AlfalfaGEDB | MS.gene76535

M. sativa cultivar XinJiangDaYe / MS.gene76535

KEGG: -
GO: -
PFAM: PF07911.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76535.t1	XP_003597686.1	99	196	2	0	1	196	1	196	3.30E-105	391

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76535.t1	G7II08	99.0	196	2	0	1	196	1	196	2.4e-105	391.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene76535	0.874258	7.77E-68	-1.69E-46
MS.gene049369	MS.gene76535	0.859112	5.18E-63	-1.69E-46
MS.gene049396	MS.gene76535	0.840383	8.99E-58	-1.69E-46
MS.gene050122	MS.gene76535	0.808475	3.04E-50	-1.69E-46
MS.gene050670	MS.gene76535	0.864777	9.52E-65	-1.69E-46
MS.gene050971	MS.gene76535	0.839379	1.64E-57	-1.69E-46
MS.gene05105	MS.gene76535	0.848015	8.02E-60	-1.69E-46
MS.gene051360	MS.gene76535	0.813928	2.00E-51	-1.69E-46
MS.gene051387	MS.gene76535	0.802317	5.94E-49	-1.69E-46
MS.gene051525	MS.gene76535	0.815616	8.44E-52	-1.69E-46
MS.gene051692	MS.gene76535	0.802716	4.92E-49	-1.69E-46
MS.gene052188	MS.gene76535	0.862149	6.22E-64	-1.69E-46
MS.gene052253	MS.gene76535	0.841557	4.42E-58	-1.69E-46
MS.gene052310	MS.gene76535	0.808042	3.76E-50	-1.69E-46
MS.gene052340	MS.gene76535	0.831985	1.22E-55	-1.69E-46
MS.gene052346	MS.gene76535	0.800414	1.46E-48	-1.69E-46
MS.gene052414	MS.gene76535	0.838154	3.40E-57	-1.69E-46
MS.gene052421	MS.gene76535	0.83378	4.38E-56	-1.69E-46
MS.gene052485	MS.gene76535	0.844286	8.30E-59	-1.69E-46
MS.gene052999	MS.gene76535	0.805218	1.48E-49	-1.69E-46
MS.gene053169	MS.gene76535	0.837201	5.98E-57	-1.69E-46
MS.gene05316	MS.gene76535	0.833649	4.72E-56	-1.69E-46
MS.gene053449	MS.gene76535	0.859954	2.89E-63	-1.69E-46
MS.gene053758	MS.gene76535	0.854441	1.23E-61	-1.69E-46
MS.gene053813	MS.gene76535	0.859002	5.59E-63	-1.69E-46
MS.gene053912	MS.gene76535	0.823477	1.36E-53	-1.69E-46
MS.gene054123	MS.gene76535	0.808941	2.42E-50	-1.69E-46
MS.gene054183	MS.gene76535	0.800565	1.36E-48	-1.69E-46
MS.gene054349	MS.gene76535	0.830841	2.34E-55	-1.69E-46
MS.gene054556	MS.gene76535	0.834792	2.44E-56	-1.69E-46
MS.gene054955	MS.gene76535	0.801959	7.04E-49	-1.69E-46
MS.gene05505	MS.gene76535	0.826465	2.67E-54	-1.69E-46
MS.gene055116	MS.gene76535	0.858505	7.87E-63	-1.69E-46
MS.gene055369	MS.gene76535	0.802081	6.64E-49	-1.69E-46
MS.gene055741	MS.gene76535	0.815355	9.65E-52	-1.69E-46
MS.gene055744	MS.gene76535	0.820787	5.70E-53	-1.69E-46
MS.gene055909	MS.gene76535	0.893354	6.79E-75	-1.69E-46
MS.gene055911	MS.gene76535	0.896326	4.11E-76	-1.69E-46
MS.gene056407	MS.gene76535	0.805189	1.51E-49	-1.69E-46
MS.gene056422	MS.gene76535	0.85599	4.35E-62	-1.69E-46
MS.gene056511	MS.gene76535	0.882683	8.48E-71	-1.69E-46
MS.gene05664	MS.gene76535	0.821522	3.86E-53	-1.69E-46
MS.gene05666	MS.gene76535	0.809418	1.91E-50	-1.69E-46
MS.gene057019	MS.gene76535	0.825131	5.54E-54	-1.69E-46
MS.gene057124	MS.gene76535	0.810925	9.04E-51	-1.69E-46
MS.gene057396	MS.gene76535	0.829306	5.54E-55	-1.69E-46
MS.gene057448	MS.gene76535	0.837215	5.93E-57	-1.69E-46
MS.gene057449	MS.gene76535	0.837856	4.06E-57	-1.69E-46
MS.gene057450	MS.gene76535	0.836398	9.58E-57	-1.69E-46
MS.gene057537	MS.gene76535	0.812403	4.32E-51	-1.69E-46
MS.gene057610	MS.gene76535	0.826098	3.27E-54	-1.69E-46
MS.gene057734	MS.gene76535	0.845394	4.18E-59	-1.69E-46
MS.gene057749	MS.gene76535	0.848012	8.04E-60	-1.69E-46
MS.gene057823	MS.gene76535	0.858779	6.52E-63	-1.69E-46
