Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065704.t1 | RHN82252.1 | 92.3 | 52 | 4 | 0 | 1 | 52 | 457 | 508 | 2.60E-17 | 97.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065704.t1 | Q9MB73 | 57.1 | 42 | 18 | 0 | 2 | 43 | 425 | 466 | 8.1e-07 | 53.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065704.t1 | A0A072VPY3 | 92.3 | 52 | 4 | 0 | 1 | 52 | 428 | 479 | 1.9e-17 | 97.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene065704 | 0.807571 | 4.74E-50 | -1.69E-46 |
| MS.gene049983 | MS.gene065704 | 0.810895 | 9.18E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene065704 | 0.817688 | 2.90E-52 | -1.69E-46 |
| MS.gene050237 | MS.gene065704 | 0.81439 | 1.58E-51 | -1.69E-46 |
| MS.gene050657 | MS.gene065704 | 0.801197 | 1.01E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene065704 | 0.802278 | 6.05E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene065704 | 0.802406 | 5.70E-49 | -1.69E-46 |
| MS.gene050826 | MS.gene065704 | 0.804209 | 2.41E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene065704 | 0.816546 | 5.23E-52 | -1.69E-46 |
| MS.gene052561 | MS.gene065704 | 0.807196 | 5.69E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene065704 | 0.802056 | 6.72E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene065704 | 0.800618 | 1.32E-48 | -1.69E-46 |
| MS.gene05423 | MS.gene065704 | 0.812041 | 5.18E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene065704 | 0.80015 | 1.65E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene065704 | 0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene056779 | MS.gene065704 | 0.824417 | 8.16E-54 | -1.69E-46 |
| MS.gene05761 | MS.gene065704 | 0.817224 | 3.69E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene065704 | 0.809591 | 1.75E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene065704 | 0.806115 | 9.62E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene065704 | 0.812557 | 3.99E-51 | -1.69E-46 |
| MS.gene059272 | MS.gene065704 | 0.807089 | 5.99E-50 | -1.69E-46 |
| MS.gene060011 | MS.gene065704 | 0.801818 | 7.52E-49 | -1.69E-46 |
| MS.gene060840 | MS.gene065704 | 0.822556 | 2.22E-53 | -1.69E-46 |
| MS.gene060932 | MS.gene065704 | 0.811835 | 5.74E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065704.t1 | MTR_1g107350 | 92.308 | 52 | 4 | 0 | 1 | 52 | 428 | 479 | 9.86e-28 | 102 |
| MS.gene065704.t1 | MTR_1g107365 | 76.471 | 51 | 12 | 0 | 1 | 51 | 451 | 501 | 3.18e-21 | 84.3 |
| MS.gene065704.t1 | MTR_1g107360 | 75.510 | 49 | 12 | 0 | 3 | 51 | 423 | 471 | 1.64e-20 | 82.4 |
| MS.gene065704.t1 | MTR_1g107325 | 79.592 | 49 | 10 | 0 | 3 | 51 | 314 | 362 | 1.85e-20 | 81.6 |
| MS.gene065704.t1 | MTR_8g064870 | 85.714 | 42 | 6 | 0 | 1 | 42 | 433 | 474 | 3.56e-19 | 78.6 |
| MS.gene065704.t1 | MTR_3g049970 | 71.429 | 49 | 14 | 0 | 3 | 51 | 431 | 479 | 1.07e-18 | 77.4 |
