AlfalfaGEDB | MS.gene052735

M. sativa cultivar XinJiangDaYe / MS.gene052735

KEGG: -
GO: -
PFAM: PF14223.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052735.t1	PNX91497.1	92.9	141	10	0	1	141	1	141	5.60E-67	264.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052735.t1	P10978	36.2	138	85	2	7	141	5	142	2.8e-19	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052735.t1	A0A2K3ML10	92.9	141	10	0	1	141	1	141	4.0e-67	264.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052735	MS.gene052736	0.937161	5.43E-98	-1.69E-46
MS.gene052735	MS.gene066511	0.816632	5.00E-52	-1.69E-46
MS.gene052735	MS.gene31193	0.815104	1.10E-51	-1.69E-46
MS.gene052735	MS.gene37330	0.818223	2.19E-52	-1.69E-46
MS.gene052735	MS.gene48776	0.802109	6.56E-49	-1.69E-46
MS.gene052735	MS.gene51267	0.831368	1.74E-55	-1.69E-46
MS.gene052735	MS.gene51268	0.811347	7.33E-51	-1.69E-46
MS.gene052735	MS.gene56791	0.815785	7.74E-52	-1.69E-46
MS.gene052735	MS.gene58134	0.801874	7.33E-49	-1.69E-46
MS.gene052735	MS.gene80894	0.81414	1.79E-51	-1.69E-46
MS.gene052735	MS.gene95335	0.81352	2.46E-51	-1.69E-46
MS.gene052735	MS.gene95337	0.824316	8.62E-54	-1.69E-46
MS.gene052735	MS.gene95338	0.84059	7.94E-58	-1.69E-46
MS.gene052735	MS.gene95339	0.802562	5.29E-49	-1.69E-46
MS.gene052735	MS.gene97974	0.828945	6.78E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052735.t1	MTR_2g039140	46.602	103	54	1	1	102	120	222	7.26e-20	85.9
MS.gene052735.t1	MTR_4g036430	89.744	39	4	0	1	39	31	69	1.50e-19	79.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052735.t1	AT3G29785	57.778	90	37	1	11	100	7	95	5.82e-32	113

