AlfalfaGEDB | MS.gene052736

M. sativa cultivar XinJiangDaYe / MS.gene052736

KEGG: -
GO: -
PFAM: PF14223.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052736.t1	PNX91497.1	92.9	141	10	0	1	141	1	141	5.60E-67	264.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052736.t1	P10978	36.2	138	85	2	7	141	5	142	2.8e-19	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052736.t1	A0A2K3ML10	92.9	141	10	0	1	141	1	141	4.0e-67	264.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052735	MS.gene052736	0.937161	5.43E-98	-1.69E-46
MS.gene052736	MS.gene20018	0.801274	9.73E-49	-1.69E-46
MS.gene052736	MS.gene31193	0.827966	1.17E-54	-1.69E-46
MS.gene052736	MS.gene37330	0.824451	8.02E-54	-1.69E-46
MS.gene052736	MS.gene48776	0.805223	1.48E-49	-1.69E-46
MS.gene052736	MS.gene51255	0.805208	1.49E-49	-1.69E-46
MS.gene052736	MS.gene51267	0.832383	9.75E-56	-1.69E-46
MS.gene052736	MS.gene51268	0.825918	3.61E-54	-1.69E-46
MS.gene052736	MS.gene54925	0.818498	1.90E-52	-1.69E-46
MS.gene052736	MS.gene60478	0.803714	3.06E-49	-1.69E-46
MS.gene052736	MS.gene70750	0.808868	2.51E-50	-1.69E-46
MS.gene052736	MS.gene80894	0.809669	1.69E-50	-1.69E-46
MS.gene052736	MS.gene95334	0.802868	4.57E-49	-1.69E-46
MS.gene052736	MS.gene95335	0.826666	2.39E-54	-1.69E-46
MS.gene052736	MS.gene95337	0.828558	8.41E-55	-1.69E-46
MS.gene052736	MS.gene95338	0.846292	2.38E-59	-1.69E-46
MS.gene052736	MS.gene97974	0.830883	2.28E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052736.t1	MTR_2g039140	46.602	103	54	1	1	102	120	222	7.26e-20	85.9
MS.gene052736.t1	MTR_4g036430	89.744	39	4	0	1	39	31	69	1.50e-19	79.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052736.t1	AT3G29785	57.778	90	37	1	11	100	7	95	5.82e-32	113

