Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34376.t1 | RHN81973.1 | 94.7 | 75 | 4 | 0 | 1 | 75 | 1 | 75 | 2.80E-32 | 147.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34376.t1 | B7FF93 | 94.7 | 75 | 4 | 0 | 1 | 75 | 1 | 75 | 2.0e-32 | 147.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene34376 | 0.877505 | 5.96E-69 | -1.69E-46 |
| MS.gene049369 | MS.gene34376 | 0.866034 | 3.83E-65 | -1.69E-46 |
| MS.gene049396 | MS.gene34376 | 0.836642 | 8.30E-57 | -1.69E-46 |
| MS.gene050122 | MS.gene34376 | 0.824405 | 8.22E-54 | -1.69E-46 |
| MS.gene050128 | MS.gene34376 | 0.810234 | 1.28E-50 | -1.69E-46 |
| MS.gene050215 | MS.gene34376 | 0.800383 | 1.48E-48 | -1.69E-46 |
| MS.gene050432 | MS.gene34376 | 0.80088 | 1.17E-48 | -1.69E-46 |
| MS.gene050670 | MS.gene34376 | 0.844655 | 6.61E-59 | -1.69E-46 |
| MS.gene050971 | MS.gene34376 | 0.819654 | 1.04E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene34376 | 0.837818 | 4.15E-57 | -1.69E-46 |
| MS.gene051360 | MS.gene34376 | 0.815683 | 8.15E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene34376 | 0.801089 | 1.06E-48 | -1.69E-46 |
| MS.gene052077 | MS.gene34376 | 0.810711 | 1.01E-50 | -1.69E-46 |
| MS.gene052136 | MS.gene34376 | 0.815875 | 7.39E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene34376 | 0.833418 | 5.40E-56 | -1.69E-46 |
| MS.gene052253 | MS.gene34376 | 0.815894 | 7.32E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene34376 | 0.817486 | 3.22E-52 | -1.69E-46 |
| MS.gene052414 | MS.gene34376 | 0.845778 | 3.29E-59 | -1.69E-46 |
| MS.gene052485 | MS.gene34376 | 0.829556 | 4.82E-55 | -1.69E-46 |
| MS.gene05316 | MS.gene34376 | 0.82319 | 1.58E-53 | -1.69E-46 |
| MS.gene053209 | MS.gene34376 | 0.800051 | 1.73E-48 | -1.69E-46 |
| MS.gene053449 | MS.gene34376 | 0.856337 | 3.44E-62 | -1.69E-46 |
| MS.gene053813 | MS.gene34376 | 0.813247 | 2.82E-51 | -1.69E-46 |
| MS.gene053912 | MS.gene34376 | 0.824845 | 6.47E-54 | -1.69E-46 |
| MS.gene054349 | MS.gene34376 | 0.818865 | 1.57E-52 | -1.69E-46 |
| MS.gene05505 | MS.gene34376 | 0.808597 | 2.86E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene34376 | 0.867996 | 9.06E-66 | -1.69E-46 |
| MS.gene055909 | MS.gene34376 | 0.814912 | 1.21E-51 | -1.69E-46 |
| MS.gene055911 | MS.gene34376 | 0.816565 | 5.18E-52 | -1.69E-46 |
| MS.gene05592 | MS.gene34376 | 0.800509 | 1.39E-48 | -1.69E-46 |
| MS.gene056191 | MS.gene34376 | 0.800016 | 1.76E-48 | -1.69E-46 |
| MS.gene056407 | MS.gene34376 | 0.811578 | 6.53E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene34376 | 0.864088 | 1.56E-64 | -1.69E-46 |
| MS.gene056511 | MS.gene34376 | 0.877116 | 8.13E-69 | -1.69E-46 |
| MS.gene05664 | MS.gene34376 | 0.804423 | 2.18E-49 | -1.69E-46 |
| MS.gene05735 | MS.gene34376 | 0.801305 | 9.59E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene34376 | 0.816841 | 4.49E-52 | -1.69E-46 |
| MS.gene057448 | MS.gene34376 | 0.841487 | 4.61E-58 | -1.69E-46 |
| MS.gene057449 | MS.gene34376 | 0.830454 | 2.91E-55 | -1.69E-46 |
| MS.gene057450 | MS.gene34376 | 0.836495 | 9.05E-57 | -1.69E-46 |
| MS.gene057537 | MS.gene34376 | 0.819196 | 1.32E-52 | -1.69E-46 |
