AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054429

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054429.t1 MTR_7g112580 99.265 544 4 0 1 544 1 544 0.0 1132
MS.gene054429.t1 MTR_4g087980 47.581 496 223 8 68 542 37 516 8.84e-159 463
MS.gene054429.t1 MTR_2g044880 47.699 478 222 8 80 542 61 525 2.63e-158 462
MS.gene054429.t1 MTR_3g091380 45.920 527 256 10 40 543 78 598 8.77e-156 458
MS.gene054429.t1 MTR_7g108905 41.187 556 304 8 1 543 12 557 2.81e-150 442
MS.gene054429.t1 MTR_1g008140 48.319 476 208 9 99 543 140 608 2.75e-149 442
MS.gene054429.t1 MTR_4g087970 43.912 501 246 10 64 543 32 518 1.49e-143 424
MS.gene054429.t1 MTR_4g025730 49.666 449 188 10 102 542 91 509 6.32e-143 422
MS.gene054429.t1 MTR_4g025690 49.339 454 189 10 100 542 90 513 2.64e-138 410
MS.gene054429.t1 MTR_8g042900 41.571 522 282 7 34 542 70 581 1.14e-136 409
MS.gene054429.t1 MTR_8g104655 45.070 497 235 10 68 542 61 541 5.02e-136 405
MS.gene054429.t1 MTR_3g010780 43.121 487 240 6 73 542 108 574 1.77e-135 405
MS.gene054429.t1 MTR_1g083520 44.304 474 230 8 89 542 69 528 5.37e-135 402
MS.gene054429.t1 MTR_7g050980 45.275 455 218 8 102 542 118 555 4.47e-133 398
MS.gene054429.t1 MTR_8g023310 44.111 467 234 6 98 542 128 589 5.31e-133 399
MS.gene054429.t1 MTR_7g050870 41.651 521 261 13 44 542 55 554 3.12e-131 394
MS.gene054429.t1 MTR_1g054880 44.731 465 227 8 106 542 96 558 7.65e-130 390
MS.gene054429.t1 MTR_2g044810 42.017 476 249 8 76 542 77 534 1.40e-129 389
MS.gene054429.t1 MTR_4g025670 43.827 486 224 11 78 542 32 489 5.03e-129 385
MS.gene054429.t1 MTR_1g088020 44.325 467 228 8 106 542 91 555 6.43e-128 385
MS.gene054429.t1 MTR_8g104645 53.846 338 143 3 218 542 35 372 3.97e-127 377
MS.gene054429.t1 MTR_7g050950 42.035 521 258 16 44 542 55 553 9.25e-126 379
MS.gene054429.t1 MTR_4g124520 43.590 429 217 6 127 542 105 521 2.98e-125 377
MS.gene054429.t1 MTR_3g010770 42.213 488 241 7 95 544 93 577 5.12e-125 379
MS.gene054429.t1 MTR_8g042910 43.182 484 230 10 98 542 135 612 3.72e-124 377
MS.gene054429.t1 MTR_7g108875 44.421 466 228 9 106 542 101 564 9.36e-124 375
MS.gene054429.t1 MTR_1g088225 43.683 467 229 9 106 542 91 553 5.85e-122 370
MS.gene054429.t1 MTR_7g053360 44.270 445 218 8 109 542 102 527 2.60e-121 367
MS.gene054429.t1 MTR_3g008640 47.433 409 189 7 145 542 134 527 5.53e-121 366
MS.gene054429.t1 MTR_8g023150 42.209 507 272 8 44 538 81 578 8.00e-121 368
MS.gene054429.t1 MTR_3g008720 47.188 409 190 7 145 542 134 527 5.68e-120 364
MS.gene054429.t1 MTR_3g033690 47.073 410 189 8 145 542 133 526 7.37e-120 363
MS.gene054429.t1 MTR_1g086190 38.230 531 287 13 40 543 48 564 1.53e-119 364
MS.gene054429.t1 MTR_4g035980 43.860 456 230 9 99 542 98 539 2.08e-119 363
MS.gene054429.t1 MTR_3g008700 46.472 411 194 7 143 542 132 527 2.31e-119 362
MS.gene054429.t1 MTR_7g108890 44.037 436 198 9 144 542 136 562 1.31e-117 359
MS.gene054429.t1 MTR_1g083490 35.888 535 317 10 23 538 44 571 4.70e-114 350
MS.gene054429.t1 MTR_1g083470 34.177 553 341 10 3 538 20 566 4.83e-114 350
MS.gene054429.t1 MTR_1g083500 34.177 553 341 10 3 538 20 566 4.83e-114 350
MS.gene054429.t1 MTR_1g083510 35.271 499 292 7 72 541 86 582 6.98e-114 350
MS.gene054429.t1 MTR_7g108900 41.614 471 238 11 106 543 95 561 1.13e-113 349
MS.gene054429.t1 MTR_1g086210 36.961 533 294 11 44 542 45 569 1.75e-113 348
MS.gene054429.t1 MTR_7g112590 37.302 504 284 9 69 544 86 585 1.03e-112 347
MS.gene054429.t1 MTR_4g005180 36.719 512 300 9 44 538 49 553 3.09e-112 345
MS.gene054429.t1 MTR_4g005170 36.914 512 299 9 44 538 67 571 3.66e-112 347
MS.gene054429.t1 MTR_4g124550 38.618 492 275 10 69 542 111 593 3.50e-110 341
MS.gene054429.t1 MTR_0458s0030 56.786 280 107 3 272 542 40 314 1.44e-109 330
MS.gene054429.t1 MTR_1g088040 56.786 280 107 3 272 542 40 314 1.44e-109 330
MS.gene054429.t1 MTR_2g044660 36.796 568 286 13 39 544 78 634 8.54e-109 338
MS.gene054429.t1 MTR_8g104580 40.040 497 227 12 68 542 53 500 2.45e-107 330
MS.gene054429.t1 MTR_2g436040 34.429 517 298 10 44 539 47 543 1.40e-105 329
MS.gene054429.t1 MTR_7g110560 34.035 570 319 16 3 542 7 549 3.02e-104 324
MS.gene054429.t1 MTR_4g120280 33.966 527 315 10 37 541 56 571 3.49e-102 320
MS.gene054429.t1 MTR_4g120290 32.836 536 331 8 26 541 45 571 2.47e-101 317
MS.gene054429.t1 MTR_7g110440 33.945 545 318 10 30 542 32 566 1.07e-98 310
MS.gene054429.t1 MTR_2g436050 33.397 521 321 9 37 541 53 563 1.11e-98 310
MS.gene054429.t1 MTR_3g095920 33.391 578 313 13 1 541 21 563 3.72e-97 306
MS.gene054429.t1 MTR_7g110490 35.110 544 315 11 30 543 32 567 1.32e-96 305
MS.gene054429.t1 MTR_7g110510 35.110 544 315 11 30 543 32 567 1.32e-96 305
MS.gene054429.t1 MTR_8g093460 33.979 568 321 13 7 544 10 553 8.37e-95 300
MS.gene054429.t1 MTR_2g044810 52.590 251 109 2 301 542 216 465 1.37e-92 291
