Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78647.t1 | XP_004491126.1 | 68.2 | 88 | 22 | 3 | 16 | 99 | 102 | 187 | 4.50E-09 | 71.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78647.t1 | A0A1S2XNN8 | 68.2 | 88 | 22 | 3 | 16 | 99 | 102 | 187 | 3.3e-09 | 71.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene78647 | 0.839865 | 1.23E-57 | -1.69E-46 |
| MS.gene049587 | MS.gene78647 | 0.815138 | 1.08E-51 | -1.69E-46 |
| MS.gene049701 | MS.gene78647 | 0.814935 | 1.20E-51 | -1.69E-46 |
| MS.gene049853 | MS.gene78647 | 0.814912 | 1.21E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene78647 | 0.817227 | 3.68E-52 | -1.69E-46 |
| MS.gene050029 | MS.gene78647 | 0.805265 | 1.45E-49 | -1.69E-46 |
| MS.gene050266 | MS.gene78647 | 0.803362 | 3.61E-49 | -1.69E-46 |
| MS.gene050430 | MS.gene78647 | 0.812365 | 4.40E-51 | -1.69E-46 |
| MS.gene050611 | MS.gene78647 | 0.827002 | 1.99E-54 | -1.69E-46 |
| MS.gene051181 | MS.gene78647 | 0.804134 | 2.50E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene78647 | 0.823472 | 1.36E-53 | -1.69E-46 |
| MS.gene051932 | MS.gene78647 | 0.801587 | 8.39E-49 | -1.69E-46 |
| MS.gene052447 | MS.gene78647 | 0.825326 | 4.98E-54 | -1.69E-46 |
| MS.gene05329 | MS.gene78647 | 0.81256 | 3.99E-51 | -1.69E-46 |
| MS.gene053512 | MS.gene78647 | 0.830347 | 3.09E-55 | -1.69E-46 |
| MS.gene053646 | MS.gene78647 | 0.809307 | 2.02E-50 | -1.69E-46 |
| MS.gene053974 | MS.gene78647 | 0.840678 | 7.52E-58 | -1.69E-46 |
| MS.gene05414 | MS.gene78647 | 0.800066 | 1.72E-48 | -1.69E-46 |
| MS.gene05415 | MS.gene78647 | 0.814902 | 1.22E-51 | -1.69E-46 |
| MS.gene054174 | MS.gene78647 | 0.841897 | 3.60E-58 | -1.69E-46 |
| MS.gene054175 | MS.gene78647 | 0.846767 | 1.77E-59 | -1.69E-46 |
| MS.gene054291 | MS.gene78647 | -0.803828 | 2.89E-49 | -1.69E-46 |
| MS.gene05435 | MS.gene78647 | 0.849167 | 3.85E-60 | -1.69E-46 |
| MS.gene054484 | MS.gene78647 | 0.805804 | 1.12E-49 | -1.69E-46 |
| MS.gene055056 | MS.gene78647 | 0.85741 | 1.66E-62 | -1.69E-46 |
| MS.gene055058 | MS.gene78647 | 0.808952 | 2.41E-50 | -1.69E-46 |
| MS.gene055091 | MS.gene78647 | 0.806813 | 6.86E-50 | -1.69E-46 |
| MS.gene05762 | MS.gene78647 | 0.805047 | 1.61E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene78647 | 0.86479 | 9.44E-65 | -1.69E-46 |
| MS.gene05925 | MS.gene78647 | 0.81964 | 1.04E-52 | -1.69E-46 |
| MS.gene059276 | MS.gene78647 | 0.836927 | 7.02E-57 | -1.69E-46 |
| MS.gene059458 | MS.gene78647 | 0.814442 | 1.54E-51 | -1.69E-46 |
| MS.gene059459 | MS.gene78647 | 0.832003 | 1.21E-55 | -1.69E-46 |
| MS.gene059460 | MS.gene78647 | 0.806965 | 6.37E-50 | -1.69E-46 |