Find 52 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGATACCAACTGAACTTAAT+TGG	0.095422	2.1:-7402579	None:intergenic
GTGAGAACTACGATGATTTC+TGG	0.205326	2.1:-7402863	None:intergenic
TCATCATCCTTCATCGATTC+TGG	0.247368	2.1:-7402319	None:intergenic
TTCGCCTCTTGATTCTTTGC+TGG	0.253882	2.1:-7402952	None:intergenic
ACTACGATGATTTCTGGATT+TGG	0.314338	2.1:-7402857	None:intergenic
TCGCCTCTTGATTCTTTGCT+GGG	0.374564	2.1:-7402951	None:intergenic
ATGGTGCGGACTTTGGCTTT+TGG	0.377376	2.1:+7402233	MS.gene052736:CDS
GATACCAACTGAACTTAATT+GGG	0.381678	2.1:-7402578	None:intergenic
ATTTCTGGATTTGGATCGTC+TGG	0.389287	2.1:-7402848	None:intergenic
AGAAATTCAACCAGAGGAAA+TGG	0.417568	2.1:+7402808	MS.gene052736:CDS
GAGATCCGACGGAGAGAGTT+GGG	0.442215	2.1:+7402742	MS.gene052736:intron
GCAAATGTTGCTTCCACCTC+AGG	0.445655	2.1:+7402982	MS.gene052736:CDS
AAGTTCGATGGTGCGGACTT+TGG	0.452497	2.1:+7402226	MS.gene052736:CDS
TGAACTTTATTTGATGCTGA+AGG	0.474628	2.1:-7402472	None:intergenic
TTTACCACGTCCATTTCCTC+TGG	0.487326	2.1:-7402818	None:intergenic
TGAGGCGGTTGTTTACGCTT+CGG	0.506448	2.1:+7402500	MS.gene052736:CDS
CGCGTCACCTCCTCCTGAGG+TGG	0.515832	2.1:-7402995	None:intergenic
GTGTTGGAACTGTGGAAAGA+TGG	0.515954	2.1:+7402903	MS.gene052736:CDS
AACCACCGCTGGACTTATGA+AGG	0.535609	2.1:+7402426	MS.gene052736:CDS
AAAGAAACCAGAATCGATGA+AGG	0.539324	2.1:+7402312	MS.gene052736:CDS
AGAGATCCGACGGAGAGAGT+TGG	0.549236	2.1:+7402741	MS.gene052736:intron
CAAATAAAGTTCATTTGATG+AGG	0.550433	2.1:+7402482	MS.gene052736:CDS
TTTACGCTTCGGATGACAGA+AGG	0.562705	2.1:+7402511	MS.gene052736:CDS
GTGAAAATTGAAAAGTTCGA+TGG	0.563926	2.1:+7402214	MS.gene052736:CDS
CTCTGTATGCAATACTGAAG+AGG	0.567853	2.1:-7402776	None:intergenic
ACAACCCAATTAAGTTCAGT+TGG	0.568124	2.1:+7402574	MS.gene052736:CDS
GTTGCTTCCACCTCAGGAGG+AGG	0.568689	2.1:+7402988	MS.gene052736:CDS
ACTCGAAATCTATCGAGTGT+TGG	0.576612	2.1:+7402887	MS.gene052736:CDS
TGTTGGAACTGTGGAAAGAT+GGG	0.586945	2.1:+7402904	MS.gene052736:CDS
AAATGGACGTGGTAAATCAA+AGG	0.596335	2.1:+7402825	MS.gene052736:CDS
TTCTCAGTGAAGAGATCCGA+CGG	0.596721	2.1:+7402731	MS.gene052736:intron
GATGACAGAAGGCGCGTCAG+TGG	0.598117	2.1:+7402522	MS.gene052736:CDS
ATAAAGTTCATTTGATGAGG+CGG	0.598437	2.1:+7402485	MS.gene052736:CDS
CTTCTCGATAGACAAGCACT+TGG	0.601850	2.1:+7402349	MS.gene052736:CDS
TCTATCGAGTGTTGGAACTG+TGG	0.601883	2.1:+7402895	MS.gene052736:CDS
AATGTTGCTTCCACCTCAGG+AGG	0.603928	2.1:+7402985	MS.gene052736:CDS
AATCGATGAAGGATGATGAG+TGG	0.606445	2.1:+7402323	MS.gene052736:CDS
GCAAAAGAGAAAACCACCGC+TGG	0.607234	2.1:+7402415	MS.gene052736:CDS
AATTGAAAAGTTCGATGGTG+CGG	0.608192	2.1:+7402219	MS.gene052736:CDS
CAAGTGCTTGTCTATCGAGA+AGG	0.612004	2.1:-7402348	None:intergenic
TATGGCTGCAGACGAAGGAA+AGG	0.614378	2.1:+7402192	None:intergenic
ATTCACCCAACTCTCTCCGT+CGG	0.616565	2.1:-7402747	None:intergenic
GGAGTTATGGCTGCAGACGA+AGG	0.617375	2.1:+7402187	None:intergenic