MS.gene054429.t1 MTR_0618s0020 54.185 227 95 1 325 542 2 228 2.81e-85 264
MS.gene054429.t1 MTR_7g110420 33.880 549 311 14 30 537 32 569 5.90e-82 267
MS.gene054429.t1 MTR_7g110430 32.402 537 303 10 30 542 32 532 1.75e-81 265
MS.gene054429.t1 MTR_8g088610 40.774 336 178 7 218 539 45 373 2.09e-80 256
MS.gene054429.t1 MTR_2g105560 41.639 305 156 4 247 542 48 339 2.88e-75 242
MS.gene054429.t1 MTR_5g083620 36.873 339 194 6 219 542 23 356 8.10e-74 239
MS.gene054429.t1 MTR_0458s0010 34.423 459 248 11 106 542 91 518 1.87e-72 240
MS.gene054429.t1 MTR_7g107180 41.158 311 162 5 242 542 8 307 2.28e-71 231
MS.gene054429.t1 MTR_7g111560 32.692 520 304 15 43 542 63 556 3.99e-70 235
MS.gene054429.t1 MTR_6g024165 36.778 329 178 8 216 533 27 336 2.71e-58 197
MS.gene054429.t1 MTR_6g088810 33.333 381 227 11 172 531 3 377 4.82e-57 195
MS.gene054429.t1 MTR_1g086540 36.213 301 172 6 243 531 52 344 1.18e-56 193
MS.gene054429.t1 MTR_6g012800 37.413 286 159 5 243 515 8 286 2.21e-55 189
MS.gene054429.t1 MTR_2g039530 40.594 303 157 10 217 509 30 319 5.26e-55 189
MS.gene054429.t1 MTR_8g102380 35.473 296 172 5 249 531 88 377 1.77e-52 183
MS.gene054429.t1 MTR_7g011530 34.545 275 162 3 243 504 8 277 1.33e-48 171
MS.gene054429.t1 MTR_7g102600 33.446 296 187 4 243 531 101 393 1.98e-48 173
MS.gene054429.t1 MTR_7g092780 32.542 295 186 5 247 531 96 387 3.25e-47 169
MS.gene054429.t1 MTR_6g042660 34.601 263 158 5 247 504 43 296 4.68e-47 167
MS.gene054429.t1 MTR_5g093970 33.677 291 187 4 242 531 44 329 4.63e-46 165
MS.gene054429.t1 MTR_5g012600 32.915 319 192 7 232 534 50 362 1.93e-45 164
MS.gene054429.t1 MTR_6g042690 34.432 273 160 5 242 504 40 303 2.67e-45 162
MS.gene054429.t1 MTR_8g102380 36.667 240 136 3 249 475 88 324 3.84e-44 159
MS.gene054429.t1 MTR_5g095490 32.323 297 183 5 247 531 67 357 1.39e-43 159
MS.gene054429.t1 MTR_1g033050 33.684 285 173 6 232 504 4 284 4.86e-43 156
MS.gene054429.t1 MTR_4g124740 32.890 301 173 9 243 531 56 339 1.40e-41 153
MS.gene054429.t1 MTR_2g033970 29.097 299 194 6 243 532 58 347 4.15e-41 152
MS.gene054429.t1 MTR_5g083590 29.206 315 163 6 238 542 4 268 3.34e-40 147
MS.gene054429.t1 MTR_5g088300 31.633 294 182 5 242 531 38 316 1.08e-36 139
MS.gene054429.t1 MTR_2g436600 27.703 296 193 8 243 532 57 337 2.51e-36 138
MS.gene054429.t1 MTR_4g074290 29.618 314 203 7 226 531 58 361 4.51e-35 135
MS.gene054429.t1 MTR_2g073390 28.889 315 204 8 226 531 58 361 2.79e-34 133
MS.gene054429.t1 MTR_8g036225 30.147 272 172 5 243 504 35 298 1.02e-32 128
MS.gene054429.t1 MTR_5g088290 30.556 288 190 3 242 526 49 329 2.05e-32 127
MS.gene054429.t1 MTR_4g051810 29.866 298 197 5 236 531 30 317 6.21e-32 125
MS.gene054429.t1 MTR_5g088280 29.965 287 190 4 242 525 40 318 3.62e-31 124
MS.gene054429.t1 MTR_0009s0350 28.014 282 192 5 226 501 58 334 4.17e-31 124
MS.gene054429.t1 MTR_0009s0340 28.014 282 192 5 226 501 58 334 4.17e-31 124
MS.gene054429.t1 MTR_2g436070 26.349 315 218 7 2 312 17 321 1.18e-30 123
MS.gene054429.t1 MTR_6g025590 30.653 199 119 4 210 400 29 216 6.92e-27 109
MS.gene054429.t1 MTR_0408s0030 43.182 88 50 0 417 504 36 123 5.49e-21 90.1
MS.gene054429.t1 MTR_6g024200 33.537 164 96 4 369 531 125 276 3.83e-18 85.1
MS.gene054429.t1 MTR_7g108790 30.128 156 106 3 40 194 34 187 3.03e-17 80.5
MS.gene054429.t1 MTR_4g035870 39.216 102 53 3 99 195 96 193 6.76e-15 73.9
MS.gene054429.t1 MTR_4g035880 39.604 101 52 3 99 194 96 192 6.89e-15 73.9
MS.gene054429.t1 MTR_7g102580 45.349 86 37 2 315 395 73 153 1.31e-14 72.0
MS.gene054429.t1 MTR_4g130790 27.854 219 137 8 264 473 48 254 2.83e-14 73.6
MS.gene054429.t1 MTR_4g035905 38.182 110 55 4 94 194 87 192 2.16e-13 69.3
MS.gene054429.t1 MTR_1g088540 29.921 127 87 2 69 194 15 140 4.69e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054429.t1 AT3G05620 62.157 547 186 6 9 544 7 543 0.0 699
MS.gene054429.t1 AT4G33220 50.612 490 213 6 79 542 37 523 8.51e-171 494
MS.gene054429.t1 AT3G43270 49.582 478 228 5 76 542 50 525 3.74e-167 484
MS.gene054429.t1 AT4G33220 53.086 405 166 4 159 542 1 402 9.26e-152 441
MS.gene054429.t1 AT5G53370 46.217 489 238 7 72 543 106 586 6.75e-151 445
MS.gene054429.t1 AT5G53370 46.217 489 238 7 72 543 142 622 2.50e-150 445
MS.gene054429.t1 AT3G49220 43.954 521 265 6 40 543 87 597 1.57e-147 437
MS.gene054429.t1 AT3G49220 43.954 521 265 6 40 543 132 642 8.02e-147 437
MS.gene054429.t1 AT2G45220 43.063 555 253 16 2 542 6 511 3.60e-137 407
MS.gene054429.t1 AT1G23200 47.346 471 211 8 98 542 95 554 1.34e-136 407
MS.gene054429.t1 AT1G11580 44.420 448 226 5 102 538 118 553 4.00e-132 396
MS.gene054429.t1 AT4G02300 41.767 498 258 8 62 542 50 532 5.15e-132 395
MS.gene054429.t1 AT1G11580 44.420 448 226 5 102 538 139 574 9.13e-132 396
MS.gene054429.t1 AT3G05610 39.394 528 275 11 40 542 58 565 4.32e-131 397
