Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061879.t1 | XP_003625486.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.70E-21 | 111.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061879.t1 | Q2HT99 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.0e-21 | 111.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene061879 | 0.837465 | 5.11E-57 | -1.69E-46 |
| MS.gene050584 | MS.gene061879 | 0.832983 | 6.92E-56 | -1.69E-46 |
| MS.gene050585 | MS.gene061879 | 0.832885 | 7.32E-56 | -1.69E-46 |
| MS.gene050586 | MS.gene061879 | 0.800464 | 1.42E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene061879 | 0.829504 | 4.96E-55 | -1.69E-46 |
| MS.gene050808 | MS.gene061879 | 0.818669 | 1.74E-52 | -1.69E-46 |
| MS.gene051626 | MS.gene061879 | 0.80426 | 2.35E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene061879 | 0.875209 | 3.69E-68 | -1.69E-46 |
| MS.gene052142 | MS.gene061879 | 0.818125 | 2.31E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene061879 | 0.848392 | 6.31E-60 | -1.69E-46 |
| MS.gene053383 | MS.gene061879 | 0.804116 | 2.52E-49 | -1.69E-46 |
| MS.gene053563 | MS.gene061879 | 0.808839 | 2.54E-50 | -1.69E-46 |
| MS.gene05456 | MS.gene061879 | 0.836937 | 6.98E-57 | -1.69E-46 |
| MS.gene054596 | MS.gene061879 | 0.807247 | 5.55E-50 | -1.69E-46 |
| MS.gene055207 | MS.gene061879 | 0.805813 | 1.11E-49 | -1.69E-46 |
| MS.gene055359 | MS.gene061879 | 0.812219 | 4.73E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene061879 | 0.842588 | 2.36E-58 | -1.69E-46 |
| MS.gene059006 | MS.gene061879 | 0.865411 | 6.02E-65 | -1.69E-46 |
| MS.gene059124 | MS.gene061879 | 0.806968 | 6.36E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061879.t1 | MTR_7g099670 | 98.077 | 104 | 2 | 0 | 1 | 104 | 1 | 104 | 3.16e-60 | 179 |
| MS.gene061879.t1 | MTR_1g069085 | 68.000 | 50 | 14 | 2 | 1 | 49 | 1 | 49 | 5.67e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGGTGGACTTGGTGGTTT+GGG | 0.209445 | 7.4:-10553436 | MS.gene061879:CDS |
| ACATTTGCTGGTGGTTATTC+AGG | 0.231052 | 7.4:-10553532 | MS.gene061879:CDS |
| GGAGGACTTGGTGGGGTTGG+TGG | 0.235350 | 7.4:-10553415 | MS.gene061879:CDS |
| CCTAGGTGGACTTGGTGGTT+TGG | 0.244833 | 7.4:-10553437 | MS.gene061879:CDS |
| CTTGGTGGTTTGGGAGGACT+TGG | 0.272357 | 7.4:-10553427 | MS.gene061879:CDS |
| GGACTTGGTGGGGTTGGTGG+TGG | 0.293034 | 7.4:-10553412 | MS.gene061879:CDS |
| GGTGGAGCTGGTGGTGGTGT+TGG | 0.293326 | 7.4:-10553394 | MS.gene061879:CDS |
| GGTGGAATCGGTGGTGATTT+AGG | 0.312961 | 7.4:-10553472 | MS.gene061879:CDS |
| GGTGGTTTGGGAGGACTTGG+TGG | 0.314590 | 7.4:-10553424 | MS.gene061879:CDS |
