Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79719.t1 | XP_013444708.1 | 98 | 395 | 8 | 0 | 1 | 395 | 1 | 395 | 1.10E-96 | 363.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79719.t1 | Q9SRR1 | 50.2 | 295 | 141 | 3 | 8 | 297 | 1 | 294 | 1.5e-41 | 171.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79719.t1 | A0A072TPF2 | 98.0 | 395 | 8 | 0 | 1 | 395 | 1 | 395 | 8.3e-97 | 363.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene79719 | MS.gene005659 | PPI |
| MS.gene79719 | MS.gene84072 | PPI |
| MS.gene79719 | MS.gene068166 | PPI |
| MS.gene79719 | MS.gene07153 | PPI |
| MS.gene79719 | MS.gene040704 | PPI |
| MS.gene79719 | MS.gene21545 | PPI |
| MS.gene79719 | MS.gene84069 | PPI |
| MS.gene79719 | MS.gene04676 | PPI |
| MS.gene79719 | MS.gene073670 | PPI |
| MS.gene79719 | MS.gene52721 | PPI |
| MS.gene79719 | MS.gene055944 | PPI |
| MS.gene79719 | MS.gene64806 | PPI |
| MS.gene79719 | MS.gene040706 | PPI |
| MS.gene79719 | MS.gene073674 | PPI |
| MS.gene79719 | MS.gene60611 | PPI |
| MS.gene79719 | MS.gene02998 | PPI |
| MS.gene79719 | MS.gene068164 | PPI |
| MS.gene79719 | MS.gene59505 | PPI |
| MS.gene79719 | MS.gene67485 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79719.t1 | MTR_8g028140 | 97.215 | 395 | 11 | 0 | 1 | 395 | 1 | 395 | 0.0 | 774 |
| MS.gene79719.t1 | MTR_8g028140 | 96.753 | 308 | 10 | 0 | 88 | 395 | 8 | 315 | 0.0 | 597 |
| MS.gene79719.t1 | MTR_2g021030 | 28.198 | 383 | 236 | 10 | 22 | 368 | 11 | 390 | 7.30e-31 | 123 |
| MS.gene79719.t1 | MTR_2g021030 | 28.115 | 313 | 165 | 9 | 52 | 334 | 152 | 434 | 3.55e-25 | 107 |
| MS.gene79719.t1 | MTR_2g021030 | 28.198 | 383 | 236 | 10 | 22 | 368 | 11 | 390 | 2.30e-30 | 123 |
| MS.gene79719.t1 | MTR_2g021030 | 26.558 | 369 | 192 | 9 | 52 | 368 | 152 | 493 | 2.59e-23 | 102 |
| MS.gene79719.t1 | MTR_2g021030 | 29.588 | 267 | 169 | 9 | 75 | 333 | 333 | 588 | 8.02e-20 | 91.7 |
| MS.gene79719.t1 | MTR_2g021030 | 28.205 | 273 | 190 | 4 | 97 | 364 | 296 | 567 | 1.64e-18 | 87.8 |
| MS.gene79719.t1 | MTR_2g021030 | 26.515 | 264 | 186 | 5 | 95 | 352 | 320 | 581 | 3.33e-16 | 80.9 |
| MS.gene79719.t1 | MTR_1g114610 | 28.693 | 352 | 211 | 8 | 22 | 368 | 20 | 336 | 5.41e-30 | 120 |
| MS.gene79719.t1 | MTR_1g012520 | 27.704 | 379 | 243 | 7 | 7 | 368 | 53 | 417 | 1.03e-26 | 112 |
| MS.gene79719.t1 | MTR_1g012520 | 27.000 | 300 | 188 | 7 | 82 | 374 | 360 | 635 | 2.63e-17 | 84.3 |
| MS.gene79719.t1 | MTR_1g012520 | 22.849 | 372 | 217 | 9 | 66 | 374 | 193 | 557 | 4.18e-14 | 74.3 |
| MS.gene79719.t1 | MTR_7g112570 | 25.467 | 428 | 226 | 14 | 21 | 385 | 48 | 445 | 8.78e-23 | 100 |
| MS.gene79719.t1 | MTR_7g112570 | 32.934 | 167 | 83 | 3 | 97 | 235 | 442 | 607 | 3.05e-19 | 90.1 |
| MS.gene79719.t1 | MTR_7g112570 | 26.502 | 283 | 195 | 9 | 95 | 370 | 363 | 639 | 9.96e-17 | 82.4 |
| MS.gene79719.t1 | MTR_7g112570 | 25.467 | 428 | 226 | 14 | 21 | 385 | 48 | 445 | 1.26e-22 | 100 |
| MS.gene79719.t1 | MTR_7g112570 | 34.459 | 148 | 71 | 2 | 97 | 219 | 442 | 588 | 2.43e-18 | 87.4 |
