AlfalfaGEDB | MS.gene81140

M. sativa cultivar XinJiangDaYe / MS.gene81140

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81140.t1	XP_024640148.1	62.8	113	42	0	8	120	59	171	5.50E-34	153.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81140.t1	G7K4G4	62.8	113	42	0	8	120	709	821	4.0e-34	153.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene81140.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049587	MS.gene81140	0.80114	1.04E-48	-1.69E-46
MS.gene049853	MS.gene81140	0.817214	3.70E-52	-1.69E-46
MS.gene051932	MS.gene81140	0.862412	5.16E-64	-1.69E-46
MS.gene052447	MS.gene81140	0.805021	1.63E-49	-1.69E-46
MS.gene053491	MS.gene81140	0.813417	2.59E-51	-1.69E-46
MS.gene054174	MS.gene81140	0.843744	1.16E-58	-1.69E-46
MS.gene054175	MS.gene81140	0.850934	1.23E-60	-1.69E-46
MS.gene05435	MS.gene81140	0.825855	3.73E-54	-1.69E-46
MS.gene054484	MS.gene81140	0.817053	4.03E-52	-1.69E-46
MS.gene058515	MS.gene81140	0.809421	1.91E-50	-1.69E-46
MS.gene059458	MS.gene81140	0.826493	2.63E-54	-1.69E-46
MS.gene059459	MS.gene81140	0.808499	3.01E-50	-1.69E-46
MS.gene059460	MS.gene81140	0.808084	3.68E-50	-1.69E-46
MS.gene06044	MS.gene81140	0.835227	1.90E-56	-1.69E-46
MS.gene061245	MS.gene81140	0.835005	2.16E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81140.t1	MTR_5g094740	62.832	113	42	0	8	120	709	821	9.01e-44	152
MS.gene81140.t1	MTR_1g019580	54.464	112	51	0	8	119	19	130	1.22e-40	131
MS.gene81140.t1	MTR_1g105780	53.571	112	52	0	8	119	162	273	1.24e-39	133
MS.gene81140.t1	MTR_1g105765	53.571	112	52	0	8	119	162	273	1.30e-39	133
MS.gene81140.t1	MTR_3g452180	49.580	119	60	0	2	120	71	189	3.13e-37	125
MS.gene81140.t1	MTR_7g110090	49.580	119	60	0	2	120	155	273	6.66e-37	126
MS.gene81140.t1	MTR_4g032580	48.718	117	60	0	3	119	185	301	8.56e-36	124
MS.gene81140.t1	MTR_4g032560	48.718	117	60	0	3	119	185	301	8.93e-36	124
MS.gene81140.t1	MTR_4g062370	60.215	93	37	0	3	95	160	252	1.32e-31	117
MS.gene81140.t1	MTR_1g069260	46.341	123	54	1	8	118	166	288	3.70e-31	112
MS.gene81140.t1	MTR_8g042540	54.762	84	38	0	37	120	5	88	7.03e-30	103
MS.gene81140.t1	MTR_8g042540	54.762	84	38	0	37	120	17	100	8.40e-30	103
MS.gene81140.t1	MTR_1g047540	45.299	117	64	0	2	118	140	256	2.36e-28	107
MS.gene81140.t1	MTR_1g087870	40.541	111	66	0	9	119	115	225	5.25e-28	103
MS.gene81140.t1	MTR_4g121040	45.536	112	61	0	2	113	59	170	3.18e-27	99.8
MS.gene81140.t1	MTR_0152s0060	47.191	89	47	0	24	112	228	316	2.38e-22	90.5
MS.gene81140.t1	MTR_3g048970	40.404	99	59	0	20	118	178	276	1.27e-21	87.0
MS.gene81140.t1	MTR_1g084880	40.404	99	59	0	20	118	178	276	1.33e-21	87.0
MS.gene81140.t1	MTR_3g070250	36.792	106	67	0	13	118	176	281	4.90e-20	82.8
MS.gene81140.t1	MTR_3g085990	41.379	116	49	3	4	119	140	236	2.26e-19	80.1
MS.gene81140.t1	MTR_4g057880	43.662	71	40	0	48	118	2	72	9.33e-18	72.0
MS.gene81140.t1	MTR_2g016390	40.230	87	52	0	11	97	602	688	3.68e-17	76.3
MS.gene81140.t1	MTR_4g081330	50.000	62	31	0	38	99	1085	1146	9.75e-15	69.3
MS.gene81140.t1	MTR_2g100360	36.170	94	59	1	4	97	163	255	9.51e-14	65.9
MS.gene81140.t1	MTR_6g016445	50.000	60	30	0	42	101	13	72	1.95e-13	62.0
MS.gene81140.t1	MTR_4g117740	49.123	57	29	0	6	62	141	197	3.00e-13	63.5
MS.gene81140.t1	MTR_1g037600	39.326	89	53	1	13	100	71	159	3.62e-13	64.3
MS.gene81140.t1	MTR_2g072800	43.284	67	38	0	39	105	202	268	4.11e-12	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 29 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGATGAATGTGGTTGTATA+TGG	0.268575	6.1:-54144884	MS.gene81140:CDS
ATGCAGGATTTGGCTCATAT+TGG	0.281266	6.1:-54144954	MS.gene81140:intron
GACTTTGTAGAGATCGATTC+TGG	0.282449	6.1:-54145063	MS.gene81140:CDS
TGCAGGATTTGGCTCATATT+GGG	0.361232	6.1:-54144953	MS.gene81140:intron
GGTCAAGGCTAGAAGGTTAA+AGG	0.364042	6.1:-54144793	MS.gene81140:CDS
TTCTCATTGACACCGATATA+TGG	0.377396	6.1:+54144744	None:intergenic
GACGGTGACAGTGTCGTTAT+CGG	0.398058	6.1:-54144771	MS.gene81140:CDS
TGCACAAATGGCACAGGAAA+AGG	0.399613	6.1:+54145126	None:intergenic
GTTATCGGTGCTCCATATAT+CGG	0.401878	6.1:-54144756	MS.gene81140:CDS
CCTCATGCTCACTTTAAGAT+TGG	0.409855	6.1:-54144834	MS.gene81140:CDS
AGATTGGCGGTGGCTGGAAC+AGG	0.416190	6.1:-54144818	MS.gene81140:CDS
ACTTTAAGATTGGCGGTGGC+TGG	0.418939	6.1:-54144824	MS.gene81140:CDS