MS.gene054429.t1 AT5G27870 37.838 518 295 6 40 542 57 562 1.02e-129 395
MS.gene054429.t1 AT2G26440 41.085 516 278 9 44 542 41 547 1.58e-128 386
MS.gene054429.t1 AT1G53830 44.731 465 231 6 102 542 125 587 2.09e-128 387
MS.gene054429.t1 AT2G43050 43.813 493 241 10 63 544 51 518 7.72e-128 384
MS.gene054429.t1 AT4G02320 40.400 500 250 7 73 542 37 518 1.46e-127 383
MS.gene054429.t1 AT3G10710 43.802 484 244 10 68 542 97 561 2.16e-127 384
MS.gene054429.t1 AT3G14310 42.283 473 239 5 102 542 122 592 4.92e-126 382
MS.gene054429.t1 AT3G60730 41.988 493 247 7 78 542 38 519 1.15e-125 378
MS.gene054429.t1 AT5G51490 43.035 481 244 10 77 542 71 536 4.87e-124 375
MS.gene054429.t1 AT4G00190 44.843 446 202 8 106 542 64 474 3.95e-123 370
MS.gene054429.t1 AT4G02330 42.259 478 233 9 106 542 96 571 3.91e-122 371
MS.gene054429.t1 AT3G47400 39.227 543 290 10 24 542 68 594 1.71e-120 367
MS.gene054429.t1 AT3G59010 43.607 438 234 6 107 542 102 528 3.32e-119 362
MS.gene054429.t1 AT5G04960 40.787 483 261 7 68 540 95 562 3.36e-118 360
MS.gene054429.t1 AT5G51500 41.164 481 253 10 77 542 75 540 1.05e-117 358
MS.gene054429.t1 AT5G04970 43.396 477 226 12 103 542 151 620 9.70e-117 358
MS.gene054429.t1 AT4G33230 38.302 530 300 12 31 542 86 606 1.96e-116 357
MS.gene054429.t1 AT1G02810 40.562 498 223 8 106 542 92 577 1.02e-115 355
MS.gene054429.t1 AT3G10720 41.632 478 243 11 98 542 141 615 1.48e-115 355
MS.gene054429.t1 AT1G53840 39.010 505 280 11 52 536 85 581 5.25e-115 353
MS.gene054429.t1 AT5G49180 36.932 528 306 11 31 538 46 566 1.13e-114 352
MS.gene054429.t1 AT2G47550 44.304 474 213 14 106 542 99 558 1.38e-114 351
MS.gene054429.t1 AT2G26450 40.246 487 266 12 68 542 138 611 2.14e-114 352
MS.gene054429.t1 AT5G20860 40.133 451 232 10 108 542 110 538 2.57e-105 327
MS.gene054429.t1 AT4G03930 42.446 417 209 8 140 542 125 524 1.02e-104 324
MS.gene054429.t1 AT3G06830 35.472 530 299 12 40 540 51 566 1.21e-104 325
MS.gene054429.t1 AT1G11590 42.271 414 214 6 140 542 125 524 1.51e-102 319
MS.gene054429.t1 AT3G10720 55.769 260 105 3 292 542 1 259 4.89e-102 308
MS.gene054429.t1 AT3G14300 36.821 497 292 8 52 533 471 960 6.22e-102 329
MS.gene054429.t1 AT3G27980 40.494 405 217 5 147 542 108 497 3.71e-98 306
MS.gene054429.t1 AT4G15980 44.380 347 180 4 206 540 355 700 5.34e-92 296
MS.gene054429.t1 AT4G15980 44.380 347 180 4 206 540 365 710 9.47e-92 296
MS.gene054429.t1 AT5G09760 36.090 532 277 17 36 530 41 546 5.85e-89 284
MS.gene054429.t1 AT5G09760 35.888 535 280 17 33 530 64 572 1.04e-88 285
MS.gene054429.t1 AT5G64640 33.272 547 279 15 38 534 91 601 4.80e-86 278
MS.gene054429.t1 AT5G20860 36.009 436 213 9 108 527 110 495 1.51e-80 261
MS.gene054429.t1 AT3G62170 29.982 557 326 14 37 542 45 588 5.96e-76 251
MS.gene054429.t1 AT2G47030 29.983 597 348 15 3 542 5 588 1.32e-75 250
MS.gene054429.t1 AT1G11370 44.487 263 132 2 219 472 29 286 1.94e-72 233
MS.gene054429.t1 AT2G47040 31.041 509 280 12 95 542 97 595 9.11e-72 240
MS.gene054429.t1 AT2G36710 34.884 301 186 4 243 536 93 390 3.46e-55 191
MS.gene054429.t1 AT5G19730 33.333 354 203 8 202 531 37 381 7.27e-54 187
MS.gene054429.t1 AT2G36700 32.153 339 207 6 198 531 13 333 1.18e-52 182
MS.gene054429.t1 AT1G05310 33.333 300 187 5 242 531 92 388 1.21e-49 176
MS.gene054429.t1 AT3G29090 34.615 312 179 7 243 537 9 312 3.25e-49 172
MS.gene054429.t1 AT3G17060 31.544 298 190 5 242 532 47 337 1.33e-48 172
MS.gene054429.t1 AT1G44980 44.149 188 103 1 219 404 54 241 5.04e-46 162
MS.gene054429.t1 AT5G47500 29.428 367 226 8 183 533 11 360 8.08e-46 165
MS.gene054429.t1 AT2G21610 31.615 291 186 5 243 531 53 332 8.51e-45 162
MS.gene054429.t1 AT2G21610 31.615 291 186 5 243 531 58 337 1.61e-44 161
MS.gene054429.t1 AT5G07430 34.021 291 178 7 247 531 73 355 7.52e-43 157
MS.gene054429.t1 AT5G18990 32.595 316 189 7 228 531 20 323 3.91e-41 151
MS.gene054429.t1 AT3G24130 30.938 320 194 9 228 531 20 328 1.15e-40 150
MS.gene054429.t1 AT5G55590 31.788 302 188 7 242 531 83 378 1.47e-38 145
MS.gene054429.t1 AT5G55590 32.927 246 150 5 242 475 83 325 2.94e-38 143
MS.gene054429.t1 AT3G49220 30.345 290 184 5 40 321 132 411 1.54e-37 144
MS.gene054429.t1 AT5G61680 29.912 341 202 9 204 531 42 358 1.32e-36 140
MS.gene054429.t1 AT2G19150 31.081 296 189 5 251 538 51 339 1.37e-36 139
MS.gene054429.t1 AT5G26810 32.258 279 168 5 242 504 35 308 3.61e-36 138
MS.gene054429.t1 AT5G07420 31.081 296 190 7 242 531 68 355 1.21e-33 131
MS.gene054429.t1 AT1G69940 27.891 294 189 7 249 531 74 355 7.40e-33 129
MS.gene054429.t1 AT2G19150 32.618 233 145 4 251 475 51 279 9.89e-33 127
MS.gene054429.t1 AT5G07410 27.551 294 190 7 249 531 74 355 1.30e-32 129
MS.gene054429.t1 AT2G47280 28.808 302 195 7 243 533 37 329 1.65e-29 119
