Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066568.t1 | XP_003626280.1 | 94.3 | 193 | 11 | 0 | 1 | 193 | 218 | 410 | 7.90E-91 | 343.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066568.t1 | Q9FZ33 | 57.3 | 192 | 78 | 1 | 2 | 193 | 213 | 400 | 1.0e-54 | 214.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066568.t1 | G7KYN0 | 94.3 | 193 | 11 | 0 | 1 | 193 | 218 | 410 | 5.7e-91 | 343.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene066568 | 0.81343 | 2.57E-51 | -1.69E-46 |
| MS.gene049418 | MS.gene066568 | 0.843023 | 1.81E-58 | -1.69E-46 |
| MS.gene049824 | MS.gene066568 | 0.801044 | 1.08E-48 | -1.69E-46 |
| MS.gene049887 | MS.gene066568 | 0.821746 | 3.43E-53 | -1.69E-46 |
| MS.gene050630 | MS.gene066568 | 0.827435 | 1.57E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene066568 | 0.813465 | 2.52E-51 | -1.69E-46 |
| MS.gene050877 | MS.gene066568 | 0.814757 | 1.31E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene066568 | 0.808485 | 3.03E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene066568 | 0.821252 | 4.46E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene066568 | 0.800579 | 1.35E-48 | -1.69E-46 |
| MS.gene051720 | MS.gene066568 | 0.827038 | 1.95E-54 | -1.69E-46 |
| MS.gene052133 | MS.gene066568 | 0.806153 | 9.45E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene066568 | 0.825663 | 4.15E-54 | -1.69E-46 |
| MS.gene052732 | MS.gene066568 | 0.846179 | 2.56E-59 | -1.69E-46 |
| MS.gene053122 | MS.gene066568 | 0.803716 | 3.05E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene066568 | 0.871097 | 8.86E-67 | -1.69E-46 |
| MS.gene053174 | MS.gene066568 | 0.826605 | 2.47E-54 | -1.69E-46 |
| MS.gene053581 | MS.gene066568 | 0.81215 | 4.90E-51 | -1.69E-46 |
| MS.gene053599 | MS.gene066568 | 0.82291 | 1.84E-53 | -1.69E-46 |
| MS.gene054550 | MS.gene066568 | 0.837247 | 5.82E-57 | -1.69E-46 |
| MS.gene054584 | MS.gene066568 | 0.885729 | 6.32E-72 | -1.69E-46 |
| MS.gene055465 | MS.gene066568 | 0.824323 | 8.59E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene066568 | 0.812084 | 5.06E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene066568 | 0.81737 | 3.42E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene066568 | 0.813709 | 2.23E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene066568 | 0.848017 | 8.01E-60 | -1.69E-46 |
| MS.gene055580 | MS.gene066568 | -0.857579 | 1.48E-62 | -1.69E-46 |
| MS.gene055890 | MS.gene066568 | 0.808993 | 2.36E-50 | -1.69E-46 |
| MS.gene055891 | MS.gene066568 | 0.84498 | 5.40E-59 | -1.69E-46 |
| MS.gene056043 | MS.gene066568 | -0.844748 | 6.24E-59 | -1.69E-46 |
| MS.gene056797 | MS.gene066568 | 0.80405 | 2.60E-49 | -1.69E-46 |
| MS.gene058668 | MS.gene066568 | 0.819232 | 1.29E-52 | -1.69E-46 |