| MS.gene057734 | MS.gene34376 | 0.834214 | 3.41E-56 | -1.69E-46 |
| MS.gene057749 | MS.gene34376 | 0.816906 | 4.34E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene34376 | 0.819838 | 9.41E-53 | -1.69E-46 |
| MS.gene057927 | MS.gene34376 | 0.835656 | 1.48E-56 | -1.69E-46 |
| MS.gene058007 | MS.gene34376 | 0.805511 | 1.29E-49 | -1.69E-46 |
| MS.gene058075 | MS.gene34376 | 0.827672 | 1.37E-54 | -1.69E-46 |
| MS.gene058459 | MS.gene34376 | 0.825161 | 5.45E-54 | -1.69E-46 |
| MS.gene05858 | MS.gene34376 | 0.801171 | 1.02E-48 | -1.69E-46 |
| MS.gene058790 | MS.gene34376 | 0.802007 | 6.88E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene34376 | 0.854255 | 1.39E-61 | -1.69E-46 |
| MS.gene058949 | MS.gene34376 | 0.808396 | 3.16E-50 | -1.69E-46 |
| MS.gene058995 | MS.gene34376 | 0.810064 | 1.39E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene34376 | 0.807134 | 5.86E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene34376 | 0.823124 | 1.64E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene34376 | 0.811259 | 7.66E-51 | -1.69E-46 |
| MS.gene059366 | MS.gene34376 | 0.879593 | 1.10E-69 | -1.69E-46 |
| MS.gene059368 | MS.gene34376 | 0.825841 | 3.76E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene34376 | 0.838676 | 2.50E-57 | -1.69E-46 |
| MS.gene059674 | MS.gene34376 | 0.838131 | 3.45E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene34376 | 0.841049 | 6.01E-58 | -1.69E-46 |
| MS.gene059692 | MS.gene34376 | 0.822961 | 1.79E-53 | -1.69E-46 |
| MS.gene059695 | MS.gene34376 | 0.823983 | 1.03E-53 | -1.69E-46 |
| MS.gene059711 | MS.gene34376 | 0.837606 | 4.71E-57 | -1.69E-46 |
| MS.gene059807 | MS.gene34376 | 0.909856 | 3.58E-82 | -1.69E-46 |
| MS.gene059876 | MS.gene34376 | 0.834126 | 3.59E-56 | -1.69E-46 |
| MS.gene059933 | MS.gene34376 | 0.819144 | 1.35E-52 | -1.69E-46 |
| MS.gene060247 | MS.gene34376 | 0.803727 | 3.04E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene34376 | 0.81573 | 7.96E-52 | -1.69E-46 |
| MS.gene060403 | MS.gene34376 | 0.828838 | 7.20E-55 | -1.69E-46 |
| MS.gene060495 | MS.gene34376 | 0.841421 | 4.80E-58 | -1.69E-46 |
| MS.gene060804 | MS.gene34376 | 0.818165 | 2.26E-52 | -1.69E-46 |
| MS.gene060835 | MS.gene34376 | 0.80128 | 9.70E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene34376 | 0.856264 | 3.62E-62 | -1.69E-46 |
| MS.gene061041 | MS.gene34376 | 0.829303 | 5.55E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene34376 | 0.823001 | 1.75E-53 | -1.69E-46 |
| MS.gene061051 | MS.gene34376 | -0.843146 | 1.68E-58 | -1.69E-46 |
| MS.gene061154 | MS.gene34376 | 0.831864 | 1.31E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34376.t1 | MTR_1g103050 | 97.260 | 73 | 2 | 0 | 1 | 73 | 1 | 73 | 2.13e-48 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATAAATTCTAGCTAGTT+AGG | 0.275360 | 1.2:+76717080 | None:intergenic |
| GTGCATCCAATGATTCCTTT+AGG | 0.352281 | 1.2:+76717275 | None:intergenic |
| CTCTTACCTAAAGGAATCAT+TGG | 0.362848 | 1.2:-76717281 | MS.gene34376:CDS |
| TTTGAAAGCAGTTTCATCTA+AGG | 0.366099 | 1.2:+76717231 | None:intergenic |
| TTTCTTCTGTAGGCGTTGTT+TGG | 0.381475 | 1.2:+76717207 | None:intergenic |