MS.gene057825	MS.gene76535	0.800819	1.20E-48	-1.69E-46
MS.gene058007	MS.gene76535	0.873482	1.42E-67	-1.69E-46
MS.gene058072	MS.gene76535	0.818056	2.39E-52	-1.69E-46
MS.gene058075	MS.gene76535	0.847246	1.31E-59	-1.69E-46
MS.gene058120	MS.gene76535	0.811736	6.03E-51	-1.69E-46
MS.gene058700	MS.gene76535	0.830045	3.66E-55	-1.69E-46
MS.gene058928	MS.gene76535	0.852698	3.89E-61	-1.69E-46
MS.gene058948	MS.gene76535	0.817045	4.04E-52	-1.69E-46
MS.gene058949	MS.gene76535	0.825411	4.76E-54	-1.69E-46
MS.gene058995	MS.gene76535	0.838036	3.65E-57	-1.69E-46
MS.gene05907	MS.gene76535	0.820234	7.64E-53	-1.69E-46
MS.gene059080	MS.gene76535	0.824952	6.11E-54	-1.69E-46
MS.gene059082	MS.gene76535	0.832105	1.14E-55	-1.69E-46
MS.gene059294	MS.gene76535	0.823945	1.05E-53	-1.69E-46
MS.gene059366	MS.gene76535	0.856297	3.54E-62	-1.69E-46
MS.gene059368	MS.gene76535	0.820603	6.29E-53	-1.69E-46
MS.gene059420	MS.gene76535	0.850859	1.29E-60	-1.69E-46
MS.gene059455	MS.gene76535	0.807696	4.46E-50	-1.69E-46
MS.gene059462	MS.gene76535	0.849545	3.02E-60	-1.69E-46
MS.gene059674	MS.gene76535	0.894529	2.26E-75	-1.69E-46
MS.gene059676	MS.gene76535	0.809845	1.55E-50	-1.69E-46
MS.gene059688	MS.gene76535	0.855417	6.40E-62	-1.69E-46
MS.gene059692	MS.gene76535	0.839338	1.68E-57	-1.69E-46
MS.gene059695	MS.gene76535	0.840478	8.49E-58	-1.69E-46
MS.gene059711	MS.gene76535	0.8367	8.02E-57	-1.69E-46
MS.gene059807	MS.gene76535	0.914127	2.76E-84	-1.69E-46
MS.gene059876	MS.gene76535	0.844511	7.23E-59	-1.69E-46
MS.gene059933	MS.gene76535	0.830656	2.60E-55	-1.69E-46
MS.gene060372	MS.gene76535	0.83742	5.25E-57	-1.69E-46
MS.gene060403	MS.gene76535	0.839014	2.04E-57	-1.69E-46
MS.gene060495	MS.gene76535	0.802163	6.39E-49	-1.69E-46
MS.gene060837	MS.gene76535	0.865423	5.97E-65	-1.69E-46
MS.gene06097	MS.gene76535	0.810434	1.15E-50	-1.69E-46
MS.gene061154	MS.gene76535	0.846474	2.12E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76535.t1	MTR_2g101140	98.980	196	2	0	1	196	1	196	3.08e-145	402
MS.gene76535.t1	MTR_8g013990	67.839	199	54	5	1	196	3	194	6.77e-92	267
MS.gene76535.t1	MTR_8g021190	42.969	128	69	3	61	186	29	154	2.34e-29	106
MS.gene76535.t1	MTR_1g103550	47.191	89	41	2	62	148	2	86	2.72e-22	87.8
MS.gene76535.t1	MTR_5g006430	38.053	113	65	2	62	173	2	110	1.50e-20	82.8
MS.gene76535.t1	MTR_3g105340	52.778	72	33	1	52	122	1	72	2.76e-20	82.8
MS.gene76535.t1	MTR_3g109860	34.959	123	77	3	32	151	4	126	1.51e-17	75.9
MS.gene76535.t1	MTR_4g125010	50.725	69	33	1	61	128	23	91	6.19e-17	74.7
MS.gene76535.t1	MTR_4g125030	42.708	96	47	3	61	151	21	113	3.68e-16	71.6
MS.gene76535.t1	MTR_1g014930	41.111	90	39	3	53	135	4	86	2.21e-15	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76535.t1	AT5G25840	50.276	181	59	4	36	196	4	173	3.66e-53	168
MS.gene76535.t1	AT1G79770	53.205	156	59	3	35	190	26	167	1.23e-48	156
MS.gene76535.t1	AT1G72510	33.537	164	90	5	39	186	5	165	8.76e-23	90.1
MS.gene76535.t1	AT1G72510	33.537	164	90	5	39	186	5	165	8.76e-23	90.1
MS.gene76535.t1	AT4G14819	40.777	103	50	3	62	164	2	93	5.28e-22	86.3
MS.gene76535.t1	AT3G22540	40.449	89	50	1	62	150	2	87	1.92e-21	85.1
MS.gene76535.t1	AT2G25780	36.250	160	88	5	31	185	1	151	1.10e-20	84.3
MS.gene76535.t1	AT2G09970	31.707	164	92	5	40	186	6	166	1.23e-20	84.7
MS.gene76535.t1	AT1G54095	37.410	139	75	4	61	189	14	150	5.47e-20	82.4
MS.gene76535.t1	AT5G20670	33.846	130	80	3	63	186	23	152	3.57e-18	77.8

