Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71450.t1 | XP_003590788.2 | 95.7 | 561 | 24 | 0 | 1 | 561 | 1 | 561 | 7.10E-302 | 1045.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71450.t1 | G7I3Y5 | 95.7 | 561 | 24 | 0 | 1 | 561 | 1 | 561 | 5.1e-302 | 1045.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene71450 | 0.828197 | 1.03E-54 | -1.69E-46 |
| MS.gene049151 | MS.gene71450 | 0.804991 | 1.66E-49 | -1.69E-46 |
| MS.gene049153 | MS.gene71450 | 0.829887 | 4.00E-55 | -1.69E-46 |
| MS.gene049356 | MS.gene71450 | 0.80591 | 1.06E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene71450 | 0.842483 | 2.52E-58 | -1.69E-46 |
| MS.gene049758 | MS.gene71450 | 0.813286 | 2.76E-51 | -1.69E-46 |
| MS.gene049910 | MS.gene71450 | 0.833312 | 5.73E-56 | -1.69E-46 |
| MS.gene050128 | MS.gene71450 | 0.813448 | 2.55E-51 | -1.69E-46 |
| MS.gene050168 | MS.gene71450 | 0.802062 | 6.70E-49 | -1.69E-46 |
| MS.gene050226 | MS.gene71450 | 0.819235 | 1.29E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene71450 | 0.80995 | 1.47E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene71450 | 0.800323 | 1.52E-48 | -1.69E-46 |
| MS.gene050301 | MS.gene71450 | 0.812414 | 4.29E-51 | -1.69E-46 |
| MS.gene050398 | MS.gene71450 | 0.803903 | 2.79E-49 | -1.69E-46 |
| MS.gene050426 | MS.gene71450 | -0.819093 | 1.39E-52 | -1.69E-46 |
| MS.gene050642 | MS.gene71450 | 0.82095 | 5.23E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene71450 | 0.833528 | 5.06E-56 | -1.69E-46 |
| MS.gene050971 | MS.gene71450 | 0.838929 | 2.15E-57 | -1.69E-46 |
| MS.gene051525 | MS.gene71450 | 0.807844 | 4.14E-50 | -1.69E-46 |
| MS.gene051535 | MS.gene71450 | 0.808814 | 2.57E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene71450 | 0.846443 | 2.17E-59 | -1.69E-46 |
| MS.gene051983 | MS.gene71450 | 0.832865 | 7.41E-56 | -1.69E-46 |
| MS.gene052011 | MS.gene71450 | -0.839525 | 1.50E-57 | -1.69E-46 |
| MS.gene052077 | MS.gene71450 | 0.894056 | 3.53E-75 | -1.69E-46 |
| MS.gene052135 | MS.gene71450 | 0.804702 | 1.90E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene71450 | 0.86112 | 1.28E-63 | -1.69E-46 |
| MS.gene052253 | MS.gene71450 | 0.872556 | 2.91E-67 | -1.69E-46 |
| MS.gene052255 | MS.gene71450 | 0.813326 | 2.71E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene71450 | 0.847953 | 8.34E-60 | -1.69E-46 |
| MS.gene052389 | MS.gene71450 | 0.828631 | 8.07E-55 | -1.69E-46 |
| MS.gene053073 | MS.gene71450 | 0.809493 | 1.84E-50 | -1.69E-46 |
| MS.gene05316 | MS.gene71450 | 0.868968 | 4.40E-66 | -1.69E-46 |
| MS.gene053494 | MS.gene71450 | 0.847813 | 9.12E-60 | -1.69E-46 |
| MS.gene053557 | MS.gene71450 | 0.802232 | 6.19E-49 | -1.69E-46 |
| MS.gene053577 | MS.gene71450 | 0.8146 | 1.42E-51 | -1.69E-46 |
| MS.gene053758 | MS.gene71450 | 0.817092 | 3.94E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene71450 | 0.834774 | 2.47E-56 | -1.69E-46 |
| MS.gene053928 | MS.gene71450 | 0.805505 | 1.29E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene71450 | 0.823446 | 1.38E-53 | -1.69E-46 |
| MS.gene054012 | MS.gene71450 | 0.80915 | 2.18E-50 | -1.69E-46 |
| MS.gene054013 | MS.gene71450 | 0.804346 | 2.26E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene71450 | 0.86942 | 3.14E-66 | -1.69E-46 |
| MS.gene054349 | MS.gene71450 | 0.88522 | 9.81E-72 | -1.69E-46 |
| MS.gene054379 | MS.gene71450 | 0.814017 | 1.91E-51 | -1.69E-46 |
| MS.gene054542 | MS.gene71450 | 0.810241 | 1.27E-50 | -1.69E-46 |
| MS.gene054556 | MS.gene71450 | 0.808742 | 2.67E-50 | -1.69E-46 |
| MS.gene054826 | MS.gene71450 | 0.812649 | 3.81E-51 | -1.69E-46 |
| MS.gene054918 | MS.gene71450 | 0.815993 | 6.95E-52 | -1.69E-46 |
| MS.gene054930 | MS.gene71450 | 0.848058 | 7.80E-60 | -1.69E-46 |