| MS.gene79719.t1 | MTR_7g112570 | 27.273 | 253 | 156 | 6 | 95 | 345 | 363 | 589 | 2.04e-15 | 78.6 |
| MS.gene79719.t1 | MTR_7g112570 | 23.273 | 275 | 182 | 6 | 91 | 363 | 334 | 581 | 8.85e-14 | 73.2 |
| MS.gene79719.t1 | MTR_7g112570 | 25.467 | 428 | 226 | 14 | 21 | 385 | 48 | 445 | 1.37e-22 | 100 |
| MS.gene79719.t1 | MTR_7g112570 | 34.459 | 148 | 71 | 2 | 97 | 219 | 442 | 588 | 2.43e-18 | 87.4 |
| MS.gene79719.t1 | MTR_7g112570 | 27.273 | 253 | 156 | 6 | 95 | 345 | 363 | 589 | 2.01e-15 | 78.6 |
| MS.gene79719.t1 | MTR_7g112570 | 23.273 | 275 | 182 | 6 | 91 | 363 | 334 | 581 | 8.00e-14 | 73.6 |
| MS.gene79719.t1 | MTR_3g107280 | 27.005 | 374 | 243 | 8 | 17 | 376 | 66 | 423 | 7.08e-21 | 95.1 |
| MS.gene79719.t1 | MTR_3g107280 | 21.858 | 366 | 216 | 9 | 66 | 368 | 191 | 549 | 1.89e-12 | 69.3 |
| MS.gene79719.t1 | MTR_4g114640 | 26.404 | 356 | 241 | 8 | 18 | 364 | 60 | 403 | 1.05e-19 | 91.7 |
| MS.gene79719.t1 | MTR_4g013255 | 27.124 | 306 | 166 | 10 | 93 | 368 | 227 | 505 | 1.11e-18 | 88.6 |
| MS.gene79719.t1 | MTR_4g013255 | 24.723 | 271 | 195 | 7 | 97 | 361 | 335 | 602 | 7.59e-15 | 76.6 |
| MS.gene79719.t1 | MTR_4g013255 | 29.918 | 244 | 139 | 11 | 92 | 331 | 408 | 623 | 1.86e-14 | 75.5 |
| MS.gene79719.t1 | MTR_1g054625 | 25.571 | 219 | 154 | 3 | 91 | 301 | 75 | 292 | 4.77e-17 | 82.0 |
| MS.gene79719.t1 | MTR_1g018790 | 26.211 | 351 | 179 | 14 | 19 | 359 | 61 | 341 | 1.40e-15 | 79.0 |
| MS.gene79719.t1 | MTR_1g054625 | 26.230 | 244 | 148 | 5 | 122 | 360 | 13 | 229 | 2.01e-15 | 75.9 |
| MS.gene79719.t1 | MTR_1g054625 | 28.947 | 152 | 98 | 4 | 92 | 241 | 61 | 204 | 5.07e-11 | 63.2 |
| MS.gene79719.t1 | MTR_3g088895 | 35.922 | 103 | 65 | 1 | 95 | 196 | 180 | 282 | 7.26e-12 | 66.6 |
| MS.gene79719.t1 | MTR_1g029500 | 30.000 | 160 | 92 | 4 | 73 | 219 | 152 | 304 | 1.28e-11 | 65.9 |
| MS.gene79719.t1 | MTR_4g114650 | 24.079 | 353 | 224 | 10 | 18 | 364 | 55 | 369 | 8.62e-11 | 64.3 |
| MS.gene79719.t1 | MTR_4g114650 | 24.079 | 353 | 224 | 10 | 18 | 364 | 59 | 373 | 8.96e-11 | 63.9 |
| MS.gene79719.t1 | MTR_4g114650 | 24.079 | 353 | 224 | 10 | 18 | 364 | 110 | 424 | 9.09e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79719.t1 | AT3G07550 | 59.278 | 388 | 152 | 3 | 8 | 390 | 1 | 387 | 1.90e-152 | 436 |
| MS.gene79719.t1 | AT3G07550 | 59.278 | 388 | 152 | 3 | 8 | 390 | 1 | 387 | 1.90e-152 | 436 |
| MS.gene79719.t1 | AT3G07550 | 54.897 | 388 | 142 | 4 | 8 | 390 | 1 | 360 | 2.55e-133 | 387 |
| MS.gene79719.t1 | AT5G23340 | 28.649 | 370 | 237 | 6 | 22 | 366 | 10 | 377 | 1.04e-34 | 132 |
| MS.gene79719.t1 | AT4G15475 | 27.578 | 417 | 221 | 13 | 22 | 368 | 11 | 416 | 2.05e-28 | 117 |
| MS.gene79719.t1 | AT4G15475 | 28.364 | 275 | 187 | 5 | 97 | 364 | 296 | 567 | 2.09e-22 | 99.8 |
| MS.gene79719.t1 | AT4G15475 | 27.099 | 262 | 166 | 5 | 108 | 368 | 256 | 493 | 2.11e-19 | 90.5 |
| MS.gene79719.t1 | AT4G15475 | 28.058 | 278 | 169 | 7 | 95 | 368 | 218 | 468 | 3.63e-18 | 87.0 |