| MS.gene059584 | MS.gene78647 | 0.825925 | 3.59E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78647.t1 | MTR_5g085870 | 85.000 | 80 | 12 | 0 | 20 | 99 | 70 | 149 | 6.68e-29 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGAACTCGTAAGGGGTTT+AGG | 0.258839 | 1.4:-42418584 | None:intergenic |
| TCGAACTCGTAAGGGGTTTA+GGG | 0.305117 | 1.4:-42418583 | None:intergenic |
| TTTCTTACCAATTGACCTTC+GGG | 0.368480 | 1.4:-42418727 | None:intergenic |
| TGTGACAGCGATTGCGAAAG+AGG | 0.387116 | 1.4:-42418672 | None:intergenic |
| AGAGAATCGGAAGGTGAAAC+AGG | 0.391069 | 1.4:-42418621 | None:intergenic |
| CTTCGATCCCGAAGGTCAAT+TGG | 0.442039 | 1.4:+42418720 | MS.gene78647:CDS |
| AGTGTATGGTTGTAGGTTGC+TGG | 0.451119 | 1.4:+42419589 | MS.gene78647:CDS |
| TGTTAGAAGTGTATGGTTGT+AGG | 0.463926 | 1.4:+42419582 | MS.gene78647:CDS |
| AAATGTTAGCAAGGATGTGT+TGG | 0.480699 | 1.4:+42419768 | MS.gene78647:CDS |
| AATGTTAGCAAGGATGTGTT+GGG | 0.487842 | 1.4:+42419769 | MS.gene78647:CDS |
| ATTGGTAAGAAAAGCGGCTC+TGG | 0.492617 | 1.4:+42418738 | MS.gene78647:CDS |
| TGTGTTGGGTTCTACTATTG+AGG | 0.496016 | 1.4:+42419783 | MS.gene78647:CDS |
| CCCCTTAGAAAATGTTAGCA+AGG | 0.500090 | 1.4:+42419759 | MS.gene78647:CDS |
| ATATCAATGTTAGAAGTGTA+TGG | 0.508506 | 1.4:+42419575 | MS.gene78647:CDS |
| AAAATCACGTGAAAGAGAAT+CGG | 0.520635 | 1.4:-42418634 | None:intergenic |
| AGAGAGAAATCGAACTCGTA+AGG | 0.532543 | 1.4:-42418592 | None:intergenic |
| GAGAGAAATCGAACTCGTAA+GGG | 0.554082 | 1.4:-42418591 | None:intergenic |
| AGAAAAGCGGCTCTGGTAGT+CGG | 0.568712 | 1.4:+42418745 | MS.gene78647:CDS |
| GCGAAAGAGGAGAGTCATCA+TGG | 0.570240 | 1.4:-42418659 | None:intergenic |
| GCTCTGGTAGTCGGCAAAGT+GGG | 0.580582 | 1.4:+42418754 | MS.gene78647:CDS |
| GGCTCTGGTAGTCGGCAAAG+TGG | 0.629147 | 1.4:+42418753 | MS.gene78647:CDS |
| TGTATAAGCTTCGATCCCGA+AGG | 0.631456 | 1.4:+42418712 | MS.gene78647:CDS |
| AGGTCAATTGGTAAGAAAAG+CGG | 0.631571 | 1.4:+42418732 | MS.gene78647:CDS |
| ATTACGCTGGTGGTCAGAAG+AGG | 0.632700 | 1.4:+42418844 | MS.gene78647:CDS |
| AGAGAAATCGAACTCGTAAG+GGG | 0.643399 | 1.4:-42418590 | None:intergenic |
| TCGCAAGAGTATTACGCTGG+TGG | 0.647638 | 1.4:+42418834 | MS.gene78647:CDS |
| GAATCGCAAGAGTATTACGC+TGG | 0.665497 | 1.4:+42418831 | MS.gene78647:CDS |
| TCACGTGAAAGAGAATCGGA+AGG | 0.687567 | 1.4:-42418630 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCATTGTTAAATAAAAATT+AGG | - | chr1.4:42419144-42419163 | None:intergenic | 10.0% |
| !! | TAATGATTAATTGTAATATT+AGG | + | chr1.4:42418955-42418974 | MS.gene78647:intron | 10.0% |
| !! | TCATTGTTAAATAAAAATTA+GGG | - | chr1.4:42419143-42419162 | None:intergenic | 10.0% |
| !!! | ATTCATTGATTTAAATAATT+TGG | + | chr1.4:42419162-42419181 | MS.gene78647:intron | 10.0% |
| !! | GTAAAAATTAAAGAAGAAAT+GGG | + | chr1.4:42419733-42419752 | MS.gene78647:CDS | 15.0% |
| !! | TGTAAAAATTAAAGAAGAAA+TGG | + | chr1.4:42419732-42419751 | MS.gene78647:CDS | 15.0% |
