AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065365

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065365.t1 MTR_7g099750 85.652 460 27 3 1 440 404 844 0.0 766
MS.gene065365.t1 MTR_7g099800 80.726 441 82 3 1 440 413 851 0.0 717
MS.gene065365.t1 MTR_7g099820 47.255 419 180 12 1 412 409 793 9.08e-110 342
MS.gene065365.t1 MTR_7g099820 47.923 313 128 8 1 311 409 688 4.43e-83 270
MS.gene065365.t1 MTR_5g009770 40.786 407 198 14 1 404 280 646 3.84e-78 256
MS.gene065365.t1 MTR_4g123583 41.205 415 201 15 2 407 409 789 2.31e-77 256
MS.gene065365.t1 MTR_5g009770 40.786 407 198 14 1 404 411 777 7.84e-77 255
MS.gene065365.t1 MTR_4g078120 39.048 420 200 15 2 405 409 788 5.81e-75 250
MS.gene065365.t1 MTR_5g009800 39.558 407 203 12 1 404 392 758 3.09e-73 245
MS.gene065365.t1 MTR_8g093780 36.779 416 232 12 1 412 411 799 3.44e-71 240
MS.gene065365.t1 MTR_5g009780 38.821 407 208 13 1 404 411 779 1.71e-69 235
MS.gene065365.t1 MTR_2g011520 35.627 407 223 11 1 404 394 764 3.03e-65 224
MS.gene065365.t1 MTR_3g096380 36.473 414 217 16 1 404 413 790 1.28e-63 220
MS.gene065365.t1 MTR_1g043400 32.452 416 241 11 1 406 402 787 9.43e-48 175
MS.gene065365.t1 MTR_6g053320 30.189 424 220 15 1 408 407 770 4.01e-47 173
MS.gene065365.t1 MTR_1g045870 31.971 416 243 11 1 406 387 772 1.92e-46 171
MS.gene065365.t1 MTR_3g037270 29.730 407 245 11 5 404 417 789 1.93e-45 169
MS.gene065365.t1 MTR_3g031170 33.413 416 199 18 1 404 430 779 1.97e-45 168
MS.gene065365.t1 MTR_3g037260 30.095 422 225 11 5 404 414 787 3.83e-45 168
MS.gene065365.t1 MTR_5g074340 32.287 446 231 18 3 427 427 822 1.78e-44 166
MS.gene065365.t1 MTR_3g031030 30.843 415 226 18 1 404 401 765 2.29e-44 165
MS.gene065365.t1 MTR_2g040100 31.382 427 227 17 1 404 427 810 5.49e-44 164
MS.gene065365.t1 MTR_5g074360 31.873 411 217 18 3 404 426 782 2.67e-43 162
MS.gene065365.t1 MTR_4g037755 30.340 412 238 14 2 404 398 769 7.17e-42 158
MS.gene065365.t1 MTR_5g074320 29.452 438 246 14 6 431 359 745 6.10e-41 155
MS.gene065365.t1 MTR_3g071990 29.095 409 230 13 1 404 331 684 1.30e-40 154
MS.gene065365.t1 MTR_3g071990 29.095 409 230 13 1 404 413 766 1.83e-40 154
MS.gene065365.t1 MTR_5g067690 28.747 407 249 10 5 404 420 792 6.12e-40 152
MS.gene065365.t1 MTR_5g074380 30.370 405 232 13 2 404 471 827 8.68e-40 152
MS.gene065365.t1 MTR_0002s1180 28.395 405 251 10 6 404 415 786 5.80e-38 147
MS.gene065365.t1 MTR_3g031160 30.751 413 220 19 1 404 423 778 1.71e-37 145
MS.gene065365.t1 MTR_3g110700 30.163 368 221 13 48 404 433 775 5.65e-37 144
MS.gene065365.t1 MTR_4g029520 30.435 368 222 15 48 404 434 778 8.14e-37 143
MS.gene065365.t1 MTR_6g015940 27.363 402 213 11 16 404 451 786 2.66e-35 139
MS.gene065365.t1 MTR_5g088840 28.070 399 213 12 19 404 479 816 2.38e-31 127
MS.gene065365.t1 MTR_8g085260 25.926 405 216 11 16 404 454 790 1.31e-30 125
MS.gene065365.t1 MTR_3g071870 27.427 412 238 15 8 402 432 799 2.73e-30 124
MS.gene065365.t1 MTR_3g498715 27.949 390 221 11 15 399 454 788 6.70e-30 123
MS.gene065365.t1 MTR_3g071880 28.504 421 241 14 1 402 411 790 1.84e-29 122
MS.gene065365.t1 MTR_7g052680 28.426 394 224 14 16 404 475 815 3.01e-29 121
MS.gene065365.t1 MTR_5g088790 28.900 391 226 13 16 404 475 815 1.07e-28 119
MS.gene065365.t1 MTR_7g053000 28.389 391 228 12 16 404 475 815 6.49e-28 117
MS.gene065365.t1 MTR_7g052970 28.389 391 228 13 16 404 475 815 6.73e-28 117
MS.gene065365.t1 MTR_3g465680 27.818 417 223 20 1 404 565 916 2.62e-27 115
MS.gene065365.t1 MTR_5g083560 27.606 355 189 11 66 406 1 301 2.81e-27 112
MS.gene065365.t1 MTR_7g085870 25.301 415 233 13 15 403 431 794 3.28e-26 112
