Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92141.t1 | XP_013444708.1 | 98.2 | 395 | 7 | 0 | 1 | 395 | 1 | 395 | 3.00E-97 | 365.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92141.t1 | Q9SRR1 | 49.8 | 295 | 142 | 3 | 8 | 297 | 1 | 294 | 1.3e-40 | 168.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92141.t1 | A0A072TPF2 | 98.2 | 395 | 7 | 0 | 1 | 395 | 1 | 395 | 2.2e-97 | 365.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene92141 | MS.gene64806 | PPI |
| MS.gene92141 | MS.gene040706 | PPI |
| MS.gene92141 | MS.gene073674 | PPI |
| MS.gene92141 | MS.gene60611 | PPI |
| MS.gene92141 | MS.gene02998 | PPI |
| MS.gene92141 | MS.gene068164 | PPI |
| MS.gene92141 | MS.gene59505 | PPI |
| MS.gene92141 | MS.gene67485 | PPI |
| MS.gene92141 | MS.gene005659 | PPI |
| MS.gene92141 | MS.gene84072 | PPI |
| MS.gene92141 | MS.gene068166 | PPI |
| MS.gene92141 | MS.gene07153 | PPI |
| MS.gene92141 | MS.gene040704 | PPI |
| MS.gene92141 | MS.gene21545 | PPI |
| MS.gene92141 | MS.gene84069 | PPI |
| MS.gene92141 | MS.gene04676 | PPI |
| MS.gene92141 | MS.gene073670 | PPI |
| MS.gene92141 | MS.gene52721 | PPI |
| MS.gene92141 | MS.gene055944 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92141.t1 | MTR_8g028140 | 97.722 | 395 | 9 | 0 | 1 | 395 | 1 | 395 | 0.0 | 777 |
| MS.gene92141.t1 | MTR_8g028140 | 97.078 | 308 | 9 | 0 | 88 | 395 | 8 | 315 | 0.0 | 598 |
| MS.gene92141.t1 | MTR_2g021030 | 28.460 | 383 | 235 | 10 | 22 | 368 | 11 | 390 | 1.01e-30 | 122 |
| MS.gene92141.t1 | MTR_2g021030 | 28.115 | 313 | 165 | 9 | 52 | 334 | 152 | 434 | 5.16e-25 | 106 |
| MS.gene92141.t1 | MTR_2g021030 | 28.460 | 383 | 235 | 10 | 22 | 368 | 11 | 390 | 2.58e-30 | 122 |
| MS.gene92141.t1 | MTR_2g021030 | 26.558 | 369 | 192 | 9 | 52 | 368 | 152 | 493 | 2.61e-23 | 102 |
| MS.gene92141.t1 | MTR_2g021030 | 29.588 | 267 | 169 | 9 | 75 | 333 | 333 | 588 | 6.50e-20 | 92.0 |
| MS.gene92141.t1 | MTR_2g021030 | 27.839 | 273 | 191 | 4 | 97 | 364 | 296 | 567 | 4.34e-18 | 86.7 |
| MS.gene92141.t1 | MTR_2g021030 | 26.515 | 264 | 186 | 5 | 95 | 352 | 320 | 581 | 2.83e-16 | 80.9 |
| MS.gene92141.t1 | MTR_1g114610 | 28.693 | 352 | 211 | 8 | 22 | 368 | 20 | 336 | 3.19e-30 | 120 |
| MS.gene92141.t1 | MTR_1g012520 | 27.968 | 379 | 242 | 7 | 7 | 368 | 53 | 417 | 2.71e-27 | 114 |
| MS.gene92141.t1 | MTR_1g012520 | 27.000 | 300 | 188 | 7 | 82 | 374 | 360 | 635 | 2.06e-17 | 84.7 |
| MS.gene92141.t1 | MTR_1g012520 | 23.118 | 372 | 216 | 9 | 66 | 374 | 193 | 557 | 2.33e-14 | 75.1 |
| MS.gene92141.t1 | MTR_7g112570 | 25.701 | 428 | 225 | 14 | 21 | 385 | 48 | 445 | 3.67e-23 | 102 |
| MS.gene92141.t1 | MTR_7g112570 | 34.459 | 148 | 71 | 2 | 97 | 219 | 442 | 588 | 2.36e-18 | 87.4 |
| MS.gene92141.t1 | MTR_7g112570 | 27.273 | 253 | 156 | 6 | 95 | 345 | 363 | 589 | 1.98e-15 | 78.6 |
| MS.gene92141.t1 | MTR_7g112570 | 23.636 | 275 | 181 | 6 | 91 | 363 | 334 | 581 | 5.08e-14 | 73.9 |
| MS.gene92141.t1 | MTR_7g112570 | 25.701 | 428 | 225 | 14 | 21 | 385 | 48 | 445 | 4.11e-23 | 101 |
