Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61742.t1 | RHN44133.1 | 95.6 | 90 | 4 | 0 | 1 | 90 | 98 | 187 | 2.20E-38 | 167.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61742.t1 | I3T6I6 | 95.6 | 90 | 4 | 0 | 1 | 90 | 77 | 166 | 1.6e-38 | 167.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene61742 | 0.834589 | 2.75E-56 | -1.69E-46 |
| MS.gene049531 | MS.gene61742 | 0.81679 | 4.61E-52 | -1.69E-46 |
| MS.gene049587 | MS.gene61742 | 0.81542 | 9.33E-52 | -1.69E-46 |
| MS.gene049701 | MS.gene61742 | 0.807776 | 4.28E-50 | -1.69E-46 |
| MS.gene049853 | MS.gene61742 | 0.828873 | 7.06E-55 | -1.69E-46 |
| MS.gene051710 | MS.gene61742 | 0.838041 | 3.64E-57 | -1.69E-46 |
| MS.gene053974 | MS.gene61742 | 0.818739 | 1.68E-52 | -1.69E-46 |
| MS.gene054801 | MS.gene61742 | 0.814454 | 1.53E-51 | -1.69E-46 |
| MS.gene055017 | MS.gene61742 | 0.850371 | 1.77E-60 | -1.69E-46 |
| MS.gene055056 | MS.gene61742 | 0.811938 | 5.45E-51 | -1.69E-46 |
| MS.gene055058 | MS.gene61742 | 0.830439 | 2.94E-55 | -1.69E-46 |
| MS.gene055091 | MS.gene61742 | 0.853897 | 1.76E-61 | -1.69E-46 |
| MS.gene058515 | MS.gene61742 | 0.878229 | 3.32E-69 | -1.69E-46 |
| MS.gene059276 | MS.gene61742 | 0.881164 | 3.01E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61742.t1 | MTR_2g026970 | 82.353 | 68 | 12 | 0 | 23 | 90 | 3 | 70 | 1.82e-35 | 118 |
| MS.gene61742.t1 | MTR_3g463980 | 83.871 | 62 | 10 | 0 | 29 | 90 | 73 | 134 | 2.95e-28 | 100 |
| MS.gene61742.t1 | MTR_3g005890 | 92.000 | 50 | 4 | 0 | 41 | 90 | 18 | 67 | 6.25e-23 | 85.5 |
| MS.gene61742.t1 | MTR_1g077860 | 65.517 | 58 | 19 | 1 | 20 | 77 | 56 | 112 | 5.76e-17 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGCATAACATTAAAAACTA+TGG | + | 23899:26939-26958 | None:intergenic | 20.0% |
| !!! | TTTTATGTTTATTTGACAGA+AGG | + | 23899:26700-26719 | None:intergenic | 20.0% |
| ! | AAAACAATTATAGCACATGA+TGG | + | 23899:26871-26890 | None:intergenic | 25.0% |
| ! | CTAGAATTAGTTATATCCTT+TGG | - | 23899:26738-26757 | MS.gene61742:CDS | 25.0% |
| !! | TCATGTGCTATAATTGTTTT+TGG | - | 23899:26871-26890 | MS.gene61742:CDS | 25.0% |
| AAAGGACACATATATGGTTT+GGG | - | 23899:27211-27230 | MS.gene61742:intron | 30.0% | |
| GAATTAGTTATATCCTTTGG+TGG | - | 23899:26741-26760 | MS.gene61742:CDS | 30.0% | |
| TAAAATGTCAGCTGAAAACA+TGG | - | 23899:27324-27343 | MS.gene61742:intron | 30.0% | |
| TGATCTTATGTTTCTTCACT+TGG | - | 23899:26998-27017 | MS.gene61742:intron | 30.0% | |
| TGTAGGATGAATATGAAAGA+GGG | - | 23899:27086-27105 | MS.gene61742:intron | 30.0% | |
| AAAAACTATGGCACAAAAGC+AGG | + | 23899:26927-26946 | None:intergenic | 35.0% | |
| AAGACGAACTCTAATTGTGT+AGG | - | 23899:27069-27088 | MS.gene61742:intron | 35.0% | |
| AAGGACACATATATGGTTTG+GGG | - | 23899:27212-27231 | MS.gene61742:intron | 35.0% | |
| ATGAAAGAGGGATCTTTGAA+AGG | - | 23899:27098-27117 | MS.gene61742:intron | 35.0% | |
