Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064319.t1 | XP_013457950.1 | 78.6 | 299 | 64 | 0 | 1 | 299 | 296 | 594 | 2.60E-77 | 298.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064319.t1 | A0A072URK3 | 78.6 | 299 | 64 | 0 | 1 | 299 | 296 | 594 | 1.9e-77 | 298.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene064319 | 0.81189 | 5.58E-51 | -1.69E-46 |
| MS.gene049368 | MS.gene064319 | 0.821448 | 4.02E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene064319 | 0.808246 | 3.40E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene064319 | 0.842558 | 2.40E-58 | -1.69E-46 |
| MS.gene050414 | MS.gene064319 | 0.802356 | 5.83E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene064319 | 0.833392 | 5.48E-56 | -1.69E-46 |
| MS.gene050654 | MS.gene064319 | 0.824739 | 6.85E-54 | -1.69E-46 |
| MS.gene052016 | MS.gene064319 | 0.826373 | 2.81E-54 | -1.69E-46 |
| MS.gene052463 | MS.gene064319 | 0.838742 | 2.40E-57 | -1.69E-46 |
| MS.gene053228 | MS.gene064319 | 0.80254 | 5.35E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene064319 | 0.815155 | 1.07E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene064319 | 0.800662 | 1.30E-48 | -1.69E-46 |
| MS.gene057174 | MS.gene064319 | 0.806825 | 6.82E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene064319 | 0.804719 | 1.89E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene064319 | 0.806187 | 9.29E-50 | -1.69E-46 |
| MS.gene060522 | MS.gene064319 | 0.813973 | 1.95E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene064319 | 0.824394 | 8.26E-54 | -1.69E-46 |
| MS.gene060933 | MS.gene064319 | 0.812747 | 3.63E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene064319 | 0.801605 | 8.32E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene064319 | 0.816401 | 5.64E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene064319 | 0.821324 | 4.29E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 56 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCGTGTCCAACATGTTTA+AGG | 0.172552 | 8.2:+16797327 | None:intergenic |
| ATCCCCTTCTGTTTGACTTT+TGG | 0.224890 | 8.2:+16797901 | None:intergenic |
| CTCTGGTACATTGTCATTGC+TGG | 0.226879 | 8.2:+16797517 | None:intergenic |
| CCAACTATTGTGCTCATCTT+TGG | 0.255755 | 8.2:+16797092 | None:intergenic |
| TTGTCATTGCTGGCCAGTTT+AGG | 0.306277 | 8.2:+16797527 | None:intergenic |
| TTCTTCACACGAAGAGAATA+TGG | 0.308365 | 8.2:+16797929 | None:intergenic |
| GTTGGACACGGAACTGTATC+TGG | 0.333493 | 8.2:-16797317 | MS.gene064319:CDS |
