Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38429.t1 | PNY06164.1 | 80 | 90 | 17 | 1 | 33 | 122 | 618 | 706 | 9.70E-34 | 152.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38429.t1 | Q6A332 | 71.8 | 78 | 21 | 1 | 33 | 110 | 684 | 760 | 1.3e-26 | 120.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38429.t1 | A0A2K3NSZ7 | 80.0 | 90 | 17 | 1 | 33 | 122 | 618 | 706 | 7.0e-34 | 152.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene38429 | 0.819738 | 9.92E-53 | -1.69E-46 |
| MS.gene049587 | MS.gene38429 | 0.808816 | 2.57E-50 | -1.69E-46 |
| MS.gene049701 | MS.gene38429 | 0.843083 | 1.74E-58 | -1.69E-46 |
| MS.gene049853 | MS.gene38429 | 0.820078 | 8.29E-53 | -1.69E-46 |
| MS.gene050611 | MS.gene38429 | 0.822549 | 2.23E-53 | -1.69E-46 |
| MS.gene051710 | MS.gene38429 | 0.827128 | 1.86E-54 | -1.69E-46 |
| MS.gene052447 | MS.gene38429 | 0.80871 | 2.71E-50 | -1.69E-46 |
| MS.gene053512 | MS.gene38429 | 0.80076 | 1.24E-48 | -1.69E-46 |
| MS.gene053974 | MS.gene38429 | 0.807805 | 4.22E-50 | -1.69E-46 |
| MS.gene05404 | MS.gene38429 | 0.817702 | 2.88E-52 | -1.69E-46 |
| MS.gene054174 | MS.gene38429 | 0.83318 | 6.18E-56 | -1.69E-46 |
| MS.gene054175 | MS.gene38429 | 0.836799 | 7.57E-57 | -1.69E-46 |
| MS.gene05435 | MS.gene38429 | 0.805663 | 1.20E-49 | -1.69E-46 |
| MS.gene054484 | MS.gene38429 | 0.818845 | 1.58E-52 | -1.69E-46 |
| MS.gene054801 | MS.gene38429 | 0.815881 | 7.37E-52 | -1.69E-46 |
| MS.gene055056 | MS.gene38429 | 0.860097 | 2.62E-63 | -1.69E-46 |
| MS.gene055058 | MS.gene38429 | 0.812855 | 3.44E-51 | -1.69E-46 |
| MS.gene055091 | MS.gene38429 | 0.817927 | 2.56E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene38429 | 0.889807 | 1.74E-73 | -1.69E-46 |
| MS.gene059276 | MS.gene38429 | 0.850456 | 1.68E-60 | -1.69E-46 |
| MS.gene059584 | MS.gene38429 | 0.80937 | 1.96E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38429.t1 | MTR_4g119390 | 88.462 | 78 | 8 | 1 | 33 | 110 | 707 | 783 | 1.20e-45 | 157 |
| MS.gene38429.t1 | MTR_4g119390 | 88.462 | 78 | 8 | 1 | 33 | 110 | 742 | 818 | 1.20e-45 | 157 |
| MS.gene38429.t1 | MTR_1g083040 | 73.973 | 73 | 18 | 1 | 33 | 105 | 694 | 765 | 1.87e-32 | 120 |
| MS.gene38429.t1 | MTR_1g083040 | 73.973 | 73 | 18 | 1 | 33 | 105 | 694 | 765 | 1.91e-32 | 120 |
| MS.gene38429.t1 | MTR_1g083040 | 73.973 | 73 | 18 | 1 | 33 | 105 | 694 | 765 | 1.91e-32 | 120 |
