AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87403


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87403.t1 MTR_8g078100 98.133 375 7 0 1 375 1 375 0.0 756
MS.gene87403.t1 MTR_5g019430 70.292 377 107 2 1 372 1 377 0.0 542
MS.gene87403.t1 MTR_4g069160 58.355 377 151 4 1 375 1 373 3.39e-147 421
MS.gene87403.t1 MTR_1g034480 55.733 375 159 3 1 374 1 369 5.35e-137 395
MS.gene87403.t1 MTR_3g011140 62.162 74 28 0 285 358 1 74 9.19e-26 99.8
MS.gene87403.t1 MTR_8g068520 22.293 314 206 7 76 373 86 377 6.06e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87403.t1 AT2G18630 54.354 379 167 2 1 374 2 379 2.15e-138 399
MS.gene87403.t1 AT2G18630 54.354 379 167 2 1 374 2 379 3.20e-138 399
MS.gene87403.t1 AT4G34320 55.319 376 159 5 1 375 1 368 6.37e-132 382
MS.gene87403.t1 AT4G34320 55.319 376 159 5 1 375 1 368 6.37e-132 382
MS.gene87403.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene87403.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene87403.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene87403.t1 AT5G66675 53.134 367 162 4 16 374 32 396 2.45e-126 370
MS.gene87403.t1 AT4G34330 48.851 348 162 3 24 371 17 348 1.48e-106 317
MS.gene87403.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene87403.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene87403.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene87403.t1 AT5G66670 41.899 358 201 2 21 371 35 392 3.38e-88 272
MS.gene87403.t1 AT5G66660 41.713 362 203 2 21 374 35 396 5.42e-85 263
MS.gene87403.t1 AT3G28270 27.933 358 242 6 28 370 16 372 6.11e-36 135
MS.gene87403.t1 AT3G28270 27.933 358 242 6 28 370 16 372 6.11e-36 135
MS.gene87403.t1 AT3G28290 25.661 378 242 12 24 370 11 380 4.66e-29 116
MS.gene87403.t1 AT3G28300 25.661 378 242 12 24 370 11 380 4.66e-29 116
MS.gene87403.t1 AT3G57100 25.705 319 210 5 65 370 43 347 2.05e-25 105
MS.gene87403.t1 AT3G28320 23.492 315 214 8 80 370 2 313 2.78e-20 90.9

Find 58 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATAAAACTCCTCCGTAAA+AGG 0.159235 8.3:+26085601 None:intergenic
TGAAGTTGTTAAAGTTATTT+TGG 0.163128 8.3:-26085869 MS.gene87403:CDS
TCATCAAAACTTTGCAAGTT+TGG 0.189922 8.3:+26086009 None:intergenic
TCGGGTGCTTACAAACAAAT+TGG 0.255573 8.3:-26085227 MS.gene87403:CDS