TGAAACTGAGAATCTGAGTT+TGG	0.418987	6.1:-54144907	MS.gene81140:CDS
TTAAATTCATTATGCACAAA+TGG	0.431684	6.1:+54145114	None:intergenic
AGAGATCGATTCTGGAATCT+TGG	0.440229	6.1:-54145055	MS.gene81140:intron
CATGAGGGCGTGTAACAAAC+TGG	0.441451	6.1:+54144850	None:intergenic
ACAGGATGGTCAAGGCTAGA+AGG	0.443921	6.1:-54144800	MS.gene81140:CDS
CATGCTCACTTTAAGATTGG+CGG	0.482279	6.1:-54144831	MS.gene81140:CDS
CTGAGTTTGGTTGATGAATG+TGG	0.483514	6.1:-54144894	MS.gene81140:CDS
TGATGAATGTGGTTGTATAT+GGG	0.484155	6.1:-54144883	MS.gene81140:CDS
GCTCACTTTAAGATTGGCGG+TGG	0.568885	6.1:-54144828	MS.gene81140:CDS
CCAATCTTAAAGTGAGCATG+AGG	0.578221	6.1:+54144834	None:intergenic
TCATTATGCACAAATGGCAC+AGG	0.601819	6.1:+54145120	None:intergenic
TGGCGGTGGCTGGAACAGGA+TGG	0.649155	6.1:-54144814	MS.gene81140:CDS
TGGCTGGAACAGGATGGTCA+AGG	0.676417	6.1:-54144808	MS.gene81140:CDS
AGGGCGTGTAACAAACTGGA+GGG	0.676724	6.1:+54144854	None:intergenic
CAATCTTAAAGTGAGCATGA+GGG	0.680650	6.1:+54144835	None:intergenic
AAGGCTAGAAGGTTAAAGGA+CGG	0.693112	6.1:-54144789	MS.gene81140:CDS
GAGGGCGTGTAACAAACTGG+AGG	0.699235	6.1:+54144853	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAATTCATTATGCACAAA+TGG	+	chr6.1:54144746-54144765	None:intergenic	20.0%
!!!	ATATGATGTTTTAATTATGC+AGG	-	chr6.1:54144887-54144906	MS.gene81140:CDS	20.0%
!!!	TGTTAAAAAAGATATTGACT+TGG	-	chr6.1:54144836-54144855	MS.gene81140:CDS	20.0%
!!!	TGTTTTAATTATGCAGGATT+TGG	-	chr6.1:54144893-54144912	MS.gene81140:CDS	25.0%
	TGATGAATGTGGTTGTATAT+GGG	-	chr6.1:54144974-54144993	MS.gene81140:intron	30.0%
	TTGATGAATGTGGTTGTATA+TGG	-	chr6.1:54144973-54144992	MS.gene81140:intron	30.0%
	CAATCTTAAAGTGAGCATGA+GGG	+	chr6.1:54145025-54145044	None:intergenic	35.0%
	TGAAACTGAGAATCTGAGTT+TGG	-	chr6.1:54144950-54144969	MS.gene81140:CDS	35.0%
	TTCTCATTGACACCGATATA+TGG	+	chr6.1:54145116-54145135	None:intergenic	35.0%
	AAGGCTAGAAGGTTAAAGGA+CGG	-	chr6.1:54145068-54145087	MS.gene81140:CDS	40.0%
	CCAATCTTAAAGTGAGCATG+AGG	+	chr6.1:54145026-54145045	None:intergenic	40.0%
	TCATTATGCACAAATGGCAC+AGG	+	chr6.1:54144740-54144759	None:intergenic	40.0%
!	ATGCAGGATTTGGCTCATAT+TGG	-	chr6.1:54144903-54144922	MS.gene81140:CDS	40.0%
!	GACTTTGTAGAGATCGATTC+TGG	-	chr6.1:54144794-54144813	MS.gene81140:CDS	40.0%
!	GTTATCGGTGCTCCATATAT+CGG	-	chr6.1:54145101-54145120	MS.gene81140:CDS	40.0%
!	TGCAGGATTTGGCTCATATT+GGG	-	chr6.1:54144904-54144923	MS.gene81140:CDS	40.0%
!!	AGAGATCGATTCTGGAATCT+TGG	-	chr6.1:54144802-54144821	MS.gene81140:CDS	40.0%
!!	CATGCTCACTTTAAGATTGG+CGG	-	chr6.1:54145026-54145045	MS.gene81140:intron	40.0%
!!	CCTCATGCTCACTTTAAGAT+TGG	-	chr6.1:54145023-54145042	MS.gene81140:intron	40.0%
!!	CTGAGTTTGGTTGATGAATG+TGG	-	chr6.1:54144963-54144982	MS.gene81140:intron	40.0%
	GGTCAAGGCTAGAAGGTTAA+AGG	-	chr6.1:54145064-54145083	MS.gene81140:CDS	45.0%
	TGCACAAATGGCACAGGAAA+AGG	+	chr6.1:54144734-54144753	None:intergenic	45.0%
	ACAGGATGGTCAAGGCTAGA+AGG	-	chr6.1:54145057-54145076	MS.gene81140:CDS	50.0%
	AGGGCGTGTAACAAACTGGA+GGG	+	chr6.1:54145006-54145025	None:intergenic	50.0%
!	CATGAGGGCGTGTAACAAAC+TGG	+	chr6.1:54145010-54145029	None:intergenic	50.0%
!	GACGGTGACAGTGTCGTTAT+CGG	-	chr6.1:54145086-54145105	MS.gene81140:CDS	50.0%
!!	ACTTTAAGATTGGCGGTGGC+TGG	-	chr6.1:54145033-54145052	MS.gene81140:intron	50.0%
!!	GCTCACTTTAAGATTGGCGG+TGG	-	chr6.1:54145029-54145048	MS.gene81140:intron	50.0%
	TGGCTGGAACAGGATGGTCA+AGG	-	chr6.1:54145049-54145068	MS.gene81140:intron	55.0%
!	GAGGGCGTGTAACAAACTGG+AGG	+	chr6.1:54145007-54145026	None:intergenic	55.0%
	AGATTGGCGGTGGCTGGAAC+AGG	-	chr6.1:54145039-54145058	MS.gene81140:intron	60.0%
	TGGCGGTGGCTGGAACAGGA+TGG	-	chr6.1:54145043-54145062	MS.gene81140:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	54144716	54145163	54144716	ID=MS.gene81140
chr6.1	mRNA	54144716	54145163	54144716	ID=MS.gene81140.t1;Parent=MS.gene81140
chr6.1	exon	54145056	54145163	54145056	ID=MS.gene81140.t1.exon1;Parent=MS.gene81140.t1
chr6.1	CDS	54145056	54145163	54145056	ID=cds.MS.gene81140.t1;Parent=MS.gene81140.t1
chr6.1	exon	54144716	54144970	54144716	ID=MS.gene81140.t1.exon2;Parent=MS.gene81140.t1
chr6.1	CDS	54144716	54144970	54144716	ID=cds.MS.gene81140.t1;Parent=MS.gene81140.t1

