Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066513.t1 | XP_003603170.1 | 100 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 8.30E-53 | 216.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066513.t1 | G7J8B4 | 100.0 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 5.9e-53 | 216.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene066513 | 0.810225 | 1.28E-50 | -1.69E-46 |
| MS.gene04920 | MS.gene066513 | 0.812746 | 3.63E-51 | -1.69E-46 |
| MS.gene049254 | MS.gene066513 | 0.849234 | 3.69E-60 | -1.69E-46 |
| MS.gene049283 | MS.gene066513 | 0.863144 | 3.07E-64 | -1.69E-46 |
| MS.gene049343 | MS.gene066513 | 0.801759 | 7.74E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene066513 | -0.813944 | 1.98E-51 | -1.69E-46 |
| MS.gene049499 | MS.gene066513 | 0.835268 | 1.85E-56 | -1.69E-46 |
| MS.gene049567 | MS.gene066513 | 0.807643 | 4.57E-50 | -1.69E-46 |
| MS.gene049652 | MS.gene066513 | 0.850023 | 2.22E-60 | -1.69E-46 |
| MS.gene049680 | MS.gene066513 | 0.829615 | 4.66E-55 | -1.69E-46 |
| MS.gene049983 | MS.gene066513 | 0.82479 | 6.67E-54 | -1.69E-46 |
| MS.gene050559 | MS.gene066513 | 0.838514 | 2.75E-57 | -1.69E-46 |
| MS.gene050561 | MS.gene066513 | 0.83028 | 3.21E-55 | -1.69E-46 |
| MS.gene050562 | MS.gene066513 | 0.830297 | 3.18E-55 | -1.69E-46 |
| MS.gene050571 | MS.gene066513 | 0.800088 | 1.70E-48 | -1.69E-46 |
| MS.gene05059 | MS.gene066513 | 0.802074 | 6.67E-49 | -1.69E-46 |
| MS.gene051332 | MS.gene066513 | 0.801934 | 7.12E-49 | -1.69E-46 |
| MS.gene051492 | MS.gene066513 | 0.808264 | 3.37E-50 | -1.69E-46 |
| MS.gene051637 | MS.gene066513 | 0.814914 | 1.21E-51 | -1.69E-46 |
| MS.gene051699 | MS.gene066513 | 0.849292 | 3.55E-60 | -1.69E-46 |
| MS.gene051744 | MS.gene066513 | 0.819185 | 1.33E-52 | -1.69E-46 |
| MS.gene051831 | MS.gene066513 | 0.806185 | 9.30E-50 | -1.69E-46 |
| MS.gene051832 | MS.gene066513 | 0.846473 | 2.12E-59 | -1.69E-46 |
| MS.gene051848 | MS.gene066513 | -0.811498 | 6.79E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene066513 | 0.826649 | 2.42E-54 | -1.69E-46 |
| MS.gene052037 | MS.gene066513 | 0.805528 | 1.28E-49 | -1.69E-46 |
| MS.gene052118 | MS.gene066513 | 0.818874 | 1.56E-52 | -1.69E-46 |
| MS.gene052493 | MS.gene066513 | 0.819488 | 1.13E-52 | -1.69E-46 |
| MS.gene052533 | MS.gene066513 | 0.843363 | 1.47E-58 | -1.69E-46 |
| MS.gene052534 | MS.gene066513 | 0.814426 | 1.55E-51 | -1.69E-46 |
| MS.gene052685 | MS.gene066513 | 0.829672 | 4.52E-55 | -1.69E-46 |
| MS.gene052686 | MS.gene066513 | 0.817275 | 3.59E-52 | -1.69E-46 |
| MS.gene052717 | MS.gene066513 | 0.803887 | 2.81E-49 | -1.69E-46 |
| MS.gene052855 | MS.gene066513 | 0.805432 | 1.34E-49 | -1.69E-46 |
| MS.gene052889 | MS.gene066513 | 0.810668 | 1.03E-50 | -1.69E-46 |
| MS.gene05308 | MS.gene066513 | 0.800258 | 1.57E-48 | -1.69E-46 |
| MS.gene053094 | MS.gene066513 | 0.869208 | 3.68E-66 | -1.69E-46 |
