AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051535


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051535.t1 MTR_1g014930 98.658 149 2 0 1 149 1 149 8.26e-108 303
MS.gene051535.t1 MTR_3g105340 54.839 124 45 4 13 135 11 124 2.44e-37 124
MS.gene051535.t1 MTR_8g021190 46.400 125 54 5 20 136 34 153 4.21e-30 107
MS.gene051535.t1 MTR_4g125030 52.727 110 44 3 6 113 15 118 5.45e-30 105
MS.gene051535.t1 MTR_4g125010 48.649 111 48 3 6 113 17 121 2.14e-28 102
MS.gene051535.t1 MTR_3g109860 37.374 99 58 2 13 110 33 128 1.63e-17 74.3
MS.gene051535.t1 MTR_1g103550 45.070 71 37 1 19 89 5 73 2.66e-17 73.2
MS.gene051535.t1 MTR_5g006430 39.560 91 49 2 17 107 3 87 3.55e-17 72.8
MS.gene051535.t1 MTR_8g013990 31.724 145 82 5 1 136 47 183 6.58e-16 71.2
MS.gene051535.t1 MTR_2g101140 45.455 77 31 2 17 86 63 135 1.76e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051535.t1 AT1G72510 43.939 132 57 5 19 136 36 164 7.97e-30 106
MS.gene051535.t1 AT1G72510 43.939 132 57 5 19 136 36 164 7.97e-30 106
MS.gene051535.t1 AT5G20670 43.478 138 63 3 11 136 17 151 1.26e-28 103
MS.gene051535.t1 AT2G09970 37.931 145 71 6 6 136 26 165 4.19e-24 92.0
MS.gene051535.t1 AT1G54095 44.037 109 59 2 1 109 1 107 1.63e-20 82.4
MS.gene051535.t1 AT3G22540 37.500 120 59 5 13 131 2 106 6.24e-19 77.0
MS.gene051535.t1 AT4G14819 36.134 119 59 4 13 131 2 103 1.21e-17 73.6
MS.gene051535.t1 AT2G25780 35.537 121 70 4 20 138 39 153 3.12e-14 66.2
MS.gene051535.t1 AT1G79770 70.000 40 12 0 17 56 52 91 1.15e-13 65.1
MS.gene051535.t1 AT5G25840 37.008 127 60 6 20 136 46 162 3.06e-13 63.9

