AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066756

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066756.t1 MTR_2g060565 63.946 147 31 4 131 277 13 137 8.15e-52 169
MS.gene066756.t1 MTR_5g046240 58.273 139 35 4 1 116 692 830 5.09e-39 147
MS.gene066756.t1 MTR_5g046240 71.429 70 12 1 281 342 1405 1474 2.24e-22 99.0
MS.gene066756.t1 MTR_1g031840 71.429 70 12 1 281 342 688 757 2.06e-22 99.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066756.t1 AT4G38440 33.884 121 71 4 1 116 732 848 5.22e-13 70.9

Find 85 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGGATAATGAAGATATTT+TGG 0.185566 2.2:-28504252 MS.gene066756:intron
GGATGTTGTGAAAATAAATT+TGG 0.187346 2.2:+28506065 None:intergenic
GTTTGGTCTTGGAGTTATTT+TGG 0.197640 2.2:-28506020 MS.gene066756:CDS
GAAGCTTTACAGGATCTTTA+TGG 0.205527 2.2:-28505061 MS.gene066756:intron
CTGATCTTTGGAGAAAATTC+TGG 0.215165 2.2:+28505813 None:intergenic
CACATGCTTCTCAGAGTTCT+TGG 0.231355 2.2:-28505879 MS.gene066756:CDS
ATCAACTCAAGTCTGATCTT+TGG 0.258724 2.2:+28505801 None:intergenic
TGAAGTATTTGTACAAAATC+AGG 0.279723 2.2:-28505097 MS.gene066756:CDS
TTATCTATCGATTTCCTTGT+TGG 0.306983 2.2:-28505123 MS.gene066756:CDS
AGGATCATTTGCATATACTA+TGG 0.316298 2.2:-28504177 MS.gene066756:CDS
TGATGGAAATTCCTCTCTTC+TGG 0.319956 2.2:-28503827 MS.gene066756:CDS
TGATTGACTTTGGCATAAAC+TGG 0.324590 2.2:-28505992 MS.gene066756:CDS
AAATAAATTTGGAAGTCTTC+TGG 0.328906 2.2:+28506076 None:intergenic
AACGGCAGGACCTGGGGATA+TGG 0.347297 2.2:-28505374 MS.gene066756:intron
TCTTGGAAGGGTGACATTGC+AGG 0.350974 2.2:-28505862 MS.gene066756:CDS
GACTTGAGTTGATCAAATAT+TGG 0.363434 2.2:-28505791 MS.gene066756:CDS
ATAAGTTATTGTTGCGCAAC+AGG 0.376055 2.2:-28504109 MS.gene066756:CDS
CTTGACAGGGCCGCGATTTG+AGG 0.381522 2.2:-28504197 MS.gene066756:CDS
TGTTGCGCAACAGGCTTGTT+GGG 0.389869 2.2:-28504100 MS.gene066756:CDS
GGAGGTTTATGTCAAGTCAT+AGG 0.391450 2.2:-28504231 MS.gene066756:CDS
CCAACTCCCACACCTGGAAT+TGG 0.406541 2.2:+28505553 None:intergenic
CTCTGCATCATCGGTCAACT+CGG 0.421455 2.2:+28506043 None:intergenic
AGGTTTCTGATGCCCTTGAC+AGG 0.426561 2.2:-28504211 MS.gene066756:CDS
TGCAGAGGAGGACCAATTCC+AGG 0.427136 2.2:-28505565 MS.gene066756:intron
TTGTTGCGCAACAGGCTTGT+TGG 0.433583 2.2:-28504101 MS.gene066756:CDS
GTGACATTGCAGGAAGCTAA+TGG 0.452398 2.2:-28505852 MS.gene066756:CDS
CCTTGCAATTTAGATGATTG+TGG 0.474589 2.2:+28505951 None:intergenic
TTCTGCGACTTCTTTGTCGT+GGG 0.475663 2.2:-28505919 MS.gene066756:CDS
TGACCGATGATGCAGAGGTT+TGG 0.480096 2.2:-28506037 MS.gene066756:CDS
GTTGTTATAGAGAGACCATT+GGG 0.482895 2.2:-28505352 MS.gene066756:CDS
CTCAAATCGCGGCCCTGTCA+AGG 0.484116 2.2:+28504198 None:intergenic
TCTGCATAACATCAATTCAA+TGG 0.493057 2.2:+28505582 None:intergenic
AAAAGAGTTGGTTGGAGTTG+AGG 0.497118 2.2:-28503873 MS.gene066756:CDS
TTTGGTTCGATGATTGACTT+TGG 0.500665 2.2:-28506002 MS.gene066756:CDS
CAGGTATGAAATATGAATGA+TGG 0.502337 2.2:+28504140 None:intergenic
ATGATGCAGAGGTTTGGTCT+TGG 0.511030 2.2:-28506031 MS.gene066756:CDS
GAGGATGAAAGAATTGATTG+AGG 0.520698 2.2:-28503854 MS.gene066756:CDS