| MS.gene054983 | MS.gene71450 | 0.800801 | 1.22E-48 | -1.69E-46 |
| MS.gene055116 | MS.gene71450 | 0.843241 | 1.58E-58 | -1.69E-46 |
| MS.gene05553 | MS.gene71450 | 0.843278 | 1.55E-58 | -1.69E-46 |
| MS.gene055604 | MS.gene71450 | 0.81787 | 2.64E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene71450 | 0.850627 | 1.50E-60 | -1.69E-46 |
| MS.gene056511 | MS.gene71450 | 0.801337 | 9.44E-49 | -1.69E-46 |
| MS.gene056659 | MS.gene71450 | 0.836938 | 6.98E-57 | -1.69E-46 |
| MS.gene056723 | MS.gene71450 | 0.825873 | 3.70E-54 | -1.69E-46 |
| MS.gene056979 | MS.gene71450 | 0.825518 | 4.49E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene71450 | 0.83036 | 3.07E-55 | -1.69E-46 |
| MS.gene057396 | MS.gene71450 | 0.874577 | 6.06E-68 | -1.69E-46 |
| MS.gene057448 | MS.gene71450 | 0.810645 | 1.04E-50 | -1.69E-46 |
| MS.gene057449 | MS.gene71450 | 0.801353 | 9.37E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene71450 | 0.800824 | 1.20E-48 | -1.69E-46 |
| MS.gene057610 | MS.gene71450 | 0.827276 | 1.71E-54 | -1.69E-46 |
| MS.gene057749 | MS.gene71450 | 0.821542 | 3.82E-53 | -1.69E-46 |
| MS.gene057750 | MS.gene71450 | 0.802308 | 5.97E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene71450 | 0.815155 | 1.07E-51 | -1.69E-46 |
| MS.gene057825 | MS.gene71450 | 0.821146 | 4.72E-53 | -1.69E-46 |
| MS.gene057929 | MS.gene71450 | 0.831474 | 1.64E-55 | -1.69E-46 |
| MS.gene058002 | MS.gene71450 | 0.812971 | 3.24E-51 | -1.69E-46 |
| MS.gene058007 | MS.gene71450 | 0.83394 | 3.99E-56 | -1.69E-46 |
| MS.gene058072 | MS.gene71450 | 0.813184 | 2.91E-51 | -1.69E-46 |
| MS.gene058075 | MS.gene71450 | 0.808703 | 2.72E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene71450 | 0.804925 | 1.71E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene71450 | 0.828445 | 8.95E-55 | -1.69E-46 |
| MS.gene058435 | MS.gene71450 | 0.839286 | 1.74E-57 | -1.69E-46 |
| MS.gene058710 | MS.gene71450 | 0.835701 | 1.44E-56 | -1.69E-46 |
| MS.gene05872 | MS.gene71450 | 0.805995 | 1.02E-49 | -1.69E-46 |
| MS.gene05886 | MS.gene71450 | 0.806462 | 8.13E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene71450 | 0.827779 | 1.30E-54 | -1.69E-46 |
| MS.gene058949 | MS.gene71450 | 0.836199 | 1.08E-56 | -1.69E-46 |
| MS.gene059011 | MS.gene71450 | 0.834605 | 2.72E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene71450 | 0.843716 | 1.18E-58 | -1.69E-46 |
| MS.gene059082 | MS.gene71450 | 0.807032 | 6.16E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene71450 | 0.877918 | 4.27E-69 | -1.69E-46 |
| MS.gene059294 | MS.gene71450 | 0.807333 | 5.32E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene71450 | 0.852147 | 5.59E-61 | -1.69E-46 |
| MS.gene059462 | MS.gene71450 | 0.837619 | 4.67E-57 | -1.69E-46 |
| MS.gene059597 | MS.gene71450 | 0.816527 | 5.28E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene71450 | 0.823554 | 1.30E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene71450 | 0.812635 | 3.84E-51 | -1.69E-46 |
| MS.gene059695 | MS.gene71450 | 0.807871 | 4.09E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene71450 | 0.871488 | 6.58E-67 | -1.69E-46 |
| MS.gene05984 | MS.gene71450 | 0.828857 | 7.12E-55 | -1.69E-46 |
| MS.gene059876 | MS.gene71450 | 0.868502 | 6.23E-66 | -1.69E-46 |
| MS.gene060247 | MS.gene71450 | 0.857497 | 1.57E-62 | -1.69E-46 |
| MS.gene060495 | MS.gene71450 | 0.873628 | 1.27E-67 | -1.69E-46 |
| MS.gene060683 | MS.gene71450 | 0.809622 | 1.73E-50 | -1.69E-46 |
| MS.gene061002 | MS.gene71450 | 0.803511 | 3.37E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene71450 | 0.862302 | 5.58E-64 | -1.69E-46 |
| MS.gene061159 | MS.gene71450 | 0.825769 | 3.91E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71450.t1 | MTR_1g075280 | 95.722 | 561 | 24 | 0 | 1 | 561 | 1 | 561 | 0.0 | 1116 |
