AlfalfaGEDB | MS.gene04676

M. sativa cultivar XinJiangDaYe / MS.gene04676

KEGG: K03094
GO: GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0019538 GO:0019941 GO:0043170 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0051603 GO:0071704 GO:1901564 GO:1901575
PFAM: PF01466.22 PF03931.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04676.t1	XP_003621661.1	92.9	156	11	0	1	156	1	156	2.20E-75	291.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04676.t1	Q39255	74.2	159	35	1	4	156	2	160	3.1e-62	239.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04676.t1	G7KSZ5	92.9	156	11	0	1	156	1	156	1.6e-75	291.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene04676	MS.gene24959	PPI
MS.gene04676	MS.gene34691	PPI
MS.gene04676	MS.gene00939	PPI
MS.gene04676	MS.gene002824	PPI
MS.gene04676	MS.gene35971	PPI
MS.gene047254	MS.gene04676	PPI
MS.gene04676	MS.gene59610	PPI
MS.gene79719	MS.gene04676	PPI
MS.gene04676	MS.gene004511	PPI
MS.gene04676	MS.gene25471	PPI
MS.gene04676	MS.gene33667	PPI
MS.gene04676	MS.gene068281	PPI
MS.gene04676	MS.gene25469	PPI
MS.gene04676	MS.gene33411	PPI
MS.gene04676	MS.gene98172	PPI
MS.gene92141	MS.gene04676	PPI
MS.gene04676	MS.gene79720	PPI
MS.gene04676	MS.gene055864	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04676.t1	MTR_7g021400	92.949	156	11	0	1	156	1	156	1.02e-100	286
MS.gene04676.t1	MTR_7g094150	92.308	156	12	0	1	156	1	156	2.43e-99	282
MS.gene04676.t1	MTR_7g094170	92.308	156	12	0	1	156	1	156	5.24e-98	279
MS.gene04676.t1	MTR_7g021380	89.103	156	17	0	1	156	1	156	6.70e-94	269
MS.gene04676.t1	MTR_5g022710	83.117	154	26	0	3	156	2	155	9.54e-90	258
MS.gene04676.t1	MTR_7g094120	78.981	157	31	1	2	156	3	159	1.75e-89	258
MS.gene04676.t1	MTR_5g022730	83.117	154	26	0	3	156	2	155	2.62e-89	257
MS.gene04676.t1	MTR_4g032400	78.205	156	33	1	2	156	8	163	1.17e-85	248
MS.gene04676.t1	MTR_4g032450	74.522	157	39	1	1	156	19	175	1.12e-83	243
MS.gene04676.t1	MTR_3g110048	74.051	158	39	1	1	156	1	158	3.64e-82	239
MS.gene04676.t1	MTR_7g094280	75.949	158	36	2	1	156	1	158	3.89e-82	239
MS.gene04676.t1	MTR_8g056060	71.338	157	44	1	1	156	1	157	5.81e-77	226
MS.gene04676.t1	MTR_7g094310	70.886	158	34	3	1	156	1	148	2.10e-72	214
MS.gene04676.t1	MTR_8g468480	67.742	155	49	1	3	156	2	156	2.65e-72	214
MS.gene04676.t1	MTR_5g085030	64.935	154	53	1	3	155	2	155	2.30e-69	206
MS.gene04676.t1	MTR_4g032500	65.385	156	47	2	2	156	3	152	1.42e-67	202
MS.gene04676.t1	MTR_4g033455	64.286	154	55	0	3	156	7	160	1.74e-67	202
MS.gene04676.t1	MTR_4g032460	69.032	155	41	2	3	156	2	150	3.67e-67	201
MS.gene04676.t1	MTR_4g032510	66.879	157	44	3	2	156	3	153	6.21e-67	200
MS.gene04676.t1	MTR_4g017860	55.897	195	43	3	5	156	26	220	2.50e-64	196
MS.gene04676.t1	MTR_4g033460	64.085	142	51	0	1	142	5	146	3.07e-62	192
MS.gene04676.t1	MTR_4g033460	64.516	93	25	1	41	133	161	245	9.10e-33	116
MS.gene04676.t1	MTR_1g099870	86.275	102	12	1	57	156	2	103	5.55e-60	181
MS.gene04676.t1	MTR_5g082340	61.935	155	47	2	3	156	2	145	2.29e-59	181
MS.gene04676.t1	MTR_4g021390	58.065	155	62	1	2	156	4	155	5.68e-57	175
MS.gene04676.t1	MTR_7g094320	57.325	157	26	2	1	156	1	117	1.91e-51	160
MS.gene04676.t1	MTR_8g089730	68.293	123	22	1	34	156	1	106	1.94e-50	157
MS.gene04676.t1	MTR_4g032420	60.630	127	35	3	31	156	38	150	1.12e-46	149
MS.gene04676.t1	MTR_7g006370	57.447	141	51	2	2	142	4	135	9.07e-45	144
MS.gene04676.t1	MTR_4g032470	51.370	146	27	2	12	156	32	134	3.60e-43	139
MS.gene04676.t1	MTR_7g094410	53.846	156	30	3	1	114	1	156	3.88e-43	140
MS.gene04676.t1	MTR_4g131980	64.706	119	36	3	3	119	2	116	2.07e-41	135
MS.gene04676.t1	MTR_4g127240	66.667	102	28	3	41	140	27	124	1.48e-40	132
MS.gene04676.t1	MTR_8g056090	51.923	156	42	6	1	154	1	125	8.07e-39	128