CTCTCTTTGAGAGATTATGC+TGG 0.521446 2.2:-28504076 MS.gene066756:CDS
TTTCTGCGACTTCTTTGTCG+TGG 0.523486 2.2:-28505920 MS.gene066756:CDS
TGCTTCTCAGAGTTCTTGGA+AGG 0.525323 2.2:-28505875 MS.gene066756:CDS
GAAGTATTTGTACAAAATCA+GGG 0.529854 2.2:-28505096 MS.gene066756:CDS
AAATTCCTCTCTTCTGGCAC+AGG 0.533778 2.2:-28503821 MS.gene066756:CDS
CCACAATCATCTAAATTGCA+AGG 0.538743 2.2:-28505951 MS.gene066756:CDS
AGACCAAACCTCTGCATCAT+CGG 0.546507 2.2:+28506034 None:intergenic
GGTTGTTATAGAGAGACCAT+TGG 0.546544 2.2:-28505353 MS.gene066756:CDS
GGCACAGGTGAACAAGTTGA+AGG 0.548409 2.2:-28503806 MS.gene066756:CDS
TATGTTTAACGGCAGGACCT+GGG 0.549650 2.2:-28505381 MS.gene066756:intron
CGCAACAATAACTTATCCAC+AGG 0.553250 2.2:+28504116 None:intergenic
CACAATCATCTAAATTGCAA+GGG 0.561016 2.2:-28505950 MS.gene066756:CDS
TCTCTTTGAGAGATTATGCT+GGG 0.563044 2.2:-28504075 MS.gene066756:CDS
CAAGCAATCAACATCTGACA+TGG 0.564410 2.2:-28503899 MS.gene066756:CDS
TCAAATCGCGGCCCTGTCAA+GGG 0.565264 2.2:+28504199 None:intergenic
CATGGATGAAAAGAGTTGGT+TGG 0.567021 2.2:-28503881 MS.gene066756:CDS
ATCATCTAAATTGCAAGGGG+GGG 0.574244 2.2:-28505946 MS.gene066756:CDS
TCTGCATCATCGGTCAACTC+GGG 0.579534 2.2:+28506044 None:intergenic
GGTTTCTGATGCCCTTGACA+GGG 0.580132 2.2:-28504210 MS.gene066756:CDS
CAATAACTTATCCACAGGAC+AGG 0.581405 2.2:+28504121 None:intergenic
GGAGAAAATTCTGGAAGACA+TGG 0.586981 2.2:+28505822 None:intergenic
CGAGTTGACCGATGATGCAG+AGG 0.590086 2.2:-28506042 MS.gene066756:CDS
TCTATAACAACCATATCCCC+AGG 0.591618 2.2:+28505364 None:intergenic
GTTGATCAAATATTGGCACC+TGG 0.594014 2.2:-28505784 MS.gene066756:CDS
GAATTGATTGAGGCATGTGA+TGG 0.598135 2.2:-28503844 MS.gene066756:CDS
CAGATGTTGATTGCTTGCTA+TGG 0.601126 2.2:+28503905 None:intergenic
ATTCAGCATTATCATTGACA+TGG 0.602127 2.2:-28505158 MS.gene066756:intron
GAATTGATGTTATGCAGAGG+AGG 0.602718 2.2:-28505577 MS.gene066756:CDS
AATCATCTAAATTGCAAGGG+GGG 0.609804 2.2:-28505947 MS.gene066756:CDS
ATATGAATGATGGAAGGTGA+TGG 0.610473 2.2:+28504150 None:intergenic
CATATTTCATACCTGTCCTG+TGG 0.618580 2.2:-28504132 MS.gene066756:CDS
ATGTTTAACGGCAGGACCTG+GGG 0.629545 2.2:-28505380 MS.gene066756:intron
GTTCACCTGTGCCAGAAGAG+AGG 0.629884 2.2:+28503816 None:intergenic
AGGATACCTTGAACCTGCAG+AGG 0.638345 2.2:-28504774 MS.gene066756:intron
GCTTCTCAGAGTTCTTGGAA+GGG 0.641030 2.2:-28505874 MS.gene066756:CDS
CAAATACTTCAATTCCAACA+AGG 0.642031 2.2:+28505109 None:intergenic
TGCAAATGATCCTCAAATCG+CGG 0.643658 2.2:+28504187 None:intergenic
CTGACATGGATGAAAAGAGT+TGG 0.644103 2.2:-28503885 MS.gene066756:CDS
TGCTGGGAAACAGCAACATG+AGG 0.645299 2.2:-28504059 MS.gene066756:intron
ACATGGAACAATAAACCCAA+TGG 0.645346 2.2:+28505337 None:intergenic
ATTGAATTGATGTTATGCAG+AGG 0.645749 2.2:-28505580 MS.gene066756:CDS
TATTTGTACAAAATCAGGGT+GGG 0.645769 2.2:-28505092 MS.gene066756:CDS
ACAATCATCTAAATTGCAAG+GGG 0.648757 2.2:-28505949 MS.gene066756:CDS
AGTACTTCAAAACAGAGACA+TGG 0.650829 2.2:+28505320 None:intergenic
TATGAAATATGAATGATGGA+AGG 0.668826 2.2:+28504144 None:intergenic
CAATCATCTAAATTGCAAGG+GGG 0.693581 2.2:-28505948 MS.gene066756:CDS
TGAGAAAATTCTGTGTACAG+AGG 0.700326 2.2:+28505293 None:intergenic
