AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050215

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050215.t1 MTR_1g090380 82.353 204 8 1 28 203 28 231 1.60e-115 331
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050215.t1 AT2G38680 62.136 206 47 4 29 203 74 279 1.36e-76 234

Find 50 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAACCAATGTTCTCTTCT+TGG 0.236832 1.2:+67740957 MS.gene050215:CDS
ATGCCACATTAACAAAGTTT+TGG 0.238917 1.2:+67739394 MS.gene050215:CDS
TCCAATATTAACAGGTTCTA+AGG 0.257059 1.2:+67740323 MS.gene050215:intron
TTCATATAACTGCTGCCTTT+TGG 0.338973 1.2:-67739575 None:intergenic
CTAATGCTAACATAGCTTTA+GGG 0.371388 1.2:+67739957 MS.gene050215:intron
TCACCGAATCGTTCGTGAAC+AGG 0.376885 1.2:-67740895 None:intergenic
AAATGAGCATGCTCTTGATA+TGG 0.389669 1.2:+67740867 MS.gene050215:CDS
CCTGTATTAATATTCTCTGC+AGG 0.422018 1.2:+67740173 MS.gene050215:CDS
CCCTTGGGCTGGAAGAGAAA+AGG 0.437854 1.2:+67739626 MS.gene050215:CDS
CCTGCAGAGAATATTAATAC+AGG 0.451901 1.2:-67740173 None:intergenic
TGGTGATCACACTGGAGACT+TGG 0.455229 1.2:+67740977 MS.gene050215:CDS
GAACGATTCGGTGATATTGA+TGG 0.458869 1.2:+67740904 MS.gene050215:CDS
CTGGATTATCCTGCTGCAAA+AGG 0.467977 1.2:-67739543 None:intergenic
AACCCAAAACTTTGTTAATG+TGG 0.470399 1.2:-67739397 None:intergenic
ATGTGAAGATAGTATCCAAC+AGG 0.474848 1.2:+67740371 MS.gene050215:CDS
AGTTGGTGGTGACGATGGAT+TGG 0.487760 1.2:-67738621 None:intergenic
AGCAAGTCATTGTCATCAGT+TGG 0.490335 1.2:-67740928 None:intergenic
TGGTGACGATGGATTGGAGT+TGG 0.494711 1.2:-67738615 None:intergenic
GAGCCTGTTCACGAACGATT+CGG 0.505027 1.2:+67740892 MS.gene050215:CDS
GTTGGAGGGCGATAGCTCAT+TGG 0.511482 1.2:-67738597 None:intergenic
CACCTTTAAAAGATACAAGT+TGG 0.511666 1.2:-67740411 None:intergenic
ATCCAGAATATGATGCCAAA+AGG 0.511735 1.2:+67739560 MS.gene050215:CDS
ATTGTTAAATACCATCCTGT+TGG 0.514081 1.2:-67740386 None:intergenic
ATATGAACATTACCATCCAT+TGG 0.515894 1.2:+67739591 MS.gene050215:CDS
CTGTATTAATATTCTCTGCA+GGG 0.521088 1.2:+67740174 MS.gene050215:CDS
TGACGATGGATTGGAGTTGG+AGG 0.533565 1.2:-67738612 None:intergenic
GAGTCATTTGTAGGCAGTCA+TGG 0.540817 1.2:+67739520 MS.gene050215:intron
GGTGATCACACTGGAGACTT+GGG 0.548044 1.2:+67740978 MS.gene050215:CDS
GCTACACTTCTTCAACATCA+AGG 0.560582 1.2:+67738665 MS.gene050215:CDS
AAAGTGACGTGGGAGAAAGT+TGG 0.564371 1.2:-67738638 None:intergenic
GAGAAAGTTGGTGGTGACGA+TGG 0.566081 1.2:-67738626 None:intergenic
TAGGAGAAAATTCCAATGGA+TGG 0.567633 1.2:-67739603 None:intergenic
CATGTAACATACTTTGGCCG+CGG 0.569211 1.2:-67739428 None:intergenic
TGATGTTGAAGAAGTGTAGC+GGG 0.570667 1.2:-67738662 None:intergenic
GGAGACTTGGGAATGTCTGA+TGG 0.575829 1.2:+67740990 MS.gene050215:CDS
TCTCTTCTTGGTGATCACAC+TGG 0.576344 1.2:+67740969 MS.gene050215:CDS
