AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene95339


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95339.t1 MTR_2g039140 40.217 184 77 2 1 183 120 271 8.75e-35 124
MS.gene95339.t1 MTR_4g036430 89.744 39 4 0 1 39 31 69 1.55e-20 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene95339.t1 AT3G29785 57.778 90 37 1 11 100 7 95 1.04e-32 114

Find 38 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATACCAATTGAACTTAAT+TGG 0.076810 1.3:+18032414 None:intergenic
TCATCATCCTTCATCGATTC+TGG 0.247368 1.3:+18032674 None:intergenic
ATGGTGCGGACTTTGGCTTT+TGG 0.377376 1.3:-18032760 MS.gene95339:CDS
GATACCAATTGAACTTAATT+GGG 0.389115 1.3:+18032415 None:intergenic
ACAACCCAATTAAGTTCAAT+TGG 0.412295 1.3:-18032419 MS.gene95339:CDS
ACAGTAGCACTCCAACTATC+TGG 0.413891 1.3:+18032347 None:intergenic
TGTCTTCCCTACCAGATAGT+TGG 0.424640 1.3:-18032358 MS.gene95339:CDS
ATTTGACGATGTTCGTGATC+TGG 0.433721 1.3:-18032285 MS.gene95339:CDS
GAGATCCGACGGAGAGAGTT+GGG 0.442215 1.3:-18032251 MS.gene95339:CDS
AAGTTCGATGGTGCGGACTT+TGG 0.452497 1.3:-18032767 MS.gene95339:CDS
CACAGCTGTGAGTGGCTCCT+CGG 0.454182 1.3:-18032324 MS.gene95339:CDS
ACAGCTGTGAGTGGCTCCTC+GGG 0.470989 1.3:-18032323 MS.gene95339:CDS
TGAACTTTATTTGATGCTGA+AGG 0.474628 1.3:+18032521 None:intergenic
TGAGGCGGTTGTTTACGCTT+CGG 0.506448 1.3:-18032493 MS.gene95339:CDS
CTTTGTATGCAATACTGAAG+AGG 0.534584 1.3:+18032217 None:intergenic
AAAGAAACCAGAATCGATGA+AGG 0.539324 1.3:-18032681 MS.gene95339:CDS
AGAGATCCGACGGAGAGAGT+TGG 0.549236 1.3:-18032252 MS.gene95339:CDS
CAAATAAAGTTCATTTGATG+AGG 0.550433 1.3:-18032511 MS.gene95339:CDS
AACCACCGCTGGTCTTATGA+AGG 0.557775 1.3:-18032567 MS.gene95339:CDS
TAGCACTCCAACTATCTGGT+AGG 0.558134 1.3:+18032351 None:intergenic
TTTACGCTTCGGATGACAGA+AGG 0.562705 1.3:-18032482 MS.gene95339:CDS
GTGAAAATTGAAAAGTTCGA+TGG 0.563926 1.3:-18032779 MS.gene95339:CDS
GATGACAGAAGGCGCATCAG+TGG 0.593273 1.3:-18032471 MS.gene95339:CDS
GCTACTGTCACAGCTGTGAG+TGG 0.594785 1.3:-18032332 MS.gene95339:CDS
TTCTCAGTGAAGAGATCCGA+CGG 0.596721 1.3:-18032262 MS.gene95339:CDS
AGCACTCCAACTATCTGGTA+GGG 0.597976 1.3:+18032352 None:intergenic
ATAAAGTTCATTTGATGAGG+CGG 0.598437 1.3:-18032508 MS.gene95339:CDS
CTTCTCGATAGACAAGCACT+TGG 0.601850 1.3:-18032644 MS.gene95339:CDS
AATCGATGAAGGATGATGAG+TGG 0.606445 1.3:-18032670 MS.gene95339:CDS
AATTGAAAAGTTCGATGGTG+CGG 0.608192 1.3:-18032774 MS.gene95339:CDS
CAAGTGCTTGTCTATCGAGA+AGG 0.612004 1.3:+18032645 None:intergenic
TATGGCTGCAGACGAAGGAA+AGG 0.614378 1.3:-18032801 MS.gene95339:CDS
GGAGTTATGGCTGCAGACGA+AGG 0.617375 1.3:-18032806 None:intergenic
ATTCACCCAACTCTCTCCGT+CGG 0.618585 1.3:+18032246 None:intergenic
AGCCTTCATAAGACCAGCGG+TGG 0.620835 1.3:+18032565 None:intergenic
GCAAAAGAGAAAACCACCGC+TGG 0.626545 1.3:-18032578 MS.gene95339:CDS
TGGTATCGAATTTGACGATG+AGG 0.629176 1.3:-18032399 MS.gene95339:CDS
AAGAGCCTTCATAAGACCAG+CGG 0.707222 1.3:+18032562 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAAATAAAGTTCATTTGATG+AGG - chr1.3:18032503-18032522 MS.gene95339:CDS 25.0%
! GATACCAATTGAACTTAATT+GGG + chr1.3:18032602-18032621 None:intergenic 25.0%