MS.gene054429.t1 AT3G62820 27.979 193 124 5 5 194 9 189 5.83e-14 71.2
MS.gene054429.t1 AT5G62360 32.593 135 85 3 58 190 64 194 7.55e-13 68.2
MS.gene054429.t1 AT1G62770 28.000 200 120 6 7 194 8 195 3.87e-11 63.2

Find 141 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCTCACAACCTACATATT+TGG 0.095781 7.4:-4144293 MS.gene054429:CDS
CAGACCAATCTGCATTCTTT+AGG 0.260515 7.4:-4144552 MS.gene054429:CDS
TGTTAATGTGTAGCTGTATC+TGG 0.260734 7.4:-4144658 MS.gene054429:intron
GCCTCACAACCTACATATTT+GGG 0.269773 7.4:-4144292 MS.gene054429:CDS
AGCTTAATCGCTTACCCTTT+AGG 0.279269 7.4:-4145185 MS.gene054429:CDS
AAGCTTGCATATCTTGAGTT+TGG 0.290313 7.4:+4145670 None:intergenic
AATCCAAGATAGTTATGTTT+TGG 0.298560 7.4:-4144314 MS.gene054429:CDS
CTTGATCAAATGATGGTAAA+AGG 0.301062 7.4:+4145727 None:intergenic
TGTTTGTGAGGCAGGAGTTT+TGG 0.320217 7.4:+4145616 None:intergenic
GTGAACTTAACACCTGTTCT+AGG 0.327842 7.4:+4144016 None:intergenic
TACGGTGAATACAGGAATTA+TGG 0.331114 7.4:-4144154 MS.gene054429:CDS
TGGACTTGGAGGGCCTGTTC+TGG 0.341206 7.4:+4145578 None:intergenic
CTACATATTTGGGGAGGCCT+TGG 0.347308 7.4:-4144282 MS.gene054429:CDS
TTCTTTAGGTGTAGCATAGA+AGG 0.349482 7.4:-4144538 MS.gene054429:CDS
GGTTCCTTAACGGCGGTTCA+TGG 0.359467 7.4:-4144042 MS.gene054429:CDS
TCAGAGCTTGCATGGTCGTT+AGG 0.365066 7.4:-4145408 MS.gene054429:CDS
GATCTTTGTCATGTTGTTGA+TGG 0.368569 7.4:+4145503 None:intergenic
GCCACGGCTTGATGGTTTAC+CGG 0.370851 7.4:+4144589 None:intergenic
TCAGCCTCTGTCATCCATTC+AGG 0.371531 7.4:+4145108 None:intergenic
GAGCTCCTTGCAATCTTCTA+TGG 0.371794 7.4:+4145446 None:intergenic
TACCGTGAATGCGAAATCTA+TGG 0.377605 7.4:-4144475 MS.gene054429:CDS
CACGCAATGCCACGGCTTGA+TGG 0.381995 7.4:+4144581 None:intergenic
AACTAGTACTGATGAAAGTT+TGG 0.382732 7.4:-4145136 MS.gene054429:CDS
ATGTCTGAGGGAATTTCATC+TGG 0.384523 7.4:+4145693 None:intergenic
TTACCGGTCCAGCAGTGTTC+CGG 0.387620 7.4:+4144605 None:intergenic
CAGAGCTTGCATGGTCGTTA+GGG 0.395199 7.4:-4145407 MS.gene054429:CDS
TCTACCAGCGAGCGATGAAC+CGG 0.402703 7.4:+4144129 None:intergenic
ACGGTTCAGCGGTTCCTTAA+CGG 0.416002 7.4:-4144052 MS.gene054429:CDS
GATGCAGTTGTTGCGTTGGA+TGG 0.417977 7.4:-4145045 MS.gene054429:CDS
AGGCCAAAACATAACTATCT+TGG 0.418609 7.4:+4144311 None:intergenic
CCGCACTTAGCCATGCTTCG+AGG 0.428133 7.4:+4145334 None:intergenic
CAGCTCCTAGTCATAGCAAT+AGG 0.443935 7.4:-4144984 MS.gene054429:CDS
CCGCTGAACCGTAAAATAAC+CGG 0.446817 7.4:+4144063 None:intergenic
TAACACATACATGAGTAGTA+TGG 0.446857 7.4:-4144233 MS.gene054429:CDS
ATGACATTCCGGAACACTGC+TGG 0.450390 7.4:-4144613 MS.gene054429:CDS
CATGTTGTTGATGGCACCTA+TGG 0.452823 7.4:+4145512 None:intergenic
ACAGAACATTACTGATAAGC+TGG 0.453691 7.4:+4145220 None:intergenic
GTTATGAATGTTTGTGAGGC+AGG 0.453911 7.4:+4145608 None:intergenic
ATTAAAGACGCTTCTGCCGC+CGG 0.455714 7.4:-4144082 MS.gene054429:CDS
TAACAGCTTCGTTGATTGTA+CGG 0.460625 7.4:+4145010 None:intergenic
CGTCTTGAGAGTTACATTAG+TGG 0.463916 7.4:-4145261 MS.gene054429:CDS
TCATGCAAGGTAGTGTTCCT+TGG 0.464611 7.4:+4145159 None:intergenic
GCAACCATGAACCGCCGTTA+AGG 0.465728 7.4:+4144038 None:intergenic
CATAACGAGCTAACCAGAAC+AGG 0.467307 7.4:-4145591 MS.gene054429:CDS
ACTTTGCATTAGACACTTTG+TGG 0.470978 7.4:-4144177 MS.gene054429:CDS
TTCCATAGATTTCGCATTCA+CGG 0.471515 7.4:+4144473 None:intergenic
GGTTCATGGTTGCCTAGAAC+AGG 0.472411 7.4:-4144028 MS.gene054429:CDS
TTGCGAGGGACATGACATTC+CGG 0.473496 7.4:-4144624 MS.gene054429:CDS
AGTTCCACTTCCATTACAAA+AGG 0.474475 7.4:-4144389 MS.gene054429:CDS
CTTTGTGGTACGGTGAATAC+AGG 0.485605 7.4:-4144162 MS.gene054429:CDS
ATTCCGGAACACTGCTGGAC+CGG 0.491888 7.4:-4144608 MS.gene054429:CDS
ACAGGTGTTAAGTTCACAGC+AGG 0.494156 7.4:-4144010 MS.gene054429:CDS
ACATGTATTGAAGGGTTTGA+AGG 0.494549 7.4:-4145294 MS.gene054429:CDS
CGCTCGCTGGTAGAGTCAAA+TGG 0.495741 7.4:-4144120 MS.gene054429:CDS
ACCCTTCAATACATGTGTCT+TGG 0.497187 7.4:+4145301 None:intergenic
ACCTATGGCTTCATTGATTG+TGG 0.498951 7.4:+4145527 None:intergenic
ACATGTTATCTATGTGAAGA+AGG 0.503880 7.4:-4144959 MS.gene054429:CDS
ATGACAGAAGTTGGACTTGG+AGG 0.503881 7.4:+4145567 None:intergenic
AACCAAGACACATGTATTGA+AGG 0.506538 7.4:-4145303 MS.gene054429:CDS
GCAGCATTAATGACAGAAGT+TGG 0.507875 7.4:+4145558 None:intergenic
AAAGTTTGGAGTTTCCTGAA+TGG 0.509972 7.4:-4145122 MS.gene054429:CDS
AACCTCGAGGGTGGCTTGAG+TGG 0.510238 7.4:-4144207 MS.gene054429:CDS
GTAAAAGGATAAGAATGAAA+AGG 0.512279 7.4:+4145742 None:intergenic
AGTGTTCCTTGGAGACCTAA+AGG 0.513393 7.4:+4145170 None:intergenic
GTTCTAGAATATTCCTTCCA+AGG 0.522274 7.4:+4144265 None:intergenic
ATGATCGTTGGTGATGGTAT+TGG 0.525137 7.4:-4144889 MS.gene054429:CDS
GTGTTCCTTGGAGACCTAAA+GGG 0.526257 7.4:+4145171 None:intergenic
TGACAGAAGTTGGACTTGGA+GGG 0.528123 7.4:+4145568 None:intergenic
TTAATGACAGAAGTTGGACT+TGG 0.530794 7.4:+4145564 None:intergenic
GTGAAACCTGTGCTTTGATG+TGG 0.535485 7.4:+4144337 None:intergenic