GTATTTGTACAAAATCAGGG+TGG 0.744705 2.2:-28505093 MS.gene066756:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTGGTAAAAGTTAT+TGG - chr2.2:28505351-28505370 MS.gene066756:CDS 15.0%
!! ATATACTTAATTGAGAATAT+GGG - chr2.2:28505253-28505272 MS.gene066756:intron 15.0%
!! TATATACTTAATTGAGAATA+TGG - chr2.2:28505252-28505271 MS.gene066756:intron 15.0%
!!! CCAAAATAAACAAATTATTT+TGG - chr2.2:28504870-28504889 MS.gene066756:intron 15.0%
!!! CCAAAATAATTTGTTTATTT+TGG + chr2.2:28504873-28504892 None:intergenic 15.0%
!!! CTTATTTTATAGATAATGTT+AGG - chr2.2:28505542-28505561 MS.gene066756:intron 15.0%
!!! TTCAAAAATTTAAAGTAAGT+AGG + chr2.2:28504425-28504444 None:intergenic 15.0%
!! TCAACATAATCAATATTGTT+TGG + chr2.2:28505601-28505620 None:intergenic 20.0%
!!! AAATTCTTTAAAGCGTTAAA+AGG - chr2.2:28505376-28505395 MS.gene066756:intron 20.0%
!!! ATTTTATAGATAATGTTAGG+TGG - chr2.2:28505545-28505564 MS.gene066756:intron 20.0%
!!! TGTATCTTATTTGTTCTTAT+TGG - chr2.2:28505901-28505920 MS.gene066756:CDS 20.0%
!!! TTATAAAAGAAAAGTGTTTG+TGG - chr2.2:28504201-28504220 MS.gene066756:CDS 20.0%
!!! TTTTATAGATAATGTTAGGT+GGG - chr2.2:28505546-28505565 MS.gene066756:intron 20.0%
! AAATAAATTTGGAAGTCTTC+TGG + chr2.2:28503808-28503827 None:intergenic 25.0%
! ATCTAACTAAGAGATACTAA+AGG + chr2.2:28505426-28505445 None:intergenic 25.0%
! ATGAATGACTAATTCATTTG+AGG - chr2.2:28504143-28504162 MS.gene066756:CDS 25.0%
! CTTGTATCAAAGCAAAAAAT+TGG - chr2.2:28505338-28505357 MS.gene066756:CDS 25.0%
! GCATAAGAAATATTCTATGT+AGG + chr2.2:28505175-28505194 None:intergenic 25.0%
! GGATGTTGTGAAAATAAATT+TGG + chr2.2:28503819-28503838 None:intergenic 25.0%
! TAAATATAATGAAACTTGCC+AGG + chr2.2:28504982-28505001 None:intergenic 25.0%
! TTTATAGATAATGTTAGGTG+GGG - chr2.2:28505547-28505566 MS.gene066756:intron 25.0%
!! GAAGTATTTGTACAAAATCA+GGG - chr2.2:28504785-28504804 MS.gene066756:CDS 25.0%
!! TATGAAATATGAATGATGGA+AGG + chr2.2:28505740-28505759 None:intergenic 25.0%
!! TGAAGTATTTGTACAAAATC+AGG - chr2.2:28504784-28504803 MS.gene066756:CDS 25.0%
!! TGATAATGCTGAATAGAAAA+AGG + chr2.2:28504717-28504736 None:intergenic 25.0%
ACAATCATCTAAATTGCAAG+GGG - chr2.2:28503932-28503951 MS.gene066756:intron 30.0%
AGGATCATTTGCATATACTA+TGG - chr2.2:28505704-28505723 MS.gene066756:intron 30.0%
ATTCAGCATTATCATTGACA+TGG - chr2.2:28504723-28504742 MS.gene066756:intron 30.0%
ATTGAATTGATGTTATGCAG+AGG - chr2.2:28504301-28504320 MS.gene066756:intron 30.0%
CAAATACTTCAATTCCAACA+AGG + chr2.2:28504775-28504794 None:intergenic 30.0%
CACAATCATCTAAATTGCAA+GGG - chr2.2:28503931-28503950 MS.gene066756:CDS 30.0%
CAGGTATGAAATATGAATGA+TGG + chr2.2:28505744-28505763 None:intergenic 30.0%
CTTAGTTAGATTTAGCTGTT+GGG - chr2.2:28505435-28505454 MS.gene066756:intron 30.0%
TATTTGTACAAAATCAGGGT+GGG - chr2.2:28504789-28504808 MS.gene066756:CDS 30.0%
TCTGCATAACATCAATTCAA+TGG + chr2.2:28504302-28504321 None:intergenic 30.0%
TCTTAGTTAGATTTAGCTGT+TGG - chr2.2:28505434-28505453 MS.gene066756:intron 30.0%
! GAAACAATGTCCAATTTTCA+AGG - chr2.2:28504678-28504697 MS.gene066756:intron 30.0%