| MS.gene79719.t1 | AT4G15475 | 32.090 | 134 | 90 | 1 | 95 | 228 | 450 | 582 | 2.92e-13 | 71.6 |
| MS.gene79719.t1 | AT2G25490 | 26.301 | 365 | 235 | 6 | 17 | 368 | 62 | 405 | 2.66e-22 | 99.4 |
| MS.gene79719.t1 | AT2G25490 | 27.869 | 244 | 144 | 9 | 108 | 345 | 374 | 591 | 5.70e-12 | 67.8 |
| MS.gene79719.t1 | AT2G25490 | 24.923 | 325 | 172 | 9 | 90 | 361 | 250 | 555 | 5.95e-11 | 64.7 |
| MS.gene79719.t1 | AT5G25350 | 25.895 | 363 | 244 | 7 | 17 | 371 | 53 | 398 | 1.15e-21 | 97.4 |
| MS.gene79719.t1 | AT3G58530 | 26.337 | 243 | 155 | 4 | 122 | 363 | 112 | 331 | 6.41e-18 | 84.7 |
| MS.gene79719.t1 | AT3G58530 | 27.811 | 169 | 115 | 4 | 87 | 255 | 155 | 316 | 2.96e-11 | 64.7 |
| MS.gene79719.t1 | AT5G27920 | 26.786 | 336 | 202 | 10 | 66 | 369 | 99 | 422 | 4.87e-17 | 83.6 |
| MS.gene79719.t1 | AT5G27920 | 25.212 | 353 | 182 | 7 | 78 | 373 | 201 | 528 | 1.28e-16 | 82.0 |
| MS.gene79719.t1 | AT5G27920 | 26.165 | 279 | 192 | 8 | 100 | 370 | 330 | 602 | 7.73e-16 | 79.7 |
| MS.gene79719.t1 | AT5G27920 | 29.787 | 235 | 129 | 9 | 97 | 325 | 405 | 609 | 5.93e-14 | 73.9 |
| MS.gene79719.t1 | AT1G21410 | 24.204 | 314 | 164 | 9 | 90 | 376 | 85 | 351 | 5.68e-16 | 79.0 |
| MS.gene79719.t1 | AT1G21410 | 27.128 | 188 | 132 | 3 | 77 | 262 | 125 | 309 | 3.65e-13 | 70.5 |
| MS.gene79719.t1 | AT1G21410 | 24.204 | 314 | 164 | 9 | 90 | 376 | 85 | 351 | 5.68e-16 | 79.0 |
| MS.gene79719.t1 | AT1G21410 | 27.128 | 188 | 132 | 3 | 77 | 262 | 125 | 309 | 3.65e-13 | 70.5 |
| MS.gene79719.t1 | AT5G01720 | 25.329 | 304 | 215 | 7 | 90 | 385 | 273 | 572 | 9.47e-16 | 79.7 |
| MS.gene79719.t1 | AT5G01720 | 23.547 | 327 | 168 | 8 | 122 | 368 | 151 | 475 | 3.31e-13 | 71.6 |
| MS.gene79719.t1 | AT1G77000 | 35.714 | 112 | 71 | 1 | 107 | 217 | 103 | 214 | 5.03e-13 | 68.9 |
| MS.gene79719.t1 | AT1G77000 | 35.714 | 112 | 71 | 1 | 107 | 217 | 103 | 214 | 5.03e-13 | 68.9 |
| MS.gene79719.t1 | AT1G77000 | 25.352 | 284 | 161 | 10 | 107 | 375 | 103 | 350 | 7.17e-13 | 69.7 |
| MS.gene79719.t1 | AT1G77000 | 35.043 | 117 | 72 | 2 | 81 | 196 | 158 | 271 | 7.78e-13 | 69.7 |
| MS.gene79719.t1 | AT1G77000 | 27.528 | 178 | 124 | 3 | 87 | 262 | 135 | 309 | 1.26e-12 | 68.9 |
| MS.gene79719.t1 | AT1G77000 | 25.352 | 284 | 161 | 10 | 107 | 375 | 103 | 350 | 7.17e-13 | 69.7 |
| MS.gene79719.t1 | AT1G77000 | 35.043 | 117 | 72 | 2 | 81 | 196 | 158 | 271 | 7.78e-13 | 69.7 |
| MS.gene79719.t1 | AT1G77000 | 27.528 | 178 | 124 | 3 | 87 | 262 | 135 | 309 | 1.26e-12 | 68.9 |
Find 90 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTCCGACGGTTGAAATTT+TGG | 0.184394 | 8.4:-67825455 | None:intergenic |
| ACAGTTCCGATCACCCCTTC+TGG | 0.222734 | 8.4:-67826002 | None:intergenic |
| CATGCAGTCTCTTCAAATTC+TGG | 0.257571 | 8.4:-67826265 | None:intergenic |
| TGCCTCAATCTTACTGATAA+TGG | 0.307545 | 8.4:+67826296 | MS.gene79719:CDS |
| TTGCTTAAGTTGGTCACCTT+CGG | 0.318059 | 8.4:+67826058 | MS.gene79719:CDS |
| GGGCTATCCCTAGTTGCTTC+TGG | 0.330852 | 8.4:+67825708 | MS.gene79719:CDS |
| TGCTTAAGTTGGTCACCTTC+GGG | 0.345625 | 8.4:+67826059 | MS.gene79719:CDS |