Find 34 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGTTAGATTTAATAAATT+TGG	0.209428	2.3:+2762853	MS.gene76535:CDS
AGCTTCTATATAAGATTAAT+TGG	0.236977	2.3:-2763064	None:intergenic
GCATCTTCAACTTCAGTTAT+CGG	0.334901	2.3:-2762655	None:intergenic
GTGATAAATACTTAGGGAAA+TGG	0.349825	2.3:+2762737	MS.gene76535:CDS
AGTTGAGATGAATTAGGTTT+TGG	0.358947	2.3:-2762550	None:intergenic
GCTTCAGCTTGAAACAAAAC+AGG	0.390939	2.3:-2762886	None:intergenic
CGTGTACGTGATAAATACTT+AGG	0.397786	2.3:+2762730	MS.gene76535:CDS
TTGTTGAGTTGAGATGAATT+AGG	0.428907	2.3:-2762556	None:intergenic
TTTCACCACCTTTGTCTCTA+GGG	0.429554	2.3:-2762966	None:intergenic
TGATAAATACTTAGGGAAAT+GGG	0.445526	2.3:+2762738	MS.gene76535:CDS
TGAGGCAGTGAAAGAAGAGT+TGG	0.465910	2.3:+2762777	MS.gene76535:CDS
TTTGGAGCATACAATGTTGT+TGG	0.466523	2.3:-2762524	None:intergenic
TACTTAGGGAAATGGGTTTG+TGG	0.466697	2.3:+2762745	MS.gene76535:CDS
GAGTAAAATGGAAGGGAGAA+GGG	0.485393	2.3:+2762930	MS.gene76535:CDS
TTGATATCTCTTGTAATAGC+AGG	0.500356	2.3:-2763027	None:intergenic
AGAGTAAAATGGAAGGGAGA+AGG	0.512738	2.3:+2762929	MS.gene76535:CDS
GTGTACGTGATAAATACTTA+GGG	0.514569	2.3:+2762731	MS.gene76535:CDS
TCAACTATTATGGCACCTCA+TGG	0.537712	2.3:+2762472	None:intergenic
GTGGAAGCTGTGTTAAATCA+AGG	0.538899	2.3:-2762633	None:intergenic
GGTGGTGAAAAGAAAGGTGG+TGG	0.545436	2.3:+2762979	MS.gene76535:CDS
TTAGATTTAATAAATTTGGA+AGG	0.546403	2.3:+2762857	MS.gene76535:CDS
CATTACCTAGCTCAATCTCT+AGG	0.548665	2.3:+2762500	MS.gene76535:CDS
GACAAAGGTGGTGAAAAGAA+AGG	0.558067	2.3:+2762973	MS.gene76535:CDS
TTGGTCCTAGAGATTGAGCT+AGG	0.570237	2.3:-2762505	None:intergenic
GGTTTGTGGTTTGTGTTCTG+AGG	0.597680	2.3:+2762759	MS.gene76535:CDS
TGGTGGGGAAAATATTGAAG+AGG	0.600750	2.3:+2762807	MS.gene76535:CDS
TCTTCAACTTCAGTTATCGG+TGG	0.626778	2.3:-2762652	None:intergenic
TCATTCAACCCTAGAGACAA+AGG	0.638410	2.3:+2762958	MS.gene76535:CDS
CTTCAGCTTGAAACAAAACA+GGG	0.640341	2.3:-2762885	None:intergenic
GTGGTGAAAAGAAAGGTGGT+GGG	0.641104	2.3:+2762980	MS.gene76535:CDS
AAAGGTGGTGAAAAGAAAGG+TGG	0.652842	2.3:+2762976	MS.gene76535:CDS
GATGCAAAGTGTGAATGTTG+TGG	0.690241	2.3:+2762673	MS.gene76535:CDS
TTCAACCCTAGAGACAAAGG+TGG	0.741656	2.3:+2762961	MS.gene76535:CDS
CTAGGTAATGAATCTCCATG+AGG	0.751297	2.3:-2762487	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTGTTAGATTTAATAAATT+TGG	+	chr2.3:2762853-2762872	MS.gene76535:CDS	15.0%
!!	TTAGATTTAATAAATTTGGA+AGG	+	chr2.3:2762857-2762876	MS.gene76535:CDS	15.0%
!	TGAAAAAGAGTAAAATGGAA+GGG	+	chr2.3:2762923-2762942	MS.gene76535:CDS	25.0%
!	TGATAAATACTTAGGGAAAT+GGG	+	chr2.3:2762738-2762757	MS.gene76535:CDS	25.0%
	AAAGAAGAGTTGGAGAAAAA+TGG	+	chr2.3:2762787-2762806	MS.gene76535:CDS	30.0%
	CTGAAAAAGAGTAAAATGGA+AGG	+	chr2.3:2762922-2762941	MS.gene76535:CDS	30.0%
	GATGCTGAAAAAGAGTAAAA+TGG	+	chr2.3:2762918-2762937	MS.gene76535:CDS	30.0%
	GTGATAAATACTTAGGGAAA+TGG	+	chr2.3:2762737-2762756	MS.gene76535:CDS	30.0%