| MS.gene38429.t1 | MTR_1g083040 | 73.973 | 73 | 18 | 1 | 33 | 105 | 694 | 765 | 1.91e-32 | 120 |
| MS.gene38429.t1 | MTR_1g083040 | 73.973 | 73 | 18 | 1 | 33 | 105 | 694 | 765 | 1.91e-32 | 120 |
| MS.gene38429.t1 | MTR_6g086600 | 57.377 | 61 | 20 | 3 | 2 | 62 | 187 | 241 | 1.05e-12 | 63.5 |
| MS.gene38429.t1 | MTR_6g086600 | 57.377 | 61 | 20 | 3 | 2 | 62 | 178 | 232 | 1.22e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38429.t1 | AT3G21430 | 71.795 | 78 | 21 | 1 | 33 | 110 | 680 | 756 | 1.54e-34 | 126 |
| MS.gene38429.t1 | AT3G21430 | 71.795 | 78 | 21 | 1 | 33 | 110 | 680 | 756 | 1.54e-34 | 126 |
| MS.gene38429.t1 | AT3G21430 | 71.795 | 78 | 21 | 1 | 33 | 110 | 684 | 760 | 1.54e-34 | 126 |
| MS.gene38429.t1 | AT3G05380 | 71.233 | 73 | 18 | 2 | 33 | 105 | 634 | 703 | 6.03e-28 | 107 |
| MS.gene38429.t1 | AT3G05380 | 71.233 | 73 | 18 | 2 | 33 | 105 | 711 | 780 | 6.06e-28 | 107 |
| MS.gene38429.t1 | AT3G05380 | 71.233 | 73 | 18 | 2 | 33 | 105 | 712 | 781 | 6.06e-28 | 107 |
| MS.gene38429.t1 | AT3G05380 | 71.233 | 73 | 18 | 2 | 33 | 105 | 712 | 781 | 6.06e-28 | 107 |
| MS.gene38429.t1 | AT3G05380 | 71.233 | 73 | 18 | 2 | 33 | 105 | 712 | 781 | 6.06e-28 | 107 |
| MS.gene38429.t1 | AT5G27610 | 70.588 | 68 | 17 | 2 | 38 | 105 | 640 | 704 | 4.31e-23 | 93.6 |
| MS.gene38429.t1 | AT3G24430 | 54.098 | 61 | 22 | 3 | 2 | 62 | 186 | 240 | 5.11e-11 | 58.9 |
Find 38 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTATACTTTGGCTGATA+TGG | 0.137501 | 3.4:+63476849 | MS.gene38429:CDS |
| ATATTTGATGCTGATATTTA+TGG | 0.224569 | 3.4:+63476886 | MS.gene38429:CDS |
| ACACGCTGCCCAACAATTAA+TGG | 0.282373 | 3.4:-63476943 | None:intergenic |
| TCATGAATCTCCCTTGCCTT+TGG | 0.311415 | 3.4:-63476979 | None:intergenic |
| CTCCCTTGCCTTTGGATGAA+TGG | 0.327447 | 3.4:-63476971 | None:intergenic |
| CAGTTTGACCATCATGAACT+TGG | 0.327927 | 3.4:+63477045 | MS.gene38429:CDS |
| GCTTATACTTTGGCTGATAT+GGG | 0.360827 | 3.4:+63476850 | MS.gene38429:CDS |
| GGAAGGCCTACCTGCTGATT+TGG | 0.362133 | 3.4:+63476915 | MS.gene38429:CDS |
| ACTTTCAAAATCCAAGTTGC+TGG | 0.376954 | 3.4:-63477321 | None:intergenic |
| TGTAAACCTTGCTTATACTT+TGG | 0.395479 | 3.4:+63476840 | MS.gene38429:CDS |
| ATGGTGTCTTACCACAGATA+TGG | 0.421368 | 3.4:-63477124 | None:intergenic |
| TGGCTCGACCATTAATTGTT+GGG | 0.454205 | 3.4:+63476935 | MS.gene38429:CDS |
| TTGGCTCGACCATTAATTGT+TGG | 0.458768 | 3.4:+63476934 | MS.gene38429:CDS |