GACGATGGATTGGAGTTGGA+GGG 0.605152 1.2:-67738611 None:intergenic
AAAGGAAACTCATGGTAGAG+TGG 0.614036 1.2:+67739644 MS.gene050215:CDS
TTCTCTTCCAGCCCAAGGGT+AGG 0.615641 1.2:-67739622 None:intergenic
AGGGTAGGAGAAAATTCCAA+TGG 0.624608 1.2:-67739607 None:intergenic
GGAAGAGAAAAGGAAACTCA+TGG 0.628987 1.2:+67739636 MS.gene050215:CDS
GTGACGTGGGAGAAAGTTGG+TGG 0.633859 1.2:-67738635 None:intergenic
GCTTGCTGATATCATTGAAG+AGG 0.634896 1.2:+67740196 MS.gene050215:CDS
AACAGGATGGTATTTAACAA+TGG 0.634907 1.2:+67740388 MS.gene050215:CDS
TGCTAACATAGCTTTAGGGA+AGG 0.639195 1.2:+67739961 MS.gene050215:intron
GAAGATAGTATCCAACAGGA+TGG 0.654156 1.2:+67740375 MS.gene050215:CDS
GTGTAGCGGGAAAAGTGACG+TGG 0.663698 1.2:-67738649 None:intergenic
GATCACCAAGAAGAGAACAT+TGG 0.675843 1.2:-67740962 None:intergenic
TTGATGTTGAAGAAGTGTAG+CGG 0.688175 1.2:-67738663 None:intergenic
TGTAGCGGGAAAAGTGACGT+GGG 0.689625 1.2:-67738648 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAATTTTTTTTCTTTTA+AGG + chr1.2:67738748-67738767 MS.gene050215:intron 10.0%
!!! ATTTATATTTTCTTTTTTGT+AGG + chr1.2:67739356-67739375 MS.gene050215:intron 10.0%
!!! CTGTTTTTTTTTTTTTTTAT+GGG + chr1.2:67739731-67739750 MS.gene050215:intron 10.0%
!!! TCTGTTTTTTTTTTTTTTTA+TGG + chr1.2:67739730-67739749 MS.gene050215:intron 10.0%
!!! TGTAATTTTTTTTCTTTTAA+GGG + chr1.2:67738749-67738768 MS.gene050215:intron 10.0%
!! AAAAAAAATTACATGCAATA+AGG - chr1.2:67738742-67738761 None:intergenic 15.0%
!! AAAAAAATTACATGCAATAA+GGG - chr1.2:67738741-67738760 None:intergenic 15.0%
!! ATATATTTGTCAAAGAAAAA+TGG - chr1.2:67740024-67740043 None:intergenic 15.0%
!!! AGAAAATATAAATCTAGAAT+TGG - chr1.2:67739349-67739368 None:intergenic 15.0%
!!! GAAAATATAAATCTAGAATT+GGG - chr1.2:67739348-67739367 None:intergenic 15.0%
!!! TCATCTTAAGTTTTTATATT+CGG - chr1.2:67739226-67739245 None:intergenic 15.0%
!! AAAAAATTACATGCAATAAG+GGG - chr1.2:67738740-67738759 None:intergenic 20.0%
!! AAAATAACAAAATGGGAAAA+TGG - chr1.2:67740137-67740156 None:intergenic 20.0%
!! AAACTCAATTATCAATGAAA+TGG - chr1.2:67738935-67738954 None:intergenic 20.0%
!! AACTCAATTATCAATGAAAT+GGG - chr1.2:67738934-67738953 None:intergenic 20.0%
!! AATACACAATAAATGAAAGT+TGG - chr1.2:67740448-67740467 None:intergenic 20.0%
!! CATAATAATTAATTGCCTTT+AGG + chr1.2:67740527-67740546 MS.gene050215:intron 20.0%
!! TAATTGCATAACACAAAATT+GGG + chr1.2:67738999-67739018 MS.gene050215:intron 20.0%
!! TTAATTATTATGTAATTCCC+CGG - chr1.2:67740519-67740538 None:intergenic 20.0%
!! TTCCTGAAAAATAACAAAAT+GGG - chr1.2:67740144-67740163 None:intergenic 20.0%
!! TTTCCTGAAAAATAACAAAA+TGG - chr1.2:67740145-67740164 None:intergenic 20.0%
!!! AAAGTCTATTTGTTTTATGT+TGG + chr1.2:67740224-67740243 MS.gene050215:intron 20.0%
!!! TATGCAAATATATTTTTCAG+AGG + chr1.2:67740493-67740512 MS.gene050215:intron 20.0%