GCATTAGACACTTTGTGGTA+CGG 0.536809 7.4:-4144172 MS.gene054429:CDS
GAGAGAACTTGATCAAATGA+TGG 0.538479 7.4:+4145720 None:intergenic
TTTGATTGCACAAGCTTGCA+TGG 0.540945 7.4:-4145649 MS.gene054429:CDS
CGAGGGTGGCTTGAGTGGCT+CGG 0.541369 7.4:-4144202 MS.gene054429:CDS
GCTCTGACACAGAGAAATCA+AGG 0.541696 7.4:+4145424 None:intergenic
TCTCTGTGTCAGAGCTTGCA+TGG 0.541979 7.4:-4145416 MS.gene054429:CDS
ATGCAGTTGTTGCGTTGGAT+GGG 0.542294 7.4:-4145044 MS.gene054429:CDS
CAAGGCCTCCCCAAATATGT+AGG 0.548118 7.4:+4144283 None:intergenic
ATGTGTAGCTGTATCTGGGA+AGG 0.551122 7.4:-4144653 MS.gene054429:intron
TGGGAAGGGTTTCATTGCGA+GGG 0.552908 7.4:-4144638 MS.gene054429:CDS
ATTGCGATTGCTTGTAACAA+TGG 0.557427 7.4:+4144861 None:intergenic
GCTGGTAGAGTCAAATGGCC+TGG 0.558166 7.4:-4144115 MS.gene054429:CDS
ATATTTGGGGAGGCCTTGGA+AGG 0.559715 7.4:-4144278 MS.gene054429:CDS
TGTGTAGCTGTATCTGGGAA+GGG 0.561947 7.4:-4144652 MS.gene054429:intron
TGCAGATGCAGTTGTTGCGT+TGG 0.563751 7.4:-4145049 MS.gene054429:CDS
ATCAGTACTAGTTTCATGCA+AGG 0.565775 7.4:+4145146 None:intergenic
ACCAAGACACATGTATTGAA+GGG 0.567012 7.4:-4145302 MS.gene054429:CDS
AAAAGTCCACATCAAAGCAC+AGG 0.570765 7.4:-4144343 MS.gene054429:CDS
TACACCTAAAGAATGCAGAT+TGG 0.571861 7.4:+4144548 None:intergenic
CTGGGAAGGGTTTCATTGCG+AGG 0.574319 7.4:-4144639 MS.gene054429:CDS
ATCGCAATTTCATGCAAGGA+TGG 0.575589 7.4:-4144846 MS.gene054429:CDS
AAGGTTACAATCACAGCTCA+AGG 0.578151 7.4:-4144370 MS.gene054429:CDS
AACATCATGATCGTTGGTGA+TGG 0.582464 7.4:-4144895 MS.gene054429:CDS
AAACGTGGCAGTTCGAAAAG+TGG 0.582606 7.4:+4144822 None:intergenic
TGTGAAGAAGGGACTATACA+AGG 0.586315 7.4:-4144947 MS.gene054429:CDS
GTTAATGTGTAGCTGTATCT+GGG 0.587683 7.4:-4144657 MS.gene054429:intron
TCTTGAGTTTGGATGTCTGA+GGG 0.587826 7.4:+4145681 None:intergenic
GTTGTTGCGTTGGATGGGAG+CGG 0.587962 7.4:-4145039 MS.gene054429:CDS
AGCAATCGCAATTTCATGCA+AGG 0.589039 7.4:-4144850 MS.gene054429:CDS
CATGTTATCTATGTGAAGAA+GGG 0.590596 7.4:-4144958 MS.gene054429:CDS
GGACCCGGTTCATCGCTCGC+TGG 0.591181 7.4:-4144133 MS.gene054429:CDS
ATCACCAACGATCATGATGT+TGG 0.592216 7.4:+4144897 None:intergenic
AGTGTGCGTAAAGTGTGTCT+TGG 0.597316 7.4:+4144512 None:intergenic
ACGAAGGAAACCTCGAAGCA+TGG 0.597380 7.4:-4145344 MS.gene054429:CDS
ATGACCAACATCATGATCGT+TGG 0.598022 7.4:-4144901 MS.gene054429:CDS
ATCTTGAGTTTGGATGTCTG+AGG 0.599334 7.4:+4145680 None:intergenic
CAGAACATTACTGATAAGCT+GGG 0.605848 7.4:+4145221 None:intergenic
TTACAATCACAGCTCAAGGT+AGG 0.609803 7.4:-4144366 MS.gene054429:CDS
GAAAAGGAAATTCACAAGCA+AGG 0.615432 7.4:+4145758 None:intergenic
CTTCTATGGCAAGTTGCTCG+CGG 0.617529 7.4:+4145460 None:intergenic
GCTTACCCTTTAGGTCTCCA+AGG 0.618807 7.4:-4145176 MS.gene054429:CDS
CATGGTCGTTAGGGGAGATG+AGG 0.619966 7.4:-4145398 MS.gene054429:CDS
ACTTGCCATAGAAGATTGCA+AGG 0.620029 7.4:-4145451 MS.gene054429:CDS
CCCCAAATATGTAGGTTGTG+AGG 0.623360 7.4:+4144291 None:intergenic
GTAGTATGGTTCAACCTCGA+GGG 0.629470 7.4:-4144219 MS.gene054429:CDS
AGTAGTATGGTTCAACCTCG+AGG 0.630686 7.4:-4144220 MS.gene054429:CDS
TGAATCGACACGCAATGCCA+CGG 0.630967 7.4:+4144573 None:intergenic
GACAGAACTGCGCAGTACGA+AGG 0.633449 7.4:-4145360 MS.gene054429:CDS
GAGATGAGGAGAATTCGAGC+CGG 0.635377 7.4:-4145384 MS.gene054429:CDS
CTACCAGCGAGCGATGAACC+GGG 0.640025 7.4:+4144130 None:intergenic
TGTCTGAGGGAATTTCATCT+GGG 0.640715 7.4:+4145694 None:intergenic
GTACTGCGCAGTTCTGTCGC+CGG 0.644383 7.4:+4145365 None:intergenic
GCTCGTTATGAATGTTTGTG+AGG 0.644784 7.4:+4145604 None:intergenic
CCTCACAACCTACATATTTG+GGG 0.650108 7.4:-4144291 MS.gene054429:CDS
AGAGCTTGCATGGTCGTTAG+GGG 0.652304 7.4:-4145406 MS.gene054429:CDS
TGTCTCCTATTGCTATGACT+AGG 0.654035 7.4:+4144979 None:intergenic
GTATGGTTCAACCTCGAGGG+TGG 0.654064 7.4:-4144216 MS.gene054429:CDS
TCTTTAATGACATGATAACC+AGG 0.655480 7.4:+4144097 None:intergenic
CCTCGAAGCATGGCTAAGTG+CGG 0.658255 7.4:-4145334 MS.gene054429:CDS
ACCACAATCAATGAAGCCAT+AGG 0.663784 7.4:-4145528 MS.gene054429:CDS
ACCGGTAAACCATCAAGCCG+TGG 0.664465 7.4:-4144590 MS.gene054429:CDS
GAATACAGGAATTATGGACC+CGG 0.671183 7.4:-4144148 MS.gene054429:CDS
CACAACCTACATATTTGGGG+AGG 0.675764 7.4:-4144288 MS.gene054429:CDS
CTACTTAAATCTAAGCCGCA+CGG 0.678224 7.4:-4145078 MS.gene054429:CDS
AGAACTCTACTTACCAAACG+TGG 0.684668 7.4:+4144807 None:intergenic
AGCCACTCAAGCCACCCTCG+AGG 0.711855 7.4:+4144205 None:intergenic
GTTCAGCGGTTCCTTAACGG+CGG 0.714803 7.4:-4144049 MS.gene054429:CDS
CAGGTGTTAAGTTCACAGCA+GGG 0.718376 7.4:-4144009 MS.gene054429:CDS
CTGAACCGTAAAATAACCGG+CGG 0.728593 7.4:+4144066 None:intergenic
GTTTCCTGAATGGATGACAG+AGG 0.756653 7.4:-4145112 MS.gene054429:CDS
GCATCTGCAATCTTACCGTG+CGG 0.785544 7.4:+4145063 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTAAAAGGATAAGAATGAAA+AGG + chr7.4:4144020-4144039 None:intergenic 25.0%