TGGATTGCAAGAGGGATATA+TGG 0.257789 8.3:-26085849 MS.gene87403:CDS
TTTGAAGAAGAGGTGCAAAA+TGG 0.287245 8.3:-26085688 MS.gene87403:CDS
TTACATTTCATTGAAGGATT+TGG 0.290195 8.3:-26085257 MS.gene87403:CDS
TTGCTACTGGTGTTGAAGTT+AGG 0.293911 8.3:-26085948 MS.gene87403:CDS
GGGATATGAGAAGGCTATAA+AGG 0.305251 8.3:-26085317 MS.gene87403:CDS
CATTCGGGTCGGTTGGAAAA+TGG 0.337016 8.3:-26085357 MS.gene87403:CDS
GCGGCGTTGGCTGTTCCATT+CGG 0.349853 8.3:-26085373 MS.gene87403:CDS
TGAAGGATTTGGACAACATT+CGG 0.353812 8.3:-26085246 MS.gene87403:CDS
AACAGCTTTGGCTGCGGCGT+TGG 0.383556 8.3:-26085386 MS.gene87403:CDS
GTTATTAGTTCTCTTGCTAC+TGG 0.384834 8.3:-26085961 MS.gene87403:CDS
CGGCGTTGGCTGTTCCATTC+GGG 0.389129 8.3:-26085372 MS.gene87403:CDS
GAAGGATTTGGACAACATTC+GGG 0.389768 8.3:-26085245 MS.gene87403:CDS
GGATTGCAAGAGGGATATAT+GGG 0.412828 8.3:-26085848 MS.gene87403:CDS
TCCGCCCGTTGTAACAGCTT+TGG 0.415627 8.3:-26085398 MS.gene87403:CDS
GAGGTGCAAAATGGGCTTGA+AGG 0.435928 8.3:-26085679 MS.gene87403:CDS
TTGAAGAAGAGGTGCAAAAT+GGG 0.439323 8.3:-26085687 MS.gene87403:CDS
AAGAGAACTAATAACCCTAT+TGG 0.447555 8.3:+26085969 None:intergenic
GGATATGAGAAGGCTATAAA+GGG 0.447749 8.3:-26085316 MS.gene87403:CDS
GTATTAAAGAGCATACCAAT+AGG 0.457807 8.3:-26085984 MS.gene87403:CDS
TGGTACTTACATTTCATTGA+AGG 0.469068 8.3:-26085263 MS.gene87403:CDS
GCAGCCAAAGCTGTTACAAC+GGG 0.472631 8.3:+26085394 None:intergenic
AATTACAAAACTCCAACGTC+TGG 0.474086 8.3:+26085776 None:intergenic
TCTAAGACCTCATCTCAATC+AGG 0.476204 8.3:+26085004 None:intergenic
AGGTGCAAAATGGGCTTGAA+GGG 0.480313 8.3:-26085678 MS.gene87403:CDS
AAACAAGGATTTGTTCGCAT+TGG 0.490218 8.3:-26085824 MS.gene87403:CDS
CTCTTGAGAAATGTTTGAGA+CGG 0.491708 8.3:-26085753 MS.gene87403:CDS
GCTGTTCCATTCGGGTCGGT+TGG 0.492398 8.3:-26085364 MS.gene87403:CDS
TGAAGACATTGCAAGAGCTT+AGG 0.500716 8.3:-26085645 MS.gene87403:CDS
TCAATCGGTTTATACGCAGC+AGG 0.509528 8.3:-26085572 MS.gene87403:CDS
TATTAAAGAGCATACCAATA+GGG 0.511363 8.3:-26085983 MS.gene87403:CDS
CGTTGTAACAGCTTTGGCTG+CGG 0.511718 8.3:-26085392 MS.gene87403:CDS
AATAAAACTCCTCCGTAAAA+GGG 0.512251 8.3:+26085602 None:intergenic
TCTTGAGAAATGTTTGAGAC+GGG 0.517157 8.3:-26085752 MS.gene87403:CDS
CGCAGCCAAAGCTGTTACAA+CGG 0.524710 8.3:+26085393 None:intergenic
ATATGAAGCTGCATGTATCA+AGG 0.527102 8.3:-26086034 MS.gene87403:CDS