!!! TGATATGTTCATTTTATATC+AGG + chr1.2:67740056-67740075 MS.gene050215:intron 20.0%
! AAAAATTACATGCAATAAGG+GGG - chr1.2:67738739-67738758 None:intergenic 25.0%
! AATAGTCATGTAACATACTT+TGG - chr1.2:67739437-67739456 None:intergenic 25.0%
! ACTCAATTATCAATGAAATG+GGG - chr1.2:67738933-67738952 None:intergenic 25.0%
! ATTTAATTATGTTTAAGCCC+TGG + chr1.2:67740597-67740616 MS.gene050215:intron 25.0%
! CAAGCATTATTATATAGTGT+GGG - chr1.2:67739105-67739124 None:intergenic 25.0%
! GTAATTGCATAACACAAAAT+TGG + chr1.2:67738998-67739017 MS.gene050215:intron 25.0%
! TCAAGCATTATTATATAGTG+TGG - chr1.2:67739106-67739125 None:intergenic 25.0%
! TGTAAAATTGAGATACTAGT+GGG + chr1.2:67739153-67739172 MS.gene050215:intron 25.0%
! TTGTAAAATTGAGATACTAG+TGG + chr1.2:67739152-67739171 MS.gene050215:intron 25.0%
! TTTATTGTGTATTTGTTTGC+TGG + chr1.2:67740455-67740474 MS.gene050215:intron 25.0%
!! ATTTCTCTTCATTTTCTTGT+CGG + chr1.2:67740262-67740281 MS.gene050215:intron 25.0%
!! GTATCTCAATTTTACAAAAG+TGG - chr1.2:67739149-67739168 None:intergenic 25.0%
!! TACTATCTTCACATTTTTGA+AGG - chr1.2:67740365-67740384 None:intergenic 25.0%
!!! ATTTTGTTGTGGAAAACATT+TGG + chr1.2:67738785-67738804 MS.gene050215:intron 25.0%
!!! TTAACAAAGTTTTGGGTTAA+TGG + chr1.2:67739402-67739421 MS.gene050215:CDS 25.0%
!!! TTCCCATTTTGTTATTTTTC+AGG + chr1.2:67740139-67740158 MS.gene050215:intron 25.0%
AAAATTACATGCAATAAGGG+GGG - chr1.2:67738738-67738757 None:intergenic 30.0%
AACAGGATGGTATTTAACAA+TGG + chr1.2:67740388-67740407 MS.gene050215:CDS 30.0%
AACCCAAAACTTTGTTAATG+TGG - chr1.2:67739400-67739419 None:intergenic 30.0%
AATTCTCAATCCCTACAAAA+CGG - chr1.2:67739321-67739340 None:intergenic 30.0%
ATATGAACATTACCATCCAT+TGG + chr1.2:67739591-67739610 MS.gene050215:CDS 30.0%
ATTGTTAAATACCATCCTGT+TGG - chr1.2:67740389-67740408 None:intergenic 30.0%
CTCCAAAAATTCAAAAAGCT+CGG - chr1.2:67739992-67740011 None:intergenic 30.0%
CTGTATTAATATTCTCTGCA+GGG + chr1.2:67740174-67740193 MS.gene050215:CDS 30.0%
GAAATGGAGGATATCAAATT+GGG + chr1.2:67738807-67738826 MS.gene050215:intron 30.0%
GATTTGTATAGGAGAAAACA+AGG - chr1.2:67740723-67740742 None:intergenic 30.0%
GTAACTAGTAAGTAAACTCT+GGG - chr1.2:67739777-67739796 None:intergenic 30.0%
TAACTAGTAAGTAAACTCTG+GGG - chr1.2:67739776-67739795 None:intergenic 30.0%
TATCTAATCGAGTCATTTGT+AGG + chr1.2:67739511-67739530 MS.gene050215:intron 30.0%
TCCAATATTAACAGGTTCTA+AGG + chr1.2:67740323-67740342 MS.gene050215:intron 30.0%
TCGAAAATCTGGATTTGTAT+AGG - chr1.2:67740734-67740753 None:intergenic 30.0%
TGATGCTGTAACTTAAGATT+TGG + chr1.2:67739853-67739872 MS.gene050215:intron 30.0%
TGTAACTAGTAAGTAAACTC+TGG - chr1.2:67739778-67739797 None:intergenic 30.0%
TTGTGGAAAACATTTGGAAA+TGG + chr1.2:67738791-67738810 MS.gene050215:intron 30.0%