| !! | TTAATTGTAATATTAGGTTT+AGG | + | chr1.4:42418961-42418980 | MS.gene78647:intron | 15.0% |
| !! | TTAGATATAGATTAACTAAA+AGG | - | chr1.4:42419917-42419936 | None:intergenic | 15.0% |
| !! | TTTAGTTAATCTATATCTAA+TGG | + | chr1.4:42419917-42419936 | MS.gene78647:intron | 15.0% |
| !!! | TTTTCATTGATTTTGATTAT+GGG | + | chr1.4:42418888-42418907 | MS.gene78647:intron | 15.0% |
| !! | ATGAGAACAATTATACAATA+AGG | - | chr1.4:42420043-42420062 | None:intergenic | 20.0% |
| !! | GAAACTTTAACTAATAGATT+TGG | + | chr1.4:42419504-42419523 | MS.gene78647:intron | 20.0% |
| !!! | AAGTTACATATTTTTGTTGT+TGG | + | chr1.4:42419956-42419975 | MS.gene78647:intron | 20.0% |
| !!! | AGTTACATATTTTTGTTGTT+GGG | + | chr1.4:42419957-42419976 | MS.gene78647:intron | 20.0% |
| !!! | CTTTTCATTGATTTTGATTA+TGG | + | chr1.4:42418887-42418906 | MS.gene78647:intron | 20.0% |
| !!! | TTGTCTGTTATTTGTTATTT+GGG | + | chr1.4:42419243-42419262 | MS.gene78647:intron | 20.0% |
| !!! | TTTGTCTGTTATTTGTTATT+TGG | + | chr1.4:42419242-42419261 | MS.gene78647:intron | 20.0% |
| ! | AAATCAATCAACTAACACTA+AGG | - | chr1.4:42419457-42419476 | None:intergenic | 25.0% |
| !! | ATATCAATGTTAGAAGTGTA+TGG | + | chr1.4:42419575-42419594 | MS.gene78647:CDS | 25.0% |
| !!! | ACTTAGGGTTTTAAGTTTAT+AGG | + | chr1.4:42418924-42418943 | MS.gene78647:intron | 25.0% |
| !!! | CTTAGGGTTTTAAGTTTATA+GGG | + | chr1.4:42418925-42418944 | MS.gene78647:intron | 25.0% |
| AAAATCACGTGAAAGAGAAT+CGG | - | chr1.4:42418637-42418656 | None:intergenic | 30.0% | |
| AATTGTTCTCATTGTTGTGT+AGG | + | chr1.4:42420051-42420070 | MS.gene78647:intron | 30.0% | |
| AGCAATATACTCCTAACAAT+AGG | - | chr1.4:42419639-42419658 | None:intergenic | 30.0% | |
| AGTTCCTTCATATTTCAGTT+AGG | + | chr1.4:42419106-42419125 | MS.gene78647:intron | 30.0% | |
| GAAACCTAACTGAAATATGA+AGG | - | chr1.4:42419113-42419132 | None:intergenic | 30.0% | |
| GGATATCAATTAGTAAGAGA+TGG | + | chr1.4:42419294-42419313 | MS.gene78647:intron | 30.0% | |
| TCAATTAGTAAGAGATGGTA+CGG | + | chr1.4:42419299-42419318 | MS.gene78647:intron | 30.0% | |
| TTGCATTCATTGAATTTGTC+AGG | + | chr1.4:42419705-42419724 | MS.gene78647:intron | 30.0% | |
| ! | ATCCTTGCTAACATTTTCTA+AGG | - | chr1.4:42419764-42419783 | None:intergenic | 30.0% |
| ! | CTTCATTTTGTTCTTGCATT+TGG | + | chr1.4:42419870-42419889 | MS.gene78647:intron | 30.0% |
| ! | TCCTTGCTAACATTTTCTAA+GGG | - | chr1.4:42419763-42419782 | None:intergenic | 30.0% |
| ! | TTTTCTTACCAATTGACCTT+CGG | - | chr1.4:42418731-42418750 | None:intergenic | 30.0% |
| !! | CTACAAAAGTACCTATTGTT+AGG | + | chr1.4:42419625-42419644 | MS.gene78647:intron | 30.0% |
| !!! | TGTTATTTGGGTTTTTGAGT+TGG | + | chr1.4:42419255-42419274 | MS.gene78647:intron | 30.0% |