| MS.gene71450.t1 | MTR_1g075280 | 88.770 | 561 | 24 | 1 | 1 | 561 | 1 | 522 | 0.0 | 1018 |
| MS.gene71450.t1 | MTR_5g007040 | 34.983 | 303 | 134 | 8 | 207 | 462 | 94 | 380 | 3.81e-32 | 132 |
| MS.gene71450.t1 | MTR_5g007040 | 49.038 | 104 | 47 | 1 | 1 | 104 | 9 | 106 | 2.16e-22 | 102 |
| MS.gene71450.t1 | MTR_6g046940 | 28.261 | 414 | 204 | 16 | 207 | 556 | 94 | 478 | 2.61e-21 | 98.6 |
| MS.gene71450.t1 | MTR_6g046940 | 46.154 | 104 | 50 | 1 | 1 | 104 | 9 | 106 | 1.22e-19 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 92 sgRNAs with CRISPR-Local
Find 151 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAATCTTCTACTATTTC+AGG | 0.180626 | 1.3:-53878851 | MS.gene71450:intron |
| ACAATATGTGGACTGAAAAT+TGG | 0.244513 | 1.3:+53877750 | None:intergenic |
| GAATCTCTCAATCTCGAAAA+AGG | 0.266480 | 1.3:+53877327 | None:intergenic |
| GCCAGGATCTGGTGATAATT+TGG | 0.270512 | 1.3:-53879017 | MS.gene71450:intron |
| TTAAGACTGAAACCTTTATC+TGG | 0.299755 | 1.3:+53879569 | None:intergenic |
| ATGACTTCAAAACCAGTCTT+TGG | 0.306670 | 1.3:+53879719 | None:intergenic |
| CCTGTATCATGTTCTTGATA+TGG | 0.316401 | 1.3:+53879314 | None:intergenic |
| ATCCTCTATCTGAAATAGTT+TGG | 0.324431 | 1.3:-53879595 | MS.gene71450:CDS |
| TCTTGATATGGTGTATATTC+TGG | 0.346161 | 1.3:+53879326 | None:intergenic |
| TGTTTGTTGGCATTGTTTGA+TGG | 0.368003 | 1.3:+53877266 | None:intergenic |
| TTGATGAGACATTCTTGTTC+CGG | 0.369355 | 1.3:+53877711 | None:intergenic |
| AGCTAAAATATCTGAGATAA+TGG | 0.370067 | 1.3:-53878882 | MS.gene71450:CDS |
| CCTTCTTTGTAATGTTTCCT+TGG | 0.372511 | 1.3:+53878369 | None:intergenic |
| ATAGTTTGGACTCCAGATAA+AGG | 0.389342 | 1.3:-53879581 | MS.gene71450:CDS |
| TGCTGGAGGAACATCTGATT+TGG | 0.391359 | 1.3:-53878981 | MS.gene71450:CDS |
| TTTGAAAACTATCATAATCC+TGG | 0.393605 | 1.3:-53878045 | MS.gene71450:CDS |
| GTAAATTACCAACTGGACTT+TGG | 0.394116 | 1.3:+53878439 | None:intergenic |
| TCAAGATGAGGAGAAGTCTT+TGG | 0.394857 | 1.3:-53877854 | MS.gene71450:CDS |
| TTGGAGAAAATTAACTCTGC+TGG | 0.405206 | 1.3:-53878998 | MS.gene71450:CDS |
| TGACTCTCATTCTTATTGTA+TGG | 0.410611 | 1.3:+53878075 | None:intergenic |
| TATTTCAGATAGAGGATCAT+TGG | 0.411235 | 1.3:+53879601 | None:intergenic |
| GAACACATGATTGAAGATAA+AGG | 0.411777 | 1.3:-53878285 | MS.gene71450:CDS |
| TGGTTTCAAACATGACTAAA+GGG | 0.429791 | 1.3:-53877891 | MS.gene71450:CDS |
| TGGTAGACTATTCTGTTTGT+TGG | 0.430093 | 1.3:+53877253 | None:intergenic |
| TCCATGTTTGCAAAGAGGTT+AGG | 0.431116 | 1.3:-53877291 | MS.gene71450:CDS |
| ATCATCCGGCATTAATCATT+CGG | 0.432649 | 1.3:-53878235 | MS.gene71450:CDS |
| TTGGTTTCAAACATGACTAA+AGG | 0.435096 | 1.3:-53877892 | MS.gene71450:CDS |
| TCATAGCTCATTCATGAACT+TGG | 0.436526 | 1.3:-53877911 | MS.gene71450:CDS |
| GGAGAATTTAATGAACCATT+GGG | 0.439177 | 1.3:-53878945 | MS.gene71450:CDS |
| GGCCATCGAAGGTGGCGATC+AGG | 0.439496 | 1.3:+53877814 | None:intergenic |
| TGAACTTGTAAATTACCAAC+TGG | 0.441384 | 1.3:+53878432 | None:intergenic |
| CGATGTCGAACCTAGTAGTA+TGG | 0.441570 | 1.3:-53879501 | MS.gene71450:CDS |
| AGCTGCTTCAATTTCAATGC+AGG | 0.443120 | 1.3:+53878901 | None:intergenic |
| GTGTTCTTCTTTACATCCTC+AGG | 0.443827 | 1.3:-53877653 | MS.gene71450:intron |
| GTCCAAACTATTTCAGATAG+AGG | 0.446372 | 1.3:+53879593 | None:intergenic |
| AGGAGAATTTAATGAACCAT+TGG | 0.446595 | 1.3:-53878946 | MS.gene71450:CDS |
| CTTCTCATCTTTAAGACCAA+TGG | 0.453090 | 1.3:+53877395 | None:intergenic |
| TTGTAATGTTTCCTTGGTGA+AGG | 0.453555 | 1.3:+53878375 | None:intergenic |
| AAAGAGGCTTCTTCTTCCAT+TGG | 0.453603 | 1.3:-53877411 | MS.gene71450:CDS |
| CCACCTTCGATGGCCTTACA+AGG | 0.455789 | 1.3:-53877806 | MS.gene71450:CDS |
