Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063782.t1 | XP_003627923.2 | 95.6 | 113 | 5 | 0 | 1 | 113 | 8 | 120 | 8.50E-53 | 216.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063782.t1 | G7LGV9 | 95.6 | 113 | 5 | 0 | 1 | 113 | 6 | 118 | 6.1e-53 | 216.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene063782 | 0.822276 | 2.58E-53 | -1.69E-46 |
| MS.gene050363 | MS.gene063782 | 0.816827 | 4.52E-52 | -1.69E-46 |
| MS.gene050381 | MS.gene063782 | 0.804048 | 2.60E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene063782 | 0.81609 | 6.61E-52 | -1.69E-46 |
| MS.gene051946 | MS.gene063782 | 0.816316 | 5.89E-52 | -1.69E-46 |
| MS.gene052091 | MS.gene063782 | 0.803396 | 3.56E-49 | -1.69E-46 |
| MS.gene053549 | MS.gene063782 | 0.820861 | 5.49E-53 | -1.69E-46 |
| MS.gene055549 | MS.gene063782 | 0.831091 | 2.03E-55 | -1.69E-46 |
| MS.gene055550 | MS.gene063782 | 0.827277 | 1.71E-54 | -1.69E-46 |
| MS.gene055551 | MS.gene063782 | 0.807964 | 3.91E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene063782 | 0.80782 | 4.19E-50 | -1.69E-46 |
| MS.gene056311 | MS.gene063782 | 0.845057 | 5.15E-59 | -1.69E-46 |
| MS.gene056408 | MS.gene063782 | 0.804181 | 2.44E-49 | -1.69E-46 |
| MS.gene056530 | MS.gene063782 | 0.834594 | 2.74E-56 | -1.69E-46 |
| MS.gene057014 | MS.gene063782 | 0.822445 | 2.36E-53 | -1.69E-46 |
| MS.gene058493 | MS.gene063782 | 0.82746 | 1.55E-54 | -1.69E-46 |
| MS.gene058494 | MS.gene063782 | 0.816436 | 5.54E-52 | -1.69E-46 |
| MS.gene05884 | MS.gene063782 | 0.824171 | 9.33E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063782.t1 | MTR_8g040080 | 95.575 | 113 | 5 | 0 | 1 | 113 | 6 | 118 | 3.60e-78 | 226 |
| MS.gene063782.t1 | MTR_3g013980 | 48.649 | 111 | 47 | 3 | 8 | 112 | 21 | 127 | 9.05e-24 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGATGAAAGAGTGAATA+AGG | 0.308800 | 8.1:-63995204 | MS.gene063782:CDS |
| GAAAGAAACTCACTGTAATC+AGG | 0.334978 | 8.1:+63995116 | None:intergenic |
| TATTGAACGTGTTCACTTAA+CGG | 0.366611 | 8.1:+63995074 | None:intergenic |
| GAACAAAAGGATGAGCAAAA+AGG | 0.402666 | 8.1:-63995375 | None:intergenic |
| GTTGAGCTTTGATATGTCCT+AGG | 0.406060 | 8.1:-63995045 | None:intergenic |
| GTCCAGTTAGTTCTTGTACA+AGG | 0.425814 | 8.1:+63995279 | None:intergenic |
| GCCCTTGTACAAGAACTAAC+TGG | 0.427191 | 8.1:-63995281 | MS.gene063782:CDS |
| TCCAGTTAGTTCTTGTACAA+GGG | 0.449158 | 8.1:+63995280 | None:intergenic |
| TCTTTCAAGACAATCCTTTG+TGG | 0.449375 | 8.1:-63995099 | MS.gene063782:CDS |
| GCATTAGTCTTCACCTTCAC+AGG | 0.454448 | 8.1:+63995317 | None:intergenic |
| ACTGTAATCAGGTTTCAAAA+AGG | 0.461670 | 8.1:+63995127 | None:intergenic |
| TTGAGCTTTGATATGTCCTA+GGG | 0.482288 | 8.1:-63995044 | None:intergenic |
| AGTAGTAGTTTCAGCAACAT+TGG | 0.513923 | 8.1:+63995250 | None:intergenic |
| TTCAACTCATCAACAATGGA+GGG | 0.573923 | 8.1:-63995174 | MS.gene063782:CDS |