! ATGCCACATTAACAAAGTTT+TGG + chr1.2:67739394-67739413 MS.gene050215:CDS 30.0%
! CACCTTTAAAAGATACAAGT+TGG - chr1.2:67740414-67740433 None:intergenic 30.0%
! CTAATGCTAACATAGCTTTA+GGG + chr1.2:67739957-67739976 MS.gene050215:intron 30.0%
! TCCTAATTTTTGACGTGTAA+GGG - chr1.2:67739479-67739498 None:intergenic 30.0%
! TCGTTTTCTACATTTTGTTG+TGG + chr1.2:67738774-67738793 MS.gene050215:intron 30.0%
! TGCCACATTAACAAAGTTTT+GGG + chr1.2:67739395-67739414 MS.gene050215:CDS 30.0%
! TGTTTAAGAGTCTCAGTTTT+GGG + chr1.2:67739186-67739205 MS.gene050215:intron 30.0%
! TTCCGAGCTTTTTGAATTTT+TGG + chr1.2:67739987-67740006 MS.gene050215:CDS 30.0%
! TTCCTAATTTTTGACGTGTA+AGG - chr1.2:67739480-67739499 None:intergenic 30.0%
!! AAATATATTTTTCAGAGGCC+GGG + chr1.2:67740498-67740517 MS.gene050215:intron 30.0%
!! CAAATATATTTTTCAGAGGC+CGG + chr1.2:67740497-67740516 MS.gene050215:intron 30.0%
!! TAAGATTTGGTGGTAGTATT+AGG + chr1.2:67739866-67739885 MS.gene050215:intron 30.0%
!! TACTTCTCAATTTTGATCAC+AGG + chr1.2:67740820-67740839 MS.gene050215:intron 30.0%
!!! CTATTTGTTTTATGTTGGCA+AGG + chr1.2:67740229-67740248 MS.gene050215:intron 30.0%
!!! TCGCTTTTTCCAATATTAAC+AGG + chr1.2:67740315-67740334 MS.gene050215:intron 30.0%
AAATGAGCATGCTCTTGATA+TGG + chr1.2:67740867-67740886 MS.gene050215:CDS 35.0%
AACGGTAAAAAAGAGAAGAG+GGG - chr1.2:67738709-67738728 None:intergenic 35.0%
AACTAGTAAGTAAACTCTGG+GGG - chr1.2:67739775-67739794 None:intergenic 35.0%
AGCAATAAGCTTCGAAAATC+TGG - chr1.2:67740745-67740764 None:intergenic 35.0%
ATCCAGAATATGATGCCAAA+AGG + chr1.2:67739560-67739579 MS.gene050215:CDS 35.0%
ATGTGAAGATAGTATCCAAC+AGG + chr1.2:67740371-67740390 MS.gene050215:CDS 35.0%
ATTGAATATCACTGTACCTC+TGG - chr1.2:67739821-67739840 None:intergenic 35.0%
CCTGCAGAGAATATTAATAC+AGG - chr1.2:67740176-67740195 None:intergenic 35.0%
CCTGTATTAATATTCTCTGC+AGG + chr1.2:67740173-67740192 MS.gene050215:CDS 35.0%
GAACGGTAAAAAAGAGAAGA+GGG - chr1.2:67738710-67738729 None:intergenic 35.0%
GCCTTAGAACCTGTTAATAT+TGG - chr1.2:67740327-67740346 None:intergenic 35.0%
GCTAATGCTAACATAGCTTT+AGG + chr1.2:67739956-67739975 MS.gene050215:intron 35.0%
GGAAATGGAGGATATCAAAT+TGG + chr1.2:67738806-67738825 MS.gene050215:intron 35.0%
GTATTACCAGTGAAACCTAA+AGG - chr1.2:67740545-67740564 None:intergenic 35.0%
TAGGAGAAAATTCCAATGGA+TGG - chr1.2:67739606-67739625 None:intergenic 35.0%
TCCCTTACACGTCAAAAATT+AGG + chr1.2:67739475-67739494 MS.gene050215:intron 35.0%
TGCTGTAACTTAAGATTTGG+TGG + chr1.2:67739856-67739875 MS.gene050215:intron 35.0%
TGGAAAACATTTGGAAATGG+AGG + chr1.2:67738794-67738813 MS.gene050215:intron 35.0%
! ACCTACAAATCCGTTTTGTA+GGG + chr1.2:67739308-67739327 MS.gene050215:intron 35.0%