MS.gene065365.t1 MTR_5g088430 26.650 394 234 13 16 404 446 789 1.35e-24 107
MS.gene065365.t1 MTR_5g088470 25.510 392 241 12 16 404 446 789 1.03e-23 104
MS.gene065365.t1 MTR_5g088450 24.937 397 240 14 16 404 446 792 1.10e-22 101
MS.gene065365.t1 MTR_3g083850 26.463 393 227 13 19 404 448 785 2.73e-21 97.4
MS.gene065365.t1 MTR_5g061460 47.475 99 49 3 52 149 15 111 7.37e-19 84.0
MS.gene065365.t1 MTR_1g082340 47.475 99 49 3 52 149 82 178 3.07e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065365.t1 AT3G53720 55.288 416 173 8 1 411 342 749 6.78e-153 452
MS.gene065365.t1 AT3G53720 55.288 416 173 8 1 411 415 822 5.05e-152 452
MS.gene065365.t1 AT5G41610 40.777 412 207 14 1 407 341 720 6.07e-80 262
MS.gene065365.t1 AT5G41610 40.777 412 207 14 1 407 409 788 1.89e-79 262
MS.gene065365.t1 AT5G41610 40.777 412 207 14 1 407 409 788 1.89e-79 262
MS.gene065365.t1 AT3G17630 41.422 408 192 12 1 405 410 773 5.72e-77 255
MS.gene065365.t1 AT3G17630 41.422 408 192 12 1 405 410 773 5.72e-77 255
MS.gene065365.t1 AT4G23700 37.621 412 225 12 1 404 408 795 3.54e-75 251
MS.gene065365.t1 AT4G23700 37.621 412 225 12 1 404 408 795 3.54e-75 251
MS.gene065365.t1 AT2G13620 36.322 435 232 16 1 424 413 813 4.02e-71 240
MS.gene065365.t1 AT1G64170 37.441 422 199 15 1 404 418 792 1.09e-70 239
MS.gene065365.t1 AT1G05580 33.413 416 227 11 1 407 317 691 4.35e-52 187
MS.gene065365.t1 AT1G05580 33.413 416 227 11 1 407 428 802 1.48e-51 186
MS.gene065365.t1 AT2G31910 31.084 415 234 13 1 404 317 690 2.12e-43 162
MS.gene065365.t1 AT2G31910 31.084 415 234 13 1 404 317 690 2.12e-43 162
MS.gene065365.t1 AT2G31910 30.843 415 235 13 1 404 414 787 5.63e-43 162
MS.gene065365.t1 AT5G22900 29.340 409 230 11 11 408 462 822 7.55e-43 161
MS.gene065365.t1 AT1G06970 28.010 407 225 11 11 405 436 786 2.08e-39 151
MS.gene065365.t1 AT2G28180 29.854 412 231 12 1 404 448 809 5.29e-38 147
MS.gene065365.t1 AT1G79400 30.208 384 217 14 38 404 424 773 7.89e-37 144
MS.gene065365.t1 AT3G44900 29.540 413 228 13 3 404 453 813 8.00e-37 144
MS.gene065365.t1 AT3G52080 29.882 425 234 13 1 404 404 785 4.42e-35 138
MS.gene065365.t1 AT1G16380 28.271 428 236 15 13 404 383 775 1.01e-34 137
MS.gene065365.t1 AT2G30240 27.317 410 238 12 1 404 431 786 1.18e-34 137
MS.gene065365.t1 AT1G08150 27.179 390 236 11 1 388 451 794 6.25e-31 126
MS.gene065365.t1 AT3G44910 27.750 400 206 15 1 388 424 752 8.35e-31 125
MS.gene065365.t1 AT2G28170 26.161 409 243 13 3 404 443 799 3.67e-28 118
MS.gene065365.t1 AT3G44920 26.406 409 237 12 1 400 423 776 7.46e-28 117
MS.gene065365.t1 AT1G08135 27.248 367 219 12 40 405 477 796 4.59e-27 114
MS.gene065365.t1 AT3G44930 27.342 395 227 14 1 388 423 764 1.02e-26 114
MS.gene065365.t1 AT1G08140 26.555 418 218 16 11 404 452 804 1.22e-26 113
MS.gene065365.t1 AT5G01690 26.997 363 199 11 50 403 462 767 3.13e-26 112
MS.gene065365.t1 AT5G22910 26.764 411 214 14 1 388 435 781 1.77e-25 110
MS.gene065365.t1 AT5G58460 25.527 427 249 15 1 404 448 828 5.42e-25 108
MS.gene065365.t1 AT5G37060 27.146 431 240 15 1 404 447 830 6.94e-25 108
MS.gene065365.t1 AT5G01680 27.005 374 210 12 50 411 461 783 1.26e-20 95.1

Find 118 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCCTTGGGTCGAAGAATT+TGG 0.177714 7.4:-10470502 MS.gene065365:CDS
TATAACTTTGGTCGGTATTT+TGG 0.197869 7.4:+10470298 None:intergenic
AAAACACAATGCATATTCTT+TGG 0.224822 7.4:+10470460 None:intergenic
ATTTGCAATCATGGTACTTA+TGG 0.235524 7.4:-10471177 None:intergenic
GTAAGGTTCGTTAAGCAAAA+TGG 0.281124 7.4:-10470360 MS.gene065365:CDS
GGGTCGGTTTCCGTCGATTA+TGG 0.297648 7.4:-10469437 MS.gene065365:CDS
GGCCTTGGGTCGAAGAATTT+GGG 0.319048 7.4:-10470501 MS.gene065365:CDS