! GCAGGATAATGAAGATATTT+TGG - chr2.2:28505629-28505648 MS.gene066756:intron 30.0%
! TAATTATGTTTTACCTCTGC+AGG + chr2.2:28505123-28505142 None:intergenic 30.0%
!! AAAAGAAAAGTGTTTGTGGA+AGG - chr2.2:28504205-28504224 MS.gene066756:CDS 30.0%
!! ATGAACTTGAAGAGTATTCA+AGG - chr2.2:28505471-28505490 MS.gene066756:intron 30.0%
!! GACTTGAGTTGATCAAATAT+TGG - chr2.2:28504090-28504109 MS.gene066756:CDS 30.0%
!! GGATAATGAAGATATTTTGG+AGG - chr2.2:28505632-28505651 MS.gene066756:intron 30.0%
!! TTATCTATCGATTTCCTTGT+TGG - chr2.2:28504758-28504777 MS.gene066756:intron 30.0%
AAACGACCTATACCATTCAA+CGG - chr2.2:28504458-28504477 MS.gene066756:intron 35.0%
AATCATCTAAATTGCAAGGG+GGG - chr2.2:28503934-28503953 MS.gene066756:intron 35.0%
ACATGGAACAATAAACCCAA+TGG + chr2.2:28504547-28504566 None:intergenic 35.0%
AGTACTTCAAAACAGAGACA+TGG + chr2.2:28504564-28504583 None:intergenic 35.0%
ATAAGTTATTGTTGCGCAAC+AGG - chr2.2:28505772-28505791 MS.gene066756:CDS 35.0%
ATATCTTCATTATCCTGCAG+TGG + chr2.2:28505627-28505646 None:intergenic 35.0%
ATCAACTCAAGTCTGATCTT+TGG + chr2.2:28504083-28504102 None:intergenic 35.0%
ATCTTCCATCTTAATCCATC+AGG + chr2.2:28505871-28505890 None:intergenic 35.0%
CAATCATCTAAATTGCAAGG+GGG - chr2.2:28503933-28503952 MS.gene066756:intron 35.0%
CCACAATCATCTAAATTGCA+AGG - chr2.2:28503930-28503949 MS.gene066756:CDS 35.0%
CCTTGCAATTTAGATGATTG+TGG + chr2.2:28503933-28503952 None:intergenic 35.0%
GAAGCTTTACAGGATCTTTA+TGG - chr2.2:28504820-28504839 MS.gene066756:intron 35.0%
GAGGATGAAAGAATTGATTG+AGG - chr2.2:28506027-28506046 MS.gene066756:CDS 35.0%
GTAGGTGTTTGGTTGAAATA+AGG - chr2.2:28504229-28504248 MS.gene066756:CDS 35.0%
GTATTTGTACAAAATCAGGG+TGG - chr2.2:28504788-28504807 MS.gene066756:CDS 35.0%
GTTGTCAGTTCCTTGAAAAT+TGG + chr2.2:28504691-28504710 None:intergenic 35.0%
GTTGTTATAGAGAGACCATT+GGG - chr2.2:28504529-28504548 MS.gene066756:intron 35.0%
TCAAATGGAATTCAACACAG+TGG + chr2.2:28505007-28505026 None:intergenic 35.0%
TCTGAAGGTAACAAAAGTTC+AGG + chr2.2:28505066-28505085 None:intergenic 35.0%
TCTTCCATCTTAATCCATCA+GGG + chr2.2:28505870-28505889 None:intergenic 35.0%
TGAGAAAATTCTGTGTACAG+AGG + chr2.2:28504591-28504610 None:intergenic 35.0%
TGCCTTGGAACTATGTTTAA+CGG - chr2.2:28504489-28504508 MS.gene066756:intron 35.0%
TGTTTGGTACAACTACGTTA+GGG + chr2.2:28505585-28505604 None:intergenic 35.0%
TTGTTTGGTACAACTACGTT+AGG + chr2.2:28505586-28505605 None:intergenic 35.0%
! ACTAATTCATTTGAGGCAGA+AGG - chr2.2:28504150-28504169 MS.gene066756:CDS 35.0%
! ATATGAATGATGGAAGGTGA+TGG + chr2.2:28505734-28505753 None:intergenic 35.0%
! CTAATTCATTTGAGGCAGAA+GGG - chr2.2:28504151-28504170 MS.gene066756:CDS 35.0%
! CTGATCTTTGGAGAAAATTC+TGG + chr2.2:28504071-28504090 None:intergenic 35.0%
! TCTCTTTGAGAGATTATGCT+GGG - chr2.2:28505806-28505825 MS.gene066756:CDS 35.0%
! TGATTGACTTTGGCATAAAC+TGG - chr2.2:28503889-28503908 MS.gene066756:CDS 35.0%
! TTTGGTTCGATGATTGACTT+TGG - chr2.2:28503879-28503898 MS.gene066756:CDS 35.0%
! TTTTGAATGCTTTTCCCTGA+TGG - chr2.2:28505853-28505872 MS.gene066756:CDS 35.0%
!! AAAAAGCATCTACTGTACTC+TGG + chr2.2:28505316-28505335 None:intergenic 35.0%