Gene Sequence

>MS.gene81140

ATGCATTTGTTCAATCCTTTTCCTGTGCCATTTGTGCATAATGAATTTAACTTTCAACTGACATATGAGAAAAAGCTTGACTTTGTAGAGATCGATTCTGGAATCTTGGTATGTTTTATGTGTTAAAAAAGATATTGACTTGGTGTTGCAATTGATTCGACTTCAACTAATATATGATGTTTTAATTATGCAGGATTTGGCTCATATTGGGTTCTGTCAGCTTGCTTTGAACACTGAAACTGAGAATCTGAGTTTGGTTGATGAATGTGGTTGTATATGGGATTGCACCCTCCAGTTTGTTACACGCCCTCATGCTCACTTTAAGATTGGCGGTGGCTGGAACAGGATGGTCAAGGCTAGAAGGTTAAAGGACGGTGACAGTGTCGTTATCGGTGCTCCATATATCGGTGTCAATGAGAAGCTCTTTTTTTTTGTTGTTCGTTATTAG

Protein sequence

>MS.gene81140.t1

MHLFNPFPVPFVHNEFNFQLTYEKKLDFVEIDSGILDLAHIGFCQLALNTETENLSLVDECGCIWDCTLQFVTRPHAHFKIGGGWNRMVKARRLKDGDSVVIGAPYIGVNEKLFFFVVRY