GTGGAGGAGGTGATAACATT+AGG 0.326606 7.4:-10469496 MS.gene065365:CDS
TGGAGGAGGTGATAACATTA+GGG 0.347823 7.4:-10469495 MS.gene065365:CDS
TCGAAGAATTTGGGCTTAGA+TGG 0.351131 7.4:-10470492 MS.gene065365:CDS
GTGGATAGAGGGTATGGCCT+TGG 0.354346 7.4:-10470516 MS.gene065365:CDS
AAACACAATGCATATTCTTT+GGG 0.364907 7.4:+10470461 None:intergenic
TCTAGAGTTGGGAAAGAAAA+TGG 0.365515 7.4:-10470412 MS.gene065365:CDS
TCATCTGACCTCTAGCCAAT+TGG 0.378132 7.4:-10470787 MS.gene065365:CDS
CTTCCAGCCGCCAATGTTTG+TGG 0.395185 7.4:+10470655 None:intergenic
CATCTGACCTCTAGCCAATT+GGG 0.413601 7.4:-10470786 MS.gene065365:CDS
AGAGCTGAACATGCAGAGTT+AGG 0.414940 7.4:-10469394 MS.gene065365:CDS
TCTAGAGCCTCGCGATCGTC+AGG 0.421711 7.4:+10470429 None:intergenic
GGATGTTAAAATGTCTCCTA+TGG 0.422501 7.4:+10469369 None:intergenic
GATTATGATCTCATAGTTGT+TGG 0.424223 7.4:-10469463 MS.gene065365:CDS
AGTGGATAGGTGTGAACAAA+AGG 0.426668 7.4:-10470575 MS.gene065365:CDS
TGGATAGAGGGTATGGCCTT+GGG 0.427895 7.4:-10470515 MS.gene065365:CDS
TAAGGTTCGTTAAGCAAAAT+GGG 0.429831 7.4:-10470359 MS.gene065365:CDS
GCCTAGCTTGCGAATTGTCT+TGG 0.430427 7.4:+10471085 None:intergenic
GGACTATAACCTTACCCAAT+TGG 0.435071 7.4:+10470772 None:intergenic
ATTGCAGGAGTTGGATGAAA+AGG 0.435105 7.4:-10469587 MS.gene065365:intron
GAATGGCATAACCGTATTGT+TGG 0.437995 7.4:-10470816 MS.gene065365:CDS
AATGTTGCAGAGGTTTCATC+TGG 0.442709 7.4:-10469244 MS.gene065365:CDS
CTGTTGCAGTGACCGTTGTA+AGG 0.444376 7.4:-10470377 MS.gene065365:CDS
GCCATTCTTCACGATTGAAC+CGG 0.446177 7.4:+10470832 None:intergenic
TCAATGAGGTTGATGATAGA+TGG 0.446239 7.4:+10470981 None:intergenic
CGACTGAGAAGCTATAACTT+TGG 0.446493 7.4:+10470286 None:intergenic
GTGCTTGTGGATAGAGGGTA+TGG 0.447224 7.4:-10470522 MS.gene065365:CDS
CGAATCCTTGCTTGCATCCA+CGG 0.447450 7.4:-10471017 MS.gene065365:CDS
GATCGCGAGGCTCTAGAGTT+GGG 0.448022 7.4:-10470423 MS.gene065365:CDS
GTGGGTGTGCTTGTGGATAG+AGG 0.452134 7.4:-10470528 MS.gene065365:CDS
AAGATATTGCCGTAGTGGAC+TGG 0.452334 7.4:+10470751 None:intergenic
AAAGAAAATGGCTGAACATC+CGG 0.454095 7.4:-10470400 MS.gene065365:CDS
CGATTGAACCGGTTGAAGAA+AGG 0.456626 7.4:+10470843 None:intergenic
TTGCTCTGTGGGTGTGCTTG+TGG 0.463322 7.4:-10470535 MS.gene065365:CDS
AATGGCATAACCGTATTGTT+GGG 0.475293 7.4:-10470815 MS.gene065365:CDS
TTGTAGATTGCCATTACTAG+TGG 0.481587 7.4:+10471128 None:intergenic
ACAAGCACACCCACAGAGCA+AGG 0.488232 7.4:+10470537 None:intergenic
CATGCAGTGTCGGAGAATGC+TGG 0.494770 7.4:-10470603 MS.gene065365:CDS
TACTAGTGGGGTTGTGATGA+AGG 0.500111 7.4:+10471142 None:intergenic
TGTAGATTGCCATTACTAGT+GGG 0.500399 7.4:+10471129 None:intergenic
GCTGCATGATGAAGAGTTTG+AGG 0.503536 7.4:+10470931 None:intergenic
GACCATCTTGCTTCCTGTTG+AGG 0.504129 7.4:+10469348 None:intergenic
CATCACAACCCCACTAGTAA+TGG 0.506614 7.4:-10471138 MS.gene065365:CDS
TTGGTTGTGATGCCACGAGC+TGG 0.507153 7.4:+10471104 None:intergenic
AATGAACAGACAAATAGAAC+AGG 0.510887 7.4:-10470259 MS.gene065365:intron
GACCTCTAGCCAATTGGGTA+AGG 0.512856 7.4:-10470781 MS.gene065365:CDS
GTTGATGATAGATGGAATGT+TGG 0.513785 7.4:+10470989 None:intergenic
CGATCGCGAGGCTCTAGAGT+TGG 0.514013 7.4:-10470424 MS.gene065365:CDS
TACCCTTCCACAAACATTGG+CGG 0.517678 7.4:-10470662 MS.gene065365:CDS
ACGTTCATCTTCCATCATCA+TGG 0.521585 7.4:-10470892 MS.gene065365:CDS
ACCGGTTCAATCGTGAAGAA+TGG 0.523081 7.4:-10470833 MS.gene065365:CDS
TGGTGGCAACATTGTGGAGG+AGG 0.531388 7.4:-10469509 MS.gene065365:CDS
CAGCCGCCAATGTTTGTGGA+AGG 0.534361 7.4:+10470659 None:intergenic
ACAGTGAAGTATATTGAGAA+GGG 0.536313 7.4:-10469532 MS.gene065365:CDS
AACAGTGAAGTATATTGAGA+AGG 0.538998 7.4:-10469533 MS.gene065365:CDS
CAGCATTCTCCGACACTGCA+TGG 0.539157 7.4:+10470604 None:intergenic