| MS.gene058852 | MS.gene066568 | 0.805249 | 1.46E-49 | -1.69E-46 |
| MS.gene05894 | MS.gene066568 | 0.80238 | 5.77E-49 | -1.69E-46 |
| MS.gene060194 | MS.gene066568 | 0.816819 | 4.54E-52 | -1.69E-46 |
| MS.gene060519 | MS.gene066568 | 0.800012 | 1.76E-48 | -1.69E-46 |
| MS.gene060587 | MS.gene066568 | 0.844331 | 8.08E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066568.t1 | MTR_7g113420 | 94.819 | 193 | 10 | 0 | 1 | 193 | 218 | 410 | 5.92e-120 | 346 |
| MS.gene066568.t1 | MTR_7g113420 | 94.819 | 193 | 10 | 0 | 1 | 193 | 218 | 410 | 1.45e-118 | 345 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066568.t1 | AT1G54990 | 60.938 | 192 | 71 | 1 | 2 | 193 | 213 | 400 | 3.40e-75 | 233 |
Find 55 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAAGTTTGTTACTCATTC+TGG | 0.188700 | 7.4:+3333394 | MS.gene066568:CDS |
| GGTTTCTGAGAATTTAAATT+TGG | 0.212168 | 7.4:+3332997 | MS.gene066568:CDS |
| GTGAATAAACCTATTGATTT+AGG | 0.213075 | 7.4:+3332869 | MS.gene066568:CDS |
| TGAAGAGATTGGAAAAGTTT+TGG | 0.215909 | 7.4:+3332895 | MS.gene066568:CDS |
| CTTTAGGGTTTACTGCAAAT+TGG | 0.234894 | 7.4:+3332975 | MS.gene066568:CDS |
| GCCCGTGCCACCTTCAAATT+CGG | 0.300130 | 7.4:+3333045 | MS.gene066568:CDS |
| AGGATTAGCTCCTGTTCATT+TGG | 0.321069 | 7.4:+3332937 | MS.gene066568:CDS |
| GGGTTGCTGGTGCTCTTCCT+TGG | 0.322738 | 7.4:+3333371 | MS.gene066568:CDS |
| GGAGAGGTTATTGCTACATT+AGG | 0.331260 | 7.4:+3332917 | MS.gene066568:CDS |
| CGCGAAGGGAATCGGGTTGC+TGG | 0.337738 | 7.4:+3333358 | MS.gene066568:CDS |
| GGAACATCCAAAACCCAAAT+AGG | 0.346484 | 7.4:-3333076 | None:intergenic |
| CATAGGGTGCTGTTGAAGTC+TGG | 0.362685 | 7.4:+3333196 | MS.gene066568:CDS |
| TCTTCAGGACCTAAATCAAT+AGG | 0.364136 | 7.4:-3332878 | None:intergenic |
| GGTTGCTGGTGCTCTTCCTT+GGG | 0.365766 | 7.4:+3333372 | MS.gene066568:CDS |
| AATGGAGTCGCGAAGGGAAT+CGG | 0.375141 | 7.4:+3333350 | MS.gene066568:CDS |
| TAGCTTTGATTTAGCTGAAT+GGG | 0.376044 | 7.4:+3333264 | MS.gene066568:CDS |
| TACTCATTCTGGAGGCCGTT+GGG | 0.379063 | 7.4:+3333405 | MS.gene066568:CDS |
| TTTAGGGTTTACTGCAAATT+GGG | 0.387005 | 7.4:+3332976 | MS.gene066568:CDS |
| AGGAAAGCGGTTGTGGCTAT+TGG | 0.398736 | 7.4:+3333226 | MS.gene066568:CDS |
| TTCTGGAGGCCGTTGGGCTC+AGG | 0.412514 | 7.4:+3333411 | MS.gene066568:CDS |
| TTCTAAGAGAATTGGTGGTT+TGG | 0.414206 | 7.4:+3333162 | MS.gene066568:CDS |
| CCCGTGCCACCTTCAAATTC+GGG | 0.415386 | 7.4:+3333046 | MS.gene066568:CDS |
| ATGGAGTCGCGAAGGGAATC+GGG | 0.426920 | 7.4:+3333351 | MS.gene066568:CDS |
| GATTTAGGTCCTGAAGAGAT+TGG | 0.432271 | 7.4:+3332884 | MS.gene066568:CDS |
| TACTGTTCTAAGAGAATTGG+TGG | 0.443206 | 7.4:+3333157 | MS.gene066568:CDS |
| TTACTCATTCTGGAGGCCGT+TGG | 0.460405 | 7.4:+3333404 | MS.gene066568:CDS |
| TCATGCAAAACCAAATGAAC+AGG | 0.463756 | 7.4:-3332947 | None:intergenic |