| GGAACATATTTGATATCATC+CGG | 0.456936 | 1.3:-53878249 | MS.gene71450:CDS |
| AATTTACAAGTTCATCAAGA+TGG | 0.458602 | 1.3:-53878423 | MS.gene71450:CDS |
| CCAAGGAAACATTACAAAGA+AGG | 0.463419 | 1.3:-53878369 | MS.gene71450:CDS |
| GATAAAGGTTCTAATCCTCT+TGG | 0.465705 | 1.3:-53878270 | MS.gene71450:CDS |
| GGAACATCTGATTTGGCAAA+TGG | 0.468310 | 1.3:-53878974 | MS.gene71450:CDS |
| GGACTGAAAATTGGATTTGA+AGG | 0.473417 | 1.3:+53877759 | None:intergenic |
| TGCACTAAAACGTCTTCTGC+AGG | 0.475147 | 1.3:+53879437 | None:intergenic |
| ACAATATGCTGATAACCAAT+TGG | 0.476134 | 1.3:-53877472 | MS.gene71450:CDS |
| TCCGGAGCTTGACCCTTGTA+AGG | 0.477553 | 1.3:+53877793 | None:intergenic |
| GCCTTACAAGGGTCAAGCTC+CGG | 0.484930 | 1.3:-53877794 | MS.gene71450:CDS |
| TGATTTGGCAAATGGTCAAA+AGG | 0.486780 | 1.3:-53878966 | MS.gene71450:CDS |
| AAGAGTGACGTTTCTTCGAC+AGG | 0.500026 | 1.3:-53879392 | MS.gene71450:CDS |
| GAAACATTACAAAGAAGGCT+TGG | 0.503191 | 1.3:-53878364 | MS.gene71450:CDS |
| CTCAAAACACTGACAATATG+TGG | 0.503790 | 1.3:+53877738 | None:intergenic |
| CTTCGACAGGTACTCCTGCT+AGG | 0.511003 | 1.3:-53879379 | MS.gene71450:CDS |
| CACAAATATTCTTCTCCCAA+TGG | 0.514033 | 1.3:+53878930 | None:intergenic |
| AATATCTGAGATAATGGAAG+AGG | 0.514653 | 1.3:-53878876 | MS.gene71450:CDS |
| ATTGAGAGATTCAGAGGCAA+TGG | 0.519119 | 1.3:-53877316 | MS.gene71450:CDS |
| CATTGGTCTTAAAGATGAGA+AGG | 0.529065 | 1.3:-53877394 | MS.gene71450:CDS |
| TGGTGTATATTCTGGAAAGA+TGG | 0.529676 | 1.3:+53879334 | None:intergenic |
| ATTTACAAGTTCATCAAGAT+GGG | 0.530667 | 1.3:-53878422 | MS.gene71450:CDS |
| TTATTCTAGTCAATGTCATA+AGG | 0.530823 | 1.3:-53879690 | MS.gene71450:CDS |
| AAGAAAATGAAACCAAAGAC+TGG | 0.532411 | 1.3:-53879731 | None:intergenic |
| TCGCTTGTTGAATTCCTAGC+AGG | 0.535881 | 1.3:+53879365 | None:intergenic |
| TAGCATCATCTCATTGACCT+TGG | 0.536137 | 1.3:+53878098 | None:intergenic |
| CCATATCAAGAACATGATAC+AGG | 0.536521 | 1.3:-53879314 | MS.gene71450:intron |
| ATCCTGATCGCCACCTTCGA+TGG | 0.538722 | 1.3:-53877816 | MS.gene71450:CDS |
| ACCTAACCTCTTTGCAAACA+TGG | 0.543122 | 1.3:+53877290 | None:intergenic |
| TTCGAGATTGAGAGATTCAG+AGG | 0.550691 | 1.3:-53877322 | MS.gene71450:CDS |
| AGAGCAAGAACCATACTACT+AGG | 0.554225 | 1.3:+53879491 | None:intergenic |
| AGATGGGTCTAAACTTAGCA+TGG | 0.563633 | 1.3:-53878406 | MS.gene71450:CDS |
| TTGCAAACTGAGTCAAGTGC+AGG | 0.567039 | 1.3:-53879662 | MS.gene71450:CDS |
| TGGTTTCCATGTTTGCAAAG+AGG | 0.574375 | 1.3:-53877296 | MS.gene71450:CDS |
| TTCGACATCGCGCAAAAGCG+AGG | 0.575471 | 1.3:+53879514 | None:intergenic |
| ACAAGAATGTCTCATCAAGT+AGG | 0.578856 | 1.3:-53877706 | MS.gene71450:CDS |
| TGAGCTATGATGATTAACAT+AGG | 0.579724 | 1.3:+53877924 | None:intergenic |
| GACCCTTGTAAGGCCATCGA+AGG | 0.580322 | 1.3:+53877803 | None:intergenic |
| TTTGCGCATATAACAGCAAG+AGG | 0.582464 | 1.3:+53879413 | None:intergenic |
| ATTGGTCTTAAAGATGAGAA+GGG | 0.585207 | 1.3:-53877393 | MS.gene71450:CDS |
| CCTTGTAAGGCCATCGAAGG+TGG | 0.590280 | 1.3:+53877806 | None:intergenic |
| GAGAAAATTAACTCTGCTGG+AGG | 0.604426 | 1.3:-53878995 | MS.gene71450:CDS |
| CAGATCAACCAAAGTCCAGT+TGG | 0.604525 | 1.3:-53878447 | MS.gene71450:CDS |
| GTCTTCTGCAGGTTTATCTG+TGG | 0.625174 | 1.3:+53879448 | None:intergenic |
| GGATACTAGTGTTGACAACA+TGG | 0.626835 | 1.3:-53878343 | MS.gene71450:CDS |
| AAGAAGAACACCGTCTGCAG+AGG | 0.634486 | 1.3:+53877665 | None:intergenic |
| CGAGCAATAGCCTCTGCAGA+CGG | 0.638779 | 1.3:-53877675 | MS.gene71450:CDS |
| AGATGAGAAGGGAAACAATG+AGG | 0.641689 | 1.3:-53877382 | MS.gene71450:CDS |
| CTCACAATCTAGTCAAGATG+AGG | 0.644826 | 1.3:-53877866 | MS.gene71450:CDS |
| CACCTTCGATGGCCTTACAA+GGG | 0.654668 | 1.3:-53877805 | MS.gene71450:CDS |
| GATATCAAATATGTTCCAAG+AGG | 0.664662 | 1.3:+53878255 | None:intergenic |
