Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05059.t1 | XP_003600586.1 | 100 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 7.50E-31 | 142.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05059.t1 | G7J780 | 100.0 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 5.4e-31 | 142.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05059 | MS.gene052239 | -0.804908 | 1.72E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene061796 | 0.807398 | 5.16E-50 | -1.69E-46 |
| MS.gene05059 | MS.gene065069 | 0.804182 | 2.44E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene066513 | 0.802074 | 6.67E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene068028 | 0.803647 | 3.15E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene42653 | 0.806303 | 8.78E-50 | -1.69E-46 |
| MS.gene05059 | MS.gene45281 | 0.804395 | 2.20E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene57360 | 0.801769 | 7.70E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene72748 | 0.803419 | 3.52E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene80201 | 0.80251 | 5.42E-49 | -1.69E-46 |
| MS.gene05059 | MS.gene87403 | 0.803843 | 2.87E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05059.t1 | MTR_3g063970 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 4.56e-47 | 144 |
| MS.gene05059.t1 | MTR_3g063970 | 100.000 | 48 | 0 | 0 | 1 | 48 | 1 | 48 | 8.21e-17 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05059.t1 | AT3G14430 | 63.492 | 63 | 22 | 1 | 9 | 71 | 15 | 76 | 3.06e-24 | 86.7 |
Find 15 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAACATCAATTCCTTTAAA+AGG | 0.168257 | 3.4:-65815810 | None:intergenic |
| ATTTCTCACAAAGGCGAAAA+TGG | 0.321389 | 3.4:-65814792 | None:intergenic |
| ACAAATACTTGATTGTATTC+AGG | 0.336136 | 3.4:-65815755 | None:intergenic |
| CTGGTGGTGGACCAGAGCTT+TGG | 0.389186 | 3.4:+65815786 | MS.gene05059:CDS |
| CTTTAAAAGGGCCAAAGCTC+TGG | 0.402112 | 3.4:-65815797 | None:intergenic |
| AAACATCAATTCCTTTAAAA+GGG | 0.443719 | 3.4:-65815809 | None:intergenic |
| CAAAGCTCTGGTCCACCACC+AGG | 0.488950 | 3.4:-65815785 | None:intergenic |
| CAAGTATTTGTGAAGAAGCC+TGG | 0.508253 | 3.4:+65815767 | MS.gene05059:CDS |
| TGAAGAAGAAAAGAAAGAAG+AGG | 0.565354 | 3.4:-65814829 | None:intergenic |
| GAGAGAAAACAACGATGGCT+AGG | 0.568009 | 3.4:+65814720 | None:intergenic |
| TTTGTGAAGAAGCCTGGTGG+TGG | 0.604245 | 3.4:+65815773 | MS.gene05059:CDS |
| AGAACGAGTCGTTATTGACT+CGG | 0.608689 | 3.4:+65814759 | MS.gene05059:CDS |
| AAACTCAGCATTTCTCACAA+AGG | 0.666973 | 3.4:-65814801 | None:intergenic |
| AGAAAACAACGATGGCTAGG+CGG | 0.694012 | 3.4:+65814723 | None:intergenic |
| GTATTTGTGAAGAAGCCTGG+TGG | 0.787083 | 3.4:+65815770 | MS.gene05059:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGGATAATTATATCAAAA+AGG | + | chr3.4:65815064-65815083 | MS.gene05059:intron | 15.0% |