! ATTTGGTGGTAGTATTAGGT+GGG + chr1.2:67739870-67739889 MS.gene050215:intron 35.0%
! CAATGCTGGTCTACATATAT+AGG + chr1.2:67739684-67739703 MS.gene050215:intron 35.0%
! GAAAAAACCTGAAGCTTTTG+AGG - chr1.2:67738907-67738926 None:intergenic 35.0%
! GGCCAACTTGTATCTTTTAA+AGG + chr1.2:67740409-67740428 MS.gene050215:CDS 35.0%
! GTGTTTAAGAGTCTCAGTTT+TGG + chr1.2:67739185-67739204 MS.gene050215:intron 35.0%
! TAATTGCCTTTAGGTTTCAC+TGG + chr1.2:67740536-67740555 MS.gene050215:intron 35.0%
! TTCATATAACTGCTGCCTTT+TGG - chr1.2:67739578-67739597 None:intergenic 35.0%
! TTGATGTTGAAGAAGTGTAG+CGG - chr1.2:67738666-67738685 None:intergenic 35.0%
!! AATATATTTTTCAGAGGCCG+GGG + chr1.2:67740499-67740518 MS.gene050215:intron 35.0%
!! CGAGCTTTTTGAATTTTTGG+AGG + chr1.2:67739990-67740009 MS.gene050215:CDS 35.0%
AAAGGAAACTCATGGTAGAG+TGG + chr1.2:67739644-67739663 MS.gene050215:CDS 40.0%
ACGCATGCTCTTCTTATTGA+AGG + chr1.2:67739898-67739917 MS.gene050215:intron 40.0%
ACGGTAAAAAAGAGAAGAGG+GGG - chr1.2:67738708-67738727 None:intergenic 40.0%
AGCAAGTCATTGTCATCAGT+TGG - chr1.2:67740931-67740950 None:intergenic 40.0%
AGGGTAGGAGAAAATTCCAA+TGG - chr1.2:67739610-67739629 None:intergenic 40.0%
CATGCTCTTCTTATTGAAGG+AGG + chr1.2:67739901-67739920 MS.gene050215:intron 40.0%
GAAGATAGTATCCAACAGGA+TGG + chr1.2:67740375-67740394 MS.gene050215:CDS 40.0%
GAAGATTCGATTGCTGACAT+TGG + chr1.2:67740108-67740127 MS.gene050215:intron 40.0%
GAGAACCAATGTTCTCTTCT+TGG + chr1.2:67740957-67740976 MS.gene050215:CDS 40.0%
GATCACCAAGAAGAGAACAT+TGG - chr1.2:67740965-67740984 None:intergenic 40.0%
GCTACACTTCTTCAACATCA+AGG + chr1.2:67738665-67738684 MS.gene050215:CDS 40.0%
GGAACGGTAAAAAAGAGAAG+AGG - chr1.2:67738711-67738730 None:intergenic 40.0%
GGAAGAGAAAAGGAAACTCA+TGG + chr1.2:67739636-67739655 MS.gene050215:CDS 40.0%
TATATAGTGTGGGACTTCTC+AGG - chr1.2:67739095-67739114 None:intergenic 40.0%
TGGTAGAGTGGTATGTGATT+CGG + chr1.2:67739656-67739675 MS.gene050215:intron 40.0%
! CACCTACAAATCCGTTTTGT+AGG + chr1.2:67739307-67739326 MS.gene050215:intron 40.0%
! GCTTGCTGATATCATTGAAG+AGG + chr1.2:67740196-67740215 MS.gene050215:CDS 40.0%
! TCCCTACAAAACGGATTTGT+AGG - chr1.2:67739312-67739331 None:intergenic 40.0%
! TGATGTTGAAGAAGTGTAGC+GGG - chr1.2:67738665-67738684 None:intergenic 40.0%
! TGCCTTTTGGCATCATATTC+TGG - chr1.2:67739565-67739584 None:intergenic 40.0%
! TGGAATTTTCTCCTACCCTT+GGG + chr1.2:67739611-67739630 MS.gene050215:CDS 40.0%
! TTGGAATTTTCTCCTACCCT+TGG + chr1.2:67739610-67739629 MS.gene050215:CDS 40.0%
! TTTGGTGGTAGTATTAGGTG+GGG + chr1.2:67739871-67739890 MS.gene050215:intron 40.0%
!! GAACGATTCGGTGATATTGA+TGG + chr1.2:67740904-67740923 MS.gene050215:CDS 40.0%
!! GAATTGCAGCAAGTTTGTTG+TGG - chr1.2:67738873-67738892 None:intergenic 40.0%