| TGAAACTACTACTATGCCCT+TGG | 0.576114 | 8.1:-63995240 | MS.gene063782:CDS |
| GATGATGAAAGAGTGAATAA+GGG | 0.580204 | 8.1:-63995203 | MS.gene063782:CDS |
| ATTCAACTCATCAACAATGG+AGG | 0.580916 | 8.1:-63995175 | MS.gene063782:CDS |
| ATATATTTCAAGTCCTGTGA+AGG | 0.593591 | 8.1:-63995330 | MS.gene063782:CDS |
| GTTATTCAACTCATCAACAA+TGG | 0.609525 | 8.1:-63995178 | MS.gene063782:CDS |
| TTCACTTAACGGCTCCACAA+AGG | 0.646003 | 8.1:+63995085 | None:intergenic |
| GATGAAAGAGTGAATAAGGG+TGG | 0.686509 | 8.1:-63995200 | MS.gene063782:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTGTAATCAGGTTTCAAAA+AGG | + | chr8.1:63995287-63995306 | None:intergenic | 30.0% | |
| GATGATGAAAGAGTGAATAA+GGG | - | chr8.1:63995208-63995227 | MS.gene063782:CDS | 30.0% | |
| GTTATTCAACTCATCAACAA+TGG | - | chr8.1:63995233-63995252 | MS.gene063782:CDS | 30.0% | |
| TATTGAACGTGTTCACTTAA+CGG | + | chr8.1:63995340-63995359 | None:intergenic | 30.0% | |
| ! | ATATATTTCAAGTCCTGTGA+AGG | - | chr8.1:63995081-63995100 | MS.gene063782:CDS | 30.0% |
| ! | ATCACAAAGTTTTTCTTCCA+AGG | + | chr8.1:63995191-63995210 | None:intergenic | 30.0% |
| ! | TCACAAAGTTTTTCTTCCAA+GGG | + | chr8.1:63995190-63995209 | None:intergenic | 30.0% |
| ! | TGATGATGAAAGAGTGAATA+AGG | - | chr8.1:63995207-63995226 | MS.gene063782:CDS | 30.0% |
| AGTAGTAGTTTCAGCAACAT+TGG | + | chr8.1:63995164-63995183 | None:intergenic | 35.0% | |
| ATTCAACTCATCAACAATGG+AGG | - | chr8.1:63995236-63995255 | MS.gene063782:CDS | 35.0% | |
| GAAAGAAACTCACTGTAATC+AGG | + | chr8.1:63995298-63995317 | None:intergenic | 35.0% | |
| TCTTTCAAGACAATCCTTTG+TGG | - | chr8.1:63995312-63995331 | MS.gene063782:CDS | 35.0% | |
| TTCAACTCATCAACAATGGA+GGG | - | chr8.1:63995237-63995256 | MS.gene063782:CDS | 35.0% | |
| ! | TCCAGTTAGTTCTTGTACAA+GGG | + | chr8.1:63995134-63995153 | None:intergenic | 35.0% |
| GATGAAAGAGTGAATAAGGG+TGG | - | chr8.1:63995211-63995230 | MS.gene063782:CDS | 40.0% | |
| TGAAACTACTACTATGCCCT+TGG | - | chr8.1:63995171-63995190 | MS.gene063782:CDS | 40.0% | |
| ! | GTCCAGTTAGTTCTTGTACA+AGG | + | chr8.1:63995135-63995154 | None:intergenic | 40.0% |
| GCCCTTGTACAAGAACTAAC+TGG | - | chr8.1:63995130-63995149 | MS.gene063782:CDS | 45.0% | |
| TTCACTTAACGGCTCCACAA+AGG | + | chr8.1:63995329-63995348 | None:intergenic | 45.0% | |
| !! | GCATTAGTCTTCACCTTCAC+AGG | + | chr8.1:63995097-63995116 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 63995046 | 63995387 | 63995046 | ID=MS.gene063782 |
| chr8.1 | mRNA | 63995046 | 63995387 | 63995046 | ID=MS.gene063782.t1;Parent=MS.gene063782 |
| chr8.1 | exon | 63995046 | 63995387 | 63995046 | ID=MS.gene063782.t1.exon1;Parent=MS.gene063782.t1 |
| chr8.1 | CDS | 63995046 | 63995387 | 63995046 | ID=cds.MS.gene063782.t1;Parent=MS.gene063782.t1 |
| Gene Sequence |
| Protein sequence |