! TGAATTGGTATATAGTGAAT+TGG - chr7.4:4145064-4145083 MS.gene054429:CDS 25.0%
!! AATCCAAGATAGTTATGTTT+TGG - chr7.4:4145445-4145464 MS.gene054429:CDS 25.0%
ACATGTTATCTATGTGAAGA+AGG - chr7.4:4144800-4144819 MS.gene054429:intron 30.0%
ATTCCCAAAAATGAAGTCTA+TGG + chr7.4:4145312-4145331 None:intergenic 30.0%
CATGTTATCTATGTGAAGAA+GGG - chr7.4:4144801-4144820 MS.gene054429:intron 30.0%
CTTGATCAAATGATGGTAAA+AGG + chr7.4:4144035-4144054 None:intergenic 30.0%
TAACACATACATGAGTAGTA+TGG - chr7.4:4145526-4145545 MS.gene054429:CDS 30.0%
TCTTTAATGACATGATAACC+AGG + chr7.4:4145665-4145684 None:intergenic 30.0%
! ACACACTCTTTTCAATATGT+AGG + chr7.4:4145037-4145056 None:intergenic 30.0%
! GTGATTGTAACCTTTTGTAA+TGG + chr7.4:4145383-4145402 None:intergenic 30.0%
!! AACTAGTACTGATGAAAGTT+TGG - chr7.4:4144623-4144642 MS.gene054429:CDS 30.0%
!! ATAGACTTCATTTTTGGGAA+TGG - chr7.4:4145311-4145330 MS.gene054429:CDS 30.0%
!! GAACCATAGACTTCATTTTT+GGG - chr7.4:4145306-4145325 MS.gene054429:CDS 30.0%
AACAGTATATCTCTAGCGTA+AGG - chr7.4:4144980-4144999 MS.gene054429:CDS 35.0%
AACCAAGACACATGTATTGA+AGG - chr7.4:4144456-4144475 MS.gene054429:CDS 35.0%
AAGCTTGCATATCTTGAGTT+TGG + chr7.4:4144092-4144111 None:intergenic 35.0%
ACAGAACATTACTGATAAGC+TGG + chr7.4:4144542-4144561 None:intergenic 35.0%
ACATGTATTGAAGGGTTTGA+AGG - chr7.4:4144465-4144484 MS.gene054429:CDS 35.0%
ACCAAGACACATGTATTGAA+GGG - chr7.4:4144457-4144476 MS.gene054429:CDS 35.0%
ACTTTGCATTAGACACTTTG+TGG - chr7.4:4145582-4145601 MS.gene054429:CDS 35.0%
AGGCCAAAACATAACTATCT+TGG + chr7.4:4145451-4145470 None:intergenic 35.0%
AGTTCCACTTCCATTACAAA+AGG - chr7.4:4145370-4145389 MS.gene054429:CDS 35.0%
ATTGCGATTGCTTGTAACAA+TGG + chr7.4:4144901-4144920 None:intergenic 35.0%
CAGAACATTACTGATAAGCT+GGG + chr7.4:4144541-4144560 None:intergenic 35.0%
GAAAAGGAAATTCACAAGCA+AGG + chr7.4:4144004-4144023 None:intergenic 35.0%
GAGAGAACTTGATCAAATGA+TGG + chr7.4:4144042-4144061 None:intergenic 35.0%
GAGTGTGTTAGAGAATGAAT+TGG - chr7.4:4145049-4145068 MS.gene054429:CDS 35.0%
GATCTTTGTCATGTTGTTGA+TGG + chr7.4:4144259-4144278 None:intergenic 35.0%
GTTAATGTGTAGCTGTATCT+GGG - chr7.4:4145102-4145121 MS.gene054429:CDS 35.0%
TACACCTAAAGAATGCAGAT+TGG + chr7.4:4145214-4145233 None:intergenic 35.0%
TACGGTGAATACAGGAATTA+TGG - chr7.4:4145605-4145624 MS.gene054429:CDS 35.0%
TGTTAATGTGTAGCTGTATC+TGG - chr7.4:4145101-4145120 MS.gene054429:CDS 35.0%
TTAATGACAGAAGTTGGACT+TGG + chr7.4:4144198-4144217 None:intergenic 35.0%
TTCCATAGATTTCGCATTCA+CGG + chr7.4:4145289-4145308 None:intergenic 35.0%
TTCTTTAGGTGTAGCATAGA+AGG - chr7.4:4145221-4145240 MS.gene054429:CDS 35.0%
! AAAGTTTGGAGTTTCCTGAA+TGG - chr7.4:4144637-4144656 MS.gene054429:CDS 35.0%
! ATCAGTACTAGTTTCATGCA+AGG + chr7.4:4144616-4144635 None:intergenic 35.0%
! GGAACCATAGACTTCATTTT+TGG - chr7.4:4145305-4145324 MS.gene054429:CDS 35.0%
! TAACAGCTTCGTTGATTGTA+CGG + chr7.4:4144752-4144771 None:intergenic 35.0%
!! GTAACCTTTTGTAATGGAAG+TGG + chr7.4:4145377-4145396 None:intergenic 35.0%
!! GTTCTAGAATATTCCTTCCA+AGG + chr7.4:4145497-4145516 None:intergenic 35.0%
AAAAGTCCACATCAAAGCAC+AGG - chr7.4:4145416-4145435 MS.gene054429:CDS 40.0%
AAGGTTACAATCACAGCTCA+AGG - chr7.4:4145389-4145408 MS.gene054429:CDS 40.0%
ACCACAATCAATGAAGCCAT+AGG - chr7.4:4144231-4144250 MS.gene054429:CDS 40.0%
ACCCTTCAATACATGTGTCT+TGG + chr7.4:4144461-4144480 None:intergenic 40.0%
ACTTGCCATAGAAGATTGCA+AGG - chr7.4:4144308-4144327 MS.gene054429:CDS 40.0%
AGAACTCTACTTACCAAACG+TGG + chr7.4:4144955-4144974 None:intergenic 40.0%
AGCAATCGCAATTTCATGCA+AGG - chr7.4:4144909-4144928 MS.gene054429:CDS 40.0%
AGCTTAATCGCTTACCCTTT+AGG - chr7.4:4144574-4144593 MS.gene054429:CDS 40.0%
ATCACCAACGATCATGATGT+TGG + chr7.4:4144865-4144884 None:intergenic 40.0%
ATCGCAATTTCATGCAAGGA+TGG - chr7.4:4144913-4144932 MS.gene054429:CDS 40.0%
ATCTTGAGTTTGGATGTCTG+AGG + chr7.4:4144082-4144101 None:intergenic 40.0%
ATGACCAACATCATGATCGT+TGG - chr7.4:4144858-4144877 MS.gene054429:CDS 40.0%
ATGTCTGAGGGAATTTCATC+TGG + chr7.4:4144069-4144088 None:intergenic 40.0%
CAGACCAATCTGCATTCTTT+AGG - chr7.4:4145207-4145226 MS.gene054429:CDS 40.0%
CCTCACAACCTACATATTTG+GGG - chr7.4:4145468-4145487 MS.gene054429:CDS 40.0%
CGTCTTGAGAGTTACATTAG+TGG - chr7.4:4144498-4144517 MS.gene054429:CDS 40.0%
CTACTTAAATCTAAGCCGCA+CGG - chr7.4:4144681-4144700 MS.gene054429:intron 40.0%
GAATACAGGAATTATGGACC+CGG - chr7.4:4145611-4145630 MS.gene054429:CDS 40.0%
GCAGCATTAATGACAGAAGT+TGG + chr7.4:4144204-4144223 None:intergenic 40.0%
GCATTAGACACTTTGTGGTA+CGG - chr7.4:4145587-4145606 MS.gene054429:CDS 40.0%
GCCTCACAACCTACATATTT+GGG - chr7.4:4145467-4145486 MS.gene054429:CDS 40.0%
GCTCGTTATGAATGTTTGTG+AGG + chr7.4:4144158-4144177 None:intergenic 40.0%
GTGAACTTAACACCTGTTCT+AGG + chr7.4:4145746-4145765 None:intergenic 40.0%