TGATCTCATAGTTGTTGGAA+AGG 0.540917 7.4:-10469458 MS.gene065365:CDS
AAGAATGCTCCTTGCTCTGT+GGG 0.541846 7.4:-10470546 MS.gene065365:CDS
GATGAAAAGCCCCAACAATA+CGG 0.545639 7.4:+10470805 None:intergenic
GATCTCATAGTTGTTGGAAA+GGG 0.546686 7.4:-10469457 MS.gene065365:CDS
TTGAACCGTGGATGCAAGCA+AGG 0.551323 7.4:+10471012 None:intergenic
GTTGGATGAAAAGGCAATGG+AGG 0.552444 7.4:-10469578 MS.gene065365:CDS
AGCGCGATGAACCATGATGA+TGG 0.557522 7.4:+10470881 None:intergenic
TCATAGTTGTTGGAAAGGGT+CGG 0.558024 7.4:-10469453 MS.gene065365:CDS
AAGTATATTGAGAAGGGTGG+TGG 0.558637 7.4:-10469526 MS.gene065365:CDS
TGGGTGTGCTTGTGGATAGA+GGG 0.559960 7.4:-10470527 MS.gene065365:CDS
TATTACCCTTCCACAAACAT+TGG 0.562447 7.4:-10470665 MS.gene065365:CDS
GTTAAGCAAAATGGGATGAT+CGG 0.563847 7.4:-10470351 MS.gene065365:CDS
ACCAAGACAATTCGCAAGCT+AGG 0.566811 7.4:-10471086 MS.gene065365:CDS
GATGTTAAAATGTCTCCTAT+GGG 0.567000 7.4:+10469370 None:intergenic
ACAACGGTCACTGCAACAGC+CGG 0.568380 7.4:+10470381 None:intergenic
CTTCCACAAACATTGGCGGC+TGG 0.568610 7.4:-10470658 MS.gene065365:CDS
GTAGATTGCCATTACTAGTG+GGG 0.570753 7.4:+10471130 None:intergenic
AAGGATTCGTAGCTCATCTA+CGG 0.572758 7.4:+10471031 None:intergenic
TTCTCTTTCCACAATGCATG+AGG 0.573921 7.4:-10470730 MS.gene065365:CDS
CATGCAGAGTTAGGTCCCAT+AGG 0.574828 7.4:-10469385 MS.gene065365:CDS
AGCCGCCAATGTTTGTGGAA+GGG 0.575299 7.4:+10470660 None:intergenic
AGCCCAAATTCTTCGACCCA+AGG 0.579740 7.4:+10470499 None:intergenic
GTGAAGTATATTGAGAAGGG+TGG 0.580487 7.4:-10469529 MS.gene065365:CDS
GGAGTTGGATGAAAAGGCAA+TGG 0.581954 7.4:-10469581 MS.gene065365:CDS
CATGCAGCTTGTTGAGCTCA+CGG 0.583649 7.4:-10470916 MS.gene065365:CDS
GTGGATAGGTGTGAACAAAA+GGG 0.600503 7.4:-10470574 MS.gene065365:CDS
TGGTGGACCTGACGATCGCG+AGG 0.602058 7.4:-10470436 MS.gene065365:CDS
ATGGCATAACCGTATTGTTG+GGG 0.602645 7.4:-10470814 MS.gene065365:CDS
GCATGATGAAGAGTTTGAGG+AGG 0.606679 7.4:+10470934 None:intergenic
AAGAGAAGATATTGCCGTAG+TGG 0.611985 7.4:+10470746 None:intergenic
TGAGAAGCTATAACTTTGGT+CGG 0.613013 7.4:+10470290 None:intergenic
ATCCTCAACAGGAAGCAAGA+TGG 0.613824 7.4:-10469350 MS.gene065365:CDS
TCGGTTTCCGTCGATTATGG+TGG 0.617439 7.4:-10469434 MS.gene065365:CDS
GAAGGGTGGTGGCAACATTG+TGG 0.620112 7.4:-10469515 MS.gene065365:CDS
GGTTATAGTCCAGTCCACTA+CGG 0.621273 7.4:-10470760 MS.gene065365:CDS
TGCACCGATGTACACGGTCA+AGG 0.621391 7.4:-10469278 MS.gene065365:CDS
TGCACCTTGACCGTGTACAT+CGG 0.621452 7.4:+10469274 None:intergenic
CAATGAGTTCCATGCAGTGT+CGG 0.622584 7.4:-10470613 MS.gene065365:CDS
AACCTTACCCAATTGGCTAG+AGG 0.625874 7.4:+10470779 None:intergenic
AAAGAATGCTCCTTGCTCTG+TGG 0.627753 7.4:-10470547 MS.gene065365:CDS
CGGAGAATGCTGGAAATGAG+TGG 0.631644 7.4:-10470593 MS.gene065365:CDS
GGGTGGTGGCAACATTGTGG+AGG 0.632253 7.4:-10469512 MS.gene065365:CDS
GGAGGAGGTGATAACATTAG+GGG 0.634008 7.4:-10469494 MS.gene065365:CDS
GCATATATCCTCATGCATTG+TGG 0.634054 7.4:+10470722 None:intergenic
GCAATCTACAAACCAGCTCG+TGG 0.634509 7.4:-10471116 MS.gene065365:CDS
CATGATGAAGAGTTTGAGGA+GGG 0.638126 7.4:+10470935 None:intergenic
AGTTCTGCCACCATAATCGA+CGG 0.645279 7.4:+10469427 None:intergenic
AATGCTGGAAATGAGTGGAT+AGG 0.649241 7.4:-10470588 MS.gene065365:CDS
AGAAGATGCACCGATGTACA+CGG 0.653659 7.4:-10469284 MS.gene065365:CDS
TTGCTTAACGAACCTTACAA+CGG 0.660135 7.4:+10470365 None:intergenic
TAGTGGGGTTGTGATGAAGG+TGG 0.664951 7.4:+10471145 None:intergenic
TGCTACGAGTTGATTCAATG+AGG 0.672348 7.4:+10470967 None:intergenic
GAGGAGTTTCAAAGCAAGTG+TGG 0.680710 7.4:-10469559 MS.gene065365:CDS
GCATGATGAGAATGTTGCAG+AGG 0.695544 7.4:-10469254 MS.gene065365:CDS
ATGGAATGTTGGTTGAACCG+TGG 0.725204 7.4:+10471000 None:intergenic