| MS.gene92141.t1 | MTR_7g112570 | 34.459 | 148 | 71 | 2 | 97 | 219 | 442 | 588 | 2.39e-18 | 87.4 |
| MS.gene92141.t1 | MTR_7g112570 | 27.273 | 253 | 156 | 6 | 95 | 345 | 363 | 589 | 2.01e-15 | 78.6 |
| MS.gene92141.t1 | MTR_7g112570 | 23.636 | 275 | 181 | 6 | 91 | 363 | 334 | 581 | 5.16e-14 | 73.9 |
| MS.gene92141.t1 | MTR_7g112570 | 25.701 | 428 | 225 | 14 | 21 | 385 | 48 | 445 | 4.16e-23 | 102 |
| MS.gene92141.t1 | MTR_7g112570 | 32.934 | 167 | 83 | 3 | 97 | 235 | 442 | 607 | 3.69e-19 | 90.1 |
| MS.gene92141.t1 | MTR_7g112570 | 26.855 | 283 | 194 | 9 | 95 | 370 | 363 | 639 | 8.39e-17 | 82.8 |
| MS.gene92141.t1 | MTR_3g107280 | 27.273 | 374 | 242 | 8 | 17 | 376 | 66 | 423 | 1.71e-21 | 97.1 |
| MS.gene92141.t1 | MTR_3g107280 | 22.131 | 366 | 215 | 9 | 66 | 368 | 191 | 549 | 1.11e-12 | 70.1 |
| MS.gene92141.t1 | MTR_4g114640 | 26.685 | 356 | 240 | 8 | 18 | 364 | 60 | 403 | 3.47e-20 | 92.8 |
| MS.gene92141.t1 | MTR_4g013255 | 27.124 | 306 | 166 | 10 | 93 | 368 | 227 | 505 | 1.44e-18 | 88.2 |
| MS.gene92141.t1 | MTR_4g013255 | 25.092 | 271 | 194 | 7 | 97 | 361 | 335 | 602 | 4.75e-15 | 77.4 |
| MS.gene92141.t1 | MTR_4g013255 | 29.918 | 244 | 139 | 11 | 92 | 331 | 408 | 623 | 1.73e-14 | 75.5 |
| MS.gene92141.t1 | MTR_1g054625 | 25.571 | 219 | 154 | 3 | 91 | 301 | 75 | 292 | 4.22e-17 | 82.4 |
| MS.gene92141.t1 | MTR_1g018790 | 26.496 | 351 | 178 | 14 | 19 | 359 | 61 | 341 | 2.85e-16 | 80.9 |
| MS.gene92141.t1 | MTR_1g054625 | 27.174 | 184 | 129 | 2 | 122 | 301 | 13 | 195 | 2.48e-15 | 75.5 |
| MS.gene92141.t1 | MTR_1g054625 | 28.947 | 152 | 98 | 4 | 92 | 241 | 61 | 204 | 6.06e-11 | 62.8 |
| MS.gene92141.t1 | MTR_3g088895 | 35.922 | 103 | 65 | 1 | 95 | 196 | 180 | 282 | 7.06e-12 | 66.6 |
| MS.gene92141.t1 | MTR_1g029500 | 30.000 | 160 | 92 | 4 | 73 | 219 | 152 | 304 | 1.28e-11 | 65.9 |
| MS.gene92141.t1 | MTR_4g114650 | 24.363 | 353 | 223 | 10 | 18 | 364 | 55 | 369 | 2.36e-11 | 65.9 |
| MS.gene92141.t1 | MTR_4g114650 | 24.363 | 353 | 223 | 10 | 18 | 364 | 110 | 424 | 2.73e-11 | 65.9 |
| MS.gene92141.t1 | MTR_8g076010 | 22.963 | 405 | 249 | 13 | 20 | 385 | 13 | 393 | 2.99e-11 | 65.1 |
| MS.gene92141.t1 | MTR_4g114650 | 24.363 | 353 | 223 | 10 | 18 | 364 | 59 | 373 | 3.18e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92141.t1 | AT3G07550 | 58.763 | 388 | 154 | 3 | 8 | 390 | 1 | 387 | 1.05e-150 | 432 |
| MS.gene92141.t1 | AT3G07550 | 58.763 | 388 | 154 | 3 | 8 | 390 | 1 | 387 | 1.05e-150 | 432 |
| MS.gene92141.t1 | AT3G07550 | 54.381 | 388 | 144 | 4 | 8 | 390 | 1 | 360 | 2.51e-131 | 382 |
| MS.gene92141.t1 | AT5G23340 | 28.649 | 370 | 237 | 6 | 22 | 366 | 10 | 377 | 4.57e-35 | 134 |
| MS.gene92141.t1 | AT4G15475 | 27.818 | 417 | 220 | 13 | 22 | 368 | 11 | 416 | 5.29e-29 | 119 |
| MS.gene92141.t1 | AT4G15475 | 28.000 | 275 | 188 | 5 | 97 | 364 | 296 | 567 | 4.97e-22 | 98.6 |
| MS.gene92141.t1 | AT4G15475 | 27.099 | 262 | 166 | 5 | 108 | 368 | 256 | 493 | 2.07e-19 | 90.5 |
| MS.gene92141.t1 | AT4G15475 | 28.417 | 278 | 168 | 7 | 95 | 368 | 218 | 468 | 1.94e-18 | 87.8 |