| TTGGGAGGACTTGGTGGGGT+TGG | 0.314725 | 7.4:-10553418 | MS.gene061879:CDS |
| GGTGGGGTTGGTGGTGGAGC+TGG | 0.316689 | 7.4:-10553406 | MS.gene061879:CDS |
| GTTGGTGGTGGAGCTGGTGG+TGG | 0.321018 | 7.4:-10553400 | MS.gene061879:CDS |
| GGAAACAATGGACTTCCTTT+TGG | 0.322482 | 7.4:-10553505 | MS.gene061879:CDS |
| TTAGGTGGTGGTGGTGGCCT+AGG | 0.323708 | 7.4:-10553454 | MS.gene061879:CDS |
| GGTGGTGGCCTAGGTGGACT+TGG | 0.331166 | 7.4:-10553445 | MS.gene061879:CDS |
| GGTGGTGTTGGAGGTGGTGT+TGG | 0.331290 | 7.4:-10553382 | MS.gene061879:CDS |
| GGGGTTGGTGGTGGAGCTGG+TGG | 0.333006 | 7.4:-10553403 | MS.gene061879:CDS |
| GCTGGTGGTTATTCAGGAAT+AGG | 0.341133 | 7.4:-10553526 | MS.gene061879:CDS |
| GTTGGAGGTGGTGTTGGAAC+TGG | 0.344227 | 7.4:-10553376 | MS.gene061879:CDS |
| CAAATGTCATGAAGTTCTTC+TGG | 0.354882 | 7.4:+10553548 | None:intergenic |
| GCTGGTGGTGGTGTTGGAGG+TGG | 0.373109 | 7.4:-10553388 | MS.gene061879:CDS |
| AAGAACTTCATGACATTTGC+TGG | 0.375624 | 7.4:-10553544 | MS.gene061879:CDS |
| GGAGCTGGTGGTGGTGTTGG+AGG | 0.379910 | 7.4:-10553391 | MS.gene061879:CDS |
| GGTGGTGTTGGAACTGGTGT+TGG | 0.385912 | 7.4:-10553370 | MS.gene061879:CDS |
| GGTGGAATTGGAGGTGGAAT+CGG | 0.386910 | 7.4:-10553484 | MS.gene061879:CDS |
| GTGGTTTGGGAGGACTTGGT+GGG | 0.431797 | 7.4:-10553423 | MS.gene061879:CDS |
| TGGTTTGGGAGGACTTGGTG+GGG | 0.448017 | 7.4:-10553422 | MS.gene061879:CDS |
| GGTGTTGGAACTGGTGTTGG+TGG | 0.458137 | 7.4:-10553367 | MS.gene061879:CDS |
| AAAGTGGTGTATGTTGTTGT+TGG | 0.463389 | 7.4:-10553635 | MS.gene061879:CDS |
| GGTGGCCTAGGTGGACTTGG+TGG | 0.480801 | 7.4:-10553442 | MS.gene061879:CDS |
| GGTGGACTTGGTGGTTTGGG+AGG | 0.508576 | 7.4:-10553433 | MS.gene061879:CDS |
| GTTGGTTCTTGCTCTTGTTG+TGG | 0.516173 | 7.4:-10553617 | MS.gene061879:CDS |
| GGTGGTGGTGGTGGCCTAGG+TGG | 0.516203 | 7.4:-10553451 | MS.gene061879:CDS |
| ACCAACTGATGCTGGTTTGA+AGG | 0.518003 | 7.4:-10553572 | MS.gene061879:CDS |
| GGTGATTTAGGTGGTGGTGG+TGG | 0.527759 | 7.4:-10553460 | MS.gene061879:CDS |
| ATCGGTGGTGATTTAGGTGG+TGG | 0.527781 | 7.4:-10553466 | MS.gene061879:CDS |
| CCACCTCCAATTCCACCAAA+AGG | 0.542402 | 7.4:+10553490 | None:intergenic |
| GGAATTGGAGGTGGAATCGG+TGG | 0.552447 | 7.4:-10553481 | MS.gene061879:CDS |
| GGTGGTGATTTAGGTGGTGG+TGG | 0.559090 | 7.4:-10553463 | MS.gene061879:CDS |
| GAATACTGATCTTAAGGGAA+AGG | 0.564229 | 7.4:+10553337 | None:intergenic |
| AGAAATGTACCAACTGATGC+TGG | 0.575990 | 7.4:-10553580 | MS.gene061879:CDS |
| TATTCAGGAATAGGAAACAA+TGG | 0.605549 | 7.4:-10553517 | MS.gene061879:CDS |
| GGAATCGGTGGTGATTTAGG+TGG | 0.612801 | 7.4:-10553469 | MS.gene061879:CDS |