AGGGAATTCAAGCAAGATGA+TGG 0.547310 8.3:-26086118 MS.gene87403:CDS
AGTTGAAATCACTCAAGTCA+TGG 0.549628 8.3:-26085501 MS.gene87403:CDS
GAAGGCTATAAAGGGACAAA+GGG 0.553000 8.3:-26085308 MS.gene87403:CDS
AGAAGGCTATAAAGGGACAA+AGG 0.553211 8.3:-26085309 MS.gene87403:CDS
GAATCAAGTGATTGTTAAGT+CGG 0.557993 8.3:-26085722 MS.gene87403:CDS
GCCAAAGCTGTTACAACGGG+CGG 0.569880 8.3:+26085397 None:intergenic
ATCTGAGGTATTAAGATGGG+AGG 0.576566 8.3:-26086138 None:intergenic
GTTGGCTGTTCCATTCGGGT+CGG 0.587471 8.3:-26085368 MS.gene87403:CDS
AATCACTCAAGTCATGGAAG+AGG 0.591019 8.3:-26085495 MS.gene87403:CDS
GATTTGCAATTTCTTCAACA+TGG 0.599579 8.3:+26085546 None:intergenic
TGATAATAGTCTCCAGACGT+TGG 0.605979 8.3:-26085788 MS.gene87403:CDS
GAGGGATATATGGGGAAACA+AGG 0.609168 8.3:-26085839 MS.gene87403:CDS
ATCACTCAAGTCATGGAAGA+GGG 0.610181 8.3:-26085494 MS.gene87403:CDS
GGTGCAAAATGGGCTTGAAG+GGG 0.611042 8.3:-26085677 MS.gene87403:CDS
GTAGTTAGCTCAATGCAGCT+TGG 0.613218 8.3:-26085283 MS.gene87403:CDS
TCTGAGGTATTAAGATGGGA+GGG 0.638076 8.3:-26086137 None:intergenic
GTGCAAAATGGGCTTGAAGG+GGG 0.641754 8.3:-26085676 MS.gene87403:CDS
GATTGCAAGAGGGATATATG+GGG 0.687000 8.3:-26085847 MS.gene87403:CDS
GTCGACAGATAAGAAAAGCG+AGG 0.701787 8.3:-26085057 MS.gene87403:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAATATAGAAACTTTTTC+AGG - chr8.3:26086023-26086042 MS.gene87403:CDS 20.0%
!!! GTTTTATTTGTTGTTTCAAT+CGG - chr8.3:26085554-26085573 MS.gene87403:CDS 20.0%
!!! TGAAGTTGTTAAAGTTATTT+TGG - chr8.3:26085272-26085291 MS.gene87403:CDS 20.0%
! TTACATTTCATTGAAGGATT+TGG - chr8.3:26085884-26085903 MS.gene87403:CDS 25.0%
!! TATTAAAGAGCATACCAATA+GGG - chr8.3:26085158-26085177 MS.gene87403:CDS 25.0%
!! TGATTTTGCTCTTAAAAATG+AGG - chr8.3:26085968-26085987 MS.gene87403:CDS 25.0%
!!! GATTACTTATTTTGAAGAAG+AGG - chr8.3:26085443-26085462 MS.gene87403:CDS 25.0%
AAATAAAACTCCTCCGTAAA+AGG + chr8.3:26085543-26085562 None:intergenic 30.0%
AAGAGAACTAATAACCCTAT+TGG + chr8.3:26085175-26085194 None:intergenic 30.0%
AATAAAACTCCTCCGTAAAA+GGG + chr8.3:26085542-26085561 None:intergenic 30.0%
GAATCAAGTGATTGTTAAGT+CGG - chr8.3:26085419-26085438 MS.gene87403:CDS 30.0%
GATTTGCAATTTCTTCAACA+TGG + chr8.3:26085598-26085617 None:intergenic 30.0%
GCTAATGATTGCACAAAAAT+GGG - chr8.3:26085051-26085070 MS.gene87403:CDS 30.0%