| MS.gene053298 | MS.gene066513 | 0.847807 | 9.15E-60 | -1.69E-46 |
| MS.gene053340 | MS.gene066513 | 0.831743 | 1.40E-55 | -1.69E-46 |
| MS.gene053963 | MS.gene066513 | 0.882869 | 7.25E-71 | -1.69E-46 |
| MS.gene053995 | MS.gene066513 | 0.80559 | 1.24E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene066513 | 0.816555 | 5.21E-52 | -1.69E-46 |
| MS.gene054222 | MS.gene066513 | 0.877409 | 6.43E-69 | -1.69E-46 |
| MS.gene05439 | MS.gene066513 | 0.802918 | 4.47E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene066513 | 0.811558 | 6.59E-51 | -1.69E-46 |
| MS.gene054576 | MS.gene066513 | 0.84065 | 7.65E-58 | -1.69E-46 |
| MS.gene054684 | MS.gene066513 | 0.81492 | 1.20E-51 | -1.69E-46 |
| MS.gene054892 | MS.gene066513 | 0.865923 | 4.15E-65 | -1.69E-46 |
| MS.gene054893 | MS.gene066513 | 0.860366 | 2.17E-63 | -1.69E-46 |
| MS.gene054957 | MS.gene066513 | 0.829087 | 6.26E-55 | -1.69E-46 |
| MS.gene055141 | MS.gene066513 | 0.825409 | 4.76E-54 | -1.69E-46 |
| MS.gene055147 | MS.gene066513 | 0.835996 | 1.21E-56 | -1.69E-46 |
| MS.gene055148 | MS.gene066513 | 0.823651 | 1.23E-53 | -1.69E-46 |
| MS.gene055149 | MS.gene066513 | 0.831092 | 2.03E-55 | -1.69E-46 |
| MS.gene055150 | MS.gene066513 | 0.828444 | 8.96E-55 | -1.69E-46 |
| MS.gene055226 | MS.gene066513 | 0.82332 | 1.48E-53 | -1.69E-46 |
| MS.gene055229 | MS.gene066513 | 0.842034 | 3.31E-58 | -1.69E-46 |
| MS.gene055724 | MS.gene066513 | 0.833841 | 4.23E-56 | -1.69E-46 |
| MS.gene055801 | MS.gene066513 | 0.812392 | 4.34E-51 | -1.69E-46 |
| MS.gene05624 | MS.gene066513 | 0.837135 | 6.21E-57 | -1.69E-46 |
| MS.gene056315 | MS.gene066513 | 0.828856 | 7.12E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene066513 | 0.801783 | 7.65E-49 | -1.69E-46 |
| MS.gene056677 | MS.gene066513 | 0.849343 | 3.44E-60 | -1.69E-46 |
| MS.gene056699 | MS.gene066513 | 0.865818 | 4.48E-65 | -1.69E-46 |
| MS.gene056779 | MS.gene066513 | 0.801531 | 8.62E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene066513 | 0.833933 | 4.01E-56 | -1.69E-46 |
| MS.gene056973 | MS.gene066513 | 0.802438 | 5.61E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene066513 | 0.82866 | 7.94E-55 | -1.69E-46 |
| MS.gene057034 | MS.gene066513 | 0.800352 | 1.50E-48 | -1.69E-46 |
| MS.gene057071 | MS.gene066513 | 0.812934 | 3.30E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene066513 | 0.864857 | 8.99E-65 | -1.69E-46 |
| MS.gene057155 | MS.gene066513 | 0.839654 | 1.39E-57 | -1.69E-46 |
| MS.gene057208 | MS.gene066513 | 0.842823 | 2.04E-58 | -1.69E-46 |
| MS.gene057622 | MS.gene066513 | 0.814739 | 1.32E-51 | -1.69E-46 |
| MS.gene057999 | MS.gene066513 | 0.808946 | 2.41E-50 | -1.69E-46 |
| MS.gene058159 | MS.gene066513 | 0.840142 | 1.04E-57 | -1.69E-46 |
| MS.gene058279 | MS.gene066513 | 0.866656 | 2.43E-65 | -1.69E-46 |
| MS.gene058592 | MS.gene066513 | 0.878062 | 3.80E-69 | -1.69E-46 |