TGGTGGCAGAACTAGCAGAG+AGG 0.738029 7.4:-10469417 MS.gene065365:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAACACTAAATCTATT+TGG + chr7.4:10470151-10470170 None:intergenic 15.0%
!! TTTATTGAATGAAATTAATG+TGG - chr7.4:10470364-10470383 MS.gene065365:CDS 15.0%
!!! ATTTTTGTCTTTAGAGTTTA+AGG + chr7.4:10469325-10469344 None:intergenic 20.0%
!!! TAAAAATCTCTGAAAATAGT+TGG + chr7.4:10470682-10470701 None:intergenic 20.0%
! AAAACACAATGCATATTCTT+TGG + chr7.4:10469920-10469939 None:intergenic 25.0%
! AAACACAATGCATATTCTTT+GGG + chr7.4:10469919-10469938 None:intergenic 25.0%
! TATGTTTATAGTCCTAATTG+AGG - chr7.4:10470169-10470188 MS.gene065365:intron 25.0%
!! CACTTTTGTTAATTAATTGC+AGG - chr7.4:10470775-10470794 MS.gene065365:CDS 25.0%
!!! AGAATATGCATTGTGTTTTT+TGG - chr7.4:10469921-10469940 MS.gene065365:intron 25.0%
AACAGTGAAGTATATTGAGA+AGG - chr7.4:10470844-10470863 MS.gene065365:CDS 30.0%
AAGCTACAATTTCCTCAATT+AGG + chr7.4:10470184-10470203 None:intergenic 30.0%
AATGAACAGACAAATAGAAC+AGG - chr7.4:10470118-10470137 MS.gene065365:intron 30.0%
ACAGTGAAGTATATTGAGAA+GGG - chr7.4:10470845-10470864 MS.gene065365:CDS 30.0%
GATGTTAAAATGTCTCCTAT+GGG + chr7.4:10471010-10471029 None:intergenic 30.0%
GATTATGATCTCATAGTTGT+TGG - chr7.4:10470914-10470933 MS.gene065365:CDS 30.0%
TAAGGTTCGTTAAGCAAAAT+GGG - chr7.4:10470018-10470037 MS.gene065365:intron 30.0%
TGTTAATTAATTGCAGGAGT+TGG - chr7.4:10470781-10470800 MS.gene065365:CDS 30.0%
! CTATCACTCACTCTTTTATT+GGG + chr7.4:10470446-10470465 None:intergenic 30.0%
! TATAACTTTGGTCGGTATTT+TGG + chr7.4:10470082-10470101 None:intergenic 30.0%
! TCTATCACTCACTCTTTTAT+TGG + chr7.4:10470447-10470466 None:intergenic 30.0%
!!! ATATGCATTGTGTTTTTTGG+TGG - chr7.4:10469924-10469943 MS.gene065365:intron 30.0%
AAAATGGAACCCACATAAAG+TGG - chr7.4:10470400-10470419 MS.gene065365:CDS 35.0%
AAAGAAAATGGCTGAACATC+CGG - chr7.4:10469977-10469996 MS.gene065365:intron 35.0%
AATGGCATAACCGTATTGTT+GGG - chr7.4:10469562-10469581 MS.gene065365:CDS 35.0%
GATCTCATAGTTGTTGGAAA+GGG - chr7.4:10470920-10470939 MS.gene065365:CDS 35.0%
GGATGTTAAAATGTCTCCTA+TGG + chr7.4:10471011-10471030 None:intergenic 35.0%
GTAAGGTTCGTTAAGCAAAA+TGG - chr7.4:10470017-10470036 MS.gene065365:intron 35.0%
GTTAAGCAAAATGGGATGAT+CGG - chr7.4:10470026-10470045 MS.gene065365:intron 35.0%
TATTACCCTTCCACAAACAT+TGG - chr7.4:10469712-10469731 MS.gene065365:intron 35.0%
TGAGAAGCTATAACTTTGGT+CGG + chr7.4:10470090-10470109 None:intergenic 35.0%
TGATCTCATAGTTGTTGGAA+AGG - chr7.4:10470919-10470938 MS.gene065365:CDS 35.0%
TGTAGATTGCCATTACTAGT+GGG + chr7.4:10469251-10469270 None:intergenic 35.0%
TTCTCAGTACAAAGTTAGCT+AGG - chr7.4:10470202-10470221 MS.gene065365:intron 35.0%
TTGCTTAACGAACCTTACAA+CGG + chr7.4:10470015-10470034 None:intergenic 35.0%
TTGTAGATTGCCATTACTAG+TGG + chr7.4:10469252-10469271 None:intergenic 35.0%
! ATGGTTTTCCTTTCTTCAAC+CGG - chr7.4:10469526-10469545 MS.gene065365:CDS 35.0%
! TCTAGAGTTGGGAAAGAAAA+TGG - chr7.4:10469965-10469984 MS.gene065365:intron 35.0%
! TGGAACCTACACTTTGAAAA+TGG - chr7.4:10470384-10470403 MS.gene065365:CDS 35.0%
!! GACATTTTAACATCCTCAAC+AGG - chr7.4:10471016-10471035 MS.gene065365:CDS 35.0%
!! GTTGATGATAGATGGAATGT+TGG + chr7.4:10469391-10469410 None:intergenic 35.0%
!! TCAATGAGGTTGATGATAGA+TGG + chr7.4:10469399-10469418 None:intergenic 35.0%
AAGAGAAGATATTGCCGTAG+TGG + chr7.4:10469634-10469653 None:intergenic 40.0%
AAGGATTCGTAGCTCATCTA+CGG + chr7.4:10469349-10469368 None:intergenic 40.0%
AAGTATATTGAGAAGGGTGG+TGG - chr7.4:10470851-10470870 MS.gene065365:CDS 40.0%
AATGCTGGAAATGAGTGGAT+AGG - chr7.4:10469789-10469808 MS.gene065365:intron 40.0%