	GTGTACGTGATAAATACTTA+GGG	+	chr2.3:2762731-2762750	MS.gene76535:CDS	30.0%
	TTGATATCTCTTGTAATAGC+AGG	-	chr2.3:2763030-2763049	None:intergenic	30.0%
!	AGTTGAGATGAATTAGGTTT+TGG	-	chr2.3:2762553-2762572	None:intergenic	30.0%
!!	TTGTTGAGTTGAGATGAATT+AGG	-	chr2.3:2762559-2762578	None:intergenic	30.0%
	AAGAGTTGGAGAAAAATGGT+GGG	+	chr2.3:2762791-2762810	MS.gene76535:CDS	35.0%
	CGTGTACGTGATAAATACTT+AGG	+	chr2.3:2762730-2762749	MS.gene76535:CDS	35.0%
	CTTCAGCTTGAAACAAAACA+GGG	-	chr2.3:2762888-2762907	None:intergenic	35.0%
	GCATCTTCAACTTCAGTTAT+CGG	-	chr2.3:2762658-2762677	None:intergenic	35.0%
!	TTTGGAGCATACAATGTTGT+TGG	-	chr2.3:2762527-2762546	None:intergenic	35.0%
!!	TGAATTAGGTTTTGGTGGTT+TGG	-	chr2.3:2762545-2762564	None:intergenic	35.0%
!!	TGAGATGAATTAGGTTTTGG+TGG	-	chr2.3:2762550-2762569	None:intergenic	35.0%
	AAAGGTGGTGAAAAGAAAGG+TGG	+	chr2.3:2762976-2762995	MS.gene76535:CDS	40.0%
	AGAGTAAAATGGAAGGGAGA+AGG	+	chr2.3:2762929-2762948	MS.gene76535:CDS	40.0%
	AGAGTTGGAGAAAAATGGTG+GGG	+	chr2.3:2762792-2762811	MS.gene76535:CDS	40.0%
	CATTACCTAGCTCAATCTCT+AGG	+	chr2.3:2762500-2762519	MS.gene76535:CDS	40.0%
	CTAGGTAATGAATCTCCATG+AGG	-	chr2.3:2762490-2762509	None:intergenic	40.0%
	GAAGAGTTGGAGAAAAATGG+TGG	+	chr2.3:2762790-2762809	MS.gene76535:CDS	40.0%
	GACAAAGGTGGTGAAAAGAA+AGG	+	chr2.3:2762973-2762992	MS.gene76535:CDS	40.0%
	GAGTAAAATGGAAGGGAGAA+GGG	+	chr2.3:2762930-2762949	MS.gene76535:CDS	40.0%
	GATGCAAAGTGTGAATGTTG+TGG	+	chr2.3:2762673-2762692	MS.gene76535:CDS	40.0%
	GCTTCAGCTTGAAACAAAAC+AGG	-	chr2.3:2762889-2762908	None:intergenic	40.0%
	GTGGAAGCTGTGTTAAATCA+AGG	-	chr2.3:2762636-2762655	None:intergenic	40.0%
	TCATTCAACCCTAGAGACAA+AGG	+	chr2.3:2762958-2762977	MS.gene76535:CDS	40.0%
	TCTTCAACTTCAGTTATCGG+TGG	-	chr2.3:2762655-2762674	None:intergenic	40.0%
	TGGTGGGGAAAATATTGAAG+AGG	+	chr2.3:2762807-2762826	MS.gene76535:CDS	40.0%
	TTTCACCACCTTTGTCTCTA+GGG	-	chr2.3:2762969-2762988	None:intergenic	40.0%
!	CGTACACGTTTTATGTACTC+AGG	-	chr2.3:2762718-2762737	None:intergenic	40.0%
!	TACTTAGGGAAATGGGTTTG+TGG	+	chr2.3:2762745-2762764	MS.gene76535:CDS	40.0%
!	TTTTCACCACCTTTGTCTCT+AGG	-	chr2.3:2762970-2762989	None:intergenic	40.0%
	GTGGTGAAAAGAAAGGTGGT+GGG	+	chr2.3:2762980-2762999	MS.gene76535:CDS	45.0%
	TGAGGCAGTGAAAGAAGAGT+TGG	+	chr2.3:2762777-2762796	MS.gene76535:CDS	45.0%
	TTCAACCCTAGAGACAAAGG+TGG	+	chr2.3:2762961-2762980	MS.gene76535:CDS	45.0%
	TTGGTCCTAGAGATTGAGCT+AGG	-	chr2.3:2762508-2762527	None:intergenic	45.0%
!	GGTTTGTGGTTTGTGTTCTG+AGG	+	chr2.3:2762759-2762778	MS.gene76535:CDS	45.0%
	GGTGGTGAAAAGAAAGGTGG+TGG	+	chr2.3:2762979-2762998	MS.gene76535:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	2762481	2763071	2762481	ID=MS.gene76535
chr2.3	mRNA	2762481	2763071	2762481	ID=MS.gene76535.t1;Parent=MS.gene76535
chr2.3	exon	2762481	2763071	2762481	ID=MS.gene76535.t1.exon1;Parent=MS.gene76535.t1
chr2.3	CDS	2762481	2763071	2762481	ID=cds.MS.gene76535.t1;Parent=MS.gene76535.t1