ACAACCCAATTAAGTTCAAT+TGG - chr1.3:18032595-18032614 MS.gene95339:CDS 30.0%
ATAAAGTTCATTTGATGAGG+CGG - chr1.3:18032506-18032525 MS.gene95339:CDS 30.0%
CGATACCAATTGAACTTAAT+TGG + chr1.3:18032603-18032622 None:intergenic 30.0%
GTGAAAATTGAAAAGTTCGA+TGG - chr1.3:18032235-18032254 MS.gene95339:CDS 30.0%
! TGAACTTTATTTGATGCTGA+AGG + chr1.3:18032496-18032515 None:intergenic 30.0%
AAAGAAACCAGAATCGATGA+AGG - chr1.3:18032333-18032352 MS.gene95339:CDS 35.0%
AATTGAAAAGTTCGATGGTG+CGG - chr1.3:18032240-18032259 MS.gene95339:CDS 35.0%
CTTTGTATGCAATACTGAAG+AGG + chr1.3:18032800-18032819 None:intergenic 35.0%
AATCGATGAAGGATGATGAG+TGG - chr1.3:18032344-18032363 MS.gene95339:CDS 40.0%
ATTTGACGATGTTCGTGATC+TGG - chr1.3:18032729-18032748 MS.gene95339:CDS 40.0%
! TGGTATCGAATTTGACGATG+AGG - chr1.3:18032615-18032634 MS.gene95339:CDS 40.0%
! TTTCATCTTTTTGCTTCCCG+AGG + chr1.3:18032710-18032729 None:intergenic 40.0%
!! TCATCATCCTTCATCGATTC+TGG + chr1.3:18032343-18032362 None:intergenic 40.0%
!! TCTGGTTTCTTTTCTGTGAG+AGG + chr1.3:18032325-18032344 None:intergenic 40.0%
AAGAGCCTTCATAAGACCAG+CGG + chr1.3:18032455-18032474 None:intergenic 45.0%
ACAGTAGCACTCCAACTATC+TGG + chr1.3:18032670-18032689 None:intergenic 45.0%
AGCACTCCAACTATCTGGTA+GGG + chr1.3:18032665-18032684 None:intergenic 45.0%
CAAGTGCTTGTCTATCGAGA+AGG + chr1.3:18032372-18032391 None:intergenic 45.0%
CTTCTCGATAGACAAGCACT+TGG - chr1.3:18032370-18032389 MS.gene95339:CDS 45.0%
TAGCACTCCAACTATCTGGT+AGG + chr1.3:18032666-18032685 None:intergenic 45.0%
TGTCTTCCCTACCAGATAGT+TGG - chr1.3:18032656-18032675 MS.gene95339:CDS 45.0%
TTCTCAGTGAAGAGATCCGA+CGG - chr1.3:18032752-18032771 MS.gene95339:CDS 45.0%
TTTACGCTTCGGATGACAGA+AGG - chr1.3:18032532-18032551 MS.gene95339:CDS 45.0%
AAGTTCGATGGTGCGGACTT+TGG - chr1.3:18032247-18032266 MS.gene95339:CDS 50.0%
ATTCACCCAACTCTCTCCGT+CGG + chr1.3:18032771-18032790 None:intergenic 50.0%
GCAAAAGAGAAAACCACCGC+TGG - chr1.3:18032436-18032455 MS.gene95339:CDS 50.0%
TGAGGCGGTTGTTTACGCTT+CGG - chr1.3:18032521-18032540 MS.gene95339:CDS 50.0%
!! AACCACCGCTGGTCTTATGA+AGG - chr1.3:18032447-18032466 MS.gene95339:CDS 50.0%
!!! ATGGTGCGGACTTTGGCTTT+TGG - chr1.3:18032254-18032273 MS.gene95339:CDS 50.0%
AGAGATCCGACGGAGAGAGT+TGG - chr1.3:18032762-18032781 MS.gene95339:CDS 55.0%
AGCCTTCATAAGACCAGCGG+TGG + chr1.3:18032452-18032471 None:intergenic 55.0%
GAGATCCGACGGAGAGAGTT+GGG - chr1.3:18032763-18032782 MS.gene95339:CDS 55.0%
GCTACTGTCACAGCTGTGAG+TGG - chr1.3:18032682-18032701 MS.gene95339:CDS 55.0%
! GATGACAGAAGGCGCATCAG+TGG - chr1.3:18032543-18032562 MS.gene95339:CDS 55.0%
ACAGCTGTGAGTGGCTCCTC+GGG - chr1.3:18032691-18032710 MS.gene95339:CDS 60.0%
CACAGCTGTGAGTGGCTCCT+CGG - chr1.3:18032690-18032709 MS.gene95339:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 18032214 18032822 18032214 ID=MS.gene95339
chr1.3 mRNA 18032214 18032822 18032214 ID=MS.gene95339.t1;Parent=MS.gene95339
chr1.3 exon 18032214 18032822 18032214 ID=MS.gene95339.t1.exon1;Parent=MS.gene95339.t1
chr1.3 CDS 18032214 18032822 18032214 ID=cds.MS.gene95339.t1;Parent=MS.gene95339.t1
Gene Sequence