Find 52 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATACCAACTGAACTTAAT+TGG	0.095422	2.1:-7415269	None:intergenic
GTGAGAACTACGATGATTTC+TGG	0.205326	2.1:-7415553	None:intergenic
TCATCATCCTTCATCGATTC+TGG	0.247368	2.1:-7415009	None:intergenic
TTCGCCTCTTGATTCTTTGC+TGG	0.253882	2.1:-7415642	None:intergenic
ACTACGATGATTTCTGGATT+TGG	0.314338	2.1:-7415547	None:intergenic
TCGCCTCTTGATTCTTTGCT+GGG	0.374564	2.1:-7415641	None:intergenic
ATGGTGCGGACTTTGGCTTT+TGG	0.377376	2.1:+7414923	MS.gene052735:CDS
GATACCAACTGAACTTAATT+GGG	0.381678	2.1:-7415268	None:intergenic
ATTTCTGGATTTGGATCGTC+TGG	0.389287	2.1:-7415538	None:intergenic
AGAAATTCAACCAGAGGAAA+TGG	0.417568	2.1:+7415498	MS.gene052735:CDS
GAGATCCGACGGAGAGAGTT+GGG	0.442215	2.1:+7415432	MS.gene052735:intron
GCAAATGTTGCTTCCACCTC+AGG	0.445655	2.1:+7415672	MS.gene052735:CDS
AAGTTCGATGGTGCGGACTT+TGG	0.452497	2.1:+7414916	MS.gene052735:CDS
TGAACTTTATTTGATGCTGA+AGG	0.474628	2.1:-7415162	None:intergenic
TTTACCACGTCCATTTCCTC+TGG	0.487326	2.1:-7415508	None:intergenic
TGAGGCGGTTGTTTACGCTT+CGG	0.506448	2.1:+7415190	MS.gene052735:CDS
CGCGTCACCTCCTCCTGAGG+TGG	0.515832	2.1:-7415685	None:intergenic
GTGTTGGAACTGTGGAAAGA+TGG	0.515954	2.1:+7415593	MS.gene052735:CDS
AACCACCGCTGGACTTATGA+AGG	0.535609	2.1:+7415116	MS.gene052735:CDS
AAAGAAACCAGAATCGATGA+AGG	0.539324	2.1:+7415002	MS.gene052735:CDS
AGAGATCCGACGGAGAGAGT+TGG	0.549236	2.1:+7415431	MS.gene052735:intron
CAAATAAAGTTCATTTGATG+AGG	0.550433	2.1:+7415172	MS.gene052735:CDS
TTTACGCTTCGGATGACAGA+AGG	0.562705	2.1:+7415201	MS.gene052735:CDS
GTGAAAATTGAAAAGTTCGA+TGG	0.563926	2.1:+7414904	MS.gene052735:CDS
CTCTGTATGCAATACTGAAG+AGG	0.567853	2.1:-7415466	None:intergenic
ACAACCCAATTAAGTTCAGT+TGG	0.568124	2.1:+7415264	MS.gene052735:CDS
GTTGCTTCCACCTCAGGAGG+AGG	0.568689	2.1:+7415678	MS.gene052735:CDS
ACTCGAAATCTATCGAGTGT+TGG	0.576612	2.1:+7415577	MS.gene052735:CDS
TGTTGGAACTGTGGAAAGAT+GGG	0.586945	2.1:+7415594	MS.gene052735:CDS
AAATGGACGTGGTAAATCAA+AGG	0.596335	2.1:+7415515	MS.gene052735:CDS
TTCTCAGTGAAGAGATCCGA+CGG	0.596721	2.1:+7415421	MS.gene052735:intron
GATGACAGAAGGCGCGTCAG+TGG	0.598117	2.1:+7415212	MS.gene052735:CDS
ATAAAGTTCATTTGATGAGG+CGG	0.598437	2.1:+7415175	MS.gene052735:CDS
CTTCTCGATAGACAAGCACT+TGG	0.601850	2.1:+7415039	MS.gene052735:CDS
TCTATCGAGTGTTGGAACTG+TGG	0.601883	2.1:+7415585	MS.gene052735:CDS
AATGTTGCTTCCACCTCAGG+AGG	0.603928	2.1:+7415675	MS.gene052735:CDS
AATCGATGAAGGATGATGAG+TGG	0.606445	2.1:+7415013	MS.gene052735:CDS
GCAAAAGAGAAAACCACCGC+TGG	0.607234	2.1:+7415105	MS.gene052735:CDS
AATTGAAAAGTTCGATGGTG+CGG	0.608192	2.1:+7414909	MS.gene052735:CDS
CAAGTGCTTGTCTATCGAGA+AGG	0.612004	2.1:-7415038	None:intergenic
TATGGCTGCAGACGAAGGAA+AGG	0.614378	2.1:+7414882	None:intergenic
ATTCACCCAACTCTCTCCGT+CGG	0.616565	2.1:-7415437	None:intergenic
GGAGTTATGGCTGCAGACGA+AGG	0.617375	2.1:+7414877	None:intergenic