| TGGAACAAAATCCTTCACCA+AGG | 0.714111 | 1.3:-53878386 | MS.gene71450:CDS |
| TACTAGTGTTGACAACATGG+TGG | 0.817206 | 1.3:-53878340 | MS.gene71450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCATGATAGTATCTTAAAA+TGG | + | chr1.3:53878197-53878216 | None:intergenic | 20.0% |
| !! | TAAAAATTAATAACTTCAGC+AGG | + | chr1.3:53878450-53878469 | None:intergenic | 20.0% |
| !! | TGATAATAAAAACAAATCCA+AGG | - | chr1.3:53878870-53878889 | MS.gene71450:CDS | 20.0% |
| !! | TTTACAAAATAGAATCAGTA+AGG | - | chr1.3:53879420-53879439 | MS.gene71450:CDS | 20.0% |
| !!! | AAGGTTTTAAAGGAAAAAAT+TGG | + | chr1.3:53877923-53877942 | None:intergenic | 20.0% |
| !!! | ATATAAATTTTCCTCAATCA+TGG | - | chr1.3:53878259-53878278 | MS.gene71450:CDS | 20.0% |
| !!! | GGATTTGTTTTTATTATCAT+TGG | + | chr1.3:53878869-53878888 | None:intergenic | 20.0% |
| !!! | TATGATGAAGTTTTTTGAAT+TGG | + | chr1.3:53879033-53879052 | None:intergenic | 20.0% |
| !!! | TTTAAGATACTATCATGATT+TGG | - | chr1.3:53878198-53878217 | MS.gene71450:CDS | 20.0% |
| ! | AACAAATCTTCTACTATTTC+AGG | - | chr1.3:53878134-53878153 | MS.gene71450:CDS | 25.0% |
| ! | AATTTACAAGTTCATCAAGA+TGG | - | chr1.3:53878562-53878581 | MS.gene71450:intron | 25.0% |
| ! | ATTAATTAAAGTGACACTAG+TGG | + | chr1.3:53877883-53877902 | None:intergenic | 25.0% |
| ! | ATTTACAAGTTCATCAAGAT+GGG | - | chr1.3:53878563-53878582 | MS.gene71450:intron | 25.0% |
| ! | CAGTAATTCAAATGAGAAAT+TGG | + | chr1.3:53879472-53879491 | None:intergenic | 25.0% |
| ! | CATTAATCATTCGGAAAAAA+TGG | - | chr1.3:53878759-53878778 | MS.gene71450:intron | 25.0% |
| ! | TGGCTATTGAGAAAATTTAT+AGG | + | chr1.3:53879363-53879382 | None:intergenic | 25.0% |
| ! | TTATTCTAGTCAATGTCATA+AGG | - | chr1.3:53877295-53877314 | MS.gene71450:CDS | 25.0% |
| ! | TTTAGATATGCAAAAGAGAA+TGG | + | chr1.3:53878488-53878507 | None:intergenic | 25.0% |
| ! | TTTGAAAACTATCATAATCC+TGG | - | chr1.3:53878940-53878959 | MS.gene71450:CDS | 25.0% |
| !! | AGCTAAAATATCTGAGATAA+TGG | - | chr1.3:53878103-53878122 | MS.gene71450:CDS | 25.0% |
| !! | TTGAAGGAATTTTTAATCTC+CGG | + | chr1.3:53879213-53879232 | None:intergenic | 25.0% |
| !!! | GAATTGTTTTATCAACAAAG+AGG | - | chr1.3:53879558-53879577 | MS.gene71450:CDS | 25.0% |
| !!! | TTGAGCAAAAAAGGTTTTAA+AGG | + | chr1.3:53877933-53877952 | None:intergenic | 25.0% |
| ACAATATGCTGATAACCAAT+TGG | - | chr1.3:53879513-53879532 | MS.gene71450:CDS | 30.0% | |
| ACATCATGATTGAGCAAAAA+AGG | + | chr1.3:53877942-53877961 | None:intergenic | 30.0% | |
| AGGAGAATTTAATGAACCAT+TGG | - | chr1.3:53878039-53878058 | MS.gene71450:CDS | 30.0% | |
| CAATTAACACTGCATATCAA+AGG | - | chr1.3:53878292-53878311 | MS.gene71450:CDS | 30.0% | |
| CTTTGATATGCAGTGTTAAT+TGG | + | chr1.3:53878294-53878313 | None:intergenic | 30.0% | |
| GAACACATGATTGAAGATAA+AGG | - | chr1.3:53878700-53878719 | MS.gene71450:intron | 30.0% | |
| GATATCAAATATGTTCCAAG+AGG | + | chr1.3:53878733-53878752 | None:intergenic | 30.0% | |
| GGAACATATTTGATATCATC+CGG | - | chr1.3:53878736-53878755 | MS.gene71450:intron | 30.0% | |
| GGAGAATTTAATGAACCATT+GGG | - | chr1.3:53878040-53878059 | MS.gene71450:CDS | 30.0% | |
| GGTAAATATAACATCCCTTA+TGG | - | chr1.3:53877692-53877711 | MS.gene71450:CDS | 30.0% | |
| TATTTCAGATAGAGGATCAT+TGG | + | chr1.3:53877387-53877406 | None:intergenic | 30.0% | |
| TCTTGATATGGTGTATATTC+TGG | + | chr1.3:53877662-53877681 | None:intergenic | 30.0% | |
| TGAACTTGTAAATTACCAAC+TGG | + | chr1.3:53878556-53878575 | None:intergenic | 30.0% | |
| TGACTCTCATTCTTATTGTA+TGG | + | chr1.3:53878913-53878932 | None:intergenic | 30.0% | |
| TGAGCTATGATGATTAACAT+AGG | + | chr1.3:53879064-53879083 | None:intergenic | 30.0% | |
| TGGTTTCAAACATGACTAAA+GGG | - | chr1.3:53879094-53879113 | MS.gene71450:intron | 30.0% | |