MS.gene04676.t1	MTR_7g094360	43.085	188	54	3	1	135	1	188	2.79e-38	129
MS.gene04676.t1	MTR_4g119422	49.655	145	66	2	2	144	227	366	3.96e-35	125
MS.gene04676.t1	MTR_1g112590	36.129	155	83	2	8	146	1	155	1.47e-29	105
MS.gene04676.t1	MTR_7g071200	71.014	69	19	1	89	156	4	72	2.12e-28	100
MS.gene04676.t1	MTR_7g094350	40.000	190	56	5	1	135	1	187	6.37e-28	102
MS.gene04676.t1	MTR_2g066010	38.411	151	81	4	8	155	20	161	8.35e-26	97.4
MS.gene04676.t1	MTR_2g065720	36.709	158	88	4	1	155	30	178	7.62e-25	95.1
MS.gene04676.t1	MTR_4g032520	40.972	144	43	3	29	154	1	120	4.96e-24	91.3
MS.gene04676.t1	MTR_4g032540	66.667	63	20	1	90	151	70	132	2.84e-23	89.0
MS.gene04676.t1	MTR_5g096950	37.179	156	83	5	1	155	1	142	7.56e-22	85.9
MS.gene04676.t1	MTR_2g018980	35.099	151	83	4	8	156	3	140	1.68e-21	85.1
MS.gene04676.t1	MTR_1g112570	36.691	139	63	4	8	128	1	132	1.78e-20	85.5
MS.gene04676.t1	MTR_1g042510	33.974	156	65	4	8	146	1	135	2.96e-20	84.7
MS.gene04676.t1	MTR_5g078680	32.051	156	68	3	8	146	1	135	3.60e-19	81.3
MS.gene04676.t1	MTR_6g053620	37.143	105	60	2	43	146	56	155	7.65e-17	76.3
MS.gene04676.t1	MTR_6g053620	37.143	105	60	2	43	146	56	155	9.08e-17	75.9
MS.gene04676.t1	MTR_8g007195	33.571	140	81	4	10	143	13	146	3.30e-16	74.3
MS.gene04676.t1	MTR_7g077810	33.793	145	84	4	8	146	18	156	8.84e-16	73.2
MS.gene04676.t1	MTR_8g007205	37.000	100	56	2	49	147	58	151	2.05e-15	71.6
MS.gene04676.t1	MTR_8g007185	37.000	100	56	2	49	147	58	151	2.07e-15	71.6
MS.gene04676.t1	MTR_4g131910	40.179	112	57	2	5	112	133	238	1.65e-14	68.6
MS.gene04676.t1	MTR_2g100830	29.861	144	87	4	10	146	13	149	4.89e-14	68.2
MS.gene04676.t1	MTR_8g030670	36.364	99	56	2	49	146	64	156	9.67e-14	67.8
MS.gene04676.t1	MTR_2g100825	28.105	153	95	4	1	146	4	148	5.81e-13	65.1
MS.gene04676.t1	MTR_3g061700	34.737	95	55	2	53	146	1	89	1.56e-12	62.8
MS.gene04676.t1	MTR_7g036780	29.787	141	88	4	1	135	1	136	7.14e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene04676.t1	AT1G20140	69.939	163	42	1	1	156	1	163	6.27e-82	239
MS.gene04676.t1	AT5G42190	71.765	170	32	1	3	156	2	171	8.45e-81	236
MS.gene04676.t1	AT1G75950	74.214	159	35	1	4	156	2	160	4.35e-80	234
MS.gene04676.t1	AT2G25700	67.273	165	43	2	1	156	1	163	2.54e-77	227
MS.gene04676.t1	AT4G34210	68.182	154	45	2	4	156	2	152	8.97e-74	218
MS.gene04676.t1	AT4G34470	66.883	154	47	2	4	156	2	152	2.68e-72	214
MS.gene04676.t1	AT3G21850	67.742	155	45	3	4	156	2	153	4.60e-69	206
MS.gene04676.t1	AT3G60010	66.234	154	49	2	5	156	2	154	5.28e-67	201
MS.gene04676.t1	AT3G21860	64.935	154	50	2	4	156	2	152	7.10e-67	200
MS.gene04676.t1	AT2G03170	61.039	154	53	2	4	156	2	149	1.38e-61	187
MS.gene04676.t1	AT3G21830	61.039	154	56	2	4	156	2	152	1.10e-58	179
MS.gene04676.t1	AT2G03190	54.217	166	63	3	4	156	2	167	3.91e-57	176
MS.gene04676.t1	AT3G60020	54.487	156	59	3	7	156	4	153	8.83e-55	170
MS.gene04676.t1	AT2G03160	47.619	189	63	3	4	156	2	190	1.87e-53	168
MS.gene04676.t1	AT3G25650	57.831	166	57	4	4	156	2	167	2.34e-53	167
MS.gene04676.t1	AT2G20160	52.288	153	67	2	4	155	2	149	4.28e-51	160
MS.gene04676.t1	AT1G10230	54.839	155	67	2	4	155	2	156	4.47e-51	160
MS.gene04676.t1	AT3G21840	59.664	119	44	2	4	121	2	117	1.22e-38	128
MS.gene04676.t1	AT3G53060	60.465	86	27	2	29	113	1	80	6.67e-30	104
MS.gene04676.t1	AT3G61415	32.414	145	86	5	8	146	18	156	2.94e-14	68.9
MS.gene04676.t1	AT3G61415	32.414	145	86	5	8	146	18	156	3.01e-14	68.9
MS.gene04676.t1	AT2G45950	29.861	144	91	4	8	146	18	156	7.02e-14	68.2
MS.gene04676.t1	AT2G45950	29.861	144	91	4	8	146	18	156	7.02e-14	68.2
MS.gene04676.t1	AT2G45950	29.861	144	91	4	8	146	18	156	7.98e-14	67.8
MS.gene04676.t1	AT2G45950	29.861	144	91	4	8	146	18	156	7.98e-14	67.8
MS.gene04676.t1	AT2G45950	29.861	144	91	4	8	146	18	156	7.98e-14	67.8