AGCCTTCATAAGTCCAGCGG+TGG	0.623238	2.1:-7402428	None:intergenic
GAATCAAGAGGCGAAAGATG+AGG	0.623736	2.1:+7402960	MS.gene052736:CDS
CAACGCGTCACCTCCTCCTG+AGG	0.630580	2.1:-7402998	None:intergenic
TGGTATCGAATTTGACGATG+AGG	0.639552	2.1:+7402594	MS.gene052736:CDS
AGAGGAAGAAATTCAACCAG+AGG	0.643795	2.1:+7402802	MS.gene052736:CDS
TCAACCAGAGGAAATGGACG+TGG	0.665050	2.1:+7402814	MS.gene052736:CDS
GCTCCCAGCAAAGAATCAAG+AGG	0.671318	2.1:+7402948	MS.gene052736:CDS
GTATTGCATACAGAGTCAAG+AGG	0.682691	2.1:+7402784	MS.gene052736:CDS
AAGAGCCTTCATAAGTCCAG+CGG	0.707222	2.1:-7402431	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAAATAAAGTTCATTTGATG+AGG	+	chr2.1:7402482-7402501	MS.gene052736:CDS	25.0%
	ATAAAGTTCATTTGATGAGG+CGG	+	chr2.1:7402485-7402504	MS.gene052736:CDS	30.0%
	GATACCAACTGAACTTAATT+GGG	-	chr2.1:7402581-7402600	None:intergenic	30.0%
	GTGAAAATTGAAAAGTTCGA+TGG	+	chr2.1:7402214-7402233	MS.gene052736:CDS	30.0%
!	TGAACTTTATTTGATGCTGA+AGG	-	chr2.1:7402475-7402494	None:intergenic	30.0%
	AAAGAAACCAGAATCGATGA+AGG	+	chr2.1:7402312-7402331	MS.gene052736:CDS	35.0%
	AAATGGACGTGGTAAATCAA+AGG	+	chr2.1:7402825-7402844	MS.gene052736:CDS	35.0%
	AATTGAAAAGTTCGATGGTG+CGG	+	chr2.1:7402219-7402238	MS.gene052736:CDS	35.0%
	ACAACCCAATTAAGTTCAGT+TGG	+	chr2.1:7402574-7402593	MS.gene052736:CDS	35.0%
	AGAAATTCAACCAGAGGAAA+TGG	+	chr2.1:7402808-7402827	MS.gene052736:CDS	35.0%
	CGATACCAACTGAACTTAAT+TGG	-	chr2.1:7402582-7402601	None:intergenic	35.0%
	GTCACTTCAAAAATCAGTGT+AGG	+	chr2.1:7402926-7402945	MS.gene052736:CDS	35.0%
!	ACTACGATGATTTCTGGATT+TGG	-	chr2.1:7402860-7402879	None:intergenic	35.0%
!!	ATTTGATGATGTTCGTGATC+TGG	+	chr2.1:7402708-7402727	MS.gene052736:intron	35.0%
	AATCGATGAAGGATGATGAG+TGG	+	chr2.1:7402323-7402342	MS.gene052736:CDS	40.0%
	ACTCGAAATCTATCGAGTGT+TGG	+	chr2.1:7402887-7402906	MS.gene052736:CDS	40.0%
	AGAGGAAGAAATTCAACCAG+AGG	+	chr2.1:7402802-7402821	MS.gene052736:CDS	40.0%
	CTCTGTATGCAATACTGAAG+AGG	-	chr2.1:7402779-7402798	None:intergenic	40.0%
	GTATTGCATACAGAGTCAAG+AGG	+	chr2.1:7402784-7402803	MS.gene052736:CDS	40.0%
	GTGAGAACTACGATGATTTC+TGG	-	chr2.1:7402866-7402885	None:intergenic	40.0%
	TGTTGGAACTGTGGAAAGAT+GGG	+	chr2.1:7402904-7402923	MS.gene052736:CDS	40.0%
!	ATTTCTGGATTTGGATCGTC+TGG	-	chr2.1:7402851-7402870	None:intergenic	40.0%
!	TGGTATCGAATTTGACGATG+AGG	+	chr2.1:7402594-7402613	MS.gene052736:CDS	40.0%
!!	TCATCATCCTTCATCGATTC+TGG	-	chr2.1:7402322-7402341	None:intergenic	40.0%
!!	TCTGGTTTCTTTTCTGTGAG+AGG	-	chr2.1:7402304-7402323	None:intergenic	40.0%
	AAGAGCCTTCATAAGTCCAG+CGG	-	chr2.1:7402434-7402453	None:intergenic	45.0%
	CAAGTGCTTGTCTATCGAGA+AGG	-	chr2.1:7402351-7402370	None:intergenic	45.0%
	CTTCTCGATAGACAAGCACT+TGG	+	chr2.1:7402349-7402368	MS.gene052736:CDS	45.0%
	GAATCAAGAGGCGAAAGATG+AGG	+	chr2.1:7402960-7402979	MS.gene052736:CDS	45.0%