!! ACTTGAAGAGTATTCAAGGA+TGG - chr2.2:28505475-28505494 MS.gene066756:intron 35.0%
!! CTTGAAGAGTATTCAAGGAT+GGG - chr2.2:28505476-28505495 MS.gene066756:intron 35.0%
!!! GGATACTTTTGAAGCTTTAC+AGG - chr2.2:28504810-28504829 MS.gene066756:intron 35.0%
!!! GTTTGGTCTTGGAGTTATTT+TGG - chr2.2:28503861-28503880 MS.gene066756:CDS 35.0%
!!! TTTTCCCTGATGGATTAAGA+TGG - chr2.2:28505863-28505882 MS.gene066756:CDS 35.0%
ACATTCCCTGTAACGCAAAT+TGG + chr2.2:28505940-28505959 None:intergenic 40.0%
ACTGATCCAATTTGCGTTAC+AGG - chr2.2:28505931-28505950 MS.gene066756:CDS 40.0%
ATCATCTAAATTGCAAGGGG+GGG - chr2.2:28503935-28503954 MS.gene066756:intron 40.0%
ATTAGACAGTGTATTCCAGG+TGG + chr2.2:28505034-28505053 None:intergenic 40.0%
CAAGCAATCAACATCTGACA+TGG - chr2.2:28505982-28506001 MS.gene066756:CDS 40.0%
CAATAACTTATCCACAGGAC+AGG + chr2.2:28505763-28505782 None:intergenic 40.0%
CATATTTCATACCTGTCCTG+TGG - chr2.2:28505749-28505768 MS.gene066756:intron 40.0%
CGCAACAATAACTTATCCAC+AGG + chr2.2:28505768-28505787 None:intergenic 40.0%
CTGACATGGATGAAAAGAGT+TGG - chr2.2:28505996-28506015 MS.gene066756:CDS 40.0%
CTGATCCAATTTGCGTTACA+GGG - chr2.2:28505932-28505951 MS.gene066756:CDS 40.0%
GAATTGATGTTATGCAGAGG+AGG - chr2.2:28504304-28504323 MS.gene066756:intron 40.0%
GAATTGATTGAGGCATGTGA+TGG - chr2.2:28506037-28506056 MS.gene066756:CDS 40.0%
GGAGGTTTATGTCAAGTCAT+AGG - chr2.2:28505650-28505669 MS.gene066756:intron 40.0%
GGTTGTTATAGAGAGACCAT+TGG - chr2.2:28504528-28504547 MS.gene066756:intron 40.0%
GTTGATCAAATATTGGCACC+TGG - chr2.2:28504097-28504116 MS.gene066756:CDS 40.0%
GTTGTTCCGTTGAATGGTAT+AGG + chr2.2:28504467-28504486 None:intergenic 40.0%
TCTATAACAACCATATCCCC+AGG + chr2.2:28504520-28504539 None:intergenic 40.0%
TGATGGAAATTCCTCTCTTC+TGG - chr2.2:28506054-28506073 MS.gene066756:CDS 40.0%
TGCAAATGATCCTCAAATCG+CGG + chr2.2:28505697-28505716 None:intergenic 40.0%
TGCATTAGACAGTGTATTCC+AGG + chr2.2:28505037-28505056 None:intergenic 40.0%
TGCCGTTAAACATAGTTCCA+AGG + chr2.2:28504494-28504513 None:intergenic 40.0%
TGTCTTCAACTTCTTCAGTG+TGG - chr2.2:28504263-28504282 MS.gene066756:intron 40.0%
TTATGGTGAGCTTCTCGATA+GGG - chr2.2:28504837-28504856 MS.gene066756:intron 40.0%
TTGGAACTATGTTTAACGGC+AGG - chr2.2:28504493-28504512 MS.gene066756:intron 40.0%
TTTATGGTGAGCTTCTCGAT+AGG - chr2.2:28504836-28504855 MS.gene066756:intron 40.0%
! AAAAGAGTTGGTTGGAGTTG+AGG - chr2.2:28506008-28506027 MS.gene066756:CDS 40.0%
! AATTTTCTCAGTAGCTGGAG+GGG - chr2.2:28504601-28504620 MS.gene066756:intron 40.0%
! AGAATTTTCTCAGTAGCTGG+AGG - chr2.2:28504599-28504618 MS.gene066756:intron 40.0%
! CACAGAATTTTCTCAGTAGC+TGG - chr2.2:28504596-28504615 MS.gene066756:intron 40.0%
! CAGATGTTGATTGCTTGCTA+TGG + chr2.2:28505979-28505998 None:intergenic 40.0%
! CATGGATGAAAAGAGTTGGT+TGG - chr2.2:28506000-28506019 MS.gene066756:CDS 40.0%
! CATTTTGTCCCTTAAAAGCC+AGG + chr2.2:28504118-28504137 None:intergenic 40.0%
! CTCTCTTTGAGAGATTATGC+TGG - chr2.2:28505805-28505824 MS.gene066756:CDS 40.0%
! GAATTTTCTCAGTAGCTGGA+GGG - chr2.2:28504600-28504619 MS.gene066756:intron 40.0%
