Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91584.t1 | XP_003621474.2 | 91.3 | 458 | 40 | 0 | 1 | 458 | 1 | 458 | 2.10E-243 | 851.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91584.t1 | Q940Z5 | 39.1 | 470 | 257 | 12 | 3 | 456 | 11 | 467 | 1.2e-85 | 318.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91584.t1 | G7L664 | 91.3 | 458 | 40 | 0 | 1 | 458 | 1 | 458 | 1.5e-243 | 851.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene91584 | 0.809301 | 2.02E-50 | -1.69E-46 |
| MS.gene049260 | MS.gene91584 | 0.808099 | 3.66E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene91584 | 0.839482 | 1.54E-57 | -1.69E-46 |
| MS.gene050128 | MS.gene91584 | 0.832585 | 8.69E-56 | -1.69E-46 |
| MS.gene050215 | MS.gene91584 | 0.821374 | 4.18E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene91584 | 0.819782 | 9.69E-53 | -1.69E-46 |
| MS.gene050397 | MS.gene91584 | 0.811149 | 8.09E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene91584 | 0.875653 | 2.60E-68 | -1.69E-46 |
| MS.gene052011 | MS.gene91584 | -0.800655 | 1.30E-48 | -1.69E-46 |
| MS.gene052178 | MS.gene91584 | 0.812553 | 4.00E-51 | -1.69E-46 |
| MS.gene052207 | MS.gene91584 | 0.813926 | 2.00E-51 | -1.69E-46 |
| MS.gene052253 | MS.gene91584 | 0.816194 | 6.27E-52 | -1.69E-46 |
| MS.gene052485 | MS.gene91584 | 0.808137 | 3.59E-50 | -1.69E-46 |
| MS.gene052917 | MS.gene91584 | -0.802697 | 4.96E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene91584 | 0.852891 | 3.43E-61 | -1.69E-46 |
| MS.gene054010 | MS.gene91584 | 0.865288 | 6.58E-65 | -1.69E-46 |
| MS.gene054011 | MS.gene91584 | 0.864742 | 9.77E-65 | -1.69E-46 |
| MS.gene054012 | MS.gene91584 | 0.861631 | 8.96E-64 | -1.69E-46 |
| MS.gene054013 | MS.gene91584 | 0.859741 | 3.35E-63 | -1.69E-46 |
| MS.gene054183 | MS.gene91584 | 0.807143 | 5.84E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene91584 | 0.832443 | 9.43E-56 | -1.69E-46 |
| MS.gene055277 | MS.gene91584 | 0.824757 | 6.79E-54 | -1.69E-46 |
| MS.gene055390 | MS.gene91584 | 0.819488 | 1.13E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene91584 | 0.811229 | 7.77E-51 | -1.69E-46 |
| MS.gene055604 | MS.gene91584 | 0.845962 | 2.93E-59 | -1.69E-46 |
| MS.gene056191 | MS.gene91584 | 0.813593 | 2.37E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene91584 | 0.810377 | 1.19E-50 | -1.69E-46 |
| MS.gene056902 | MS.gene91584 | 0.877394 | 6.51E-69 | -1.69E-46 |
| MS.gene057448 | MS.gene91584 | 0.808822 | 2.56E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene91584 | 0.808855 | 2.52E-50 | -1.69E-46 |
| MS.gene057469 | MS.gene91584 | 0.846161 | 2.58E-59 | -1.69E-46 |
| MS.gene058002 | MS.gene91584 | 0.80019 | 1.62E-48 | -1.69E-46 |
| MS.gene05811 | MS.gene91584 | 0.861502 | 9.81E-64 | -1.69E-46 |
| MS.gene05842 | MS.gene91584 | 0.811895 | 5.57E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene91584 | 0.846285 | 2.39E-59 | -1.69E-46 |
| MS.gene059294 | MS.gene91584 | 0.844013 | 9.83E-59 | -1.69E-46 |
| MS.gene059420 | MS.gene91584 | 0.812101 | 5.02E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene91584 | 0.80414 | 2.49E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene91584 | 0.834639 | 2.67E-56 | -1.69E-46 |
| MS.gene06037 | MS.gene91584 | 0.821606 | 3.69E-53 | -1.69E-46 |
| MS.gene060986 | MS.gene91584 | 0.808336 | 3.26E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene91584 | 0.821971 | 3.04E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91584.t1 | MTR_7g014330 | 91.266 | 458 | 40 | 0 | 1 | 458 | 1 | 458 | 0.0 | 868 |