	GTGTTGGAACTGTGGAAAGA+TGG	+	chr2.1:7402903-7402922	MS.gene052736:CDS	45.0%
	TCGCCTCTTGATTCTTTGCT+GGG	-	chr2.1:7402954-7402973	None:intergenic	45.0%
	TGTCTTCCCTACCAGATAGT+TGG	+	chr2.1:7402635-7402654	MS.gene052736:intron	45.0%
	TTCGCCTCTTGATTCTTTGC+TGG	-	chr2.1:7402955-7402974	None:intergenic	45.0%
	TTCTCAGTGAAGAGATCCGA+CGG	+	chr2.1:7402731-7402750	MS.gene052736:intron	45.0%
	TTTACCACGTCCATTTCCTC+TGG	-	chr2.1:7402821-7402840	None:intergenic	45.0%
	TTTACGCTTCGGATGACAGA+AGG	+	chr2.1:7402511-7402530	MS.gene052736:CDS	45.0%
!!	TCTATCGAGTGTTGGAACTG+TGG	+	chr2.1:7402895-7402914	MS.gene052736:CDS	45.0%
	AACCACCGCTGGACTTATGA+AGG	+	chr2.1:7402426-7402445	MS.gene052736:CDS	50.0%
	AAGTTCGATGGTGCGGACTT+TGG	+	chr2.1:7402226-7402245	MS.gene052736:CDS	50.0%
	ACAGTAGCGCTCCAACTATC+TGG	-	chr2.1:7402649-7402668	None:intergenic	50.0%
	AGCGCTCCAACTATCTGGTA+GGG	-	chr2.1:7402644-7402663	None:intergenic	50.0%
	ATTCACCCAACTCTCTCCGT+CGG	-	chr2.1:7402750-7402769	None:intergenic	50.0%
	GCAAAAGAGAAAACCACCGC+TGG	+	chr2.1:7402415-7402434	MS.gene052736:CDS	50.0%
	GCTCCCAGCAAAGAATCAAG+AGG	+	chr2.1:7402948-7402967	MS.gene052736:CDS	50.0%
	TAGCGCTCCAACTATCTGGT+AGG	-	chr2.1:7402645-7402664	None:intergenic	50.0%
	TCAACCAGAGGAAATGGACG+TGG	+	chr2.1:7402814-7402833	MS.gene052736:CDS	50.0%
	TGAGGCGGTTGTTTACGCTT+CGG	+	chr2.1:7402500-7402519	MS.gene052736:CDS	50.0%
!	AATGTTGCTTCCACCTCAGG+AGG	+	chr2.1:7402985-7403004	MS.gene052736:CDS	50.0%
!	GCAAATGTTGCTTCCACCTC+AGG	+	chr2.1:7402982-7403001	MS.gene052736:CDS	50.0%
!	TAGTTGGAGCGCTACTGTCA+CGG	+	chr2.1:7402651-7402670	MS.gene052736:intron	50.0%
!!!	ATGGTGCGGACTTTGGCTTT+TGG	+	chr2.1:7402233-7402252	MS.gene052736:CDS	50.0%
	ACGGCTGTGAGTAGCTCATC+GGG	+	chr2.1:7402670-7402689	MS.gene052736:intron	55.0%
	AGAGATCCGACGGAGAGAGT+TGG	+	chr2.1:7402741-7402760	MS.gene052736:intron	55.0%
	AGCCTTCATAAGTCCAGCGG+TGG	-	chr2.1:7402431-7402450	None:intergenic	55.0%
	CACGGCTGTGAGTAGCTCAT+CGG	+	chr2.1:7402669-7402688	MS.gene052736:intron	55.0%
	GAGATCCGACGGAGAGAGTT+GGG	+	chr2.1:7402742-7402761	MS.gene052736:intron	55.0%
	GTTGCTTCCACCTCAGGAGG+AGG	+	chr2.1:7402988-7403007	MS.gene052736:CDS	60.0%
!	GATGACAGAAGGCGCGTCAG+TGG	+	chr2.1:7402522-7402541	MS.gene052736:CDS	60.0%
	CAACGCGTCACCTCCTCCTG+AGG	-	chr2.1:7403001-7403020	None:intergenic	65.0%
	CGCGTCACCTCCTCCTGAGG+TGG	-	chr2.1:7402998-7403017	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	7402193	7403021	7402193	ID=MS.gene052736
chr2.1	mRNA	7402193	7403021	7402193	ID=MS.gene052736.t1;Parent=MS.gene052736
chr2.1	exon	7402193	7402615	7402193	ID=MS.gene052736.t1.exon1;Parent=MS.gene052736.t1
chr2.1	CDS	7402193	7402615	7402193	ID=cds.MS.gene052736.t1;Parent=MS.gene052736.t1
chr2.1	exon	7402743	7403021	7402743	ID=MS.gene052736.t1.exon2;Parent=MS.gene052736.t1
chr2.1	CDS	7402743	7403021	7402743	ID=cds.MS.gene052736.t1;Parent=MS.gene052736.t1