| MS.gene058635 | MS.gene066513 | 0.810919 | 9.07E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene066513 | 0.803073 | 4.15E-49 | -1.69E-46 |
| MS.gene05878 | MS.gene066513 | 0.803666 | 3.13E-49 | -1.69E-46 |
| MS.gene058897 | MS.gene066513 | 0.809025 | 2.32E-50 | -1.69E-46 |
| MS.gene058979 | MS.gene066513 | 0.800932 | 1.14E-48 | -1.69E-46 |
| MS.gene059051 | MS.gene066513 | 0.844645 | 6.65E-59 | -1.69E-46 |
| MS.gene059170 | MS.gene066513 | 0.815429 | 9.29E-52 | -1.69E-46 |
| MS.gene05952 | MS.gene066513 | 0.885975 | 5.11E-72 | -1.69E-46 |
| MS.gene05956 | MS.gene066513 | 0.808244 | 3.41E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene066513 | 0.843189 | 1.63E-58 | -1.69E-46 |
| MS.gene05985 | MS.gene066513 | -0.88784 | 1.00E-72 | -1.69E-46 |
| MS.gene059901 | MS.gene066513 | -0.820437 | 6.86E-53 | -1.69E-46 |
| MS.gene059935 | MS.gene066513 | 0.822362 | 2.47E-53 | -1.69E-46 |
| MS.gene059965 | MS.gene066513 | 0.820347 | 7.20E-53 | -1.69E-46 |
| MS.gene060012 | MS.gene066513 | 0.860677 | 1.75E-63 | -1.69E-46 |
| MS.gene060244 | MS.gene066513 | 0.802371 | 5.79E-49 | -1.69E-46 |
| MS.gene060257 | MS.gene066513 | 0.808699 | 2.72E-50 | -1.69E-46 |
| MS.gene060258 | MS.gene066513 | 0.800168 | 1.64E-48 | -1.69E-46 |
| MS.gene060259 | MS.gene066513 | 0.8017 | 7.96E-49 | -1.69E-46 |
| MS.gene060338 | MS.gene066513 | 0.803214 | 3.88E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene066513 | 0.838538 | 2.71E-57 | -1.69E-46 |
| MS.gene060373 | MS.gene066513 | 0.804233 | 2.38E-49 | -1.69E-46 |
| MS.gene060412 | MS.gene066513 | 0.859126 | 5.13E-63 | -1.69E-46 |
| MS.gene060520 | MS.gene066513 | 0.802011 | 6.87E-49 | -1.69E-46 |
| MS.gene060527 | MS.gene066513 | 0.830979 | 2.16E-55 | -1.69E-46 |
| MS.gene060697 | MS.gene066513 | 0.838581 | 2.64E-57 | -1.69E-46 |
| MS.gene060716 | MS.gene066513 | 0.806364 | 8.53E-50 | -1.69E-46 |
| MS.gene060777 | MS.gene066513 | 0.828218 | 1.02E-54 | -1.69E-46 |
| MS.gene060840 | MS.gene066513 | 0.843257 | 1.57E-58 | -1.69E-46 |
| MS.gene060879 | MS.gene066513 | 0.80682 | 6.83E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene066513 | 0.807166 | 5.77E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene066513 | 0.811584 | 6.51E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene066513 | 0.830493 | 2.85E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene066513 | 0.845519 | 3.86E-59 | -1.69E-46 |
| MS.gene061047 | MS.gene066513 | 0.832345 | 9.97E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene066513 | 0.806966 | 6.36E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene066513 | 0.855331 | 6.78E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066513.t1 | MTR_3g104640 | 100.000 | 110 | 0 | 0 | 1 | 110 | 1 | 110 | 1.63e-74 | 216 |
| MS.gene066513.t1 | MTR_4g125990 | 55.906 | 127 | 35 | 5 | 1 | 110 | 1 | 123 | 8.48e-35 | 116 |