Find 37 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACGTGACCGGTCAGATAT+TGG	0.310767	7.3:+16732445	MS.gene04676:CDS
CAGAAGACGATCTTAAGAAT+TGG	0.313785	7.3:+16732528	MS.gene04676:CDS
CGAATTTCTGCTTCTTCCTC+TGG	0.323200	7.3:-16732725	None:intergenic
TGACCGGTCACGTTAGGAAC+TGG	0.377814	7.3:-16732437	None:intergenic
CTTGAATATCAAAAGTCTAC+TGG	0.408664	7.3:+16732613	MS.gene04676:CDS
AGAAGACGATCTTAAGAATT+GGG	0.420571	7.3:+16732529	MS.gene04676:CDS
ACTCTTGAGAGTGATCTTCT+TGG	0.430026	7.3:-16732318	None:intergenic
AATATCTGACCGGTCACGTT+AGG	0.431469	7.3:-16732443	None:intergenic
TGATCATGTCTGCCACAGTC+TGG	0.435485	7.3:-16732646	None:intergenic
CTTGAGAGTGATCTTCTTGG+TGG	0.435670	7.3:-16732315	None:intergenic
AACCTCAAAGGTTTCGTTGT+CGG	0.443158	7.3:-16732342	None:intergenic
AGGAACTGGGATTCCGTTGT+CGG	0.448853	7.3:-16732423	None:intergenic
GATTCAAGTGCCACAGCCTC+GGG	0.453635	7.3:-16732365	None:intergenic
TTCCGACAACGAAACCTTTG+AGG	0.470820	7.3:+16732340	MS.gene04676:CDS
CGATTCAAGTGCCACAGCCT+CGG	0.485929	7.3:-16732366	None:intergenic
CATGTCTGCCACAGTCTGGC+AGG	0.496105	7.3:-16732642	None:intergenic
CTTTGAGGTTCCCGAGGCTG+TGG	0.504428	7.3:+16732355	MS.gene04676:CDS
GACCGGTCACGTTAGGAACT+GGG	0.517391	7.3:-16732436	None:intergenic
TTCTTAAGATCGTCTTCTGA+AGG	0.528136	7.3:-16732524	None:intergenic
CTGTGGCAGACATGATCAAA+GGG	0.535149	7.3:+16732651	MS.gene04676:CDS
TGAATTCGTGAATGTTGATC+AGG	0.537071	7.3:+16732556	MS.gene04676:CDS
AATTCGTAAGGAAAACCAAT+GGG	0.541324	7.3:+16732742	MS.gene04676:CDS
AATTTCTGACGAATTTGCTC+CGG	0.551209	7.3:-16732680	None:intergenic
ATCCCAGTTCCTAACGTGAC+CGG	0.555556	7.3:+16732434	MS.gene04676:CDS
TGATTCATTCAAAAGCCCAT+TGG	0.556289	7.3:-16732757	None:intergenic
CACAGCCTCGGGAACCTCAA+AGG	0.556606	7.3:-16732354	None:intergenic
CACCTTCGCCAATATCTGAC+CGG	0.557441	7.3:-16732453	None:intergenic
GAGGACAATTGCACCGACAA+CGG	0.563633	7.3:+16732410	MS.gene04676:CDS
GGAAGAAGCAGAAATTCGTA+AGG	0.575149	7.3:+16732730	MS.gene04676:CDS
GACGATCAAGCACATGATCG+AGG	0.578464	7.3:+16732391	MS.gene04676:CDS
ACTGTGGCAGACATGATCAA+AGG	0.589851	7.3:+16732650	MS.gene04676:CDS
CGAAACCTTTGAGGTTCCCG+AGG	0.621372	7.3:+16732349	MS.gene04676:CDS
AAATTCGTAAGGAAAACCAA+TGG	0.621698	7.3:+16732741	MS.gene04676:CDS
GACCGGTCAGATATTGGCGA+AGG	0.633811	7.3:+16732451	MS.gene04676:CDS
CAAGAATGATTTCACTCCAG+AGG	0.644538	7.3:+16732709	MS.gene04676:CDS
GGATCTTACCTGCCAGACTG+TGG	0.651686	7.3:+16732634	MS.gene04676:CDS
CATGATCAAAGGGAAGACAC+CGG	0.704151	7.3:+16732661	MS.gene04676:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATTCGTAAGGAAAACCAA+TGG	+	chr7.3:16732741-16732760	MS.gene04676:CDS	30.0%
	AATTCGTAAGGAAAACCAAT+GGG	+	chr7.3:16732742-16732761	MS.gene04676:CDS	30.0%
	AGAAGACGATCTTAAGAATT+GGG	+	chr7.3:16732529-16732548	MS.gene04676:CDS	30.0%
	CTTGAATATCAAAAGTCTAC+TGG	+	chr7.3:16732613-16732632	MS.gene04676:CDS	30.0%
	CAGAAGACGATCTTAAGAAT+TGG	+	chr7.3:16732528-16732547	MS.gene04676:CDS	35.0%