TCATCAAAACTTTGCAAGTT+TGG + chr8.3:26085135-26085154 None:intergenic 30.0%
TGCTAATGATTGCACAAAAA+TGG - chr8.3:26085050-26085069 MS.gene87403:CDS 30.0%
TGGTACTTACATTTCATTGA+AGG - chr8.3:26085878-26085897 MS.gene87403:CDS 30.0%
! GTATTAAAGAGCATACCAAT+AGG - chr8.3:26085157-26085176 MS.gene87403:CDS 30.0%
!! ACTTTTTAAGGGATATGAGA+AGG - chr8.3:26085815-26085834 MS.gene87403:CDS 30.0%
!! TTAGGAATTTTAAGGATGCT+GGG - chr8.3:26085514-26085533 MS.gene87403:CDS 30.0%
!!! AAGTTATTTTGGATTGCAAG+AGG - chr8.3:26085283-26085302 MS.gene87403:CDS 30.0%
!!! AGAAACTTTTTCAGGAACAT+TGG - chr8.3:26086031-26086050 MS.gene87403:CDS 30.0%
!!! AGTTATTTTGGATTGCAAGA+GGG - chr8.3:26085284-26085303 MS.gene87403:CDS 30.0%
!!! GGTGTCAAATGCAATTTTTT+TGG - chr8.3:26085668-26085687 MS.gene87403:CDS 30.0%
AATTACAAAACTCCAACGTC+TGG + chr8.3:26085368-26085387 None:intergenic 35.0%
AGTTGAAATCACTCAAGTCA+TGG - chr8.3:26085640-26085659 MS.gene87403:CDS 35.0%
ATATGAAGCTGCATGTATCA+AGG - chr8.3:26085107-26085126 MS.gene87403:CDS 35.0%
CTCTTGAGAAATGTTTGAGA+CGG - chr8.3:26085388-26085407 MS.gene87403:CDS 35.0%
GGATATGAGAAGGCTATAAA+GGG - chr8.3:26085825-26085844 MS.gene87403:CDS 35.0%
TGAAGGATTTGGACAACATT+CGG - chr8.3:26085895-26085914 MS.gene87403:CDS 35.0%
! AAACAAGGATTTGTTCGCAT+TGG - chr8.3:26085317-26085336 MS.gene87403:CDS 35.0%
! GCAAGAGCTTAGGAATTTTA+AGG - chr8.3:26085506-26085525 MS.gene87403:CDS 35.0%
! GTTATTAGTTCTCTTGCTAC+TGG - chr8.3:26085180-26085199 MS.gene87403:CDS 35.0%
! TCTTGAGAAATGTTTGAGAC+GGG - chr8.3:26085389-26085408 MS.gene87403:CDS 35.0%
! TTGAAGAAGAGGTGCAAAAT+GGG - chr8.3:26085454-26085473 MS.gene87403:CDS 35.0%
! TTTGAAGAAGAGGTGCAAAA+TGG - chr8.3:26085453-26085472 MS.gene87403:CDS 35.0%
!! CTTAGGAATTTTAAGGATGC+TGG - chr8.3:26085513-26085532 MS.gene87403:CDS 35.0%
!! TAGGAATTTTAAGGATGCTG+GGG - chr8.3:26085515-26085534 MS.gene87403:CDS 35.0%
!!! ATGGTGCAACTCACTTTTTA+AGG - chr8.3:26085803-26085822 MS.gene87403:CDS 35.0%
!!! TGGTGCAACTCACTTTTTAA+GGG - chr8.3:26085804-26085823 MS.gene87403:CDS 35.0%
!!! TTTTTTGGCTGCGTTTGTTT+CGG - chr8.3:26085683-26085702 MS.gene87403:CDS 35.0%
AATCACTCAAGTCATGGAAG+AGG - chr8.3:26085646-26085665 MS.gene87403:CDS 40.0%
AGAAGGCTATAAAGGGACAA+AGG - chr8.3:26085832-26085851 MS.gene87403:CDS 40.0%
AGGGAATTCAAGCAAGATGA+TGG - chr8.3:26085023-26085042 MS.gene87403:CDS 40.0%