GTTATGAATGTTTGTGAGGC+AGG + chr7.4:4144154-4144173 None:intergenic 40.0%
TACCGTGAATGCGAAATCTA+TGG - chr7.4:4145284-4145303 MS.gene054429:CDS 40.0%
TCTTGAGTTTGGATGTCTGA+GGG + chr7.4:4144081-4144100 None:intergenic 40.0%
TGTCTCCTATTGCTATGACT+AGG + chr7.4:4144783-4144802 None:intergenic 40.0%
TGTCTGAGGGAATTTCATCT+GGG + chr7.4:4144068-4144087 None:intergenic 40.0%
TGTGAAGAAGGGACTATACA+AGG - chr7.4:4144812-4144831 MS.gene054429:intron 40.0%
TTACAATCACAGCTCAAGGT+AGG - chr7.4:4145393-4145412 MS.gene054429:CDS 40.0%
TTTGATTGCACAAGCTTGCA+TGG - chr7.4:4144110-4144129 MS.gene054429:CDS 40.0%
! AACATCATGATCGTTGGTGA+TGG - chr7.4:4144864-4144883 MS.gene054429:CDS 40.0%
! ACCTATGGCTTCATTGATTG+TGG + chr7.4:4144235-4144254 None:intergenic 40.0%
!! ATGATCGTTGGTGATGGTAT+TGG - chr7.4:4144870-4144889 MS.gene054429:CDS 40.0%
AAACGTGGCAGTTCGAAAAG+TGG + chr7.4:4144940-4144959 None:intergenic 45.0%
ACAGGTGTTAAGTTCACAGC+AGG - chr7.4:4145749-4145768 MS.gene054429:CDS 45.0%
AGTAGTATGGTTCAACCTCG+AGG - chr7.4:4145539-4145558 MS.gene054429:CDS 45.0%
AGTGTGCGTAAAGTGTGTCT+TGG + chr7.4:4145250-4145269 None:intergenic 45.0%
AGTGTTCCTTGGAGACCTAA+AGG + chr7.4:4144592-4144611 None:intergenic 45.0%
ATGACAGAAGTTGGACTTGG+AGG + chr7.4:4144195-4144214 None:intergenic 45.0%
ATGCAGTTGTTGCGTTGGAT+GGG - chr7.4:4144715-4144734 MS.gene054429:intron 45.0%
ATGTGTAGCTGTATCTGGGA+AGG - chr7.4:4145106-4145125 MS.gene054429:CDS 45.0%
CACAACCTACATATTTGGGG+AGG - chr7.4:4145471-4145490 MS.gene054429:CDS 45.0%
CAGCTCCTAGTCATAGCAAT+AGG - chr7.4:4144775-4144794 MS.gene054429:intron 45.0%
CAGGTGTTAAGTTCACAGCA+GGG - chr7.4:4145750-4145769 MS.gene054429:CDS 45.0%
CATAACGAGCTAACCAGAAC+AGG - chr7.4:4144168-4144187 MS.gene054429:CDS 45.0%
CCCCAAATATGTAGGTTGTG+AGG + chr7.4:4145471-4145490 None:intergenic 45.0%
CCGCTGAACCGTAAAATAAC+CGG + chr7.4:4145699-4145718 None:intergenic 45.0%
CTGAACCGTAAAATAACCGG+CGG + chr7.4:4145696-4145715 None:intergenic 45.0%
CTTTGTGGTACGGTGAATAC+AGG - chr7.4:4145597-4145616 MS.gene054429:CDS 45.0%
GAGCTCCTTGCAATCTTCTA+TGG + chr7.4:4144316-4144335 None:intergenic 45.0%
GCTCTGACACAGAGAAATCA+AGG + chr7.4:4144338-4144357 None:intergenic 45.0%
GGCCTCACAACCTACATATT+TGG - chr7.4:4145466-4145485 MS.gene054429:CDS 45.0%
GTAGTATGGTTCAACCTCGA+GGG - chr7.4:4145540-4145559 MS.gene054429:CDS 45.0%
GTGAAACCTGTGCTTTGATG+TGG + chr7.4:4145425-4145444 None:intergenic 45.0%
GTGTTCCTTGGAGACCTAAA+GGG + chr7.4:4144591-4144610 None:intergenic 45.0%
GTTTCCTGAATGGATGACAG+AGG - chr7.4:4144647-4144666 MS.gene054429:CDS 45.0%
TCATGCAAGGTAGTGTTCCT+TGG + chr7.4:4144603-4144622 None:intergenic 45.0%
TGACAGAAGTTGGACTTGGA+GGG + chr7.4:4144194-4144213 None:intergenic 45.0%
TGTGTAGCTGTATCTGGGAA+GGG - chr7.4:4145107-4145126 MS.gene054429:CDS 45.0%
! ACTTTTCGAACTGCCACGTT+TGG - chr7.4:4144939-4144958 MS.gene054429:CDS 45.0%
! TGTTTGTGAGGCAGGAGTTT+TGG + chr7.4:4144146-4144165 None:intergenic 45.0%
!! CATGTTGTTGATGGCACCTA+TGG + chr7.4:4144250-4144269 None:intergenic 45.0%
!! CCGGTTATTTTACGGTTCAG+CGG - chr7.4:4145696-4145715 MS.gene054429:CDS 45.0%
!! TTCTGCCGCCGGTTATTTTA+CGG - chr7.4:4145688-4145707 MS.gene054429:CDS 45.0%
ACGAAGGAAACCTCGAAGCA+TGG - chr7.4:4144415-4144434 MS.gene054429:CDS 50.0%
ACGGTTCAGCGGTTCCTTAA+CGG - chr7.4:4145707-4145726 MS.gene054429:CDS 50.0%
AGAGCTTGCATGGTCGTTAG+GGG - chr7.4:4144353-4144372 MS.gene054429:CDS 50.0%
ATATTTGGGGAGGCCTTGGA+AGG - chr7.4:4145481-4145500 MS.gene054429:CDS 50.0%
ATGACATTCCGGAACACTGC+TGG - chr7.4:4145146-4145165 MS.gene054429:CDS 50.0%
ATTAAAGACGCTTCTGCCGC+CGG - chr7.4:4145677-4145696 MS.gene054429:CDS 50.0%
CAAGGCCTCCCCAAATATGT+AGG + chr7.4:4145479-4145498 None:intergenic 50.0%
CAGAGCTTGCATGGTCGTTA+GGG - chr7.4:4144352-4144371 MS.gene054429:CDS 50.0%
CTACATATTTGGGGAGGCCT+TGG - chr7.4:4145477-4145496 MS.gene054429:CDS 50.0%
CTTCTATGGCAAGTTGCTCG+CGG + chr7.4:4144302-4144321 None:intergenic 50.0%
GAGATGAGGAGAATTCGAGC+CGG - chr7.4:4144375-4144394 MS.gene054429:CDS 50.0%
GATGCAGTTGTTGCGTTGGA+TGG - chr7.4:4144714-4144733 MS.gene054429:intron 50.0%
GCATCTGCAATCTTACCGTG+CGG + chr7.4:4144699-4144718 None:intergenic 50.0%
GCTTACCCTTTAGGTCTCCA+AGG - chr7.4:4144583-4144602 MS.gene054429:CDS 50.0%
GGTTCATGGTTGCCTAGAAC+AGG - chr7.4:4145731-4145750 MS.gene054429:CDS 50.0%
TCAGAGCTTGCATGGTCGTT+AGG - chr7.4:4144351-4144370 MS.gene054429:CDS 50.0%
TCAGCCTCTGTCATCCATTC+AGG + chr7.4:4144654-4144673 None:intergenic 50.0%
TCTCTGTGTCAGAGCTTGCA+TGG - chr7.4:4144343-4144362 MS.gene054429:CDS 50.0%
TGAATCGACACGCAATGCCA+CGG + chr7.4:4145189-4145208 None:intergenic 50.0%
TGCAGATGCAGTTGTTGCGT+TGG - chr7.4:4144710-4144729 MS.gene054429:intron 50.0%
TGGGAAGGGTTTCATTGCGA+GGG - chr7.4:4145121-4145140 MS.gene054429:CDS 50.0%