>MS.gene95339

ATGGCTGCAGACGAAGGAAAGGTGAAAATTGAAAAGTTCGATGGTGCGGACTTTGGCTTTTGGAAGATGCAGATCGAAGATTATTTGTATCAGAAAAAGCTACATCAACCTCTCACAGAAAAGAAACCAGAATCGATGAAGGATGATGAGTGGAACCTTCTCGATAGACAAGCACTTGGTGTTGTGCGATTGTCGTTATCTCGAAATGTTGCTTTTAACATAGCAAAAGAGAAAACCACCGCTGGTCTTATGAAGGCTCTTTCTAGCATGTACGAAAAGCCTTCAGCATCAAATAAAGTTCATTTGATGAGGCGGTTGTTTACGCTTCGGATGACAGAAGGCGCATCAGTGGCGCAACATATCAATGAACTTAATATTGTCACAACCCAATTAAGTTCAATTGGTATCGAATTTGACGATGAGGTACGAGCATTGATACTTTTGTCTTCCCTACCAGATAGTTGGAGTGCTACTGTCACAGCTGTGAGTGGCTCCTCGGGAAGCAAAAAGATGAAATTTGACGATGTTCGTGATCTGGTTCTCAGTGAAGAGATCCGACGGAGAGAGTTGGGTGAATCTTCATCCTCTTCAGTATTGCATACAAAGTAA

Protein sequence

>MS.gene95339.t1

MAADEGKVKIEKFDGADFGFWKMQIEDYLYQKKLHQPLTEKKPESMKDDEWNLLDRQALGVVRLSLSRNVAFNIAKEKTTAGLMKALSSMYEKPSASNKVHLMRRLFTLRMTEGASVAQHINELNIVTTQLSSIGIEFDDEVRALILLSSLPDSWSATVTAVSGSSGSKKMKFDDVRDLVLSEEIRRRELGESSSSSVLHTK