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAACTGCTGGCGAAATT+TGG 0.269881 1.3:-7159545 None:intergenic
CCGCAAAATGCAGCTGTTGC+GGG 0.402544 1.3:+7159362 MS.gene051535:CDS
CACGCAGAATGTTCCTTACT+CGG 0.438740 1.3:-7159652 None:intergenic
TTAGGACTTTGGATTCTCCT+AGG 0.457929 1.3:+7159623 MS.gene051535:CDS
TCCTAGGAGCAAGCCGAGTA+AGG 0.469773 1.3:+7159639 MS.gene051535:CDS
GGGAGAGATTCGGTGGAAGA+TGG 0.469879 1.3:+7159425 MS.gene051535:CDS
GTATGTGTCTCTGTCGTCCT+TGG 0.478341 1.3:-7159727 None:intergenic
ACCGCAAAATGCAGCTGTTG+CGG 0.482069 1.3:+7159361 MS.gene051535:CDS
CTGAGGCAGTGAAGGAAGAA+AGG 0.486207 1.3:+7159464 MS.gene051535:CDS
AGAAGCTGAAGCGACGGAGA+TGG 0.501388 1.3:+7159327 MS.gene051535:CDS
CATTCTGCGTGCAGACCTCT+TGG 0.504691 1.3:+7159664 MS.gene051535:CDS
AAGCAGAAGATCTGAACTGC+TGG 0.512505 1.3:-7159555 None:intergenic
GAAGGAAGAAAGGGCGAGGT+CGG 0.514158 1.3:+7159474 MS.gene051535:CDS
ATGCAGCTGTTGCGGGTTGG+TGG 0.517846 1.3:+7159369 MS.gene051535:CDS
TCCTTACTCGGCTTGCTCCT+AGG 0.526193 1.3:-7159640 None:intergenic
TGTGTCTCTGTCGTCCTTGG+CGG 0.528871 1.3:-7159724 None:intergenic
GCGCGCGTGAGGGAGAGATT+CGG 0.542153 1.3:+7159415 MS.gene051535:CDS
ATGCGTACATTGCGCGCGTG+AGG 0.557254 1.3:+7159404 MS.gene051535:CDS
TCATAGTTGCTTCTCAACAA+TGG 0.575206 1.3:+7159693 MS.gene051535:CDS
TGCGTACATTGCGCGCGTGA+GGG 0.578384 1.3:+7159405 MS.gene051535:CDS
TGAGGCAGTGAAGGAAGAAA+GGG 0.590842 1.3:+7159465 MS.gene051535:CDS
CAGTGAAGGAAGAAAGGGCG+AGG 0.593016 1.3:+7159470 MS.gene051535:CDS
TTTATGTGCTGAGGCAGTGA+AGG 0.603888 1.3:+7159456 MS.gene051535:CDS
CGCGTGAGGGAGAGATTCGG+TGG 0.607365 1.3:+7159418 MS.gene051535:CDS
TGTGAAAGAAGCTGAAGCGA+CGG 0.619197 1.3:+7159321 MS.gene051535:CDS
AAAATGCAGCTGTTGCGGGT+TGG 0.633011 1.3:+7159366 MS.gene051535:CDS
CAATGGAAACAACTCCGCCA+AGG 0.641855 1.3:+7159710 MS.gene051535:CDS
TTCGGTGGAAGATGGATATG+CGG 0.649354 1.3:+7159433 MS.gene051535:CDS
CAACTATGAGATCTACCAAG+AGG 0.689504 1.3:-7159679 None:intergenic
CAATTGATGAAGCTTTAATG+CGG 0.691551 1.3:+7159518 MS.gene051535:CDS
GATATGCGGTTTATGTGCTG+AGG 0.715926 1.3:+7159447 MS.gene051535:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAATTGATGAAGCTTTAATG+CGG + chr1.3:7159518-7159537 MS.gene051535:CDS 30.0%
! CTGTGAAACAGATTCTTTTT+AGG + chr1.3:7159605-7159624 MS.gene051535:CDS 30.0%
!!! ACAGATTCTTTTTAGGACTT+TGG + chr1.3:7159612-7159631 MS.gene051535:CDS 30.0%
TCATAGTTGCTTCTCAACAA+TGG + chr1.3:7159693-7159712 MS.gene051535:CDS 35.0%
CAACTATGAGATCTACCAAG+AGG - chr1.3:7159682-7159701 None:intergenic 40.0%
TTAGGACTTTGGATTCTCCT+AGG + chr1.3:7159623-7159642 MS.gene051535:CDS 40.0%
AAGCAGAAGATCTGAACTGC+TGG - chr1.3:7159558-7159577 None:intergenic 45.0%
CACGCAGAATGTTCCTTACT+CGG - chr1.3:7159655-7159674 None:intergenic 45.0%
GATATGCGGTTTATGTGCTG+AGG + chr1.3:7159447-7159466 MS.gene051535:CDS 45.0%
TCTGAACTGCTGGCGAAATT+TGG - chr1.3:7159548-7159567 None:intergenic 45.0%
TGAGGCAGTGAAGGAAGAAA+GGG + chr1.3:7159465-7159484 MS.gene051535:CDS 45.0%
TGTGAAAGAAGCTGAAGCGA+CGG + chr1.3:7159321-7159340 MS.gene051535:CDS 45.0%
TTCGGTGGAAGATGGATATG+CGG + chr1.3:7159433-7159452 MS.gene051535:CDS 45.0%
TTTATGTGCTGAGGCAGTGA+AGG + chr1.3:7159456-7159475 MS.gene051535:CDS 45.0%
AAAATGCAGCTGTTGCGGGT+TGG + chr1.3:7159366-7159385 MS.gene051535:CDS 50.0%
ACCGCAAAATGCAGCTGTTG+CGG + chr1.3:7159361-7159380 MS.gene051535:CDS 50.0%
CAATGGAAACAACTCCGCCA+AGG + chr1.3:7159710-7159729 MS.gene051535:CDS 50.0%
CTGAGGCAGTGAAGGAAGAA+AGG + chr1.3:7159464-7159483 MS.gene051535:CDS 50.0%
GTATGTGTCTCTGTCGTCCT+TGG - chr1.3:7159730-7159749 None:intergenic 50.0%
AGAAGCTGAAGCGACGGAGA+TGG + chr1.3:7159327-7159346 MS.gene051535:CDS 55.0%
CAGTGAAGGAAGAAAGGGCG+AGG + chr1.3:7159470-7159489 MS.gene051535:CDS 55.0%
CATTCTGCGTGCAGACCTCT+TGG + chr1.3:7159664-7159683 MS.gene051535:CDS 55.0%
CCGCAAAATGCAGCTGTTGC+GGG + chr1.3:7159362-7159381 MS.gene051535:CDS 55.0%
GAAGGAAGAAAGGGCGAGGT+CGG + chr1.3:7159474-7159493 MS.gene051535:CDS 55.0%
TCCTAGGAGCAAGCCGAGTA+AGG + chr1.3:7159639-7159658 MS.gene051535:CDS 55.0%
TGTGTCTCTGTCGTCCTTGG+CGG - chr1.3:7159727-7159746 None:intergenic 55.0%
! CCCGCAACAGCTGCATTTTG+CGG - chr1.3:7159365-7159384 None:intergenic 55.0%
! TCCTTACTCGGCTTGCTCCT+AGG - chr1.3:7159643-7159662 None:intergenic 55.0%
!! GGGAGAGATTCGGTGGAAGA+TGG + chr1.3:7159425-7159444 MS.gene051535:CDS 55.0%
ATGCGTACATTGCGCGCGTG+AGG + chr1.3:7159404-7159423 MS.gene051535:CDS 60.0%
TGCGTACATTGCGCGCGTGA+GGG + chr1.3:7159405-7159424 MS.gene051535:CDS 60.0%
!! ATGCAGCTGTTGCGGGTTGG+TGG + chr1.3:7159369-7159388 MS.gene051535:CDS 60.0%
CGCGTGAGGGAGAGATTCGG+TGG + chr1.3:7159418-7159437 MS.gene051535:CDS 65.0%
GCGCGCGTGAGGGAGAGATT+CGG + chr1.3:7159415-7159434 MS.gene051535:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 7159307 7159756 7159307 ID=MS.gene051535
chr1.3 mRNA 7159307 7159756 7159307 ID=MS.gene051535.t1;Parent=MS.gene051535
chr1.3 exon 7159307 7159756 7159307 ID=MS.gene051535.t1.exon1;Parent=MS.gene051535.t1
chr1.3 CDS 7159307 7159756 7159307 ID=cds.MS.gene051535.t1;Parent=MS.gene051535.t1
Gene Sequence