| ATAGGGTGCTGTTGAAGTCT+GGG | 0.467959 | 7.4:+3333197 | MS.gene066568:CDS |
| CTAGCTTTGATTTAGCTGAA+TGG | 0.469276 | 7.4:+3333263 | MS.gene066568:CDS |
| GCTGAATGGGGTGGTTCTGA+TGG | 0.473143 | 7.4:+3333277 | MS.gene066568:CDS |
| GGATGGAAGGAAAGCGGTTG+TGG | 0.475697 | 7.4:+3333219 | MS.gene066568:CDS |
| ATATGCCAATGCAACTGATC+TGG | 0.483350 | 7.4:+3333308 | MS.gene066568:CDS |
| CCCGAATTTGAAGGTGGCAC+GGG | 0.505835 | 7.4:-3333046 | None:intergenic |
| GGAAAAGCACCCGAATTTGA+AGG | 0.517603 | 7.4:-3333055 | None:intergenic |
| AACTGATCTGGTCTAGTGAT+TGG | 0.528070 | 7.4:+3333320 | MS.gene066568:CDS |
| CTAGTGATTGGTCTGTAGAA+TGG | 0.529699 | 7.4:+3333332 | MS.gene066568:CDS |
| TTTGGATGCTGATGCACATA+GGG | 0.533671 | 7.4:+3333180 | MS.gene066568:CDS |
| AGCTTTGATTTAGCTGAATG+GGG | 0.545831 | 7.4:+3333265 | MS.gene066568:CDS |
| CTAGACCAGATCAGTTGCAT+TGG | 0.554156 | 7.4:-3333313 | None:intergenic |
| GTTTGGATGCTGATGCACAT+AGG | 0.560246 | 7.4:+3333179 | MS.gene066568:CDS |
| ACCCGAATTTGAAGGTGGCA+CGG | 0.569982 | 7.4:-3333047 | None:intergenic |
| AAAACTACCTCTCTAATCAA+AGG | 0.579564 | 7.4:-3333097 | None:intergenic |
| GGATGTTCCTTTGATTAGAG+AGG | 0.589634 | 7.4:+3333090 | MS.gene066568:CDS |
| CTGTAGAATGGAGTCGCGAA+GGG | 0.589757 | 7.4:+3333344 | MS.gene066568:CDS |
| GTGCTGTTGAAGTCTGGGGA+TGG | 0.594356 | 7.4:+3333202 | MS.gene066568:CDS |
| TTTGATTTAGCTGAATGGGG+TGG | 0.595613 | 7.4:+3333268 | MS.gene066568:CDS |
| AAGTTTGTTACTCATTCTGG+AGG | 0.604446 | 7.4:+3333397 | MS.gene066568:CDS |
| TTCTAGCTAGAATGTCAAAG+AGG | 0.614198 | 7.4:+3332843 | None:intergenic |
| TCTGTAGAATGGAGTCGCGA+AGG | 0.618026 | 7.4:+3333343 | MS.gene066568:CDS |
| TGTTGAAGTCTGGGGATGGA+AGG | 0.618688 | 7.4:+3333206 | MS.gene066568:CDS |
| GTCTGGGGATGGAAGGAAAG+CGG | 0.630612 | 7.4:+3333213 | MS.gene066568:CDS |
| TGAGTAACAAACTTTGCCCA+AGG | 0.635006 | 7.4:-3333388 | None:intergenic |
| TTGTGCACACCTGAGCCCAA+CGG | 0.637219 | 7.4:-3333420 | None:intergenic |
| AAAGCACCCGAATTTGAAGG+TGG | 0.688232 | 7.4:-3333052 | None:intergenic |
| TAGGGTGCTGTTGAAGTCTG+GGG | 0.693836 | 7.4:+3333198 | MS.gene066568:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTTTCTGAGAATTTAAATT+TGG | + | chr7.4:3332997-3333016 | MS.gene066568:CDS | 25.0% |
| !!! | GTGAATAAACCTATTGATTT+AGG | + | chr7.4:3332869-3332888 | MS.gene066568:CDS | 25.0% |
| !!! | TTTTACTGTTCTAAGAGAAT+TGG | + | chr7.4:3333154-3333173 | MS.gene066568:CDS | 25.0% |
| AAAACTACCTCTCTAATCAA+AGG | - | chr7.4:3333100-3333119 | None:intergenic | 30.0% | |
| ! | AAAACTTTTCCAATCTCTTC+AGG | - | chr7.4:3332896-3332915 | None:intergenic | 30.0% |
| ! | GAAGAGATTGGAAAAGTTTT+GGG | + | chr7.4:3332896-3332915 | MS.gene066568:CDS | 30.0% |
| ! | TGAAGAGATTGGAAAAGTTT+TGG | + | chr7.4:3332895-3332914 | MS.gene066568:CDS | 30.0% |