| TGTCATGAGGTGAGTATATC+TGG | 0.346053 | 8.4:+67826218 | MS.gene79719:CDS |
| CCCTTCCTCTTTGTCTGCTA+AGG | 0.349759 | 8.4:+67825509 | MS.gene79719:CDS |
| TTTACACCGTCCATATTCAT+AGG | 0.354668 | 8.4:+67825541 | MS.gene79719:CDS |
| GGAGATCCCTTGCGTGGAAT+AGG | 0.355838 | 8.4:+67826080 | MS.gene79719:CDS |
| TGCACGGAGCTCGATGATTC+AGG | 0.355920 | 8.4:+67825609 | MS.gene79719:CDS |
| CGTACTGATCGTGAATCATT+TGG | 0.356173 | 8.4:+67825405 | MS.gene79719:CDS |
| GCCTTAGCAACCTATGAATA+TGG | 0.358247 | 8.4:-67825551 | None:intergenic |
| GATGGAGGTAGCACCAAGTT+TGG | 0.367641 | 8.4:-67825331 | None:intergenic |
| ACAACCATTCATGTACAAAA+TGG | 0.385976 | 8.4:-67826354 | None:intergenic |
| ATCTACACATTCGAAAGTTT+AGG | 0.413893 | 8.4:-67826124 | None:intergenic |
| TGTCTTAACATTACTGATGA+AGG | 0.429447 | 8.4:+67825765 | MS.gene79719:CDS |
| CAGAGGCGATGTTTGCATAA+AGG | 0.431376 | 8.4:+67826418 | MS.gene79719:CDS |
| AAGAGCTTTCAATCCTTTGT+CGG | 0.436470 | 8.4:-67825856 | None:intergenic |
| TCAGCCAATATCATCTGAAT+GGG | 0.438144 | 8.4:-67826472 | None:intergenic |
| CTCTACGTGTACGAGAGATT+TGG | 0.439223 | 8.4:-67825958 | None:intergenic |
| CAGTTCCGATCACCCCTTCT+GGG | 0.444359 | 8.4:-67826001 | None:intergenic |
| ATCGGAACTGTTAGCGGTGG+CGG | 0.448531 | 8.4:+67826014 | MS.gene79719:CDS |
| ATGGAAGATCTTTCCAAACT+TGG | 0.457693 | 8.4:+67825318 | MS.gene79719:CDS |
| TCTTCAGATGAGTCTTGAAA+AGG | 0.458770 | 8.4:+67825290 | None:intergenic |
| TAGATGTATCTTGCTTAAGT+TGG | 0.458772 | 8.4:+67826048 | MS.gene79719:CDS |
| ATCAGCCAATATCATCTGAA+TGG | 0.461278 | 8.4:-67826473 | None:intergenic |
| TATCATCTGAATGGGGAGTA+AGG | 0.462310 | 8.4:-67826464 | None:intergenic |
| TTCAAGTAGTGTACATCCCT+TGG | 0.473496 | 8.4:-67826177 | None:intergenic |
| TTGAATAACTCTAATGCTAA+AGG | 0.480544 | 8.4:-67826389 | None:intergenic |
| TGAACCGTAGCTTAGCAAAC+GGG | 0.481477 | 8.4:-67825637 | None:intergenic |
| CCTTCCTCTTTGTCTGCTAA+GGG | 0.491175 | 8.4:+67825510 | MS.gene79719:CDS |
| TCACACTGCGAGCGTATAAG+TGG | 0.492469 | 8.4:+67825915 | MS.gene79719:CDS |
| TTGAACCGTAGCTTAGCAAA+CGG | 0.494178 | 8.4:-67825638 | None:intergenic |
| GATGTACACTACTTGAAGAA+TGG | 0.498471 | 8.4:+67826183 | MS.gene79719:CDS |
| ATAATAGTAAGGCAGTCATC+TGG | 0.504519 | 8.4:-67825363 | None:intergenic |
| TGTTTGAAAGTCACTAACTA+CGG | 0.506597 | 8.4:+67825687 | MS.gene79719:CDS |
| TTAGAGTCTTTATCTCTATG+TGG | 0.511065 | 8.4:+67825585 | MS.gene79719:CDS |
| TTGGCTTGACTTGCCACCGA+TGG | 0.514077 | 8.4:+67825424 | MS.gene79719:CDS |
| TGCGAGCGTATAAGTGGTGT+TGG | 0.528442 | 8.4:+67825921 | MS.gene79719:CDS |
| TTCTATTATTGCAATAGCCA+AGG | 0.528487 | 8.4:+67826160 | MS.gene79719:CDS |
| GTTTGAAAGTCACTAACTAC+GGG | 0.539400 | 8.4:+67825688 | MS.gene79719:CDS |
| CTTAGCCCAGAAGGGGTGAT+CGG | 0.540124 | 8.4:+67825996 | MS.gene79719:CDS |
| ATAAGTGGTGTTGGATTCGA+AGG | 0.541644 | 8.4:+67825930 | MS.gene79719:CDS |
| AGGGACAACCAGAAGCAACT+AGG | 0.542948 | 8.4:-67825716 | None:intergenic |
| TGGGCTAAGTTTACAAGACT+CGG | 0.549702 | 8.4:-67825982 | None:intergenic |