| AAATCATTATTATCAAGTTC+TGG | 0.357077 | 8.2:-16797782 | MS.gene064319:CDS |
| TCTTCTATCAGAAAACAAAA+TGG | 0.376900 | 8.2:-16797185 | MS.gene064319:CDS |
| TGATTTATCTTTGCCTCCAT+TGG | 0.382995 | 8.2:+16797736 | None:intergenic |
| TGATTTATCCTTGCCTCCAC+TGG | 0.386533 | 8.2:+16797673 | None:intergenic |
| AAGTCAAACAGAAGGGGATT+TGG | 0.387227 | 8.2:-16797897 | MS.gene064319:CDS |
| TGGTGAAGTCAAACCTAAAC+TGG | 0.395719 | 8.2:-16797540 | MS.gene064319:CDS |
| AAATCATTATCATCAAGTTC+TGG | 0.412208 | 8.2:-16797719 | MS.gene064319:CDS |
| CAAAGATGAGCACAATAGTT+GGG | 0.419518 | 8.2:-16797091 | MS.gene064319:CDS |
| TGAGGGTAGGGTACAAAATC+AGG | 0.424794 | 8.2:-16797115 | MS.gene064319:CDS |
| GCATTATCACTTGAGCTTGA+AGG | 0.428307 | 8.2:+16797605 | None:intergenic |
| AAGAGGAGTAATAAGAAAAC+AGG | 0.430892 | 8.2:-16797383 | MS.gene064319:CDS |
| CGGAGATGTCTGCATCTTCA+AGG | 0.448210 | 8.2:-16797355 | MS.gene064319:CDS |
| GGTTTAGTTGCTTCACAAGC+AGG | 0.465694 | 8.2:+16797440 | None:intergenic |
| AACATCTTCCACTGGAGGTA+AGG | 0.486687 | 8.2:-16797807 | MS.gene064319:CDS |
| CCAAAGATGAGCACAATAGT+TGG | 0.488426 | 8.2:-16797092 | MS.gene064319:CDS |
| ACCTTCACCTTCACCATCAA+AGG | 0.511038 | 8.2:-16797417 | MS.gene064319:CDS |
| TCCTTTGATGGTGAAGGTGA+AGG | 0.521139 | 8.2:+16797416 | None:intergenic |
| GATGTCTGCATCTTCAAGGA+AGG | 0.526687 | 8.2:-16797351 | MS.gene064319:CDS |
| TGATTTATCCTTACCTCCAG+TGG | 0.528306 | 8.2:+16797799 | None:intergenic |
| AACAAAATGGTACTACTACT+AGG | 0.529189 | 8.2:-16797172 | MS.gene064319:CDS |
| CAAGGTGAAAATGTGAGGGT+AGG | 0.536261 | 8.2:-16797128 | MS.gene064319:CDS |
| AACATCTTCCAGTGGAGGCA+AGG | 0.543748 | 8.2:-16797681 | MS.gene064319:CDS |
| AGGAAGGGCCTTAAACATGT+TGG | 0.552923 | 8.2:-16797335 | MS.gene064319:CDS |
| GTTGCTTCACAAGCAGGAGT+AGG | 0.554925 | 8.2:+16797446 | None:intergenic |
| ACTACTAGGCTCAACAAGAC+AGG | 0.555324 | 8.2:-16797158 | MS.gene064319:CDS |
| TCTGATAGAAGAGTTAGATG+AGG | 0.562628 | 8.2:+16797196 | None:intergenic |
| TAATAAGAAAACAGGTAAGT+CGG | 0.566833 | 8.2:-16797375 | MS.gene064319:CDS |
| ACAAACGATAACGTTGATGA+TGG | 0.569068 | 8.2:+16797221 | None:intergenic |
| AAGGTGAAAATGTGAGGGTA+GGG | 0.571945 | 8.2:-16797127 | MS.gene064319:CDS |
| TTTGATGGTGAAGGTGAAGG+TGG | 0.585800 | 8.2:+16797419 | None:intergenic |
| CGTTCCAAAAGTCAAACAGA+AGG | 0.591335 | 8.2:-16797905 | MS.gene064319:CDS |
| AACAAGACAGGACGTAAACA+AGG | 0.596781 | 8.2:-16797146 | MS.gene064319:CDS |
| CAGAAAATAACATCTTCCAC+TGG | 0.599320 | 8.2:-16797815 | MS.gene064319:CDS |
| CAAAGGACAAAATCTTGAAG+AGG | 0.603908 | 8.2:-16797400 | MS.gene064319:CDS |