AATGTTGCAGAGGTTTCATC+TGG - chr7.4:10471133-10471152 MS.gene065365:CDS 40.0%
ACGTTCATCTTCCATCATCA+TGG - chr7.4:10469485-10469504 MS.gene065365:CDS 40.0%
AGTGGATAGGTGTGAACAAA+AGG - chr7.4:10469802-10469821 MS.gene065365:intron 40.0%
ATGGCATAACCGTATTGTTG+GGG - chr7.4:10469563-10469582 MS.gene065365:CDS 40.0%
ATTGCAGGAGTTGGATGAAA+AGG - chr7.4:10470790-10470809 MS.gene065365:CDS 40.0%
CATGATGAAGAGTTTGAGGA+GGG + chr7.4:10469445-10469464 None:intergenic 40.0%
CGACTGAGAAGCTATAACTT+TGG + chr7.4:10470094-10470113 None:intergenic 40.0%
GAATGGCATAACCGTATTGT+TGG - chr7.4:10469561-10469580 MS.gene065365:CDS 40.0%
GATGAAAAGCCCCAACAATA+CGG + chr7.4:10469575-10469594 None:intergenic 40.0%
GCATATATCCTCATGCATTG+TGG + chr7.4:10469658-10469677 None:intergenic 40.0%
GGACTATAACCTTACCCAAT+TGG + chr7.4:10469608-10469627 None:intergenic 40.0%
GTAGATTGCCATTACTAGTG+GGG + chr7.4:10469250-10469269 None:intergenic 40.0%
GTGAAGTATATTGAGAAGGG+TGG - chr7.4:10470848-10470867 MS.gene065365:CDS 40.0%
GTGGATAGGTGTGAACAAAA+GGG - chr7.4:10469803-10469822 MS.gene065365:intron 40.0%
TATGACCCACCACTTTATGT+GGG + chr7.4:10470412-10470431 None:intergenic 40.0%
TCAGTACAAAGTTAGCTAGG+AGG - chr7.4:10470205-10470224 MS.gene065365:intron 40.0%
TCATAGTTGTTGGAAAGGGT+CGG - chr7.4:10470924-10470943 MS.gene065365:CDS 40.0%
TCGAAGAATTTGGGCTTAGA+TGG - chr7.4:10469885-10469904 MS.gene065365:intron 40.0%
TGGAGGAGGTGATAACATTA+GGG - chr7.4:10470882-10470901 MS.gene065365:CDS 40.0%
TTCTCTTTCCACAATGCATG+AGG - chr7.4:10469647-10469666 MS.gene065365:intron 40.0%
! GGGTTCCATTTTCAAAGTGT+AGG + chr7.4:10470392-10470411 None:intergenic 40.0%
!! TGCTACGAGTTGATTCAATG+AGG + chr7.4:10469413-10469432 None:intergenic 40.0%
AAAGAATGCTCCTTGCTCTG+TGG - chr7.4:10469830-10469849 MS.gene065365:intron 45.0%
AACCTTACCCAATTGGCTAG+AGG + chr7.4:10469601-10469620 None:intergenic 45.0%
AAGAATGCTCCTTGCTCTGT+GGG - chr7.4:10469831-10469850 MS.gene065365:intron 45.0%
ACCAAGACAATTCGCAAGCT+AGG - chr7.4:10469291-10469310 MS.gene065365:CDS 45.0%
AGAGCTGAACATGCAGAGTT+AGG - chr7.4:10470983-10471002 MS.gene065365:CDS 45.0%
AGTTCTGCCACCATAATCGA+CGG + chr7.4:10470953-10470972 None:intergenic 45.0%
ATCCTCAACAGGAAGCAAGA+TGG - chr7.4:10471027-10471046 MS.gene065365:CDS 45.0%
ATGGAACCCACATAAAGTGG+TGG - chr7.4:10470403-10470422 MS.gene065365:CDS 45.0%
CAATGAGTTCCATGCAGTGT+CGG - chr7.4:10469764-10469783 MS.gene065365:intron 45.0%
CATCACAACCCCACTAGTAA+TGG - chr7.4:10469239-10469258 MS.gene065365:CDS 45.0%
CATCTGACCTCTAGCCAATT+GGG - chr7.4:10469591-10469610 MS.gene065365:intron 45.0%
CGATTGAACCGGTTGAAGAA+AGG + chr7.4:10469537-10469556 None:intergenic 45.0%
GAGGAGTTTCAAAGCAAGTG+TGG - chr7.4:10470818-10470837 MS.gene065365:CDS 45.0%
GCATGATGAAGAGTTTGAGG+AGG + chr7.4:10469446-10469465 None:intergenic 45.0%
GCATGATGAGAATGTTGCAG+AGG - chr7.4:10471123-10471142 MS.gene065365:CDS 45.0%
GCCATTCTTCACGATTGAAC+CGG + chr7.4:10469548-10469567 None:intergenic 45.0%
GCTGCATGATGAAGAGTTTG+AGG + chr7.4:10469449-10469468 None:intergenic 45.0%
GGAGGAGGTGATAACATTAG+GGG - chr7.4:10470883-10470902 MS.gene065365:CDS 45.0%
GGAGTTGGATGAAAAGGCAA+TGG - chr7.4:10470796-10470815 MS.gene065365:CDS 45.0%
GGTTATAGTCCAGTCCACTA+CGG - chr7.4:10469617-10469636 MS.gene065365:intron 45.0%
GTATGACCCACCACTTTATG+TGG + chr7.4:10470413-10470432 None:intergenic 45.0%
GTGGAGGAGGTGATAACATT+AGG - chr7.4:10470881-10470900 MS.gene065365:CDS 45.0%
GTTCATCGCGCTCGTAAAAA+TGG - chr7.4:10469507-10469526 MS.gene065365:CDS 45.0%
GTTGGATGAAAAGGCAATGG+AGG - chr7.4:10470799-10470818 MS.gene065365:CDS 45.0%
TACCCTTCCACAAACATTGG+CGG - chr7.4:10469715-10469734 MS.gene065365:intron 45.0%