| TCTTGTAAACTTAGCCCAGA+AGG | 0.553718 | 8.4:+67825987 | MS.gene79719:CDS |
| TCCATATTCATAGGTTGCTA+AGG | 0.554256 | 8.4:+67825550 | MS.gene79719:CDS |
| ATACGTTAATAGCTTGAAGC+TGG | 0.554829 | 8.4:-67825893 | None:intergenic |
| CCCTTAGCAGACAAAGAGGA+AGG | 0.557377 | 8.4:-67825510 | None:intergenic |
| GTCATCTGGTAAGTGCATGA+TGG | 0.563773 | 8.4:-67825349 | None:intergenic |
| ATATCTGGATGGCAAACAGT+GGG | 0.564668 | 8.4:+67826233 | MS.gene79719:CDS |
| GGGACAACCAGAAGCAACTA+GGG | 0.569174 | 8.4:-67825715 | None:intergenic |
| CTTGTAAACTTAGCCCAGAA+GGG | 0.572152 | 8.4:+67825988 | MS.gene79719:CDS |
| TGACAGCAATTAGTCTCTAT+CGG | 0.572523 | 8.4:+67825742 | MS.gene79719:CDS |
| TCTATTATTGCAATAGCCAA+GGG | 0.578035 | 8.4:+67826161 | MS.gene79719:CDS |
| GAGTCTTGAAAAGGTTGACA+TGG | 0.579264 | 8.4:+67825299 | MS.gene79719:CDS |
| AGCCATTATCAGTAAGATTG+AGG | 0.584084 | 8.4:-67826298 | None:intergenic |
| AGACTAATTGCTGTCAATGA+GGG | 0.588028 | 8.4:-67825735 | None:intergenic |
| AAGACCCTTAGCAGACAAAG+AGG | 0.588868 | 8.4:-67825514 | None:intergenic |
| GGAAAATCCTATTCCACGCA+AGG | 0.589659 | 8.4:-67826087 | None:intergenic |
| GGCTTGCGAGCGTTACGAGA+CGG | 0.593002 | 8.4:+67826317 | MS.gene79719:CDS |
| GGGGTGATCGGAACTGTTAG+CGG | 0.593998 | 8.4:+67826008 | MS.gene79719:CDS |
| CTAACCCGTTTGCTAAGCTA+CGG | 0.594245 | 8.4:+67825633 | MS.gene79719:CDS |
| GAGACTAATTGCTGTCAATG+AGG | 0.594543 | 8.4:-67825736 | None:intergenic |
| ATATCTGTGAGCAGTATGAC+AGG | 0.597576 | 8.4:-67825833 | None:intergenic |
| TTTATCTCTATGTGGTTGCA+CGG | 0.599367 | 8.4:+67825593 | MS.gene79719:CDS |
| TTTGGATATGCAGCCATCGG+TGG | 0.600131 | 8.4:-67825437 | None:intergenic |
| CAAATCTCTCGTACACGTAG+AGG | 0.609038 | 8.4:+67825959 | MS.gene79719:CDS |
| CCTTCGGGAGATCCCTTGCG+TGG | 0.615820 | 8.4:+67826074 | MS.gene79719:CDS |
| TTTCAAAGATAAGCAAGCAG+TGG | 0.631769 | 8.4:-67825802 | None:intergenic |
| CCTTAGCAGACAAAGAGGAA+GGG | 0.632936 | 8.4:-67825509 | None:intergenic |
| TGTTTGCATAAAGGAAGAAG+AGG | 0.634712 | 8.4:+67826427 | MS.gene79719:CDS |
| CCACGCAAGGGATCTCCCGA+AGG | 0.635892 | 8.4:-67826074 | None:intergenic |
| ATCTGGTAAGTGCATGATGG+AGG | 0.637213 | 8.4:-67825346 | None:intergenic |
| ATATCCAAAATTTCAACCGT+CGG | 0.640422 | 8.4:+67825451 | MS.gene79719:CDS |
| TAGCAACCTATGAATATGGA+CGG | 0.643940 | 8.4:-67825547 | None:intergenic |
| TTGTAAACTTAGCCCAGAAG+GGG | 0.645808 | 8.4:+67825989 | MS.gene79719:CDS |
| TATATCTGGATGGCAAACAG+TGG | 0.653962 | 8.4:+67826232 | MS.gene79719:CDS |
| GTGATCGGAACTGTTAGCGG+TGG | 0.655476 | 8.4:+67826011 | MS.gene79719:CDS |
| GAAAATCCTATTCCACGCAA+GGG | 0.659659 | 8.4:-67826086 | None:intergenic |
| GAACTTAGCATTATGTCATG+AGG | 0.663036 | 8.4:+67826205 | MS.gene79719:CDS |
| ACTCAAATTGCAGAGTCCGA+CGG | 0.672603 | 8.4:-67825467 | None:intergenic |
| TGCTCACAGATATCCGACAA+AGG | 0.684591 | 8.4:+67825843 | MS.gene79719:CDS |
| CAGCCAATATCATCTGAATG+GGG | 0.689748 | 8.4:-67826471 | None:intergenic |