!! GATTTGGTGGTAGTATTAGG+TGG + chr1.2:67739869-67739888 MS.gene050215:intron 40.0%
!! GTTTGTTGTGGAGCAAAAAC+AGG - chr1.2:67738861-67738880 None:intergenic 40.0%
!! TGCTAACATAGCTTTAGGGA+AGG + chr1.2:67739961-67739980 MS.gene050215:intron 40.0%
!!! TAAGAGTCTCAGTTTTGGGA+GGG + chr1.2:67739190-67739209 MS.gene050215:intron 40.0%
!!! TTAAGAGTCTCAGTTTTGGG+AGG + chr1.2:67739189-67739208 MS.gene050215:intron 40.0%
!!! TTTTGGGTTAATGGAAACCG+CGG + chr1.2:67739411-67739430 MS.gene050215:CDS 40.0%
AAAGTGACGTGGGAGAAAGT+TGG - chr1.2:67738641-67738660 None:intergenic 45.0%
AGAGGGGGTGAAGAAGAAAA+AGG - chr1.2:67738693-67738712 None:intergenic 45.0%
ATTGGGTTCAGAATTGACGC+TGG + chr1.2:67738824-67738843 MS.gene050215:intron 45.0%
CATGTAACATACTTTGGCCG+CGG - chr1.2:67739431-67739450 None:intergenic 45.0%
CTGGATTATCCTGCTGCAAA+AGG - chr1.2:67739546-67739565 None:intergenic 45.0%
GAGTCATTTGTAGGCAGTCA+TGG + chr1.2:67739520-67739539 MS.gene050215:intron 45.0%
GAGTGTGACTCGATTGATCT+TGG - chr1.2:67739262-67739281 None:intergenic 45.0%
! TCACACATTTTGTGCACCAG+AGG + chr1.2:67739802-67739821 MS.gene050215:intron 45.0%
! TCTCTTCTTGGTGATCACAC+TGG + chr1.2:67740969-67740988 MS.gene050215:CDS 45.0%
!! ACGTGTAAGGGAGCATTAGA+GGG - chr1.2:67739467-67739486 None:intergenic 45.0%
!! GTGATTCGGTTTCTCAATGC+TGG + chr1.2:67739670-67739689 MS.gene050215:intron 45.0%
!! TTTTTCAGACTCACTCGCCA+GGG - chr1.2:67740617-67740636 None:intergenic 45.0%
!!! TTTTTTCAGACTCACTCGCC+AGG - chr1.2:67740618-67740637 None:intergenic 45.0%
ACACTCCAAATGTGCAGTGG+TGG + chr1.2:67739276-67739295 MS.gene050215:intron 50.0%
CACTCCAAATGTGCAGTGGT+GGG + chr1.2:67739277-67739296 MS.gene050215:intron 50.0%
GAGCCTGTTCACGAACGATT+CGG + chr1.2:67740892-67740911 MS.gene050215:CDS 50.0%
GGTGATCACACTGGAGACTT+GGG + chr1.2:67740978-67740997 MS.gene050215:CDS 50.0%
GTCACACTCCAAATGTGCAG+TGG + chr1.2:67739273-67739292 MS.gene050215:intron 50.0%
TCACCGAATCGTTCGTGAAC+AGG - chr1.2:67740898-67740917 None:intergenic 50.0%
TCAGAATTGACGCTGGTGGT+GGG + chr1.2:67738831-67738850 MS.gene050215:intron 50.0%
TGGTGATCACACTGGAGACT+TGG + chr1.2:67740977-67740996 MS.gene050215:CDS 50.0%
TTCAGAATTGACGCTGGTGG+TGG + chr1.2:67738830-67738849 MS.gene050215:intron 50.0%
! ACATGCAATAAGGGGGGTGT+AGG - chr1.2:67738732-67738751 None:intergenic 50.0%
! ATTTTCTCCTACCCTTGGGC+TGG + chr1.2:67739615-67739634 MS.gene050215:CDS 50.0%
! CAATAAGGGGGGTGTAGGAA+CGG - chr1.2:67738727-67738746 None:intergenic 50.0%
! CCTTTTCTCTTCCAGCCCAA+GGG - chr1.2:67739629-67739648 None:intergenic 50.0%
! TCCTTTTCTCTTCCAGCCCA+AGG - chr1.2:67739630-67739649 None:intergenic 50.0%
! TGTAGCGGGAAAAGTGACGT+GGG - chr1.2:67738651-67738670 None:intergenic 50.0%
! TTGAGATACTAGTGGGCGTG+AGG + chr1.2:67739160-67739179 MS.gene050215:intron 50.0%