TACTAGTGGGGTTGTGATGA+AGG + chr7.4:10469238-10469257 None:intergenic 45.0%
TCATCTGACCTCTAGCCAAT+TGG - chr7.4:10469590-10469609 MS.gene065365:intron 45.0%
TGGAACCCACATAAAGTGGT+GGG - chr7.4:10470404-10470423 MS.gene065365:CDS 45.0%
! ACCGGTTCAATCGTGAAGAA+TGG - chr7.4:10469544-10469563 MS.gene065365:CDS 45.0%
! AGAAGATGCACCGATGTACA+CGG - chr7.4:10471093-10471112 MS.gene065365:CDS 45.0%
! ATGGAATGTTGGTTGAACCG+TGG + chr7.4:10469380-10469399 None:intergenic 45.0%
! CTCTCTTTTCGTCTGCTATG+TGG + chr7.4:10469680-10469699 None:intergenic 45.0%
!! AAGATATTGCCGTAGTGGAC+TGG + chr7.4:10469629-10469648 None:intergenic 45.0%
!!! CGGTATTTTGGTCAGGTGAT+TGG + chr7.4:10470070-10470089 None:intergenic 45.0%
!!! CTTTGGTCGGTATTTTGGTC+AGG + chr7.4:10470077-10470096 None:intergenic 45.0%
!! AAAAATATTTATACTTTAAA+AGG + chr7.4:10470502-10470521 None:intergenic 5.0%
AGCCCAAATTCTTCGACCCA+AGG + chr7.4:10469881-10469900 None:intergenic 50.0%
AGCCGCCAATGTTTGTGGAA+GGG + chr7.4:10469720-10469739 None:intergenic 50.0%
AGCGCGATGAACCATGATGA+TGG + chr7.4:10469499-10469518 None:intergenic 50.0%
CATGCAGAGTTAGGTCCCAT+AGG - chr7.4:10470992-10471011 MS.gene065365:CDS 50.0%
CGAATCCTTGCTTGCATCCA+CGG - chr7.4:10469360-10469379 MS.gene065365:CDS 50.0%
CGGAGAATGCTGGAAATGAG+TGG - chr7.4:10469784-10469803 MS.gene065365:intron 50.0%
CTGTTGCAGTGACCGTTGTA+AGG - chr7.4:10470000-10470019 MS.gene065365:intron 50.0%
GACCATCTTGCTTCCTGTTG+AGG + chr7.4:10471032-10471051 None:intergenic 50.0%
GACCTCTAGCCAATTGGGTA+AGG - chr7.4:10469596-10469615 MS.gene065365:intron 50.0%
GCAATCTACAAACCAGCTCG+TGG - chr7.4:10469261-10469280 MS.gene065365:CDS 50.0%
GGCCTTGGGTCGAAGAATTT+GGG - chr7.4:10469876-10469895 MS.gene065365:intron 50.0%
GTGCTTGTGGATAGAGGGTA+TGG - chr7.4:10469855-10469874 MS.gene065365:intron 50.0%
TAGTGGGGTTGTGATGAAGG+TGG + chr7.4:10469235-10469254 None:intergenic 50.0%
TGCACCTTGACCGTGTACAT+CGG + chr7.4:10471106-10471125 None:intergenic 50.0%
TGGGTGTGCTTGTGGATAGA+GGG - chr7.4:10469850-10469869 MS.gene065365:intron 50.0%
TTGAACCGTGGATGCAAGCA+AGG + chr7.4:10469368-10469387 None:intergenic 50.0%
! GAGTGCTCAAAAGAGAGCGT+TGG - chr7.4:10470469-10470488 MS.gene065365:CDS 50.0%
! GCCTAGCTTGCGAATTGTCT+TGG + chr7.4:10469295-10469314 None:intergenic 50.0%
! TCGGTTTCCGTCGATTATGG+TGG - chr7.4:10470943-10470962 MS.gene065365:CDS 50.0%
! TGGATAGAGGGTATGGCCTT+GGG - chr7.4:10469862-10469881 MS.gene065365:intron 50.0%
! TGGCCTTGGGTCGAAGAATT+TGG - chr7.4:10469875-10469894 MS.gene065365:intron 50.0%
!! CATGCAGCTTGTTGAGCTCA+CGG - chr7.4:10469461-10469480 MS.gene065365:CDS 50.0%
ACAACGGTCACTGCAACAGC+CGG + chr7.4:10469999-10470018 None:intergenic 55.0%
ACAAGCACACCCACAGAGCA+AGG + chr7.4:10469843-10469862 None:intergenic 55.0%
CAGCATTCTCCGACACTGCA+TGG + chr7.4:10469776-10469795 None:intergenic 55.0%
CAGCCGCCAATGTTTGTGGA+AGG + chr7.4:10469721-10469740 None:intergenic 55.0%
CTTCCACAAACATTGGCGGC+TGG - chr7.4:10469719-10469738 MS.gene065365:intron 55.0%
CTTCCAGCCGCCAATGTTTG+TGG + chr7.4:10469725-10469744 None:intergenic 55.0%
GTGGGTGTGCTTGTGGATAG+AGG - chr7.4:10469849-10469868 MS.gene065365:intron 55.0%
TGGTGGCAACATTGTGGAGG+AGG - chr7.4:10470868-10470887 MS.gene065365:CDS 55.0%
TGGTGGCAGAACTAGCAGAG+AGG - chr7.4:10470960-10470979 MS.gene065365:CDS 55.0%
TTGCTCTGTGGGTGTGCTTG+TGG - chr7.4:10469842-10469861 MS.gene065365:intron 55.0%
TTGGTTGTGATGCCACGAGC+TGG + chr7.4:10469276-10469295 None:intergenic 55.0%
! CATGCAGTGTCGGAGAATGC+TGG - chr7.4:10469774-10469793 MS.gene065365:intron 55.0%
! GAAGGGTGGTGGCAACATTG+TGG - chr7.4:10470862-10470881 MS.gene065365:CDS 55.0%
! GGGTCGGTTTCCGTCGATTA+TGG - chr7.4:10470940-10470959 MS.gene065365:CDS 55.0%