| MS.gene92141.t1 | AT4G15475 | 32.090 | 134 | 90 | 1 | 95 | 228 | 450 | 582 | 2.32e-13 | 72.0 |
| MS.gene92141.t1 | AT2G25490 | 26.575 | 365 | 234 | 6 | 17 | 368 | 62 | 405 | 8.73e-23 | 100 |
| MS.gene92141.t1 | AT2G25490 | 27.869 | 244 | 144 | 9 | 108 | 345 | 374 | 591 | 5.86e-12 | 67.8 |
| MS.gene92141.t1 | AT5G25350 | 26.171 | 363 | 243 | 7 | 17 | 371 | 53 | 398 | 3.45e-22 | 99.0 |
| MS.gene92141.t1 | AT3G58530 | 26.337 | 243 | 155 | 4 | 122 | 363 | 112 | 331 | 6.79e-18 | 84.7 |
| MS.gene92141.t1 | AT3G58530 | 27.811 | 169 | 115 | 4 | 87 | 255 | 155 | 316 | 3.13e-11 | 64.7 |
| MS.gene92141.t1 | AT5G27920 | 27.083 | 336 | 201 | 10 | 66 | 369 | 99 | 422 | 2.99e-17 | 84.0 |
| MS.gene92141.t1 | AT5G27920 | 25.496 | 353 | 181 | 7 | 78 | 373 | 201 | 528 | 8.08e-17 | 82.8 |
| MS.gene92141.t1 | AT5G27920 | 26.523 | 279 | 191 | 8 | 100 | 370 | 330 | 602 | 4.50e-16 | 80.5 |
| MS.gene92141.t1 | AT5G27920 | 29.787 | 235 | 129 | 9 | 97 | 325 | 405 | 609 | 5.98e-14 | 73.9 |
| MS.gene92141.t1 | AT5G01720 | 25.658 | 304 | 214 | 7 | 90 | 385 | 273 | 572 | 5.77e-16 | 80.1 |
| MS.gene92141.t1 | AT5G01720 | 23.853 | 327 | 167 | 8 | 122 | 368 | 151 | 475 | 2.08e-13 | 72.4 |
| MS.gene92141.t1 | AT1G21410 | 23.885 | 314 | 165 | 9 | 90 | 376 | 85 | 351 | 1.44e-15 | 77.8 |
| MS.gene92141.t1 | AT1G21410 | 27.128 | 188 | 132 | 3 | 77 | 262 | 125 | 309 | 3.78e-13 | 70.5 |
| MS.gene92141.t1 | AT1G21410 | 23.885 | 314 | 165 | 9 | 90 | 376 | 85 | 351 | 1.44e-15 | 77.8 |
| MS.gene92141.t1 | AT1G21410 | 27.128 | 188 | 132 | 3 | 77 | 262 | 125 | 309 | 3.78e-13 | 70.5 |
| MS.gene92141.t1 | AT1G77000 | 35.714 | 112 | 71 | 1 | 107 | 217 | 103 | 214 | 5.03e-13 | 68.9 |
| MS.gene92141.t1 | AT1G77000 | 35.714 | 112 | 71 | 1 | 107 | 217 | 103 | 214 | 5.03e-13 | 68.9 |
| MS.gene92141.t1 | AT1G77000 | 35.714 | 112 | 71 | 1 | 107 | 217 | 103 | 214 | 7.23e-13 | 69.7 |
| MS.gene92141.t1 | AT1G77000 | 35.043 | 117 | 72 | 2 | 81 | 196 | 158 | 271 | 8.45e-13 | 69.7 |
| MS.gene92141.t1 | AT1G77000 | 27.528 | 178 | 124 | 3 | 87 | 262 | 135 | 309 | 1.56e-12 | 68.6 |
| MS.gene92141.t1 | AT1G77000 | 35.714 | 112 | 71 | 1 | 107 | 217 | 103 | 214 | 7.23e-13 | 69.7 |
| MS.gene92141.t1 | AT1G77000 | 35.043 | 117 | 72 | 2 | 81 | 196 | 158 | 271 | 8.45e-13 | 69.7 |
| MS.gene92141.t1 | AT1G77000 | 27.528 | 178 | 124 | 3 | 87 | 262 | 135 | 309 | 1.56e-12 | 68.6 |
| MS.gene92141.t1 | AT1G47056 | 24.302 | 358 | 216 | 12 | 22 | 370 | 43 | 354 | 6.05e-11 | 64.3 |
| MS.gene92141.t1 | AT3G50080 | 25.278 | 360 | 190 | 12 | 21 | 359 | 43 | 344 | 6.53e-11 | 64.3 |
Find 90 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGTCCGACGGTTGAAATTT+TGG | 0.184394 | 8.3:-65515119 | None:intergenic |
| ACAGTTCCGATCACCCCTTC+TGG | 0.222734 | 8.3:-65515666 | None:intergenic |
| CATGCAGTCTCTTCAAATTC+TGG | 0.257571 | 8.3:-65515929 | None:intergenic |
| TGCCTCAATCTTACTGATAA+TGG | 0.307545 | 8.3:+65515960 | MS.gene92141:CDS |
| TTGCTTAAGTTGGTCACCTT+CGG | 0.318059 | 8.3:+65515722 | MS.gene92141:CDS |