! GGAGAAAATTCTGGAAGACA+TGG + chr2.2:28504062-28504081 None:intergenic 40.0%
!! AAATGCTTTTCGAGAGCTGA+AGG - chr2.2:28505087-28505106 MS.gene066756:CDS 40.0%
!! ATATTGGCACCTGGCTTTTA+AGG - chr2.2:28504106-28504125 MS.gene066756:CDS 40.0%
!! ATTATGTTGACTTCCACTGC+AGG - chr2.2:28505611-28505630 MS.gene066756:intron 40.0%
!! CTCGAAAAGCATTTCTCTGA+AGG + chr2.2:28505081-28505100 None:intergenic 40.0%
!! GGCTTTTAAGGGACAAAATG+TGG - chr2.2:28504118-28504137 MS.gene066756:CDS 40.0%
!! TATTGGCACCTGGCTTTTAA+GGG - chr2.2:28504107-28504126 MS.gene066756:CDS 40.0%
AAATTCCTCTCTTCTGGCAC+AGG - chr2.2:28506060-28506079 MS.gene066756:CDS 45.0%
AGACCAAACCTCTGCATCAT+CGG + chr2.2:28503850-28503869 None:intergenic 45.0%
ATTCCAGGTGGCAAGTCAAA+TGG + chr2.2:28505022-28505041 None:intergenic 45.0%
CACATGCTTCTCAGAGTTCT+TGG - chr2.2:28504002-28504021 MS.gene066756:intron 45.0%
GCTTCTCAGAGTTCTTGGAA+GGG - chr2.2:28504007-28504026 MS.gene066756:intron 45.0%
GTGACATTGCAGGAAGCTAA+TGG - chr2.2:28504029-28504048 MS.gene066756:intron 45.0%
TATGTTTAACGGCAGGACCT+GGG - chr2.2:28504500-28504519 MS.gene066756:intron 45.0%
TGCTTCTCAGAGTTCTTGGA+AGG - chr2.2:28504006-28504025 MS.gene066756:intron 45.0%
TTCTGCGACTTCTTTGTCGT+GGG - chr2.2:28503962-28503981 MS.gene066756:intron 45.0%
TTTCTGCGACTTCTTTGTCG+TGG - chr2.2:28503961-28503980 MS.gene066756:intron 45.0%
! AAAGTGTTTGTGGAAGGCGT+AGG - chr2.2:28504211-28504230 MS.gene066756:CDS 45.0%
! ATGATGCAGAGGTTTGGTCT+TGG - chr2.2:28503850-28503869 MS.gene066756:CDS 45.0%
! ATTCATTTGAGGCAGAAGGG+TGG - chr2.2:28504154-28504173 MS.gene066756:CDS 45.0%
! CGATGTCAACTTCTTTTGCC+TGG - chr2.2:28504961-28504980 MS.gene066756:intron 45.0%
! GTTTTACCTCTGCAGGTTCA+AGG + chr2.2:28505116-28505135 None:intergenic 45.0%
!! ATTCCATTTGACTTGCCACC+TGG - chr2.2:28505016-28505035 MS.gene066756:intron 45.0%
AGGATACCTTGAACCTGCAG+AGG - chr2.2:28505107-28505126 MS.gene066756:CDS 50.0%
AGGTTTCTGATGCCCTTGAC+AGG - chr2.2:28505670-28505689 MS.gene066756:intron 50.0%
ATGTTTAACGGCAGGACCTG+GGG - chr2.2:28504501-28504520 MS.gene066756:intron 50.0%
CACACATTGCTGCCACCTAA+AGG + chr2.2:28504635-28504654 None:intergenic 50.0%
CTATGTTTAACGGCAGGACC+TGG - chr2.2:28504499-28504518 MS.gene066756:intron 50.0%
CTCTGCATCATCGGTCAACT+CGG + chr2.2:28503841-28503860 None:intergenic 50.0%
CTTTAGGTGGCAGCAATGTG+TGG - chr2.2:28504633-28504652 MS.gene066756:intron 50.0%
GGAGGGGTAAAGAAGCCTTT+AGG - chr2.2:28504617-28504636 MS.gene066756:intron 50.0%
GGGGTAAAGAAGCCTTTAGG+TGG - chr2.2:28504620-28504639 MS.gene066756:intron 50.0%
GGTTTCTGATGCCCTTGACA+GGG - chr2.2:28505671-28505690 MS.gene066756:intron 50.0%
TCAACGGAACAACACTGCCT+TGG - chr2.2:28504474-28504493 MS.gene066756:intron 50.0%
TCTGCATCATCGGTCAACTC+GGG + chr2.2:28503840-28503859 None:intergenic 50.0%
TCTTGGAAGGGTGACATTGC+AGG - chr2.2:28504019-28504038 MS.gene066756:intron 50.0%
TGACCGATGATGCAGAGGTT+TGG - chr2.2:28503844-28503863 MS.gene066756:CDS 50.0%
TGCTGGGAAACAGCAACATG+AGG - chr2.2:28505822-28505841 MS.gene066756:CDS 50.0%
TTGACCAAAATCCTCCGCCA+GGG + chr2.2:28504363-28504382 None:intergenic 50.0%