| TGTTCTGAATCTGTTCGTTG+AGG | 0.613062 | 8.2:+16797277 | None:intergenic |
| GTTCCAAAAGTCAAACAGAA+GGG | 0.613416 | 8.2:-16797904 | MS.gene064319:CDS |
| AAAATAACATCTTCCAATGG+AGG | 0.616105 | 8.2:-16797749 | MS.gene064319:CDS |
| ATGTCTGCATCTTCAAGGAA+GGG | 0.616569 | 8.2:-16797350 | MS.gene064319:CDS |
| GTAAACAAGGTGAAAATGTG+AGG | 0.617107 | 8.2:-16797133 | MS.gene064319:CDS |
| CAGAAAATAACATCTTCCAA+TGG | 0.617194 | 8.2:-16797752 | MS.gene064319:CDS |
| GAAACAGTACGCGCTTTATG+CGG | 0.626523 | 8.2:+16797248 | None:intergenic |
| AAAGATGAGCACAATAGTTG+GGG | 0.627817 | 8.2:-16797090 | MS.gene064319:CDS |
| TTCCAAAAGTCAAACAGAAG+GGG | 0.632650 | 8.2:-16797903 | MS.gene064319:CDS |
| AGCATAAAAGAAACTGCAAG+CGG | 0.637729 | 8.2:-16797845 | MS.gene064319:CDS |
| GGCCTTAAACATGTTGGACA+CGG | 0.638832 | 8.2:-16797329 | MS.gene064319:CDS |
| AAAATAACATCTTCCAGTGG+AGG | 0.646533 | 8.2:-16797686 | MS.gene064319:CDS |
| AAAATAACATCTTCCACTGG+AGG | 0.652576 | 8.2:-16797812 | MS.gene064319:CDS |
| TAAACAAGGTGAAAATGTGA+GGG | 0.681565 | 8.2:-16797132 | MS.gene064319:CDS |
| CAGAAAATAACATCTTCCAG+TGG | 0.684375 | 8.2:-16797689 | MS.gene064319:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATCATTATTATCAAGTTC+TGG | - | chr8.2:16797251-16797270 | MS.gene064319:CDS | 20.0% |
| ! | AAATCATTATCATCAAGTTC+TGG | - | chr8.2:16797314-16797333 | MS.gene064319:CDS | 25.0% |
| ! | TAATAAGAAAACAGGTAAGT+CGG | - | chr8.2:16797658-16797677 | MS.gene064319:CDS | 25.0% |
| ! | TCTTCTATCAGAAAACAAAA+TGG | - | chr8.2:16797848-16797867 | MS.gene064319:CDS | 25.0% |
| !! | AGACACTCTTTTAGATTTTT+TGG | + | chr8.2:16797453-16797472 | None:intergenic | 25.0% |
| AAAATAACATCTTCCAATGG+AGG | - | chr8.2:16797284-16797303 | MS.gene064319:CDS | 30.0% | |
| AACAAAATGGTACTACTACT+AGG | - | chr8.2:16797861-16797880 | MS.gene064319:CDS | 30.0% | |
| AAGAGGAGTAATAAGAAAAC+AGG | - | chr8.2:16797650-16797669 | MS.gene064319:CDS | 30.0% | |
| CAGAAAATAACATCTTCCAA+TGG | - | chr8.2:16797281-16797300 | MS.gene064319:CDS | 30.0% | |
| TAAACAAGGTGAAAATGTGA+GGG | - | chr8.2:16797901-16797920 | MS.gene064319:CDS | 30.0% | |
| ! | ATGACATGCAAAATTTTCTC+TGG | + | chr8.2:16797536-16797555 | None:intergenic | 30.0% |
| ! | TCACCATTCTCATGATTTTT+GGG | + | chr8.2:16797479-16797498 | None:intergenic | 30.0% |
| ! | TCAGATGAATTTTCTGAGTT+AGG | + | chr8.2:16797563-16797582 | None:intergenic | 30.0% |
| ! | TTCAAGATTTTGTCCTTTGA+TGG | + | chr8.2:16797632-16797651 | None:intergenic | 30.0% |
| ! | TTCACCATTCTCATGATTTT+TGG | + | chr8.2:16797480-16797499 | None:intergenic | 30.0% |
