAAATTGATCAACACAAAATGAATAATTTGTTTTAAATGAATCACCAGTTTAGACCCTAAAATTCTACGGATCAGATAATTTGGTTGATTATGTTCAGTAGATTTGGTTATAAAGTTCAGTAGTTTTGGTGCTTGATTATGTTCTTTGCTTTAATTAATTGTTTTGAAATGACATGGTTGATATAACAAGTGTTATTGTGTTCTTGTGATGTGCAGATCACCGAGAAATGTCTCTCGTCGATCTTCAAATTGCAACATCTCGAAGATTTGGTCCTTGAAGGATGCTTTGGCATTGGTGATGACAGCCTCAATAACGAAGTCTTCAAACAAGGGTGCAAGACATTAAAGGTAATTGTGAAAATATTAAGTTTTGGTTGCCGATAAGGACCGCCAAAAAAGTTAAATTTGTTGTACTTTAAAAAATTCAATGCATGTTTATGTATAATTCGCAACCTTTGATTGCATCAGTTGATATAATTTTCATATTTCTTGCCTTCACATTTGCACAATTTTTTTCTTTATCTAACTTTTTTATTTAACAAATTCACTTTCTTCTGCAGAAACTTGATATCTCAGGTTGTCAGAACATAAGTCATATTGGTCTGTCAAAGCTAACGAGCATTTCTGGAAGTGTCGAACAACTTATATTAGCAGATGGTTCTCCGGTAATTCTTCAGTCTTTATAATAATTAAATTTTTGCACTTGAGGATAAGGAATTCAATTATAACTATTTCTTGGAATTTTGATTTTCCAGGTGACTCTTGCTCTTGTTGATAGTTTAAATAAACTTTCAATGCTGCAATCAATCATTTTAGACGGCTGCAATATTACCTCTGACGGATTAAAAGCCATTGGAAATTTGTGCATTTCACTCAGAGAACTAAGCTTAAGCAAATGTTCAGGAGTGACAGATGACGCTCTCTCTTTCCTTGTGGCAAAACACAAAGATTTAAGGAAGCTCGACATCACATGCTGCCGCAAGATAACCGATGTTTCGATTGCGAGCATTGCAAATGCATGCAGAAGTCTCACTTCTCTCAAGATGGAGTCGTGTACGCTAGTTTCATCGGAAGCGTATATATTGATTGGACAGAAATGCCATTATCTTGAAGAGCTTGATCTCACAGATAATGAACTTGATGATGAAGGCCTTGAGTCGATATCTCGTTGTTCTAGGCTCTCCAGCCTGAAACTGGGAATCTGCCTGAACATAACTGATAAAGGAGTTGCCAATGTTGGCATGTGTTGCTCAAAGTTAAAGGAACTGGATCTTTACAGGTTTGTGAATCAAATGCGGGCCTTTGCTTTTCTTGTCAATTTTAAGGTGTTTTTTTGGATAAACAGCTTACTTAAGTGTTTATCATGTTAGTGCTTATGCTAAACTATTTTTATAACAAAAGATAAAATAAGGTCAAACTGTTTTTATAGTCACATTTGTCAATATCGTGTCAGACACCGGATGCTCCTTCGATCAGAAGTTCAGTGCTATAGAGGATGTACCTTATCTAAAGTAGATTTTACAAAATATGGCTTTGACATAAAAGTTCTATGATTGTACCTTATTAGATGTTGACATAAATATTATTGTCCAATGTCAACTAATTAAGATTATTTTTGTCTTTTCAGGTGCACAGGAGTAACAGATTTGGGAATCTCAGCAATTGCTTCTGGTTGCCCTAGCCTTCAGATGATTAATGCTGCTTATTGCACTAGCATTACTGATAGGGCATTATTCTGCTTGTCAAAATGTGTAAATTTGCAGACACTTGAAATTCGAGGATGTTTTCTTGTTACATCGTTTGGTTTGGCATGTATTGCAATGAATTGCAAACAACTAAGCCGCCTAGACCTAAAAAAGTGTTATAACATCGATGATAGTGGTATGGTTCCACTAGCCCATTTCTCCCAAAATCTAAGACAGGTATTTTCGTCATTTCTCAATTTTCTGCAATACATTAAAGATGCAAGATAGCTATGCTAGAGATTCTGATGTTATATCTTATTCGTCATTTGCAGATAAACTTGTCCTATACCTCAGTTACAGATGTAGGGCTTCTGTCGCTTGCCGGTATCAGTTGCTTTCAAAATTTCACCCTGCTTCACCTGCAGGGCGTGGCTCCACAAGGACTTGCAGCGGCCTTATTGGCATGCGGAGGGCTAACAAAAGCAAAGCTTCATGTTAAACTAAGATCTTTATTACCCAAGCTTCTCATCAGACATGTAGAAGCACGTGGATGCCTGTTTGAATGGAGAGATAAAGAGTTTCAGGTATATTCGTTTTTCGTTTCCATCTTACCATTTAGCAACACATTTTATGAAAATGCTAGAGTTCTTCCTCATTTTTTTCGCATTTTATTTGGTCATTGAAGTTCTTTTTGTCCCTTCGACAGGCTGAATTGGACCCAAGGTGTTGGAAATTACAGTTAGAAGATCTGATGCATTAG
Protein sequence

>MS.gene33667.t1

MKKQKISQQNPFETLSEELIFTILDFLEPKHNEQTLKSFSLTCKYFHALESKHRRALCPLRAEHIQPLLKRYTNVENLDLTLCPRVNDTSLNLIAGAYNSTLQRLNLSRSRFFTGNGVLNVAVRCVNLVELDLSNATELRDAAMVGVARAVNLERLWLNRCVDLLAIKCKELCTLDLSYLPITEKCLSSIFKLQHLEDLVLEGCFGIGDDSLNNEVFKQGCKTLKKLDISGCQNISHIGLSKLTSISGSVEQLILADGSPVTLALVDSLNKLSMLQSIILDGCNITSDGLKAIGNLCISLRELSLSKCSGVTDDALSFLVAKHKDLRKLDITCCRKITDVSIASIANACRSLTSLKMESCTLVSSEAYILIGQKCHYLEELDLTDNELDDEGLESISRCSRLSSLKLGICLNITDKGVANVGMCCSKLKELDLYRCTGVTDLGISAIASGCPSLQMINAAYCTSITDRALFCLSKCVNLQTLEIRGCFLVTSFGLACIAMNCKQLSRLDLKKCYNIDDSGMVPLAHFSQNLRQINLSYTSVTDVGLLSLAGISCFQNFTLLHLQGVAPQGLAAALLACGGLTKAKLHVKLRSLLPKLLIRHVEARGCLFEWRDKEFQAELDPRCWKLQLEDLMH