| CTGTTGACCATTGTAGATAC+CGG | 0.470249 | 3.4:+63477019 | MS.gene38429:CDS |
| AGTTTGACCATCATGAACTT+GGG | 0.485776 | 3.4:+63477046 | MS.gene38429:CDS |
| ATCAGCAGGTAGGCCTTCCT+TGG | 0.487896 | 3.4:-63476911 | None:intergenic |
| TGGTGTCTTACCACAGATAT+GGG | 0.500136 | 3.4:-63477123 | None:intergenic |
| GATATTTATGGTACCAAGGA+AGG | 0.500761 | 3.4:+63476898 | MS.gene38429:CDS |
| GGTGAAGGTACGATTAAAGA+TGG | 0.504065 | 3.4:+63477288 | MS.gene38429:intron |
| GAGAATATAATCCAGCAACT+TGG | 0.544718 | 3.4:+63477310 | MS.gene38429:CDS |
| GCTGATATGGGTGCTAGAGT+TGG | 0.558279 | 3.4:+63476862 | MS.gene38429:CDS |
| TGCCATTCATCCAAAGGCAA+GGG | 0.561305 | 3.4:+63476969 | MS.gene38429:CDS |
| TTGCCATTCATCCAAAGGCA+AGG | 0.563321 | 3.4:+63476968 | MS.gene38429:CDS |
| AATGGTCGAGCCAAATCAGC+AGG | 0.563507 | 3.4:-63476925 | None:intergenic |
| GTTTGACCATCATGAACTTG+GGG | 0.569708 | 3.4:+63477047 | MS.gene38429:CDS |
| TCCAGTTGCATGGGTGGTGA+AGG | 0.574912 | 3.4:+63476802 | None:intergenic |
| CATGATGGTCAAACTGAACC+CGG | 0.578855 | 3.4:-63477038 | None:intergenic |
| ACTTGGGGTTGAATTTGTCA+TGG | 0.578918 | 3.4:+63477062 | MS.gene38429:CDS |
| GCTGTATCCAGTTGCATGGG+TGG | 0.587759 | 3.4:+63476796 | None:intergenic |
| TGTTATTGCCATTCATCCAA+AGG | 0.591987 | 3.4:+63476963 | MS.gene38429:CDS |
| TGAAAATATGCCCATATCTG+TGG | 0.598281 | 3.4:+63477113 | MS.gene38429:CDS |
| TGTTGACCATTGTAGATACC+GGG | 0.601453 | 3.4:+63477020 | MS.gene38429:CDS |
| AAGGCAAGGGAGATTCATGA+TGG | 0.610859 | 3.4:+63476982 | MS.gene38429:CDS |
| TGCTGATATTTATGGTACCA+AGG | 0.612578 | 3.4:+63476894 | MS.gene38429:CDS |
| ATTCAACCCCAAGTTCATGA+TGG | 0.625478 | 3.4:-63477053 | None:intergenic |
| TATCAGCCAAAGTATAAGCA+AGG | 0.626958 | 3.4:-63476846 | None:intergenic |
| ACTGAACCCGGTATCTACAA+TGG | 0.651577 | 3.4:-63477026 | None:intergenic |
| GTCGAGCCAAATCAGCAGGT+AGG | 0.699895 | 3.4:-63476921 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCTAATTAAATTAGCATAA+TGG | - | chr3.4:63477251-63477270 | None:intergenic | 15.0% |
| !! | ATATTTGATGCTGATATTTA+TGG | + | chr3.4:63476886-63476905 | MS.gene38429:CDS | 20.0% |
| !! | ATTTAATTAGATACTTTGAG+AGG | + | chr3.4:63477259-63477278 | MS.gene38429:intron | 20.0% |
| !! | TCAATTAATATATGAACGTT+GGG | - | chr3.4:63477162-63477181 | None:intergenic | 20.0% |
| !! | TTCAATTAATATATGAACGT+TGG | - | chr3.4:63477163-63477182 | None:intergenic | 20.0% |
| !!! | TTTAATTAGATACTTTGAGA+GGG | + | chr3.4:63477260-63477279 | MS.gene38429:intron | 20.0% |