	TGAATTCGTGAATGTTGATC+AGG	+	chr7.3:16732556-16732575	MS.gene04676:CDS	35.0%
	TTCTTAAGATCGTCTTCTGA+AGG	-	chr7.3:16732527-16732546	None:intergenic	35.0%
!	AATTTCTGACGAATTTGCTC+CGG	-	chr7.3:16732683-16732702	None:intergenic	35.0%
!!	GTCTTCTGAAGGTTTTTCAT+CGG	-	chr7.3:16732516-16732535	None:intergenic	35.0%
	AACCTCAAAGGTTTCGTTGT+CGG	-	chr7.3:16732345-16732364	None:intergenic	40.0%
	ACTCTTGAGAGTGATCTTCT+TGG	-	chr7.3:16732321-16732340	None:intergenic	40.0%
	CAAGAATGATTTCACTCCAG+AGG	+	chr7.3:16732709-16732728	MS.gene04676:CDS	40.0%
	GGAAGAAGCAGAAATTCGTA+AGG	+	chr7.3:16732730-16732749	MS.gene04676:CDS	40.0%
	AATATCTGACCGGTCACGTT+AGG	-	chr7.3:16732446-16732465	None:intergenic	45.0%
	ACTGTGGCAGACATGATCAA+AGG	+	chr7.3:16732650-16732669	MS.gene04676:CDS	45.0%
	CATGATCAAAGGGAAGACAC+CGG	+	chr7.3:16732661-16732680	MS.gene04676:CDS	45.0%
	CGAATTTCTGCTTCTTCCTC+TGG	-	chr7.3:16732728-16732747	None:intergenic	45.0%
	CTGTGGCAGACATGATCAAA+GGG	+	chr7.3:16732651-16732670	MS.gene04676:CDS	45.0%
	CTTGAGAGTGATCTTCTTGG+TGG	-	chr7.3:16732318-16732337	None:intergenic	45.0%
	TAACGTGACCGGTCAGATAT+TGG	+	chr7.3:16732445-16732464	MS.gene04676:CDS	45.0%
	TTCCGACAACGAAACCTTTG+AGG	+	chr7.3:16732340-16732359	MS.gene04676:CDS	45.0%
!	AGGTTTTTCATCGGAACTCG+CGG	-	chr7.3:16732507-16732526	None:intergenic	45.0%
	AGGAACTGGGATTCCGTTGT+CGG	-	chr7.3:16732426-16732445	None:intergenic	50.0%
	ATCCCAGTTCCTAACGTGAC+CGG	+	chr7.3:16732434-16732453	MS.gene04676:CDS	50.0%
	CACCTTCGCCAATATCTGAC+CGG	-	chr7.3:16732456-16732475	None:intergenic	50.0%
	GACGATCAAGCACATGATCG+AGG	+	chr7.3:16732391-16732410	MS.gene04676:CDS	50.0%
	TGATCATGTCTGCCACAGTC+TGG	-	chr7.3:16732649-16732668	None:intergenic	50.0%
!	GAGGACAATTGCACCGACAA+CGG	+	chr7.3:16732410-16732429	MS.gene04676:CDS	50.0%
	CGATTCAAGTGCCACAGCCT+CGG	-	chr7.3:16732369-16732388	None:intergenic	55.0%
	GACCGGTCACGTTAGGAACT+GGG	-	chr7.3:16732439-16732458	None:intergenic	55.0%
	GACCGGTCAGATATTGGCGA+AGG	+	chr7.3:16732451-16732470	MS.gene04676:CDS	55.0%
	GATTCAAGTGCCACAGCCTC+GGG	-	chr7.3:16732368-16732387	None:intergenic	55.0%
	GGATCTTACCTGCCAGACTG+TGG	+	chr7.3:16732634-16732653	MS.gene04676:CDS	55.0%
	TGACCGGTCACGTTAGGAAC+TGG	-	chr7.3:16732440-16732459	None:intergenic	55.0%
!!	CGAAACCTTTGAGGTTCCCG+AGG	+	chr7.3:16732349-16732368	MS.gene04676:CDS	55.0%
	CACAGCCTCGGGAACCTCAA+AGG	-	chr7.3:16732357-16732376	None:intergenic	60.0%
	CATGTCTGCCACAGTCTGGC+AGG	-	chr7.3:16732645-16732664	None:intergenic	60.0%
!!	CTTTGAGGTTCCCGAGGCTG+TGG	+	chr7.3:16732355-16732374	MS.gene04676:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	16732305	16732775	16732305	ID=MS.gene04676
chr7.3	mRNA	16732305	16732775	16732305	ID=MS.gene04676.t1;Parent=MS.gene04676
chr7.3	exon	16732305	16732775	16732305	ID=MS.gene04676.t1.exon1;Parent=MS.gene04676.t1
chr7.3	CDS	16732305	16732775	16732305	ID=cds.MS.gene04676.t1;Parent=MS.gene04676.t1