AGCCTTCATAAGTCCAGCGG+TGG	0.623238	2.1:-7415118	None:intergenic
GAATCAAGAGGCGAAAGATG+AGG	0.623736	2.1:+7415650	MS.gene052735:CDS
CAACGCGTCACCTCCTCCTG+AGG	0.630580	2.1:-7415688	None:intergenic
TGGTATCGAATTTGACGATG+AGG	0.639552	2.1:+7415284	MS.gene052735:CDS
AGAGGAAGAAATTCAACCAG+AGG	0.643795	2.1:+7415492	MS.gene052735:CDS
TCAACCAGAGGAAATGGACG+TGG	0.665050	2.1:+7415504	MS.gene052735:CDS
GCTCCCAGCAAAGAATCAAG+AGG	0.671318	2.1:+7415638	MS.gene052735:CDS
GTATTGCATACAGAGTCAAG+AGG	0.682691	2.1:+7415474	MS.gene052735:CDS
AAGAGCCTTCATAAGTCCAG+CGG	0.707222	2.1:-7415121	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAAATAAAGTTCATTTGATG+AGG	+	chr2.1:7415172-7415191	MS.gene052735:CDS	25.0%
	ATAAAGTTCATTTGATGAGG+CGG	+	chr2.1:7415175-7415194	MS.gene052735:CDS	30.0%
	GATACCAACTGAACTTAATT+GGG	-	chr2.1:7415271-7415290	None:intergenic	30.0%
	GTGAAAATTGAAAAGTTCGA+TGG	+	chr2.1:7414904-7414923	MS.gene052735:CDS	30.0%
!	TGAACTTTATTTGATGCTGA+AGG	-	chr2.1:7415165-7415184	None:intergenic	30.0%
	AAAGAAACCAGAATCGATGA+AGG	+	chr2.1:7415002-7415021	MS.gene052735:CDS	35.0%
	AAATGGACGTGGTAAATCAA+AGG	+	chr2.1:7415515-7415534	MS.gene052735:CDS	35.0%
	AATTGAAAAGTTCGATGGTG+CGG	+	chr2.1:7414909-7414928	MS.gene052735:CDS	35.0%
	ACAACCCAATTAAGTTCAGT+TGG	+	chr2.1:7415264-7415283	MS.gene052735:CDS	35.0%
	AGAAATTCAACCAGAGGAAA+TGG	+	chr2.1:7415498-7415517	MS.gene052735:CDS	35.0%
	CGATACCAACTGAACTTAAT+TGG	-	chr2.1:7415272-7415291	None:intergenic	35.0%
	GTCACTTCAAAAATCAGTGT+AGG	+	chr2.1:7415616-7415635	MS.gene052735:CDS	35.0%
!	ACTACGATGATTTCTGGATT+TGG	-	chr2.1:7415550-7415569	None:intergenic	35.0%
!!	ATTTGATGATGTTCGTGATC+TGG	+	chr2.1:7415398-7415417	MS.gene052735:intron	35.0%
	AATCGATGAAGGATGATGAG+TGG	+	chr2.1:7415013-7415032	MS.gene052735:CDS	40.0%
	ACTCGAAATCTATCGAGTGT+TGG	+	chr2.1:7415577-7415596	MS.gene052735:CDS	40.0%
	AGAGGAAGAAATTCAACCAG+AGG	+	chr2.1:7415492-7415511	MS.gene052735:CDS	40.0%
	CTCTGTATGCAATACTGAAG+AGG	-	chr2.1:7415469-7415488	None:intergenic	40.0%
	GTATTGCATACAGAGTCAAG+AGG	+	chr2.1:7415474-7415493	MS.gene052735:CDS	40.0%
	GTGAGAACTACGATGATTTC+TGG	-	chr2.1:7415556-7415575	None:intergenic	40.0%
	TGTTGGAACTGTGGAAAGAT+GGG	+	chr2.1:7415594-7415613	MS.gene052735:CDS	40.0%
!	ATTTCTGGATTTGGATCGTC+TGG	-	chr2.1:7415541-7415560	None:intergenic	40.0%
!	TGGTATCGAATTTGACGATG+AGG	+	chr2.1:7415284-7415303	MS.gene052735:CDS	40.0%
!!	TCATCATCCTTCATCGATTC+TGG	-	chr2.1:7415012-7415031	None:intergenic	40.0%
!!	TCTGGTTTCTTTTCTGTGAG+AGG	-	chr2.1:7414994-7415013	None:intergenic	40.0%
	AAGAGCCTTCATAAGTCCAG+CGG	-	chr2.1:7415124-7415143	None:intergenic	45.0%
	CAAGTGCTTGTCTATCGAGA+AGG	-	chr2.1:7415041-7415060	None:intergenic	45.0%
	CTTCTCGATAGACAAGCACT+TGG	+	chr2.1:7415039-7415058	MS.gene052735:CDS	45.0%
	GAATCAAGAGGCGAAAGATG+AGG	+	chr2.1:7415650-7415669	MS.gene052735:CDS	45.0%