| MS.gene91584.t1 | MTR_7g014250 | 88.865 | 458 | 51 | 0 | 1 | 458 | 1 | 458 | 0.0 | 844 |
| MS.gene91584.t1 | MTR_7g014230 | 89.648 | 454 | 47 | 0 | 2 | 455 | 4 | 457 | 0.0 | 841 |
| MS.gene91584.t1 | MTR_7g014360 | 81.443 | 388 | 52 | 1 | 71 | 458 | 21 | 388 | 0.0 | 644 |
| MS.gene91584.t1 | MTR_7g014310 | 75.449 | 334 | 28 | 2 | 108 | 441 | 29 | 308 | 1.67e-173 | 489 |
| MS.gene91584.t1 | MTR_6g015815 | 47.436 | 468 | 222 | 11 | 2 | 458 | 8 | 462 | 1.46e-147 | 429 |
| MS.gene91584.t1 | MTR_6g015850 | 46.781 | 466 | 223 | 12 | 3 | 458 | 9 | 459 | 3.56e-141 | 412 |
| MS.gene91584.t1 | MTR_6g015830 | 46.467 | 467 | 226 | 11 | 3 | 458 | 9 | 462 | 2.61e-140 | 410 |
| MS.gene91584.t1 | MTR_6g015320 | 45.788 | 463 | 229 | 9 | 3 | 458 | 9 | 456 | 8.99e-140 | 409 |
| MS.gene91584.t1 | MTR_6g015315 | 46.421 | 461 | 230 | 9 | 3 | 458 | 6 | 454 | 9.34e-137 | 401 |
| MS.gene91584.t1 | MTR_7g014610 | 40.842 | 475 | 256 | 11 | 3 | 458 | 9 | 477 | 1.44e-111 | 337 |
| MS.gene91584.t1 | MTR_7g014200 | 40.938 | 469 | 256 | 12 | 3 | 458 | 10 | 470 | 2.19e-110 | 334 |
| MS.gene91584.t1 | MTR_7g014160 | 41.469 | 463 | 239 | 15 | 18 | 456 | 19 | 473 | 1.39e-108 | 330 |
| MS.gene91584.t1 | MTR_7g013850 | 38.293 | 457 | 249 | 13 | 27 | 456 | 30 | 480 | 2.78e-97 | 301 |
| MS.gene91584.t1 | MTR_7g014620 | 39.150 | 447 | 235 | 10 | 22 | 456 | 11 | 432 | 1.33e-92 | 287 |
| MS.gene91584.t1 | MTR_2g089765 | 37.708 | 480 | 251 | 15 | 3 | 452 | 17 | 478 | 3.58e-84 | 267 |
| MS.gene91584.t1 | MTR_3g028210 | 34.244 | 476 | 281 | 10 | 1 | 456 | 1 | 464 | 4.01e-82 | 261 |
| MS.gene91584.t1 | MTR_7g015860 | 35.294 | 476 | 257 | 13 | 3 | 452 | 6 | 456 | 5.38e-79 | 253 |
| MS.gene91584.t1 | MTR_2g089670 | 37.220 | 446 | 252 | 13 | 22 | 452 | 41 | 473 | 9.86e-79 | 253 |
| MS.gene91584.t1 | MTR_6g015310 | 39.118 | 363 | 164 | 7 | 74 | 433 | 23 | 331 | 1.94e-78 | 247 |
| MS.gene91584.t1 | MTR_8g012750 | 34.382 | 477 | 282 | 12 | 3 | 456 | 4 | 472 | 1.79e-76 | 247 |
| MS.gene91584.t1 | MTR_3g087640 | 34.167 | 480 | 273 | 13 | 3 | 456 | 10 | 472 | 6.70e-72 | 235 |
| MS.gene91584.t1 | MTR_2g089650 | 32.645 | 484 | 270 | 16 | 3 | 458 | 10 | 465 | 7.93e-69 | 226 |
| MS.gene91584.t1 | MTR_2g089735 | 33.185 | 449 | 247 | 13 | 39 | 458 | 39 | 463 | 1.42e-67 | 223 |
| MS.gene91584.t1 | MTR_2g089755 | 32.675 | 456 | 269 | 12 | 26 | 458 | 37 | 477 | 6.64e-67 | 222 |
| MS.gene91584.t1 | MTR_7g014630 | 42.238 | 277 | 141 | 6 | 3 | 267 | 10 | 279 | 2.91e-66 | 217 |
| MS.gene91584.t1 | MTR_7g014205 | 78.626 | 131 | 17 | 1 | 295 | 425 | 1 | 120 | 1.42e-63 | 201 |
| MS.gene91584.t1 | MTR_7g014210 | 86.957 | 115 | 14 | 1 | 314 | 427 | 24 | 138 | 3.09e-61 | 197 |
| MS.gene91584.t1 | MTR_2g089665 | 30.000 | 440 | 193 | 8 | 1 | 431 | 1 | 334 | 3.56e-48 | 169 |
| MS.gene91584.t1 | MTR_7g014180 | 45.562 | 169 | 86 | 2 | 3 | 168 | 8 | 173 | 2.75e-42 | 148 |
| MS.gene91584.t1 | MTR_3g435300 | 36.861 | 274 | 145 | 7 | 3 | 262 | 4 | 263 | 4.76e-42 | 150 |
| MS.gene91584.t1 | MTR_7g014340 | 87.324 | 71 | 9 | 0 | 1 | 71 | 1 | 71 | 8.71e-36 | 128 |
| MS.gene91584.t1 | MTR_7g014670 | 37.368 | 190 | 113 | 4 | 272 | 456 | 71 | 259 | 4.25e-34 | 129 |
| MS.gene91584.t1 | MTR_7g014220 | 83.607 | 61 | 10 | 0 | 14 | 74 | 6 | 66 | 2.31e-27 | 104 |
| MS.gene91584.t1 | MTR_0190s0020 | 28.475 | 446 | 232 | 20 | 23 | 437 | 26 | 415 | 1.93e-22 | 99.8 |