| AAAATAACATCTTCCACTGG+AGG | - | chr8.2:16797221-16797240 | MS.gene064319:CDS | 35.0% | |
| AAAATAACATCTTCCAGTGG+AGG | - | chr8.2:16797347-16797366 | MS.gene064319:CDS | 35.0% | |
| AAAGATGAGCACAATAGTTG+GGG | - | chr8.2:16797943-16797962 | MS.gene064319:CDS | 35.0% | |
| AGCATAAAAGAAACTGCAAG+CGG | - | chr8.2:16797188-16797207 | MS.gene064319:CDS | 35.0% | |
| CAAAGATGAGCACAATAGTT+GGG | - | chr8.2:16797942-16797961 | MS.gene064319:CDS | 35.0% | |
| CAAAGGACAAAATCTTGAAG+AGG | - | chr8.2:16797633-16797652 | MS.gene064319:CDS | 35.0% | |
| CAGAAAATAACATCTTCCAC+TGG | - | chr8.2:16797218-16797237 | MS.gene064319:CDS | 35.0% | |
| CAGAAAATAACATCTTCCAG+TGG | - | chr8.2:16797344-16797363 | MS.gene064319:CDS | 35.0% | |
| GTAAACAAGGTGAAAATGTG+AGG | - | chr8.2:16797900-16797919 | MS.gene064319:CDS | 35.0% | |
| GTGAAAAACAAATGTGCCTT+TGG | - | chr8.2:16797158-16797177 | MS.gene064319:CDS | 35.0% | |
| GTTCCAAAAGTCAAACAGAA+GGG | - | chr8.2:16797129-16797148 | MS.gene064319:CDS | 35.0% | |
| TCTCCCAAAAATCATGAGAA+TGG | - | chr8.2:16797473-16797492 | MS.gene064319:CDS | 35.0% | |
| TCTGATAGAAGAGTTAGATG+AGG | + | chr8.2:16797840-16797859 | None:intergenic | 35.0% | |
| TGATTTATCTTTGCCTCCAT+TGG | + | chr8.2:16797300-16797319 | None:intergenic | 35.0% | |
| TTCCAAAAGTCAAACAGAAG+GGG | - | chr8.2:16797130-16797149 | MS.gene064319:CDS | 35.0% | |
| TTCTTCACACGAAGAGAATA+TGG | + | chr8.2:16797107-16797126 | None:intergenic | 35.0% | |
| ! | ACAAACGATAACGTTGATGA+TGG | + | chr8.2:16797815-16797834 | None:intergenic | 35.0% |
| !! | TTTTATGCTGCTTGAACCAA+AGG | + | chr8.2:16797177-16797196 | None:intergenic | 35.0% |
| !!! | ATTTTGTCCTTTGATGGTGA+AGG | + | chr8.2:16797626-16797645 | None:intergenic | 35.0% |
| AACAAGACAGGACGTAAACA+AGG | - | chr8.2:16797887-16797906 | MS.gene064319:CDS | 40.0% | |
| AAGGTGAAAATGTGAGGGTA+GGG | - | chr8.2:16797906-16797925 | MS.gene064319:CDS | 40.0% | |
| AAGTCAAACAGAAGGGGATT+TGG | - | chr8.2:16797136-16797155 | MS.gene064319:CDS | 40.0% | |
| ATGTCTGCATCTTCAAGGAA+GGG | - | chr8.2:16797683-16797702 | MS.gene064319:CDS | 40.0% | |
| CCAAAGATGAGCACAATAGT+TGG | - | chr8.2:16797941-16797960 | MS.gene064319:CDS | 40.0% | |
| CCAACTATTGTGCTCATCTT+TGG | + | chr8.2:16797944-16797963 | None:intergenic | 40.0% | |
| CGTTCCAAAAGTCAAACAGA+AGG | - | chr8.2:16797128-16797147 | MS.gene064319:CDS | 40.0% | |
| GCATTATCACTTGAGCTTGA+AGG | + | chr8.2:16797431-16797450 | None:intergenic | 40.0% | |
| TGATTTATCCTTACCTCCAG+TGG | + | chr8.2:16797237-16797256 | None:intergenic | 40.0% | |