| !! | ATATTTTCAGATGGATACAA+AGG | - | chr3.4:63477102-63477121 | None:intergenic | 25.0% |
| TGTAAACCTTGCTTATACTT+TGG | + | chr3.4:63476840-63476859 | MS.gene38429:CDS | 30.0% | |
| AGTTTGACCATCATGAACTT+GGG | + | chr3.4:63477046-63477065 | MS.gene38429:CDS | 35.0% | |
| CGTTGGGATGATAAATGATA+TGG | - | chr3.4:63477146-63477165 | None:intergenic | 35.0% | |
| GAGAATATAATCCAGCAACT+TGG | + | chr3.4:63477310-63477329 | MS.gene38429:CDS | 35.0% | |
| GATATTTATGGTACCAAGGA+AGG | + | chr3.4:63476898-63476917 | MS.gene38429:CDS | 35.0% | |
| GCTTATACTTTGGCTGATAT+GGG | + | chr3.4:63476850-63476869 | MS.gene38429:CDS | 35.0% | |
| TATCAGCCAAAGTATAAGCA+AGG | - | chr3.4:63476849-63476868 | None:intergenic | 35.0% | |
| TGAAAATATGCCCATATCTG+TGG | + | chr3.4:63477113-63477132 | MS.gene38429:CDS | 35.0% | |
| TGCTGATATTTATGGTACCA+AGG | + | chr3.4:63476894-63476913 | MS.gene38429:CDS | 35.0% | |
| TGCTTATACTTTGGCTGATA+TGG | + | chr3.4:63476849-63476868 | MS.gene38429:CDS | 35.0% | |
| TGTTATTGCCATTCATCCAA+AGG | + | chr3.4:63476963-63476982 | MS.gene38429:CDS | 35.0% | |
| ! | AAGCTTTTCCTGTTGAAGAA+AGG | + | chr3.4:63477192-63477211 | MS.gene38429:intron | 35.0% |
| ! | AGATACTTTGAGAGGGTTAA+AGG | + | chr3.4:63477267-63477286 | MS.gene38429:intron | 35.0% |
| ! | GATATGGGCATATTTTCAGA+TGG | - | chr3.4:63477111-63477130 | None:intergenic | 35.0% |
| ACTTGGGGTTGAATTTGTCA+TGG | + | chr3.4:63477062-63477081 | MS.gene38429:CDS | 40.0% | |
| ATGGTGTCTTACCACAGATA+TGG | - | chr3.4:63477127-63477146 | None:intergenic | 40.0% | |
| ATTCAACCCCAAGTTCATGA+TGG | - | chr3.4:63477056-63477075 | None:intergenic | 40.0% | |
| CAGTTTGACCATCATGAACT+TGG | + | chr3.4:63477045-63477064 | MS.gene38429:CDS | 40.0% | |
| CTGTTGACCATTGTAGATAC+CGG | + | chr3.4:63477019-63477038 | MS.gene38429:CDS | 40.0% | |
| GAGGACAAGAGAAACATGTT+GGG | + | chr3.4:63477214-63477233 | MS.gene38429:intron | 40.0% | |
| GTTTGACCATCATGAACTTG+GGG | + | chr3.4:63477047-63477066 | MS.gene38429:CDS | 40.0% | |
| TGGCTCGACCATTAATTGTT+GGG | + | chr3.4:63476935-63476954 | MS.gene38429:CDS | 40.0% | |
| TGGTGTCTTACCACAGATAT+GGG | - | chr3.4:63477126-63477145 | None:intergenic | 40.0% | |
| TGTTGACCATTGTAGATACC+GGG | + | chr3.4:63477020-63477039 | MS.gene38429:CDS | 40.0% | |
| TTAGCATAATGGTGTCCAAG+TGG | - | chr3.4:63477240-63477259 | None:intergenic | 40.0% | |