| ATGAGGTGAGTATATCTGGA+TGG | 0.692395 | 8.4:+67826222 | MS.gene79719:CDS |
| TAAGTGGTGTTGGATTCGAA+GGG | 0.692526 | 8.4:+67825931 | MS.gene79719:CDS |
| TATCTGGATGGCAAACAGTG+GGG | 0.720797 | 8.4:+67826234 | MS.gene79719:CDS |
| GAGTTATTCAAGTCTCACAG+AGG | 0.740335 | 8.4:+67826401 | MS.gene79719:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTACAAAAACTTAATC+TGG | + | chr8.4:67825659-67825678 | MS.gene79719:CDS | 15.0% |
| !! | AACCATGAAAAATAATAGTA+AGG | - | chr8.4:67825377-67825396 | None:intergenic | 20.0% |
| ! | TTGAATAACTCTAATGCTAA+AGG | - | chr8.4:67826392-67826411 | None:intergenic | 25.0% |
| !!! | TGCCTTACTATTATTTTTCA+TGG | + | chr8.4:67825372-67825391 | MS.gene79719:CDS | 25.0% |
| ACAACCATTCATGTACAAAA+TGG | - | chr8.4:67826357-67826376 | None:intergenic | 30.0% | |
| ATATCCAAAATTTCAACCGT+CGG | + | chr8.4:67825451-67825470 | MS.gene79719:CDS | 30.0% | |
| ATCTACACATTCGAAAGTTT+AGG | - | chr8.4:67826127-67826146 | None:intergenic | 30.0% | |
| TAGATGTATCTTGCTTAAGT+TGG | + | chr8.4:67826048-67826067 | MS.gene79719:CDS | 30.0% | |
| TCTATTATTGCAATAGCCAA+GGG | + | chr8.4:67826161-67826180 | MS.gene79719:CDS | 30.0% | |
| TGTCTTAACATTACTGATGA+AGG | + | chr8.4:67825765-67825784 | MS.gene79719:CDS | 30.0% | |
| TGTTTGAAAGTCACTAACTA+CGG | + | chr8.4:67825687-67825706 | MS.gene79719:CDS | 30.0% | |
| TTAGAGTCTTTATCTCTATG+TGG | + | chr8.4:67825585-67825604 | MS.gene79719:CDS | 30.0% | |
| TTCTATTATTGCAATAGCCA+AGG | + | chr8.4:67826160-67826179 | MS.gene79719:CDS | 30.0% | |
| !! | TTCGAAAGTTTAGGATTTTG+AGG | - | chr8.4:67826118-67826137 | None:intergenic | 30.0% |
| AAGAGCTTTCAATCCTTTGT+CGG | - | chr8.4:67825859-67825878 | None:intergenic | 35.0% | |
| AGACTAATTGCTGTCAATGA+GGG | - | chr8.4:67825738-67825757 | None:intergenic | 35.0% | |
| AGCCATTATCAGTAAGATTG+AGG | - | chr8.4:67826301-67826320 | None:intergenic | 35.0% | |
| ATAATAGTAAGGCAGTCATC+TGG | - | chr8.4:67825366-67825385 | None:intergenic | 35.0% | |
| ATACGTTAATAGCTTGAAGC+TGG | - | chr8.4:67825896-67825915 | None:intergenic | 35.0% | |
| ATGGAAGATCTTTCCAAACT+TGG | + | chr8.4:67825318-67825337 | MS.gene79719:CDS | 35.0% | |
| GAACTTAGCATTATGTCATG+AGG | + | chr8.4:67826205-67826224 | MS.gene79719:CDS | 35.0% | |
| GATGTACACTACTTGAAGAA+TGG | + | chr8.4:67826183-67826202 | MS.gene79719:CDS | 35.0% | |
| GTTTGAAAGTCACTAACTAC+GGG | + | chr8.4:67825688-67825707 | MS.gene79719:CDS | 35.0% | |
| TAGCAACCTATGAATATGGA+CGG | - | chr8.4:67825550-67825569 | None:intergenic | 35.0% | |
| TCCATATTCATAGGTTGCTA+AGG | + | chr8.4:67825550-67825569 | MS.gene79719:CDS | 35.0% | |
| TGCCTCAATCTTACTGATAA+TGG | + | chr8.4:67826296-67826315 | MS.gene79719:CDS | 35.0% | |
| TGTTTGCATAAAGGAAGAAG+AGG | + | chr8.4:67826427-67826446 | MS.gene79719:CDS | 35.0% | |
| TTTACACCGTCCATATTCAT+AGG | + | chr8.4:67825541-67825560 | MS.gene79719:CDS | 35.0% | |
| TTTATCTCTATGTGGTTGCA+CGG | + | chr8.4:67825593-67825612 | MS.gene79719:CDS | 35.0% | |
| TTTCAAAGATAAGCAAGCAG+TGG | - | chr8.4:67825805-67825824 | None:intergenic | 35.0% | |