TTGTTGCGCAACAGGCTTGT+TGG - chr2.2:28505780-28505799 MS.gene066756:CDS 50.0%
TTTGACCAAAATCCTCCGCC+AGG + chr2.2:28504364-28504383 None:intergenic 50.0%
! GGCACAGGTGAACAAGTTGA+AGG - chr2.2:28506075-28506094 MS.gene066756:CDS 50.0%
! GGCAGTGTTGTTCCGTTGAA+TGG + chr2.2:28504473-28504492 None:intergenic 50.0%
! TGTTGCGCAACAGGCTTGTT+GGG - chr2.2:28505781-28505800 MS.gene066756:CDS 50.0%
! TTGTGGAAGGCGTAGGTGTT+TGG - chr2.2:28504218-28504237 MS.gene066756:CDS 50.0%
AGGAGGACCAATTCCAGGTG+TGG - chr2.2:28504321-28504340 MS.gene066756:intron 55.0%
CCAACTCCCACACCTGGAAT+TGG + chr2.2:28504331-28504350 None:intergenic 55.0%
CCAATTCCAGGTGTGGGAGT+TGG - chr2.2:28504328-28504347 MS.gene066756:intron 55.0%
GGAGGACCAATTCCAGGTGT+GGG - chr2.2:28504322-28504341 MS.gene066756:intron 55.0%
GTTCACCTGTGCCAGAAGAG+AGG + chr2.2:28506068-28506087 None:intergenic 55.0%
TCAAATCGCGGCCCTGTCAA+GGG + chr2.2:28505685-28505704 None:intergenic 55.0%
TGACCAAAATCCTCCGCCAG+GGG + chr2.2:28504362-28504381 None:intergenic 55.0%
TGCAGAGGAGGACCAATTCC+AGG - chr2.2:28504316-28504335 MS.gene066756:intron 55.0%
! CGAGTTGACCGATGATGCAG+AGG - chr2.2:28503839-28503858 MS.gene066756:CDS 55.0%
AACGGCAGGACCTGGGGATA+TGG - chr2.2:28504507-28504526 MS.gene066756:intron 60.0%
CCAGGTGTGGGAGTTGGATG+AGG - chr2.2:28504334-28504353 MS.gene066756:intron 60.0%
CCTCATCCAACTCCCACACC+TGG + chr2.2:28504337-28504356 None:intergenic 60.0%
CTCAAATCGCGGCCCTGTCA+AGG + chr2.2:28505686-28505705 None:intergenic 60.0%
CTTGACAGGGCCGCGATTTG+AGG - chr2.2:28505684-28505703 MS.gene066756:intron 60.0%
TGAGGCAGAAGGGTGGTGAG+TGG - chr2.2:28504161-28504180 MS.gene066756:CDS 60.0%
! GGAGTTGGATGAGGTGCCCC+TGG - chr2.2:28504343-28504362 MS.gene066756:intron 65.0%
! GTGCCCCTGGCGGAGGATTT+TGG - chr2.2:28504356-28504375 MS.gene066756:intron 65.0%
! GTTGGATGAGGTGCCCCTGG+CGG - chr2.2:28504346-28504365 MS.gene066756:intron 65.0%
! GGATGAGGTGCCCCTGGCGG+AGG - chr2.2:28504349-28504368 MS.gene066756:intron 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 28503792 28506111 28503792 ID=MS.gene066756
chr2.2 mRNA 28503792 28506111 28503792 ID=MS.gene066756.t1;Parent=MS.gene066756
chr2.2 exon 28505764 28506111 28505764 ID=MS.gene066756.t1.exon1;Parent=MS.gene066756.t1
chr2.2 CDS 28505764 28506111 28505764 ID=cds.MS.gene066756.t1;Parent=MS.gene066756.t1
chr2.2 exon 28505566 28505614 28505566 ID=MS.gene066756.t1.exon2;Parent=MS.gene066756.t1
chr2.2 CDS 28505566 28505614 28505566 ID=cds.MS.gene066756.t1;Parent=MS.gene066756.t1
chr2.2 exon 28505281 28505388 28505281 ID=MS.gene066756.t1.exon3;Parent=MS.gene066756.t1
chr2.2 CDS 28505281 28505388 28505281 ID=cds.MS.gene066756.t1;Parent=MS.gene066756.t1
chr2.2 exon 28505062 28505174 28505062 ID=MS.gene066756.t1.exon4;Parent=MS.gene066756.t1
chr2.2 CDS 28505062 28505174 28505062 ID=cds.MS.gene066756.t1;Parent=MS.gene066756.t1
chr2.2 exon 28504775 28504818 28504775 ID=MS.gene066756.t1.exon5;Parent=MS.gene066756.t1
chr2.2 CDS 28504775 28504818 28504775 ID=cds.MS.gene066756.t1;Parent=MS.gene066756.t1