!! AGTTGGTGGTGACGATGGAT+TGG - chr1.2:67738624-67738643 None:intergenic 50.0%
!! CGTGTAAGGGAGCATTAGAG+GGG - chr1.2:67739466-67739485 None:intergenic 50.0%
!! CTGAAGCTTTTGAGGACCAG+CGG - chr1.2:67738899-67738918 None:intergenic 50.0%
!! GACGATGGATTGGAGTTGGA+GGG - chr1.2:67738614-67738633 None:intergenic 50.0%
!! GACGTGTAAGGGAGCATTAG+AGG - chr1.2:67739468-67739487 None:intergenic 50.0%
!! GAGAAAGTTGGTGGTGACGA+TGG - chr1.2:67738629-67738648 None:intergenic 50.0%
!! TGACGATGGATTGGAGTTGG+AGG - chr1.2:67738615-67738634 None:intergenic 50.0%
!! TGGTGACGATGGATTGGAGT+TGG - chr1.2:67738618-67738637 None:intergenic 50.0%
ACTGCCCACCACTGCACATT+TGG - chr1.2:67739284-67739303 None:intergenic 55.0%
CGCTGGTCCTCAAAAGCTTC+AGG + chr1.2:67738897-67738916 MS.gene050215:intron 55.0%
CTTGCTGCAATTCGTTCCGC+TGG + chr1.2:67738880-67738899 MS.gene050215:intron 55.0%
GGGTTCAGAATTGACGCTGG+TGG + chr1.2:67738827-67738846 MS.gene050215:intron 55.0%
GTGACGTGGGAGAAAGTTGG+TGG - chr1.2:67738638-67738657 None:intergenic 55.0%
TTCTCTTCCAGCCCAAGGGT+AGG - chr1.2:67739625-67739644 None:intergenic 55.0%
! AGATACTAGTGGGCGTGAGG+TGG + chr1.2:67739163-67739182 MS.gene050215:intron 55.0%
! CAGTCATGGCCTTTTGCAGC+AGG + chr1.2:67739534-67739553 MS.gene050215:CDS 55.0%
! CCCTTGGGCTGGAAGAGAAA+AGG + chr1.2:67739626-67739645 MS.gene050215:CDS 55.0%
! GTGTAGCGGGAAAAGTGACG+TGG - chr1.2:67738652-67738671 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 67738600 67741011 67738600 ID=MS.gene050215
chr1.2 mRNA 67738600 67741011 67738600 ID=MS.gene050215.t1;Parent=MS.gene050215
chr1.2 exon 67738600 67738686 67738600 ID=MS.gene050215.t1.exon1;Parent=MS.gene050215.t1
chr1.2 CDS 67738600 67738686 67738600 ID=cds.MS.gene050215.t1;Parent=MS.gene050215.t1
chr1.2 exon 67739378 67739438 67739378 ID=MS.gene050215.t1.exon2;Parent=MS.gene050215.t1
chr1.2 CDS 67739378 67739438 67739378 ID=cds.MS.gene050215.t1;Parent=MS.gene050215.t1
chr1.2 exon 67739533 67739665 67739533 ID=MS.gene050215.t1.exon3;Parent=MS.gene050215.t1
chr1.2 CDS 67739533 67739665 67739533 ID=cds.MS.gene050215.t1;Parent=MS.gene050215.t1
chr1.2 exon 67739972 67740011 67739972 ID=MS.gene050215.t1.exon4;Parent=MS.gene050215.t1
chr1.2 CDS 67739972 67740011 67739972 ID=cds.MS.gene050215.t1;Parent=MS.gene050215.t1
chr1.2 exon 67740161 67740217 67740161 ID=MS.gene050215.t1.exon5;Parent=MS.gene050215.t1
chr1.2 CDS 67740161 67740217 67740161 ID=cds.MS.gene050215.t1;Parent=MS.gene050215.t1
chr1.2 exon 67740337 67740430 67740337 ID=MS.gene050215.t1.exon6;Parent=MS.gene050215.t1
chr1.2 CDS 67740337 67740430 67740337 ID=cds.MS.gene050215.t1;Parent=MS.gene050215.t1
chr1.2 exon 67740842 67741011 67740842 ID=MS.gene050215.t1.exon7;Parent=MS.gene050215.t1
chr1.2 CDS 67740842 67741011 67740842 ID=cds.MS.gene050215.t1;Parent=MS.gene050215.t1