	GTGTTGGAACTGTGGAAAGA+TGG	+	chr2.1:7415593-7415612	MS.gene052735:CDS	45.0%
	TCGCCTCTTGATTCTTTGCT+GGG	-	chr2.1:7415644-7415663	None:intergenic	45.0%
	TGTCTTCCCTACCAGATAGT+TGG	+	chr2.1:7415325-7415344	MS.gene052735:intron	45.0%
	TTCGCCTCTTGATTCTTTGC+TGG	-	chr2.1:7415645-7415664	None:intergenic	45.0%
	TTCTCAGTGAAGAGATCCGA+CGG	+	chr2.1:7415421-7415440	MS.gene052735:intron	45.0%
	TTTACCACGTCCATTTCCTC+TGG	-	chr2.1:7415511-7415530	None:intergenic	45.0%
	TTTACGCTTCGGATGACAGA+AGG	+	chr2.1:7415201-7415220	MS.gene052735:CDS	45.0%
!!	TCTATCGAGTGTTGGAACTG+TGG	+	chr2.1:7415585-7415604	MS.gene052735:CDS	45.0%
	AACCACCGCTGGACTTATGA+AGG	+	chr2.1:7415116-7415135	MS.gene052735:CDS	50.0%
	AAGTTCGATGGTGCGGACTT+TGG	+	chr2.1:7414916-7414935	MS.gene052735:CDS	50.0%
	ACAGTAGCGCTCCAACTATC+TGG	-	chr2.1:7415339-7415358	None:intergenic	50.0%
	AGCGCTCCAACTATCTGGTA+GGG	-	chr2.1:7415334-7415353	None:intergenic	50.0%
	ATTCACCCAACTCTCTCCGT+CGG	-	chr2.1:7415440-7415459	None:intergenic	50.0%
	GCAAAAGAGAAAACCACCGC+TGG	+	chr2.1:7415105-7415124	MS.gene052735:CDS	50.0%
	GCTCCCAGCAAAGAATCAAG+AGG	+	chr2.1:7415638-7415657	MS.gene052735:CDS	50.0%
	TAGCGCTCCAACTATCTGGT+AGG	-	chr2.1:7415335-7415354	None:intergenic	50.0%
	TCAACCAGAGGAAATGGACG+TGG	+	chr2.1:7415504-7415523	MS.gene052735:CDS	50.0%
	TGAGGCGGTTGTTTACGCTT+CGG	+	chr2.1:7415190-7415209	MS.gene052735:CDS	50.0%
!	AATGTTGCTTCCACCTCAGG+AGG	+	chr2.1:7415675-7415694	MS.gene052735:CDS	50.0%
!	GCAAATGTTGCTTCCACCTC+AGG	+	chr2.1:7415672-7415691	MS.gene052735:CDS	50.0%
!	TAGTTGGAGCGCTACTGTCA+CGG	+	chr2.1:7415341-7415360	MS.gene052735:intron	50.0%
!!!	ATGGTGCGGACTTTGGCTTT+TGG	+	chr2.1:7414923-7414942	MS.gene052735:CDS	50.0%
	ACGGCTGTGAGTAGCTCATC+GGG	+	chr2.1:7415360-7415379	MS.gene052735:intron	55.0%
	AGAGATCCGACGGAGAGAGT+TGG	+	chr2.1:7415431-7415450	MS.gene052735:intron	55.0%
	AGCCTTCATAAGTCCAGCGG+TGG	-	chr2.1:7415121-7415140	None:intergenic	55.0%
	CACGGCTGTGAGTAGCTCAT+CGG	+	chr2.1:7415359-7415378	MS.gene052735:intron	55.0%
	GAGATCCGACGGAGAGAGTT+GGG	+	chr2.1:7415432-7415451	MS.gene052735:intron	55.0%
	GTTGCTTCCACCTCAGGAGG+AGG	+	chr2.1:7415678-7415697	MS.gene052735:CDS	60.0%
!	GATGACAGAAGGCGCGTCAG+TGG	+	chr2.1:7415212-7415231	MS.gene052735:CDS	60.0%
	CAACGCGTCACCTCCTCCTG+AGG	-	chr2.1:7415691-7415710	None:intergenic	65.0%
	CGCGTCACCTCCTCCTGAGG+TGG	-	chr2.1:7415688-7415707	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	7414883	7415711	7414883	ID=MS.gene052735
chr2.1	mRNA	7414883	7415711	7414883	ID=MS.gene052735.t1;Parent=MS.gene052735
chr2.1	exon	7414883	7415305	7414883	ID=MS.gene052735.t1.exon1;Parent=MS.gene052735.t1
chr2.1	CDS	7414883	7415305	7414883	ID=cds.MS.gene052735.t1;Parent=MS.gene052735.t1
chr2.1	exon	7415433	7415711	7415433	ID=MS.gene052735.t1.exon2;Parent=MS.gene052735.t1
chr2.1	CDS	7415433	7415711	7415433	ID=cds.MS.gene052735.t1;Parent=MS.gene052735.t1