| ! | AATTTTGGATATGCAGCCAT+CGG | - | chr8.4:67825443-67825462 | None:intergenic | 35.0% |
| ! | CTCTCCATTTTGTACATGAA+TGG | + | chr8.4:67826350-67826369 | MS.gene79719:CDS | 35.0% |
| ! | TGACAGCAATTAGTCTCTAT+CGG | + | chr8.4:67825742-67825761 | MS.gene79719:CDS | 35.0% |
| ATATCTGGATGGCAAACAGT+GGG | + | chr8.4:67826233-67826252 | MS.gene79719:CDS | 40.0% | |
| ATATCTGTGAGCAGTATGAC+AGG | - | chr8.4:67825836-67825855 | None:intergenic | 40.0% | |
| ATGAGGTGAGTATATCTGGA+TGG | + | chr8.4:67826222-67826241 | MS.gene79719:CDS | 40.0% | |
| CATGCAGTCTCTTCAAATTC+TGG | - | chr8.4:67826268-67826287 | None:intergenic | 40.0% | |
| CTTGTAAACTTAGCCCAGAA+GGG | + | chr8.4:67825988-67826007 | MS.gene79719:CDS | 40.0% | |
| GAAAATCCTATTCCACGCAA+GGG | - | chr8.4:67826089-67826108 | None:intergenic | 40.0% | |
| GAGACTAATTGCTGTCAATG+AGG | - | chr8.4:67825739-67825758 | None:intergenic | 40.0% | |
| GAGTCTTGAAAAGGTTGACA+TGG | + | chr8.4:67825299-67825318 | MS.gene79719:CDS | 40.0% | |
| GAGTTATTCAAGTCTCACAG+AGG | + | chr8.4:67826401-67826420 | MS.gene79719:CDS | 40.0% | |
| GCCTTAGCAACCTATGAATA+TGG | - | chr8.4:67825554-67825573 | None:intergenic | 40.0% | |
| TATATCTGGATGGCAAACAG+TGG | + | chr8.4:67826232-67826251 | MS.gene79719:CDS | 40.0% | |
| TCTTGTAAACTTAGCCCAGA+AGG | + | chr8.4:67825987-67826006 | MS.gene79719:CDS | 40.0% | |
| TGGGCTAAGTTTACAAGACT+CGG | - | chr8.4:67825985-67826004 | None:intergenic | 40.0% | |
| TGTCATGAGGTGAGTATATC+TGG | + | chr8.4:67826218-67826237 | MS.gene79719:CDS | 40.0% | |
| TTCAAGTAGTGTACATCCCT+TGG | - | chr8.4:67826180-67826199 | None:intergenic | 40.0% | |
| TTGAACCGTAGCTTAGCAAA+CGG | - | chr8.4:67825641-67825660 | None:intergenic | 40.0% | |
| TTGTAAACTTAGCCCAGAAG+GGG | + | chr8.4:67825989-67826008 | MS.gene79719:CDS | 40.0% | |
| ! | ATAAGTGGTGTTGGATTCGA+AGG | + | chr8.4:67825930-67825949 | MS.gene79719:CDS | 40.0% |
| ! | CGTACTGATCGTGAATCATT+TGG | + | chr8.4:67825405-67825424 | MS.gene79719:CDS | 40.0% |
| ! | TAAGTGGTGTTGGATTCGAA+GGG | + | chr8.4:67825931-67825950 | MS.gene79719:CDS | 40.0% |
| !! | TTGCTTAAGTTGGTCACCTT+CGG | + | chr8.4:67826058-67826077 | MS.gene79719:CDS | 40.0% |
| !!! | GTTTAGGATTTTGAGGCTTG+AGG | - | chr8.4:67826111-67826130 | None:intergenic | 40.0% |
| AAGACCCTTAGCAGACAAAG+AGG | - | chr8.4:67825517-67825536 | None:intergenic | 45.0% | |
| ACTCAAATTGCAGAGTCCGA+CGG | - | chr8.4:67825470-67825489 | None:intergenic | 45.0% | |
| ATCTGGTAAGTGCATGATGG+AGG | - | chr8.4:67825349-67825368 | None:intergenic | 45.0% | |
| CAAATCTCTCGTACACGTAG+AGG | + | chr8.4:67825959-67825978 | MS.gene79719:CDS | 45.0% | |
| CAGAGGCGATGTTTGCATAA+AGG | + | chr8.4:67826418-67826437 | MS.gene79719:CDS | 45.0% | |
| CCTTAGCAGACAAAGAGGAA+GGG | - | chr8.4:67825512-67825531 | None:intergenic | 45.0% | |
| CCTTCCTCTTTGTCTGCTAA+GGG | + | chr8.4:67825510-67825529 | MS.gene79719:CDS | 45.0% | |
| CTCTACGTGTACGAGAGATT+TGG | - | chr8.4:67825961-67825980 | None:intergenic | 45.0% | |