| GGGCTATCCCTAGTTGCTTC+TGG | 0.330852 | 8.3:+65515372 | MS.gene92141:CDS |
| TGCTTAAGTTGGTCACCTTC+GGG | 0.345625 | 8.3:+65515723 | MS.gene92141:CDS |
| TGTCATGAGGTGAGTATATC+TGG | 0.346053 | 8.3:+65515882 | MS.gene92141:CDS |
| CCCTTCCTCTTTGTCTGCTA+AGG | 0.349759 | 8.3:+65515173 | MS.gene92141:CDS |
| TTTACACCGTCCATATTCAT+AGG | 0.354668 | 8.3:+65515205 | MS.gene92141:CDS |
| GGAGATCCCTTGCGTGGAAT+AGG | 0.355838 | 8.3:+65515744 | MS.gene92141:CDS |
| TGCACGGAGCTCGATGATTC+AGG | 0.355920 | 8.3:+65515273 | MS.gene92141:CDS |
| CGTACTGATCGTGAATCATT+TGG | 0.356173 | 8.3:+65515069 | MS.gene92141:CDS |
| GCCTTAGCAACCTATGAATA+TGG | 0.358247 | 8.3:-65515215 | None:intergenic |
| GATGGAGGTAGCACCAAGTT+TGG | 0.367641 | 8.3:-65514995 | None:intergenic |
| ACAACCATTCAAGTACAAAA+TGG | 0.391558 | 8.3:-65516018 | None:intergenic |
| ATCTACACATTCGAAAGTTT+AGG | 0.413893 | 8.3:-65515788 | None:intergenic |
| TGTCTTAACATTACTGATGA+AGG | 0.429447 | 8.3:+65515429 | MS.gene92141:CDS |
| CAGAGGCGATGTTTGCATAA+AGG | 0.431376 | 8.3:+65516082 | MS.gene92141:CDS |
| AAGAGCTTTCAATCCTTTGT+CGG | 0.436470 | 8.3:-65515520 | None:intergenic |
| TCAGCCAATATCATCTGAAT+GGG | 0.438144 | 8.3:-65516136 | None:intergenic |
| CTCTACGTGTACGAGAGATT+TGG | 0.439223 | 8.3:-65515622 | None:intergenic |
| CAGTTCCGATCACCCCTTCT+GGG | 0.444359 | 8.3:-65515665 | None:intergenic |
| ATCGGAACTGTTAGCGGTGG+CGG | 0.448531 | 8.3:+65515678 | MS.gene92141:CDS |
| ATGGAAGATCTTTCCAAACT+TGG | 0.457693 | 8.3:+65514982 | MS.gene92141:CDS |
| TCTTCAGATGAGTCTTGAAA+AGG | 0.458770 | 8.3:+65514954 | None:intergenic |
| TAGATGTATCTTGCTTAAGT+TGG | 0.458772 | 8.3:+65515712 | MS.gene92141:CDS |
| ATCAGCCAATATCATCTGAA+TGG | 0.461278 | 8.3:-65516137 | None:intergenic |
| TATCATCTGAATGGGGAGTA+AGG | 0.462310 | 8.3:-65516128 | None:intergenic |
| TTGGCTTGACTTGCCGCCGA+TGG | 0.467505 | 8.3:+65515088 | MS.gene92141:CDS |
| TTCAAGTAGTGTACATCCCT+TGG | 0.473496 | 8.3:-65515841 | None:intergenic |
| TTGAATAACTCTAATGCTAA+AGG | 0.480544 | 8.3:-65516053 | None:intergenic |
| TGAACCGTAGCTTAGCAAAC+GGG | 0.481477 | 8.3:-65515301 | None:intergenic |
| CCTTCCTCTTTGTCTGCTAA+GGG | 0.491175 | 8.3:+65515174 | MS.gene92141:CDS |
| TCACACTGCGAGCGTATAAG+TGG | 0.492469 | 8.3:+65515579 | MS.gene92141:CDS |
| TTGAACCGTAGCTTAGCAAA+CGG | 0.494178 | 8.3:-65515302 | None:intergenic |
| GATGTACACTACTTGAAGAA+TGG | 0.498471 | 8.3:+65515847 | MS.gene92141:CDS |
| ATAATAGTAAGGCAGTCATC+TGG | 0.504519 | 8.3:-65515027 | None:intergenic |
| TGTTTGAAAGTCACTAACTA+CGG | 0.506597 | 8.3:+65515351 | MS.gene92141:CDS |
| TTAGAGTCTTTATCTCTATG+TGG | 0.511065 | 8.3:+65515249 | MS.gene92141:CDS |
| TGCGAGCGTATAAGTGGTGT+TGG | 0.528442 | 8.3:+65515585 | MS.gene92141:CDS |
| TTCTATTATTGCAATAGCCA+AGG | 0.528487 | 8.3:+65515824 | MS.gene92141:CDS |
| GTTTGAAAGTCACTAACTAC+GGG | 0.539400 | 8.3:+65515352 | MS.gene92141:CDS |
| CTTAGCCCAGAAGGGGTGAT+CGG | 0.540124 | 8.3:+65515660 | MS.gene92141:CDS |
| ATAAGTGGTGTTGGATTCGA+AGG | 0.541644 | 8.3:+65515594 | MS.gene92141:CDS |