| MS.gene91584.t1 | MTR_3g026310 | 27.103 | 428 | 249 | 18 | 11 | 413 | 14 | 403 | 4.29e-22 | 99.0 |
| MS.gene91584.t1 | MTR_7g085930 | 26.291 | 426 | 270 | 13 | 57 | 456 | 54 | 461 | 8.10e-21 | 95.1 |
| MS.gene91584.t1 | MTR_8g075610 | 25.128 | 390 | 225 | 17 | 42 | 409 | 30 | 374 | 1.17e-20 | 94.4 |
| MS.gene91584.t1 | MTR_3g049150 | 25.616 | 406 | 236 | 15 | 34 | 409 | 30 | 399 | 3.81e-20 | 93.2 |
| MS.gene91584.t1 | MTR_4g022825 | 26.329 | 414 | 249 | 16 | 20 | 413 | 18 | 395 | 7.56e-20 | 92.0 |
| MS.gene91584.t1 | MTR_3g026950 | 25.113 | 442 | 256 | 17 | 32 | 433 | 21 | 427 | 9.53e-20 | 91.7 |
| MS.gene91584.t1 | MTR_4g094842 | 27.078 | 421 | 250 | 18 | 26 | 427 | 27 | 409 | 1.08e-19 | 91.7 |
| MS.gene91584.t1 | MTR_3g088420 | 25.120 | 418 | 242 | 16 | 20 | 413 | 18 | 388 | 3.54e-19 | 89.7 |
| MS.gene91584.t1 | MTR_3g101930 | 28.238 | 386 | 222 | 15 | 48 | 412 | 56 | 407 | 5.07e-19 | 89.7 |
| MS.gene91584.t1 | MTR_3g101900 | 25.000 | 412 | 258 | 13 | 18 | 412 | 22 | 399 | 5.38e-19 | 89.4 |
| MS.gene91584.t1 | MTR_2g064495 | 22.695 | 423 | 283 | 14 | 42 | 452 | 39 | 429 | 6.59e-19 | 89.0 |
| MS.gene91584.t1 | MTR_4g094835 | 24.272 | 412 | 244 | 17 | 26 | 413 | 32 | 399 | 2.49e-18 | 87.4 |
| MS.gene91584.t1 | MTR_4g127650 | 24.681 | 470 | 289 | 18 | 17 | 456 | 22 | 456 | 2.92e-18 | 87.4 |
| MS.gene91584.t1 | MTR_3g098980 | 25.061 | 407 | 251 | 15 | 62 | 453 | 66 | 433 | 3.75e-18 | 86.7 |
| MS.gene91584.t1 | MTR_7g086000 | 24.375 | 480 | 308 | 15 | 4 | 456 | 6 | 457 | 3.95e-18 | 87.0 |
| MS.gene91584.t1 | MTR_2g105330 | 22.695 | 423 | 283 | 14 | 42 | 452 | 39 | 429 | 5.66e-18 | 86.3 |
| MS.gene91584.t1 | MTR_7g085990 | 26.220 | 328 | 197 | 7 | 56 | 364 | 49 | 350 | 7.69e-18 | 86.3 |
| MS.gene91584.t1 | MTR_8g079710 | 24.884 | 430 | 247 | 19 | 26 | 424 | 29 | 413 | 5.47e-17 | 83.2 |
| MS.gene91584.t1 | MTR_8g096530 | 25.070 | 355 | 230 | 12 | 62 | 410 | 59 | 383 | 6.68e-17 | 83.2 |
| MS.gene91584.t1 | MTR_4g022720 | 26.253 | 419 | 238 | 16 | 20 | 413 | 18 | 390 | 1.10e-16 | 82.4 |
| MS.gene91584.t1 | MTR_4g022800 | 28.741 | 421 | 232 | 19 | 20 | 413 | 18 | 397 | 1.24e-16 | 82.4 |
| MS.gene91584.t1 | MTR_4g022760 | 27.321 | 377 | 198 | 19 | 20 | 367 | 18 | 347 | 9.36e-15 | 76.3 |
| MS.gene91584.t1 | MTR_4g022290 | 24.880 | 418 | 246 | 16 | 20 | 413 | 24 | 397 | 9.97e-15 | 76.3 |
| MS.gene91584.t1 | MTR_7g027175 | 23.580 | 352 | 237 | 11 | 62 | 409 | 68 | 391 | 1.29e-13 | 72.8 |
| MS.gene91584.t1 | MTR_7g084940 | 24.943 | 441 | 256 | 17 | 39 | 452 | 48 | 440 | 1.40e-12 | 69.7 |
| MS.gene91584.t1 | MTR_7g084910 | 26.230 | 305 | 175 | 14 | 39 | 328 | 49 | 318 | 4.47e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91584.t1 | AT3G29635 | 41.416 | 466 | 246 | 13 | 3 | 456 | 4 | 454 | 2.66e-100 | 308 |
| MS.gene91584.t1 | AT3G29670 | 39.700 | 466 | 251 | 12 | 4 | 458 | 5 | 451 | 2.42e-98 | 303 |
| MS.gene91584.t1 | AT5G39090 | 39.520 | 458 | 253 | 12 | 5 | 456 | 8 | 447 | 2.00e-97 | 300 |
| MS.gene91584.t1 | AT5G39050 | 38.936 | 470 | 258 | 12 | 3 | 456 | 11 | 467 | 6.13e-95 | 294 |
| MS.gene91584.t1 | AT5G61160 | 37.844 | 473 | 253 | 12 | 3 | 458 | 4 | 452 | 3.99e-94 | 292 |
| MS.gene91584.t1 | AT5G39080 | 37.419 | 465 | 261 | 12 | 4 | 452 | 7 | 457 | 4.11e-94 | 292 |
| MS.gene91584.t1 | AT3G29680 | 39.636 | 439 | 237 | 12 | 24 | 452 | 23 | 443 | 1.05e-87 | 275 |
| MS.gene91584.t1 | AT3G29590 | 39.186 | 467 | 242 | 13 | 4 | 456 | 10 | 448 | 1.31e-86 | 272 |