Gene Sequence

>MS.gene052736

ATGGCTGCAGACGAAGGAAAGGTGAAAATTGAAAAGTTCGATGGTGCGGACTTTGGCTTTTGGAAGATGCAGATCGAAGATTATTTGTATCAGAAAAAGCTACATCAACCTCTCACAGAAAAGAAACCAGAATCGATGAAGGATGATGAGTGGAACCTTCTCGATAGACAAGCACTTGGTGTTGTGCGATTGTCGTTATCTCGAAATGTTGCTTTTAACATAGCAAAAGAGAAAACCACCGCTGGACTTATGAAGGCTCTTTCTAGCATGTATGAAAAGCCTTCAGCATCAAATAAAGTTCATTTGATGAGGCGGTTGTTTACGCTTCGGATGACAGAAGGCGCGTCAGTGGCGCAACATATCAATGAACTTAATATTGTCACAACCCAATTAAGTTCAGTTGGTATCGAATTTGACGATGAGGTACGAGCATTGATACTTTTGTCTTCCCTACCAGATAGTTGGAGCGCTACTGTCACGGCTGTGAGTAGCTCATCGGGAAGCAAAAAGATGAAATTTGATGATGTTCGTGATCTGGTTCTCAGTGAAGAGATCCGACGGAGAGAGTTGGGTGAATCATCATCCTCTTCAGTATTGCATACAGAGTCAAGAGGAAGAAATTCAACCAGAGGAAATGGACGTGGTAAATCAAAGGCCAGACGATCCAAATCCAGAAATCATCGTAGTTCTCACAACTCGAAATCTATCGAGTGTTGGAACTGTGGAAAGATGGGTCACTTCAAAAATCAGTGTAGGCTCCCAGCAAAGAATCAAGAGGCGAAAGATGAGGCAAATGTTGCTTCCACCTCAGGAGGAGGTGACGCGTTGA

Protein sequence

>MS.gene052736.t1

MAADEGKVKIEKFDGADFGFWKMQIEDYLYQKKLHQPLTEKKPESMKDDEWNLLDRQALGVVRLSLSRNVAFNIAKEKTTAGLMKALSSMYEKPSASNKVHLMRRLFTLRMTEGASVAQHINELNIVTTQLSSVGIEFDDERSDGESWVNHHPLQYCIQSQEEEIQPEEMDVVNQRPDDPNPEIIVVLTTRNLSSVGTVERWVTSKISVGSQQRIKRRKMRQMLLPPQEEVTR