Gene Sequence

>MS.gene76535

ATGGCACCTCATGGAGATTCATTACCTAGCTCAATCTCTAGGACCAACAACATTGTATGCTCCAAACCACCAAAACCTAATTCATCTCAACTCAACAACAATAATATTCAAAGAACAATCTCAGACATATCATTTGAACTATCAAAACAAGACCTTGATTTAACACAGCTTCCACCGATAACTGAAGTTGAAGATGCAAAGTGTGAATGTTGTGGCATGTCTGAAGAGTTTACACCTGAGTACATAAAACGTGTACGTGATAAATACTTAGGGAAATGGGTTTGTGGTTTGTGTTCTGAGGCAGTGAAAGAAGAGTTGGAGAAAAATGGTGGGGAAAATATTGAAGAGGCTTTGAGTGCACACATGAGTGCATGTGTTAGATTTAATAAATTTGGAAGGAGTTTCCCTGTTTTGTTTCAAGCTGAAGCTATGAAAGAGATGCTGAAAAAGAGTAAAATGGAAGGGAGAAGGGCTAAGTCATTCAACCCTAGAGACAAAGGTGGTGAAAAGAAAGGTGGTGGGCTTGCTAGAAGTTCAAGTTGCATTCCTGCTATTACAAGAGATATCAATGATTTCACAATCACCAATTAA

Protein sequence

>MS.gene76535.t1

MAPHGDSLPSSISRTNNIVCSKPPKPNSSQLNNNNIQRTISDISFELSKQDLDLTQLPPITEVEDAKCECCGMSEEFTPEYIKRVRDKYLGKWVCGLCSEAVKEELEKNGGENIEEALSAHMSACVRFNKFGRSFPVLFQAEAMKEMLKKSKMEGRRAKSFNPRDKGGEKKGGGLARSSSCIPAITRDINDFTITN