| TTAAGACTGAAACCTTTATC+TGG | + | chr1.3:53877419-53877438 | None:intergenic | 30.0% | |
| TTCTCATTTGAATTACTGCA+AGG | - | chr1.3:53879474-53879493 | MS.gene71450:CDS | 30.0% | |
| TTGGTTTCAAACATGACTAA+AGG | - | chr1.3:53879093-53879112 | MS.gene71450:intron | 30.0% | |
| ! | ACAATATGTGGACTGAAAAT+TGG | + | chr1.3:53879238-53879257 | None:intergenic | 30.0% |
| ! | ATCCTCTATCTGAAATAGTT+TGG | - | chr1.3:53877390-53877409 | MS.gene71450:CDS | 30.0% |
| ! | TCATAATCCTGGATTGTTTT+TGG | - | chr1.3:53878951-53878970 | MS.gene71450:CDS | 30.0% |
| ! | TGCTGTCATAATTGATTCTT+TGG | - | chr1.3:53878161-53878180 | MS.gene71450:CDS | 30.0% |
| ! | TTTTAGTCTCAATGACCAAT+TGG | + | chr1.3:53879531-53879550 | None:intergenic | 30.0% |
| ! | TTTTTCCGAATGATTAATGC+CGG | + | chr1.3:53878758-53878777 | None:intergenic | 30.0% |
| !! | AATATCTGAGATAATGGAAG+AGG | - | chr1.3:53878109-53878128 | MS.gene71450:CDS | 30.0% |
| !! | ATTGGTCTTAAAGATGAGAA+GGG | - | chr1.3:53879592-53879611 | MS.gene71450:CDS | 30.0% |
| !! | GGATTTATTTTCCATGATTG+AGG | + | chr1.3:53878273-53878292 | None:intergenic | 30.0% |
| !! | GTGATATTTTTCAGTGTTCA+TGG | + | chr1.3:53879383-53879402 | None:intergenic | 30.0% |
| AAATTTATAGGCCTAACCTG+AGG | + | chr1.3:53879351-53879370 | None:intergenic | 35.0% | |
| ACAAGAATGTCTCATCAAGT+AGG | - | chr1.3:53879279-53879298 | MS.gene71450:intron | 35.0% | |
| ATAGTTTGGACTCCAGATAA+AGG | - | chr1.3:53877404-53877423 | MS.gene71450:CDS | 35.0% | |
| ATCATCCGGCATTAATCATT+CGG | - | chr1.3:53878750-53878769 | MS.gene71450:intron | 35.0% | |
| ATGACTTCAAAACCAGTCTT+TGG | + | chr1.3:53877269-53877288 | None:intergenic | 35.0% | |
| CACAAATATTCTTCTCCCAA+TGG | + | chr1.3:53878058-53878077 | None:intergenic | 35.0% | |
| CCAAGGAAACATTACAAAGA+AGG | - | chr1.3:53878616-53878635 | MS.gene71450:intron | 35.0% | |
| CCATATCAAGAACATGATAC+AGG | - | chr1.3:53877671-53877690 | MS.gene71450:CDS | 35.0% | |
| CCTGTATCATGTTCTTGATA+TGG | + | chr1.3:53877674-53877693 | None:intergenic | 35.0% | |
| CCTTCTTTGTAATGTTTCCT+TGG | + | chr1.3:53878619-53878638 | None:intergenic | 35.0% | |
| CTCAAAACACTGACAATATG+TGG | + | chr1.3:53879250-53879269 | None:intergenic | 35.0% | |
| GAAACATTACAAAGAAGGCT+TGG | - | chr1.3:53878621-53878640 | MS.gene71450:intron | 35.0% | |
| GAATCTCTCAATCTCGAAAA+AGG | + | chr1.3:53879661-53879680 | None:intergenic | 35.0% | |
| GATTTGAATTTCATCCCTTC+AGG | + | chr1.3:53877837-53877856 | None:intergenic | 35.0% | |
| GGACTGAAAATTGGATTTGA+AGG | + | chr1.3:53879229-53879248 | None:intergenic | 35.0% | |
| GTAAATTACCAACTGGACTT+TGG | + | chr1.3:53878549-53878568 | None:intergenic | 35.0% | |
| GTCCAAACTATTTCAGATAG+AGG | + | chr1.3:53877395-53877414 | None:intergenic | 35.0% | |
| TACATCTGTAGGATCCATAA+GGG | + | chr1.3:53877709-53877728 | None:intergenic | 35.0% | |
| TCATAGCTCATTCATGAACT+TGG | - | chr1.3:53879074-53879093 | MS.gene71450:intron | 35.0% | |
| TGAGTTTGCAGTAAGAACTT+TGG | - | chr1.3:53877737-53877756 | MS.gene71450:CDS | 35.0% | |
| TTATGGATCCTACAGATGTA+GGG | - | chr1.3:53877709-53877728 | MS.gene71450:CDS | 35.0% | |
| TTGATGAGACATTCTTGTTC+CGG | + | chr1.3:53879277-53879296 | None:intergenic | 35.0% | |
| TTGGAGAAAATTAACTCTGC+TGG | - | chr1.3:53877987-53878006 | MS.gene71450:CDS | 35.0% | |
| TTGTAATGTTTCCTTGGTGA+AGG | + | chr1.3:53878613-53878632 | None:intergenic | 35.0% | |
| ! | GAAGTATTTACTTTGTCACG+AGG | - | chr1.3:53877778-53877797 | MS.gene71450:CDS | 35.0% |
| ! | GATAAAGGTTCTAATCCTCT+TGG | - | chr1.3:53878715-53878734 | MS.gene71450:intron | 35.0% |
| ! | TGATTTGGCAAATGGTCAAA+AGG | - | chr1.3:53878019-53878038 | MS.gene71450:CDS | 35.0% |
| ! | TGGTGTATATTCTGGAAAGA+TGG | + | chr1.3:53877654-53877673 | None:intergenic | 35.0% |