| MS.gene91584.t1 | AT1G03940 | 34.454 | 476 | 270 | 13 | 3 | 456 | 9 | 464 | 1.09e-78 | 252 |
| MS.gene91584.t1 | AT1G03495 | 33.333 | 360 | 204 | 10 | 1 | 340 | 1 | 344 | 4.46e-55 | 187 |
| MS.gene91584.t1 | AT3G29720 | 37.681 | 207 | 109 | 5 | 99 | 299 | 26 | 218 | 9.28e-32 | 121 |
| MS.gene91584.t1 | AT5G23940 | 25.231 | 432 | 273 | 16 | 26 | 436 | 25 | 427 | 3.45e-23 | 102 |
| MS.gene91584.t1 | AT2G19070 | 22.500 | 440 | 294 | 11 | 39 | 456 | 37 | 451 | 2.56e-22 | 99.4 |
| MS.gene91584.t1 | AT5G67160 | 24.337 | 415 | 254 | 10 | 10 | 409 | 13 | 382 | 7.01e-20 | 92.0 |
| MS.gene91584.t1 | AT5G48930 | 23.348 | 454 | 303 | 16 | 9 | 448 | 4 | 426 | 2.82e-19 | 90.1 |
| MS.gene91584.t1 | AT5G67150 | 25.647 | 425 | 257 | 14 | 10 | 413 | 13 | 399 | 3.15e-19 | 90.1 |
| MS.gene91584.t1 | AT3G29690 | 34.228 | 149 | 86 | 4 | 312 | 456 | 38 | 178 | 7.29e-18 | 81.6 |
| MS.gene91584.t1 | AT3G47170 | 26.855 | 283 | 169 | 10 | 62 | 328 | 70 | 330 | 1.94e-17 | 85.1 |
| MS.gene91584.t1 | AT1G31490 | 23.223 | 422 | 268 | 13 | 5 | 409 | 1 | 383 | 1.27e-15 | 79.3 |
| MS.gene91584.t1 | AT5G07850 | 25.000 | 380 | 229 | 12 | 54 | 413 | 62 | 405 | 3.70e-15 | 77.8 |
| MS.gene91584.t1 | AT5G38130 | 25.359 | 418 | 248 | 15 | 29 | 425 | 52 | 426 | 6.65e-15 | 77.0 |
| MS.gene91584.t1 | AT1G03390 | 23.367 | 398 | 257 | 12 | 42 | 425 | 59 | 422 | 2.45e-14 | 75.5 |
| MS.gene91584.t1 | AT3G48720 | 24.507 | 355 | 230 | 13 | 62 | 409 | 61 | 384 | 3.95e-14 | 74.7 |
| MS.gene91584.t1 | AT1G28680 | 22.131 | 366 | 232 | 9 | 62 | 412 | 62 | 389 | 1.29e-13 | 73.2 |
| MS.gene91584.t1 | AT1G28680 | 22.314 | 363 | 237 | 8 | 62 | 417 | 62 | 386 | 1.39e-13 | 72.8 |
| MS.gene91584.t1 | AT5G07860 | 24.451 | 364 | 235 | 11 | 54 | 408 | 64 | 396 | 5.75e-13 | 70.9 |
| MS.gene91584.t1 | AT5G57840 | 22.126 | 461 | 298 | 15 | 16 | 454 | 15 | 436 | 1.74e-11 | 66.2 |
Find 77 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGACTCCTGCAACATATTT+TGG | 0.089197 | 7.4:+84600940 | MS.gene91584:CDS |
| GCTGATTTCAATCATCTTTC+AGG | 0.150127 | 7.4:+84600304 | MS.gene91584:CDS |
| ATTGAAAATTTCTATGATTT+TGG | 0.166142 | 7.4:-84599987 | None:intergenic |
| TGTTGAAACAAGTGAGTTAT+TGG | 0.246021 | 7.4:+84600990 | MS.gene91584:CDS |
| GAAACCATTTCAACTTTCTT+TGG | 0.269632 | 7.4:-84601207 | None:intergenic |
| TTACCTTCTTTGACATACTA+TGG | 0.279036 | 7.4:+84600059 | MS.gene91584:CDS |
| GTACTATAAACCTCAAATTT+AGG | 0.281913 | 7.4:-84601165 | None:intergenic |
| ACATGATCAAGCTTCTGTTT+TGG | 0.289012 | 7.4:+84600384 | MS.gene91584:CDS |
| CCAATTGAAAATCCATAGTT+TGG | 0.297340 | 7.4:-84600427 | None:intergenic |
| AACCAACAATAGAAGCTTAA+TGG | 0.299594 | 7.4:+84600666 | MS.gene91584:CDS |
| GTTATTTCAATTATCTCGTT+TGG | 0.307673 | 7.4:+84600726 | MS.gene91584:CDS |
| GTTGAAACAAGTGAGTTATT+GGG | 0.310202 | 7.4:+84600991 | MS.gene91584:CDS |
| ACGCAAAATATTTGAGTGAT+TGG | 0.311894 | 7.4:+84600629 | MS.gene91584:CDS |
| ACAATAGAAGCTTAATGGTT+TGG | 0.327026 | 7.4:+84600671 | MS.gene91584:CDS |
| GTTCCAGAAGATTCATTTAG+AGG | 0.333102 | 7.4:+84600703 | MS.gene91584:CDS |
| AACCATTAAGCTTCTATTGT+TGG | 0.335300 | 7.4:-84600668 | None:intergenic |
| TTTGGATATTGAAAAGCTTA+AGG | 0.336799 | 7.4:+84600744 | MS.gene91584:CDS |
| CAATAGAAGCTTAATGGTTT+GGG | 0.346870 | 7.4:+84600672 | MS.gene91584:CDS |
| TGTCTTTCTGATTGTAGAAA+TGG | 0.347109 | 7.4:+84601252 | MS.gene91584:CDS |
| GAGGTTTATAGTACTGATTT+TGG | 0.352559 | 7.4:+84601174 | MS.gene91584:CDS |
| AACCCTCTAAATGAATCTTC+TGG | 0.355233 | 7.4:-84600706 | None:intergenic |