Gene Sequence

>MS.gene04676

ATGTCTTCAACCACCAAGAAGATCACTCTCAAGAGTTCCGACAACGAAACCTTTGAGGTTCCCGAGGCTGTGGCACTTGAATCGCAGACGATCAAGCACATGATCGAGGACAATTGCACCGACAACGGAATCCCAGTTCCTAACGTGACCGGTCAGATATTGGCGAAGGTGATTGAATACTGTAAGAAACATGTTGATGCCGCGAGTTCCGATGAAAAACCTTCAGAAGACGATCTTAAGAATTGGGATGCTGAATTCGTGAATGTTGATCAGGCTACGCTTTTCGATCTCATTCTTGCTGCAAATTACTTGAATATCAAAAGTCTACTGGATCTTACCTGCCAGACTGTGGCAGACATGATCAAAGGGAAGACACCGGAGCAAATTCGTCAGAAATTTAACATCAAGAATGATTTCACTCCAGAGGAAGAAGCAGAAATTCGTAAGGAAAACCAATGGGCTTTTGAATGA

Protein sequence

>MS.gene04676.t1

MSSTTKKITLKSSDNETFEVPEAVALESQTIKHMIEDNCTDNGIPVPNVTGQILAKVIEYCKKHVDAASSDEKPSEDDLKNWDAEFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEQIRQKFNIKNDFTPEEEAEIRKENQWAFE