ATCACTCAAGTCATGGAAGA+GGG - chr8.3:26085647-26085666 MS.gene87403:CDS 40.0%
GAAGGATTTGGACAACATTC+GGG - chr8.3:26085896-26085915 MS.gene87403:CDS 40.0%
GAAGGCTATAAAGGGACAAA+GGG - chr8.3:26085833-26085852 MS.gene87403:CDS 40.0%
GATTGCAAGAGGGATATATG+GGG - chr8.3:26085294-26085313 MS.gene87403:CDS 40.0%
GGATTGCAAGAGGGATATAT+GGG - chr8.3:26085293-26085312 MS.gene87403:CDS 40.0%
GGGATATGAGAAGGCTATAA+AGG - chr8.3:26085824-26085843 MS.gene87403:CDS 40.0%
TCGGGTGCTTACAAACAAAT+TGG - chr8.3:26085914-26085933 MS.gene87403:CDS 40.0%
TGAAGACATTGCAAGAGCTT+AGG - chr8.3:26085496-26085515 MS.gene87403:CDS 40.0%
TGATAATAGTCTCCAGACGT+TGG - chr8.3:26085353-26085372 MS.gene87403:CDS 40.0%
TGGATTGCAAGAGGGATATA+TGG - chr8.3:26085292-26085311 MS.gene87403:CDS 40.0%
! TTGCTACTGGTGTTGAAGTT+AGG - chr8.3:26085193-26085212 MS.gene87403:CDS 40.0%
!!! TTCGGTGTTGATTTTCTCTG+TGG - chr8.3:26085701-26085720 MS.gene87403:CDS 40.0%
GAGGGATATATGGGGAAACA+AGG - chr8.3:26085302-26085321 MS.gene87403:CDS 45.0%
GTAGTTAGCTCAATGCAGCT+TGG - chr8.3:26085858-26085877 MS.gene87403:CDS 45.0%
GTCGACAGATAAGAAAAGCG+AGG - chr8.3:26086084-26086103 MS.gene87403:CDS 45.0%
! AGGTGCAAAATGGGCTTGAA+GGG - chr8.3:26085463-26085482 MS.gene87403:CDS 45.0%
! TCAATCGGTTTATACGCAGC+AGG - chr8.3:26085569-26085588 MS.gene87403:CDS 45.0%
CATTCGGGTCGGTTGGAAAA+TGG - chr8.3:26085784-26085803 MS.gene87403:CDS 50.0%
CGCAGCCAAAGCTGTTACAA+CGG + chr8.3:26085751-26085770 None:intergenic 50.0%
GCAGCCAAAGCTGTTACAAC+GGG + chr8.3:26085750-26085769 None:intergenic 50.0%
! CATTTTCCAACCGACCCGAA+TGG + chr8.3:26085786-26085805 None:intergenic 50.0%
! CGTTGTAACAGCTTTGGCTG+CGG - chr8.3:26085749-26085768 MS.gene87403:CDS 50.0%
! GAGGTGCAAAATGGGCTTGA+AGG - chr8.3:26085462-26085481 MS.gene87403:CDS 50.0%
! GGTGCAAAATGGGCTTGAAG+GGG - chr8.3:26085464-26085483 MS.gene87403:CDS 50.0%
! GTGCAAAATGGGCTTGAAGG+GGG - chr8.3:26085465-26085484 MS.gene87403:CDS 50.0%
GCCAAAGCTGTTACAACGGG+CGG + chr8.3:26085747-26085766 None:intergenic 55.0%
GTTGGCTGTTCCATTCGGGT+CGG - chr8.3:26085773-26085792 MS.gene87403:CDS 55.0%
TCCGCCCGTTGTAACAGCTT+TGG - chr8.3:26085743-26085762 MS.gene87403:CDS 55.0%
! GGATGCTGGGGACCCTTTTA+CGG - chr8.3:26085527-26085546 MS.gene87403:CDS 55.0%