| AGGGACAACCAGAAGCAACT+AGG | 0.542948 | 8.3:-65515380 | None:intergenic |
| TGGGCTAAGTTTACAAGACT+CGG | 0.549702 | 8.3:-65515646 | None:intergenic |
| TCTTGTAAACTTAGCCCAGA+AGG | 0.553718 | 8.3:+65515651 | MS.gene92141:CDS |
| TCCATATTCATAGGTTGCTA+AGG | 0.554256 | 8.3:+65515214 | MS.gene92141:CDS |
| ATACGTTAATAGCTTGAAGC+TGG | 0.554829 | 8.3:-65515557 | None:intergenic |
| CCCTTAGCAGACAAAGAGGA+AGG | 0.557377 | 8.3:-65515174 | None:intergenic |
| GTCATCTGGTAAGTGCATGA+TGG | 0.563773 | 8.3:-65515013 | None:intergenic |
| ATATCTGGATGGCAAACAGT+GGG | 0.564668 | 8.3:+65515897 | MS.gene92141:CDS |
| GGGACAACCAGAAGCAACTA+GGG | 0.569174 | 8.3:-65515379 | None:intergenic |
| CTTGTAAACTTAGCCCAGAA+GGG | 0.572152 | 8.3:+65515652 | MS.gene92141:CDS |
| TGACAGCAATTAGTCTCTAT+CGG | 0.572523 | 8.3:+65515406 | MS.gene92141:CDS |
| TCTATTATTGCAATAGCCAA+GGG | 0.578035 | 8.3:+65515825 | MS.gene92141:CDS |
| GAGTCTTGAAAAGGTTGACA+TGG | 0.579264 | 8.3:+65514963 | MS.gene92141:CDS |
| AGCCATTATCAGTAAGATTG+AGG | 0.584084 | 8.3:-65515962 | None:intergenic |
| AGACTAATTGCTGTCAATGA+GGG | 0.588028 | 8.3:-65515399 | None:intergenic |
| AAGACCCTTAGCAGACAAAG+AGG | 0.588868 | 8.3:-65515178 | None:intergenic |
| GGAAAATCCTATTCCACGCA+AGG | 0.589659 | 8.3:-65515751 | None:intergenic |
| GGCTTGCGAGCGTTACGAGA+CGG | 0.593002 | 8.3:+65515981 | MS.gene92141:CDS |
| GGGGTGATCGGAACTGTTAG+CGG | 0.593998 | 8.3:+65515672 | MS.gene92141:CDS |
| CTAACCCGTTTGCTAAGCTA+CGG | 0.594245 | 8.3:+65515297 | MS.gene92141:CDS |
| GAGACTAATTGCTGTCAATG+AGG | 0.594543 | 8.3:-65515400 | None:intergenic |
| ATATCTGTGAGCAGTATGAC+AGG | 0.597576 | 8.3:-65515497 | None:intergenic |
| TTTATCTCTATGTGGTTGCA+CGG | 0.599367 | 8.3:+65515257 | MS.gene92141:CDS |
| CAAATCTCTCGTACACGTAG+AGG | 0.609038 | 8.3:+65515623 | MS.gene92141:CDS |
| TTTGGATATGCAGCCATCGG+CGG | 0.612978 | 8.3:-65515101 | None:intergenic |
| CCTTCGGGAGATCCCTTGCG+TGG | 0.615820 | 8.3:+65515738 | MS.gene92141:CDS |
| TTTCAAAGATAAGCAAGCAG+TGG | 0.631769 | 8.3:-65515466 | None:intergenic |
| CCTTAGCAGACAAAGAGGAA+GGG | 0.632936 | 8.3:-65515173 | None:intergenic |
| TGTTTGCATAAAGGAAGAAG+AGG | 0.634712 | 8.3:+65516091 | MS.gene92141:CDS |
| CCACGCAAGGGATCTCCCGA+AGG | 0.635892 | 8.3:-65515738 | None:intergenic |
| ATCTGGTAAGTGCATGATGG+AGG | 0.637213 | 8.3:-65515010 | None:intergenic |
| ATATCCAAAATTTCAACCGT+CGG | 0.640422 | 8.3:+65515115 | MS.gene92141:CDS |
| TAGCAACCTATGAATATGGA+CGG | 0.643940 | 8.3:-65515211 | None:intergenic |
| TTGTAAACTTAGCCCAGAAG+GGG | 0.645808 | 8.3:+65515653 | MS.gene92141:CDS |
| TATATCTGGATGGCAAACAG+TGG | 0.653962 | 8.3:+65515896 | MS.gene92141:CDS |
| GTGATCGGAACTGTTAGCGG+TGG | 0.655476 | 8.3:+65515675 | MS.gene92141:CDS |
| GAAAATCCTATTCCACGCAA+GGG | 0.659659 | 8.3:-65515750 | None:intergenic |
| GAACTTAGCATTATGTCATG+AGG | 0.663036 | 8.3:+65515869 | MS.gene92141:CDS |
| ACTCAAATTGCAGAGTCCGA+CGG | 0.672603 | 8.3:-65515131 | None:intergenic |
| TGCTCACAGATATCCGACAA+AGG | 0.684591 | 8.3:+65515507 | MS.gene92141:CDS |