| TTGTATCCATAGCTTATGCC+TGG | 0.358494 | 7.4:+84600833 | MS.gene91584:CDS |
| TAGAAGAAAATTCTTTGAAC+AGG | 0.373178 | 7.4:-84600094 | None:intergenic |
| TGCTGGTTCACCTAAATTTG+AGG | 0.384622 | 7.4:+84601155 | MS.gene91584:CDS |
| TGAAGTGTTAAGTGAAGCTT+TGG | 0.391195 | 7.4:+84601038 | MS.gene91584:CDS |
| ATTTCTGATGTTAAAGCAAT+TGG | 0.393974 | 7.4:+84601129 | MS.gene91584:CDS |
| TCATGTGAAATATTCAAGTT+AGG | 0.394263 | 7.4:-84600367 | None:intergenic |
| ATTTGTAGTGTCAAAGCAAA+AGG | 0.403561 | 7.4:+84600768 | MS.gene91584:CDS |
| CAATTTCCAAAATATGTTGC+AGG | 0.404618 | 7.4:-84600946 | None:intergenic |
| CCTTTGATGAATTTAATGAT+TGG | 0.405006 | 7.4:-84600247 | None:intergenic |
| TTAAAAGATGGAGTGTTGAA+TGG | 0.421534 | 7.4:+84601066 | MS.gene91584:CDS |
| AAAACCAAAGAAAGTTGAAA+TGG | 0.434697 | 7.4:+84601203 | MS.gene91584:CDS |
| TTGCTTCGCTCTTTGTCAAA+GGG | 0.440215 | 7.4:+84601325 | MS.gene91584:CDS |
| AATGGTGATGGTGTTGAGAT+AGG | 0.443549 | 7.4:+84601270 | MS.gene91584:CDS |
| GCTTTGGAAACTTTAAAAGA+TGG | 0.454178 | 7.4:+84601054 | MS.gene91584:CDS |
| ATTACATTTCTATCATAGAA+AGG | 0.455442 | 7.4:-84600586 | None:intergenic |
| TTTGCTTCGCTCTTTGTCAA+AGG | 0.460307 | 7.4:+84601324 | MS.gene91584:CDS |
| AAACTCATGTGGCAAACAAA+GGG | 0.475884 | 7.4:-84600560 | None:intergenic |
| TTGAAACAAGTGAGTTATTG+GGG | 0.479171 | 7.4:+84600992 | MS.gene91584:CDS |
| AGTATGTCAAAGAAGGTAAG+AGG | 0.485002 | 7.4:-84600055 | None:intergenic |
| CCAAAATATGTTGCAGGAGT+CGG | 0.485029 | 7.4:-84600940 | None:intergenic |
| ATGGTTTCTATAGATAGAAC+TGG | 0.490163 | 7.4:+84601222 | MS.gene91584:CDS |
| GTTAAAGCAATTGGTGCTGC+TGG | 0.491334 | 7.4:+84601138 | MS.gene91584:CDS |
| TGTCAAAGCAAAAGGGTACT+AGG | 0.499646 | 7.4:+84600776 | MS.gene91584:CDS |
| AAGAAAATTCTTTGAACAGG+TGG | 0.502827 | 7.4:-84600091 | None:intergenic |
| GGTGGTTCTTCTTGTGAAGA+TGG | 0.512780 | 7.4:-84600016 | None:intergenic |
| TAACCATAGTATGTCAAAGA+AGG | 0.512933 | 7.4:-84600062 | None:intergenic |
| TTCCAGAAGATTCATTTAGA+GGG | 0.521713 | 7.4:+84600704 | MS.gene91584:CDS |
| TGGCTTTGAATATTGATTGT+CGG | 0.525216 | 7.4:+84600902 | MS.gene91584:CDS |
| TTGAGTGATTGGTTAAAGCA+AGG | 0.525666 | 7.4:+84600640 | MS.gene91584:CDS |
| CTATGATAGAAATGTAATCA+AGG | 0.525898 | 7.4:+84600591 | MS.gene91584:CDS |
| CAACAAAAGTTGACAAGTGA+AGG | 0.526815 | 7.4:-84600813 | None:intergenic |
| GTTTGGGAAGAAGGGTGTTG+AGG | 0.530313 | 7.4:-84600144 | None:intergenic |
| TCTACAATCAGAAAGACAAA+AGG | 0.534606 | 7.4:-84601247 | None:intergenic |
| AGCTTTCATTCTGCATACCC+AGG | 0.541747 | 7.4:-84600851 | None:intergenic |
| GTATTTGTGTAGCTTCAGAA+AGG | 0.547490 | 7.4:-84600333 | None:intergenic |
| GCATACCCAGGCATAAGCTA+TGG | 0.548203 | 7.4:-84600839 | None:intergenic |
| TGTATCCATAGCTTATGCCT+GGG | 0.550290 | 7.4:+84600834 | MS.gene91584:CDS |
| AAGGGTGTTGAGGAAGAGTG+AGG | 0.553924 | 7.4:-84600134 | None:intergenic |
| GGAAGTGATGTTTCTGAAGG+TGG | 0.560578 | 7.4:-84600034 | None:intergenic |
| CCAATCATTAAATTCATCAA+AGG | 0.568697 | 7.4:+84600247 | MS.gene91584:CDS |
| AAAACTCATGTGGCAAACAA+AGG | 0.574819 | 7.4:-84600561 | None:intergenic |
| TTTGTAGTGTCAAAGCAAAA+GGG | 0.581463 | 7.4:+84600769 | MS.gene91584:CDS |
| CAAATGAGATGACCCAAAAG+AGG | 0.586463 | 7.4:-84600205 | None:intergenic |
| AGGAAGAGTGAGGCTTGGTG+TGG | 0.588661 | 7.4:-84600124 | None:intergenic |
| TGTTGAGGAAGAGTGAGGCT+TGG | 0.589306 | 7.4:-84600129 | None:intergenic |
| ATCAGCATCAGATTCAGCTA+TGG | 0.602424 | 7.4:-84600287 | None:intergenic |