>MS.gene051535

ATGGCTGCGAATGTTGTGAAAGAAGCTGAAGCGACGGAGATGGAAGCTGTTTTGACCGCAAAATGCAGCTGTTGCGGGTTGGTGGAAGAGTGCACACATGCGTACATTGCGCGCGTGAGGGAGAGATTCGGTGGAAGATGGATATGCGGTTTATGTGCTGAGGCAGTGAAGGAAGAAAGGGCGAGGTCGGAGAGTGATGAGAAGATGATCACAATTGATGAAGCTTTAATGCGGCACACCAAATTTCGCCAGCAGTTCAGATCTTCTGCTTCTGATCATAACAAAGATTTCATAGTTGCTGTGAAACAGATTCTTTTTAGGACTTTGGATTCTCCTAGGAGCAAGCCGAGTAAGGAACATTCTGCGTGCAGACCTCTTGGTAGATCTCATAGTTGCTTCTCAACAATGGAAACAACTCCGCCAAGGACGACAGAGACACATACAGAGTGA

Protein sequence

>MS.gene051535.t1

MAANVVKEAEATEMEAVLTAKCSCCGLVEECTHAYIARVRERFGGRWICGLCAEAVKEERARSESDEKMITIDEALMRHTKFRQQFRSSASDHNKDFIVAVKQILFRTLDSPRSKPSKEHSACRPLGRSHSCFSTMETTPPRTTETHTE