| MS.gene066513.t1 | MTR_1g015810 | 67.241 | 116 | 30 | 5 | 1 | 108 | 1 | 116 | 6.41e-32 | 108 |
| MS.gene066513.t1 | MTR_2g099630 | 49.541 | 109 | 44 | 4 | 6 | 109 | 4 | 106 | 4.69e-26 | 93.6 |
| MS.gene066513.t1 | MTR_8g018320 | 50.495 | 101 | 49 | 1 | 6 | 106 | 4 | 103 | 6.89e-22 | 83.2 |
| MS.gene066513.t1 | MTR_8g018360 | 50.000 | 102 | 50 | 1 | 6 | 107 | 4 | 104 | 7.16e-21 | 82.8 |
| MS.gene066513.t1 | MTR_7g109290 | 46.667 | 60 | 32 | 0 | 51 | 110 | 32 | 91 | 5.29e-14 | 62.4 |
| MS.gene066513.t1 | MTR_1g098310 | 57.407 | 54 | 20 | 1 | 56 | 109 | 35 | 85 | 2.54e-13 | 60.8 |
| MS.gene066513.t1 | MTR_1g087730 | 42.373 | 59 | 34 | 0 | 52 | 110 | 48 | 106 | 9.62e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066513.t1 | AT5G11970 | 59.091 | 110 | 39 | 3 | 1 | 110 | 1 | 104 | 1.79e-35 | 117 |
| MS.gene066513.t1 | AT2G19460 | 45.455 | 121 | 46 | 5 | 1 | 107 | 1 | 115 | 4.13e-21 | 81.6 |
| MS.gene066513.t1 | AT2G19460 | 45.455 | 121 | 46 | 5 | 1 | 107 | 53 | 167 | 1.95e-20 | 81.3 |
| MS.gene066513.t1 | AT2G47480 | 51.613 | 62 | 27 | 1 | 50 | 108 | 47 | 108 | 5.75e-15 | 65.5 |
| MS.gene066513.t1 | AT3G13910 | 58.621 | 58 | 23 | 1 | 45 | 101 | 40 | 97 | 2.48e-14 | 63.9 |
| MS.gene066513.t1 | AT3G13910 | 58.621 | 58 | 23 | 1 | 45 | 101 | 40 | 97 | 5.38e-14 | 63.2 |
| MS.gene066513.t1 | AT1G72720 | 62.222 | 45 | 17 | 0 | 65 | 109 | 81 | 125 | 6.28e-14 | 63.5 |
| MS.gene066513.t1 | AT3G62640 | 51.111 | 45 | 22 | 0 | 65 | 109 | 66 | 110 | 6.18e-13 | 60.5 |
| MS.gene066513.t1 | AT3G05725 | 35.115 | 131 | 55 | 7 | 1 | 109 | 1 | 123 | 1.61e-12 | 59.7 |
| MS.gene066513.t1 | AT4G09890 | 53.846 | 39 | 18 | 0 | 66 | 104 | 39 | 77 | 5.58e-11 | 54.7 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTACTCACAGGTGGTTTA+TGG | 0.228498 | 3.3:-86035577 | MS.gene066513:CDS |
| CTCTTCTTCCTCTGAAGTTC+AGG | 0.289847 | 3.3:+86035679 | None:intergenic |
| AGCTTTCTATCTGCATCATT+TGG | 0.373432 | 3.3:+86035842 | None:intergenic |
| TATAAAATGTATTCTGTTGA+AGG | 0.385236 | 3.3:-86035646 | MS.gene066513:CDS |
| CTAAGATCATAAGGCTTAGA+TGG | 0.404348 | 3.3:+86035811 | None:intergenic |
| ATGCAGATAGAAAGCTACTA+TGG | 0.407129 | 3.3:-86035835 | MS.gene066513:CDS |
| TATCTGCATCATTTGGCCAT+TGG | 0.440208 | 3.3:+86035849 | None:intergenic |
| CTATAGCTTCTAAGATCATA+AGG | 0.444134 | 3.3:+86035802 | None:intergenic |
| CTGAACTTCAGAGGAAGAAG+AGG | 0.478478 | 3.3:-86035678 | MS.gene066513:CDS |
| TTCTTCAACTTCAAATCCTT+AGG | 0.481282 | 3.3:+86035757 | None:intergenic |
| AAGGATTTGAAGTTGAAGAA+AGG | 0.511800 | 3.3:-86035754 | MS.gene066513:CDS |
| CTTAGGAGTTTGTTGTACAT+AGG | 0.522202 | 3.3:+86035774 | None:intergenic |
| CTATGTACAACAAACTCCTA+AGG | 0.555597 | 3.3:-86035773 | MS.gene066513:CDS |
| TCTGTTGAAGGCAAAGTCAA+AGG | 0.571369 | 3.3:-86035634 | MS.gene066513:CDS |
| CTTTCAGAAAGAGTTTCAGA+TGG | 0.576426 | 3.3:-86035609 | MS.gene066513:CDS |