Gene Sequence

>MS.gene052735

ATGGCTGCAGACGAAGGAAAGGTGAAAATTGAAAAGTTCGATGGTGCGGACTTTGGCTTTTGGAAGATGCAGATCGAAGATTATTTGTATCAGAAAAAGCTACATCAACCTCTCACAGAAAAGAAACCAGAATCGATGAAGGATGATGAGTGGAACCTTCTCGATAGACAAGCACTTGGTGTTGTGCGATTGTCGTTATCTCGAAATGTTGCTTTTAACATAGCAAAAGAGAAAACCACCGCTGGACTTATGAAGGCTCTTTCTAGCATGTATGAAAAGCCTTCAGCATCAAATAAAGTTCATTTGATGAGGCGGTTGTTTACGCTTCGGATGACAGAAGGCGCGTCAGTGGCGCAACATATCAATGAACTTAATATTGTCACAACCCAATTAAGTTCAGTTGGTATCGAATTTGACGATGAGGTACGAGCATTGATACTTTTGTCTTCCCTACCAGATAGTTGGAGCGCTACTGTCACGGCTGTGAGTAGCTCATCGGGAAGCAAAAAGATGAAATTTGATGATGTTCGTGATCTGGTTCTCAGTGAAGAGATCCGACGGAGAGAGTTGGGTGAATCATCATCCTCTTCAGTATTGCATACAGAGTCAAGAGGAAGAAATTCAACCAGAGGAAATGGACGTGGTAAATCAAAGGCCAGACGATCCAAATCCAGAAATCATCGTAGTTCTCACAACTCGAAATCTATCGAGTGTTGGAACTGTGGAAAGATGGGTCACTTCAAAAATCAGTGTAGGCTCCCAGCAAAGAATCAAGAGGCGAAAGATGAGGCAAATGTTGCTTCCACCTCAGGAGGAGGTGACGCGTTGA

Protein sequence

>MS.gene052735.t1

MAADEGKVKIEKFDGADFGFWKMQIEDYLYQKKLHQPLTEKKPESMKDDEWNLLDRQALGVVRLSLSRNVAFNIAKEKTTAGLMKALSSMYEKPSASNKVHLMRRLFTLRMTEGASVAQHINELNIVTTQLSSVGIEFDDERSDGESWVNHHPLQYCIQSQEEEIQPEEMDVVNQRPDDPNPEIIVVLTTRNLSSVGTVERWVTSKISVGSQQRIKRRKMRQMLLPPQEEVTR