| TCTGATTGTAGAAATGGTGA+TGG | 0.615354 | 7.4:+84601258 | MS.gene91584:CDS |
| AGAGGAAGTGATGTTTCTGA+AGG | 0.617893 | 7.4:-84600037 | None:intergenic |
| AAATATGTTGCAGGAGTCGG+TGG | 0.620986 | 7.4:-84600937 | None:intergenic |
| AGTGATTGGTTAAAGCAAGG+TGG | 0.623091 | 7.4:+84600643 | MS.gene91584:CDS |
| GGAAGAGTGAGGCTTGGTGT+GGG | 0.646190 | 7.4:-84600123 | None:intergenic |
| TAGAAAGGACAAAACTCATG+TGG | 0.652604 | 7.4:-84600571 | None:intergenic |
| AACTCATGTGGCAAACAAAG+GGG | 0.672677 | 7.4:-84600559 | None:intergenic |
| TGAAACAAGTGAGTTATTGG+GGG | 0.676029 | 7.4:+84600993 | MS.gene91584:CDS |
| ATTGGCTTGTGAGAATCATG+TGG | 0.746036 | 7.4:-84600229 | None:intergenic |
| GGAAGATGGACTAATCGTGG+CGG | 0.752782 | 7.4:+84601014 | MS.gene91584:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGAAAATTTCTATGATTT+TGG | - | chr7.4:84599990-84600009 | None:intergenic | 15.0% |
| !! | ATTACATTTCTATCATAGAA+AGG | - | chr7.4:84600589-84600608 | None:intergenic | 20.0% |
| !!! | ACATATTTTGGAAATTGTAT+TGG | + | chr7.4:84600952-84600971 | MS.gene91584:CDS | 20.0% |
| !!! | GAAAGTGATTTTTTTAGTTT+GGG | - | chr7.4:84600163-84600182 | None:intergenic | 20.0% |
| ! | AAAACCAAAGAAAGTTGAAA+TGG | + | chr7.4:84601203-84601222 | MS.gene91584:CDS | 25.0% |
| ! | AAATCCATAGTTTGGAAAAA+CGG | - | chr7.4:84600422-84600441 | None:intergenic | 25.0% |
| ! | ATTTCTGATGTTAAAGCAAT+TGG | + | chr7.4:84601129-84601148 | MS.gene91584:CDS | 25.0% |
| ! | CCAATCATTAAATTCATCAA+AGG | + | chr7.4:84600247-84600266 | MS.gene91584:CDS | 25.0% |
| ! | CCTTTGATGAATTTAATGAT+TGG | - | chr7.4:84600250-84600269 | None:intergenic | 25.0% |
| ! | CTATGATAGAAATGTAATCA+AGG | + | chr7.4:84600591-84600610 | MS.gene91584:CDS | 25.0% |
| ! | GTACTATAAACCTCAAATTT+AGG | - | chr7.4:84601168-84601187 | None:intergenic | 25.0% |
| ! | TAGAAGAAAATTCTTTGAAC+AGG | - | chr7.4:84600097-84600116 | None:intergenic | 25.0% |
| ! | TTTGGATATTGAAAAGCTTA+AGG | + | chr7.4:84600744-84600763 | MS.gene91584:CDS | 25.0% |
| !! | AATTTTTGTTCATCGTTACT+TGG | + | chr7.4:84601096-84601115 | MS.gene91584:CDS | 25.0% |
| !! | ATAAAAATGAGAAAGTGCAA+TGG | - | chr7.4:84600185-84600204 | None:intergenic | 25.0% |
| !! | GACTTCTTTTATCAAATCTT+GGG | + | chr7.4:84600489-84600508 | MS.gene91584:CDS | 25.0% |
| !! | GTTATTTCAATTATCTCGTT+TGG | + | chr7.4:84600726-84600745 | MS.gene91584:CDS | 25.0% |
| !! | TATTTTGCGTCTAATTCATT+TGG | - | chr7.4:84600619-84600638 | None:intergenic | 25.0% |
| !! | TCATGTGAAATATTCAAGTT+AGG | - | chr7.4:84600370-84600389 | None:intergenic | 25.0% |
| !!! | ATTTTTTTAGTTTGGGAAGA+AGG | - | chr7.4:84600156-84600175 | None:intergenic | 25.0% |
| !!! | GGAAAGTGATTTTTTTAGTT+TGG | - | chr7.4:84600164-84600183 | None:intergenic | 25.0% |
| !!! | TATTTTGGAAATTGTATTGG+AGG | + | chr7.4:84600955-84600974 | MS.gene91584:CDS | 25.0% |
| !!! | TCTCATTTTTATCCTCTTTT+GGG | + | chr7.4:84600193-84600212 | MS.gene91584:CDS | 25.0% |
| !!! | TTCTCATTTTTATCCTCTTT+TGG | + | chr7.4:84600192-84600211 | MS.gene91584:CDS | 25.0% |
| !!! | TTTTTTTAGTTTGGGAAGAA+GGG | - | chr7.4:84600155-84600174 | None:intergenic | 25.0% |
| AACCAACAATAGAAGCTTAA+TGG | + | chr7.4:84600666-84600685 | MS.gene91584:CDS | 30.0% | |
| AACCATTAAGCTTCTATTGT+TGG | - | chr7.4:84600671-84600690 | None:intergenic | 30.0% | |
| AAGAAAATTCTTTGAACAGG+TGG | - | chr7.4:84600094-84600113 | None:intergenic | 30.0% | |
| ACAATAGAAGCTTAATGGTT+TGG | + | chr7.4:84600671-84600690 | MS.gene91584:CDS | 30.0% | |