| !! | AGATTCACAAATTTATATGT+CGG | + | chr3.4:65815300-65815319 | MS.gene05059:intron | 20.0% |
| !! | ATAATTCATTCATATATGGT+TGG | + | chr3.4:65815588-65815607 | MS.gene05059:intron | 20.0% |
| !! | CTTCATAATTCATTCATATA+TGG | + | chr3.4:65815584-65815603 | MS.gene05059:intron | 20.0% |
| !! | TTAGTTACTAAATTGAAAAC+TGG | + | chr3.4:65815030-65815049 | MS.gene05059:intron | 20.0% |
| !!! | CTTTTAATGTTTGAGAATTT+AGG | + | chr3.4:65814980-65814999 | MS.gene05059:intron | 20.0% |
| !!! | TTTTGATAAGTGGAAATTTT+TGG | + | chr3.4:65815611-65815630 | MS.gene05059:intron | 20.0% |
| ! | AACTATATACCAATTCCAAT+TGG | - | chr3.4:65815104-65815123 | None:intergenic | 25.0% |
| ! | ACAAATACTTGATTGTATTC+AGG | - | chr3.4:65815758-65815777 | None:intergenic | 25.0% |
| ! | ATTTGAACAAATGATGCAAA+TGG | - | chr3.4:65814927-65814946 | None:intergenic | 25.0% |
| ! | TATGAATGAATTATGAAGCA+CGG | - | chr3.4:65815582-65815601 | None:intergenic | 25.0% |
| ! | TTAAATTACAATCAGCATGT+TGG | + | chr3.4:65815367-65815386 | MS.gene05059:intron | 25.0% |
| ! | TTCAATGTAATCATATGTGT+CGG | + | chr3.4:65815492-65815511 | MS.gene05059:intron | 25.0% |
| ! | TTTGAACAAATGATGCAAAT+GGG | - | chr3.4:65814926-65814945 | None:intergenic | 25.0% |
| !! | TTGTCTTGTAATTTCACTTT+TGG | + | chr3.4:65815687-65815706 | MS.gene05059:intron | 25.0% |
| !!! | AATTTTCGTTCTTTTTCATG+TGG | + | chr3.4:65814944-65814963 | MS.gene05059:intron | 25.0% |
| !!! | ATTTTCGTTCTTTTTCATGT+GGG | + | chr3.4:65814945-65814964 | MS.gene05059:intron | 25.0% |
| ATTCTGATTCCAATTGGAAT+TGG | + | chr3.4:65815092-65815111 | MS.gene05059:intron | 30.0% | |
| CATAAAGAGAAAAAACAGAG+AGG | - | chr3.4:65814892-65814911 | None:intergenic | 30.0% | |
| TGAAATTACAAGACAAACAG+AGG | - | chr3.4:65815683-65815702 | None:intergenic | 30.0% | |
| TGAAGAAGAAAAGAAAGAAG+AGG | - | chr3.4:65814832-65814851 | None:intergenic | 30.0% | |
| !! | AAACTGGTTTGATGATTGAT+TGG | + | chr3.4:65815046-65815065 | MS.gene05059:intron | 30.0% |
| !!! | ATATGGTTGGTTTTGATAAG+TGG | + | chr3.4:65815601-65815620 | MS.gene05059:intron | 30.0% |
| AAACTCAGCATTTCTCACAA+AGG | - | chr3.4:65814804-65814823 | None:intergenic | 35.0% | |
| AATTACAAGACAAACAGAGG+AGG | - | chr3.4:65815680-65815699 | None:intergenic | 35.0% | |
| ACTGATGAAAACAACTTACG+AGG | - | chr3.4:65815270-65815289 | None:intergenic | 35.0% | |
| ATTACAAGACAAACAGAGGA+GGG | - | chr3.4:65815679-65815698 | None:intergenic | 35.0% | |
| ATTTCTCACAAAGGCGAAAA+TGG | - | chr3.4:65814795-65814814 | None:intergenic | 35.0% | |
| GATTACGAGTCTGTTTGAAT+TGG | - | chr3.4:65815344-65815363 | None:intergenic | 35.0% | |
| GTAGAGATTCTGATTCCAAT+TGG | + | chr3.4:65815086-65815105 | MS.gene05059:intron | 35.0% | |
| TGGTGTATCATGTATGAACA+CGG | + | chr3.4:65815394-65815413 | MS.gene05059:intron | 35.0% | |
| CAAGTATTTGTGAAGAAGCC+TGG | + | chr3.4:65815767-65815786 | MS.gene05059:CDS | 40.0% | |
| ! | AGAACGAGTCGTTATTGACT+CGG | + | chr3.4:65814759-65814778 | MS.gene05059:CDS | 40.0% |