| ! | TTGATTAGAGAGGTAGTTTT+AGG | + | chr7.4:3333100-3333119 | MS.gene066568:CDS | 30.0% |
| ! | TTTAGGGTTTACTGCAAATT+GGG | + | chr7.4:3332976-3332995 | MS.gene066568:CDS | 30.0% |
| ! | TTTTGCATGATTCAGCTTTA+GGG | + | chr7.4:3332960-3332979 | MS.gene066568:CDS | 30.0% |
| !! | ACCACTTTTGCAAAAACATA+AGG | - | chr7.4:3333130-3333149 | None:intergenic | 30.0% |
| !! | TAGCTTTGATTTAGCTGAAT+GGG | + | chr7.4:3333264-3333283 | MS.gene066568:CDS | 30.0% |
| !! | TCCTTATGTTTTTGCAAAAG+TGG | + | chr7.4:3333126-3333145 | MS.gene066568:CDS | 30.0% |
| !! | TGATTAGAGAGGTAGTTTTA+GGG | + | chr7.4:3333101-3333120 | MS.gene066568:CDS | 30.0% |
| TCATGCAAAACCAAATGAAC+AGG | - | chr7.4:3332950-3332969 | None:intergenic | 35.0% | |
| TCTTCAGGACCTAAATCAAT+AGG | - | chr7.4:3332881-3332900 | None:intergenic | 35.0% | |
| ! | AGCTTTGATTTAGCTGAATG+GGG | + | chr7.4:3333265-3333284 | MS.gene066568:CDS | 35.0% |
| ! | GTTTTGCATGATTCAGCTTT+AGG | + | chr7.4:3332959-3332978 | MS.gene066568:CDS | 35.0% |
| ! | TTCTAAGAGAATTGGTGGTT+TGG | + | chr7.4:3333162-3333181 | MS.gene066568:CDS | 35.0% |
| !! | AAGTTTGTTACTCATTCTGG+AGG | + | chr7.4:3333397-3333416 | MS.gene066568:CDS | 35.0% |
| !! | CTAGCTTTGATTTAGCTGAA+TGG | + | chr7.4:3333263-3333282 | MS.gene066568:CDS | 35.0% |
| !! | CTTTAGGGTTTACTGCAAAT+TGG | + | chr7.4:3332975-3332994 | MS.gene066568:CDS | 35.0% |
| !! | GCAAAGTTTGTTACTCATTC+TGG | + | chr7.4:3333394-3333413 | MS.gene066568:CDS | 35.0% |
| !! | TACTGTTCTAAGAGAATTGG+TGG | + | chr7.4:3333157-3333176 | MS.gene066568:CDS | 35.0% |
| !! | TGCTTTTCCTATTTGGGTTT+TGG | + | chr7.4:3333069-3333088 | MS.gene066568:CDS | 35.0% |
| AACTGATCTGGTCTAGTGAT+TGG | + | chr7.4:3333320-3333339 | MS.gene066568:CDS | 40.0% | |
| ATATGCCAATGCAACTGATC+TGG | + | chr7.4:3333308-3333327 | MS.gene066568:CDS | 40.0% | |
| GATTTAGGTCCTGAAGAGAT+TGG | + | chr7.4:3332884-3332903 | MS.gene066568:CDS | 40.0% | |
| GGAACATCCAAAACCCAAAT+AGG | - | chr7.4:3333079-3333098 | None:intergenic | 40.0% | |
| GGAGAGGTTATTGCTACATT+AGG | + | chr7.4:3332917-3332936 | MS.gene066568:CDS | 40.0% | |
| GGATGTTCCTTTGATTAGAG+AGG | + | chr7.4:3333090-3333109 | MS.gene066568:CDS | 40.0% | |
| TGAGTAACAAACTTTGCCCA+AGG | - | chr7.4:3333391-3333410 | None:intergenic | 40.0% | |
| TTTGGATGCTGATGCACATA+GGG | + | chr7.4:3333180-3333199 | MS.gene066568:CDS | 40.0% | |
| ! | ATTCGGGTGCTTTTCCTATT+TGG | + | chr7.4:3333062-3333081 | MS.gene066568:CDS | 40.0% |
| ! | TTCGGGTGCTTTTCCTATTT+GGG | + | chr7.4:3333063-3333082 | MS.gene066568:CDS | 40.0% |
| ! | TTTGATTTAGCTGAATGGGG+TGG | + | chr7.4:3333268-3333287 | MS.gene066568:CDS | 40.0% |
| !! | AGGATTAGCTCCTGTTCATT+TGG | + | chr7.4:3332937-3332956 | MS.gene066568:CDS | 40.0% |
| !! | CTAGTGATTGGTCTGTAGAA+TGG | + | chr7.4:3333332-3333351 | MS.gene066568:CDS | 40.0% |