| ! | TGTTTGTTGGCATTGTTTGA+TGG | + | chr1.3:53879722-53879741 | None:intergenic | 35.0% |
| !! | CATTGGTCTTAAAGATGAGA+AGG | - | chr1.3:53879591-53879610 | MS.gene71450:CDS | 35.0% |
| !! | CTTCTCATCTTTAAGACCAA+TGG | + | chr1.3:53879593-53879612 | None:intergenic | 35.0% |
| AAAGAGGCTTCTTCTTCCAT+TGG | - | chr1.3:53879574-53879593 | MS.gene71450:CDS | 40.0% | |
| AATCATGATGTTGATCTGCC+AGG | - | chr1.3:53877951-53877970 | MS.gene71450:CDS | 40.0% | |
| ACCTAACCTCTTTGCAAACA+TGG | + | chr1.3:53879698-53879717 | None:intergenic | 40.0% | |
| AGAATGCATTGTCGCATAAC+CGG | + | chr1.3:53878422-53878441 | None:intergenic | 40.0% | |
| AGAGCAAGAACCATACTACT+AGG | + | chr1.3:53877497-53877516 | None:intergenic | 40.0% | |
| AGATGAGAAGGGAAACAATG+AGG | - | chr1.3:53879603-53879622 | MS.gene71450:CDS | 40.0% | |
| AGATGGGTCTAAACTTAGCA+TGG | - | chr1.3:53878579-53878598 | MS.gene71450:intron | 40.0% | |
| AGCTGCTTCAATTTCAATGC+AGG | + | chr1.3:53878087-53878106 | None:intergenic | 40.0% | |
| ATTGAGAGATTCAGAGGCAA+TGG | - | chr1.3:53879669-53879688 | MS.gene71450:CDS | 40.0% | |
| CTACATCTGTAGGATCCATA+AGG | + | chr1.3:53877710-53877729 | None:intergenic | 40.0% | |
| CTACTTGCCAAAAACAATCC+AGG | + | chr1.3:53878961-53878980 | None:intergenic | 40.0% | |
| CTCACAATCTAGTCAAGATG+AGG | - | chr1.3:53879119-53879138 | MS.gene71450:intron | 40.0% | |
| CTTATGGATCCTACAGATGT+AGG | - | chr1.3:53877708-53877727 | MS.gene71450:CDS | 40.0% | |
| CTTCTTTACATCCTCAGGTT+AGG | - | chr1.3:53879337-53879356 | MS.gene71450:CDS | 40.0% | |
| GAGAAAATTAACTCTGCTGG+AGG | - | chr1.3:53877990-53878009 | MS.gene71450:CDS | 40.0% | |
| GATGAAATTCAAATCCCTCG+GGG | - | chr1.3:53877842-53877861 | MS.gene71450:CDS | 40.0% | |
| GGATGAAATTCAAATCCCTC+GGG | - | chr1.3:53877841-53877860 | MS.gene71450:CDS | 40.0% | |
| GGGATGAAATTCAAATCCCT+CGG | - | chr1.3:53877840-53877859 | MS.gene71450:CDS | 40.0% | |
| GTGTTCTTCTTTACATCCTC+AGG | - | chr1.3:53879332-53879351 | MS.gene71450:CDS | 40.0% | |
| TAGCATCATCTCATTGACCT+TGG | + | chr1.3:53878890-53878909 | None:intergenic | 40.0% | |
| TCAAGATGAGGAGAAGTCTT+TGG | - | chr1.3:53879131-53879150 | MS.gene71450:intron | 40.0% | |
| TCACATTTCCCTACATCTGT+AGG | + | chr1.3:53877720-53877739 | None:intergenic | 40.0% | |
| TCAGCTCAATGGTAAGAACT+TGG | - | chr1.3:53877803-53877822 | MS.gene71450:CDS | 40.0% | |
| TCCAAATTATCACCAGATCC+TGG | + | chr1.3:53877972-53877991 | None:intergenic | 40.0% | |
| TCCATGTTTGCAAAGAGGTT+AGG | - | chr1.3:53879694-53879713 | MS.gene71450:CDS | 40.0% | |
| TCTGCATCATGACTTTCTTC+AGG | - | chr1.3:53878514-53878533 | MS.gene71450:intron | 40.0% | |
| TGGAACAAAATCCTTCACCA+AGG | - | chr1.3:53878599-53878618 | MS.gene71450:intron | 40.0% | |
| TGGTTTCCATGTTTGCAAAG+AGG | - | chr1.3:53879689-53879708 | MS.gene71450:CDS | 40.0% | |
| TTCGAGATTGAGAGATTCAG+AGG | - | chr1.3:53879663-53879682 | MS.gene71450:CDS | 40.0% | |
| TTTGCGCATATAACAGCAAG+AGG | + | chr1.3:53877575-53877594 | None:intergenic | 40.0% | |
| ! | CTGCATATCAAAGGCATGTA+CGG | - | chr1.3:53878301-53878320 | MS.gene71450:CDS | 40.0% |
| ! | TTTCATCCCTTCAGGTTACA+CGG | + | chr1.3:53877829-53877848 | None:intergenic | 40.0% |
| !! | GGAACATCTGATTTGGCAAA+TGG | - | chr1.3:53878011-53878030 | MS.gene71450:CDS | 40.0% |
| !! | GGATACTAGTGTTGACAACA+TGG | - | chr1.3:53878642-53878661 | MS.gene71450:intron | 40.0% |
| !! | TACTAGTGTTGACAACATGG+TGG | - | chr1.3:53878645-53878664 | MS.gene71450:intron | 40.0% |
| !!! | CTGGATTGTTTTTGGCAAGT+AGG | - | chr1.3:53878959-53878978 | MS.gene71450:CDS | 40.0% |
| !!! | TGTCAGTGTTTTGAGAATGC+CGG | - | chr1.3:53879255-53879274 | MS.gene71450:intron | 40.0% |
| AAATTCAAATCCCTCGGGGA+CGG | - | chr1.3:53877846-53877865 | MS.gene71450:CDS | 45.0% | |