| CAGCCAATATCATCTGAATG+GGG | 0.689748 | 8.3:-65516135 | None:intergenic |
| ATGAGGTGAGTATATCTGGA+TGG | 0.692395 | 8.3:+65515886 | MS.gene92141:CDS |
| TAAGTGGTGTTGGATTCGAA+GGG | 0.692526 | 8.3:+65515595 | MS.gene92141:CDS |
| TATCTGGATGGCAAACAGTG+GGG | 0.720797 | 8.3:+65515898 | MS.gene92141:CDS |
| GAGTTATTCAAGTCTCACAG+AGG | 0.740335 | 8.3:+65516065 | MS.gene92141:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTACAAAAACTTAATC+TGG | + | chr8.3:65515323-65515342 | MS.gene92141:CDS | 15.0% |
| !! | AACCATGAAAAATAATAGTA+AGG | - | chr8.3:65515041-65515060 | None:intergenic | 20.0% |
| ! | TTGAATAACTCTAATGCTAA+AGG | - | chr8.3:65516056-65516075 | None:intergenic | 25.0% |
| !!! | TGCCTTACTATTATTTTTCA+TGG | + | chr8.3:65515036-65515055 | MS.gene92141:CDS | 25.0% |
| ACAACCATTCAAGTACAAAA+TGG | - | chr8.3:65516021-65516040 | None:intergenic | 30.0% | |
| ATATCCAAAATTTCAACCGT+CGG | + | chr8.3:65515115-65515134 | MS.gene92141:CDS | 30.0% | |
| ATCTACACATTCGAAAGTTT+AGG | - | chr8.3:65515791-65515810 | None:intergenic | 30.0% | |
| TAGATGTATCTTGCTTAAGT+TGG | + | chr8.3:65515712-65515731 | MS.gene92141:CDS | 30.0% | |
| TCTATTATTGCAATAGCCAA+GGG | + | chr8.3:65515825-65515844 | MS.gene92141:CDS | 30.0% | |
| TGTCTTAACATTACTGATGA+AGG | + | chr8.3:65515429-65515448 | MS.gene92141:CDS | 30.0% | |
| TGTTTGAAAGTCACTAACTA+CGG | + | chr8.3:65515351-65515370 | MS.gene92141:CDS | 30.0% | |
| TTAGAGTCTTTATCTCTATG+TGG | + | chr8.3:65515249-65515268 | MS.gene92141:CDS | 30.0% | |
| TTCTATTATTGCAATAGCCA+AGG | + | chr8.3:65515824-65515843 | MS.gene92141:CDS | 30.0% | |
| !! | TTCGAAAGTTTAGGATTTTG+AGG | - | chr8.3:65515782-65515801 | None:intergenic | 30.0% |
| AAGAGCTTTCAATCCTTTGT+CGG | - | chr8.3:65515523-65515542 | None:intergenic | 35.0% | |
| AGACTAATTGCTGTCAATGA+GGG | - | chr8.3:65515402-65515421 | None:intergenic | 35.0% | |
| AGCCATTATCAGTAAGATTG+AGG | - | chr8.3:65515965-65515984 | None:intergenic | 35.0% | |
| ATAATAGTAAGGCAGTCATC+TGG | - | chr8.3:65515030-65515049 | None:intergenic | 35.0% | |
| ATACGTTAATAGCTTGAAGC+TGG | - | chr8.3:65515560-65515579 | None:intergenic | 35.0% | |
| ATGGAAGATCTTTCCAAACT+TGG | + | chr8.3:65514982-65515001 | MS.gene92141:CDS | 35.0% | |
| GAACTTAGCATTATGTCATG+AGG | + | chr8.3:65515869-65515888 | MS.gene92141:CDS | 35.0% | |
| GATGTACACTACTTGAAGAA+TGG | + | chr8.3:65515847-65515866 | MS.gene92141:CDS | 35.0% | |
| GTTTGAAAGTCACTAACTAC+GGG | + | chr8.3:65515352-65515371 | MS.gene92141:CDS | 35.0% | |
| TAGCAACCTATGAATATGGA+CGG | - | chr8.3:65515214-65515233 | None:intergenic | 35.0% | |
| TCCATATTCATAGGTTGCTA+AGG | + | chr8.3:65515214-65515233 | MS.gene92141:CDS | 35.0% | |
| TGCCTCAATCTTACTGATAA+TGG | + | chr8.3:65515960-65515979 | MS.gene92141:CDS | 35.0% | |
| TGTTTGCATAAAGGAAGAAG+AGG | + | chr8.3:65516091-65516110 | MS.gene92141:CDS | 35.0% | |
| TTTACACCGTCCATATTCAT+AGG | + | chr8.3:65515205-65515224 | MS.gene92141:CDS | 35.0% | |
| TTTATCTCTATGTGGTTGCA+CGG | + | chr8.3:65515257-65515276 | MS.gene92141:CDS | 35.0% | |