| !!! | ACAATCAGCATGTTGGCTTT+TGG | + | chr3.4:65815374-65815393 | MS.gene05059:intron | 40.0% |
| ATCATGTATGAACACGGACG+CGG | + | chr3.4:65815400-65815419 | MS.gene05059:intron | 45.0% | |
| CATCAGACACGAAACAGACA+TGG | + | chr3.4:65815430-65815449 | MS.gene05059:intron | 45.0% | |
| GGACACACCTAATCTGAGAA+GGG | + | chr3.4:65815555-65815574 | MS.gene05059:intron | 45.0% | |
| GTATTTGTGAAGAAGCCTGG+TGG | + | chr3.4:65815770-65815789 | MS.gene05059:CDS | 45.0% | |
| TTCTCAGATTAGGTGTGTCC+CGG | - | chr3.4:65815555-65815574 | None:intergenic | 45.0% | |
| !!! | TTATTTTATCAAATTATTAT+TGG | - | chr3.4:65815467-65815486 | None:intergenic | 5.0% |
| AAGACAAACAGAGGAGGGAG+TGG | - | chr3.4:65815674-65815693 | None:intergenic | 50.0% | |
| AGACAAACAGAGGAGGGAGT+GGG | - | chr3.4:65815673-65815692 | None:intergenic | 50.0% | |
| GGGACACACCTAATCTGAGA+AGG | + | chr3.4:65815554-65815573 | MS.gene05059:intron | 50.0% | |
| TTTGTGAAGAAGCCTGGTGG+TGG | + | chr3.4:65815773-65815792 | MS.gene05059:CDS | 50.0% | |
| !! | CATATGTGTCGGTGTCGTGT+TGG | + | chr3.4:65815503-65815522 | MS.gene05059:intron | 50.0% |
| ACAAACAGAGGAGGGAGTGG+GGG | - | chr3.4:65815671-65815690 | None:intergenic | 55.0% | |
| GACAAACAGAGGAGGGAGTG+GGG | - | chr3.4:65815672-65815691 | None:intergenic | 55.0% | |
| GATTAGGTGTGTCCCGGTGT+CGG | - | chr3.4:65815549-65815568 | None:intergenic | 55.0% | |
| GCACGGACCCTTCTCAGATT+AGG | - | chr3.4:65815565-65815584 | None:intergenic | 55.0% | |
| TATGAACACGGACGCGGATG+CGG | + | chr3.4:65815406-65815425 | MS.gene05059:intron | 55.0% | |
| ! | TGTCGGACACGCATTGGTGT+CGG | - | chr3.4:65815532-65815551 | None:intergenic | 55.0% |
| ACCAATGCGTGTCCGACACC+GGG | + | chr3.4:65815534-65815553 | MS.gene05059:intron | 60.0% | |
| CAAACAGAGGAGGGAGTGGG+GGG | - | chr3.4:65815670-65815689 | None:intergenic | 60.0% | |
| CACCAATGCGTGTCCGACAC+CGG | + | chr3.4:65815533-65815552 | MS.gene05059:intron | 60.0% | |
| ! | CAAAGCTCTGGTCCACCACC+AGG | - | chr3.4:65815788-65815807 | None:intergenic | 60.0% |
| !! | CTGGTGGTGGACCAGAGCTT+TGG | + | chr3.4:65815786-65815805 | MS.gene05059:CDS | 60.0% |
| ! | TCCCGGTGTCGGACACGCAT+TGG | - | chr3.4:65815538-65815557 | None:intergenic | 65.0% |
| ACAGAGGAGGGAGTGGGGGG+TGG | - | chr3.4:65815667-65815686 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 65814734 | 65815817 | 65814734 | ID=MS.gene05059 |
| chr3.4 | mRNA | 65814734 | 65815817 | 65814734 | ID=MS.gene05059.t1;Parent=MS.gene05059 |
| chr3.4 | exon | 65814734 | 65814877 | 65814734 | ID=MS.gene05059.t1.exon1;Parent=MS.gene05059.t1 |
| chr3.4 | CDS | 65814734 | 65814877 | 65814734 | ID=cds.MS.gene05059.t1;Parent=MS.gene05059.t1 |
| chr3.4 | exon | 65815746 | 65815817 | 65815746 | ID=MS.gene05059.t1.exon2;Parent=MS.gene05059.t1 |
| chr3.4 | CDS | 65815746 | 65815817 | 65815746 | ID=cds.MS.gene05059.t1;Parent=MS.gene05059.t1 |
| Gene Sequence |
| Protein sequence |