| TTTGAGAGGGTTAAAGGTGA+AGG | + | chr3.4:63477273-63477292 | MS.gene38429:intron | 40.0% | |
| ! | CTTTTCCTGTTGAAGAAAGG+AGG | + | chr3.4:63477195-63477214 | MS.gene38429:intron | 40.0% |
| ! | GGTGAAGGTACGATTAAAGA+TGG | + | chr3.4:63477288-63477307 | MS.gene38429:intron | 40.0% |
| ! | TTGGCTCGACCATTAATTGT+TGG | + | chr3.4:63476934-63476953 | MS.gene38429:CDS | 40.0% |
| AAGGCAAGGGAGATTCATGA+TGG | + | chr3.4:63476982-63477001 | MS.gene38429:CDS | 45.0% | |
| ACACGCTGCCCAACAATTAA+TGG | - | chr3.4:63476946-63476965 | None:intergenic | 45.0% | |
| ACTGAACCCGGTATCTACAA+TGG | - | chr3.4:63477029-63477048 | None:intergenic | 45.0% | |
| CATGATGGTCAAACTGAACC+CGG | - | chr3.4:63477041-63477060 | None:intergenic | 45.0% | |
| CTTGTCCTCCTTTCTTCAAC+AGG | - | chr3.4:63477203-63477222 | None:intergenic | 45.0% | |
| GGAGGACAAGAGAAACATGT+TGG | + | chr3.4:63477213-63477232 | MS.gene38429:intron | 45.0% | |
| TCATGAATCTCCCTTGCCTT+TGG | - | chr3.4:63476982-63477001 | None:intergenic | 45.0% | |
| TGCCATTCATCCAAAGGCAA+GGG | + | chr3.4:63476969-63476988 | MS.gene38429:CDS | 45.0% | |
| TTGCCATTCATCCAAAGGCA+AGG | + | chr3.4:63476968-63476987 | MS.gene38429:CDS | 45.0% | |
| AATGGTCGAGCCAAATCAGC+AGG | - | chr3.4:63476928-63476947 | None:intergenic | 50.0% | |
| CTCCCTTGCCTTTGGATGAA+TGG | - | chr3.4:63476974-63476993 | None:intergenic | 50.0% | |
| GAGAAACATGTTGGGCCACT+TGG | + | chr3.4:63477222-63477241 | MS.gene38429:intron | 50.0% | |
| GCTGATATGGGTGCTAGAGT+TGG | + | chr3.4:63476862-63476881 | MS.gene38429:CDS | 50.0% | |
| ATCAGCAGGTAGGCCTTCCT+TGG | - | chr3.4:63476914-63476933 | None:intergenic | 55.0% | |
| GTCGAGCCAAATCAGCAGGT+AGG | - | chr3.4:63476924-63476943 | None:intergenic | 55.0% | |
| ! | GGAAGGCCTACCTGCTGATT+TGG | + | chr3.4:63476915-63476934 | MS.gene38429:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 63476811 | 63477339 | 63476811 | ID=MS.gene38429 |
| chr3.4 | mRNA | 63476811 | 63477339 | 63476811 | ID=MS.gene38429.t1;Parent=MS.gene38429 |
| chr3.4 | exon | 63476811 | 63477134 | 63476811 | ID=MS.gene38429.t1.exon1;Parent=MS.gene38429.t1 |
| chr3.4 | CDS | 63476811 | 63477134 | 63476811 | ID=cds.MS.gene38429.t1;Parent=MS.gene38429.t1 |
| chr3.4 | exon | 63477289 | 63477339 | 63477289 | ID=MS.gene38429.t1.exon2;Parent=MS.gene38429.t1 |
| chr3.4 | CDS | 63477289 | 63477339 | 63477289 | ID=cds.MS.gene38429.t1;Parent=MS.gene38429.t1 |
| Gene Sequence |
| Protein sequence |