| ACGCAAAATATTTGAGTGAT+TGG | + | chr7.4:84600629-84600648 | MS.gene91584:CDS | 30.0% | |
| ATGGTTTCTATAGATAGAAC+TGG | + | chr7.4:84601222-84601241 | MS.gene91584:CDS | 30.0% | |
| ATTTGTAGTGTCAAAGCAAA+AGG | + | chr7.4:84600768-84600787 | MS.gene91584:CDS | 30.0% | |
| CAATAGAAGCTTAATGGTTT+GGG | + | chr7.4:84600672-84600691 | MS.gene91584:CDS | 30.0% | |
| CAATTTCCAAAATATGTTGC+AGG | - | chr7.4:84600949-84600968 | None:intergenic | 30.0% | |
| CCAATTGAAAATCCATAGTT+TGG | - | chr7.4:84600430-84600449 | None:intergenic | 30.0% | |
| GAAACCATTTCAACTTTCTT+TGG | - | chr7.4:84601210-84601229 | None:intergenic | 30.0% | |
| GTTGAAACAAGTGAGTTATT+GGG | + | chr7.4:84600991-84601010 | MS.gene91584:CDS | 30.0% | |
| TAACCATAGTATGTCAAAGA+AGG | - | chr7.4:84600065-84600084 | None:intergenic | 30.0% | |
| TAAGAACAAAAATGCTCTGA+TGG | + | chr7.4:84600882-84600901 | MS.gene91584:CDS | 30.0% | |
| TCTACAATCAGAAAGACAAA+AGG | - | chr7.4:84601250-84601269 | None:intergenic | 30.0% | |
| TGTCTTTCTGATTGTAGAAA+TGG | + | chr7.4:84601252-84601271 | MS.gene91584:CDS | 30.0% | |
| TGTGTCTAACAAAAAAGCAA+TGG | + | chr7.4:84601296-84601315 | MS.gene91584:CDS | 30.0% | |
| TGTTGAAACAAGTGAGTTAT+TGG | + | chr7.4:84600990-84601009 | MS.gene91584:CDS | 30.0% | |
| TTACCTTCTTTGACATACTA+TGG | + | chr7.4:84600059-84600078 | MS.gene91584:CDS | 30.0% | |
| TTCCAGAAGATTCATTTAGA+GGG | + | chr7.4:84600704-84600723 | MS.gene91584:CDS | 30.0% | |
| TTGAAACAAGTGAGTTATTG+GGG | + | chr7.4:84600992-84601011 | MS.gene91584:CDS | 30.0% | |
| TTTGTAGTGTCAAAGCAAAA+GGG | + | chr7.4:84600769-84600788 | MS.gene91584:CDS | 30.0% | |
| ! | CCAAACTATGGATTTTCAAT+TGG | + | chr7.4:84600427-84600446 | MS.gene91584:CDS | 30.0% |
| ! | CGACTTCTTTTATCAAATCT+TGG | + | chr7.4:84600488-84600507 | MS.gene91584:CDS | 30.0% |
| !! | GCTTTGGAAACTTTAAAAGA+TGG | + | chr7.4:84601054-84601073 | MS.gene91584:CDS | 30.0% |
| !! | TGGCTTTGAATATTGATTGT+CGG | + | chr7.4:84600902-84600921 | MS.gene91584:CDS | 30.0% |
| !! | TTAAAAGATGGAGTGTTGAA+TGG | + | chr7.4:84601066-84601085 | MS.gene91584:CDS | 30.0% |
| !!! | GAGGTTTATAGTACTGATTT+TGG | + | chr7.4:84601174-84601193 | MS.gene91584:CDS | 30.0% |
| !!! | TATAGTACTGATTTTGGTTG+TGG | + | chr7.4:84601180-84601199 | MS.gene91584:CDS | 30.0% |
| AAAACTCATGTGGCAAACAA+AGG | - | chr7.4:84600564-84600583 | None:intergenic | 35.0% | |
| AAACTCATGTGGCAAACAAA+GGG | - | chr7.4:84600563-84600582 | None:intergenic | 35.0% | |
| AACCCTCTAAATGAATCTTC+TGG | - | chr7.4:84600709-84600728 | None:intergenic | 35.0% | |
| AGTATGTCAAAGAAGGTAAG+AGG | - | chr7.4:84600058-84600077 | None:intergenic | 35.0% | |
| CAACAAAAGTTGACAAGTGA+AGG | - | chr7.4:84600816-84600835 | None:intergenic | 35.0% | |
| GTATTTGTGTAGCTTCAGAA+AGG | - | chr7.4:84600336-84600355 | None:intergenic | 35.0% | |
| GTTCCAGAAGATTCATTTAG+AGG | + | chr7.4:84600703-84600722 | MS.gene91584:CDS | 35.0% | |
| TAGAAAGGACAAAACTCATG+TGG | - | chr7.4:84600574-84600593 | None:intergenic | 35.0% | |
| TCTGATTGTAGAAATGGTGA+TGG | + | chr7.4:84601258-84601277 | MS.gene91584:CDS | 35.0% | |
| TGAAACAAGTGAGTTATTGG+GGG | + | chr7.4:84600993-84601012 | MS.gene91584:CDS | 35.0% | |
| ! | ACATGATCAAGCTTCTGTTT+TGG | + | chr7.4:84600384-84600403 | MS.gene91584:CDS | 35.0% |
| ! | GCTGATTTCAATCATCTTTC+AGG | + | chr7.4:84600304-84600323 | MS.gene91584:CDS | 35.0% |
| ! | TGAAGTGTTAAGTGAAGCTT+TGG | + | chr7.4:84601038-84601057 | MS.gene91584:CDS | 35.0% |
| ! | TTGAGTGATTGGTTAAAGCA+AGG | + | chr7.4:84600640-84600659 | MS.gene91584:CDS | 35.0% |