! GTGGATAGAGGGTATGGCCT+TGG - chr7.4:10469861-10469880 MS.gene065365:intron 55.0%
! TGCACCGATGTACACGGTCA+AGG - chr7.4:10471099-10471118 MS.gene065365:CDS 55.0%
!! GATCGCGAGGCTCTAGAGTT+GGG - chr7.4:10469954-10469973 MS.gene065365:intron 55.0%
! GGGTGGTGGCAACATTGTGG+AGG - chr7.4:10470865-10470884 MS.gene065365:CDS 60.0%
! TCTAGAGCCTCGCGATCGTC+AGG + chr7.4:10469951-10469970 None:intergenic 60.0%
!! CGATCGCGAGGCTCTAGAGT+TGG - chr7.4:10469953-10469972 MS.gene065365:intron 60.0%
! TGGTGGACCTGACGATCGCG+AGG - chr7.4:10469941-10469960 MS.gene065365:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 10469210 10471189 10469210 ID=MS.gene065365
chr7.4 mRNA 10469210 10471189 10469210 ID=MS.gene065365.t1;Parent=MS.gene065365
chr7.4 exon 10470260 10471189 10470260 ID=MS.gene065365.t1.exon1;Parent=MS.gene065365.t1
chr7.4 CDS 10470260 10471189 10470260 ID=cds.MS.gene065365.t1;Parent=MS.gene065365.t1
chr7.4 exon 10469210 10469602 10469210 ID=MS.gene065365.t1.exon2;Parent=MS.gene065365.t1
chr7.4 CDS 10469210 10469602 10469210 ID=cds.MS.gene065365.t1;Parent=MS.gene065365.t1
Gene Sequence

>MS.gene065365

ATGGTACTTATGGCTCTGTTCACCACCTTCATCACAACCCCACTAGTAATGGCAATCTACAAACCAGCTCGTGGCATCACAACCAAGACAATTCGCAAGCTAGGCGACATGTCCTTAAACTCTAAAGACAAAAATGCCGTAGATGAGCTACGAATCCTTGCTTGCATCCACGGTTCAACCAACATTCCATCTATCATCAACCTCATTGAATCAACTCGTAGCACAAAAAATTCCCTCCTCAAACTCTTCATCATGCAGCTTGTTGAGCTCACGGAACGTTCATCTTCCATCATCATGGTTCATCGCGCTCGTAAAAATGGTTTTCCTTTCTTCAACCGGTTCAATCGTGAAGAATGGCATAACCGTATTGTTGGGGCTTTTCATCTGACCTCTAGCCAATTGGGTAAGGTTATAGTCCAGTCCACTACGGCAATATCTTCTCTTTCCACAATGCATGAGGATATATGCCACATAGCAGACGAAAAGAGAGTGACATTGATCATATTACCCTTCCACAAACATTGGCGGCTGGAAGAAGTTGATGATGAAAATGACAATGAGTTCCATGCAGTGTCGGAGAATGCTGGAAATGAGTGGATAGGTGTGAACAAAAGGGTACTAAAGAATGCTCCTTGCTCTGTGGGTGTGCTTGTGGATAGAGGGTATGGCCTTGGGTCGAAGAATTTGGGCTTAGATGGAAATATAGCCCAAAGAATATGCATTGTGTTTTTTGGTGGACCTGACGATCGCGAGGCTCTAGAGTTGGGAAAGAAAATGGCTGAACATCCGGCTGTTGCAGTGACCGTTGTAAGGTTCGTTAAGCAAAATGGGATGATCGGTAATAATATAGTTTTACTCCAATCACCTGACCAAAATACCGACCAAAGTTATAGCTTCTCAGTCGCAAAAATGAACAGACAAATAGAACAGGTAATGCTCCAAATAGATTTAGTGTTAAATATGTTTATAGTCCTAATTGAGGAAATTGTAGCTTCTCAGTACAAAGTTAGCTAGGAGGACTATTTTGTCACATTTTCAGAAATTAGAATAAAAACAAATCATTGTCTTAGTTTTAGACTAAATATGATTATGATTTAAAAGTAGAGAAATGCTAGCAACACTCTCTTTTAAACACTCTCTCTAGCACTCACTCTTTTATTGAATGAAATTAATGTGGAACCTACACTTTGAAAATGGAACCCACATAAAGTGGTGGGTCATACATTGATTTCACCCAATAAAAGAGTGAGTGATAGAGAGAGTGCTCAAAAGAGAGCGTTGGTAGCATTCCTTTTAAAGTATAAATATTTTTTTTACACAATCAATTAATCATAGCAGTTTGATCATCGAAAATATTTGACTTTATACGCAATTAATTCTAAAGTACATGCATGAGTGATTGATAGACATCTATAAAATTTGTTACACTCATATGTTTAAGTGAACTATAATTTATTCTTGAGCATTTGCCAACTATTTTCAGAGATTTTTATTTCAAAAGAATAAGCTTGCTTACTTATGTTTAGTCATCATATATCTGTACAATTTGAATATTTTCATATAGACACTTTTGTTAATTAATTGCAGGAGTTGGATGAAAAGGCAATGGAGGAGTTTCAAAGCAAGTGTGGTGAAACAGTGAAGTATATTGAGAAGGGTGGTGGCAACATTGTGGAGGAGGTGATAACATTAGGGGAAAGCGCAGATTATGATCTCATAGTTGTTGGAAAGGGTCGGTTTCCGTCGATTATGGTGGCAGAACTAGCAGAGAGGAGAGCTGAACATGCAGAGTTAGGTCCCATAGGAGACATTTTAACATCCTCAACAGGAAGCAAGATGGTCTCATCAATACTTGTTATTCAACAACATGATGTGACATTAACAGAAGATGCACCGATGTACACGGTCAAGGTGCATGATGAGAATGTTGCAGAGGTTTCATCTGGAAGACATGAGATCACTATAGTAAATGCAATGTAA
Protein sequence

>MS.gene065365.t1

MVLMALFTTFITTPLVMAIYKPARGITTKTIRKLGDMSLNSKDKNAVDELRILACIHGSTNIPSIINLIESTRSTKNSLLKLFIMQLVELTERSSSIIMVHRARKNGFPFFNRFNREEWHNRIVGAFHLTSSQLGKVIVQSTTAISSLSTMHEDICHIADEKRVTLIILPFHKHWRLEEVDDENDNEFHAVSENAGNEWIGVNKRVLKNAPCSVGVLVDRGYGLGSKNLGLDGNIAQRICIVFFGGPDDREALELGKKMAEHPAVAVTVVRFVKQNGMIGNNIVLLQSPDQNTDQSYSFSVAKMNRQIEQELDEKAMEEFQSKCGETVKYIEKGGGNIVEEVITLGESADYDLIVVGKGRFPSIMVAELAERRAEHAELGPIGDILTSSTGSKMVSSILVIQQHDVTLTEDAPMYTVKVHDENVAEVSSGRHEITIVNAM