AlfalfaGEDB | MS.gene85151

M. sativa cultivar XinJiangDaYe / MS.gene85151

KEGG: -
GO: -
PFAM: PF07911.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85151.t1	XP_003597686.1	98.5	196	3	0	1	196	1	196	5.70E-105	390.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85151.t1	G7II08	98.5	196	3	0	1	196	1	196	4.1e-105	390.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene85151	0.858813	6.37E-63	-1.69E-46
MS.gene049369	MS.gene85151	0.833645	4.74E-56	-1.69E-46
MS.gene049396	MS.gene85151	0.807943	3.95E-50	-1.69E-46
MS.gene050122	MS.gene85151	0.805854	1.09E-49	-1.69E-46
MS.gene050128	MS.gene85151	0.808227	3.44E-50	-1.69E-46
MS.gene050670	MS.gene85151	0.844077	9.45E-59	-1.69E-46
MS.gene050971	MS.gene85151	0.819094	1.39E-52	-1.69E-46
MS.gene05105	MS.gene85151	0.834602	2.73E-56	-1.69E-46
MS.gene052188	MS.gene85151	0.859552	3.82E-63	-1.69E-46
MS.gene052253	MS.gene85151	0.833921	4.04E-56	-1.69E-46
MS.gene052340	MS.gene85151	0.829215	5.83E-55	-1.69E-46
MS.gene052414	MS.gene85151	0.81822	2.20E-52	-1.69E-46
MS.gene052485	MS.gene85151	0.829355	5.39E-55	-1.69E-46
MS.gene053169	MS.gene85151	0.81143	7.03E-51	-1.69E-46
MS.gene05316	MS.gene85151	0.8392	1.83E-57	-1.69E-46
MS.gene053449	MS.gene85151	0.833082	6.54E-56	-1.69E-46
MS.gene053758	MS.gene85151	0.824476	7.91E-54	-1.69E-46
MS.gene053813	MS.gene85151	0.816651	4.96E-52	-1.69E-46
MS.gene054349	MS.gene85151	0.831868	1.31E-55	-1.69E-46
MS.gene054556	MS.gene85151	0.819612	1.06E-52	-1.69E-46
MS.gene055116	MS.gene85151	0.857724	1.34E-62	-1.69E-46
MS.gene055744	MS.gene85151	0.801413	9.11E-49	-1.69E-46
MS.gene055909	MS.gene85151	0.855576	5.75E-62	-1.69E-46
MS.gene055911	MS.gene85151	0.859945	2.91E-63	-1.69E-46
MS.gene056422	MS.gene85151	0.825274	5.13E-54	-1.69E-46
MS.gene056511	MS.gene85151	0.847634	1.02E-59	-1.69E-46
MS.gene057396	MS.gene85151	0.832242	1.06E-55	-1.69E-46
MS.gene057448	MS.gene85151	0.845105	5.00E-59	-1.69E-46
MS.gene057449	MS.gene85151	0.840094	1.07E-57	-1.69E-46
MS.gene057450	MS.gene85151	0.845632	3.60E-59	-1.69E-46
MS.gene057537	MS.gene85151	0.804412	2.19E-49	-1.69E-46
MS.gene057610	MS.gene85151	0.80503	1.63E-49	-1.69E-46
MS.gene057734	MS.gene85151	0.804132	2.50E-49	-1.69E-46
MS.gene057749	MS.gene85151	0.829107	6.19E-55	-1.69E-46
MS.gene057823	MS.gene85151	0.842785	2.09E-58	-1.69E-46
MS.gene058007	MS.gene85151	0.846532	2.05E-59	-1.69E-46
MS.gene058072	MS.gene85151	0.802938	4.42E-49	-1.69E-46
MS.gene058075	MS.gene85151	0.827497	1.51E-54	-1.69E-46
MS.gene058700	MS.gene85151	0.809763	1.61E-50	-1.69E-46
MS.gene058928	MS.gene85151	0.834762	2.49E-56	-1.69E-46
MS.gene058949	MS.gene85151	0.819522	1.11E-52	-1.69E-46