| GAGTGAGAGATATGGCAAAG+TGG | 0.614546 | 7.4:-10553651 | None:intergenic |
| AACTTCATGACATTTGCTGG+TGG | 0.621619 | 7.4:-10553541 | MS.gene061879:CDS |
| TCCTTCAAACCAGCATCAGT+TGG | 0.622405 | 7.4:+10553571 | None:intergenic |
| CCAAACCACCAAGTCCACCT+AGG | 0.637542 | 7.4:+10553437 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTCAGGAATAGGAAACAA+TGG | - | chr7.4:10553471-10553490 | MS.gene061879:CDS | 30.0% | |
| AAAGTGGTGTATGTTGTTGT+TGG | - | chr7.4:10553353-10553372 | MS.gene061879:CDS | 35.0% | |
| AAGAACTTCATGACATTTGC+TGG | - | chr7.4:10553444-10553463 | MS.gene061879:CDS | 35.0% | |
| CAAATGTCATGAAGTTCTTC+TGG | + | chr7.4:10553443-10553462 | None:intergenic | 35.0% | |
| AGAAATGTACCAACTGATGC+TGG | - | chr7.4:10553408-10553427 | MS.gene061879:CDS | 40.0% | |
| ! | AACTTCATGACATTTGCTGG+TGG | - | chr7.4:10553447-10553466 | MS.gene061879:CDS | 40.0% |
| ! | ACATTTGCTGGTGGTTATTC+AGG | - | chr7.4:10553456-10553475 | MS.gene061879:CDS | 40.0% |
| !! | AACAATGGACTTCCTTTTGG+TGG | - | chr7.4:10553486-10553505 | MS.gene061879:CDS | 40.0% |
| !! | GGAAACAATGGACTTCCTTT+TGG | - | chr7.4:10553483-10553502 | MS.gene061879:CDS | 40.0% |
| GCTGGTGGTTATTCAGGAAT+AGG | - | chr7.4:10553462-10553481 | MS.gene061879:CDS | 45.0% | |
| TCCTTCAAACCAGCATCAGT+TGG | + | chr7.4:10553420-10553439 | None:intergenic | 45.0% | |
| ! | CTTCCTTTTGGTGGAATTGG+AGG | - | chr7.4:10553495-10553514 | MS.gene061879:CDS | 45.0% |
| ! | GGACTTCCTTTTGGTGGAAT+TGG | - | chr7.4:10553492-10553511 | MS.gene061879:CDS | 45.0% |
| ! | GTTGGTTCTTGCTCTTGTTG+TGG | - | chr7.4:10553371-10553390 | MS.gene061879:CDS | 45.0% |
| !! | ACCAACTGATGCTGGTTTGA+AGG | - | chr7.4:10553416-10553435 | MS.gene061879:CDS | 45.0% |
| ATCGGTGGTGATTTAGGTGG+TGG | - | chr7.4:10553522-10553541 | MS.gene061879:CDS | 50.0% | |
| CCACCTCCAATTCCACCAAA+AGG | + | chr7.4:10553501-10553520 | None:intergenic | 50.0% | |
| GGAATCGGTGGTGATTTAGG+TGG | - | chr7.4:10553519-10553538 | MS.gene061879:CDS | 50.0% | |
| GGTGGAATCGGTGGTGATTT+AGG | - | chr7.4:10553516-10553535 | MS.gene061879:CDS | 50.0% | |
| GGTGGAATTGGAGGTGGAAT+CGG | - | chr7.4:10553504-10553523 | MS.gene061879:CDS | 50.0% | |
| ! | CCTTTTGGTGGAATTGGAGG+TGG | - | chr7.4:10553498-10553517 | MS.gene061879:CDS | 50.0% |
| !! | CTAGGTGGACTTGGTGGTTT+GGG | - | chr7.4:10553552-10553571 | MS.gene061879:CDS | 50.0% |
| CCAAACCACCAAGTCCACCT+AGG | + | chr7.4:10553554-10553573 | None:intergenic | 55.0% | |
| GGAATTGGAGGTGGAATCGG+TGG | - | chr7.4:10553507-10553526 | MS.gene061879:CDS | 55.0% | |
| ! | CTTGGTGGTTTGGGAGGACT+TGG | - | chr7.4:10553561-10553580 | MS.gene061879:CDS | 55.0% |
| ! | GGTGGTGATTTAGGTGGTGG+TGG | - | chr7.4:10553525-10553544 | MS.gene061879:CDS | 55.0% |