GCTGTTCCATTCGGGTCGGT+TGG - chr8.3:26085777-26085796 MS.gene87403:CDS 60.0%
! AACAGCTTTGGCTGCGGCGT+TGG - chr8.3:26085755-26085774 MS.gene87403:CDS 60.0%
! TGCTGGGGACCCTTTTACGG+AGG - chr8.3:26085530-26085549 MS.gene87403:CDS 60.0%
!! CGGCGTTGGCTGTTCCATTC+GGG - chr8.3:26085769-26085788 MS.gene87403:CDS 60.0%
!! GCGGCGTTGGCTGTTCCATT+CGG - chr8.3:26085768-26085787 MS.gene87403:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 26085018 26086145 26085018 ID=MS.gene87403
chr8.3 mRNA 26085018 26086145 26085018 ID=MS.gene87403.t1;Parent=MS.gene87403
chr8.3 exon 26085018 26086145 26085018 ID=MS.gene87403.t1.exon1;Parent=MS.gene87403.t1
chr8.3 CDS 26085018 26086145 26085018 ID=cds.MS.gene87403.t1;Parent=MS.gene87403.t1
Gene Sequence

>MS.gene87403

ATGGGAGGGAATTCAAGCAAGATGATGGTTGATGCTAATGATTGCACAAAAATGGGTACTCATTCTGTGTATGCAGCTGATTTAAGCTCATATGAAGCTGCATGTATCAAGGATCCAAACTTGCAAAGTTTTGATGAGAGTATTAAAGAGCATACCAATAGGGTTATTAGTTCTCTTGCTACTGGTGTTGAAGTTAGGTCTTTATCTTTCGATTCGCTGCGAGAAGTTACTGATAGTTTGCTTGAGATGAATCATGAAGTTGTTAAAGTTATTTTGGATTGCAAGAGGGATATATGGGGAAACAAGGATTTGTTCGCATTGGTTAATGATTATTTTGATAATAGTCTCCAGACGTTGGAGTTTTGTAATTCTCTTGAGAAATGTTTGAGACGGGCACGCGAGAATCAAGTGATTGTTAAGTCGGTGATTACTTATTTTGAAGAAGAGGTGCAAAATGGGCTTGAAGGGGGAACTTGTGTGAAGACATTGCAAGAGCTTAGGAATTTTAAGGATGCTGGGGACCCTTTTACGGAGGAGTTTTATTTGTTGTTTCAATCGGTTTATACGCAGCAGGCAACCATGTTGAAGAAATTGCAAATCAGAAAGAGAAAGCTTGATAAGAAGTTGAAATCACTCAAGTCATGGAAGAGGGTGTCAAATGCAATTTTTTTGGCTGCGTTTGTTTCGGTGTTGATTTTCTCTGTGGTTGCAGCTTCTATTGCTGCTCCGCCCGTTGTAACAGCTTTGGCTGCGGCGTTGGCTGTTCCATTCGGGTCGGTTGGAAAATGGTGCAACTCACTTTTTAAGGGATATGAGAAGGCTATAAAGGGACAAAGGGAAGTAGTTAGCTCAATGCAGCTTGGTACTTACATTTCATTGAAGGATTTGGACAACATTCGGGTGCTTACAAACAAATTGGAACTACAGCTTGAATCGTTATTGCAGAATGCTGATTTTGCTCTTAAAAATGAGGATGTGATAAAGCTTGCAATCGATGAGATTAAGAAGAATATAGAAACTTTTTCAGGAACATTGGAAACTCTAAGTGCAAATGCTGATAAATGCAGTCGACAGATAAGAAAAGCGAGGACAGTCGTTATACAAAATATAATCAAAAGACCTGATTGA

Protein sequence

>MS.gene87403.t1

MGGNSSKMMVDANDCTKMGTHSVYAADLSSYEAACIKDPNLQSFDESIKEHTNRVISSLATGVEVRSLSFDSLREVTDSLLEMNHEVVKVILDCKRDIWGNKDLFALVNDYFDNSLQTLEFCNSLEKCLRRARENQVIVKSVITYFEEEVQNGLEGGTCVKTLQELRNFKDAGDPFTEEFYLLFQSVYTQQATMLKKLQIRKRKLDKKLKSLKSWKRVSNAIFLAAFVSVLIFSVVAASIAAPPVVTALAAALAVPFGSVGKWCNSLFKGYEKAIKGQREVVSSMQLGTYISLKDLDNIRVLTNKLELQLESLLQNADFALKNEDVIKLAIDEIKKNIETFSGTLETLSANADKCSRQIRKARTVVIQNIIKRPD