chr2.2 exon 28504060 28504270 28504060 ID=MS.gene066756.t1.exon6;Parent=MS.gene066756.t1
chr2.2 CDS 28504060 28504270 28504060 ID=cds.MS.gene066756.t1;Parent=MS.gene066756.t1
chr2.2 exon 28503792 28503950 28503792 ID=MS.gene066756.t1.exon7;Parent=MS.gene066756.t1
chr2.2 CDS 28503792 28503950 28503792 ID=cds.MS.gene066756.t1;Parent=MS.gene066756.t1
Gene Sequence

>MS.gene066756

ATGGAGTCTTTGACCAGAAGACTTCCAAATTTATTTTCACAACATCCCGAGTTGACCGATGATGCAGAGGTTTGGTCTTGGAGTTATTTTGGTTCGATGATTGACTTTGGCATAAACTGGATTGCAACAAGAAGTGATCCACAATCATCTAAATTGCAAGGGGGGGATATTTCTGCGACTTCTTTGTCGTGGGTGTATGCTGCTATGACTCACATGCTTCTCAGAGTTCTTGGAAGGGTGACATTGCAGGAAGCTAATGGACATGTGCCATGTCTTCCAGAATTTTCTCCAAAGATCAGACTTGAGTTGATCAAATATTGGCACCTGGCTTTTAAGGGACAAAATGTGGTGATGAATGACTAATTCATTTGAGGCAGAAGGGTGGTGAGTGGTGACACTGAAATGTCTTTTATAAAAGAAAAGTGTTTGTGGAAGGCGTAGGTGTTTGGTTGAAATAAGGTCTAAGCTTCATGTCTTCAACTTCTTCAGTGTGGCAGCACATGCAGTCCATTGAATTGATGTTATGCAGAGGAGGACCAATTCCAGGTGTGGGAGTTGGATGAGGTGCCCCTGGCGGAGGATTTTGGTCAAAAACAATTTTATCGTTTCAAACAGATGCAAGATTTCTCACCTACTTACTTTAAATTTTTGAAAATGCAACTGAAGAAACGACCTATACCATTCAACGGAACAACACTGCCTTGGAACTATGTTTAACGGCAGGACCTGGGGATATGGTTGTTATAGAGAGACCATTGGGTTTATTGTTCCATGTCTCTGTTTTGAAGTACTTTGACCTCTGTACACAGAATTTTCTCAGTAGCTGGAGGGGTAAAGAAGCCTTTAGGTGGCAGCAATGTGTGGACTTTTCTTTGTTTTAGTCATTGAAACAATGTCCAATTTTCAAGGAACTGACAACATTCCTTTTTCTATTCAGCATTATCATTGACATGGAAGTTACATTCATTATCTATCGATTTCCTTGTTGGAATTGAAGTATTTGTACAAAATCAGGGTGGGATACTTTTGAAGCTTTACAGGATCTTTATGGTGAGCTTCTCGATAGGGCAAAGTTTAACCAAAATAAACAAATTATTTTGGATGATAAAAAGCATATTGAGTTATTGAGATTCAAATTCGAGATTCACGAGAGTTGCTCGATATTTACTCGATGTCAACTTCTTTTGCCTGGCAAGTTTCATTATATTTACACCACTGTGTTGAATTCCATTTGACTTGCCACCTGGAATACACTGTCTAATGCATGTGTTCCTGAACTTTTGTTACCTTCAGAGAAATGCTTTTCGAGAGCTGAAGGATACCTTGAACCTGCAGAGGTAAAACATAATTAATTATCAGTTGTATTATAGCAGAGCACTACCTACATAGAATATTTCTTATGCTTTTGATACTTTTTTTTTAAGTTGAAGATATAAACGTCACTTGTATGTTGTAATAGTTATATACTTAATTGAGAATATGGGTGTTTAAAGTGTGTTCTAAAACATAGAAGCATCAGCTCCAGAGTACAGTAGATGCTTTTTGTCTTGTATCAAAGCAAAAAATTGGTAAAAGTTATTGGACAAATTCTTTAAAGCGTTAAAAGGAAAATTTCATTAATCTTTTATGCACCTTTAGTATCTCTTAGTTAGATTTAGCTGTTGGGAAAGTTTTTAATAATGAACTTGAAGAGTATTCAAGGATGGGAAGAATAATGAAGATGTGTCTGACGAAACAATCATTTCAAACACTTATTTTATAGATAATGTTAGGTGGGGCAATTTTATTTTCCCTAACGTAGTTGTACCAAACAATATTGATTATGTTGACTTCCACTGCAGGATAATGAAGATATTTTGGAGGTTTATGTCAAGTCATAGGTTTCTGATGCCCTTGACAGGGCCGCGATTTGAGGATCATTTGCATATACTATGGTTGTCCATCACCTTCCATCATTCATATTTCATACCTGTCCTGTGGATAAGTTATTGTTGCGCAACAGGCTTGTTGGGTCTCTCTTTGAGAGATTATGCTGGGAAACAGCAACATGAGGTGCACGCATTTTGAATGCTTTTCCCTGATGGATTAAGATGGAAGATAAGATTTTATTGTATCTTATTTGTTCTTATTGGAAATGTTACTGATCCAATTTGCGTTACAGGGAATGTTAATGAATATCATTTGCCATAGCAAGCAATCAACATCTGACATGGATGAAAAGAGTTGGTTGGAGTTGAGGATGAAAGAATTGATTGAGGCATGTGATGGAAATTCCTCTCTTCTGGCACAGGTGAACAAGTTGAAGGATGTTGCTGCTTAG
Protein sequence

>MS.gene066756.t1

MESLTRRLPNLFSQHPELTDDAEVWSWSYFGSMIDFGINWIATRSDPQSSKLQGGDISATSLSWVYAAMTHMLLRVLGRVTLQEANGHVPCLPEFSPKIRLELIKYWHLAFKGQNVHMQSIELMLCRGGPIPGPGDMVVIERPLGLLFHVSVLKYFDLCTQNFLSSWRALSLTWKLHSLSIDFLVGIEVFVQNQGGILLKLYRIFMRNAFRELKDTLNLQRIMKIFWRFMSSHRFLMPLTGPRFEDHLHILWLSITFHHSYFIPVLWISYCCATGLLGLSLRDYAGKQQHEGMLMNIICHSKQSTSDMDEKSWLELRMKELIEACDGNSSLLAQVNKLKDVAA