| ! | GGTGGTGTTGGAACTGGTGT+TGG | - | chr7.4:10553618-10553637 | MS.gene061879:CDS | 55.0% |
| ! | GGTGTTGGAACTGGTGTTGG+TGG | - | chr7.4:10553621-10553640 | MS.gene061879:CDS | 55.0% |
| ! | GTGGTTTGGGAGGACTTGGT+GGG | - | chr7.4:10553565-10553584 | MS.gene061879:CDS | 55.0% |
| ! | GTTGGAGGTGGTGTTGGAAC+TGG | - | chr7.4:10553612-10553631 | MS.gene061879:CDS | 55.0% |
| !! | CCTAGGTGGACTTGGTGGTT+TGG | - | chr7.4:10553551-10553570 | MS.gene061879:CDS | 55.0% |
| !! | GGTGATTTAGGTGGTGGTGG+TGG | - | chr7.4:10553528-10553547 | MS.gene061879:CDS | 55.0% |
| !! | TGGTTTGGGAGGACTTGGTG+GGG | - | chr7.4:10553566-10553585 | MS.gene061879:CDS | 55.0% |
| ! | GGTGGTGTTGGAGGTGGTGT+TGG | - | chr7.4:10553606-10553625 | MS.gene061879:CDS | 60.0% |
| ! | GGTGGTTTGGGAGGACTTGG+TGG | - | chr7.4:10553564-10553583 | MS.gene061879:CDS | 60.0% |
| !! | GGTGGACTTGGTGGTTTGGG+AGG | - | chr7.4:10553555-10553574 | MS.gene061879:CDS | 60.0% |
| !! | TTAGGTGGTGGTGGTGGCCT+AGG | - | chr7.4:10553534-10553553 | MS.gene061879:CDS | 60.0% |
| !! | TTGGGAGGACTTGGTGGGGT+TGG | - | chr7.4:10553570-10553589 | MS.gene061879:CDS | 60.0% |
| ! | GGTGGCCTAGGTGGACTTGG+TGG | - | chr7.4:10553546-10553565 | MS.gene061879:CDS | 65.0% |
| ! | GGTGGTGGCCTAGGTGGACT+TGG | - | chr7.4:10553543-10553562 | MS.gene061879:CDS | 65.0% |
| !! | GCTGGTGGTGGTGTTGGAGG+TGG | - | chr7.4:10553600-10553619 | MS.gene061879:CDS | 65.0% |
| !! | GGACTTGGTGGGGTTGGTGG+TGG | - | chr7.4:10553576-10553595 | MS.gene061879:CDS | 65.0% |
| !! | GGAGCTGGTGGTGGTGTTGG+AGG | - | chr7.4:10553597-10553616 | MS.gene061879:CDS | 65.0% |
| !! | GGAGGACTTGGTGGGGTTGG+TGG | - | chr7.4:10553573-10553592 | MS.gene061879:CDS | 65.0% |
| !! | GGTGGAGCTGGTGGTGGTGT+TGG | - | chr7.4:10553594-10553613 | MS.gene061879:CDS | 65.0% |
| !! | GTTGGTGGTGGAGCTGGTGG+TGG | - | chr7.4:10553588-10553607 | MS.gene061879:CDS | 65.0% |
| !! | GGGGTTGGTGGTGGAGCTGG+TGG | - | chr7.4:10553585-10553604 | MS.gene061879:CDS | 70.0% |
| !! | GGTGGGGTTGGTGGTGGAGC+TGG | - | chr7.4:10553582-10553601 | MS.gene061879:CDS | 70.0% |
| !! | GGTGGTGGTGGTGGCCTAGG+TGG | - | chr7.4:10553537-10553556 | MS.gene061879:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10553348 | 10553662 | 10553348 | ID=MS.gene061879 |
| chr7.4 | mRNA | 10553348 | 10553662 | 10553348 | ID=MS.gene061879.t1;Parent=MS.gene061879 |
| chr7.4 | exon | 10553348 | 10553662 | 10553348 | ID=MS.gene061879.t1.exon1;Parent=MS.gene061879.t1 |
| chr7.4 | CDS | 10553348 | 10553662 | 10553348 | ID=cds.MS.gene061879.t1;Parent=MS.gene061879.t1 |
| Gene Sequence |
| Protein sequence |