| !!! | GATTGGAAAAGTTTTGGGAG+AGG | + | chr7.4:3332901-3332920 | MS.gene066568:CDS | 40.0% |
| CTAGACCAGATCAGTTGCAT+TGG | - | chr7.4:3333316-3333335 | None:intergenic | 45.0% | |
| GTTTGGATGCTGATGCACAT+AGG | + | chr7.4:3333179-3333198 | MS.gene066568:CDS | 45.0% | |
| ! | AAAGCACCCGAATTTGAAGG+TGG | - | chr7.4:3333055-3333074 | None:intergenic | 45.0% |
| !! | ATAGGGTGCTGTTGAAGTCT+GGG | + | chr7.4:3333197-3333216 | MS.gene066568:CDS | 45.0% |
| !! | GGAAAAGCACCCGAATTTGA+AGG | - | chr7.4:3333058-3333077 | None:intergenic | 45.0% |
| AATGGAGTCGCGAAGGGAAT+CGG | + | chr7.4:3333350-3333369 | MS.gene066568:CDS | 50.0% | |
| ACCCGAATTTGAAGGTGGCA+CGG | - | chr7.4:3333050-3333069 | None:intergenic | 50.0% | |
| AGGAAAGCGGTTGTGGCTAT+TGG | + | chr7.4:3333226-3333245 | MS.gene066568:CDS | 50.0% | |
| CTGTAGAATGGAGTCGCGAA+GGG | + | chr7.4:3333344-3333363 | MS.gene066568:CDS | 50.0% | |
| TCTGTAGAATGGAGTCGCGA+AGG | + | chr7.4:3333343-3333362 | MS.gene066568:CDS | 50.0% | |
| TGTTGAAGTCTGGGGATGGA+AGG | + | chr7.4:3333206-3333225 | MS.gene066568:CDS | 50.0% | |
| ! | TACTCATTCTGGAGGCCGTT+GGG | + | chr7.4:3333405-3333424 | MS.gene066568:CDS | 50.0% |
| ! | TTACTCATTCTGGAGGCCGT+TGG | + | chr7.4:3333404-3333423 | MS.gene066568:CDS | 50.0% |
| !! | CATAGGGTGCTGTTGAAGTC+TGG | + | chr7.4:3333196-3333215 | MS.gene066568:CDS | 50.0% |
| !! | TAGGGTGCTGTTGAAGTCTG+GGG | + | chr7.4:3333198-3333217 | MS.gene066568:CDS | 50.0% |
| ATGGAGTCGCGAAGGGAATC+GGG | + | chr7.4:3333351-3333370 | MS.gene066568:CDS | 55.0% | |
| CCCGAATTTGAAGGTGGCAC+GGG | - | chr7.4:3333049-3333068 | None:intergenic | 55.0% | |
| CCCGTGCCACCTTCAAATTC+GGG | + | chr7.4:3333046-3333065 | MS.gene066568:CDS | 55.0% | |
| GCCCGTGCCACCTTCAAATT+CGG | + | chr7.4:3333045-3333064 | MS.gene066568:CDS | 55.0% | |
| GGATGGAAGGAAAGCGGTTG+TGG | + | chr7.4:3333219-3333238 | MS.gene066568:CDS | 55.0% | |
| GGTTGCTGGTGCTCTTCCTT+GGG | + | chr7.4:3333372-3333391 | MS.gene066568:CDS | 55.0% | |
| GTCTGGGGATGGAAGGAAAG+CGG | + | chr7.4:3333213-3333232 | MS.gene066568:CDS | 55.0% | |
| ! | GTGCTGTTGAAGTCTGGGGA+TGG | + | chr7.4:3333202-3333221 | MS.gene066568:CDS | 55.0% |
| !! | GCTGAATGGGGTGGTTCTGA+TGG | + | chr7.4:3333277-3333296 | MS.gene066568:CDS | 55.0% |
| GGGTTGCTGGTGCTCTTCCT+TGG | + | chr7.4:3333371-3333390 | MS.gene066568:CDS | 60.0% | |
| ! | CGCGAAGGGAATCGGGTTGC+TGG | + | chr7.4:3333358-3333377 | MS.gene066568:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 3332854 | 3333432 | 3332854 | ID=MS.gene066568 |
| chr7.4 | mRNA | 3332854 | 3333432 | 3332854 | ID=MS.gene066568.t1;Parent=MS.gene066568 |
| chr7.4 | exon | 3332854 | 3333432 | 3332854 | ID=MS.gene066568.t1.exon1;Parent=MS.gene066568.t1 |
| chr7.4 | CDS | 3332854 | 3333432 | 3332854 | ID=cds.MS.gene066568.t1;Parent=MS.gene066568.t1 |
| Gene Sequence |
| Protein sequence |