| TTTCAAAGATAAGCAAGCAG+TGG | - | chr8.3:65515469-65515488 | None:intergenic | 35.0% | |
| ! | AATTTTGGATATGCAGCCAT+CGG | - | chr8.3:65515107-65515126 | None:intergenic | 35.0% |
| ! | CTCTCCATTTTGTACTTGAA+TGG | + | chr8.3:65516014-65516033 | MS.gene92141:CDS | 35.0% |
| ! | TGACAGCAATTAGTCTCTAT+CGG | + | chr8.3:65515406-65515425 | MS.gene92141:CDS | 35.0% |
| ATATCTGGATGGCAAACAGT+GGG | + | chr8.3:65515897-65515916 | MS.gene92141:CDS | 40.0% | |
| ATATCTGTGAGCAGTATGAC+AGG | - | chr8.3:65515500-65515519 | None:intergenic | 40.0% | |
| ATGAGGTGAGTATATCTGGA+TGG | + | chr8.3:65515886-65515905 | MS.gene92141:CDS | 40.0% | |
| CATGCAGTCTCTTCAAATTC+TGG | - | chr8.3:65515932-65515951 | None:intergenic | 40.0% | |
| CTTGTAAACTTAGCCCAGAA+GGG | + | chr8.3:65515652-65515671 | MS.gene92141:CDS | 40.0% | |
| GAAAATCCTATTCCACGCAA+GGG | - | chr8.3:65515753-65515772 | None:intergenic | 40.0% | |
| GAGACTAATTGCTGTCAATG+AGG | - | chr8.3:65515403-65515422 | None:intergenic | 40.0% | |
| GAGTCTTGAAAAGGTTGACA+TGG | + | chr8.3:65514963-65514982 | MS.gene92141:CDS | 40.0% | |
| GAGTTATTCAAGTCTCACAG+AGG | + | chr8.3:65516065-65516084 | MS.gene92141:CDS | 40.0% | |
| GCCTTAGCAACCTATGAATA+TGG | - | chr8.3:65515218-65515237 | None:intergenic | 40.0% | |
| TATATCTGGATGGCAAACAG+TGG | + | chr8.3:65515896-65515915 | MS.gene92141:CDS | 40.0% | |
| TCTTGTAAACTTAGCCCAGA+AGG | + | chr8.3:65515651-65515670 | MS.gene92141:CDS | 40.0% | |
| TGGGCTAAGTTTACAAGACT+CGG | - | chr8.3:65515649-65515668 | None:intergenic | 40.0% | |
| TGTCATGAGGTGAGTATATC+TGG | + | chr8.3:65515882-65515901 | MS.gene92141:CDS | 40.0% | |
| TTCAAGTAGTGTACATCCCT+TGG | - | chr8.3:65515844-65515863 | None:intergenic | 40.0% | |
| TTGAACCGTAGCTTAGCAAA+CGG | - | chr8.3:65515305-65515324 | None:intergenic | 40.0% | |
| TTGTAAACTTAGCCCAGAAG+GGG | + | chr8.3:65515653-65515672 | MS.gene92141:CDS | 40.0% | |
| ! | ATAAGTGGTGTTGGATTCGA+AGG | + | chr8.3:65515594-65515613 | MS.gene92141:CDS | 40.0% |
| ! | CGTACTGATCGTGAATCATT+TGG | + | chr8.3:65515069-65515088 | MS.gene92141:CDS | 40.0% |
| ! | TAAGTGGTGTTGGATTCGAA+GGG | + | chr8.3:65515595-65515614 | MS.gene92141:CDS | 40.0% |
| !! | TTGCTTAAGTTGGTCACCTT+CGG | + | chr8.3:65515722-65515741 | MS.gene92141:CDS | 40.0% |
| !!! | GTTTAGGATTTTGAGGCTTG+AGG | - | chr8.3:65515775-65515794 | None:intergenic | 40.0% |
| AAGACCCTTAGCAGACAAAG+AGG | - | chr8.3:65515181-65515200 | None:intergenic | 45.0% | |
| ACTCAAATTGCAGAGTCCGA+CGG | - | chr8.3:65515134-65515153 | None:intergenic | 45.0% | |
| ATCTGGTAAGTGCATGATGG+AGG | - | chr8.3:65515013-65515032 | None:intergenic | 45.0% | |
| CAAATCTCTCGTACACGTAG+AGG | + | chr8.3:65515623-65515642 | MS.gene92141:CDS | 45.0% | |
| CAGAGGCGATGTTTGCATAA+AGG | + | chr8.3:65516082-65516101 | MS.gene92141:CDS | 45.0% | |
| CCTTAGCAGACAAAGAGGAA+GGG | - | chr8.3:65515176-65515195 | None:intergenic | 45.0% | |
| CCTTCCTCTTTGTCTGCTAA+GGG | + | chr8.3:65515174-65515193 | MS.gene92141:CDS | 45.0% | |
| CTCTACGTGTACGAGAGATT+TGG | - | chr8.3:65515625-65515644 | None:intergenic | 45.0% | |