MS.gene05907	MS.gene85151	0.808217	3.45E-50	-1.69E-46
MS.gene059080	MS.gene85151	0.80584	1.10E-49	-1.69E-46
MS.gene059082	MS.gene85151	0.808829	2.56E-50	-1.69E-46
MS.gene059294	MS.gene85151	0.819816	9.52E-53	-1.69E-46
MS.gene059366	MS.gene85151	0.852366	4.84E-61	-1.69E-46
MS.gene059420	MS.gene85151	0.836585	8.59E-57	-1.69E-46
MS.gene059462	MS.gene85151	0.824723	6.91E-54	-1.69E-46
MS.gene059674	MS.gene85151	0.8603	2.27E-63	-1.69E-46
MS.gene059688	MS.gene85151	0.844253	8.48E-59	-1.69E-46
MS.gene059692	MS.gene85151	0.838359	3.01E-57	-1.69E-46
MS.gene059695	MS.gene85151	0.839762	1.31E-57	-1.69E-46
MS.gene059711	MS.gene85151	0.80196	7.03E-49	-1.69E-46
MS.gene059807	MS.gene85151	0.901	4.17E-78	-1.69E-46
MS.gene059876	MS.gene85151	0.826394	2.78E-54	-1.69E-46
MS.gene059933	MS.gene85151	0.815626	8.39E-52	-1.69E-46
MS.gene060372	MS.gene85151	0.812265	4.63E-51	-1.69E-46
MS.gene060403	MS.gene85151	0.807832	4.17E-50	-1.69E-46
MS.gene060495	MS.gene85151	0.816905	4.34E-52	-1.69E-46
MS.gene060837	MS.gene85151	0.835887	1.29E-56	-1.69E-46
MS.gene061154	MS.gene85151	0.829134	6.10E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85151.t1	MTR_2g101140	98.469	196	3	0	1	196	1	196	7.84e-145	400
MS.gene85151.t1	MTR_8g013990	67.839	199	54	5	1	196	3	194	6.62e-92	267
MS.gene85151.t1	MTR_8g021190	42.969	128	69	3	61	186	29	154	2.36e-29	106
MS.gene85151.t1	MTR_1g103550	47.191	89	41	2	62	148	2	86	2.69e-22	87.8
MS.gene85151.t1	MTR_5g006430	38.053	113	65	2	62	173	2	110	1.64e-20	82.8
MS.gene85151.t1	MTR_3g105340	52.778	72	33	1	52	122	1	72	2.76e-20	82.8
MS.gene85151.t1	MTR_3g109860	34.959	123	77	3	32	151	4	126	1.43e-17	75.9
MS.gene85151.t1	MTR_4g125010	50.725	69	33	1	61	128	23	91	6.59e-17	74.7
MS.gene85151.t1	MTR_4g125030	42.708	96	47	3	61	151	21	113	3.96e-16	71.6
MS.gene85151.t1	MTR_1g014930	41.111	90	39	3	53	135	4	86	2.51e-15	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85151.t1	AT5G25840	50.276	181	59	4	36	196	4	173	5.19e-53	167
MS.gene85151.t1	AT1G79770	53.205	156	59	3	35	190	26	167	1.39e-48	156
MS.gene85151.t1	AT1G72510	33.537	164	90	5	39	186	5	165	1.16e-22	89.7
MS.gene85151.t1	AT1G72510	33.537	164	90	5	39	186	5	165	1.16e-22	89.7
MS.gene85151.t1	AT4G14819	40.777	103	50	3	62	164	2	93	5.63e-22	86.3
MS.gene85151.t1	AT3G22540	40.449	89	50	1	62	150	2	87	2.20e-21	85.1
MS.gene85151.t1	AT2G25780	36.250	160	88	5	31	185	1	151	1.03e-20	84.3
MS.gene85151.t1	AT2G09970	31.707	164	92	5	40	186	6	166	1.44e-20	84.3
MS.gene85151.t1	AT1G54095	37.410	139	75	4	61	189	14	150	5.96e-20	82.4
MS.gene85151.t1	AT5G20670	35.878	131	76	5	63	186	23	152	4.80e-18	77.4

Find 34 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGTTAGATTTAATAAATT+TGG	0.209428	2.4:+3486096	MS.gene85151:CDS
AGCTTCTATATAAGATTAAT+TGG	0.236977	2.4:-3486307	None:intergenic
GCATCTTCAACTTCAGTTAT+CGG	0.334901	2.4:-3485898	None:intergenic
GTGATAAATACTTAGGGAAA+TGG	0.349825	2.4:+3485980	MS.gene85151:CDS
AGTTGAGATGAATTAGGTTT+TGG	0.358947	2.4:-3485793	None:intergenic
GCTTCAGCTTGAAACAAAAC+AGG	0.390939	2.4:-3486129	None:intergenic
CGTGTACGTGATAAATACTT+AGG	0.397786	2.4:+3485973	MS.gene85151:CDS
TTGTTGAGTTGAGATGAATT+AGG	0.428907	2.4:-3485799	None:intergenic
TTTCACCACCTTTGTCTCTA+GGG	0.429554	2.4:-3486209	None:intergenic
TGATAAATACTTAGGGAAAT+GGG	0.445526	2.4:+3485981	MS.gene85151:CDS
TGAGGCAGTGAAAGAAGAGT+TGG	0.465910	2.4:+3486020	MS.gene85151:CDS
TTTGGAGCATACAATGTTGT+TGG	0.466523	2.4:-3485767	None:intergenic
TACTTAGGGAAATGGGTTTG+TGG	0.466697	2.4:+3485988	MS.gene85151:CDS
GAGTAAAATGGAAGGGAGAA+GGG	0.485393	2.4:+3486173	MS.gene85151:CDS
TTGATATCTCTTGTAATAGC+AGG	0.500356	2.4:-3486270	None:intergenic
AGAGTAAAATGGAAGGGAGA+AGG	0.512738	2.4:+3486172	MS.gene85151:CDS
GTGTACGTGATAAATACTTA+GGG	0.514569	2.4:+3485974	MS.gene85151:CDS
GGTGGTGAAAAGAGAGGTGG+TGG	0.519798	2.4:+3486222	MS.gene85151:CDS
TCAACTATTATGGCACCTCA+TGG	0.537712	2.4:+3485715	None:intergenic
GTGGAAGCTGTGTTAAATCA+AGG	0.538899	2.4:-3485876	None:intergenic
TTAGATTTAATAAATTTGGA+AGG	0.546403	2.4:+3486100	MS.gene85151:CDS
CATTACCTAGCTCAATCTCT+AGG	0.548665	2.4:+3485743	MS.gene85151:CDS
TTGGTCCTAGAGATTGAGCT+AGG	0.570237	2.4:-3485748	None:intergenic
GGTTTGTGGTTTGTGTTCTG+AGG	0.597680	2.4:+3486002	MS.gene85151:CDS
GACAAAGGTGGTGAAAAGAG+AGG	0.597763	2.4:+3486216	MS.gene85151:CDS
TGGTGGGGAAAATATTGAAG+AGG	0.600750	2.4:+3486050	MS.gene85151:CDS
AAAGGTGGTGAAAAGAGAGG+TGG	0.623840	2.4:+3486219	MS.gene85151:CDS
TCTTCAACTTCAGTTATCGG+TGG	0.626778	2.4:-3485895	None:intergenic
TCATTCAACCCTAGAGACAA+AGG	0.638410	2.4:+3486201	MS.gene85151:CDS
CTTCAGCTTGAAACAAAACA+GGG	0.640341	2.4:-3486128	None:intergenic
GATGCAAAGTGTGAATGTTG+TGG	0.690241	2.4:+3485916	MS.gene85151:CDS
GTGGTGAAAAGAGAGGTGGT+GGG	0.706908	2.4:+3486223	MS.gene85151:CDS
TTCAACCCTAGAGACAAAGG+TGG	0.741656	2.4:+3486204	MS.gene85151:CDS
CTAGGTAATGAATCTCCATG+AGG	0.751297	2.4:-3485730	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTGTTAGATTTAATAAATT+TGG	+	chr2.4:3486096-3486115	MS.gene85151:CDS	15.0%
!!	TTAGATTTAATAAATTTGGA+AGG	+	chr2.4:3486100-3486119	MS.gene85151:CDS	15.0%
!	TGAAAAAGAGTAAAATGGAA+GGG	+	chr2.4:3486166-3486185	MS.gene85151:CDS	25.0%
!	TGATAAATACTTAGGGAAAT+GGG	+	chr2.4:3485981-3486000	MS.gene85151:CDS	25.0%
	AAAGAAGAGTTGGAGAAAAA+TGG	+	chr2.4:3486030-3486049	MS.gene85151:CDS	30.0%
	CTGAAAAAGAGTAAAATGGA+AGG	+	chr2.4:3486165-3486184	MS.gene85151:CDS	30.0%
	GATGCTGAAAAAGAGTAAAA+TGG	+	chr2.4:3486161-3486180	MS.gene85151:CDS	30.0%
	GTGATAAATACTTAGGGAAA+TGG	+	chr2.4:3485980-3485999	MS.gene85151:CDS	30.0%