| MS.gene065704.t1 | MTR_1g107375 | 82.927 | 41 | 7 | 0 | 3 | 43 | 415 | 455 | 5.78e-17 | 72.4 |
| MS.gene065704.t1 | MTR_1g107285 | 73.171 | 41 | 11 | 0 | 3 | 43 | 425 | 465 | 1.97e-15 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065704.t1 | AT3G21560 | 61.702 | 47 | 16 | 1 | 3 | 47 | 434 | 480 | 3.59e-11 | 55.8 |
Find 14 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTGTTGGAAGCAATGGC+AGG | 0.461071 | 1.4:-82727252 | None:intergenic |
| GAAGAAAGCGGCGGAGGATG+CGG | 0.501929 | 1.4:-82727190 | MS.gene065704:CDS |
| CCGATCAGATGATCCTCCTA+TGG | 0.511292 | 1.4:+82727164 | None:intergenic |
| GCGGAGGATGCGGTTGCCAT+AGG | 0.525049 | 1.4:-82727180 | MS.gene065704:CDS |
| ATGCAAGACATTAAGAAATG+TGG | 0.538804 | 1.4:-82727126 | MS.gene065704:CDS |
| GAGGATGCGGTTGCCATAGG+AGG | 0.562944 | 1.4:-82727177 | MS.gene065704:CDS |
| GAAGTGGAAGAAAGCGGCGG+AGG | 0.586120 | 1.4:-82727196 | MS.gene065704:CDS |
| GCTTGTTGGAAGCAATGGCA+GGG | 0.593680 | 1.4:-82727251 | None:intergenic |
| CTTGTTGGAAGCAATGGCAG+GGG | 0.620250 | 1.4:-82727250 | None:intergenic |
| CCATAGGAGGATCATCTGAT+CGG | 0.621876 | 1.4:-82727164 | MS.gene065704:CDS |
| AGACATTAAGAAATGTGGCA+AGG | 0.641541 | 1.4:-82727121 | MS.gene065704:CDS |
| TGCAATGAAGTGGAAGAAAG+CGG | 0.657391 | 1.4:-82727202 | MS.gene065704:CDS |
| TTGTTGGAAGCAATGGCAGG+GGG | 0.685360 | 1.4:-82727249 | None:intergenic |
| AATGAAGTGGAAGAAAGCGG+CGG | 0.736094 | 1.4:-82727199 | MS.gene065704:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGCAAGACATTAAGAAATG+TGG | - | chr1.4:82727212-82727231 | MS.gene065704:CDS | 30.0% | |
| TGAAGAAAAATGCAATGAAG+TGG | - | chr1.4:82727126-82727145 | MS.gene065704:CDS | 30.0% | |
| AGACATTAAGAAATGTGGCA+AGG | - | chr1.4:82727217-82727236 | MS.gene065704:CDS | 35.0% | |
| TGCAATGAAGTGGAAGAAAG+CGG | - | chr1.4:82727136-82727155 | MS.gene065704:CDS | 40.0% | |
| AATGAAGTGGAAGAAAGCGG+CGG | - | chr1.4:82727139-82727158 | MS.gene065704:CDS | 45.0% | |
| CCATAGGAGGATCATCTGAT+CGG | - | chr1.4:82727174-82727193 | MS.gene065704:CDS | 45.0% | |
| CCGATCAGATGATCCTCCTA+TGG | + | chr1.4:82727177-82727196 | None:intergenic | 50.0% | |
| GAAGAAAGCGGCGGAGGATG+CGG | - | chr1.4:82727148-82727167 | MS.gene065704:CDS | 60.0% | |
| GAAGTGGAAGAAAGCGGCGG+AGG | - | chr1.4:82727142-82727161 | MS.gene065704:CDS | 60.0% | |
| GAGGATGCGGTTGCCATAGG+AGG | - | chr1.4:82727161-82727180 | MS.gene065704:CDS | 60.0% | |
| GCGGAGGATGCGGTTGCCAT+AGG | - | chr1.4:82727158-82727177 | MS.gene065704:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82727101 | 82727259 | 82727101 | ID=MS.gene065704 |
| chr1.4 | mRNA | 82727101 | 82727259 | 82727101 | ID=MS.gene065704.t1;Parent=MS.gene065704 |
| chr1.4 | exon | 82727101 | 82727259 | 82727101 | ID=MS.gene065704.t1.exon1;Parent=MS.gene065704.t1 |
| chr1.4 | CDS | 82727101 | 82727259 | 82727101 | ID=cds.MS.gene065704.t1;Parent=MS.gene065704.t1 |
| Gene Sequence |
| Protein sequence |