| AAAGCTACATGTCATTGAAG+TGG | - | chr1.4:42419679-42419698 | None:intergenic | 35.0% | |
| AAATGTTAGCAAGGATGTGT+TGG | + | chr1.4:42419768-42419787 | MS.gene78647:CDS | 35.0% | |
| AATGTTAGCAAGGATGTGTT+GGG | + | chr1.4:42419769-42419788 | MS.gene78647:CDS | 35.0% | |
| AGATGGTACGGAATCAATTA+CGG | + | chr1.4:42419311-42419330 | MS.gene78647:intron | 35.0% | |
| AGGTCAATTGGTAAGAAAAG+CGG | + | chr1.4:42418732-42418751 | MS.gene78647:CDS | 35.0% | |
| TTTCTTACCAATTGACCTTC+GGG | - | chr1.4:42418730-42418749 | None:intergenic | 35.0% | |
| ! | CCTTGCTAACATTTTCTAAG+GGG | - | chr1.4:42419762-42419781 | None:intergenic | 35.0% |
| ! | TGTTAGAAGTGTATGGTTGT+AGG | + | chr1.4:42419582-42419601 | MS.gene78647:CDS | 35.0% |
| ! | TGTTAGGAGTATATTGCTGT+AGG | + | chr1.4:42419641-42419660 | MS.gene78647:intron | 35.0% |
| !!! | ATTTGGGTTTTTGAGTTGGT+TGG | + | chr1.4:42419259-42419278 | MS.gene78647:intron | 35.0% |
| AGAGAAATCGAACTCGTAAG+GGG | - | chr1.4:42418593-42418612 | None:intergenic | 40.0% | |
| AGAGAGAAATCGAACTCGTA+AGG | - | chr1.4:42418595-42418614 | None:intergenic | 40.0% | |
| CCCCTTAGAAAATGTTAGCA+AGG | + | chr1.4:42419759-42419778 | MS.gene78647:CDS | 40.0% | |
| GAGAGAAATCGAACTCGTAA+GGG | - | chr1.4:42418594-42418613 | None:intergenic | 40.0% | |
| GGAATGTGAGCGAATACTTA+GGG | + | chr1.4:42418909-42418928 | MS.gene78647:intron | 40.0% | |
| GGAGTATATTGCTGTAGGTT+CGG | + | chr1.4:42419646-42419665 | MS.gene78647:intron | 40.0% | |
| TGTGTTGGGTTCTACTATTG+AGG | + | chr1.4:42419783-42419802 | MS.gene78647:CDS | 40.0% | |
| TGTTGGGTTCACTTATGTGT+TGG | + | chr1.4:42419973-42419992 | MS.gene78647:intron | 40.0% | |
| AGAGAATCGGAAGGTGAAAC+AGG | - | chr1.4:42418624-42418643 | None:intergenic | 45.0% | |
| AGGCCTTCAATTGGATACGA+TGG | + | chr1.4:42419404-42419423 | MS.gene78647:intron | 45.0% | |
| AGTGTATGGTTGTAGGTTGC+TGG | + | chr1.4:42419589-42419608 | MS.gene78647:CDS | 45.0% | |
| AGTTGGTTGGAAGTTCTGCA+AGG | + | chr1.4:42419272-42419291 | MS.gene78647:intron | 45.0% | |
| ATCGAACTCGTAAGGGGTTT+AGG | - | chr1.4:42418587-42418606 | None:intergenic | 45.0% | |
| ATTGGTAAGAAAAGCGGCTC+TGG | + | chr1.4:42418738-42418757 | MS.gene78647:CDS | 45.0% | |
| GGGAATGTGAGCGAATACTT+AGG | + | chr1.4:42418908-42418927 | MS.gene78647:intron | 45.0% | |
| GTGCCATCGTATCCAATTGA+AGG | - | chr1.4:42419410-42419429 | None:intergenic | 45.0% | |
| GTTGGTTGGAAGTTCTGCAA+GGG | + | chr1.4:42419273-42419292 | MS.gene78647:intron | 45.0% | |
| TCACGTGAAAGAGAATCGGA+AGG | - | chr1.4:42418633-42418652 | None:intergenic | 45.0% | |
| TCGAACTCGTAAGGGGTTTA+GGG | - | chr1.4:42418586-42418605 | None:intergenic | 45.0% | |
| TGTATAAGCTTCGATCCCGA+AGG | + | chr1.4:42418712-42418731 | MS.gene78647:CDS | 45.0% | |
| !! | GAATCGCAAGAGTATTACGC+TGG | + | chr1.4:42418831-42418850 | MS.gene78647:CDS | 45.0% |