| GGAAAATCCTATTCCACGCA+AGG | - | chr8.3:65515754-65515773 | None:intergenic | 45.0% | |
| GTCATCTGGTAAGTGCATGA+TGG | - | chr8.3:65515016-65515035 | None:intergenic | 45.0% | |
| TATCTGGATGGCAAACAGTG+GGG | + | chr8.3:65515898-65515917 | MS.gene92141:CDS | 45.0% | |
| TGAACCGTAGCTTAGCAAAC+GGG | - | chr8.3:65515304-65515323 | None:intergenic | 45.0% | |
| TGCTCACAGATATCCGACAA+AGG | + | chr8.3:65515507-65515526 | MS.gene92141:CDS | 45.0% | |
| ! | CTAACCCGTTTGCTAAGCTA+CGG | + | chr8.3:65515297-65515316 | MS.gene92141:CDS | 45.0% |
| ! | GAGTCCGACGGTTGAAATTT+TGG | - | chr8.3:65515122-65515141 | None:intergenic | 45.0% |
| !! | TGCTTAAGTTGGTCACCTTC+GGG | + | chr8.3:65515723-65515742 | MS.gene92141:CDS | 45.0% |
| AGGGACAACCAGAAGCAACT+AGG | - | chr8.3:65515383-65515402 | None:intergenic | 50.0% | |
| CCCTTAGCAGACAAAGAGGA+AGG | - | chr8.3:65515177-65515196 | None:intergenic | 50.0% | |
| CCCTTCCTCTTTGTCTGCTA+AGG | + | chr8.3:65515173-65515192 | MS.gene92141:CDS | 50.0% | |
| GATGGAGGTAGCACCAAGTT+TGG | - | chr8.3:65514998-65515017 | None:intergenic | 50.0% | |
| GGGACAACCAGAAGCAACTA+GGG | - | chr8.3:65515382-65515401 | None:intergenic | 50.0% | |
| TCACACTGCGAGCGTATAAG+TGG | + | chr8.3:65515579-65515598 | MS.gene92141:CDS | 50.0% | |
| TGCGAGCGTATAAGTGGTGT+TGG | + | chr8.3:65515585-65515604 | MS.gene92141:CDS | 50.0% | |
| TTTGGATATGCAGCCATCGG+CGG | - | chr8.3:65515104-65515123 | None:intergenic | 50.0% | |
| ACAGTTCCGATCACCCCTTC+TGG | - | chr8.3:65515669-65515688 | None:intergenic | 55.0% | |
| ATCGGAACTGTTAGCGGTGG+CGG | + | chr8.3:65515678-65515697 | MS.gene92141:CDS | 55.0% | |
| CAGTTCCGATCACCCCTTCT+GGG | - | chr8.3:65515668-65515687 | None:intergenic | 55.0% | |
| CTTAGCCCAGAAGGGGTGAT+CGG | + | chr8.3:65515660-65515679 | MS.gene92141:CDS | 55.0% | |
| GGAGATCCCTTGCGTGGAAT+AGG | + | chr8.3:65515744-65515763 | MS.gene92141:CDS | 55.0% | |
| GGGCTATCCCTAGTTGCTTC+TGG | + | chr8.3:65515372-65515391 | MS.gene92141:CDS | 55.0% | |
| GGGGTGATCGGAACTGTTAG+CGG | + | chr8.3:65515672-65515691 | MS.gene92141:CDS | 55.0% | |
| GTGATCGGAACTGTTAGCGG+TGG | + | chr8.3:65515675-65515694 | MS.gene92141:CDS | 55.0% | |
| TGCACGGAGCTCGATGATTC+AGG | + | chr8.3:65515273-65515292 | MS.gene92141:CDS | 55.0% | |
| GGCTTGCGAGCGTTACGAGA+CGG | + | chr8.3:65515981-65516000 | MS.gene92141:CDS | 60.0% | |
| !! | TTGGCTTGACTTGCCGCCGA+TGG | + | chr8.3:65515088-65515107 | MS.gene92141:CDS | 60.0% |
| CCACGCAAGGGATCTCCCGA+AGG | - | chr8.3:65515741-65515760 | None:intergenic | 65.0% | |
| CCTTCGGGAGATCCCTTGCG+TGG | + | chr8.3:65515738-65515757 | MS.gene92141:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 65514961 | 65516148 | 65514961 | ID=MS.gene92141 |
| chr8.3 | mRNA | 65514961 | 65516148 | 65514961 | ID=MS.gene92141.t1;Parent=MS.gene92141 |
| chr8.3 | exon | 65514961 | 65516148 | 65514961 | ID=MS.gene92141.t1.exon1;Parent=MS.gene92141.t1 |
| chr8.3 | CDS | 65514961 | 65516148 | 65514961 | ID=cds.MS.gene92141.t1;Parent=MS.gene92141.t1 |
| Gene Sequence |
| Protein sequence |