TTGCGAGGGACATGACATTC+CGG - chr7.4:4145135-4145154 MS.gene054429:CDS 50.0%
ACCGGTAAACCATCAAGCCG+TGG - chr7.4:4145169-4145188 MS.gene054429:CDS 55.0%
ATTCCGGAACACTGCTGGAC+CGG - chr7.4:4145151-4145170 MS.gene054429:CDS 55.0%
CATGGTCGTTAGGGGAGATG+AGG - chr7.4:4144361-4144380 MS.gene054429:CDS 55.0%
CCTCGAAGCATGGCTAAGTG+CGG - chr7.4:4144425-4144444 MS.gene054429:CDS 55.0%
CGCTCGCTGGTAGAGTCAAA+TGG - chr7.4:4145639-4145658 MS.gene054429:CDS 55.0%
CTGGGAAGGGTTTCATTGCG+AGG - chr7.4:4145120-4145139 MS.gene054429:CDS 55.0%
GACAGAACTGCGCAGTACGA+AGG - chr7.4:4144399-4144418 MS.gene054429:CDS 55.0%
GCAACCATGAACCGCCGTTA+AGG + chr7.4:4145724-4145743 None:intergenic 55.0%
GCTGGTAGAGTCAAATGGCC+TGG - chr7.4:4145644-4145663 MS.gene054429:CDS 55.0%
GGTTCCTTAACGGCGGTTCA+TGG - chr7.4:4145717-4145736 MS.gene054429:CDS 55.0%
GTATGGTTCAACCTCGAGGG+TGG - chr7.4:4145543-4145562 MS.gene054429:CDS 55.0%
GTTCAGCGGTTCCTTAACGG+CGG - chr7.4:4145710-4145729 MS.gene054429:CDS 55.0%
GTTGTTGCGTTGGATGGGAG+CGG - chr7.4:4144720-4144739 MS.gene054429:intron 55.0%
TCTACCAGCGAGCGATGAAC+CGG + chr7.4:4145633-4145652 None:intergenic 55.0%
TTACCGGTCCAGCAGTGTTC+CGG + chr7.4:4145157-4145176 None:intergenic 55.0%
! GCCACGGCTTGATGGTTTAC+CGG + chr7.4:4145173-4145192 None:intergenic 55.0%
AACCTCGAGGGTGGCTTGAG+TGG - chr7.4:4145552-4145571 MS.gene054429:CDS 60.0%
CACGCAATGCCACGGCTTGA+TGG + chr7.4:4145181-4145200 None:intergenic 60.0%
CCGCACTTAGCCATGCTTCG+AGG + chr7.4:4144428-4144447 None:intergenic 60.0%
CTACCAGCGAGCGATGAACC+GGG + chr7.4:4145632-4145651 None:intergenic 60.0%
GTACTGCGCAGTTCTGTCGC+CGG + chr7.4:4144397-4144416 None:intergenic 60.0%
TGGACTTGGAGGGCCTGTTC+TGG + chr7.4:4144184-4144203 None:intergenic 60.0%
AGCCACTCAAGCCACCCTCG+AGG + chr7.4:4145557-4145576 None:intergenic 65.0%
CGAGGGTGGCTTGAGTGGCT+CGG - chr7.4:4145557-4145576 MS.gene054429:CDS 65.0%
GGACCCGGTTCATCGCTCGC+TGG - chr7.4:4145626-4145645 MS.gene054429:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 4143997 4145784 4143997 ID=MS.gene054429
chr7.4 mRNA 4143997 4145784 4143997 ID=MS.gene054429.t1;Parent=MS.gene054429
chr7.4 exon 4144821 4145784 4144821 ID=MS.gene054429.t1.exon1;Parent=MS.gene054429.t1
chr7.4 CDS 4144821 4145784 4144821 ID=cds.MS.gene054429.t1;Parent=MS.gene054429.t1
chr7.4 exon 4143997 4144667 4143997 ID=MS.gene054429.t1.exon2;Parent=MS.gene054429.t1
chr7.4 CDS 4143997 4144667 4143997 ID=cds.MS.gene054429.t1;Parent=MS.gene054429.t1
Gene Sequence

>MS.gene054429

ATGGCCTTGCTTGTGAATTTCCTTTTCATTCTTATCCTTTTACCATCATTTGATCAAGTTCTCTCATTCCCAGATGAAATTCCCTCAGACATCCAAACTCAAGATATGCAAGCTTTGATTGCACAAGCTTGCATGGATATTGAAAACCAAAACTCCTGCCTCACAAACATTCATAACGAGCTAACCAGAACAGGCCCTCCAAGTCCAACTTCTGTCATTAATGCTGCACTTAGAACCACAATCAATGAAGCCATAGGTGCCATCAACAACATGACAAAGATCAGTACATTCTCTGTTAATAACCGCGAGCAACTTGCCATAGAAGATTGCAAGGAGCTCCTTGATTTCTCTGTGTCAGAGCTTGCATGGTCGTTAGGGGAGATGAGGAGAATTCGAGCCGGCGACAGAACTGCGCAGTACGAAGGAAACCTCGAAGCATGGCTAAGTGCGGCGCTTAGCAACCAAGACACATGTATTGAAGGGTTTGAAGGCACTGATAGACGTCTTGAGAGTTACATTAGTGGAAGCTTAACACAAGTAACCCAGCTTATCAGTAATGTTCTGTCTTTGTACACTCAGCTTAATCGCTTACCCTTTAGGTCTCCAAGGAACACTACCTTGCATGAAACTAGTACTGATGAAAGTTTGGAGTTTCCTGAATGGATGACAGAGGCTGATCAAGAGCTACTTAAATCTAAGCCGCACGGTAAGATTGCAGATGCAGTTGTTGCGTTGGATGGGAGCGGTCAGTACCGTACAATCAACGAAGCTGTTAATGCAGCTCCTAGTCATAGCAATAGGAGACATGTTATCTATGTGAAGAAGGGACTATACAAGGAGAATATTGATATGAAGAAGAAGATGACCAACATCATGATCGTTGGTGATGGTATTGGTCAAACCATTGTTACAAGCAATCGCAATTTCATGCAAGGATGGACCACTTTTCGAACTGCCACGTTTGGTAAGTAGAGTTCTTATTTAACAGTATATCTCTAGCGTAAGGAATTGAAATCTACGTGCTTTTTGTACTATCACCTACATATTGAAAAGAGTGTGTTAGAGAATGAATTGGTATATAGTGAATTGGTTGATAATTTGAAATGTTAATGTGTAGCTGTATCTGGGAAGGGTTTCATTGCGAGGGACATGACATTCCGGAACACTGCTGGACCGGTAAACCATCAAGCCGTGGCATTGCGTGTCGATTCAGACCAATCTGCATTCTTTAGGTGTAGCATAGAAGGAAACCAAGACACACTTTACGCACACTCACTGAGACAGTTCTACCGTGAATGCGAAATCTATGGAACCATAGACTTCATTTTTGGGAATGGAGCTGCTGTGCTACAAAACTGCAAGATATACACAAGAGTTCCACTTCCATTACAAAAGGTTACAATCACAGCTCAAGGTAGGAAAAGTCCACATCAAAGCACAGGTTTCACAATCCAAGATAGTTATGTTTTGGCCTCACAACCTACATATTTGGGGAGGCCTTGGAAGGAATATTCTAGAACAGTTTACATTAACACATACATGAGTAGTATGGTTCAACCTCGAGGGTGGCTTGAGTGGCTCGGTAACTTTGCATTAGACACTTTGTGGTACGGTGAATACAGGAATTATGGACCCGGTTCATCGCTCGCTGGTAGAGTCAAATGGCCTGGTTATCATGTCATTAAAGACGCTTCTGCCGCCGGTTATTTTACGGTTCAGCGGTTCCTTAACGGCGGTTCATGGTTGCCTAGAACAGGTGTTAAGTTCACAGCAGGGCTAAGCAATTGA
Protein sequence

>MS.gene054429.t1

MALLVNFLFILILLPSFDQVLSFPDEIPSDIQTQDMQALIAQACMDIENQNSCLTNIHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKISTFSVNNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCIEGFEGTDRRLESYISGSLTQVTQLISNVLSLYTQLNRLPFRSPRNTTLHETSTDESLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMIVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIARDMTFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLASQPTYLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGLSN