Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52997.t1 | XP_003590788.2 | 95.9 | 561 | 23 | 0 | 1 | 561 | 1 | 561 | 3.20E-302 | 1047 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52997.t1 | G7I3Y5 | 95.9 | 561 | 23 | 0 | 1 | 561 | 1 | 561 | 2.3e-302 | 1047.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene52997 | 0.802312 | 5.96E-49 | -1.69E-46 |
| MS.gene049356 | MS.gene52997 | 0.819834 | 9.43E-53 | -1.69E-46 |
| MS.gene049993 | MS.gene52997 | 0.803823 | 2.90E-49 | -1.69E-46 |
| MS.gene050426 | MS.gene52997 | -0.854696 | 1.04E-61 | -1.69E-46 |
| MS.gene050670 | MS.gene52997 | 0.896426 | 3.73E-76 | -1.69E-46 |
| MS.gene050971 | MS.gene52997 | 0.823067 | 1.69E-53 | -1.69E-46 |
| MS.gene05105 | MS.gene52997 | 0.817603 | 3.03E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene52997 | 0.810698 | 1.01E-50 | -1.69E-46 |
| MS.gene051521 | MS.gene52997 | 0.830206 | 3.35E-55 | -1.69E-46 |
| MS.gene051525 | MS.gene52997 | 0.872778 | 2.45E-67 | -1.69E-46 |
| MS.gene051582 | MS.gene52997 | 0.828579 | 8.31E-55 | -1.69E-46 |
| MS.gene051588 | MS.gene52997 | 0.828186 | 1.03E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene52997 | 0.819494 | 1.13E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene52997 | -0.884098 | 2.56E-71 | -1.69E-46 |
| MS.gene052077 | MS.gene52997 | 0.856501 | 3.08E-62 | -1.69E-46 |
| MS.gene052128 | MS.gene52997 | -0.800462 | 1.43E-48 | -1.69E-46 |
| MS.gene052134 | MS.gene52997 | 0.816204 | 6.24E-52 | -1.69E-46 |
| MS.gene052135 | MS.gene52997 | 0.831182 | 1.93E-55 | -1.69E-46 |
| MS.gene052136 | MS.gene52997 | 0.809319 | 2.01E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene52997 | 0.84852 | 5.82E-60 | -1.69E-46 |
| MS.gene052253 | MS.gene52997 | 0.870205 | 1.74E-66 | -1.69E-46 |
| MS.gene052340 | MS.gene52997 | 0.806664 | 7.37E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene52997 | 0.840771 | 7.11E-58 | -1.69E-46 |
| MS.gene052421 | MS.gene52997 | 0.824842 | 6.48E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene52997 | 0.816622 | 5.03E-52 | -1.69E-46 |
| MS.gene052505 | MS.gene52997 | 0.806268 | 8.93E-50 | -1.69E-46 |
| MS.gene052982 | MS.gene52997 | 0.806087 | 9.75E-50 | -1.69E-46 |
| MS.gene053169 | MS.gene52997 | 0.842505 | 2.48E-58 | -1.69E-46 |
| MS.gene05316 | MS.gene52997 | 0.818593 | 1.81E-52 | -1.69E-46 |
| MS.gene053176 | MS.gene52997 | 0.804443 | 2.15E-49 | -1.69E-46 |
| MS.gene053494 | MS.gene52997 | 0.800366 | 1.49E-48 | -1.69E-46 |
| MS.gene053758 | MS.gene52997 | 0.811194 | 7.91E-51 | -1.69E-46 |
| MS.gene053889 | MS.gene52997 | 0.803456 | 3.46E-49 | -1.69E-46 |
| MS.gene053928 | MS.gene52997 | 0.817252 | 3.63E-52 | -1.69E-46 |
| MS.gene054102 | MS.gene52997 | 0.807184 | 5.72E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene52997 | 0.882698 | 8.38E-71 | -1.69E-46 |
| MS.gene054349 | MS.gene52997 | 0.850022 | 2.22E-60 | -1.69E-46 |
| MS.gene054548 | MS.gene52997 | 0.805358 | 1.39E-49 | -1.69E-46 |
| MS.gene054556 | MS.gene52997 | 0.863417 | 2.53E-64 | -1.69E-46 |
| MS.gene054719 | MS.gene52997 | 0.808353 | 3.23E-50 | -1.69E-46 |
| MS.gene054918 | MS.gene52997 | 0.820099 | 8.20E-53 | -1.69E-46 |
| MS.gene054920 | MS.gene52997 | 0.833301 | 5.77E-56 | -1.69E-46 |
| MS.gene055116 | MS.gene52997 | 0.838991 | 2.07E-57 | -1.69E-46 |
| MS.gene055369 | MS.gene52997 | 0.876565 | 1.26E-68 | -1.69E-46 |
| MS.gene055539 | MS.gene52997 | 0.821187 | 4.61E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene52997 | 0.851945 | 6.38E-61 | -1.69E-46 |
| MS.gene05556 | MS.gene52997 | 0.810999 | 8.72E-51 | -1.69E-46 |
| MS.gene055741 | MS.gene52997 | 0.833598 | 4.87E-56 | -1.69E-46 |
| MS.gene055744 | MS.gene52997 | 0.844122 | 9.19E-59 | -1.69E-46 |
| MS.gene055909 | MS.gene52997 | 0.840583 | 7.97E-58 | -1.69E-46 |
| MS.gene055911 | MS.gene52997 | 0.845798 | 3.24E-59 | -1.69E-46 |
| MS.gene05594 | MS.gene52997 | 0.811632 | 6.35E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene52997 | 0.809778 | 1.60E-50 | -1.69E-46 |
| MS.gene056330 | MS.gene52997 | -0.814897 | 1.22E-51 | -1.69E-46 |
| MS.gene056407 | MS.gene52997 | 0.827298 | 1.69E-54 | -1.69E-46 |
| MS.gene056511 | MS.gene52997 | 0.84823 | 7.00E-60 | -1.69E-46 |
| MS.gene056514 | MS.gene52997 | 0.859756 | 3.32E-63 | -1.69E-46 |
| MS.gene056659 | MS.gene52997 | 0.824793 | 6.66E-54 | -1.69E-46 |
| MS.gene056723 | MS.gene52997 | 0.859923 | 2.95E-63 | -1.69E-46 |
| MS.gene056876 | MS.gene52997 | 0.801419 | 9.08E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene52997 | -0.801767 | 7.71E-49 | -1.69E-46 |
| MS.gene056902 | MS.gene52997 | 0.808428 | 3.11E-50 | -1.69E-46 |
| MS.gene057124 | MS.gene52997 | 0.817153 | 3.82E-52 | -1.69E-46 |
| MS.gene057396 | MS.gene52997 | 0.873938 | 9.97E-68 | -1.69E-46 |
| MS.gene057476 | MS.gene52997 | 0.836499 | 9.03E-57 | -1.69E-46 |
| MS.gene057609 | MS.gene52997 | 0.813446 | 2.55E-51 | -1.69E-46 |
| MS.gene057610 | MS.gene52997 | 0.827501 | 1.51E-54 | -1.69E-46 |
| MS.gene057749 | MS.gene52997 | 0.832139 | 1.12E-55 | -1.69E-46 |
| MS.gene057823 | MS.gene52997 | 0.844393 | 7.77E-59 | -1.69E-46 |
| MS.gene057825 | MS.gene52997 | 0.80624 | 9.06E-50 | -1.69E-46 |
| MS.gene057888 | MS.gene52997 | 0.828247 | 9.99E-55 | -1.69E-46 |
| MS.gene058007 | MS.gene52997 | 0.858326 | 8.90E-63 | -1.69E-46 |
| MS.gene058072 | MS.gene52997 | 0.811214 | 7.83E-51 | -1.69E-46 |
| MS.gene058075 | MS.gene52997 | 0.840594 | 7.92E-58 | -1.69E-46 |
| MS.gene058120 | MS.gene52997 | 0.816355 | 5.77E-52 | -1.69E-46 |
| MS.gene058376 | MS.gene52997 | 0.821986 | 3.02E-53 | -1.69E-46 |
| MS.gene058444 | MS.gene52997 | 0.813911 | 2.01E-51 | -1.69E-46 |
| MS.gene058651 | MS.gene52997 | 0.80462 | 1.98E-49 | -1.69E-46 |
| MS.gene058700 | MS.gene52997 | 0.853447 | 2.38E-61 | -1.69E-46 |
| MS.gene058710 | MS.gene52997 | 0.810703 | 1.01E-50 | -1.69E-46 |
| MS.gene05886 | MS.gene52997 | 0.817813 | 2.71E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene52997 | 0.80638 | 8.46E-50 | -1.69E-46 |
| MS.gene058948 | MS.gene52997 | 0.803087 | 4.12E-49 | -1.69E-46 |
| MS.gene058949 | MS.gene52997 | 0.817002 | 4.13E-52 | -1.69E-46 |
| MS.gene058995 | MS.gene52997 | 0.809756 | 1.62E-50 | -1.69E-46 |
| MS.gene059011 | MS.gene52997 | 0.842797 | 2.08E-58 | -1.69E-46 |
| MS.gene05907 | MS.gene52997 | 0.824368 | 8.38E-54 | -1.69E-46 |
| MS.gene059082 | MS.gene52997 | 0.845183 | 4.76E-59 | -1.69E-46 |
| MS.gene059141 | MS.gene52997 | 0.812141 | 4.92E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene52997 | 0.812346 | 4.44E-51 | -1.69E-46 |
| MS.gene059366 | MS.gene52997 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene52997 | 0.902483 | 9.25E-79 | -1.69E-46 |
| MS.gene059462 | MS.gene52997 | 0.852311 | 5.02E-61 | -1.69E-46 |
| MS.gene059674 | MS.gene52997 | 0.800306 | 1.53E-48 | -1.69E-46 |
| MS.gene059688 | MS.gene52997 | 0.827748 | 1.32E-54 | -1.69E-46 |
| MS.gene059692 | MS.gene52997 | 0.801908 | 7.21E-49 | -1.69E-46 |
| MS.gene059695 | MS.gene52997 | 0.804225 | 2.39E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene52997 | 0.875163 | 3.83E-68 | -1.69E-46 |
| MS.gene059868 | MS.gene52997 | 0.847519 | 1.10E-59 | -1.69E-46 |
| MS.gene059870 | MS.gene52997 | 0.808779 | 2.62E-50 | -1.69E-46 |
| MS.gene059876 | MS.gene52997 | 0.863114 | 3.14E-64 | -1.69E-46 |
| MS.gene059933 | MS.gene52997 | 0.839472 | 1.55E-57 | -1.69E-46 |
| MS.gene059958 | MS.gene52997 | 0.843888 | 1.06E-58 | -1.69E-46 |
| MS.gene060247 | MS.gene52997 | 0.829557 | 4.81E-55 | -1.69E-46 |
| MS.gene060372 | MS.gene52997 | 0.830049 | 3.65E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene52997 | 0.838294 | 3.13E-57 | -1.69E-46 |
| MS.gene060837 | MS.gene52997 | 0.820945 | 5.25E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene52997 | 0.811866 | 5.65E-51 | -1.69E-46 |
| MS.gene061154 | MS.gene52997 | 0.871788 | 5.23E-67 | -1.69E-46 |
| MS.gene061159 | MS.gene52997 | 0.818878 | 1.56E-52 | -1.69E-46 |
| MS.gene061214 | MS.gene52997 | 0.83558 | 1.55E-56 | -1.69E-46 |
| MS.gene061215 | MS.gene52997 | 0.835126 | 2.01E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52997.t1 | MTR_1g075280 | 95.900 | 561 | 23 | 0 | 1 | 561 | 1 | 561 | 0.0 | 1118 |
| MS.gene52997.t1 | MTR_1g075280 | 88.948 | 561 | 23 | 1 | 1 | 561 | 1 | 522 | 0.0 | 1019 |
| MS.gene52997.t1 | MTR_5g007040 | 34.983 | 303 | 134 | 8 | 207 | 462 | 94 | 380 | 3.24e-32 | 132 |
| MS.gene52997.t1 | MTR_5g007040 | 49.038 | 104 | 47 | 1 | 1 | 104 | 9 | 106 | 2.09e-22 | 102 |
| MS.gene52997.t1 | MTR_6g046940 | 28.502 | 414 | 203 | 16 | 207 | 556 | 94 | 478 | 1.13e-21 | 99.8 |
| MS.gene52997.t1 | MTR_6g046940 | 46.154 | 104 | 50 | 1 | 1 | 104 | 9 | 106 | 1.12e-19 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 90 sgRNAs with CRISPR-Local
Find 149 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAATCTTCTACTATTTC+AGG | 0.180626 | 1.1:-56374309 | MS.gene52997:intron |
| ACAATATGTGGACTGAAAAT+TGG | 0.244513 | 1.1:+56373208 | None:intergenic |
| GAATCTCTCAATCTCGAAAA+AGG | 0.270101 | 1.1:+56372785 | None:intergenic |
| GCCAGGATCTGGTGATAATT+TGG | 0.270512 | 1.1:-56374475 | MS.gene52997:intron |
| TTAAGACTGAAACCTTTATC+TGG | 0.299755 | 1.1:+56375027 | None:intergenic |
| ATGACTTCAAAACCAGTCTT+TGG | 0.306670 | 1.1:+56375177 | None:intergenic |
| CCTGTATCATGTTCTTGATA+TGG | 0.316401 | 1.1:+56374772 | None:intergenic |
| ATCCTCTATCTGAAATAGTT+TGG | 0.324431 | 1.1:-56375053 | MS.gene52997:CDS |
| AGCTAAAATATCTGAGATAA+TGG | 0.328696 | 1.1:-56374340 | MS.gene52997:CDS |
| TCTTGATATGGTGTATATTC+TGG | 0.346161 | 1.1:+56374784 | None:intergenic |
| TGTTTGTTGGCATTGTTTGA+TGG | 0.368003 | 1.1:+56372724 | None:intergenic |
| TTGATGAGACATTCTTGTTC+CGG | 0.369355 | 1.1:+56373169 | None:intergenic |
| CCTTCTTTGTAATGTTTCCT+TGG | 0.372511 | 1.1:+56373827 | None:intergenic |
| ATAGTTTGGACTCCAGATAA+AGG | 0.389342 | 1.1:-56375039 | MS.gene52997:CDS |
| TGCTGGAGGAACATCTGATT+TGG | 0.391359 | 1.1:-56374439 | MS.gene52997:CDS |
| TTTGAAAACTATCATAATCC+TGG | 0.393605 | 1.1:-56373503 | MS.gene52997:CDS |
| GTAAATTACCAACTGGACTT+TGG | 0.394116 | 1.1:+56373897 | None:intergenic |
| TCAAGATGAGGAGAAGTCTT+TGG | 0.394857 | 1.1:-56373312 | MS.gene52997:CDS |
| TTGGAGAAAATTAACTCTGC+TGG | 0.405206 | 1.1:-56374456 | MS.gene52997:CDS |
| TGACTTTCATTCTTATTGTA+TGG | 0.410801 | 1.1:+56373533 | None:intergenic |
| TATTTCAGATAGAGGATCAT+TGG | 0.411235 | 1.1:+56375059 | None:intergenic |
| GAACACATGATTGAAGATAA+AGG | 0.411777 | 1.1:-56373743 | MS.gene52997:CDS |
| TGGTAGACTATTCTGTTTGT+TGG | 0.430093 | 1.1:+56372711 | None:intergenic |
| TCCATGTTTGCAAAGAGGTT+AGG | 0.431116 | 1.1:-56372749 | MS.gene52997:CDS |
| CGATGTTGAACCTAGTAGTA+TGG | 0.433354 | 1.1:-56374959 | MS.gene52997:CDS |
| TCATAGCTCATTCATGAACT+TGG | 0.436526 | 1.1:-56373369 | MS.gene52997:CDS |
| GGAGAATTTAATGAACCATT+GGG | 0.439177 | 1.1:-56374403 | MS.gene52997:CDS |
| GGCCATCGAAGGTGGCGATC+AGG | 0.439496 | 1.1:+56373272 | None:intergenic |
| TGAACTTGTAAATTACCAAC+TGG | 0.441384 | 1.1:+56373890 | None:intergenic |
| GTGTTCTTCTTTACATCCTC+AGG | 0.443827 | 1.1:-56373111 | MS.gene52997:intron |
| GTCCAAACTATTTCAGATAG+AGG | 0.446372 | 1.1:+56375051 | None:intergenic |
| AGCTGTTTCAATTTCAATGC+AGG | 0.446482 | 1.1:+56374359 | None:intergenic |
| AGGAGAATTTAATGAACCAT+TGG | 0.446595 | 1.1:-56374404 | MS.gene52997:CDS |
| ATCACCCGGCATTAATCATT+CGG | 0.448847 | 1.1:-56373693 | MS.gene52997:CDS |
| CTTCTCATCTTTAAGACCAA+TGG | 0.453090 | 1.1:+56372853 | None:intergenic |
| TTGTAATGTTTCCTTGGTGA+AGG | 0.453555 | 1.1:+56373833 | None:intergenic |
| AAAGAGGCTTCTTCTTCCAT+TGG | 0.453603 | 1.1:-56372869 | MS.gene52997:CDS |
| CCACCTTCGATGGCCTTACA+AGG | 0.455789 | 1.1:-56373264 | MS.gene52997:CDS |
| AATTTACAAGTTCATCAAGA+TGG | 0.458602 | 1.1:-56373881 | MS.gene52997:CDS |
| CCAAGGAAACATTACAAAGA+AGG | 0.463419 | 1.1:-56373827 | MS.gene52997:CDS |
| TTGGTTTCAAACATGACTAA+AGG | 0.464068 | 1.1:-56373350 | MS.gene52997:CDS |
| GGAACATCTGATTTGGCAAA+TGG | 0.468310 | 1.1:-56374432 | MS.gene52997:CDS |
| GGACTGAAAATTGGATTTGA+AGG | 0.473417 | 1.1:+56373217 | None:intergenic |
| TCTGGAGCTTGACCCTTGTA+AGG | 0.474736 | 1.1:+56373251 | None:intergenic |
| TGCACTAAAACGTCTTCTGC+AGG | 0.475147 | 1.1:+56374895 | None:intergenic |
| ACAATATGCTGATAACCAAT+TGG | 0.476134 | 1.1:-56372930 | MS.gene52997:CDS |
| TGATTTGGCAAATGGTCAAA+AGG | 0.486780 | 1.1:-56374424 | MS.gene52997:CDS |
| AAGAGTGACGTTTCTTCGAC+AGG | 0.500026 | 1.1:-56374850 | MS.gene52997:CDS |
| GAAACATTACAAAGAAGGCT+TGG | 0.503191 | 1.1:-56373822 | MS.gene52997:CDS |
| CTCAAAACACTGACAATATG+TGG | 0.503790 | 1.1:+56373196 | None:intergenic |
| CTTCGACAGGTACTCCTGCT+AGG | 0.511003 | 1.1:-56374837 | MS.gene52997:CDS |
| CACAAATATTCTTCTCCCAA+TGG | 0.514033 | 1.1:+56374388 | None:intergenic |
| AATATCTGAGATAATGGAAG+AGG | 0.514653 | 1.1:-56374334 | MS.gene52997:CDS |
| GATAAAGGTTCTAATCCTCT+AGG | 0.516529 | 1.1:-56373728 | MS.gene52997:CDS |
| ATTGAGAGATTCAGAGGCAA+TGG | 0.519119 | 1.1:-56372774 | MS.gene52997:CDS |
| CATTGGTCTTAAAGATGAGA+AGG | 0.529065 | 1.1:-56372852 | MS.gene52997:CDS |
| TGGTGTATATTCTGGAAAGA+TGG | 0.529676 | 1.1:+56374792 | None:intergenic |
| ATTTACAAGTTCATCAAGAT+GGG | 0.530667 | 1.1:-56373880 | MS.gene52997:CDS |
| TTATTCTAGTCAATGTCATA+AGG | 0.530823 | 1.1:-56375148 | MS.gene52997:CDS |
| AAGAAAATGAAACCAAAGAC+TGG | 0.532411 | 1.1:-56375189 | None:intergenic |
| TCGCTTGTTGAATTCCTAGC+AGG | 0.535881 | 1.1:+56374823 | None:intergenic |
| TAGCATCATCTCATTGACCT+TGG | 0.536137 | 1.1:+56373556 | None:intergenic |
| CCATATCAAGAACATGATAC+AGG | 0.536521 | 1.1:-56374772 | MS.gene52997:intron |
| ATCCTGATCGCCACCTTCGA+TGG | 0.538722 | 1.1:-56373274 | MS.gene52997:CDS |
| ACCTAACCTCTTTGCAAACA+TGG | 0.543122 | 1.1:+56372748 | None:intergenic |
| TTCGAGATTGAGAGATTCAG+AGG | 0.550691 | 1.1:-56372780 | MS.gene52997:CDS |
| AGAGCAAGAACCATACTACT+AGG | 0.554225 | 1.1:+56374949 | None:intergenic |
| AGATGGGTCTAAACTTAGCA+TGG | 0.563633 | 1.1:-56373864 | MS.gene52997:CDS |
| TTGCAAACTGAGTCAAGTGC+AGG | 0.567039 | 1.1:-56375120 | MS.gene52997:CDS |
| TTCAACATCGCGCAAAAGCG+AGG | 0.569210 | 1.1:+56374972 | None:intergenic |
| TGGTTTCCATGTTTGCAAAG+AGG | 0.574375 | 1.1:-56372754 | MS.gene52997:CDS |
| ACAAGAATGTCTCATCAAGT+AGG | 0.578856 | 1.1:-56373164 | MS.gene52997:CDS |
| TGAGCTATGATGATTAACAT+AGG | 0.579724 | 1.1:+56373382 | None:intergenic |
| GACCCTTGTAAGGCCATCGA+AGG | 0.580322 | 1.1:+56373261 | None:intergenic |
| TTTGCGCATATAACAGCAAG+AGG | 0.582464 | 1.1:+56374871 | None:intergenic |
| ATTGGTCTTAAAGATGAGAA+GGG | 0.585207 | 1.1:-56372851 | MS.gene52997:CDS |
| CCTTGTAAGGCCATCGAAGG+TGG | 0.590280 | 1.1:+56373264 | None:intergenic |
| GAGAAAATTAACTCTGCTGG+AGG | 0.604426 | 1.1:-56374453 | MS.gene52997:CDS |
| CAGATCAACCAAAGTCCAGT+TGG | 0.604525 | 1.1:-56373905 | MS.gene52997:CDS |
| GTCTTCTGCAGGTTTATCTG+TGG | 0.625174 | 1.1:+56374906 | None:intergenic |
| GGATACTAGTGTTGACAACA+TGG | 0.626835 | 1.1:-56373801 | MS.gene52997:CDS |
| GGAACATATTTGATATCACC+CGG | 0.627350 | 1.1:-56373707 | MS.gene52997:CDS |
| AAGAAGAACACCGTCTGCAG+AGG | 0.634486 | 1.1:+56373123 | None:intergenic |
| CAAGCAATAGCCTCTGCAGA+CGG | 0.634882 | 1.1:-56373133 | MS.gene52997:CDS |
| AGATGAGAAGGGAAACAATG+AGG | 0.641689 | 1.1:-56372840 | MS.gene52997:CDS |
| CTCACAATCTAGTCAAGATG+AGG | 0.644826 | 1.1:-56373324 | MS.gene52997:CDS |
| CACCTTCGATGGCCTTACAA+GGG | 0.654668 | 1.1:-56373263 | MS.gene52997:CDS |
| GATATCAAATATGTTCCTAG+AGG | 0.673634 | 1.1:+56373713 | None:intergenic |
| TGGAACAAAATCCTTCACCA+AGG | 0.714111 | 1.1:-56373844 | MS.gene52997:CDS |
| TACTAGTGTTGACAACATGG+TGG | 0.817206 | 1.1:-56373798 | MS.gene52997:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCATGATAGTATCTTAAAA+TGG | + | chr1.1:56373655-56373674 | None:intergenic | 20.0% |
| !! | TAAAAATTAATAACTTCAGC+AGG | + | chr1.1:56373908-56373927 | None:intergenic | 20.0% |
| !! | TTTACAAAATAGAATCAGTA+AGG | - | chr1.1:56374878-56374897 | MS.gene52997:CDS | 20.0% |
| !!! | AAGGTTTTAAAGGAAAAAAT+TGG | + | chr1.1:56373381-56373400 | None:intergenic | 20.0% |
| !!! | ATATAAATTTTCCTCAATCA+TGG | - | chr1.1:56373717-56373736 | MS.gene52997:CDS | 20.0% |
| !!! | TTTAAGATACTATCATGATT+TGG | - | chr1.1:56373656-56373675 | MS.gene52997:CDS | 20.0% |
| ! | AACAAATCTTCTACTATTTC+AGG | - | chr1.1:56373592-56373611 | MS.gene52997:CDS | 25.0% |
| ! | AATTTACAAGTTCATCAAGA+TGG | - | chr1.1:56374020-56374039 | MS.gene52997:intron | 25.0% |
| ! | ATATCATGATTGAGCAAAAA+AGG | + | chr1.1:56373400-56373419 | None:intergenic | 25.0% |
| ! | ATTAATTAAAGTGACACTAG+TGG | + | chr1.1:56373341-56373360 | None:intergenic | 25.0% |
| ! | ATTTACAAGTTCATCAAGAT+GGG | - | chr1.1:56374021-56374040 | MS.gene52997:intron | 25.0% |
| ! | CAGTAATTCAAATGAGAAAT+TGG | + | chr1.1:56374930-56374949 | None:intergenic | 25.0% |
| ! | CATTAATCATTCGGAAAAAA+TGG | - | chr1.1:56374217-56374236 | MS.gene52997:intron | 25.0% |
| ! | CGATAATAAAAACAAATCCA+AGG | - | chr1.1:56374328-56374347 | MS.gene52997:CDS | 25.0% |
| ! | TGACTTTCATTCTTATTGTA+TGG | + | chr1.1:56374371-56374390 | None:intergenic | 25.0% |
| ! | TGGCTATTGAGAAAATTTAT+AGG | + | chr1.1:56374821-56374840 | None:intergenic | 25.0% |
| ! | TTATTCTAGTCAATGTCATA+AGG | - | chr1.1:56372753-56372772 | MS.gene52997:CDS | 25.0% |
| ! | TTTAGATATGCAAAAGAGAA+TGG | + | chr1.1:56373946-56373965 | None:intergenic | 25.0% |
| ! | TTTGAAAACTATCATAATCC+TGG | - | chr1.1:56374398-56374417 | MS.gene52997:CDS | 25.0% |
| !! | AGCTAAAATATCTGAGATAA+TGG | - | chr1.1:56373561-56373580 | MS.gene52997:CDS | 25.0% |
| !! | TTGAAGGAATTTTTAATCTC+TGG | + | chr1.1:56374671-56374690 | None:intergenic | 25.0% |
| !!! | GAATTGTTTTATCAACAAAG+AGG | - | chr1.1:56375016-56375035 | MS.gene52997:CDS | 25.0% |
| !!! | GGATTTGTTTTTATTATCGT+TGG | + | chr1.1:56374327-56374346 | None:intergenic | 25.0% |
| !!! | TTGAGCAAAAAAGGTTTTAA+AGG | + | chr1.1:56373391-56373410 | None:intergenic | 25.0% |
| ACAATATGCTGATAACCAAT+TGG | - | chr1.1:56374971-56374990 | MS.gene52997:CDS | 30.0% | |
| AGGAGAATTTAATGAACCAT+TGG | - | chr1.1:56373497-56373516 | MS.gene52997:CDS | 30.0% | |
| CAATTAACACTGCATATCAA+AGG | - | chr1.1:56373750-56373769 | MS.gene52997:CDS | 30.0% | |
| CTTTGATATGCAGTGTTAAT+TGG | + | chr1.1:56373752-56373771 | None:intergenic | 30.0% | |
| GAACACATGATTGAAGATAA+AGG | - | chr1.1:56374158-56374177 | MS.gene52997:intron | 30.0% | |
| GATATCAAATATGTTCCTAG+AGG | + | chr1.1:56374191-56374210 | None:intergenic | 30.0% | |
| GGAGAATTTAATGAACCATT+GGG | - | chr1.1:56373498-56373517 | MS.gene52997:CDS | 30.0% | |
| GGTAAATATAACATCCCTTA+TGG | - | chr1.1:56373150-56373169 | MS.gene52997:CDS | 30.0% | |
| TATTTCAGATAGAGGATCAT+TGG | + | chr1.1:56372845-56372864 | None:intergenic | 30.0% | |
| TCTTGATATGGTGTATATTC+TGG | + | chr1.1:56373120-56373139 | None:intergenic | 30.0% | |
| TGAACTTGTAAATTACCAAC+TGG | + | chr1.1:56374014-56374033 | None:intergenic | 30.0% | |
| TGAGCTATGATGATTAACAT+AGG | + | chr1.1:56374522-56374541 | None:intergenic | 30.0% | |
| TTAAGACTGAAACCTTTATC+TGG | + | chr1.1:56372877-56372896 | None:intergenic | 30.0% | |
| TTCTCATTTGAATTACTGCA+AGG | - | chr1.1:56374932-56374951 | MS.gene52997:CDS | 30.0% | |
| TTGGTTTCAAACATGACTAA+AGG | - | chr1.1:56374551-56374570 | MS.gene52997:intron | 30.0% | |
| ! | ACAATATGTGGACTGAAAAT+TGG | + | chr1.1:56374696-56374715 | None:intergenic | 30.0% |
| ! | ATCCTCTATCTGAAATAGTT+TGG | - | chr1.1:56372848-56372867 | MS.gene52997:CDS | 30.0% |
| ! | TCATAATCCTGGATTGTTTT+TGG | - | chr1.1:56374409-56374428 | MS.gene52997:CDS | 30.0% |
| ! | TGCTGTCATAATTGATTCTT+TGG | - | chr1.1:56373619-56373638 | MS.gene52997:CDS | 30.0% |
| ! | TTTTAGTCTCAATGACCAAT+TGG | + | chr1.1:56374989-56375008 | None:intergenic | 30.0% |
| ! | TTTTTCCGAATGATTAATGC+CGG | + | chr1.1:56374216-56374235 | None:intergenic | 30.0% |
| !! | AATATCTGAGATAATGGAAG+AGG | - | chr1.1:56373567-56373586 | MS.gene52997:CDS | 30.0% |
| !! | ATTGGTCTTAAAGATGAGAA+GGG | - | chr1.1:56375050-56375069 | MS.gene52997:CDS | 30.0% |
| !! | GGATTTATTTTCCATGATTG+AGG | + | chr1.1:56373731-56373750 | None:intergenic | 30.0% |
| !! | GTGATATTTTTCAGTGTTCA+TGG | + | chr1.1:56374841-56374860 | None:intergenic | 30.0% |
| AAATTTATAGGCCTAACCTG+AGG | + | chr1.1:56374809-56374828 | None:intergenic | 35.0% | |
| AATCATGATATTGATCTGCC+AGG | - | chr1.1:56373409-56373428 | MS.gene52997:CDS | 35.0% | |
| ACAAGAATGTCTCATCAAGT+AGG | - | chr1.1:56374737-56374756 | MS.gene52997:intron | 35.0% | |
| ATAGTTTGGACTCCAGATAA+AGG | - | chr1.1:56372862-56372881 | MS.gene52997:CDS | 35.0% | |
| ATGACTTCAAAACCAGTCTT+TGG | + | chr1.1:56372727-56372746 | None:intergenic | 35.0% | |
| CACAAATATTCTTCTCCCAA+TGG | + | chr1.1:56373516-56373535 | None:intergenic | 35.0% | |
| CCAAGGAAACATTACAAAGA+AGG | - | chr1.1:56374074-56374093 | MS.gene52997:intron | 35.0% | |
| CCATATCAAGAACATGATAC+AGG | - | chr1.1:56373129-56373148 | MS.gene52997:CDS | 35.0% | |
| CCTGTATCATGTTCTTGATA+TGG | + | chr1.1:56373132-56373151 | None:intergenic | 35.0% | |
| CCTTCTTTGTAATGTTTCCT+TGG | + | chr1.1:56374077-56374096 | None:intergenic | 35.0% | |
| CTCAAAACACTGACAATATG+TGG | + | chr1.1:56374708-56374727 | None:intergenic | 35.0% | |
| GAAACATTACAAAGAAGGCT+TGG | - | chr1.1:56374079-56374098 | MS.gene52997:intron | 35.0% | |
| GAATCTCTCAATCTCGAAAA+AGG | + | chr1.1:56375119-56375138 | None:intergenic | 35.0% | |
| GATTTGAATTTCATCCCTTC+AGG | + | chr1.1:56373295-56373314 | None:intergenic | 35.0% | |
| GGAACATATTTGATATCACC+CGG | - | chr1.1:56374194-56374213 | MS.gene52997:intron | 35.0% | |
| GGACTGAAAATTGGATTTGA+AGG | + | chr1.1:56374687-56374706 | None:intergenic | 35.0% | |
| GTAAATTACCAACTGGACTT+TGG | + | chr1.1:56374007-56374026 | None:intergenic | 35.0% | |
| GTCCAAACTATTTCAGATAG+AGG | + | chr1.1:56372853-56372872 | None:intergenic | 35.0% | |
| TACATCTGTAGGATCCATAA+GGG | + | chr1.1:56373167-56373186 | None:intergenic | 35.0% | |
| TCATAGCTCATTCATGAACT+TGG | - | chr1.1:56374532-56374551 | MS.gene52997:intron | 35.0% | |
| TGAGTTTGCAGTAAGAACTT+TGG | - | chr1.1:56373195-56373214 | MS.gene52997:CDS | 35.0% | |
| TTATGGATCCTACAGATGTA+GGG | - | chr1.1:56373167-56373186 | MS.gene52997:CDS | 35.0% | |
| TTGATGAGACATTCTTGTTC+CGG | + | chr1.1:56374735-56374754 | None:intergenic | 35.0% | |
| TTGGAGAAAATTAACTCTGC+TGG | - | chr1.1:56373445-56373464 | MS.gene52997:CDS | 35.0% | |
| TTGTAATGTTTCCTTGGTGA+AGG | + | chr1.1:56374071-56374090 | None:intergenic | 35.0% | |
| ! | AGCTGTTTCAATTTCAATGC+AGG | + | chr1.1:56373545-56373564 | None:intergenic | 35.0% |
| ! | GAAGTATTTACTTTGTCACG+AGG | - | chr1.1:56373236-56373255 | MS.gene52997:CDS | 35.0% |
| ! | GATAAAGGTTCTAATCCTCT+AGG | - | chr1.1:56374173-56374192 | MS.gene52997:intron | 35.0% |
| ! | TGATTTGGCAAATGGTCAAA+AGG | - | chr1.1:56373477-56373496 | MS.gene52997:CDS | 35.0% |
| ! | TGGTGTATATTCTGGAAAGA+TGG | + | chr1.1:56373112-56373131 | None:intergenic | 35.0% |
| ! | TGTTTGTTGGCATTGTTTGA+TGG | + | chr1.1:56375180-56375199 | None:intergenic | 35.0% |
| ! | TTTTCCGAATGATTAATGCC+GGG | + | chr1.1:56374215-56374234 | None:intergenic | 35.0% |
| !! | CATTGGTCTTAAAGATGAGA+AGG | - | chr1.1:56375049-56375068 | MS.gene52997:CDS | 35.0% |
| !! | CTTCTCATCTTTAAGACCAA+TGG | + | chr1.1:56375051-56375070 | None:intergenic | 35.0% |
| AAAGAGGCTTCTTCTTCCAT+TGG | - | chr1.1:56375032-56375051 | MS.gene52997:CDS | 40.0% | |
| ACCTAACCTCTTTGCAAACA+TGG | + | chr1.1:56375156-56375175 | None:intergenic | 40.0% | |
| AGAATGCATTGTCGCATAAC+CGG | + | chr1.1:56373880-56373899 | None:intergenic | 40.0% | |
| AGAGCAAGAACCATACTACT+AGG | + | chr1.1:56372955-56372974 | None:intergenic | 40.0% | |
| AGATGAGAAGGGAAACAATG+AGG | - | chr1.1:56375061-56375080 | MS.gene52997:CDS | 40.0% | |
| AGATGGGTCTAAACTTAGCA+TGG | - | chr1.1:56374037-56374056 | MS.gene52997:intron | 40.0% | |
| ATCACCCGGCATTAATCATT+CGG | - | chr1.1:56374208-56374227 | MS.gene52997:intron | 40.0% | |
| ATTGAGAGATTCAGAGGCAA+TGG | - | chr1.1:56375127-56375146 | MS.gene52997:CDS | 40.0% | |
| CGATGTTGAACCTAGTAGTA+TGG | - | chr1.1:56372942-56372961 | MS.gene52997:CDS | 40.0% | |
| CTACATCTGTAGGATCCATA+AGG | + | chr1.1:56373168-56373187 | None:intergenic | 40.0% | |
| CTACTTGCCAAAAACAATCC+AGG | + | chr1.1:56374419-56374438 | None:intergenic | 40.0% | |
| CTCACAATCTAGTCAAGATG+AGG | - | chr1.1:56374577-56374596 | MS.gene52997:intron | 40.0% | |
| CTTATGGATCCTACAGATGT+AGG | - | chr1.1:56373166-56373185 | MS.gene52997:CDS | 40.0% | |
| CTTCTTTACATCCTCAGGTT+AGG | - | chr1.1:56374795-56374814 | MS.gene52997:CDS | 40.0% | |
| GAGAAAATTAACTCTGCTGG+AGG | - | chr1.1:56373448-56373467 | MS.gene52997:CDS | 40.0% | |
| GATGAAATTCAAATCCCTCG+GGG | - | chr1.1:56373300-56373319 | MS.gene52997:CDS | 40.0% | |
| GGATGAAATTCAAATCCCTC+GGG | - | chr1.1:56373299-56373318 | MS.gene52997:CDS | 40.0% | |
| GGGATGAAATTCAAATCCCT+CGG | - | chr1.1:56373298-56373317 | MS.gene52997:CDS | 40.0% | |
| GTGTTCTTCTTTACATCCTC+AGG | - | chr1.1:56374790-56374809 | MS.gene52997:CDS | 40.0% | |
| TAGCATCATCTCATTGACCT+TGG | + | chr1.1:56374348-56374367 | None:intergenic | 40.0% | |
| TCAAGATGAGGAGAAGTCTT+TGG | - | chr1.1:56374589-56374608 | MS.gene52997:intron | 40.0% | |
| TCACATTTCCCTACATCTGT+AGG | + | chr1.1:56373178-56373197 | None:intergenic | 40.0% | |
| TCAGCTCAATGGTAAGAACT+TGG | - | chr1.1:56373261-56373280 | MS.gene52997:CDS | 40.0% | |
| TCCAAATTATCACCAGATCC+TGG | + | chr1.1:56373430-56373449 | None:intergenic | 40.0% | |
| TCCATGTTTGCAAAGAGGTT+AGG | - | chr1.1:56375152-56375171 | MS.gene52997:CDS | 40.0% | |
| TCTGCATCATGACTTTCTTC+AGG | - | chr1.1:56373972-56373991 | MS.gene52997:intron | 40.0% | |
| TGGAACAAAATCCTTCACCA+AGG | - | chr1.1:56374057-56374076 | MS.gene52997:intron | 40.0% | |
| TGGTTTCCATGTTTGCAAAG+AGG | - | chr1.1:56375147-56375166 | MS.gene52997:CDS | 40.0% | |
| TTCGAGATTGAGAGATTCAG+AGG | - | chr1.1:56375121-56375140 | MS.gene52997:CDS | 40.0% | |
| TTTGCGCATATAACAGCAAG+AGG | + | chr1.1:56373033-56373052 | None:intergenic | 40.0% | |
| ! | CTGCATATCAAAGGCATGTA+CGG | - | chr1.1:56373759-56373778 | MS.gene52997:CDS | 40.0% |
| ! | TTTCATCCCTTCAGGTTACA+CGG | + | chr1.1:56373287-56373306 | None:intergenic | 40.0% |
| !! | GGAACATCTGATTTGGCAAA+TGG | - | chr1.1:56373469-56373488 | MS.gene52997:CDS | 40.0% |
| !! | GGATACTAGTGTTGACAACA+TGG | - | chr1.1:56374100-56374119 | MS.gene52997:intron | 40.0% |
| !! | TACTAGTGTTGACAACATGG+TGG | - | chr1.1:56374103-56374122 | MS.gene52997:intron | 40.0% |
| !!! | CTGGATTGTTTTTGGCAAGT+AGG | - | chr1.1:56374417-56374436 | MS.gene52997:CDS | 40.0% |
| !!! | TGTCAGTGTTTTGAGAATGC+CGG | - | chr1.1:56374713-56374732 | MS.gene52997:intron | 40.0% |
| AAATTCAAATCCCTCGGGGA+CGG | - | chr1.1:56373304-56373323 | MS.gene52997:CDS | 45.0% | |
| AAGAGTGACGTTTCTTCGAC+AGG | - | chr1.1:56373051-56373070 | MS.gene52997:intron | 45.0% | |
| CAGATCAACCAAAGTCCAGT+TGG | - | chr1.1:56373996-56374015 | MS.gene52997:intron | 45.0% | |
| GATATTGATCTGCCAGGATC+TGG | - | chr1.1:56373415-56373434 | MS.gene52997:CDS | 45.0% | |
| GCCAGGATCTGGTGATAATT+TGG | - | chr1.1:56373426-56373445 | MS.gene52997:CDS | 45.0% | |
| GTAACCACATGTGTCAGTGT+CGG | + | chr1.1:56373801-56373820 | None:intergenic | 45.0% | |
| GTCTTCTGCAGGTTTATCTG+TGG | + | chr1.1:56372998-56373017 | None:intergenic | 45.0% | |
| TGCACTAAAACGTCTTCTGC+AGG | + | chr1.1:56373009-56373028 | None:intergenic | 45.0% | |
| TGCTGGAGGAACATCTGATT+TGG | - | chr1.1:56373462-56373481 | MS.gene52997:CDS | 45.0% | |
| TTGCAAACTGAGTCAAGTGC+AGG | - | chr1.1:56372781-56372800 | MS.gene52997:CDS | 45.0% | |
| ! | TCGCTTGTTGAATTCCTAGC+AGG | + | chr1.1:56373081-56373100 | None:intergenic | 45.0% |
| AACTTGGCCGTGTAACCTGA+AGG | - | chr1.1:56373277-56373296 | MS.gene52997:CDS | 50.0% | |
| AAGAAGAACACCGTCTGCAG+AGG | + | chr1.1:56374781-56374800 | None:intergenic | 50.0% | |
| ACTTGGCCGTGTAACCTGAA+GGG | - | chr1.1:56373278-56373297 | MS.gene52997:CDS | 50.0% | |
| CAAGCAATAGCCTCTGCAGA+CGG | - | chr1.1:56374768-56374787 | MS.gene52997:intron | 50.0% | |
| CTACATGTCAGTGTCGTGTC+CGG | - | chr1.1:56373858-56373877 | MS.gene52997:CDS | 50.0% | |
| GTATCCGACACTGACACATG+TGG | - | chr1.1:56373794-56373813 | MS.gene52997:CDS | 50.0% | |
| TTCAACATCGCGCAAAAGCG+AGG | + | chr1.1:56372932-56372951 | None:intergenic | 50.0% | |
| ! | CACCTTCGATGGCCTTACAA+GGG | - | chr1.1:56374638-56374657 | MS.gene52997:intron | 50.0% |
| ! | TCTGGAGCTTGACCCTTGTA+AGG | + | chr1.1:56374653-56374672 | None:intergenic | 50.0% |
| !! | CATTTTTCAACCGTCCCCGA+GGG | + | chr1.1:56373317-56373336 | None:intergenic | 50.0% |
| !! | TCATTTTTCAACCGTCCCCG+AGG | + | chr1.1:56373318-56373337 | None:intergenic | 50.0% |
| ATCCTGATCGCCACCTTCGA+TGG | - | chr1.1:56374627-56374646 | MS.gene52997:intron | 55.0% | |
| CCTTGTAAGGCCATCGAAGG+TGG | + | chr1.1:56374640-56374659 | None:intergenic | 55.0% | |
| CTTCGACAGGTACTCCTGCT+AGG | - | chr1.1:56373064-56373083 | MS.gene52997:intron | 55.0% | |
| GACCCTTGTAAGGCCATCGA+AGG | + | chr1.1:56374643-56374662 | None:intergenic | 55.0% | |
| ! | CCACCTTCGATGGCCTTACA+AGG | - | chr1.1:56374637-56374656 | MS.gene52997:intron | 55.0% |
| ! | GTCACGAGGTGTCAGCTCAA+TGG | - | chr1.1:56373250-56373269 | MS.gene52997:CDS | 55.0% |
| GGCCATCGAAGGTGGCGATC+AGG | + | chr1.1:56374632-56374651 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 56372718 | 56375205 | 56372718 | ID=MS.gene52997 |
| chr1.1 | mRNA | 56372718 | 56375205 | 56372718 | ID=MS.gene52997.t1;Parent=MS.gene52997 |
| chr1.1 | exon | 56374773 | 56375205 | 56374773 | ID=MS.gene52997.t1.exon1;Parent=MS.gene52997.t1 |
| chr1.1 | CDS | 56374773 | 56375205 | 56374773 | ID=cds.MS.gene52997.t1;Parent=MS.gene52997.t1 |
| chr1.1 | exon | 56374310 | 56374492 | 56374310 | ID=MS.gene52997.t1.exon2;Parent=MS.gene52997.t1 |
| chr1.1 | CDS | 56374310 | 56374492 | 56374310 | ID=cds.MS.gene52997.t1;Parent=MS.gene52997.t1 |
| chr1.1 | exon | 56373112 | 56373929 | 56373112 | ID=MS.gene52997.t1.exon3;Parent=MS.gene52997.t1 |
| chr1.1 | CDS | 56373112 | 56373929 | 56373112 | ID=cds.MS.gene52997.t1;Parent=MS.gene52997.t1 |
| chr1.1 | exon | 56372718 | 56372969 | 56372718 | ID=MS.gene52997.t1.exon4;Parent=MS.gene52997.t1 |
| chr1.1 | CDS | 56372718 | 56372969 | 56372718 | ID=cds.MS.gene52997.t1;Parent=MS.gene52997.t1 |
| Gene Sequence |
| Protein sequence |