| ATTACGCTGGTGGTCAGAAG+AGG | + | chr1.4:42418844-42418863 | MS.gene78647:CDS | 50.0% | |
| CATGAGTGCAGGCCTTCAAT+TGG | + | chr1.4:42419395-42419414 | MS.gene78647:intron | 50.0% | |
| CTTCGATCCCGAAGGTCAAT+TGG | + | chr1.4:42418720-42418739 | MS.gene78647:CDS | 50.0% | |
| GAGTATGGTGACATGAGTGC+AGG | + | chr1.4:42419384-42419403 | MS.gene78647:intron | 50.0% | |
| GCAACGCTGACACTAGAGTA+TGG | + | chr1.4:42419369-42419388 | MS.gene78647:intron | 50.0% | |
| GCGAAAGAGGAGAGTCATCA+TGG | - | chr1.4:42418662-42418681 | None:intergenic | 50.0% | |
| GGTACGGAATCAATTACGGC+CGG | + | chr1.4:42419315-42419334 | MS.gene78647:intron | 50.0% | |
| TGTGACAGCGATTGCGAAAG+AGG | - | chr1.4:42418675-42418694 | None:intergenic | 50.0% | |
| ! | AGAAAAGCGGCTCTGGTAGT+CGG | + | chr1.4:42418745-42418764 | MS.gene78647:CDS | 50.0% |
| !! | TCGCAAGAGTATTACGCTGG+TGG | + | chr1.4:42418834-42418853 | MS.gene78647:CDS | 50.0% |
| GCTCTGGTAGTCGGCAAAGT+GGG | + | chr1.4:42418754-42418773 | MS.gene78647:CDS | 55.0% | |
| GCACCTCTAAGCCTTGGGAG+CGG | + | chr1.4:42419337-42419356 | MS.gene78647:intron | 60.0% | |
| ! | CTCCCAAGGCTTAGAGGTGC+CGG | - | chr1.4:42419337-42419356 | None:intergenic | 60.0% |
| ! | GGCTCTGGTAGTCGGCAAAG+TGG | + | chr1.4:42418753-42418772 | MS.gene78647:CDS | 60.0% |
| GGCCGGCACCTCTAAGCCTT+GGG | + | chr1.4:42419332-42419351 | MS.gene78647:intron | 65.0% | |
| TCTAAGCCTTGGGAGCGGCG+CGG | + | chr1.4:42419342-42419361 | MS.gene78647:intron | 65.0% | |
| CGGCCGGCACCTCTAAGCCT+TGG | + | chr1.4:42419331-42419350 | MS.gene78647:intron | 70.0% | |
| GCGCCGCTCCCAAGGCTTAG+AGG | - | chr1.4:42419343-42419362 | None:intergenic | 70.0% | |
| GCTTTGCCGCGCCGCTCCCA+AGG | - | chr1.4:42419351-42419370 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 42418567 | 42420087 | 42418567 | ID=MS.gene78647 |
| chr1.4 | mRNA | 42418567 | 42420087 | 42418567 | ID=MS.gene78647.t1;Parent=MS.gene78647 |
| chr1.4 | exon | 42418567 | 42418865 | 42418567 | ID=MS.gene78647.t1.exon1;Parent=MS.gene78647.t1 |
| chr1.4 | CDS | 42418567 | 42418865 | 42418567 | ID=cds.MS.gene78647.t1;Parent=MS.gene78647.t1 |
| chr1.4 | exon | 42419544 | 42419610 | 42419544 | ID=MS.gene78647.t1.exon2;Parent=MS.gene78647.t1 |
| chr1.4 | CDS | 42419544 | 42419610 | 42419544 | ID=cds.MS.gene78647.t1;Parent=MS.gene78647.t1 |
| chr1.4 | exon | 42419727 | 42419804 | 42419727 | ID=MS.gene78647.t1.exon3;Parent=MS.gene78647.t1 |
| chr1.4 | CDS | 42419727 | 42419804 | 42419727 | ID=cds.MS.gene78647.t1;Parent=MS.gene78647.t1 |
| chr1.4 | exon | 42420073 | 42420087 | 42420073 | ID=MS.gene78647.t1.exon4;Parent=MS.gene78647.t1 |
| chr1.4 | CDS | 42420073 | 42420087 | 42420073 | ID=cds.MS.gene78647.t1;Parent=MS.gene78647.t1 |
| Gene Sequence |
| Protein sequence |