Gene Sequence

>MS.gene050215

ATGAGCTATCGCCCTCCAACTCCAATCCATCGTCACCACCAACTTTCTCCCACGTCACTTTTCCCGCTACACTTCTTCAACATCAAGGTTCCTTTTTCTTCTTCACCCCCTCTTCTCTTTTTTACCGTTCCTACACCCCCCTTATTGCATGTAATTTTTTTTCTTTTAAGGGTTTCGTTTTCTACATTTTGTTGTGGAAAACATTTGGAAATGGAGGATATCAAATTGGGTTCAGAATTGACGCTGGTGGTGGGTGATCCTGTTTTTGCTCCACAACAAACTTGCTGCAATTCGTTCCGCTGGTCCTCAAAAGCTTCAGGTTTTTTCAAACCCCATTTCATTGATAATTGAGTTTCATGCAAATCAATGATTGCATTTTATTATGTGATCATTTCATTGTAATTGCATAACACAAAATTGGGTCTCATTTATACAAAAAAAAAAAACGATTTTTTTAATTCATTACTACTAGTAGTTCAATTCAATATGTTTCCTGAGAAGTCCCACACTATATAATAATGCTTGAAAATGTGTTTACTTTACAAGCCACTTTTGTAAAATTGAGATACTAGTGGGCGTGAGGTGGTGTTTAAGAGTCTCAGTTTTGGGAGGGTTGAATTATACCGAATATAAAAACTTAAGATGATATCAGAGATGATCCAAGATCAATCGAGTCACACTCCAAATGTGCAGTGGTGGGCAGTCTTCACCTACAAATCCGTTTTGTAGGGATTGAGAATTTCAACCCAATTCTAGATTTATATTTTCTTTTTTGTAGGTGATCGCTGATTTTGATGCCACATTAACAAAGTTTTGGGTTAATGGAAACCGCGGCCAAAGTATGTTACATGACTATTATATTTCCCCTCTAATGCTCCCTTACACGTCAAAAATTAGGAATATGTATTATATATCTAATCGAGTCATTTGTAGGCAGTCATGGCCTTTTGCAGCAGGATAATCCAGAATATGATGCCAAAAGGCAGCAGTTATATGAACATTACCATCCATTGGAATTTTCTCCTACCCTTGGGCTGGAAGAGAAAAGGAAACTCATGGTAGAGTGGTATGTGATTCGGTTTCTCAATGCTGGTCTACATATATAGGCTTTAACTTGAATAGTGTTGTATTCTGTTTTTTTTTTTTTTTATGGGTAGAAATCAAATTCAGTACCCCCAGAGTTTACTTACTAGTTACAACACTCACACATTTTGTGCACCAGAGGTACAGTGATATTCAATTGACATTAACTTTGATGCTGTAACTTAAGATTTGGTGGTAGTATTAGGTGGGGAAAAACGCATGCTCTTCTTATTGAAGGAGGACTTACATATGAAATCAATAAAACAATCAGTTGCTAATGCTAACATAGCTTTAGGGAAGGTGTTTCCGAGCTTTTTGAATTTTTGGAGGTATATATTCCATTTTTCTTTGACAAATATATGTTCTTACATGTTGATATGTTCATTTTATATCAGGAGAATTTATGAAATGTAATATATATGTGTGAAGATTCGATTGCTGACATTGGATACCATTTTCCCATTTTGTTATTTTTCAGGAAAGAGACATTCCTGTATTAATATTCTCTGCAGGGCTTGCTGATATCATTGAAGAGGTGTGAAAAGTCTATTTGTTTTATGTTGGCAAGGTTTACTAAGAATTTCTCTTCATTTTCTTGTCGGTTATTGATTGTGCATCTCAATATCTCTGTCTCGCTTTTTCCAATATTAACAGGTTCTAAGGCAGAAGCTTCGCAGATCCTTCAAAAATGTGAAGATAGTATCCAACAGGATGGTATTTAACAATGGCCAACTTGTATCTTTTAAAGGTGATTCTATGTTTCCAACTTTCATTTATTGTGTATTTGTTTGCTGGTCTTCTCAATGTTCATATGCAAATATATTTTTCAGAGGCCGGGGAATTACATAATAATTAATTGCCTTTAGGTTTCACTGGTAATACAGAAAAGCACACTTGTATTACAATGTTTGTTTATTTAATTATGTTTAAGCCCTGGCGAGTGAGTCTGAAAAAAAATAAACATCTGTAATTTGATAGTGAAAAGTACATTTGTATTACAATGTAGTTCTGCAATTTGATATTGACTTCACATATAGCCTTGTTTTCTCCTATACAAATCCAGATTTTCGAAGCTTATTGCTGAGTTGAAAATAAACATTTCATATGCGTACTTAACAAGAACTAGTTCTTAATTTTTACTTCTCAATTTTGATCACAGGAAAATTGATTCACAGCTTAAATAAAAATGAGCATGCTCTTGATATGGCTGAGCCTGTTCACGAACGATTCGGTGATATTGATGGTCCAACTGATGACAATGACTTGCTCAAGAAGAGAACCAATGTTCTCTTCTTGGTGATCACACTGGAGACTTGGGAATGTCTGATG
Protein sequence

>MS.gene050215.t1

MSYRPPTPIHRHHQLSPTSLFPLHFFNIKVIADFDATLTKFWVNGNRGQSSHGLLQQDNPEYDAKRQQLYEHYHPLEFSPTLGLEEKRKLMVECFREGVSELFEFLEERDIPVLIFSAGLADIIEEVLRQKLRRSFKNVKIVSNRMVFNNGQLVSFKGKLIHSLNKNEHALDMAEPVHERFGDIDGPTDDNDLLKKRTNVLFLVITLETWECLM