| AAGAGTGACGTTTCTTCGAC+AGG | - | chr1.3:53877593-53877612 | MS.gene71450:intron | 45.0% | |
| CAGATCAACCAAAGTCCAGT+TGG | - | chr1.3:53878538-53878557 | MS.gene71450:intron | 45.0% | |
| CGATGTCGAACCTAGTAGTA+TGG | - | chr1.3:53877484-53877503 | MS.gene71450:CDS | 45.0% | |
| GCCAGGATCTGGTGATAATT+TGG | - | chr1.3:53877968-53877987 | MS.gene71450:CDS | 45.0% | |
| GTAACCACATGTGTCAGTGT+CGG | + | chr1.3:53878343-53878362 | None:intergenic | 45.0% | |
| GTCTTCTGCAGGTTTATCTG+TGG | + | chr1.3:53877540-53877559 | None:intergenic | 45.0% | |
| TGCACTAAAACGTCTTCTGC+AGG | + | chr1.3:53877551-53877570 | None:intergenic | 45.0% | |
| TGCTGGAGGAACATCTGATT+TGG | - | chr1.3:53878004-53878023 | MS.gene71450:CDS | 45.0% | |
| TTGCAAACTGAGTCAAGTGC+AGG | - | chr1.3:53877323-53877342 | MS.gene71450:CDS | 45.0% | |
| ! | TCGCTTGTTGAATTCCTAGC+AGG | + | chr1.3:53877623-53877642 | None:intergenic | 45.0% |
| AACTTGGCCGTGTAACCTGA+AGG | - | chr1.3:53877819-53877838 | MS.gene71450:CDS | 50.0% | |
| AAGAAGAACACCGTCTGCAG+AGG | + | chr1.3:53879323-53879342 | None:intergenic | 50.0% | |
| ACTTGGCCGTGTAACCTGAA+GGG | - | chr1.3:53877820-53877839 | MS.gene71450:CDS | 50.0% | |
| CTACATGTCAGTGTCGTGTC+CGG | - | chr1.3:53878400-53878419 | MS.gene71450:CDS | 50.0% | |
| GATGTTGATCTGCCAGGATC+TGG | - | chr1.3:53877957-53877976 | MS.gene71450:CDS | 50.0% | |
| GTATCCGACACTGACACATG+TGG | - | chr1.3:53878336-53878355 | MS.gene71450:CDS | 50.0% | |
| ! | CACCTTCGATGGCCTTACAA+GGG | - | chr1.3:53879180-53879199 | MS.gene71450:intron | 50.0% |
| !! | CATTTTTCAACCGTCCCCGA+GGG | + | chr1.3:53877859-53877878 | None:intergenic | 50.0% |
| !! | TCATTTTTCAACCGTCCCCG+AGG | + | chr1.3:53877860-53877879 | None:intergenic | 50.0% |
| ATCCTGATCGCCACCTTCGA+TGG | - | chr1.3:53879169-53879188 | MS.gene71450:intron | 55.0% | |
| CCTTGTAAGGCCATCGAAGG+TGG | + | chr1.3:53879182-53879201 | None:intergenic | 55.0% | |
| CGAGCAATAGCCTCTGCAGA+CGG | - | chr1.3:53879310-53879329 | MS.gene71450:intron | 55.0% | |
| CTTCGACAGGTACTCCTGCT+AGG | - | chr1.3:53877606-53877625 | MS.gene71450:intron | 55.0% | |
| GACCCTTGTAAGGCCATCGA+AGG | + | chr1.3:53879185-53879204 | None:intergenic | 55.0% | |
| GCCTTACAAGGGTCAAGCTC+CGG | - | chr1.3:53879191-53879210 | MS.gene71450:intron | 55.0% | |
| TCCGGAGCTTGACCCTTGTA+AGG | + | chr1.3:53879195-53879214 | None:intergenic | 55.0% | |
| TTCGACATCGCGCAAAAGCG+AGG | + | chr1.3:53877474-53877493 | None:intergenic | 55.0% | |
| ! | CCACCTTCGATGGCCTTACA+AGG | - | chr1.3:53879179-53879198 | MS.gene71450:intron | 55.0% |
| ! | GTCACGAGGTGTCAGCTCAA+TGG | - | chr1.3:53877792-53877811 | MS.gene71450:CDS | 55.0% |
| GGCCATCGAAGGTGGCGATC+AGG | + | chr1.3:53879174-53879193 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 53877260 | 53879747 | 53877260 | ID=MS.gene71450 |
| chr1.3 | mRNA | 53877260 | 53879747 | 53877260 | ID=MS.gene71450.t1;Parent=MS.gene71450 |
| chr1.3 | exon | 53879315 | 53879747 | 53879315 | ID=MS.gene71450.t1.exon1;Parent=MS.gene71450.t1 |
| chr1.3 | CDS | 53879315 | 53879747 | 53879315 | ID=cds.MS.gene71450.t1;Parent=MS.gene71450.t1 |
| chr1.3 | exon | 53878852 | 53879034 | 53878852 | ID=MS.gene71450.t1.exon2;Parent=MS.gene71450.t1 |
| chr1.3 | CDS | 53878852 | 53879034 | 53878852 | ID=cds.MS.gene71450.t1;Parent=MS.gene71450.t1 |
| chr1.3 | exon | 53877654 | 53878471 | 53877654 | ID=MS.gene71450.t1.exon3;Parent=MS.gene71450.t1 |
| chr1.3 | CDS | 53877654 | 53878471 | 53877654 | ID=cds.MS.gene71450.t1;Parent=MS.gene71450.t1 |
| chr1.3 | exon | 53877260 | 53877511 | 53877260 | ID=MS.gene71450.t1.exon4;Parent=MS.gene71450.t1 |
| chr1.3 | CDS | 53877260 | 53877511 | 53877260 | ID=cds.MS.gene71450.t1;Parent=MS.gene71450.t1 |
| Gene Sequence |
| Protein sequence |