	GTGTACGTGATAAATACTTA+GGG	+	chr2.4:3485974-3485993	MS.gene85151:CDS	30.0%
	TTGATATCTCTTGTAATAGC+AGG	-	chr2.4:3486273-3486292	None:intergenic	30.0%
!	AGTTGAGATGAATTAGGTTT+TGG	-	chr2.4:3485796-3485815	None:intergenic	30.0%
!!	TTGTTGAGTTGAGATGAATT+AGG	-	chr2.4:3485802-3485821	None:intergenic	30.0%
	AAGAGTTGGAGAAAAATGGT+GGG	+	chr2.4:3486034-3486053	MS.gene85151:CDS	35.0%
	CGTGTACGTGATAAATACTT+AGG	+	chr2.4:3485973-3485992	MS.gene85151:CDS	35.0%
	CTTCAGCTTGAAACAAAACA+GGG	-	chr2.4:3486131-3486150	None:intergenic	35.0%
	GCATCTTCAACTTCAGTTAT+CGG	-	chr2.4:3485901-3485920	None:intergenic	35.0%
!	TTTGGAGCATACAATGTTGT+TGG	-	chr2.4:3485770-3485789	None:intergenic	35.0%
!!	TGAATTAGGTTTTGGTGGTT+TGG	-	chr2.4:3485788-3485807	None:intergenic	35.0%
!!	TGAGATGAATTAGGTTTTGG+TGG	-	chr2.4:3485793-3485812	None:intergenic	35.0%
	AGAGTAAAATGGAAGGGAGA+AGG	+	chr2.4:3486172-3486191	MS.gene85151:CDS	40.0%
	AGAGTTGGAGAAAAATGGTG+GGG	+	chr2.4:3486035-3486054	MS.gene85151:CDS	40.0%
	CATTACCTAGCTCAATCTCT+AGG	+	chr2.4:3485743-3485762	MS.gene85151:CDS	40.0%
	CTAGGTAATGAATCTCCATG+AGG	-	chr2.4:3485733-3485752	None:intergenic	40.0%
	GAAGAGTTGGAGAAAAATGG+TGG	+	chr2.4:3486033-3486052	MS.gene85151:CDS	40.0%
	GAGTAAAATGGAAGGGAGAA+GGG	+	chr2.4:3486173-3486192	MS.gene85151:CDS	40.0%
	GATGCAAAGTGTGAATGTTG+TGG	+	chr2.4:3485916-3485935	MS.gene85151:CDS	40.0%
	GCTTCAGCTTGAAACAAAAC+AGG	-	chr2.4:3486132-3486151	None:intergenic	40.0%
	GTGGAAGCTGTGTTAAATCA+AGG	-	chr2.4:3485879-3485898	None:intergenic	40.0%
	TCATTCAACCCTAGAGACAA+AGG	+	chr2.4:3486201-3486220	MS.gene85151:CDS	40.0%
	TCTTCAACTTCAGTTATCGG+TGG	-	chr2.4:3485898-3485917	None:intergenic	40.0%
	TGGTGGGGAAAATATTGAAG+AGG	+	chr2.4:3486050-3486069	MS.gene85151:CDS	40.0%
	TTTCACCACCTTTGTCTCTA+GGG	-	chr2.4:3486212-3486231	None:intergenic	40.0%
!	CGTACACGTTTTATGTACTC+AGG	-	chr2.4:3485961-3485980	None:intergenic	40.0%
!	TACTTAGGGAAATGGGTTTG+TGG	+	chr2.4:3485988-3486007	MS.gene85151:CDS	40.0%
!	TTTTCACCACCTTTGTCTCT+AGG	-	chr2.4:3486213-3486232	None:intergenic	40.0%
	AAAGGTGGTGAAAAGAGAGG+TGG	+	chr2.4:3486219-3486238	MS.gene85151:CDS	45.0%
	GACAAAGGTGGTGAAAAGAG+AGG	+	chr2.4:3486216-3486235	MS.gene85151:CDS	45.0%
	TGAGGCAGTGAAAGAAGAGT+TGG	+	chr2.4:3486020-3486039	MS.gene85151:CDS	45.0%
	TTCAACCCTAGAGACAAAGG+TGG	+	chr2.4:3486204-3486223	MS.gene85151:CDS	45.0%
	TTGGTCCTAGAGATTGAGCT+AGG	-	chr2.4:3485751-3485770	None:intergenic	45.0%
!	GGTTTGTGGTTTGTGTTCTG+AGG	+	chr2.4:3486002-3486021	MS.gene85151:CDS	45.0%
	GTGGTGAAAAGAGAGGTGGT+GGG	+	chr2.4:3486223-3486242	MS.gene85151:CDS	50.0%
	GGTGGTGAAAAGAGAGGTGG+TGG	+	chr2.4:3486222-3486241	MS.gene85151:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	3485724	3486314	3485724	ID=MS.gene85151
chr2.4	mRNA	3485724	3486314	3485724	ID=MS.gene85151.t1;Parent=MS.gene85151
chr2.4	exon	3485724	3486314	3485724	ID=MS.gene85151.t1.exon1;Parent=MS.gene85151.t1
chr2.4	CDS	3485724	3486314	3485724	ID=cds.MS.gene85151.t1;Parent=MS.gene85151.t1