| ! | TTTTGTTCATCGTTACTTGG+CGG | + | chr7.4:84601099-84601118 | MS.gene91584:CDS | 35.0% |
| !! | GTTACCGTTTTTCCAAACTA+TGG | + | chr7.4:84600415-84600434 | MS.gene91584:CDS | 35.0% |
| !!! | TCACATCATGCTGTTTTAGA+TGG | + | chr7.4:84600457-84600476 | MS.gene91584:CDS | 35.0% |
| AACTCATGTGGCAAACAAAG+GGG | - | chr7.4:84600562-84600581 | None:intergenic | 40.0% | |
| ATCAGCATCAGATTCAGCTA+TGG | - | chr7.4:84600290-84600309 | None:intergenic | 40.0% | |
| CAAATGAGATGACCCAAAAG+AGG | - | chr7.4:84600208-84600227 | None:intergenic | 40.0% | |
| CCAAAATATGTTGCAGGAGT+CGG | - | chr7.4:84600943-84600962 | None:intergenic | 40.0% | |
| TGTATCCATAGCTTATGCCT+GGG | + | chr7.4:84600834-84600853 | MS.gene91584:CDS | 40.0% | |
| TTGCTTCGCTCTTTGTCAAA+GGG | + | chr7.4:84601325-84601344 | MS.gene91584:CDS | 40.0% | |
| TTGTATCCATAGCTTATGCC+TGG | + | chr7.4:84600833-84600852 | MS.gene91584:CDS | 40.0% | |
| TTTGCTTCGCTCTTTGTCAA+AGG | + | chr7.4:84601324-84601343 | MS.gene91584:CDS | 40.0% | |
| ! | AGAGGAAGTGATGTTTCTGA+AGG | - | chr7.4:84600040-84600059 | None:intergenic | 40.0% |
| ! | AGTGATTGGTTAAAGCAAGG+TGG | + | chr7.4:84600643-84600662 | MS.gene91584:CDS | 40.0% |
| !! | AATGGTGATGGTGTTGAGAT+AGG | + | chr7.4:84601270-84601289 | MS.gene91584:CDS | 40.0% |
| !! | ATTGGCTTGTGAGAATCATG+TGG | - | chr7.4:84600232-84600251 | None:intergenic | 40.0% |
| !! | CTCTTTTGGGTCATCTCATT+TGG | + | chr7.4:84600206-84600225 | MS.gene91584:CDS | 40.0% |
| !! | TGCTGGTTCACCTAAATTTG+AGG | + | chr7.4:84601155-84601174 | MS.gene91584:CDS | 40.0% |
| !! | TGTCAAAGCAAAAGGGTACT+AGG | + | chr7.4:84600776-84600795 | MS.gene91584:CDS | 40.0% |
| AAATATGTTGCAGGAGTCGG+TGG | - | chr7.4:84600940-84600959 | None:intergenic | 45.0% | |
| AGCTTTCATTCTGCATACCC+AGG | - | chr7.4:84600854-84600873 | None:intergenic | 45.0% | |
| AGTGAGTTATTGGGGGAAGA+TGG | + | chr7.4:84601000-84601019 | MS.gene91584:CDS | 45.0% | |
| GGTGGTTCTTCTTGTGAAGA+TGG | - | chr7.4:84600019-84600038 | None:intergenic | 45.0% | |
| ! | CCGACTCCTGCAACATATTT+TGG | + | chr7.4:84600940-84600959 | MS.gene91584:CDS | 45.0% |
| ! | GGAAGTGATGTTTCTGAAGG+TGG | - | chr7.4:84600037-84600056 | None:intergenic | 45.0% |
| !! | GTTAAAGCAATTGGTGCTGC+TGG | + | chr7.4:84601138-84601157 | MS.gene91584:CDS | 45.0% |
| GCATACCCAGGCATAAGCTA+TGG | - | chr7.4:84600842-84600861 | None:intergenic | 50.0% | |
| TGTTGAGGAAGAGTGAGGCT+TGG | - | chr7.4:84600132-84600151 | None:intergenic | 50.0% | |
| !! | AAGGGTGTTGAGGAAGAGTG+AGG | - | chr7.4:84600137-84600156 | None:intergenic | 50.0% |
| !! | GGAAGATGGACTAATCGTGG+CGG | + | chr7.4:84601014-84601033 | MS.gene91584:CDS | 50.0% |
| !! | GTTTGGGAAGAAGGGTGTTG+AGG | - | chr7.4:84600147-84600166 | None:intergenic | 50.0% |
| !! | AGGAAGAGTGAGGCTTGGTG+TGG | - | chr7.4:84600127-84600146 | None:intergenic | 55.0% |
| !! | GGAAGAGTGAGGCTTGGTGT+GGG | - | chr7.4:84600126-84600145 | None:intergenic | 55.0% |
| !! | GGGGGAAGATGGACTAATCG+TGG | + | chr7.4:84601011-84601030 | MS.gene91584:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 84599983 | 84601359 | 84599983 | ID=MS.gene91584 |
| chr7.4 | mRNA | 84599983 | 84601359 | 84599983 | ID=MS.gene91584.t1;Parent=MS.gene91584 |
| chr7.4 | exon | 84599983 | 84601359 | 84599983 | ID=MS.gene91584.t1.exon1;Parent=MS.gene91584.t1 |
| chr7.4 | CDS | 84599983 | 84601359 | 84599983 | ID=cds.MS.gene91584.t1;Parent=MS.gene91584.t1 |
| Gene Sequence |
| Protein sequence |