| CAACGCCTACAGAAGAAACT+TGG | 0.500663 | 1.2:-76717202 | MS.gene34376:CDS |
| GCTGGTAGGCTACCTGGTGT+TGG | 0.511550 | 1.2:-76717370 | MS.gene34376:CDS |
| TGAATGGCTGGTAGGCTACC+TGG | 0.514647 | 1.2:-76717376 | MS.gene34376:CDS |
| GGATTTGATGTACCTGGAAG+AGG | 0.518796 | 1.2:+76717168 | None:intergenic |
| GGTAAAGAAGAGTGAATGGC+TGG | 0.520572 | 1.2:-76717388 | None:intergenic |
| CTACAGAGACTCTTACCTAA+AGG | 0.529350 | 1.2:-76717290 | MS.gene34376:CDS |
| AGTGACCAAGTTTCTTCTGT+AGG | 0.561380 | 1.2:+76717197 | None:intergenic |
| CTTGGTGACAATCCAACACC+AGG | 0.576332 | 1.2:+76717358 | None:intergenic |
| GTAGTAGTCATTGAAGTCCA+TGG | 0.592320 | 1.2:+76717253 | None:intergenic |
| AAGAAGAGTGAATGGCTGGT+AGG | 0.606425 | 1.2:-76717384 | None:intergenic |
| CTTTAGGTAAGAGTCTCTGT+AGG | 0.619274 | 1.2:+76717291 | None:intergenic |
| AGGAATCATTGGATGCACCA+TGG | 0.643184 | 1.2:-76717270 | MS.gene34376:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAACTGTATTATTATGT+AGG | + | chr1.2:76717323-76717342 | None:intergenic | 15.0% |
| !!! | TAATTTTCTGATCTTTTTCT+TGG | + | chr1.2:76717130-76717149 | None:intergenic | 20.0% |
| !!! | TTTTTGAAGAAACAAGAAAT+TGG | - | chr1.2:76717347-76717366 | MS.gene34376:CDS | 20.0% |
| TTTGAAAGCAGTTTCATCTA+AGG | + | chr1.2:76717239-76717258 | None:intergenic | 30.0% | |
| CTCTTACCTAAAGGAATCAT+TGG | - | chr1.2:76717186-76717205 | MS.gene34376:CDS | 35.0% | |
| TATGTAGGATTTGATGTACC+TGG | + | chr1.2:76717308-76717327 | None:intergenic | 35.0% | |
| AGTGACCAAGTTTCTTCTGT+AGG | + | chr1.2:76717273-76717292 | None:intergenic | 40.0% | |
| CTACAGAGACTCTTACCTAA+AGG | - | chr1.2:76717177-76717196 | MS.gene34376:intron | 40.0% | |
| CTTTAGGTAAGAGTCTCTGT+AGG | + | chr1.2:76717179-76717198 | None:intergenic | 40.0% | |
| GTAGTAGTCATTGAAGTCCA+TGG | + | chr1.2:76717217-76717236 | None:intergenic | 40.0% | |
| GTGCATCCAATGATTCCTTT+AGG | + | chr1.2:76717195-76717214 | None:intergenic | 40.0% | |
| ! | TTTCTTCTGTAGGCGTTGTT+TGG | + | chr1.2:76717263-76717282 | None:intergenic | 40.0% |
| AGGAATCATTGGATGCACCA+TGG | - | chr1.2:76717197-76717216 | MS.gene34376:CDS | 45.0% | |
| CAACGCCTACAGAAGAAACT+TGG | - | chr1.2:76717265-76717284 | MS.gene34376:CDS | 45.0% | |
| GGATTTGATGTACCTGGAAG+AGG | + | chr1.2:76717302-76717321 | None:intergenic | 45.0% | |
| ! | GTCACTTCTTTTCCTCTTCC+AGG | - | chr1.2:76717287-76717306 | MS.gene34376:CDS | 45.0% |
| ! | CTTGGTGACAATCCAACACC+AGG | + | chr1.2:76717112-76717131 | None:intergenic | 50.0% |
| GCTGGTAGGCTACCTGGTGT+TGG | - | chr1.2:76717097-76717116 | MS.gene34376:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 76717094 | 76717395 | 76717094 | ID=MS.gene34376 |
| chr1.2 | mRNA | 76717094 | 76717395 | 76717094 | ID=MS.gene34376.t1;Parent=MS.gene34376 |
| chr1.2 | exon | 76717181 | 76717395 | 76717181 | ID=MS.gene34376.t1.exon1;Parent=MS.gene34376.t1 |
| chr1.2 | CDS | 76717181 | 76717395 | 76717181 | ID=cds.MS.gene34376.t1;Parent=MS.gene34376.t1 |
| chr1.2 | exon | 76717094 | 76717133 | 76717094 | ID=MS.gene34376.t1.exon2;Parent=MS.gene34376.t1 |
| chr1.2 | CDS | 76717094 | 76717133 | 76717094 | ID=cds.MS.gene34376.t1;Parent=MS.gene34376.t1 |
| Gene Sequence |
| Protein sequence |