| ACTCACAGGTGGTTTATGGT+TGG | 0.580184 | 3.3:-86035573 | MS.gene066513:CDS |
| TGTCTGATCCTGAACTTCAG+AGG | 0.591643 | 3.3:-86035687 | MS.gene066513:CDS |
| AGATCAAAATCTTATGCCAA+TGG | 0.604385 | 3.3:-86035865 | MS.gene066513:CDS |
| GCTTAAGAACAAGTACTCAC+AGG | 0.640169 | 3.3:-86035587 | MS.gene066513:CDS |
| TGAACTTCAGAGGAAGAAGA+GGG | 0.657165 | 3.3:-86035677 | MS.gene066513:CDS |
| TAAGAACAAGTACTCACAGG+TGG | 0.685797 | 3.3:-86035584 | MS.gene066513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAAAATGTATTCTGTTGA+AGG | - | chr3.3:86035804-86035823 | MS.gene066513:CDS | 20.0% |
| AGATCAAAATCTTATGCCAA+TGG | - | chr3.3:86035585-86035604 | MS.gene066513:CDS | 30.0% | |
| TTCTTCAACTTCAAATCCTT+AGG | + | chr3.3:86035696-86035715 | None:intergenic | 30.0% | |
| ! | AAAGGTAAAAGCTTTTCATC+TGG | - | chr3.3:86035714-86035733 | MS.gene066513:CDS | 30.0% |
| ! | AAGGATTTGAAGTTGAAGAA+AGG | - | chr3.3:86035696-86035715 | MS.gene066513:CDS | 30.0% |
| !! | CTATAGCTTCTAAGATCATA+AGG | + | chr3.3:86035651-86035670 | None:intergenic | 30.0% |
| AGCTTTCTATCTGCATCATT+TGG | + | chr3.3:86035611-86035630 | None:intergenic | 35.0% | |
| ATGCAGATAGAAAGCTACTA+TGG | - | chr3.3:86035615-86035634 | MS.gene066513:CDS | 35.0% | |
| CTAAGATCATAAGGCTTAGA+TGG | + | chr3.3:86035642-86035661 | None:intergenic | 35.0% | |
| CTATGTACAACAAACTCCTA+AGG | - | chr3.3:86035677-86035696 | MS.gene066513:CDS | 35.0% | |
| CTTTCAGAAAGAGTTTCAGA+TGG | - | chr3.3:86035841-86035860 | MS.gene066513:CDS | 35.0% | |
| !! | CTTAGGAGTTTGTTGTACAT+AGG | + | chr3.3:86035679-86035698 | None:intergenic | 35.0% |
| AAGTACTCACAGGTGGTTTA+TGG | - | chr3.3:86035873-86035892 | MS.gene066513:CDS | 40.0% | |
| GCTTAAGAACAAGTACTCAC+AGG | - | chr3.3:86035863-86035882 | MS.gene066513:CDS | 40.0% | |
| TAAGAACAAGTACTCACAGG+TGG | - | chr3.3:86035866-86035885 | MS.gene066513:CDS | 40.0% | |
| TATCTGCATCATTTGGCCAT+TGG | + | chr3.3:86035604-86035623 | None:intergenic | 40.0% | |
| TCTGTTGAAGGCAAAGTCAA+AGG | - | chr3.3:86035816-86035835 | MS.gene066513:CDS | 40.0% | |
| TGAACTTCAGAGGAAGAAGA+GGG | - | chr3.3:86035773-86035792 | MS.gene066513:CDS | 40.0% | |
| ACTCACAGGTGGTTTATGGT+TGG | - | chr3.3:86035877-86035896 | MS.gene066513:CDS | 45.0% | |
| CTGAACTTCAGAGGAAGAAG+AGG | - | chr3.3:86035772-86035791 | MS.gene066513:CDS | 45.0% | |
| TGTCTGATCCTGAACTTCAG+AGG | - | chr3.3:86035763-86035782 | MS.gene066513:CDS | 45.0% | |
| !! | CTCTTCTTCCTCTGAAGTTC+AGG | + | chr3.3:86035774-86035793 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 86035570 | 86035902 | 86035570 | ID=MS.gene066513 |
| chr3.3 | mRNA | 86035570 | 86035902 | 86035570 | ID=MS.gene066513.t1;Parent=MS.gene066513 |
| chr3.3 | exon | 86035570 | 86035902 | 86035570 | ID=MS.gene066513.t1.exon1;Parent=MS.gene066513.t1 |
| chr3.3 | CDS | 86035570 | 86035902 | 86035570 | ID=cds.MS.gene066513.t1;Parent=MS.gene066513.t1 |
| Gene Sequence |
| Protein sequence |