| GGAAAATCCTATTCCACGCA+AGG | - | chr8.4:67826090-67826109 | None:intergenic | 45.0% | |
| GTCATCTGGTAAGTGCATGA+TGG | - | chr8.4:67825352-67825371 | None:intergenic | 45.0% | |
| TATCTGGATGGCAAACAGTG+GGG | + | chr8.4:67826234-67826253 | MS.gene79719:CDS | 45.0% | |
| TGAACCGTAGCTTAGCAAAC+GGG | - | chr8.4:67825640-67825659 | None:intergenic | 45.0% | |
| TGCTCACAGATATCCGACAA+AGG | + | chr8.4:67825843-67825862 | MS.gene79719:CDS | 45.0% | |
| ! | CTAACCCGTTTGCTAAGCTA+CGG | + | chr8.4:67825633-67825652 | MS.gene79719:CDS | 45.0% |
| ! | GAGTCCGACGGTTGAAATTT+TGG | - | chr8.4:67825458-67825477 | None:intergenic | 45.0% |
| !! | TGCTTAAGTTGGTCACCTTC+GGG | + | chr8.4:67826059-67826078 | MS.gene79719:CDS | 45.0% |
| AGGGACAACCAGAAGCAACT+AGG | - | chr8.4:67825719-67825738 | None:intergenic | 50.0% | |
| CCCTTAGCAGACAAAGAGGA+AGG | - | chr8.4:67825513-67825532 | None:intergenic | 50.0% | |
| CCCTTCCTCTTTGTCTGCTA+AGG | + | chr8.4:67825509-67825528 | MS.gene79719:CDS | 50.0% | |
| GATGGAGGTAGCACCAAGTT+TGG | - | chr8.4:67825334-67825353 | None:intergenic | 50.0% | |
| GGGACAACCAGAAGCAACTA+GGG | - | chr8.4:67825718-67825737 | None:intergenic | 50.0% | |
| TCACACTGCGAGCGTATAAG+TGG | + | chr8.4:67825915-67825934 | MS.gene79719:CDS | 50.0% | |
| TGCGAGCGTATAAGTGGTGT+TGG | + | chr8.4:67825921-67825940 | MS.gene79719:CDS | 50.0% | |
| TTTGGATATGCAGCCATCGG+TGG | - | chr8.4:67825440-67825459 | None:intergenic | 50.0% | |
| ACAGTTCCGATCACCCCTTC+TGG | - | chr8.4:67826005-67826024 | None:intergenic | 55.0% | |
| ATCGGAACTGTTAGCGGTGG+CGG | + | chr8.4:67826014-67826033 | MS.gene79719:CDS | 55.0% | |
| CAGTTCCGATCACCCCTTCT+GGG | - | chr8.4:67826004-67826023 | None:intergenic | 55.0% | |
| CTTAGCCCAGAAGGGGTGAT+CGG | + | chr8.4:67825996-67826015 | MS.gene79719:CDS | 55.0% | |
| GGAGATCCCTTGCGTGGAAT+AGG | + | chr8.4:67826080-67826099 | MS.gene79719:CDS | 55.0% | |
| GGGCTATCCCTAGTTGCTTC+TGG | + | chr8.4:67825708-67825727 | MS.gene79719:CDS | 55.0% | |
| GGGGTGATCGGAACTGTTAG+CGG | + | chr8.4:67826008-67826027 | MS.gene79719:CDS | 55.0% | |
| GTGATCGGAACTGTTAGCGG+TGG | + | chr8.4:67826011-67826030 | MS.gene79719:CDS | 55.0% | |
| TGCACGGAGCTCGATGATTC+AGG | + | chr8.4:67825609-67825628 | MS.gene79719:CDS | 55.0% | |
| !! | TTGGCTTGACTTGCCACCGA+TGG | + | chr8.4:67825424-67825443 | MS.gene79719:CDS | 55.0% |
| GGCTTGCGAGCGTTACGAGA+CGG | + | chr8.4:67826317-67826336 | MS.gene79719:CDS | 60.0% | |
| CCACGCAAGGGATCTCCCGA+AGG | - | chr8.4:67826077-67826096 | None:intergenic | 65.0% | |
| CCTTCGGGAGATCCCTTGCG+TGG | + | chr8.4:67826074-67826093 | MS.gene79719:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 67825297 | 67826484 | 67825297 | ID=MS.gene79719 |
| chr8.4 | mRNA | 67825297 | 67826484 | 67825297 | ID=MS.gene79719.t1;Parent=MS.gene79719 |
| chr8.4 | exon | 67825297 | 67826484 | 67825297 | ID=MS.gene79719.t1.exon1;Parent=MS.gene79719.t1 |
| chr8.4 | CDS | 67825297 | 67826484 | 67825297 | ID=cds.MS.gene79719.t1;Parent=MS.gene79719.t1 |
| Gene Sequence |
| Protein sequence |