| TGGTGAAGTCAAACCTAAAC+TGG | - | chr8.2:16797493-16797512 | MS.gene064319:CDS | 40.0% | |
| TTCCGTGTCCAACATGTTTA+AGG | + | chr8.2:16797709-16797728 | None:intergenic | 40.0% | |
| ! | TGTTCTGAATCTGTTCGTTG+AGG | + | chr8.2:16797759-16797778 | None:intergenic | 40.0% |
| !! | ATCCCCTTCTGTTTGACTTT+TGG | + | chr8.2:16797135-16797154 | None:intergenic | 40.0% |
| AACATCTTCCACTGGAGGTA+AGG | - | chr8.2:16797226-16797245 | MS.gene064319:CDS | 45.0% | |
| ACCTTCACCTTCACCATCAA+AGG | - | chr8.2:16797616-16797635 | MS.gene064319:CDS | 45.0% | |
| ACTACTAGGCTCAACAAGAC+AGG | - | chr8.2:16797875-16797894 | MS.gene064319:CDS | 45.0% | |
| AGGAAGGGCCTTAAACATGT+TGG | - | chr8.2:16797698-16797717 | MS.gene064319:CDS | 45.0% | |
| CAAGGTGAAAATGTGAGGGT+AGG | - | chr8.2:16797905-16797924 | MS.gene064319:CDS | 45.0% | |
| CTCTGGTACATTGTCATTGC+TGG | + | chr8.2:16797519-16797538 | None:intergenic | 45.0% | |
| GAAACAGTACGCGCTTTATG+CGG | + | chr8.2:16797788-16797807 | None:intergenic | 45.0% | |
| GATGTCTGCATCTTCAAGGA+AGG | - | chr8.2:16797682-16797701 | MS.gene064319:CDS | 45.0% | |
| GGCCTTAAACATGTTGGACA+CGG | - | chr8.2:16797704-16797723 | MS.gene064319:CDS | 45.0% | |
| GGTTTAGTTGCTTCACAAGC+AGG | + | chr8.2:16797596-16797615 | None:intergenic | 45.0% | |
| TGAGGGTAGGGTACAAAATC+AGG | - | chr8.2:16797918-16797937 | MS.gene064319:CDS | 45.0% | |
| TGATTTATCCTTGCCTCCAC+TGG | + | chr8.2:16797363-16797382 | None:intergenic | 45.0% | |
| TTGTCATTGCTGGCCAGTTT+AGG | + | chr8.2:16797509-16797528 | None:intergenic | 45.0% | |
| !! | TCCTTTGATGGTGAAGGTGA+AGG | + | chr8.2:16797620-16797639 | None:intergenic | 45.0% |
| !! | TTTGATGGTGAAGGTGAAGG+TGG | + | chr8.2:16797617-16797636 | None:intergenic | 45.0% |
| AACATCTTCCAGTGGAGGCA+AGG | - | chr8.2:16797352-16797371 | MS.gene064319:CDS | 50.0% | |
| CGGAGATGTCTGCATCTTCA+AGG | - | chr8.2:16797678-16797697 | MS.gene064319:CDS | 50.0% | |
| GTTGCTTCACAAGCAGGAGT+AGG | + | chr8.2:16797590-16797609 | None:intergenic | 50.0% | |
| GTTGGACACGGAACTGTATC+TGG | - | chr8.2:16797716-16797735 | MS.gene064319:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 16797079 | 16797976 | 16797079 | ID=MS.gene064319 |
| chr8.2 | mRNA | 16797079 | 16797976 | 16797079 | ID=MS.gene064319.t1;Parent=MS.gene064319 |
| chr8.2 | exon | 16797079 | 16797976 | 16797079 | ID=MS.gene064319.t1.exon1;Parent=MS.gene064319.t1 |
| chr8.2 | CDS | 16797079 | 16797976 | 16797079 | ID=cds.MS.gene064319.t1;Parent=MS.gene064319.t1 |
| Gene Sequence |
| Protein sequence |