Gene Sequence

>MS.gene85151

ATGGCACCTCATGGAGATTCATTACCTAGCTCAATCTCTAGGACCAACAACATTGTATGCTCCAAACCACCAAAACCTAATTCATCTCAACTCAACAACAATAATATTCAAAGAACAATCTCAGACATATCATTTGAACTATCAAAACAAGACCTTGATTTAACACAGCTTCCACCGATAACTGAAGTTGAAGATGCAAAGTGTGAATGTTGTGGCATGTCTGAAGAGTTTACACCTGAGTACATAAAACGTGTACGTGATAAATACTTAGGGAAATGGGTTTGTGGTTTGTGTTCTGAGGCAGTGAAAGAAGAGTTGGAGAAAAATGGTGGGGAAAATATTGAAGAGGCTTTGAGTGCACACATGAGTGCATGTGTTAGATTTAATAAATTTGGAAGGAGTTTCCCTGTTTTGTTTCAAGCTGAAGCTATGAAAGAGATGCTGAAAAAGAGTAAAATGGAAGGGAGAAGGGCTAAGTCATTCAACCCTAGAGACAAAGGTGGTGAAAAGAGAGGTGGTGGGCTTGCTAGAAGTTCAAGTTGCATTCCTGCTATTACAAGAGATATCAATGATTTCACAATCACCAATTAA

Protein sequence

>MS.gene85151.t1

MAPHGDSLPSSISRTNNIVCSKPPKPNSSQLNNNNIQRTISDISFELSKQDLDLTQLPPITEVEDAKCECCGMSEEFTPEYIKRVRDKYLGKWVCGLCSEAVKEELEKNGGENIEEALSAHMSACVRFNKFGRSFPVLFQAEAMKEMLKKSKMEGRRAKSFNPRDKGGEKRGGGLARSSSCIPAITRDINDFTITN