| ATGTTTATTTGACAGAAGGC+TGG | + | 23899:26696-26715 | None:intergenic | 35.0% | |
| CATCTGAATATGAGCATATG+AGG | - | 23899:27290-27309 | MS.gene61742:intron | 35.0% | |
| CTCATATGCTCATATTCAGA+TGG | + | 23899:27292-27311 | None:intergenic | 35.0% | |
| GAAAGGACACATATATGGTT+TGG | - | 23899:27210-27229 | MS.gene61742:intron | 35.0% | |
| GACAAGAAAGGACACATATA+TGG | - | 23899:27205-27224 | MS.gene61742:intron | 35.0% | |
| GTGTAGGATGAATATGAAAG+AGG | - | 23899:27085-27104 | MS.gene61742:intron | 35.0% | |
| TCTTCATCCAGATCATTTGA+CGG | - | 23899:27259-27278 | MS.gene61742:intron | 35.0% | |
| AAGGAACTCACAACAAACAG+AGG | - | 23899:27117-27136 | MS.gene61742:intron | 40.0% | |
| AGATGAAGTTGTCTGACTGA+TGG | + | 23899:27162-27181 | None:intergenic | 40.0% | |
| AGCATGCATCAATACACATG+AGG | + | 23899:26831-26850 | None:intergenic | 40.0% | |
| AGGAACTCACAACAAACAGA+GGG | - | 23899:27118-27137 | MS.gene61742:intron | 40.0% | |
| CTGAAAACATGGAACTCTCA+AGG | - | 23899:27335-27354 | MS.gene61742:intron | 40.0% | |
| GTCAGACAACTTCATCTTCT+TGG | - | 23899:27165-27184 | MS.gene61742:intron | 40.0% | |
| TGAAATGCCGTCAAATGATC+TGG | + | 23899:27269-27288 | None:intergenic | 40.0% | |
| TGGTGGAAAGTCAAGGTAAT+TGG | - | 23899:26775-26794 | MS.gene61742:CDS | 40.0% | |
| TTCATCTTCTTGGACGTAGT+TGG | - | 23899:27175-27194 | MS.gene61742:intron | 40.0% | |
| !! | GTTATATCCTTTGGTGGACT+TGG | - | 23899:26747-26766 | MS.gene61742:CDS | 40.0% |
| AACATGGAACTCTCAAGGAG+CGG | - | 23899:27340-27359 | MS.gene61742:intron | 45.0% | |
| ATCTTCTTGGACGTAGTTGG+AGG | - | 23899:27178-27197 | MS.gene61742:intron | 45.0% | |
| GAACTCACAACAAACAGAGG+GGG | - | 23899:27120-27139 | MS.gene61742:intron | 45.0% | |
| GGAACTCACAACAAACAGAG+GGG | - | 23899:27119-27138 | MS.gene61742:intron | 45.0% | |
| ! | CATATATGGTTTGGGGACAG+AGG | - | 23899:27219-27238 | MS.gene61742:intron | 45.0% |
| CAGCATACCAAGTCCACCAA+AGG | + | 23899:26757-26776 | None:intergenic | 50.0% | |
| GGTATGCTGGTGGAAAGTCA+AGG | - | 23899:26768-26787 | MS.gene61742:CDS | 50.0% | |
| GTTGGAGGAGTCGACAAGAA+AGG | - | 23899:27193-27212 | MS.gene61742:intron | 50.0% | |
| !! | CTTTGGTGGACTTGGTATGC+TGG | - | 23899:26755-26774 | MS.gene61742:CDS | 50.0% |
| ! | TATGGTTTGGGGACAGAGGC+AGG | - | 23899:27223-27242 | MS.gene61742:intron | 55.0% |
| !! | TGGTGGACTTGGTATGCTGG+TGG | - | 23899:26758-26777 | MS.gene61742:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23899 | gene | 26654 | 27366 | 26654 | ID=MS.gene61742 |
| 23899 | mRNA | 26654 | 27366 | 26654 | ID=MS.gene61742.t1;Parent=MS.gene61742 |
| 23899 | exon | 27358 | 27366 | 27358 | ID=MS.gene61742.t1.exon1;Parent=MS.gene61742.t1 |
| 23899 | CDS | 27358 | 27366 | 27358 | ID=cds.MS.gene61742.t1;Parent=MS.gene61742.t1 |
| 23899 | exon | 26654 | 26929 | 26654 | ID=MS.gene61742.t1.exon2;Parent=MS.gene61742.t1 |
| 23899 | CDS | 26654 | 26929 | 26654 | ID=cds.MS.gene61742.t1;Parent=MS.gene61742.t1 |
| Gene Sequence |
| Protein sequence |
